Hanna Instruments HI 253 User Manual

Instruction Manual

Functions

pH Calibration - 3 points

RCL

LOG

CLR

SET

RANGE

GLP

CFM

CAL

pH / ORP / Ion Meter

HI 253

Confirm Calibration

in selected pH Buffer

CFM

Select pH Buffer

Start/Exit Calibration

CAL

Select pH/ORP/ mV/Ion

Scroll Log Data

Rel

RANGE

GLP

Show GLPData

LOG

StoreLog Data

SET/CLR

Set Instrument Parameters

Clear Calibration/Log Data

RCL

RecallLog Data

HI 253

Microprocessor-Based

pH/mV/Ion/ºC

Bench Meter

www.hannainst.com

Dear Customer, Thank you for choosing a Hanna Instruments product. Please read this instruction manual carefully before using the instrument. This manual will provide you with the necessary information for correct use of

the instrument, as well as a precise idea of its versatility. If you need additional technical information, do not hesitate to e-mail us at

tech@hannainst.com or turn to the back cover for our worldwide contact list. This instrument is in compliance with directives.

WARRANTY

HI 253 is guaranteed for two years against defects in workmanship and materials when used for its intended purpose and maintained according to instructions. Electrodes and probes are guaranteed for six months. This warranty is limited to repair or replacement free of charge.

Damage due to accidents, misuse, tampering or lack of prescribed maintenance is not covered.

If service is required, contact the dealer from whom you purchased the instrument. If under warranty, report the model number, date of purchase, serial number and the nature of the problem. If the repair is not covered by the warranty, you will be notified of the charges incurred. If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization number from the Technical Service department and then send it with shipping costs prepaid. When shipping any instrument, make sure it is properly packed for complete protection.

TABLE OF CONTENTS

WARRANTY ......................................................................................................................... 2

PRELIMINARY EXAMINATION .............................................................................................. 3

GENERAL DESCRIPTION....................................................................................................... 3

FUNCTIONAL DESCRIPTION ................................................................................................ 4

HI 253 SPECIFICATIONS .................................................................................................... 5

OPERATIONAL GUIDE ......................................................................................................... 7

pH CALIBRATION ................................................................................................................ 10

ION CALIBRATION ............................................................................................................... 13

GOOD LABORATORY PRACTICE (GLP)....................................................................................15

LOGGING FUNCTION..............................................................................................................18

SETUP................................................................................................................................... 22

TEMPERATURE CALIBRATION (for technical personnel only)............................................ 26

mV CALIBRATION (for technical personnel only)............................................................... 27

RELATIVE mV CALIBRATION............................................................................................... 28

PC INTERFACE .................................................................................................................... 29

pH VALUES AT DIFFERENT TEMPERATURES .................................................................... 31

ELECTRODE CONDITIONING & MAINTENANCE .................................................................. 32

TROUBLESHOOTING GUIDE ............................................................................................... 35

TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS ............................................... 36

ACCESSORIES ...................................................................................................................... 37

PRELIMINARY EXAMINATION

Remove the instrument from the packing material and examine it carefully to make sure that no damage has occurred during shipping. If there is any damage, notify your Dealer or the nearest Hanna Customer Service Center.

Each instrument is supplied with:

• HI 1131B Glass-body Combination pH Electrode with 1 m (3.3') Cable

• HI 7669/2W Temperature Probe

• HI 76404 Electrode Holder

• pH 4.01 & 7.01 Buffer Solutions (20 mL each)

• HI 7071S Electrolyte Solution

• 12VDC Power Adapter

• Instruction Manual

Note: Save all packing material until you are sure that the instrument

functions correctly. All defective items must be returned in the original packing with the supplied accessories.

GENERAL DESCRIPTION

The HANNA HI 253 is a logging microprocessor-based pH, mV, Ion and temperature bench meter. Relative mV feature is also provided.

pH measurements are compensated for temperature effect manually or automatically with the HI 7669/2W temperature probe.

One, two or three-point calibration is possible, using five memorized buffers (4.01, 6.86, 7.01, 9.18 and 10.01 pH).

The GLP feature provides a guarantee of data consistency. An alarm time-out is available to alert the user that too much time elapsed

since the last pH calibration. The instrument can store data in memory at the user’s request for later

retrieval.

Hanna Instruments reserves the right to modify the design, construction and appearance of its products without advance notice.

FUNCTIONAL DESCRIPTION

Front Panel

Primary LCD

Secondary LCD

POWER 12VDC

INPUT

REF

TEMP

12 13 14

Rear Panel

CAL

CFM READY

1 2 3

BUF

WRONG

LOG mVpH

TIME% DATE INTV

BUFFER pH

RS232

Functions pH Calibration - 3 points

RCL

LOG

CLR

SET

RANGE

GLP

CFM

CAL

CFM

CAL

RANGE

GLP

LOG

SET/CLR

RCL

pH / ORP / Ion Meter

HI 253

Confirm Calibration in selected pH Buffer

Select pH Buffer

Start/Exit Calibration

Select pH/ORP/ mV/Ion Scroll Log Data

Rel

Show GLPData

StoreLog Data

Set Instrument Parameters Clear Calibration/Log Data

Recall Log Data

ppm

1) Liquid Crystal Display (LCD).

2) CFM/GLP key, to confirm different values or to display Good Laboratory Practice information.

3) CAL key, to enter and exit/escape calibration mode.

4) /ºC key, to manually decrease temperature value or other parameters.

5) /ºC key, to manually increase temperature value or other parameters.

6) RCL key, to enter/exit recall mode.

7) LOG key, to store measured data.

8) SET/CLR key, to enter/exit setup mode, clear pH calibration or delete logged data.

9) RANGE key, to select measurement range or to switch focused data.

10) ON/OFF switch.

11) Power supply socket.

12) BNC electrode connector.

13) Electrode reference socket.

14) Temperature probe socket.

15) RS232 serial communication connector.

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HI 253

SPECIFICATIONS (cont.)

OPERATIONAL GUIDE

CAL

CFM READY

1 2 3

BUF

WRONG

LOG mV pH

TIME% DATE INTV

BUFFER pH

ppm

POWER CONNECTION

Plug the 12 VDC adapter into the power supply socket.

Note: This instrument uses non volatile memory to retain the pH, mV,

temperature calibrations and all other settings, even when unplugged.

Note: Make sure a fuse protects the main line.

ELECTRODE AND PROBE CONNECTIONS

For pH or ORP measurements connect an electrode with internal reference to

the BNC connector on the back of the instrument.

For electrodes with a separate reference connect the electrode’s BNC to the BNC

connector and the reference electrode plug to the reference socket.

For temperature measurements and automatic temperature compensation

connect the temperature probe to the appropriate socket.

INSTRUMENT START-UP

• Turn the instrument on by pressing the ON/OFF switch.

• All LCD tags are displayed and a beep is sounded while the instrument performs a self test.

pH MEASUREMENTS

Make sure the instrument has been calibrated before taking pH measurements.

• Submerge the electrode tip and the temperature probe approximately 4 cm (1½”) into the sample to be tested and stir gently. Allow time for the electrode to stabilize.

• The pH is displayed on the primary LCD and the temperature on the secondary LCD.

• If the reading is out of range, the closest full-scale value will be displayed blinking on the primary LCD.

If measurements are taken successively in different samples, it is recommended

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to rinse the electrode thoroughly with deionized water or tap water and then with some of the next sample to prevent cross-contamination.

The pH reading is affected by temperature. In order to measure the pH accurately, the temperature effect must be compensated for. To use the Automatic Temperature Compensation feature, connect and submerge the

HI 7669/2W temperature probe into the sample as close as possible to the electrode and wait for a few seconds.

If the temperature of the sample is known, simple manual compensation can be performed by disconnecting the temperature probe.

The display will then show the default temperature of 25 ºC or the last temperature reading with the“ºC” tag blinking.

The temperature can now be adjusted with the ARROW keys (from –9.9 ºC to 120.0 ºC).

mV/ORP MEASUREMENTS

An optional ORP electrode must be used to perform ORP measurements (see Accessories).

Oxidation-Reduction Potential (REDOX) measurements provide the quantification of the oxidizing or reducing power of the tested sample.

To correctly perform a REDOX measurement, the surface of the ORP electrode must be clean and smooth.

• Press RANGE to enter mV range.

• Submerge the tip of the ORP electrode 4 cm (1½”) into the sample to be tested and allow a few seconds for the reading to stabilize.

• The instrument displays the mV reading on the primary LCD and the temperature on the secondary LCD.

• If the reading is out of range, the closest full-scale value will be displayed blinking on the primary LCD.

RELATIVE mV MEASUREMENTS

ppm

• Press RANGE until “rEL” message will be displayed on the secondary LCD line for about one second and “mV” tag will blink. After one second the temperature will be displayed on the secondary LCD.

The reading displayed by the instrument is equal to the difference between the current mV input value and relative mV offset established in the relative mV calibration.

ION MEASUREMENTS

• Press RANGE to enter ppm measurement mode.

• Submerge the tip of the ISE electrode 4 cm (1½”) into the sample to be tested and allow a few seconds for the reading to stabilize.

• The instrument displays the ppm reading on the primary LCD and the temperature on the secondary LCD line.

TEMPERATURE MEASUREMENTS

Connect the HI 7669/2W temperature probe to the appropriate socket and turn the instrument on.

Immerse the temperature probe into the sample and allow the reading on the secondary LCD to stabilize.

pH CALIBRATION

BUF

CAL

BUFFER pH

BUF

Calibrate the instrument frequently, especially if high accuracy is required. The instrument should be recalibrated :

• Whenever the pH electrode is replaced.

• At least once a week.

• After testing aggressive chemicals.

• If “CAL” “INTV” tags are blinking during measurement.

Every time you calibrate the instrument use fresh buffers and perform an electrode Cleaning Procedure (see page 34).

PREPARATION

Pour small quantities of the buffer solutions into clean beakers. If possible, use plastic or glass beakers to minimize any EMC interferences.

For accurate calibration and to minimize cross-contamination, use two beakers for each buffer solution. One for rinsing the electrode and one for calibration.

If you are measuring in the acidic range, use pH 7.01 or 6.86 as first buffer and pH 4.01 as second buffer. If you are measuring in the alkaline range, use pH

7.01 or 6.86 as first buffer and pH 10.01 or 9.18 as second buffer.

PROCEDURE

Calibration has a choice of 5 memorized buffers: pH 4.01, 6.86, 7.01, 9.18 and 10.01.

It is recommended to perform a two or three-point calibration. However, the instrument also allows one-point calibration, as described on page 12.

THREE-POINT CALIBRATION

• Immerse the pH electrode and the temperature probe approximately 4 cm (1½”) into a buffer solution of your choice (pH 4.01, 6.86, 7.01, 9.18 or 10.01) and stir gently. The temperature probe should be close to the pH electrode.

• Press CAL. The “CAL” and “

” tags will appear and the “7.01” buffer will be displayed on the secondary LCD.

• If necessary, press the ARROW keys to select a different buffer value.

• The “ ” tag will blink on the LCD until the reading is

CAL

BUF

BUFFER pH

stable.

• When the reading is stable and close to the selected buffer, the “READY” tag will be displayed and the “CFM” tag will blink.

• Press CFM to confirm calibration.

• The calibrated value will be displayed on the primary LCD and the second expected buffer value on the secondary LCD.

Note: The instrument will automatically skip the buffer used for the first point.

It also skips 6.86 if 7.01 buffer was used and viceversa. Likewise, it will skip 9.18 if 10.01 buffer was used and viceversa.

• After the first calibration point is confirmed, immerse the pH electrode and the temperature probe approximately 4 cm (1½”) into the second buffer solution and stir gently. The temperature probe should be close to the pH electrode.

• If necessary, press the ARROW keys to select a different buffer value.

• The “ ” tag will blink on the LCD until the reading is stable.

• When the reading is stable and close to the selected buffer, the “READY” tag will be displayed and the “CFM” tag will blink.

• Press CFM to confirm calibration.

• The calibrated value will be displayed on the primary LCD and the third expected buffer value on the secondary LCD.

Note: The instrument will automatically skip the buffers used for the first and

second calibration points.

• After the second calibration point is confirmed, immerse the pH electrode and the temperature probe approximately 4 cm (1½”) into the third buffer solution and stir gently. The temperature probe should be close to the pH electrode.

• If necessary, press the ARROW keys to select a different buffer value.

• The “ ” tag will blink on the LCD until the reading is stable.

• When the reading is stable and close to the selected buffer, the “READY” tag will be displayed and the “CFM” tag will blink.

• Press CFM to confirm calibration.

• The instrument stores the calibration values and returns to normal measurement mode.

Note: • If the value measured by the instrument is not close to the

BUF

selected buffer, “WRONG” “

” and “WRONG” “ ” tags will blink alternately. In this case, check if the correct buffer has been used or regenerate the electrode by following the Cleaning Procedure (see page 34). If necessary, change the buffer or the electrode.

• If “WRONG”, “Buffer pH” tags and “Old” tag on the secondary LCD are displayed blinking, an inconsistency between new and previous (old) calibration is detected. Clear calibration parameters by pressing SET/CLR and proceed with calibration from the current calibration point (the instrument will keep all confirmed values during current calibration).

• The “WRONG” tag and temperature value are displayed blinking if temperature reading is out of the defined temperature range of the buffer. Calibration cannot be confirmed in this situation.

• Press RANGE to display the temperature reading on the LCD during calibration.

TWO-POINT CALIBRATION

• Proceed as described in “Three-point calibration” section.

• Press CAL after the second calibration point was confirmed.

The instrument will return to measurement mode and will memorize the two-point calibration data.

ONE-POINT CALIBRATION

• Proceed as described in “Three-point calibration” section.

• Press CAL after the first calibration point was confirmed.

The instrument will return to measurement mode and will memorize the one-point calibration data.

Note: Calibration parameters are evaluated taking in consideration the new

values for the confirmed buffers in current calibration and the old values, if existing, for the other buffers. To clear old calibration values, press SET/CLR before exiting calibration.

ION CALIBRATION

BUF

CAL

BUF

ppm

CAL

BUF

ppm

For greatest accuracy, it is recommended to calibrate the instrument frequently. Due to electrode conditioning time, the electrode must be kept immersed a few

seconds to stabilize. The user will be guided step by step during calibration with easy to follow tags on the display. This will make the calibration a simple and error-free procedure.

PREPARATION

In SETUP menu, select the proper ion charge. Note: If “undF” option is selected in SETUP menu, calibration must be

performed in two points, otherwise “----” message will be displayed on the LCD if exiting calibration after confirming the first used buffer.

Pour small quantities of the buffer solutions into clean beakers. If possible, use plastic beakers to minimize any EMC interferences.

For accurate calibration and to minimize cross-contamination, use two beakers for each buffer solution. One for rinsing the electrode and one for calibration.

PROCEDURE

One or two-point calibration is available, using five memorized buffers: 0.1, 1, 10, 100, 1000 ppm.

Select Ion measurement range by pressing RANGE. Remove the protective cap from the ISE electrode.

TWO-POINT CALIBRATION

• Immerse the ISE electrode approximately 4 cm (1½”) into the selected solution and stir gently.

• Press CAL. The primary LCD will display the ppm value using the current offset and slope. The “CAL” and “

” tags will appear and 0.1 ppm buffer will

be displayed on the secondary LCD.

• If necessary, press the ARROW keys to select a different buffer value.

• The “ ” tag will blink on the LCD until the reading is stable.

• When the reading is stable and close to the selected buffer, the “READY” tag will be displayed and the “CFM” tag will blink.

• Press CFM to confirm calibration.

• The calibrated value will be displayed on the primary LCD and the second expected buffer value on the secondary LCD.

Note: The instrument will automatically skip the buffer used for the first point.

BUF

• After the first calibration point is confirmed, immerse the ISE electrode approximately 4 cm (1½”) into the second calibration solution.

• If necessary, press the ARROW keys to select a different buffer value.

• The “ ” tag will blink on the LCD until the reading is stable.

• When the reading is stable and close to the selected buffer, the “READY” tag will be displayed and the “CFM” tag will blink.

• Press CFM to confirm calibration.

• The instrument stores the calibration value and returns to normal measurement mode.

Note: • If the mV value is out of the mV range (±2000), “WRONG” “

and “WRONG” “ ” tags will blink alternately. In this case, check if the correct buffer has been used or refresh the electrode by following the Cleaning Procedure (see page 34). If necessary, change the buffer or the electrode.

• If the new slope is out of the slope window, “WRONG” “

” and

“WRONG” “ ” tags will blink alternately. In this case, check if the correct buffer has been used or refresh the electrode by following the Cleaning Procedure. If necessary, change the buffer or the electrode.

Slope window is between ±20 mV and ±105 mV if ion charge is not specified (undF option in SETUP menu) or between 50% and 120% of default slope for the corresponding ion charge.

Default slope value (mV/decade):

–59.16 (monovalent anion) - ion charge is –1

59.16 (monovalent cation) - ion charge is 1

–29.58 (divalent anion) - ion charge is –2

29.58 (divalent cation) - ion charge is 2 100 - ion charge is “undF”

• Press SET/CLR during calibration if you want to clear calibration values and set to default. The instrument will display “CLR” message and will return to measurement mode.

• Press RANGE to display the temperature reading on the LCD during calibration.

ONE-POINT CALIBRATION

• Press CAL after first calibration point was confirmed. The instrument memorizes the one-point calibration parameters and returns to measurement mode.

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