Leica DM2500 P, DM750 P, DM4500 P User Manual

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Leica DM4500 P, DM2500 P, DM750 P
Breaking New Ground in Polarizing Microscopy
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Brilliance
Reliability
Flexibility
Documentation
Simply Precise
Polarizing microscopes for geosciences and industry
The new Leica microscope series
is designed for all polarizing examinations: petrography, mineral­ogy, structure characterization and examination of liquid crystals. Leica’s new polarizing microscopes are ideal for a wide range of applications.
With versatile instrument options, Leica polarizing microscopes are also an ideal match for industrial analysis and quality control, such as analyzing glass, plastics, textiles and fibers or testing dis­plays in the semiconductor industry. Leica microscopes always provide the most accurate and reliable results.
Specifically designed for your application:
Leica DM4500 P for research and development
Leica DM2500 P for routine polarization applications
Leica DM750 P for university and other instructional use
Accurate results:
The new Leica polarizing microscopes will show you how easy and reliable microscopy can be. Leica’s convenient operating concept allows you to improve your workflow and concentrate entirely on the task at hand.
Advantages that speak for themselves:
Improved polarization contrast to obtain more information
from a sample
Easy operation for accurate sample evaluation in both
orthoscopy and conoscopy
Ergonomic design for user comfort
Camera and software modules can be integrated for fast, easy,
and reproducible documentation
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Leica Design by Christophe Apothéloz und Werner Hölbl
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Leica DM4500 P
The Microscope that Guides You
Automation that anticipates your next
work step:
– Automatic diaphragm setting and
light intensity
– Constant Color Intensity Control
for constant color temperature
– Condenser cap swings in and out
automatically
The right diaphragm – automatically
The Leica DM4500 P automatically detects which contrast method and objective are being used. This provides valuable consistency and reproducibility for your research. Manual diaphragm setting is no longer required, either in the transmitted light or incident light method. You can concentrate on your work – the Leica DM4500 P takes care of the rest for you.
Always in the right light
Light intensity automatically adjusts to the objective. Image bright­ness remains constant when switching objectives, which elimi­nates glare. You can always adjust the light intensity manually as well.
Constant color temperature
The Leica DM4500 P’s transmitted light axis is ideally suited for mineralogical stone identification. Its Constant Color Intensity Control automatically maintains a constant color temperature, and you no longer need neutral density filters to compensate for changes in light intensity.
Designed for use in research and development: the new Leica DM4500 P – polarizing microscopy has never been easier.
For the most precision: the Leica DM4500 P’s rotating stage
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The correct condenser setting – immediately
All condensers are designed with condenser heads that are perfectly matched optically and automatically swing in and out depending on the objective magnification. They are effective from
1.25x–100x magnification.
Oily strikes of a cholesteric liquid crystal mixture. Crossed polarizers, magnification 10x.
Images courtesy of Dr. Toralf Scharf, Institute of Microtechnology (IMT), University of Neuchâtel, S
Defective texture in planar a Crossed polarizers, magnific
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All settings at a glance
You can see all microscope settings at a glance on the easy-to­read, integrated display: information such as contrast method, orthoscopic or conoscopic mode, objective, diaphragm setting, and light intensity are clearly indicated. With this feedback, results can easily be reproduced.
Conveniently arranged functions:
– New, convenient display – Variable, programmable function buttons
Great optical quality for crystal-clear results:
– Improved conoscopy module – Precise orthoscopy
Easily assign function buttons
You can assign the function buttons to any function you want – no programming skills are required. Six conveniently located buttons behind the focus knobs provide fast and easy access to the func­tions you use most.
Perfect interaction of all functions
The interaction between the display and coding of the individual modules allows the microscope to guide your work. With just one look at the display, all relevant information is at your fingertips. For example, the display indicates when to swing the conoscopy module into or out of the beam path. You have the ability to adjust the light and diaphragm values to obtain the best conoscopic image at any time.
State-of-the art functions:
– Microscope guides you to the next work step – Displays current operation status
Everything seen on the display of the Leica DM4500 P is saved automatically. This allows you to reproduce the settings at any time.
Liquid crystal, defective texture in a hybrid aligned cell. Crossed polarizers, magnification 5x.
The Leica DM4500 P anticipates your next work step. Settings on the conoscopy module appear immediately on the display. This shows the current operating status of your instrument at all times.
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Leica DM2500 P
The Microscope that Adapts to Each User
Ergonomic design adjusts to you:
– Height-adjustable focus knobs
Convenient features let you work faster:
– Color-coded objectives and condenser
diaphragms match lenses
Safety feature protects the sample and objective:
– Integrated focus stop prevents
objective/sample collisions
Comfortable and relaxed work
No two people are alike. The Leica DM2500 P ensures that every user can work at the microscope in a relaxed manner. The height of the microscope’s focus knobs can be individually adjusted to fit each user’s exact hand size, which prevents hand, arm, and shoul­der tension and ensures a comfortable and fatigue-free posture.
Efficient and reproducible microscopy
Color-coded lenses match the color-coded field and aperture diaphragm adjustment (CDA), to ensure that the illumination con­ditions are always matched to the objective. Using a manual microscope has never been easier. With CDA, the Leica DM2500 P offers a level of reproducibility that is one-of-a-kind in its class.
Reliably and accurately adjusts to your sample
The built-in focus stop protects your samples and the front lens of the objective. For samples of equal height, the focus stop makes the focusing plane easier to reconstruct so you can concentrate entirely on your application.
The Leica DM2500 P will show you how easy and reliable polarizing microscopy can be.
Ergonomically designed to the last detail: you can adjust focus knob height to match your hand size.
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Textile fibers, crossed polarizers with lambda plate, magnification 100x.
Images courtesy of Michael Doppler, Leica Microsystems
Light augite with biotite repla rims. Black magnetite grains magnification 200x.
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Versatile and adaptable
You have a choice of two conoscopy modules to supplement the Leica DM2500 P. The advanced conoscopy module with a center­able, focusable Bertrand lens and extended field of view has been designed for advanced requirements in conoscopy. As an eco­nomical alternative, Leica offers the standard conoscopy module with a pre-focused, centerable Bertrand lens, built-in analyzer, and integrated pinhole for examining small grains.
The 4-position polarization incident light axis is ideally suited to research applications. Reflected light contrast methods such as brightfield according to Smith, quantitative polarization (POL) or fluorescence (Fluo) – provide ideal imaging conditions for miner­alogical or geological examinations. A centerable Bertrand lens module is also available for conoscopy.
Flexibility to meet your needs:
– Choice of Bertrand lens modules – Orthoscopy – 4-position Pol incident light axis – 5-position, centerable objective turret
The 5-position objective nosepiece provides individual centration for each objective, and two rotating stages are available. A 45° stage rotation with click stop is optional.
Biotite hornblende granite myrmekite (quartz-feldspar) with lambda plate. Crossed polarizers, magnification 200x.
A first on the world market: correct diaphragm setting at all times – the Color-coded Diaphragm Assistant helps set the diaphragm values needed.
Developed for everyday use on the Leica DM2500 P – the new POL rotating stage with 45° click stop to indicate the illumination positions.
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Leica DM750 P
The Microscope for Teaching
Advanced performance in a teaching
polarizing microscope:
– Standard and advanced conoscopy modules – Polarizer with notch markings – 4-position objective turret, centerable – Sturdy, compact design
Convenience that makes work easy:
– Easy-to-access control functions – Ergonomic viewing angle – Accurate angular measurement with
verniers on the rotating stage
Accurate and versatile for teaching
The Leica DM750 P is the ideal polarizing microscope for univer­sity and other instructional use, offering a standard and an advanced Bertrand lens module for unsurpassed ease of opera­tion. With a wide range of accessories and Leica’s renowned optics, the Leica DM750 P is exceptional not only for its compact, durable design, but also for its efficiency and ease of operation.
Designed for optical brilliance and long life illumination
The standard Köhler field diaphragm and magnetically fixed blue filter provide vivid, pin-sharp images. The 2,000-hour, 35-watt halogen lamp saves hundreds of dollars in replacement bulb cost over the life of the microscope. Based on the same optical plat­form as Leica Microsystems’ research microscope line, students enjoy outstanding optical performance and full access to virtually all accessories from the Leica Microsystems microscope product line.
Developed for college teaching and research use: the Leica DM750 P.
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Maximum ease of use and high optical brilliance are the outstanding features of the Leica DM750 P.
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Camera and Software Modules Complete the System
Ready to expand at any time
To seamlessly interface with the new Leica polarizing micro­scopes, Leica offers a comprehensive camera and software solu­tion for fast, convenient documentation of your work. You can expand your system at any time using Leica’s cameras and appli­cation-specific software modules. All future software and hard­ware components from Leica will operate on a uniform interface.
Archiving and documentation is easy
The basic core functionality of the Leica Application Suite (LAS) is provided with every Leica microscope and digital camera as part of an integrated system solution. Together, the combined system provides an intelligent, automated microimaging environment. LAS is the basic software for microscope configuration and con­trol, and provides a platform for acquiring, analyzing, and pro­cessing the highest quality digital images.
LAS Reticule for comparison and measurement
The LAS Reticule application provides electronic tools for displaying live images and overlaying different types of measuring reticules. LAS Reticule provides visual feedback about the approximate size of the field of view. In this way, object size comparisons and distribution measure­ments can be carried out quickly and effortlessly.
Leica’s complete polarizing microscope
systems integrate the following components:
– Leica polarizing microscope – Leica Digital FireWire Camera (DFC) – Leica Application Suite (LAS) software
Advanced interactive measurement
The Interactive Measurement module of the Leica Application Suite has been designed for particularly difficult mea­surements. Using this module, sam­ples can be individually counted and assigned to an identified class as well.
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Modular, Customized Configurations –
Microscopes Designed for You
Flexibility that gives the freedom you need:
– Wide selection of POL objectives
Compatibility that knows no bounds:
– Fully compatible components across Leica’s
polarizing microscope product line
– Wide selection of analyzers, polarizers,
and compensators – Full wave & quarter wave plates are available – Wide selection of POL observation tubes
The result of combining maximum precision and optimum ergonomic design – the 360° analyzer.
Flexibility – Designed for you
Flexible to the last detail. All Leica polarizing microscope compo­nents can be configured for all microscopes in the polarizing line. For example, you can choose from over twenty POL objectives for the Leica DM4500 P, DM2500 P or DM750 P. The optical possibili­ties are unlimited. You will enjoy the benefits provided by this complete system when using the new 360° analyzer, the 360° polarizer or even with full wave plates. All components can be used for classroom teaching, everyday routine work, and research.
Leica’s entire line of DIN standard compensators can be used in all Leica polarizing microscopes, as can the attachable mechanical stage for accurate sample positioning. This always ensures flexible interchange and replacement of parts.
Flexibility is key. All of Leica’s rotating stage polarizing microscopes feature attachable, interchangeable mechanical stages.
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Technical Data
Objective turret
Objectives
Usable field
of view
Contrast method
Changeover Color reproduction
Transmitted light
Incident light
Leica DM750 P
4x (M25), centerable
HI Plan POL N Plan POL
Immersion objectives
20 mm
Manual
Polarization contrast Orthoscopy Conoscopy Brightfield Phase contrast
Darkfield Polarization contrast Brightfield
Leica DM2500 P
5x (M25), centerable
HI Plan POL N Plan POL PL Fluotar POL Immersion objectives
25 mm
Manual
Polarization contrast Orthoscopy Conoscopy Brightfield Phase contrast DIC Darkfield Polarization contrast Brightfield Darkfield* DIC Fluorescence
Leica DM4500 P
6x (M25), centerable, absolute encoded
HI Plan POL N Plan POL PL Fluotar POL Immersion objectives
25 mm
Motorized CCIC: Constant Color Intensity Control
Polarization contrast Orthoscopy Conoscopy Brightfield Phase contrast DIC Darkfield Polarization contrast Brightfield Darkfield* DIC Fluorescence
Conoscopy
Transmitted
light axis Illumination Operation
Incident light axis
Condensers
Focus drive
* on request
Bertrand lens cube in new IL axis Bertrand lens module (AB module) Advanced conoscopy module
12 V 35 W halogen lamp Manual User guidance with CDA
Manual User guidance with CDA
Manual changeover User guidance with CDA
Manual, 2-gear gearbox
Bertrand lens cube
Bertrand lens module (AB module) Advanced conoscopy module
12 V 100 W halogen lamp Manual User guidance with CDA
Manual User guidance with CDA
Manual changeover User guidance with CDA
Manual, height-adjustable, Focus stop, 2 or 3-gear gearbox
Fully integrated conoscopy beam path User guidance with display feedback
12 V 100 W halogen lamp Motorized Integrated illumination manager
Motorized Integrated illumination manager, round and rectangular field diaphragms for ocular or camera observation
Motorized changeover of condenser head, 7x condenser disc, polarizer
Manual, 2-gear gearbox
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“With the user, for the user” Leica Microsystems
Leica Microsystems operates globally in four divisions, where we rank with the market leaders.
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The Leica Microsystems Life Science Division supports the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement, and analysis of microstructures. Our strong focus on understanding scientific applications puts Leica Microsystems’ customers at the leading edge of science.
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The Leica Microsystems Industry Division’s focus is to support customers’ pursuit of the highest quality end result. Leica Microsystems provide the best and most innovative imaging systems to see, measure, and analyze the micro­structures in routine and research industrial applications, materials science, quality control, forensic science inves­tigation, and educational applications.
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The Leica Microsystems Biosystems Division brings his­topathology labs and researchers the highest-quality, most comprehensive product range. From patient to pa­thologist, the range includes the ideal product for each histology step and high-productivity workflow solutions for the entire lab. With complete histology systems fea­turing innovative automation and Novocastra™ reagents, Leica Microsystems creates better patient care through rapid turnaround, diagnostic confidence, and close cus­tomer collaboration.
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The statement by Ernst Leitz in 1907, “with the user, for the user,” describes the fruitful collaboration with end users and driving force of innovation at Leica Microsystems. We have developed five brand values to live up to this tradition: Pioneering, High-end Quality, Team Spirit, Dedication to Science, and Continuous Improvement. For us, living up to these values means: Living up to Life.
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