Leica DM500 B User Manual

Leica DM500, DM500 B Manual
Chapter overview
Safety regulations 4
The Leica DM500 , DM500 B 15
Get Ready! 18
Get Set! 25
Go! 34
Care of the Microscope 36
Dimensions 39
Leica DM 500, DM500 B Manual 2
Contents
Safety regulations 4
Safety concept 5 Symbols used in this operating manual 6 Important Notes 7 Instructions on use 8 Health risks and dangers of use 10 Information for the person responsible for the instrument 11 Care instructions 12 Accessories, maintenance and repair 13 Electrical data and ambient conditions 14
The Leica DM500, DM500 B 15
Congratulations! 16 Unpacking 17
Get Ready! 18
Attaching the viewing Tubes 19 Leica EZ Viewing Tube – integrated eyepieces 20 Leica Standard Viewing Tube – separated eyepieces 22 Eyeguards 23 Installing Objectives and Substage Condenser 24
Get Set! 25
Turning on the microscope 26 Using the Condenser 27 Prepare to View a Specimen Slide 28 Focusing 29 Viewing Tube Adjustment 30 Oil Immersion Technique 32
Go! 34
Ready! Set! Go! 35
Care of the Microscope 36
General maintenance 37
Dimensions 39
Leica DM 500, DM500 B Manual 3

Safety regulations

Leica DM 500, DM500 B Manual 4

Safety concept

The individual modules of the Leica DM micros­copy series include an interactive CD-ROM with all relevant user manuals several languages. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site at www.leica-microsystems.com.
This operating manual describes the special functions of the individual modules of the Leica DM microscopy series and contains important instructions for their operational safety, main­tenance, and accessories.
The "Safety concept" booklet contains addi­tional safety information regarding the service work, requirements and the handling of micro­scope, accessories and electrical accessories as well as general safety instructions.
You can combine individual system articles with articles from external suppliers. Please read the user manual and the safety requirements of the supplier.
Before installing, operating or using the instru­ments, read the user manuals listed above. In particular, please observe all safety instruc­tions.
To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals.
Leica DM 500, DM500 B Manual 5

Symbols used in this operating manual

Warning of a danger
This symbol indicates especially impor-
•
tant information that must be read and
complied with.
Failure to comply can cause the following:
Ϙ Hazards to personnel
Ϙ Functional disturbances or damaged instru-
ments
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
Ϙ Hazards to personnel
Ϙ Functional disturbances or damaged
instruments
Danger due to hot surface.
This symbol warns against touching
accessible hot surfaces, e.g. those of
light bulbs.
For Model DM500 B (Battery Version)
Dispose of old batteries and defective
appliances in accordance with the stat-
utory regulations.
Important information
This symbol indicates additional infor-
mation or explanations that intend to
provide clarity.
Explanatory notes
ϐ This symbol within the text points to addi-
tional information and explanations.
Leica DM 500, DM500 B Manual 6

Important Notes

Description
The Leica DM500 and DM500 B microscope meets today's state of the art of technology. Neverthe­less, hazards may still arise during operation. The potential risks are described below.
Before installing, operating or using the
•
instruments, it is required to read this user manual. In particular, please observe all safety instructions.
Contact address
Leica Microsystems (Switzerland) Ltd. Industry Division Max Schmidheiny Strasse 201 CH-9435 Heerbrugg (Switzerland)
User Manual
This user manual includes important instruc­tions related to operating safety, maintenance and accessories,
Your Leica DM500 or DM500 B micro-
scope comes with an interactive CD-ROM with all relevant user manuals. Keep it in a safe place, and readily accessible to the user. User manuals and updates are also available for you to download and print from our web site at www.leica-microsystems.com
Accessories from third-party suppliers
You can combine individual system articles with articles from external suppliers. Please read the user manual and the safety requirements of the supplier.
Original condition
To maintain the unit in its original condition and to ensure safe operation, the user must follow the instructions and warnings contained in these user manuals.
Legal requirements
Adhere to general and local regulations relat­ing to accident prevention and environmental protection.
EC Declaration of Conformity
Electrically operated accessories are constructed based on the state of the art of technology and are provided with an EC Declaration of Confor­mity.
Leica DM 500, DM500 B Manual 7

Instructions on use

Intended use
The Leica DM500 and DM500 B microscopes are optical instrument which use magnification and illumination to improve the visibility of objects. It is used for observing and documenting.
The Leica DM500 and DM500 B microscopes may be used only in closed rooms and must be placed on a solid floor.
The Leica DM500 or DM500 B microscopes can be used in clean rooms without any problems.
Position the Leica DM500 or
•
DM500 B microscope so that it can be disconnected from the power supply at any time. The power cord must remain accessible at all times, because the power supply is foreseen as mains disconnecting device.
Place of use
Only use the instruments in closed, dust free rooms and between +10 °C and +40 °C. Protect the devices from oil, chemicals and extreme humidity. If using the devices outdoors, protect them from dust and moisture. Never use electri­cal devices outdoors. Install electrical devices at least 10 cm from the wall and away from flam­mable substances.
Avoid large temperature fluctuations, direct sunlight and vibrations. These conditions can distort measurements and micrographic images.
In warm and warm-damp climatic zones,
the individual components require special care in order to prevent the build-up of fungus.
Non-intended use
Using the instrument in any way
•
contrary to the specifications in the user manual can lead to bodily harm and damage to objects. Doing so can impair the functions of the protective measures provided.
Never install any other plug (NEMA
5-15P) or unscrew any mechanical components unless expressly instructed to do so in the instructions.
CAUTION: Risk of explosion if battery is
replaced by an incorrect type. Dispose of used batteries according to the instructions.
The devices and accessories described in
this operating manual have been tested
for safety and potential hazards.
Leica DM 500, DM500 B Manual 8
Instructions on use (continued)
The responsible Leica affiliate must be
•
consulted whenever the instrument is altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual.
Unauthorized alterations to the device
•
or noncompliant use shall void all rights to any warranty claims.
Transport
If at all possible, use the original packaging for shipping or transporting individual modules.
In order to prevent damage from vibrations, disassemble all moving parts that (according to the user manual) can be assembled and disas­sembled by the customer and pack them sepa­rately.
Disposal
Disposal must comply with locally applicable laws and regulations.
Integration in third-party products
When installing Leica products into third-party products, the manufacturer of the complete system or its dealer is responsible for follow­ing all applicable safety instructions, laws and guidelines.
Leica DM 500, DM500 B Manual 9

Health risks and dangers of use

Health risks
Workplaces with microscopes facilitate
and improve the viewing task, but they also impose high demands on the eyes and holding muscles of the user. Depending on the duration of uninterrupted work, asthenopia and musculoskeletal problems may occur. For this reason, appropriate measures for reduction of the workload must be taken:
Ϙ Optimum workplace layout
Ϙ Frequent changes of activity
Ϙ Thorough training of the personnel, giving
consideration to ergonomic and organiza­tional aspects
The ergonomic design and construction of the Leica microscopy series are intended to reduce the exertion of the user to a minimum.
Danger of infection
Direct contact with eyepieces is a
•
potential transmission method for viral
infections of the eye.
The risk can be kept to a minimum by
using personal eyepieces for each indi-
vidual or detachable eyecups.
Dangers during use
Ϙ The Leica DM500 or DM500 B microscope
may only be connected to a grounded socket.
Ϙ The Leica DM500 or DM500 B microscope
may not be operated unless it is in proper functioning condition.
LED Klasse 1 /Class 1 LED Product
The illumination meets laser class 1 require­ments in accordance with IEC 60825-1 "Safety of LED products".
Caution: Use of controls or adjustments or
performance of procedures other than those specified herein may result in hazardous radia­tion exposure!
Class 1
LED Product
LED class 1
Leica DM 500, DM500 B Manual 10

Information for the person responsible for the instrument

Information for the person responsible for the instrument
Ϙ Ensure that the Leica DM500 or DM500 B
microscope is used only by qualified person­nel.
Ϙ Ensure that this user manual is always avail-
able at the Leica DM500 or DM500 B micro­scope.
Ϙ Carry out regular inspections to make
certain that the authorized users are adher­ing to safety requirements.
Ϙ When instructing new users, do so thor-
oughly and explain the meanings of the warning signs and messages.
Ϙ Assign individual responsibilities for start-
ing, operating and servicing the instru­ment and monitor the observance of these responsibilities.
Ϙ Do not use the Leica DM500 or DM500 B
microscope unless it is in perfect condition.
Ϙ Inform your Leica representative or Leica
Microsystems (Schweiz) AG, Industry Divi­sion, 9435 Heerbrugg, Switzerland, imme­diately of any product defect that could potentially cause injury or harm.
Ϙ If you use accessories made by third-party
manufacturers with the Leica DM500 or DM500 B microscope, be sure that each such manufacturer confirms that the combination is safe to use and observe the product's user manual.
Ϙ Modifications and maintenance of the Leica
DM500 or DM500 B microscope may only be performed by professionals expressly authorized by Leica.
Ϙ Only original Leica replacement parts may
be used in servicing the product.
Ϙ After service work or technical modifica-
tions, the unit must be reconfigured with observance to our technical requirements.
Ϙ If the unit is modified or serviced by unau-
thorized persons, is improperly maintained (as long as maintenance was not carried out by us), or is handled improperly, Leica will not accept any liability.
Ϙ The electric installation in the building
must conform to the national standard, e.g. current-operated ground leakage protec­tion (fault-current protection) is suggested.
Leica DM 500, DM500 B Manual 11

Care instructions

General instructions
Ϙ Protect the Leica DM500 or DM500 B micro-
scope against damp, vapors, acids, alkalis, and corrosive substances. Do not store chemicals in the vicinity.
Ϙ Protect the Leica DM500 or DM500 B micro-
scope from oil and grease. Never grease or oil mechanical parts or sliding surfaces.
Ϙ Follow the instructions of the disinfectant
manufacturer.
Ϙ It is advisable to enter a service agreement
with Leica Service.
Cleaning coated parts and plastic parts
Ϙ Dust and dirt particles should be removed
with a soft brush or lint-free cotton cloth.
Ϙ Remove coarse debris with a moistened
disposable cloth.
Ϙ Clinging dirt can be removed using petro-
leum ether or alcohol
Ϙ Acetone, xylene or nitro-containing thin-
ners must NOT be used.
Ϙ Never use chemicals to clean colored
surfaces or accessories with rubberized parts. This could damage the surfaces, and specimens could be contaminated by abraded particles.
Cleaning glass surfaces
Ϙ Remove dust using a fine, dry and grease-
free brush made from hair, by blowing with a squeeze blower, or by using a vacuum.
Ϙ Remove dirt using a clean cloth that has
been moistened with distilled water.
Ϙ Remove tough dirt using alcohol, chloro-
form or petroleum ether.
Leica DM 500, DM500 B Manual 12

Accessories, maintenance and repair

Accessories
Only the following accessories may be used with the Leica DM500 or DM500 B microscope:
Ϙ The Leica accessories described in this user
manual.
Ϙ Other accessories, provided that these have
been expressly approved by Leica as being technically safe in this context.
Maintenance
Ϙ The Leica DM500 and DM500 B micro-
scopes are basically maintenance-free. To ensure that they always operate safely and reliably, we recommend that you take the precaution of contacting the responsible service organization.
You can arrange for periodic inspections
or, if appropriate, conclude a mainte-
nance contract with them.
Ϙ It is advisable to enter a service agreement
with Leica Service.
Ϙ For maintenance and repair, only OEM spare
parts may be used.
Repairs and service work
Ϙ Only original Leica Microsystems spare
parts may be used.
Ϙ Before opening the instruments, switch off
the power and unplug the power cable.
Ϙ Avoid contact with powered electrical
circuits, which can lead to injury.
Service address
In case of problems, please contact us as follows:
Leica Microsystems (Switzerland) Ltd. Industry Division Max Schmidheiny Strasse 201 CH-9435 Heerbrugg (Switzerland) Phone +41 71 726 33 28
Leica DM 500, DM500 B Manual 13

Electrical data and ambient conditions

Fuse Replacement
Unplug the instrument before changing any fuses. The
Leica DM500 has two fuses, which are located behind the
power cord receptacle.
Use only fuse type: 5×20 mm, 1 Amp/250V, Quick acting
•
(# 13RFAG30003)
Electrical data
DM500 Input: 100-240 VAC, 50/60 Hz, 5 W (3 W LED)
DM500 B Input: 100-240 VAC, 50/60 Hz, 0.5-0.2 A OR
4× 1.2 V AA rechargeable batteries
Use only 1.2 Volt NiMH type AA rechargeable batteries
•
with a maximum of 2700 mAh. Do not use non-recharge-
able AA batteries.
Environment
Temperature for use +10 °C … +40 °C
Storage temperature -20 °C … +52 °C
+50 °F … +104 °F
Manipulation shock 25 mm on 50 mm hard wood
Transport shock (unpacked) 100 g / 6 ms
Transport shock (packed) 800 mm free fall
Transport vibrations (unpacked) 5–200 Hz / 1.5 g
Atmospheric pressure "in use" and for storage: 500–1060 mBar
Humidity “in use” and for storage: 20–90%
Installation Category II (Overvoltage Category)
Pollution degree 2
Leica DM 500, DM500 B Manual 14
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