Safety concept 5
Symbols used in this operating manual 6
Important Notes 7
Instructions on use 8
Health risks and dangers of use 10
Information for the person responsible
for the instrument 11
Care instructions 12
Accessories, maintenance and repair 13
Electrical data and ambient conditions 14
The Leica DM500, DM500 B 15
Congratulations! 16
Unpacking 17
Get Ready! 18
Attaching the viewing Tubes 19
Leica EZ Viewing Tube –
integrated eyepieces 20
Leica Standard Viewing Tube –
separated eyepieces 22
Eyeguards 23
Installing Objectives and Substage Condenser
24
Get Set! 25
Turning on the microscope 26
Using the Condenser 27
Prepare to View a Specimen Slide 28
Focusing 29
Viewing Tube Adjustment 30
Oil Immersion Technique 32
Go! 34
Ready! Set! Go! 35
Care of the Microscope 36
General maintenance 37
Dimensions 39
Leica DM 500, DM500 B Manual 3
Safety regulations
Leica DM 500, DM500 B Manual 4
Safety concept
The individual modules of the Leica DM microscopy series include an interactive CD-ROM with
all relevant user manuals several languages.
Keep it in a safe place, and readily accessible
to the user. User manuals and updates are also
available for you to download and print from
our web site at
www.leica-microsystems.com.
This operating manual describes the special
functions of the individual modules of the Leica
DM microscopy series and contains important
instructions for their operational safety, maintenance, and accessories.
The "Safety concept" booklet contains additional safety information regarding the service
work, requirements and the handling of microscope, accessories and electrical accessories as
well as general safety instructions.
You can combine individual system articles with
articles from external suppliers. Please read the
user manual and the safety requirements of the
supplier.
Before installing, operating or using the instruments, read the user manuals listed above. In
particular, please observe all safety instructions.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Leica DM 500, DM500 B Manual 5
Symbols used in this operating manual
Warning of a danger
This symbol indicates especially impor-
•
tant information that must be read and
complied with.
Failure to comply can cause the following:
Ϙ Hazards to personnel
Ϙ Functional disturbances or damaged instru-
ments
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Failure to comply can cause the following:
Ϙ Hazards to personnel
Ϙ Functional disturbances or damaged
instruments
Danger due to hot surface.
This symbol warns against touching
accessible hot surfaces, e.g. those of
light bulbs.
For Model DM500 B (Battery Version)
Dispose of old batteries and defective
appliances in accordance with the stat-
utory regulations.
Important information
This symbol indicates additional infor-
mation or explanations that intend to
provide clarity.
Explanatory notes
ϐThis symbol within the text points to addi-
tional information and explanations.
Leica DM 500, DM500 B Manual 6
Important Notes
Description
The Leica DM500 and DM500 B microscope meets
today's state of the art of technology. Nevertheless, hazards may still arise during operation.
The potential risks are described below.
Before installing, operating or using the
•
instruments, it is required to read this
user manual. In particular, please observe all
safety instructions.
Contact address
Leica Microsystems (Switzerland) Ltd.
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)
User Manual
This user manual includes important instructions related to operating safety, maintenance
and accessories,
Your Leica DM500 or DM500 B micro-
scope comes with an interactive CD-ROM
with all relevant user manuals. Keep it in a safe
place, and readily accessible to the user. User
manuals and updates are also available for you
to download and print from our web site at
www.leica-microsystems.com
Accessories from third-party suppliers
You can combine individual system articles with
articles from external suppliers. Please read the
user manual and the safety requirements of the
supplier.
Original condition
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Legal requirements
Adhere to general and local regulations relating to accident prevention and environmental
protection.
EC Declaration of Conformity
Electrically operated accessories are constructed
based on the state of the art of technology and
are provided with an EC Declaration of Conformity.
Leica DM 500, DM500 B Manual 7
Instructions on use
Intended use
The Leica DM500 and DM500 B microscopes are
optical instrument which use magnification and
illumination to improve the visibility of objects.
It is used for observing and documenting.
The Leica DM500 and DM500 B microscopes
may be used only in closed rooms and must be
placed on a solid floor.
The Leica DM500 or DM500 B microscopes can
be used in clean rooms without any problems.
Position the Leica DM500 or
•
DM500 B microscope so that it can be
disconnected from the power supply at any
time. The power cord must remain accessible at
all times, because the power supply is foreseen
as mains disconnecting device.
Place of use
Only use the instruments in closed, dust free
rooms and between +10 °C and +40 °C. Protect
the devices from oil, chemicals and extreme
humidity. If using the devices outdoors, protect
them from dust and moisture. Never use electrical devices outdoors. Install electrical devices at
least 10 cm from the wall and away from flammable substances.
Avoid large temperature fluctuations, direct
sunlight and vibrations. These conditions
can distort measurements and micrographic
images.
In warm and warm-damp climatic zones,
the individual components require
special care in order to prevent the build-up of
fungus.
Non-intended use
Using the instrument in any way
•
contrary to the specifications in the user
manual can lead to bodily harm and damage to
objects. Doing so can impair the functions of
the protective measures provided.
Never install any other plug (NEMA
5-15P) or unscrew any mechanical
components unless expressly instructed to do
so in the instructions.
CAUTION: Risk of explosion if battery is
replaced by an incorrect type. Dispose
of used batteries according to the instructions.
The devices and accessories described in
this operating manual have been tested
for safety and potential hazards.
Leica DM 500, DM500 B Manual 8
Instructions on use (continued)
The responsible Leica affiliate must be
•
consulted whenever the instrument is
altered, modified or used in conjunction with
non-Leica components that are outside of the
scope of this manual.
Unauthorized alterations to the device
•
or noncompliant use shall void all rights
to any warranty claims.
Transport
If at all possible, use the original packaging for
shipping or transporting individual modules.
In order to prevent damage from vibrations,
disassemble all moving parts that (according to
the user manual) can be assembled and disassembled by the customer and pack them separately.
Disposal
Disposal must comply with locally applicable
laws and regulations.
Integration in third-party products
When installing Leica products into third-party
products, the manufacturer of the complete
system or its dealer is responsible for following all applicable safety instructions, laws and
guidelines.
Leica DM 500, DM500 B Manual 9
Health risks and dangers of use
Health risks
Workplaces with microscopes facilitate
and improve the viewing task, but they
also impose high demands on the eyes and
holding muscles of the user. Depending on the
duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
this reason, appropriate measures for reduction
of the workload must be taken:
Ϙ Optimum workplace layout
Ϙ Frequent changes of activity
Ϙ Thorough training of the personnel, giving
consideration to ergonomic and organizational aspects
The ergonomic design and construction of the
Leica microscopy series are intended to reduce
the exertion of the user to a minimum.
Danger of infection
Direct contact with eyepieces is a
•
potential transmission method for viral
infections of the eye.
The risk can be kept to a minimum by
using personal eyepieces for each indi-
vidual or detachable eyecups.
Dangers during use
Ϙ The Leica DM500 or DM500 B microscope
may only be connected to a grounded
socket.
Ϙ The Leica DM500 or DM500 B microscope
may not be operated unless it is in proper
functioning condition.
LED Klasse 1 /Class 1 LED Product
The illumination meets laser class 1 requirements in accordance with IEC 60825-1 "Safety
of LED products".
Caution: Use of controls or adjustments or
performance of procedures other than those
specified herein may result in hazardous radiation exposure!
Class 1
LED Product
LED class 1
Leica DM 500, DM500 B Manual 10
Information for the person responsible for the instrument
Information for the person responsible for
the instrument
Ϙ Ensure that the Leica DM500 or DM500 B
microscope is used only by qualified personnel.
Ϙ Ensure that this user manual is always avail-
able at the Leica DM500 or DM500 B microscope.
Ϙ Carry out regular inspections to make
certain that the authorized users are adhering to safety requirements.
Ϙ When instructing new users, do so thor-
oughly and explain the meanings of the
warning signs and messages.
Ϙ Assign individual responsibilities for start-
ing, operating and servicing the instrument and monitor the observance of these
responsibilities.
Ϙ Do not use the Leica DM500 or DM500 B
microscope unless it is in perfect condition.
Ϙ Inform your Leica representative or Leica
Microsystems (Schweiz) AG, Industry Division, 9435 Heerbrugg, Switzerland, immediately of any product defect that could
potentially cause injury or harm.
Ϙ If you use accessories made by third-party
manufacturers with the Leica DM500 or
DM500 B microscope, be sure that each
such manufacturer confirms that the
combination is safe to use and observe the
product's user manual.
Ϙ Modifications and maintenance of the Leica
DM500 or DM500 B microscope may only
be performed by professionals expressly
authorized by Leica.
Ϙ Only original Leica replacement parts may
be used in servicing the product.
Ϙ After service work or technical modifica-
tions, the unit must be reconfigured with
observance to our technical requirements.
Ϙ If the unit is modified or serviced by unau-
thorized persons, is improperly maintained
(as long as maintenance was not carried
out by us), or is handled improperly, Leica
will not accept any liability.
Ϙ The electric installation in the building
must conform to the national standard, e.g.
current-operated ground leakage protection (fault-current protection) is suggested.
Leica DM 500, DM500 B Manual 11
Care instructions
General instructions
Ϙ Protect the Leica DM500 or DM500 B micro-
scope against damp, vapors, acids, alkalis,
and corrosive substances. Do not store
chemicals in the vicinity.
Ϙ Protect the Leica DM500 or DM500 B micro-
scope from oil and grease. Never grease or
oil mechanical parts or sliding surfaces.
Ϙ Follow the instructions of the disinfectant
manufacturer.
Ϙ It is advisable to enter a service agreement
with Leica Service.
Cleaning coated parts and plastic parts
Ϙ Dust and dirt particles should be removed
with a soft brush or lint-free cotton cloth.
Ϙ Remove coarse debris with a moistened
disposable cloth.
Ϙ Clinging dirt can be removed using petro-
leum ether or alcohol
Ϙ Acetone, xylene or nitro-containing thin-
ners must NOT be used.
Ϙ Never use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces,
and specimens could be contaminated by
abraded particles.
Cleaning glass surfaces
Ϙ Remove dust using a fine, dry and grease-
free brush made from hair, by blowing with
a squeeze blower, or by using a vacuum.
Ϙ Remove dirt using a clean cloth that has
been moistened with distilled water.
Ϙ Remove tough dirt using alcohol, chloro-
form or petroleum ether.
Leica DM 500, DM500 B Manual 12
Accessories, maintenance and repair
Accessories
Only the following accessories may be used
with the Leica DM500 or DM500 B microscope:
Ϙ The Leica accessories described in this user
manual.
Ϙ Other accessories, provided that these have
been expressly approved by Leica as being
technically safe in this context.
Maintenance
Ϙ The Leica DM500 and DM500 B micro-
scopes are basically maintenance-free. To
ensure that they always operate safely and
reliably, we recommend that you take the
precaution of contacting the responsible
service organization.
You can arrange for periodic inspections
or, if appropriate, conclude a mainte-
nance contract with them.
Ϙ It is advisable to enter a service agreement
with Leica Service.
Ϙ For maintenance and repair, only OEM spare
parts may be used.
Repairs and service work
Ϙ Only original Leica Microsystems spare
parts may be used.
Ϙ Before opening the instruments, switch off
the power and unplug the power cable.
Ϙ Avoid contact with powered electrical
circuits, which can lead to injury.
Service address
In case of problems, please contact us as
follows:
Leica Microsystems (Switzerland) Ltd.
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)
Phone +41 71 726 33 28
Leica DM 500, DM500 B Manual 13
Electrical data and ambient conditions
Fuse Replacement
Unplug the instrument before changing any fuses. The
Leica DM500 has two fuses, which are located behind the
power cord receptacle.
Use only fuse type: 5×20 mm, 1 Amp/250V, Quick acting
•
(# 13RFAG30003)
Electrical data
DM500 Input: 100-240 VAC, 50/60 Hz, 5 W (3 W LED)
DM500 B Input: 100-240 VAC, 50/60 Hz, 0.5-0.2 A OR
4× 1.2 V AA rechargeable batteries
Use only 1.2 Volt NiMH type AA rechargeable batteries
•
with a maximum of 2700 mAh. Do not use non-recharge-
able AA batteries.
Environment
Temperature for use +10 °C … +40 °C
Storage temperature -20 °C … +52 °C
+50 °F … +104 °F
Manipulation shock 25 mm on 50 mm hard wood
Transport shock (unpacked) 100 g / 6 ms
Transport shock (packed) 800 mm free fall
Transport vibrations (unpacked) 5–200 Hz / 1.5 g
Atmospheric pressure "in use" and for storage: 500–1060 mBar
Humidity “in use” and for storage: 20–90%
Installation Category II (Overvoltage Category)
Pollution degree 2
Leica DM 500, DM500 B Manual 14
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