Hanna Instruments HI 222 User Manual

Instruction Manual

HI 222

pH/mV/ºC

Bench Meter

with Calibration Check

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Manufacturer since 1978

www.hannainst.com

1

Dear Customer,
Thank you for choosing a Hanna Instruments product.
Please read this instruction manual carefully before using the instrument.
This manual will provide you with the necessary information for correct use of

the instrument, as well as a precise idea of its versatility.
For additional technical information, do not hesitate to e-mail us at

[e-mail] or refer to back cover for our worldwide contact list.
This instrument is in compliance with the directives.

WARRANTY

HI 222 is guaranteed for two years against defects in workmanship and
materials when used for their intended purpose and maintained according to
instructions. Electrodes and probes are guaranteed for a period of six months.
This warranty is limited to repair or replacement free of charge.

Damage due to accidents, misuse, tampering or lack of prescribed mainte­nance are not covered.

If service is required, contact the dealer from whom you purchased the
instrument. If under warranty, report the model number, date of purchase,
serial number and the nature of the problem. If the repair is not covered by
the warranty, you will be notified of the charges incurred. If the instrument is
to be returned to Hanna Instruments, first obtain a Returned Goods Authori­zation number from the Technical Service department and then send it with
shipping costs prepaid. When shipping any instrument, make sure it is
properly packaged for complete protection.

TABLE OF CONTENTS

PRELIMINARY EXAMINATION ........................................................................................... 3

GENERAL DESCRIPTION.................................................................................................... 3

FUNCTIONAL DESCRIPTION ............................................................................................. 4

HI 222 SPECIFICATIONS .................................................................................................. 5

OPERATIONAL GUIDE ....................................................................................................... 6

pH CALIBRATION ................................................................................................................ 8

ENHANCED CALIBRATION MESSAGES ....................................................................11

ELECTRODE CONDITION AND RESPONSE TIME ............................................................13

GOOD LABORATORY PRACTICE (GLP) .........................................................................14

LOGGING .........................................................................................................................17

SETUP ............................................................................................................................ 21

TEMPERATURE CALIBRATION (for technical personnel only) .............................. 25

mV CALIBRATION (for technical personnel only) .................................................. 27

PC INTERFACE ............................................................................................................... 28

pH VALUES AT DIFFERENT TEMPERATURES .................................................................... 30

ELECTRODE CONDITIONING & MAINTENANCE .............................................................. 31

TROUBLESHOOTING GUIDE .......................................................................................... 33

TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS ............................................35

ACCESSORIES ................................................................................................................ 36

2

PRELIMINARY EXAMINATION

Remove the instrument from the packing material and examine it carefully to
make sure that no damage has occurred during shipping. If there is any
damage, notify your Dealer or the nearest Hanna Customer Service Center.

Each instrument is supplied complete with:

• HI 1048P Glass-body pH Electrode with 1 m (3.3 “) cable

• HI 7669/2W Temperature Probe

• HI 76404 Electrode Holder

• HI 50003 & HI 70007 pH Calibration Solutions (20 mL each)

• HI 700635 Cleaning Solution for wine deposits (2x20 mL)

• HI 700636 Cleaning Solution for wine stains (2x20 mL)

• HI 7082 Electrode Refilling Solution (30 mL) & 5 mL Syringe

• 12 VDC Power Adapter

• Instruction Manual

Note: Save all packing material until you are sure that the instrument

functions correctly. All defective items must be returned in the original
packing with the supplied accessories.

GENERAL DESCRIPTION

HI 222 is a logging microprocessor-based pH/ORP/temperature bench meter
with Calibration Check.

Calibration Check performs a set of diagnostic tests during calibration using the
history of electrode slope and offset to detect problems that can cause loss of
accuracy.

Calibration Check Features are listed below:

• Enhanced Calibration Messages

During calibration the user is warned if one or more parameters are not suitable
to perform an accurate calibration.

• Electrode Condition on LCD Display

Determined from the electrode offset and slope.

• Electrode response time on LCD Display

Determined from electrode performance during calibration.

• Calibration Alarm Time Out

Can be programmed from 1 to 7 days or can be disabled.

Other features include 1 or 2-point pH calibration with seven memorized
buffers (1.68, 3.00, 6.86, 7.01, 9.18, 10.01 and 12.45 pH), logging up to
100 samples, last calibration date and data (GLP), pH reading with manual
or automatic temperature compensation and PC interface software.

The pH 3.00 memorized buffer makes the instrument useful for wine pH
measurements.

3

Front Panel

1

FUNCTIONAL DESCRIPTION

POOR GOOD

Electrode
Response

SLOW

FAST

Primary LCD

pH

Secondary LCD

ATC

C

Electrode
Condition

10

2

Functions pH Calibration

Select pH/ORP

3

5

7

CAL

4

6

LOG

RANGE

CFM

LOG

Store Log Information

GLP

RCL

Recall Log Information

Set Instrument Parameters

SET/CLR

Clear Log/Cal Information

CC

CFM/GLP

Show GLP Information

RCL

CAL

Start Calibration

st

/

Select 1 Buffer

st

CFM

Confirm 1 Buffer

nd

/

Select 2 Buffer

nd

CFM

Confirm 2 Buffer

HI 222 Calibration Check
Microprocessor pH Meter

RANGE

SET
CLR

8

9

Rear Panel

POWER

12VDC

11

1) Liquid Crystal Display (LCD)

2) CFM / GLP key, to confirm different values or to display Good Laboratory
Practice information

3) CAL key, to enter and exit/escape the calibration mode

4) / ºC key, to manually decrease temperature, or other parameters

5) / ºC key, to manually increase temperature, or other parameters

6) RCL key, to enter or exit the recall mode

7) LOG key, to store measured data

8) RANGE key, to select the measurement range or switch the focused data

9) SET / CLR key, to enter the Setup mode or clear calibration history

10) ON / OFF switch

11) Power supply socket

12) BNC connector for electrode

13) Pin input socket

14) Temperature probe socket

15) RS232 serial communication port

Note: Pin input socket can not be used as reference input for separate

electrode reference.

INPUT TEMP

13

12

RS232

14

15

4

HI222 SPECIFICATIONS

Range -2.00 to 16.00 pH

±699.9 mV / ±2000 mV

-20.0 to 120.0 °C

Resolution 0.01 pH

0.1 mV / 1 mV

0.1 °C

Accuracy ±0.01 pH
(@20°C/68°F) ±0.2 mV / ±1 mV

±0.5 °C

pH Calibration 1 or 2-point, automatic,

with 7 memorized pH buffer values
(1.68, 3.00, 6.86, 7.01, 9.18, 10.01, 12.45)

Temperature Compensation Automatic or manual, from -20 to 120°C
pH Electrode HI 1048P glass-body, single junction,

(included) refillable, BNC + pin
Temperature probe HI 7669/2W stainless steel probe

(included)
Input impedance 10
Computer Interface RS232, optoisolated
Logging 100 points
Environment 0 to 50°C (32 to 122°F)

Power supply 12 VDC adapter (included)
Dimensions 240x182x74 mm (9.4x7.1x2.9")
Weight 1.1 kg (2.5 lb.);

12

Ω

max. 95% RH non-condensing

kit with holder: 2.5 kg (5.5 lb.)

5

OPERATIONAL GUIDE

POWER CONNECTION

Plug the 12 VDC adapter into the power supply socket.
Note: These instruments use non volatile memory to retain the pH, mV,

temperature calibrations and all other settings, even when unplugged.

Note: Make sure a fuse protects the mains line.

ELECTRODE AND PROBE CONNECTIONS

For HANNA P Type pH or ORP electrodes (with internal reference) connect the
electrode’s BNC to the socket on the back of the instrument and the pin to the
appropriate socket.

Note: Electrode condition and response information is displayed on the bar

graph gauges. During the day the calibration is performed only if
HANNA P type (PIN) electrodes are used.

If the electrode is not recognized as a HANNA P type electrode, the bar
graph gauges will blink (25 seconds OFF, 4 seconds ON, full bar graph).

For temperature measurement and automatic temperature compensation
connect the temperature probe to the appropriate socket.

INSTRUMENT START-UP

• Turn the instrument on by pressing the ON/OFF switch.

• All LCD tags are displayed and a beep is sounded

while the instrument performs a self test.

CFM

CAL

DUE

POOR GOOD

CHECK

WRONG

CONTAMINATED

ELECTRODE

CLEAN

SLOW

BUFFER
BUFFER

Unscrew electrode refilling cap

FAST

CLEAR CAL if new electrode

mV

pH

SETUP

LOG

BUFFER 12

ATC

MTC

RCL

DEL

• The “Unscrew electrode refilling cap” message reminds the user to loosen

or remove the electrode refilling cap to improve the electrode’s response
time.

• The instrument automatically defaults to pH measurement mode unless a

HANNA P type ORP electrode is detected.

pH MEASUREMENT

Make sure the instrument has been calibrated before
taking pH measurements.

• Submerge the tip (4 cm/1½”) of a properly condi-

tioned electrode (see page 31) and the tempera­ture probe into the sample to be tested. Allow time
for the electrode to stabilize.

6

• The pH is displayed on the primary LCD and the temperature on the

secondary LCD.

ATC

POOR GOOD

SLOW

FAST

pH

• The pH reading is out of range, the closest full-scale value will be displayed

blinking on the primary LCD.

• It is also possible to view the mV reading by pressing the RANGE key.

POOR GOOD

SLOW

FAST

mV

If measurements are taken successively in different samples, it is recommended
to rinse the electrode thoroughly with deionized water or tap water and then
with some of the next sample to prevent cross-contamination.

The pH reading is affected by temperature. In order to measure the pH
accurately, this temperature effect must be compensated for. To use the
Automatic Temperature Compensation feature, connect
and submerge the HI 7669/2W temperature probe into

ATC

C

the sample as close to the electrode as possible and wait
for a few minutes.

If the temperature of the sample is known, manual compensation can be
performed by disconnecting the temperature probe.

The display will then show the default temperature of 25 °C

MTC

C

or the last recorded temperature reading with the “°C”
symbol blinking.

The temperature can now be adjusted with the ARROW keys (from -20.0 ºC
to 120.0 ºC).

ORP MEASUREMENTS

An optional ORP electrode must be used to perform ORP measurements (see
Accessories).

Oxidation-Reduction Potential (REDOX) measurements provide the quantifica­tion of the oxidizing or reducing power of the tested sample.

To correctly perform a REDOX measurement, the surface of the ORP electrode
must be clean and smooth. Pretreatment solutions are available to condition
the electrode and speed up the response time.

C

7

• The instrument automatically defaults to the ORP

measurement mode if HANNA P type ORP electrode
is detected.

• Submerge the ORP electrode tip (4 cm/1½”) into the

sample. Allow a few minutes for the reading to sta­bilize.

• The instrument displays the mV reading on the primary LCD.

• If the reading is out of range, the closest full-scale value will be displayed

blinking on the primary LCD.

TAKING TEMPERATURE MEASUREMENTS

Connect the HI 7669/2W temperature probe and turn the
instrument on. Dip the temperature probe into the sample and
allow the reading on the secondary LCD to stabilize.

pH CALIBRATION

Calibrate the instrument frequently, especially if high accuracy is required. For
best results and constant display of electrode condition and electrode response
on the bar graph gauges we suggest at least a daily calibration.

The instrument should be re-calibrated:

• Whenever the pH electrode is replaced.

• At least once a day.

• After testing aggressive chemicals.

• If high accuracy is required.

• If CAL DUE message is displayed during measurement.

Every time you calibrate the instrument use fresh buffers and perform an
electrode cleaning procedure (see page 31).

PREPARATION

Pour small quantities of the buffer solutions into clean beakers. If possible, use
plastic or glass beakers to minimize any EMC interferences.

For accurate calibration and to minimize cross-contamination, use two beakers
for each buffer solution. One for rinsing the electrode and one for calibration.

8

PROCEDURE

The user has a choice of 7 memorized buffers: pH 1.68, 3.00, 6.86, 7.01,

9.18, 10.01 and 12.45.

It is always recommended to perform a two-point calibration. The instrument
however allows a one-point calibration, as described on page 10.

TWO-POINT CALIBRATION

For most applications it is recommended that pH 7.01 or 6.86 buffers to be
used as the first calibration point and pH 3.00 (for acidic samples) or pH

9.18/10.01 (for alkaline samples) as the second calibration point.

Note: The pH 12.45 buffer is not for general measurement; use only if the

sample is very alkaline to avoid sodium error.

• Immerse the pH electrode and the temperature probe approximately
4 cm (1½”) into the buffer solution of your choice (pH 1.68, 3.00,

6.86, 7.01, 9.18, 10.01 or 12.45) and stir gently. The temperature
probe should be close to the pH electrode.

• Press the CAL key. “CAL” and “pH” tags will be on, and the “CLEAR
CAL if new electrode” tag will blink.

CAL

CLEAR CAL if new electrode

pH

• Press the CLR key if you are using a new electrode or want to clear the
calibration history. The instrument will display the “donE” message for
a few seconds.

It is very important to clear the calibration history when a new electrode is
used because all error and warning messages that appear during calibration
depend on the calibration history.

• Press the CAL key or wait a few seconds to continue.

Note: The above behavior appears only if calibration history is not empty.

• The instrument will display the measured pH on the primary LCD, and
the most common buffer (“7.01”) on the secondary LCD, along with
“CAL”, “pH” and “BUFFER 1” tags.

• The “ ” tag will blink until the reading has stabilized.

CAL

9

pH

ATC

BUFFER 1

• Press the ARROW keys to select a different buffer value, if necessary.

• When the reading is stable and close to the selected buffer, the “CFM”
tag will blink and if enabled, an audible signal will sound.

CFM

CAL

pH

ATC

BUFFER 1

• Press the CFM key to confirm calibration. The instrument will ask for a
second calibration buffer and will display the measured pH on the first LCD
and the second calibration buffer on the second LCD.

CAL

pH

ATC

BUFFER 2

• If necessary, press the ARROW keys to select a different buffer value.

Note: The instrument will automatically skip the buffer used for the first point.

It also skips 6.86 if 7.01 was used, and vice versa. Likewise, it will skip

9.18 if 10.01 has been used, and vice versa.

• Rinse the electrode in one of the beakers of the second buffer solution, then
immerse the pH electrode and the temperature probe approximately 4 cm
(1½”) into the second buffer solution and stir gently. The temperature
probe should be close to the pH electrode.

• The “ ” indication will blink on LCD until the reading has stabilized.

• When the reading is stable and close to the selected buffer, the
“CFM” tag will blink.

• Press the CFM key to confirm the calibration.

The instrument will return to normal operational mode and will memorize the
calibration data.

Note: Press the RANGE key any time during calibration to display the

temperature reading.

CAL

ATC

pH

ONE-POINT CALIBRATION

• Proceed as described in “two-point calibration” section.

• Press the CAL key after the first calibration point has been confirmed.

The instrument will return to normal operational mode and will memorize the
one-point calibration data.

10

ENHANCED CALIBRATION MESSAGES

The stored calibration history to used issue error and warning messages during
calibration to help ensure the highest accuracy.

As electrode aging is normally a slow process, substantial changes from
previous calibrations are likely due to a temporary problem with the electrode
or buffers. Calibrating under these conditions will give measurement errors.

ERROR MESSAGES

Error messages appear if one or all of the calibration parameters are out of
accepted windows. When these messages are displayed calibration cannot be
confirmed.

WRONG BUFFER

This message appears when the difference between the pH reading and the
value of the selected buffer is too big. If this error message is displayed, check
if you have selected the proper calibration buffer.

CAL

WRONG

BUFFER

CLEAN ELECTRODE

This error message indicates a bad electrode condition (offset out of accepted
window, or slope under the accepted lower limit).

Clean the electrode according to the Cleaning Procedure on page 31 to
improve its condition and repeat the calibration. This ensures the removal of
film, dirt or deposits on the glass bulb and reference junction.

pH

ATC

BUFFER 1

CAL

ELECTRODECLEAN

pH

ATC

BUFFER 1

CHECK ELECTRODE alternating with CHECK BUFFER
This error message appears when electrode slope exceeds the highest accepted

slope limit. You should check your electrode and use fresh buffer.

CHECK

CAL

ELECTRODE

BUFFER

11

pH

ATC

BUFFER 1

ELECTRODE

This message appears if the cleaning procedure performed as a result of the
above two messages is found by the instrument to be unsuccessful. Replace the
electrode.

CAL

ELECTRODE

pH

ATC

BUFFER 1

WRONG BUFFER TEMPERATURE

This message appears if the temperature of the buffer is outside the defined
buffer temperature range.

CAL

WRONG

BUFFER

ATC

pH

WARNING MESSAGES

During calibration, the Calibration Check feature analyzes the electrode calibration
history and warns the user when problems have been detected. It is possible to
over ride the warning messages and confirm the calibration but it is not
recommended.

CLEAR CAL IF NEW ELECTRODE

This warning is displayed any time the new calibration parameters are better
than the previous parameters. You can clear the calibration history by press­ing the CLR key, or continue by pressing the CAL key.

CAL

CLEAR CAL if new electrode

pH

ATC

BUFFER 1

CLEAN ELECTRODE

This warning appears during Calibration Check for the second calibration
buffer when the instrument has detected a small variation of offset or both
offset and slope parameters. This variation may result from dirt on the
electrode. Refer to the electrode cleaning procedure. This ensures the removal
of film, dirt or deposits on the glass bulb and reference junction.

CFM

CAL

ELECTRODECLEAN

pH

ATC

BUFFER 1

CLEAN ELECTRODE alternating with CHECK BUFFER
This warning appears during Calibration Check in the first calibration buffer as

12