Leica DM750 User Manual
Leica DM750
Manual
Manufacturer information
Published in November 2010 by:
Leica Microsystems (Schweiz) AG
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)
Responsible for contents:
Dietmar Gnass (Product Compliance)
Vincent Vaccarelli (Product Management)
In case of questions, please contact the hotline:
mqm-hotline@leica-microsystems.com
Leica DM750 Manual 2
Chapter overview
Safety regulations 5
The Leica DM750 17
Ready! 20
Set! 30
Go! 43
Care of the Microscope 45
Troubleshooting 48
Dimensions 51
Leica DM750 Manual 3
Contents
Safety regulations 5
Safety concept 6
Symbols used in this instruction manual 7
Important Notes 8
Intended Purpose of the Microscope 9
Instructions on use 10
Health risks and dangers of use 12
Information for the person responsible for the instrument 13
Care instructions 14
Accessories, maintenance and repair 15
Electrical data and ambient conditions 16
The Leica DM750 17
Introduction 18
Unpacking 19
Ready! 20
Substage Illumination 21
Attaching the viewing Tubes 22
Leica EZ Viewing Tube – integrated eyepieces 23
Leica Standard Viewing Tube – separated eyepieces 25
Eyecups 26
Installing Objectives 27
Installing the Substage Condenser 28
Set! 30
Turning on the microscope 31
Complete Condenser Centering 32
Using the Condenser 33
Prepare to View a Specimen Slide 34
Focusing 35
Viewing Tube Adjustment 36
Koehler Configuration 38
Oil Immersion Technique 40
Time Delay Shutoff 42
Go! 43
Ready! Set! Go! 44
Care of the Microscope 45
General maintenance 46
Troubleshooting 48
Troubleshooting 49
Dimensions 51
Leica DM750 Manual 4
Safety regulations
Leica DM750 Manual 5
Safety concept
The individual modules of the Leica DM
microscopy series include an interactive
CD-ROM with all relevant user manuals in
several languages. Keep it in a safe place, and
readily accessible to the user. User manuals and
updates are also available for you to download
and print from our web site at
www.leica-microsystems.com.
This user manual describes the special functions of the individual modules of the Leica
DM microscopy series and contains important
instructions for their operational safety, maintenance, and accessories.
The "Safety concept" booklet contains additional safety information regarding the service
work, requirements and the handling of
microscope, accessories and electrical accessories as well as general safety instructions.
You can combine individual system articles with
articles from external suppliers. Please read the
user manual and the safety requirements of the
supplier.
Before installing, operating or using the instruments, read the user manuals listed above. In
particular, please observe all safety instructions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Leica DM750 Manual 6
Symbols used in this instruction manual
Warning of a danger
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
Ϙ Hazards to personnel
Ϙ Functional disturbances or damaged inst-
ruments
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
Ϙ Hazards to personnel
Ϙ Functional disturbances or damaged
instruments
Danger due to hot surface.
This symbol warns against touching
accessible hot surfaces, e.g. those of
light bulbs.
Important information
This symbol indicates additional infor-
mation or explanations that intend to
provide clarity.
Explanatory notes
ϐ This symbol within the text points to additi-
onal information and explanations.
Instructions for disposing of the instrument, accessory components, and
consumables.
Leica DM750 Manual 7
Important Notes
Description
The Leica DM750 microscope meets today's
state of the art of technology. Nevertheless,
hazards may still arise during operation. The
potential risks are described below.
Before installing, operating or using the
instrument, it is mandatory to read this
user manual. In particular, please observe all
safety instructions.
Contact address
Leica Microsystems (Schweiz) AG
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)
User Manual
This user manual includes important instructions related to operating safety, maintenance
and accessories,
Your Leica DM750 microscope comes
with an interactive CD-ROM with all
relevant user manuals. Keep it in a safe place,
and readily accessible to the user. User manuals and updates are also available for you
to download and print from our website at
www.leica-microsystems.com.
Accessories from third-party suppliers
You can combine individual system articles with
articles from external suppliers. Please read the
user manual and the safety requirements of the
supplier.
Original condition
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Legal requirements
Adhere to general and local regulations relating to accident prevention and environmental
protection.
EC Declaration of Conformity
Electrically operated accessories are constructed based on the state of the art of technology
and are provided with an EC Declaration of
Conformity.
Leica DM750 Manual 8
Intended Purpose of the Microscope
The Leica DM750 microscope, for which
this instruction manual has been written, is intended for biological routine and
research applications. This includes examining specimens taken from the human body
for the purpose of gaining information about
physiological or pathological conditions
or inborn anomalies, or testing for safety
and compatibility for potential recipients,
or for monitoring therapeutic measures.
The above-named microscope complies with
the Council Directive 98/79/EEC concerning in
vitro diagnostics.
Caution! The Leica DM750 microscope
is not intended for use with biologically
hazardous materials.
Caution! The manufacturer assumes
no liability for any noncompliant use
or use outside of the specifications from Leica
Microsystems CMS GmbH as well as any risks
that may arise from such use. In such cases, the
Declaration of Conformity shall be invalid.
Caution! This (IVD) instrument is not
intended for use in a patient environment as defined by DIN VDE 0100-710.
It is also not intended to be combined with
medical devices as defined by EN 60601-1. If a
microscope is electrically connected to a medical instrument in accordance with EN 60601-1,
the requirements defined in EN 60601-1-1 shall
apply. Not suitable for examining potentially
infectious specimens.
Leica DM750 Manual 9
Instructions on use
The Leica DM750 microscope may be
used only in closed rooms and must be placed
on a solid substrate.
The Leica DM750 microscope can be used in
clean rooms without any problems.
Always position the Leica DM750
microscope so that you can disconnect
it from the power supply at any time. The power
cable must remain accessible at all times,
because it is intended as a power disconnect
device.
Place of use
Only use the instruments in closed, dust free
rooms and between +10°C and +40°C. Protect
the devices from oil, chemicals and extreme
humidity. If using the devices outdoors, protect
them from dust and moisture. Never use electrical devices outdoors. Install electrical devices at
least 10 cm from the wall and away from flammable substances.
Avoid large temperature fluctuations, direct
sunlight and vibrations. These conditions
can distort measurements and micrographic
images.
In warm and warm-damp climatic zones,
the individual components require
special care in order to prevent the build-up of
fungus.
Non-intended use
Never install any other plug (NEMA
5-15P) or unscrew any mechanical
components unless expressly instructed to do
so in the instructions.
The devices and accessories described in
this instruction manual have been tested
for safety and potential hazards.
Leica DM750 Manual 10
Instructions on use (continued)
The responsible Leica affiliate must be
consulted whenever the instrument is
altered, modified or used in conjunction with
non-Leica components that are outside of the
scope of this manual!
Unauthorized alterations to the instru-
ment or noncompliant use shall void all
rights to any warranty claims.
Transport
If at all possible, use the original packaging for
shipping or transporting individual modules.
In order to prevent damage from vibrations,
disassemble all moving parts that (according
to the user manual) can be assembled and
disassembled by the customer and pack them
separately.
Disposal
Once the product has reached the end of its
service life, please contact Leica Service or Sales
about disposal.
Please observe and ensure compliance with the
national laws and regulations that implement,
for example, the EC Directive WEEE.
Like all electronic devices, this inst-
rument, its accessory components
and consumables must never be disposed of
with general household waste. Disposal must
comply with locally applicable laws and regulations.
Integration in third-party products
When installing Leica products into third-party
products, the manufacturer of the complete
system or its dealer is responsible for following
all applicable safety instructions, laws and
guidelines.
Leica DM750 Manual 11
Health risks and dangers of use
Health risks
Workplaces with microscopes facilitate
and improve the viewing task, but they
also impose high demands on the eyes and
holding muscles of the user. Depending on the
duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
this reason, appropriate measures for reduction
of the workload must be taken:
Ϙ Optimum workplace layout
Ϙ Frequent changes of activity
Ϙ Thorough training of the personnel, giving
consideration to ergonomic and organizational aspects
The ergonomic design and construction of the
Leica microscopy series are intended to reduce
the exertion of the user to a minimum.
Danger of infection
Direct contact with eyepieces is a
potential transmission method for
bacterial and viral infections of the eye.
The risk can be kept to a minimum by
using personal eyepieces for each indivi-
dual or detachable eyecups.
Dangers during use
Ϙ The Leica DM750 microscope may only be
connected to a grounded socket.
Ϙ The Leica DM750 microscope may not be
operated unless it is in proper functioning
condition.
LED Klasse 1 /LED 1 Laser Product
The illumination meets laser class 1 requirements in accordance with IEC 60825-1 "Safety
of Laser devices".
Caution: Hazardous radiation may occur if
controls, adjustment jigs, or procedures other
than those outlined in this User Manual are
used!
Class 1
LED Product
LED class 1
Leica DM750 Manual 12
Information for the person responsible for the instrument
Information for the person responsible for
the instrument
Ϙ Ensure that the Leica DM750 microscope is
used only by qualified personnel.
Ϙ Ensure that this user manual is always
available at the Leica DM750 microscope.
Ϙ Carry out regular inspections to make
certain that the authorized users are
adhering to safety requirements.
Ϙ When instructing new users, do so
thoroughly and explain the meanings of
the warning signs and messages.
Ϙ Assign individual responsibilities for star-
ting, operating and servicing the instrument and monitor the observance of these
responsibilities.
Ϙ Do not use the Leica DM750 microscope
unless it is in perfect condition.
Ϙ Inform your Leica representative or Leica
Microsystems (Schweiz) AG, Industry
Division, 9435 Heerbrugg, Switzerland,
immediately of any product defect that
could potentially cause injury or harm.
Ϙ If you use accessories made by third-party
manufacturers with the Leica DM750
microscope, be sure that each such manufacturer confirms the safety-engineering,
harmless usability of the product and
observe the product's user manual.
Ϙ Modifications and maintenance of the Leica
DM750 microscope may only be performed
by professionals expressly authorized by
Leica.
Ϙ Only original Leica replacement parts may
be used in servicing the product.
Ϙ After service work or technical modifica-
tions, the unit must be reconfigured with
observance to our technical requirements.
Ϙ If the unit is modified or serviced by
unauthorized persons, is improperly
maintained (as long as maintenance was
not carried out by us), or is handled improperly, Leica will not accept any liability.
Ϙ The electric installation in the building
must conform to the national standard, e.g.
current-operated ground leakage protection (fault-current protection) is suggested.
Leica DM750 Manual 13
Care instructions
General instructions
Ϙ Protect the Leica DM750 microscope
against damp, vapors, acids, alkalis, and
corrosive substances. Do not store chemicals in the vicinity.
Ϙ Protect the Leica DM750 microscope from
oil and grease. Never grease or oil mechanical parts or sliding surfaces.
Ϙ Follow the instructions of the disinfectant
manufacturer.
Ϙ It is advisable to enter a service agreement
with Leica Service.
Cleaning coated parts and plastic parts
Ϙ Dust and dirt particles should be removed
with a soft brush or lint-free cotton cloth.
Ϙ Remove coarse debris with a moistened
disposable cloth.
Ϙ Clinging dirt can be removed using petro-
leum ether or alcohol
Ϙ Acetone, xylene or nitro-containing thin-
ners must NOT be used.
Ϙ Never use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces, and
specimens could be contaminated by abraded particles.
Cleaning glass surfaces
Ϙ Remove dust using a dry and grease-free
brush made from hair, by blowing with
bellows, or by using a vacuum.
Ϙ Remove dirt using a clean cloth that has
been moistened with distilled water.
Ϙ Remove tough dirt using alcohol or petro-
leum ether.
Leica DM750 Manual 14
Accessories, maintenance and repair
Accessories
Only the following accessories may be used
with the Leica DM750 microscope:
Ϙ The Leica accessories described in this user
manual.
Ϙ Other accessories, provided that these have
been expressly approved by Leica as being
technically safe in this context.
Maintenance
Ϙ The Leica DM750 microscope is basically
maintenance-free. To ensure that it always
operates safely and reliably, we recommend
that you take the precaution of contacting
the responsible service organization.
You can arrange for periodic inspections
or, if appropriate, conclude a mainte-
nance contract with them.
Ϙ It is advisable to enter a service agreement
with Leica Service.
Ϙ For maintenance and repair, only OEM
spare parts may be used.
Repairs and service work
Ϙ Only original Leica Microsystems spare
parts may be used.
Ϙ Before opening the instruments, switch off
the power and unplug the power cable.
Ϙ Avoid contact with powered electrical
circuits, which can lead to injury.
Service address
In case of problems, please contact us as
follows:
Leica Microsystems (Schweiz) AG
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)
Phone +41 71 726 33 28
Leica DM750 Manual 15
Electrical data and ambient conditions
Fuse Replacement
Unplug the instrument before changing any fuses. The Leica
DM750 has two fuses, which are located behind the power cord
receptacle.
Only use the following fuse types: 5×20 mm, 1 A/250V, fast-acting
fuse (# 13RFAG30003)
Electrical data
Input: 100-240 V, 50/60 Hz, 5 W (3 W LED)
General safety notes
This instrument of safety class 1 has been built and tested in accordance
with the following safety requirements for electrical equipment for measurement, control, and laboratory use:
EN 61010-1: 2002-08
EN 61010-2-101: 2008-06
IEC 61010-1: 2010-06
EN 61326-1: 2006-10
EN 61326-2-6: 2006-10
EN 55011: 2007+A2: 2010-05
EN 60825-1: 2008-05
IEC 60825-1: 2007-03
LED Class 1
In order to maintain this condition and to ensure safe operation,
the user must follow the instructions and warnings contained in
this instruction manual.
Environment
Temperature for use +10 °C … +40 °C
Storage temperature -20 °C … +52 °C
+50 °F to +104 °F
Manipulation shock 25 mm on 50 mm hard wood
Transport shock (unpacked) 100 g / 6 ms
Transport shock (packed) 800 mm free fall
Transport vibrations (unpacked) 5–200 Hz / 1.5 g
Air pressure during use and storage: 500–1060 mbar
Humidity during use and storage: 20–90%
Installation Category II (Overvoltage Category)
Pollution degree 2
Leica DM750 Manual 16
The Leica DM750
Leica DM750 Manual 17
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