Leica DM5500 B, DM6000 B, DM6000 M User Manual [en, de, fr]
Leica DM5500 B
Leica DM6000 B
Leica DM6000 M
Operating Manual
Bedienungsanleitung
Manuel d’utilisation
1
Published September 2007 by:
Herausgegeben September 2007 von:
Publié en septembre 2007 par :
Leica Microsystems CMS GmbH
Ernst-Leitz-Straße
D-35578 Wetzlar (Germany)
Responsible for contents:
Verantwortlich für den Inhalt:
Responsable du contenu :
Dr. Jasna Roeth, Stefan Motyka
(Product manager „Life Science Research“)
(Produktmanager „Life Science Research“)
(Chef de produit „Life Science Research“)
Holger Grasse
(Safety Officer according to MPG §30)
(Sicherheitsbeauftragter nach MPG §30)
(Officier de sécurité selon MPG §30)
In case of questions, please contact the hotline:
Bei Fragen wenden Sie sich bitte an die Hotline:
Pour toute question, contacter l’assistance en ligne: Phone +49(0)64 41-2922 86
Fax +49(0)64 41-2922 55
E-Mail: MQM-Hotline@leica-microsystems.com
2
Leica DM5500 B
Leica DM6000 B
Leica DM6000 M
Operating Manual
3
Copyrights
Copyrights
All rights to this documentation are held by
Leica Microsystems CMS GmbH. Reproduction
of text or illustrations (in whole or in part) by
print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica
Microsystems CMS GmbH.
The term "Windows" can be used in the following text without further identification. It is a registered trademark of the Microsoft Corporation.
Otherwise, no inference with regard to the free
usability of product names may be drawn from
the use of those names.
The instructions contained in the following
documentation reflect state-of-the-art technology and knowledge standards. We have compiled the texts and illustrations as accurately as
possible. Nevertheless, no liability of any kind
may be assumed for the accuracy of this manual’s contents. Still, we are always grateful for
comments and suggestions regarding potential
mistakes within this documentation.
The information in this manual is subject to modification at any time and without notification.
4
Contents
Contents
1. Important Notes about this Manual ...... 7
2. Intended Purpose of Microscopes ........ 8
3. Safety Notes ............................................... 9
3.1 General Safety Notes ............................... 9
3.2 Electrical Safety ........................................ 10
3.3 Disposal ....................................................... 11
4. Overview of the Instrument .................... 12
5. Unpacking the Microscope .................... 18
6. Assembling the Microscope .................. 20
6.1 Stage ............................................................ 21
6.2 Condenser ................................................... 22
6.3 Tube and Eyepieces .................................. 23
6.4 Objectives ................................................... 24
6.5 Light Sources for the
Transmitted Light Axis.............................. 24
6.6 Light Sources for the
Incident Light Axis..................................... 26
6.6.1 106 z lamp housing ......................... 26
6.6.2 External light source EL6000 ........ 31
6.7 Equipping the
Incident Light Turret Disc ........................ 32
6.8 Polarizer and Analyzer ............................. 33
6.9 DIC Prisms .................................................. 34
6.10 Optional Accessories ............................... 35
6.11 Connecting the Leica CTR5500/CTR6000
Electronics Box.......................................... 37
6.12 Connecting the Computer ........................ 38
6.13 Connection to the Power Supply ............ 38
7. Startup ......................................................... 39
7.1 Functional Principle .................................. 39
7.2 Switching on ............................................... 46
7.3 The Leica SmartTouch.............................. 47
7.4 The Function Keys at the stand .............. 48
7.5 The Remote Control Element
SmartMove ................................................. 49
7.6 Köhler Illumination .................................... 49
7.6.1 Transmitted Light ............................ 49
7.6.2 Incident Light................................... 51
7.7 Checking Phase Contrast Rings ............. 52
7.8 Adjusting Motorized Polarizer ................ 54
7.9 Adjusting the Light Sources .................... 54
8. Operation .................................................... 60
8.1 Switching on ............................................... 60
8.2 Stages and Specimen Displacement .... 62
8.2.1 Manual stage (DM5500 B) ............ 62
8.2.2 Motorized stage (DM6000 B/M)... 63
8.3 Focusing ...................................................... 64
8.4 Tubes ........................................................... 65
8.5 Eyepieces .................................................... 67
8.6 Objectives ................................................... 67
8.7 Magnification Changer ............................. 70
8.8 Light Sources ............................................. 70
8.9 Aperture Diaphragm and
Field Diaphragm ......................................... 71
5
Contents
9. Contrasting Methods for
Leica DM5500 B and DM6000 B ............. 72
9.1 Transmitted Light....................................... 72
9.1.1 Bright Field....................................... 72
9.1.2 Phase Contrast................................ 73
9.1.3 Dark Field ......................................... 73
9.1.4 Polarization ...................................... 74
9.1.5 Differential Interference Contrast .. 75
9.2 Fluorescence .............................................. 76
9.3 Combi Mode ............................................... 77
10. Contrasting Methods for
Leica DM6000 M ........................................ 78
10.1 Incident Light ............................................. 78
10.1.1Bright Field ....................................... 78
10.1.2Dark Field ......................................... 78
10.1.3Polarization ...................................... 79
10.1.4Interference Contrast .................... 80
10.1.5Fluorescence ................................... 80
10.2 Transmitted Light ....................................... 81
10.2.1Bright Field ....................................... 81
10.2.2Polarization ...................................... 81
11. Trouble Shooting ....................................... 82
12. Care of the Microscope ........................... 85
12.1 Dust Cover .................................................. 85
12.2 Cleaning....................................................... 85
12.3 Handling Acids and Bases ...................... 86
13. Essential Wear and Spare Parts ............ 87
14. Abbreviations and Pictograms .............. 88
15. Index ............................................................ 90
16. EU Declaration of Conformity ................. 91
6
1. Important Notes about this Manual
1. Important Notes about this Manual
Caution!
This operating manual is an essential component of the microscope, and must be read
carefully before the microscope is assembled, put into operation or used.
Text symbols, pictograms and their meanings:
(1.2)
→
p.20
!
This operating manual contains important instructions and information for the operational
safety and maintenance of the microscope and
accessories. Therefore, it must be kept and
taken care of.
For the operation of the LeicaScreen and the
Leica Application Suite (LAS), please see
separate operating manual.
Numbers in parentheses, such as "(1.2)", correspond to illustrations (in the example, Figure 1,
Item 2).
Numbers with pointer arrows (for example
→ p.20), point to a certain page of this manual.
Caution!
Special safety instructions within this
manual are indicated with the triangle symbol shown here, and have a gray background.
Caution! The microscope and accessories can
be damaged when operated incorrectly.
Notes on how to dispose of the microscope,
its components and expendables.
Explanatory note.
Item not contained in all configurations
*
(optional).
7
2. Intended Purpose of Microscopes
2. Intended Purpose of Microscopes
The Leica DM5500 B and DM6000 B microscopes, to which this user manual belongs, are
designed for biological routine and research
applications. This includes the examination of
samples taken from the human body with a view
to providing information on physiological or
pathological states or congenital abnormalities,
or to determining the safety and compatibility
with potential recipients, or to monitoring therapeutic measures.
The Leica DM6000 M microscope is designed for
examinations in the field of materials research.
All the above-named microscopes comply with
the Council Directive 98/79/EEC concerning in
vitro diagnostics. They also conform to the
Council Directives 73/23/EEC concerning electrical apparatus and 89/336/EEC concerning electromagnetic compatibility for use in an industrial
environment.
The manufacturer assumes no liability for
damage caused by, or any risks arising from
using the microscopes for other purposes
than those for which they are intended or
not using them within the specifications of
Leica Microsystems CMS GmbH.
In such cases the conformity declaration
shall cease to be valid.
These (IVD) devices are not intended for use
in the patient environment defined by DIN
VDE 0100-710. Neither are they intended for
combining with medical devices according
to EN 60601-1. If a microscope is electrically
connected to a medical device according to
EN 60601-1, the requirements defined in
EN 60601-1-1 shall apply.
Caution!
Caution!
8
3. Safety Notes
3.1 General Safety Notes
This safety class 1 device is constructed and
tested in accordance with
EN 61010-2-101:2002,
EN 61010-1:2001,
IEC 1010-1:2001,
safety regulations for electrical measuring, control, and laboratory devices.
Caution!
In order to maintain this condition and to ensure safe operation, the user must follow the
instructions and warnings contained in this
operating manual.
3. Safety Notes
Caution!
The devices and accessories described in
this operating manual have been tested for
safety and potential hazards.
The responsible Leica affiliate or the main
plant in Wetzlar must be consulted whenever the device is altered, modified or used
in conjunction with non-Leica components
that are outside of the scope of this manual.
Unauthorized alterations to the device or
noncompliant use shall void all rights to any
warranty claims!
9
3. Safety Notes
3.2 Electrical Safety
General specifications
Leica CTR5500 and CTR6000 electronics box
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
90-250 V~
50-60 Hz
max. 290 VA
T6,3 A
(IEC 60127-2/3)
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
15-35°C
max. 80% to 30°C
II
2
Microscope
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
90-250 V~
50-60 Hz
See CTR5500/6000
See CTR5500/6000
15-35°C
max. 80% to 30°C
II
2
Supply unit ebq 100*
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
90-250 V~
50-60 Hz
max. 155 VA
2xT2A (IEC 127)
10-36°C
max. 80% to 30°C
II
2
(see enclosed manual)
Leica EL6000
For indoor use only.
Supply voltage:
Frequency:
Power consumption:
Fuses:
100-240 V~ (±10%)
50-60 Hz
max. 210 VA
5x20, 2.5 A, slow,
breaking capacity H
→ EL6000 manual
Ambient temperature:
Relative humidity:
0°-40°C
10-90%
non-condensing
Overvoltage category:
Contamination class:
II
2
(see enclosed manual)
Caution!
The power plug may only be plugged into an
outlet equipped with a grounding contact.
Do not interfere with the grounding function
by using an extension cord without a ground
wire. Any interruption of the ground wire inside or outside of the device, or release of
the ground wire connection, can cause the
device to become hazardous. Intentional
ground interruption is not permitted!
10
3. Safety Notes
Caution!
Through activating to the grounding connection (earth screw on the back of the Leica
CTR5500 and CTR6000 Electronics Box)
ancillary equipment with its own and/or
extra power supply may be brought to the
same ground wire potential. For connections
without a ground connector, Leica Service
must be consulted.
Caution!
Never use any fuses as replacements other
than those of the types and the current ratings listed here. Using patched fuses or
bridging the fuse holder is not permitted.
Caution!
Caution!
Before exchanging the fuses or lamps, be
absolutely certain to switch-off the main
power switch and remove the power cable.
Caution!
Touch the touchscreen using your finger
only. Never use a pen or other hard, sharp or
pointed objects.
3.3 Disposal
After the end of the product’s life, please
contact Leica Service or Leica Sales on how to
dispose of it.
Please observe the national laws and
ordinances which, for example, implement and
ensure compliance with EU directive WEEE.
The microscope’s electrical accessory components are not protected against water.
Water can cause electric shock.
Caution!
Protect the microscope from excessive temperature fluctuations. Such fluctuations can
lead to the accumulation of condensation,
which can damage the electrical and optical
components.
Ambient temperature: 15-35°C.
n.b.:
Like all electronic instruments, the
microscope, its components and expendables may not be disposed of as general
household waste!
11
4. Overview of the Instrument
4. Overview of the Instrument
Specification
Contrasting Method
Transmitted Light Axis
Incident Light Axis
Leica DM5500 B/DM6000 B
• transmitted light:
BF, DF, PH, ICT (DIC), Pol
• incident light: Fluo
• automatic Illumination Manager
(automatic aperture diaphragm and field diaphragm,
automatic intensity control)
• automatic Constant Color Intensity Control (CCIC)
• motorized shutter
• integrated into the stand
• motorized 8x filter turret
( 5x optional)
• with FIM (Fluorescence Intensity Manager) for decreasing light intensity in 5
steps
• mechanical “Booster Lens“
for increasing fluorescence
intensity
• motorized Excitation Manager to control the fluorescence emission when using
double and triple filter cubes
(optional).
• ultra-fast, internal filter
wheel (IFW), motorized
(optional)
• motorized shutter
(speed < 0.1 s)
Leica DM6000 M
• transmitted light: BF, Pol
• incident light:
BF, DF, ICR (DIC), Pol, Fluo
• integrated into the stand
• motorized 4x filter turret
(2 fixed positions, 2 variable
positions)
• automatic Illumination
Manager
• motorized shutter
(speed < 0.1 s)
12
Tube
• motorized or manual
• up to 3 beam splitting positions
• optionally with two camera outputs
(for one digital and e.g. one analog camera)
4. Overview of the Instrument
Specification
Magnification Changer
(optional)
Objective Turret
X/Y Stage
Condenser
Leica DM5500 B/DM6000 B
• manual
• absolute coded
• magnification steps:
1x; 1.25x; 1.6x
• DM6000 B: motorized
DM5500 B: manual
• absolute coded
• 7x for objectives with M25
thread
• mot. DIC objective prism turret with 4 positions
(optional)
• DM6000 B/M: motorized, direct stepper motor drive
DM5500 B: manual
• replaceable specimen stage
• zero position defined by end switch
• outer dimensions: 234 mm x 157 mm
• travel range: 76 mm x 50 mm
• smallest increment: 0.3 µm
• motorized condenser head
• automatic change between contrasting methods:
motorized condenser turret for light rings, DF stop,
DIC prisms
• optional polarizer integrated and motorized
• automatic Köhler Illumination
Leica DM6000 M
• manual
• absolute coded
• magnification steps:
1x; 1.5x; 2x
• motorized
• absolute coded
• 6x for objectives with M32
thread
• mot. DIC objective prism
turret with 4 positions
(optional)
Z Drive
• motorized
• dovetail for exchanging stage
• travel range: 25 mm
• smallest increment: 0.015µm
• max. speed: 5 mm/s
• min. speed: 1 mm/s
• max. load: 4 kg
13
4. Overview of the Instrument
Specification
Control Panels
Leica CTR5500
Leica CTR6000
Electronics Box
Computer Interface
Software Tools
Leica DM5500 B/DM6000 B Leica DM6000 M
• Function keys at the stand
• additional variable function keys
• focusing knobs
• Leica SmartTouch: touch sensitive LC display
• SmartMove: ergonomic control element
for x,y,z control with additional variable function keys
• Leica STP6000: ergonomic control element for x,y,z
control with 11 additional variable function keys
and touch sensitive LC display
• separate control unit to control
• z-drive
• xy-stage (CTR6000)
• objective turret (CTR6000)
with:
• supply voltage for 100W halogen lamp
• supply voltage for SmartMove
See → p.10 (electrical safety))
• USB 2.0
• Leica Application Suite (LAS)
TM
for Windows
2000, XP, Vista
• for:
• microscope and camera configuration
• microscope and camera control
• image recording and image storing
14
4. Overview of the Instrument
2
3
Fig. 1 System overview DM6000 B
4
5
6 7
81
Abb. 1 Control elements of the Leica STP6000
1 Touchscreen
2 Information key
3,4 Variable function keys, user-programmable
5 Fine focus adjustment
6 Coarse focus adjustment
7 Movement in Y direction
8 Movement in X direction
15
4. Overview of the Instrument
14
1
2
3
4
5
6
7
13
Fig. 2 Left side of the stand Leica DM6000 B
1 Eyepiece
2 Eyepiece tube
3 Motorized tube MBDT
4 Motorized objective nosepiece with objectives
5 Motorized specimen stage with specimen holder
6 Condenser
7 Leica SmartTouch
9101112
8 Function keys field diaphragm
9 Transmitted light/incident light switch
10 Function keys aperture diaphragm
11 Function keys light intensity
12 Focus wheel
13 Variable function keys (factory pre-assigned)
14 Lamp adjustment window
n.b.: Illustrations for Leica DM5500 B similar, but with manual objective nosepiece and manual
specimen stage
16
21
4. Overview of the Instrument
15
23
22
16
Fig. 3 Right side of the stand Leica DM6000 B
15 Lamp housing for incident light
16 Lamp housing for transmitted light
17 Transmitted light filter, optional
18 Transmitted light filter, optional
19 Variable function keys (factory pre-assigned)
20 Focus wheel
21 DIC turret
22 Motorized filter turret
23 Magnification changer
19
18 17
3
20
Fig. 4 Remote control element SmartMove
1 Movement (x-direction)
2 Movement (y-direction)
3 Focus adjustment
4 Variable function keys
(factory pre-assigned)
4
1
2
17
5. Unpacking the Microscope
5. Unpacking the Microscope
The device is delivered in several boxes.
The stand box contains the following compo-
nents:
• Stand with integrated incident light axis and
objective nosepiece
• Specimen stage with stage bracket
• Power cable and PC connecting cable
• CD with software package Leica Application
Suite (LAS)
• Instructions and list of microscope default
settings („Identification Sheet“)
The system box contains the microscope acces-
sories:
• Tube
• Eyepieces
The Leica CTR5500 or CTR6000 Electronics Box,
the remote control element SmartMove, the
external ebq 100 supply unit* and the external
EL6000 compact light source* are delivered in
separate packaging.
First, carefully remove all components from the
transportation and packaging materials.
Note:
If at all possible, avoid touching the lens surfaces of the objectives. If fingerprints do appear
on the glass surfaces, remove them with a soft
leather or linen cloth. Even small traces of finger
perspiration can damage the surfaces in a short
time. See the chapter, "Care of the Microscope"
p. 85, for additional instructions.
→
Caution!
• Objectives
• Condenser
• Lamp housings with accessories
• Fitting tool
• Depending on configuration, additional microscope accessories such as filter cubes, etc.
18
Do not yet connect the microscope and peripherals to the power supply at this point!
5. Unpacking the Microscope
Installation location
Work with the microscope should be performed
in a dust-free room, which is free of oil vapors
and other chemical vapors, as well as extreme
humidity. At the workplace, large temperature
fluctuations, direct sunlight and vibrations
should be avoided. These conditions can distort
measurements and micrographic images.
Allowable ambient conditions
Temperature 15-35°C
Relative humidity maximum 80% up to 30°C
Microscopes in warm and warm-damp climatic
zones require special care in order to prevent
the build up of fungus.
See the chapter, "Care of the Microscope" →
for additional instructions.
Caution!
Electrical components must be placed at least
10 cm from the wall and away from flammable substances.
p. 85,
Transport
For shipping or transporting the microscope
and its accessory components, the original
packaging should be used.
As a precaution to prevent damage from vibrations, the following components should be disassembled and packaged separately:
• Unscrew the objectives.
• Remove the condenser.
• Remove the stage.
• Remove the lamp housings.
• Disassemble the burner of 106 z lamp housing.
• Remove all moving or loose parts.
19
6. Assembly
6. Assembling the Microscope
The microscope components are logically assembled in this order:
• Stage
• Condenser
• Tube
• Eyepieces
• Objectives
• Light sources
• Filter cubes/reflectors*
Only a few commonly used screwdrivers and
keys are necessary for assembly, which are included in the delivery package.
When using intermediate systems and optical
accessories, the sequence may vary.
In this case, read Chapter
"6.10 Optional accessories" → p. 35.
20
6.1 Stage
Caution:
!
Before assembling the stage, make sure no objectives are installed!
6. Assembly
Note:
For thicker specimens (Leica DM6000 M) the
stage can be set to a correspondingly lower
level.
• Place the specimen holder on the stage and
fasten it with the two screws (5.1).
• Using the condenser height adjuster (5.2), turn
the condenser holder completely upwards, i.e.
as close to the stage as possible.
• Loosen the stage clamp (5.3) slightly.
• From above, set the stage clamp onto the
dovetail guide (6.2) and push the stage downwards until the upper end of the dovetail guide
is tightly fastened to the upper end of the
stage clamp.
• Firmly tighten the stage clamp (6.1).
Fig. 5 Object stage (motorized)
1 Locking screws for specimen holder
2 Condenser height adjuster
3 Stage clamp
1
Caution:
!
This changes the pre-set focus position and the
lower threshold. Both positions have to be set
again. See 8.3. Focusing, → p. 64.
For the motorized stage only:
•
Connect the stage cable to the electronics
box Leica CTR6000.
See chapter 6.11 → p. 37.
Fig. 6 Assembling the stage
1 Stage clamp
2 Dovetail guide
23
1
2
21
6. Assembly
6.2 Condenser
• Screw the condenser head into the condenser.
• Using the condenser height adjuster (7.4), turn
the condenser holder (7.1) completely downwards.
• Unscrew the clamping screw for the condenser (7.3) far enough so that the condenser
can be inserted from the front.
• From the front, insert the condenser into the
condenser holder as far as it will go. On the
underside of the condenser, there is an orientation pin (8.1), which must be located in the
guiding notch (9.1).
• Pull the condenser’s clamping screw (7.3) so
that the condenser is locked in place.
• Connect the condenser by connecting the
condenser cable (10.1) with the stand. The
black index point on the stand points to the
groove of the plug.
Note:
The condenser must be centered before using
the microscope.
Köhler illumination p. 49.
→
Fig. 8
Underside of condenser
1 Orientation pin
Fig. 9 Condenser holder
1 Guiding notch
1
1
Fig. 7 Condenser holder
1 Condenser holder
2 Condenser centering
3 Clamping screw for condenser
4 Condenser height adjuster
1
2
3
22
Fig. 10 Condenser connector
1 Condenser cable socket
1
4
6. Assembly
6.3 Tube and Eyepieces
The tube is mounted to the stand either directly or
with the use of intermediate modules. It is fastened
in place with the side clamping screw (12.1).
Only for the motorized tube MBDT:
•
Remove the transportation lock (11.1) from the
bottom side of the tube.
• Loosen the clamping screw (12.1). on the
stand.
• Insert the tube in the circular receptacle
(dovetail ring).
• Retighten the clamping screw (12.1).
Only for the motorized tube MBDT:
•
Connect the tube to the stand with the connector socket (13.1).
• The eyepieces are inserted into the eyepiece
tubes on the tube.
Fig. 11 Bottom side of the tube
1 Transportation lock
1
Fig. 12 Fastening the tube
1 Clamping screw
1
Note:
For eyepieces that are not included in shipment,
we recommend to learn them in with the
Software Leica Application Suite (LAS), module:
Set-Up. This ensures that the information about
total magnification on the LeicaScreen is
correct.
Fig. 13 Motorized tube connection
1 Connector socket
1
23
6. Assembly
6.4 Objectives
The receptacles on the objective turrets are
numbered (Fig. 14). The individual objectives
have already pre-assigned positions at the factory according to their configuration.
A list of the exact objective positions is provided
in shipment (“Identification Sheet”).
Caution:
!
Cover unoccupied threads on the turret with
dust protector caps!
Note:
We recommend to perform a parfocality adjustment with the Software Leica Application Suite
(LAS), module: Fine Tuning.
6.5 Light Sources for the Transmitted Light Axis
Caution!
Be sure that the lamp housing is disconnected from the power supply. Unplug the
power plug and the power supply during assembly.
Caution!
Light sources pose a potential irradiation
risk (glare, UV-radiation, IR-radiation).
Therefore, lamps have to be operated in
closed housings.
Fig. 15
Releasing the
fastening screw
at lamp housing 107/2
Fig. 14
Objective turret
with labeled
objective
receptacles
24
Fig. 16
Lamp housing 107/2,
opened
1 Mount with
halogen lamp
2 Collector
1
2
6. Assembly
107/2 Lamp Housing
This lamp housing is used with a 12V 100W halogen lamp, which is already mounted.
In case the lamp has to be removed:
• Remove the fastener screw on the housing
(Fig. 15).
• Remove the housing by pulling it upwards.
• Remove the lamp.
• Insert the new 12V 100W lamp (16.1) with the
dust cover straight into the socket until it
stops. Be sure that the lamp is inserted
straight.
• Remove the lamp’s dust cover.
Caution!
Do not remove the lamp’s dust cover until
you have installed the lamp. Avoid fingerprints on the lamp.
Fig. 17 Rear side of stand
1 Incident light lamp housing receptacle
2 Transmitted light lamp housing receptacle
3 12 V 100 W connection for transmitted light (symbol: )
4 12 V 100 W connection for incident light (symbol: )
5 Lamp power cable
1
5
2
Fig. 18 Rear side of Leica CTR6000
1 Connection for lamp power cable from the stand
34
• Replace the housing and fasten it in place using the fastening screw.
• Place the lamp housing in the transmitted light
lamp housing receptacle (17.2) and fasten it
with the clamping screw on the side.
• Connect the lamp housing to the power supply
for transmitted light (symbol:
) (17.3).
• Now connect the lamp power cable of the microscope (17.5) to the Leica CTR5500 or
CTR6000 Electronics Box (18.1).
1
25
6. Assembly
6.6 Light Sources for the Incident Light Axis
Caution!
Light sources pose a potential irradiation
risk (glare, UV-radiation, IR-radiation).
Therefore, lamps have to be operated in
closed housings.
During assembly, always unplug the power
supply unit of the 106 z lamp housing from its
socket.
During assembly work on xenon burners, always wear the supplied protective gloves
and face protection (Fig. 19) (risk of explosion).
Never touch the glass parts of the burner
with bare hands.
Never look directly into the beam path
(blinding hazard).
6.6.1 106 z lamp housing
This lamp housing is used with a 12V 100W halogen lamp or various gas discharge lamps.
Caution!
Make sure to follow the instructions and
safety notes of the lamp supplier.
Before changing lamps allow at least 30 mins
for cooling down!
Fig. 20 106 z lamp housing (on the side, open)
1 Cover raised
2 Collector
3 12 V 100 W lamp or
gas discharge lamp in mount
4 Reflector (mirror)
5, 6, 7 Adjusting screw for x-y reflector
8 Fastening screw for lamp mount
9 Socket for contact plug
Fig. 19
Protective gloves and mask
26
1
2
4
5
3
6
7
898
6. Assembly
Inserting the 12V 100W halogen lamp into the
106 z lamp housing
• Unscrew the fastening screws of the cover
and lift up the cover (20.1).
• Unscrew the fastening screws of the lamp
mount (20.8) and pull out the mount (Fig. 21).
• Insert the lamp with the dust cover straight
into the socket until it stops.
Caution!
Do not remove the lamp’s dust cover until
you have installed the lamp. Avoid fingerprints on the lamp.
• Remove the dust cover.
• Reinsert the lamp mount and retighten the
fastening screw (20.8).
• Close the lamp housing and retighten the fastening screws.
• Place the lamp housing in the incident light
lamp housing receptacle (22.1) and fasten it
with the clamping screw on the side.
• Connect the lamp housing to the power supply
for incident light (symbol
) (22.4).
• Now connect the lamp power cable of the microscope (22.5) to the Leica CTR5500 or
CTR6000 electronics box (18.1, p. 25)
Fig. 22 Rear side of stand
1 Incident light lamp housing receptacle
2 Transmitted light lamp housing receptacle
3 12 V 100 W connection for transmitted light (symbol: )
4 12 V 100 W connection for incident light (symbol: )
5 Lamp power cable
Fig. 21 Lamp mount with 12 V 100 W halogen lamp
1
5
2
34
27
6. Assembly
Inserting the gas discharge lamps (Hg and Xe)
into the 106z lamp housing
Hg and Xe lamps are powered by separate supply units.
Read the separate instruction manual provided
with these supply units.
The following gas discharge lamps may be used
and require different supply units and lamp
mounts (Fig. 23):
Type Typical bulb life*
50 W high-pressure mercury burner (alternating current) 100 hrs.
100 W high-pressure mercury burner (direct current) 200 hrs.
100 W high-pressure mercury burner (direct current, type 103 W/2) 300 hrs.
75 W High-pressure xenon burner (direct current) 400 hrs.
* Please regard the data sheets of the burners.
28
• To open the 106 z lamp housing, unscrew the
fastening screws on the cover.
6. Assembly
Caution!
• Remove the transport anchorage (red plastic
rod in place of the burner) in the lamp mount.
To do so, remove the lower clamp (23.1). Pull
up the cooling element (23.3) and turn it to the
side. Detach the lower clamp system (23.2)
and remove the transport anchorage.
• Install the burner in reverse order.
Fig. 23 a-c Lamp mounts for gas discharge lamps
1 Upper clamping system, 2 Lower clamping system, 3 Cooling element
4 Nipple of the mercury 50 burner, 5 Dust cover of the mercury 75 burner
Hg 50
1
4
a
3
2
Hg 50 burner:
After installation, the labeling must be upright.
If a glass melt nipple is present (23a.4), position it by turning the burner so that the nipple
does not come in the way of the beam path
later, but instead is positioned
Xe 75 burner:
Remove the burner’s dust cover (23b.5) after
you have installed the burner.
Xe 75
sideways.
b
3
1
5
2
Hg 100
1
2
c
3
29
6. Assembly
• Insert the lamp mount, with the burner installed, into the lamp housing and tighten it
with the screws (24.8).
• Put the lid down again. Plug in the contact
plug as far as it goes and retighten the
screws.
• Place the lamp housing in the incident light
lamp housing receptacle (25.1) and fasten it
with the clamping screw on the side.
• Connect the lamp housing to the power supply
(26.1).
Fig. 25 Rear side of stand
1 Incident light lamp housing receptacle
2 Transmitted light lamp housing receptacle
3 12 V 100 W connection for transmitted light (symbol: )
4 12 V 100 W connection for incident light (symbol: )
5 Lamp power cable
Fig. 24 106 z lamp housing (on the side, open)
1 Cover raised
2 Collector
3 12 V 100 W lamp or
gas discharge lamp in mount
4 Reflector (mirror)
5, 6, 7 Adjusting screw for x-y reflector
8 Fastening screw for lamp mount
9 Socket for contact plug
1
2
3
898
4
5
6
7
30
Fig. 26 Rear side of the ebq 100 supply unit
1 Lamp connection
1
1
5
2
34
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