Eppendorf XplorerPlus Adjustment User Manual
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Eppendorf Xplorer®/Xplorer® plus
Adjustment
Copyright© 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior
permission of the copyright owner.
®
Eppendorf and the eppendorf logo, epT.I.P.S.
and Eppendorf Xplorer® are registered trademarks of
Eppendorf AG.
®
Trademarks are not marked in all cases with ™ or
in this manual.
Further information can be found on the enclosed CD or on our website
www.eppendorf.com.
Adjustment - en-02/042013
Table of contents
Eppendorf Xplorer
®
/Xplorer® plus
English (EN)
Table of contents
1 General information on adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2 Change adjustment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1 Pswrd. protection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2 Factory settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.2.1 Reset to the factory setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.3 Liquid type ethanol 75% . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.4 Liquid type glycerol 50% . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.5 epT.I.P.S. long . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.6 Altitude . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.7 User adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.7.1 1-point adjust. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.7.2 2-point adjust. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.7.3 3-point adjust. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
2.8 Marking the Xplorer pipette to show the adjustment has been changed. . . . . . . . . . . . . . . . . . 17
3
3 Error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.1 Limiting values for measurement deviations according to ISO 8655 ddd . . . . . . . . . . . . . . . . . 18
3.1.1 Xplorer pipette, single-channel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.1.2 Xplorer pipette, multi-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.2 Eppendorf AG limiting values for measurement deviations . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.2.1 Xplorer pipette, single-channel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.2.2 Xplorer pipette, multi-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4 Speed table Eppendorf Xplorer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.1 Speed table for Xplorer single-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.2 Speed table for Xplorer multi-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5 Volume limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
6 Additional volumes during aspiration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
7 Patents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Table of contents
Eppendorf Xplorer
4
English (EN)
®
/Xplorer® plus
General information on adjustment
Eppendorf Xplorer
®
/Xplorer® plus
English (EN)
1 General information on adjustment
This operating manual applies to devices with software version 2.01.00 or higher.
The piston stroke of Xplorer pipettes can be changed using the Adjustment option. This document provides
information on when the Factory settings adjustment should be replaced by a different adjustment and what
items need to be observed during this process. A selected adjustment can be changed again at any time.
The adjustment can also be reset Factory settings at any time.
If the Xplorer adjustment Factory settings is not valid, this will be indicated using the symbol at the top
left of the header of the display.
NOTICE! Incorrect dispensing volume with special liquids and from temperature
differences.
Solutions which differ greatly from water in terms of their physical data, or temperature
differences between the pipette, pipette tip and liquid, can result in incorrect dispensing
volumes.
5
Avoid temperature differences between pipette, pipette tip and liquid.
Check the dispensing volume and make sure that you can affirm all the questions listed in
the general information.
The random and systematic errors determined before delivery can be found in the enclosed
Eppendorf Certificate. If the factory settings are superseded by a different adjustment, the
Eppendorf Certificate is no longer valid. If you reset the adjustment to the factory settings,
the random and systematic errors determined before delivery and the enclosed Eppendorf
Certificate are once again valid.
The Xplorer pipette was calibrated before delivery and tested with double distilled water according to ISO
8655.
Changing the adjustment of the Xplorer pipette is sometimes recommended for solutions with a density,
viscosity, surface tension and/or vapor pressure that vary greatly from that of water If the density of an
aqueous solution changes by approximately ±10%, e.g., due to the salt concentration, the volume changes
by approximately ±0.2%. This statement does not apply if other relevant properties of the liquid also
change.
If the pipette is used at extremely high altitudes, it must be calibrated to the ambient air pressure. At
1 000 m above sea level, the volume error of a 100 µL pipette is about –0.3%.
Changing the adjustment can improve the dispensing accuracy (systematic error) when using tips that have
a very different geometry than standard tips.
You can cancel a modified adjustment using simple steps.
Changes to the adjustment do not affect the precision (random error) of the dispensing. The precision
(random error) can be improved by replacing worn parts. Furthermore, handling has a strong influence on
the precision (random error).
General information on adjustment
Eppendorf Xplorer
6
®
/Xplorer® plus
English (EN)
Before the adjustment is changed, the existing adjustment must be checked in the factory settings using
distilled water.
The actual volume can be checked by weighing:
The density of distilled water is Approx. 0.9982 mg/µL at 20°C and 0.9965 mg/µL at 27°C.
If the set volume corresponds to the actual volume, no correction is necessary.
If there is a difference between the actual volume and the set volume of distilled water, please check the
following:
• Is there any liquid dripping from the tip?
• Is the pipette tip fitted leak-proof?
Adequate leak tightness is ensured when no drop is formed at the pipette tip after aspiration
of the nominal volume with distilled water and a waiting time of approx. 15 s. Hold the pipette
vertically, without touching the pipette tip. Prewet the pipette tip several times.
• Is the tip cone undamaged?
• Are the piston and the cylinder leak-proof?
• Does the temperature of the pipetted liquid correspond to:
– The temperature of the device?
– The ambient air temperature?
• Is the weighing location free from drafts?
• Does the work method and pipetting speed permit complete aspiration and dispensing of the liquid?
• Has the correct numerical value for "Density liquids at weighing temperature" been used for the
calculation of the actual volume?
• Is the set volume correct?
• For very small volumes (<10 µL): is the fine balance sufficiently sensitive (balance resolution:
0.001 mg)?
• Were original epT.I.P.S pipette tips used for testing?
The pipette tip which must be used as the test tip for checking technical data is listed in the
"Technical data" chapter in the operating manual.
No adjustment changes are allowed unless you can answer all the questions with "yes".
In all other cases, the problems associated with the questions answered with "no" must be eliminated. If the
problem is remedied by exchanging a complete lower part or other parts that have an effect on the volume,
proper assembly must be verified by carrying out a gravimetric test. The systematic and random errors
which must be met are listed in the technical data.
Change adjustment
Eppendorf Xplorer
If you would like to conduct a 1-3 point adjustment for a liquid other than water on the Xplorer, the items
listed above should also be precisely observed when determining the balance results. In particular, please
note that the density of the test liquid must be precisely determined at the test temperature. The density is
dependent on the temperature. The use of an incorrect density will lead to an incorrect volume calculation.
Furthermore, use the specifications in the Standard Operating Procedure for Pipettes
document on the Xplorer CD as the foundation for determining the weighing results
CD
and errors.
®
/Xplorer® plus
English (EN)
2 Change adjustment
Proceed as follows to perform an adjustment for special conditions, or to reset the adjustment to the factory
setting.
If you select the Factory setting adjustment the previously selected adjustment will be deleted.
This adjustment is not accompanied by a symbol appearing in the left half of the header in the
display.
Move the selection dial to Opt.
Select the Adjustment option with the rocker.
Press the Select softkey to open the Options.
The user has the following possibilities to change the adjustment:
7
• Factory settings
• Ethanol 75%
• Glycerol 50%
• epTIPS long
• Altitude
• 1-point adjust.
• 2-point adjust.
• 3-point adjust.
The adjustments Ethanol 75% or Glycerol 50%, epTIPS long and Altitude can be combined
with each other. Before routine use, check whether the combination of the adjustments meets
your requirements.
Use the rocker and the softkeys to activate the desired adjustment.
The adjustment is now active in all modes.
Turn the selection dial to any operating mode.
The adjustment has been activated successfully the symbol appears in the display. To the
right of the symbol, another symbol indicates the adjustment version.
Change adjustment
Eppendorf Xplorer
8
English (EN)
®
/Xplorer® plus
2.1 Pswrd. protection
Only available for the Xplorer plus pipette
For the Xplorer plus, the adjustment can be protected with a four-digit numeric password. The password
for the Edit mode and Adjustment option can vary. The password can be changed or deactivated after it has
been entered. Please contact Eppendorf AG Application Support or conduct a reset if you lose the
password.
The adjustment can no longer be changed if the password has been lost.
Press the rocker upward to activate the password protection.
ON appears in the display.
Press the Select softkey.
Use the rocker to select the digit.
Press the Next softkey to switch to the next field.
Enter data in all of the fields.
Press the Save softkey to save the password.
Reenter the password after completing the saving procedure. Press the Enter softkey.
Password protection is now active.
2.2 Factory settings
To aspirate liquid into a pipette tip, a piston is moved upwards in a cylinder in the Xplorer pipette. The
pipette tip is approximately conical in shape. This requires that the piston stroke in the cylinder be adapted
for aspiration in the conical pipette tip. The mathematical correction of the piston stroke is different for
each volume size of the Xplorer pipette. The respective correction of the piston stroke has been optimized
in the factory setting for the test tip specified in the technical data.
The factory setting is usually the best setting for all dispensing tasks with purely aqueous
solutions at room temperature.
When verifying the measurement deviations listed in the technical data, the factory setting
must be used with the test tip listed in the technical data.
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