Operating manual—Eppendorf Xplorer® Eppendorf Xplorer® plusseeon p.Fig.Tab.p
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perating manua
4861 910.011
Eppendorf Xplorer®
Eppendorf Xplorer® plus
Operating manual
Copyright© 2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of
the copyright owner.
®
eppendorf
, epT.I.P.S.® and Eppendorf Xplorer® are registered trademarks of EppendorfAG, Hamburg, Germany.
Trademarks are not marked in all cases with ™or ®in this manual.
Xplorer pipettes are using U.S. Patent Nos. 7,694,592 B2, 8,028,592 B2, 7,674,432 B2, 7,585,468 B2, 7,434,484 B2,
6,778,917, 6,499,365.
Further information can be found on the enclosed CD or on our website
www.eppendorf.com.
4861 910.011-00/022012
Eppendorf Xplorer® (plus) — Operating manual
Table of contents
1 Table of contents
1
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.2.1 Hazard icons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.4 Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.1 Single-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.2 Multi-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.2 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.2.1 Header . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.2.2 Status line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.2.3 Main field . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.2.4 Footer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.3 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.4 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.5 Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.6 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
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4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.1 Power supply assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.2 Connect rechargeable lithium-polymer battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.3 Charging the rechargeable battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.1 Switching the pipette on and off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.2 Setting the Date and Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.3 Principle of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.4 Selecting the operating mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.5 Setting the parameter (edit mode) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.6 Using pipette tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.7 Tips for correct pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.7.1 Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.7.2 Aspirating liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.7.3 Dispensing liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.8 Automatic dispensing (Ads dial setting) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.9 Dispensing (Dis dial setting) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.10 Pipetting (Pip dial setting). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.10.1 Standard pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.10.2 Reverse pipetting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.11 Pipetting and mixing (P/M dial setting) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.12 Manual pipetting (Man dial setting). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.13 Special operating modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.13.1 Multiple aspiration (Aspirate) (Spc dial setting) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
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5.14 Program (Prg dial setting). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
5.15 Editing (Edit dial setting). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
5.16 Pipetting with fixed volume (Fix dial setting) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
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5.17 Option (Opt dial setting) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
5.13.2 Diluting (Spc dial setting) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
5.13.3 Sequential dispensing (Spc dial setting) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.13.4 Reverse pipetting (Spc dial setting) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
5.13.5 Sequential pipetting (Spc dial setting) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
5.15.1 Pswrd. protection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
5.15.2 Fixed volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
5.15.3 Programm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
5.17.1 General help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
5.17.2 Volume limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
5.17.3 Counter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
5.17.4 Ejector reset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
5.17.5 History . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
5.17.6 Rocker setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
5.17.7 Sound level . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
5.17.8 Brightness . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
5.17.9 Adjustment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
5.17.10 Language . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
5.17.11 Personalization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
5.17.12 Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
5.17.13 Service interval . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
5.17.14 Date and Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
6 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
6.1 Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
6.2 Error search . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
7.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
7.2 Sterilizing or disinfecting the pipette . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
7.2.1 Autoclaving. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
7.2.2 Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
7.3 Replacing O-rings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
7.3.1 Removing the O-ring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.3.2 Mounting a new O-ring. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.4 Xplorer pipette disassembly and assembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.4.1 Single-channel up to 1000L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.4.2 5mL and 10mL single-channel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
7.4.3 Multi-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
7.5 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
8 General information on adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
9.1 Eppendorf Xplorer single-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
9.2 Eppendorf Xplorer multi-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
9.3 Test conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
9.4 Speed table for Xplorer single-channel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
9.5 Speed table for Xplorer multi-channel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . 66
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10 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
11 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
11.1 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
11.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
11.3 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
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Table of contents
5
Eppendorf Xplorer® (plus) — Operating manual
1 Operating instructions
1 Operating instructions
1.1 Using this manual
1
1.2 Danger symbols and danger levels
1.2.1 Hazard icons
Operating instructions
1.2.2 Degrees of danger
Read this operating manual completely before using the device for the first time. Also observe
the instructions for use of the accessories.
This operating manual is part of the product. thus, must always be easily accessible.
Enclose this operating manual when transferring the device to third parties.
If this manual is lost, please request another one. The current version is available on our
website:
www.eppendorf.com.
This operating manual applies to devices with software version 2.01.00 or higher.
The operating manual in additional languages, adjusting instructions and additional
information can be found on the Xplorer CD or on our website
Biohazard Explosion
Electric shock Toxic substances
Hazard point Material damage
The safety instructions in this operating manual indicate the following degrees of danger:
www.eppendorf.com.
1.3 Symbols used
DANGER Will lead to severe injuries or death.
WARNING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
Depiction Meaning
1.
2.
•
Text Terms and key names from the software.
You are requested to perform an action.
Perform these actions in the sequence described.
List.
References useful information.
6
Eppendorf Xplorer® (plus) — Operating manual
1.4 Glossary
A
Air-cushion
principle
Biopur Eppendorf Biopur® is an EppendorfAG purity level for consumables. In addition to the
Blow-out Movement of the piston that dispenses the remaining liquid in the pipette tip after the pipetting
Cycle Together, the piston movement upward (liquid aspiration) and piston movement downward (liquid
Free jet dispensing Dispensing steps without a pipette tip touching tube wall. If a drip forms on the pipette tip after
Increment Step size or resolution. For example, the volume can be increased or decreased in 0.01mL
ISO 8655 The ISO8655 standard defines, among other factors, limiting values (systematic error [accuracy]
Multivolume The total of the remaining stroke and the reverse stroke.
Nominal volume The maximum dispensing volume of a pipette. The nominal volume is also used as the name of a
PCR clean PCR clean is an EppendorfAG purity level for consumables. In addition to the requirements for
PhysioCare
Concept®
An air cushion is located between the piston-cylinder system of the pipette and the liquid in the
pipette tip. Moving the piston creates a vacuum and may lead to liquid being aspirated into the
pipette tip. During the downward movement of the piston, the air cushion is pressed downwards
and the liquid in the pipette tip is dispensed. Optimizing the piston stroke ensures that the stroke
volume leads to the liquid volume shown in the display.
Pipettes in which the piston is brought into contact with the liquid to be dispensed are defined as
positive-displacement pipettes.
B
requirements for standard goods, e.g., precision, accuracy, wetting behavior and leak tightness,
Biopur products also meet the highest standards when it comes to sterility and being free of ATP,
PCR inhibitors, human and bacterial DNA, pyrogens, DNase and RNase.
Goods with the Biopur purity level are inspected and certified by an external laboratory.
Lot-specific certificates can be downloaded from our website:
step has been completed. When using normal pipetting, the liquid blow-out is part of the
dispensing volume. When using reverse pipetting, blow-out is not part of the dispensing volume.
www.eppendorf.com.
C
dispensing) form a cycle.
F
free jet dispensing, this drip always belongs to the next dispensing step. We recommend
completing a dispensing series using only the free jet method or wall dispensing method.
Complete the reverse stroke in accordance with the dispensing series using either the free jet or
wall dispensing method. Errors listed in the technical data were determined using the wall
dispensing method.
I
increments for the volume selection on a 10mL Xplorer pipette.
and random error [precision]) and the test procedure for dispensing devices.
M
N
pipette. The term "nominal volume" comes from the ISO8655 standard.
P
standard goods, e.g., precision, accuracy, wetting behavior and leak tightness, products with the
PCR clean purity level also meet the highest standards when it comes to sterility and being free
of human DNA, DNase, RNase and PCR inhibitors.
Goods with the PCR clean purity level are inspected and certified by an external laboratory.
Lot-specific certificates can be downloaded from our website:
Products that carry the Eppendorf PhysioCare Concept logo feature optimized ergonomics and
make operation as simple as possible, even with complex dispensing tasks. PhysioCare products
can be easily serviced and cleaned. They are made of extremely light materials and require
minimal operating force.
www.eppendorf.com.
1
Operating instructions
7
Eppendorf Xplorer® (plus) — Operating manual
Rechargeable
lithium-polymer
1
Operating instructions
Remaining stroke After dispensing steps have been completed, any small quantities of residual liquid as safety
Reverse stroke After aspiration, the piston is moved into a defined position. Liquid is dispensed during this piston
Vapor pressure This term refers to the pressure exerted by the vapor of a material (solid or liquid) in an enclosed
Viscosity Viscosity describes the viscidity of liquids and suspensions. Dynamic or absolute viscosity is now
Wall dispensing Liquid dispensing during which the pipette tip touches the tube inner wall. We recommend
R
This rechargeable battery is a special version of the rechargeable lithium-ion battery. Like
rechargeable lithium-ion batteries, rechargeable lithium-polymer-batteries have a very high
battery
Sterile Sterile is an EppendorfAG purity level for consumables. In addition to the requirements for
charging capacity and a long service life. The special encapsulation of the rechargeable
lithium-polymer-battery makes it lighter than rechargeable lithium-ion batteries.
reserves; this liquid will be dispensed as the remaining stroke. You can discard the liquid of the
remaining stroke or reuse it.
movement. The reverse stroke is not a dispensing step.
S
standard goods, e.g., precision, accuracy, wetting behavior and leak tightness, products with the
Sterile purity level also meet the highest standards when it comes to sterility and being
pyrogen-free.
V
container. The vapor is in equilibrium with the solid or liquid phase of the material. The vapor
pressure increases when the temperature increases. Each pure liquid has a vapor pressure of
1013hPa (mbar) at boiling point. Volume errors caused by high vapor pressure can be reduced
by prewetting the tip.
usually given in Pa·s or mPa·s. The unitP orcP is frequently found in older literature.
1mPa·s corresponds to 1cP.
At room temperature a 50% glycerin solution has a viscosity of approx. 6mPa·s. As the glycerin
concentration increases, viscosity increases considerably. Absolutely anhydrous glycerin has a
viscosity of approx. 1480mPa·s at room temperature.
W
completing a dispensing series using only the free jet method or wall dispensing method.
8
Dis
P/M
Man
Opt
Spc
Prg
Edit
Fix
Ads
Eppendorf Xplorer® (plus) — Operating manual
2 Product description
2 Product description
2.1 Main illustration
2.1.1 Single-channel
1
2
3
Fix
Ads
Edit
Dis
Prg
Pip
Spc
P/M
Opt
Man
4
5
6
1 USB port
Only for Eppendorf service.
2 ON switch
3 Reset key
4 Connector socket
For connecting the charging plug of an Eppendorf Xplorer power
2
supply.
7
8
5 Selection dial
For setting an operating mode.
6 Charging contacts
Product description
Gold-plated contacts for loading and storage in a series 4880
9
10
charger stand or charger carousel.
7 Backlit color display
Switch the display off when it is not in use. The display
automatically switches back on when the pipette is moved.
8 Softkeys
The assignment of the respective softkey is shown in the display
footer.
9 Rocker
You can either press the rocker up or down. The rocker can
either be used to trigger the piston movement or to scroll through
12345
11
the menu and change the values of parameters. The color of the
rocker is different for the various volumes and matches the color
of the trays for the suitable epT.I.P.S. pipette tips.
12
13
10 Ejector
Moves the ejector sleeve and ejects the pipette tip. After a filled
pipette tip has been ejected, the piston returns to the basic
position after a slight delay. This option can be switched off
(see p. 42).
14
11 Serial number
12 Ejector sleeve
The ejector sleeve is marked with the nominal volume of the
pipette.
13 Spring-loaded tip cone
The spring loading action optimizes the force required for
attaching and ejecting tips. The spring loading action is available
for all Xplorer pipettes up to 1000L.
14 Pipette tip
The Xplorer pipettes may only be used with the suitable pipette
tip fitted. We recommend using epT.I.P.S.
9
Eppendorf Xplorer® (plus) — Operating manual
2.1.2 Multi-channel
2
Product description
A description of the upper part can be found in
the main illustration of the single-channel pipette
(see p. 9).
1Lever
The lever is used to release the multi-channel
lower part.
2 Multi-channel lower part
The multi-channel lower part is autoclavable.
The multi-channel lower part is freely rotatable. It
is not detached when rotated. The outer channels
are marked with the numbers 1 and 8 (or 12).
The multi-channel version has a piston for each
channel so that less than 8 or 12 tips can also be
mounted.
You can open the multi-channel lower part in
6
1
2
3
4
5
order to exchange or remove individual channels.
3 Latches, right and left
Unlock the cover plate(6) and internal ejector rail.
The 1200L multi-channel lower part can be
opened with a pin (see p. 58).
4 Spring-loaded tip cones for 10L, 100L and
300L
The spring loading action optimizes the force
required for attaching and ejecting tips.
5 Pipette tips
We recommend using epT.I.P.S.
6 Cover plate for 10L, 100L and 300L
Removable cover plate with internal ejector rail.
The cover plate is opposite the side with the
lever(1).
2.2 Display
10
The brightness of the display can be changed in the options (see p. 42). The display dims during
usage intervals and switches off after a prolonged usage interval. Move the Xplorer pipette to
switch the display back on.
1
2
3
4
1 Header 2 Status line
3 Main field 4 Footer
Eppendorf Xplorer® (plus) — Operating manual
2.2.1 Header
Adjustment
The adjustment can be changed in the options. The adjustment is described separately
Hint!
(see p. 61). Detailed information can be found in a separate document on the CD and on our
website:
If you have changed the factory settings, the symbol will be displayed to the left of the header.
Additional symbols to the right indicate which adjustment is selected in the options.
1-point adjust.
Single-point adjustment by the user.
2-point adjust.
Two-point adjustment by the user.
3-point adjust.
Three-point adjustment by the user.
Glycerol 50%
Adjustment to liquid type 50%glycerol.
Ethanol 75%
Adjustment to liquid type 75%ethanol.
Altitude
Adjustment to an altitude other than 0m above sea level.
epTIPS long
Adjustment to an epT.I.P.S.long.
www.eppendorf.com.
2
Product description
Volume
Battery
2.2.2 Status line
2.2.3 Main field
The selected volume for the acoustic feedback can be set in the options (see p. 42).
If the volume has been set to 0, no loudspeaker symbol is displayed.
Information on the charging capacity of the Li-polymer battery
Rechargeable battery fully charged.
Rechargeable battery partially discharged.
Rechargeable battery almost discharged. Rechargeable battery must be charged (see p. 17).
The selected operating mode is displayed here during operation. In edit mode, the name of the
parameter to be edited is displayed.
All parameters of the operating mode are displayed simultaneously during dispensing. A black
arrow indicates the direction of the next piston movement.
In edit mode, the parameter to be edited is marked in red.
Some of the dispensing parameters in the main field include:
• Dispensing volume
• Speed: 8 speeds for aspiration and dispensing
• Counter: Number of conducted dispensing, optional setting in the Pip mode
An explanation of all dispensing parameters can be found in the "Setting parameters" chapter
Hint!
(see p. 22).
11
Eppendorf Xplorer® (plus) — Operating manual
2.2.4 Footer
Assignment of the softkeys with variable functions.
Some of the softkeys in the footer are:
Edit Open the edit mode.
Next Select next parameter.
End Exit the edit or help mode.
Help Show help.
History Display the last dispensing settings of the accessed mode (see p. 44).
2
2.3 Delivery package
Product description
The right softkey can be assigned to Help or History. If the right Help softkey is activated, the
Hint!
softkey can be used to display information on the selected operating mode. If the History softkey
is activated, it can be used to call the most recently executed dispensings. The assignment of the
softkey can be defined in the options (Opt). The softkey is assigned to Help when the Xplorer is
delivered.
Quantity Description
1 Eppendorf Xplorer
1 Rechargeable lithium-polymer battery
1 Power supply with power plug adapters
1 English/German operating manual
Additional languages are available on the CD.
1 CD with operating manual in several languages, ordering information and
additional documents
1 Short instructions in English
Additional languages are available on the CD.
1 Locking ring for deactivating the spring loading action for 1000L
single-channel EppendorfXplorerpipettes
1 For multi-channel pipettes from 100L upwards:
multi-channel tool for cutting the O-rings on the tip cones
12
1 Unlocking tool for opening the 1200L multi-channel lower part
1 Pipette wrench for opening the lower part on 5mL and 10mL single-channel
pipettes
2 or 3 Locking clip for lifting the spring loading on 8 or 12 channel lower parts with
10L, 100L and 300L nominal volumes.
Eppendorf Xplorer® (plus) — Operating manual
2.4 Features
The Xplorer pipettes are motorized and electronically controlled piston-stroke pipettes designed
to aspirate and dispense liquid volumes according to the air-displacement principle. The Xplorer
pipette family includes single-channel and multi-channel pipettes (8 or 12 channels). Depending
on the Xplorer pipette, you can dispense volumes ranging from 0.5L to 10mL. The liquid is
aspirated into a pipette tip which is attached to the Xplorer pipette.
The main control element of the Xplorer pipette is a rocker. The color of the rocker differs
depending on the volume range of the Xplorer pipette. It has different functions during dispensing
operations and in the dispensing parameter editing mode. The rocker can either be pressed up or
down.
The lower parts of the Xplorer pipettes are autoclavable.
For all Xplorer pipettes from 10L to 1000L nominal volumes, the tip cone responds with
spring-loading action when a pipette tip is attached.
2
2.5 Warranty
2.6 Materials
NOTICE!
In case of warranty claims, contact your local Eppendorf contractual partner. No warranty is
provided for any damage due to misuse or if the upper part has been opened by unauthorized
persons. The rechargeable lithium-polymer battery and all other wear parts are excluded from the
warranty.
Aggressive substances may damage the pipette, pipette tips and accessories.
Check the chemical resistance before using organic solvents or aggressive chemicals.
Observe the cleaning instructions (see Cleaning on p. 51).
Components of the Xplorer pipette that can be accessed by the user are made of the following
materials:
Component Material
External surfaces of the upper part
Exterior and interior of lower parts
• Improved polypropylene(PP)
• Polycarbonate(PC)
• Polycarbonate(PC), coated
• Polycarbonate(PC), dyed
• Gold plated
• Improved polypropylene(PP)
• Polyvinylidene fluoride(PVDF)
• Polyetherimide(PEI)
• Polyphenylene sulfide(PPS)
• Polyetheretherketone(PEEK)
• Polytetrafluoroethylene(PTFE)
• Ethylene propylene diene rubber(EPDM)
• Silicone
• Steel (stainless steel and spring steel)
Product description
Pipette tip Material
epT.I.P.S. Polypropylene(PP)
epDualfilter T.I.P.S. filter Polyethylene(PE)
13
3
Eppendorf Xplorer® (plus) — Operating manual
3 Safety
3 Safety
3.1 Intended use
The Xplorer pipette is a lab device intended for dispensing liquids in the volume range from
0.5L to 10mL, in combination with matching pipette tips. In vivo applications (applications in or
on the human body) are not permitted.
The Xplorer pipette may only be operated by trained specialist staff. All users must have read the
operating manual carefully and familiarized themselves with the device's mode of operation.
3.2 Warnings for intended use
Risk of explosion.
Do not operate the device in areas where work is completed with explosive substances.
DANGER!
Do not use this device to process any explosive or highly reactive substances.
Do not use this device to process any substances which could create an explosive
atmosphere.
Safety
WARNING!
WARNING!
WARNING!
Damage to health due to handling infectious liquids and pathogenic germs.
When handling infectious liquids and pathogenic germs, observe the national regulations, the
biological security level of your laboratory and the material safety data sheets and the
manufacturer's application notes.
Wear personal protective equipment.
Follow the instructions regarding hygiene, cleaning and decontamination.
For complete instructions regarding the handling of germs or biological material of risk group
II or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health
Organization, current edition of the Laboratory Biosafety Manual).
Damage to health due to toxic, radioactive or aggressive chemicals.
Wear personal protective equipment (PPE).
Observe the national regulations for handling these substances.
Observe the material safety data sheets and the manufacturer's application notes.
Personal injury caused by the incorrect handling of the rechargeable battery.
Do not disassemble or modify the rechargeable battery.
Never pierce, crush or throw the rechargeable battery.
Only use the rechargeable battery in the Xplorer pipette.
Do not touch leaky rechargeable batteries.
Do not use any damaged rechargeable batteries.
Dispose of damaged rechargeable batteries in accordance with legal regulations.
14
CAUTION!
Danger to people from careless use.
Never point the opening of a Xplorer pipette fitted with pipette tip at yourself or anyone else.
Only initiate dispensing if it is safe to do so.
For all dispensing tasks, make sure that you are not endangering yourself or anyone else.
Eppendorf Xplorer® (plus) — Operating manual
Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf may
CAUTION!
NOTICE!
NOTICE!
impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or
accept any liability for damage resulting from the use of incorrect or non-recommended
accessories and spare parts or from the improper use of such equipment.
Only use accessories and original spare parts recommended by Eppendorf.
Damage to device from missing pipette tips.
Only use the Xplorer pipette with fitted pipette tips.
Carry-over, contamination and incorrect dispensing results due to the incorrect use of
pipette tips.
The pipette tips are for single use only. Prolonged use can have a negative impact on dispensing
tasks.
Use the pipette tips only once.
Do not use autoclaved ep Dualfilter T.I.P.S. for dispensing.
3
Safety
Incorrect dispensing volume with special liquids and from temperature differences.
Solutions which differ greatly from water in terms of their physical data, or temperature
NOTICE!
NOTICE!
3.3 Information on product liability
differences between the pipette, pipette tip and liquid, can result in incorrect dispensing volumes.
Avoid temperature differences between pipette, pipette tip and liquid.
Check the dispensing volume and make sure that you can affirm all the questions listed in the
general information.
Damage to device from penetration of liquids.
Do not deposit the Xplorer pipette with a filled pipette tip.
Do not allow any liquids to penetrate the inside of the housing.
If liquid has penetrated the inside of the housing, have the upper part of the Xplorer pipette
repaired only by service partners of Eppendorf AG. Contact your responsible sales office
before returning a product.
In the following cases, the designated protection of the device may be compromised. Liability for
any resulting property damage or personal injury is then transferred to the operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by
Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
15
Eppendorf Xplorer® (plus) — Operating manual
4 Installation
4 Installation
4.1 Power supply assembly
4
1
a
b
c
IIIIIII
I.T.E. POWER SUPPLY
MODEL NO:
INPUT: 100–240 V 50/60 Hz 0.5 A
OUTPUT: 5 V = 1.0 A
Approval no.
N 1388 LEI-4 MADE IN CHINA
U
L
G
S
IIIIIIIIIIIIIIIIIIII
IIIIIIIIIIIIIIIIIIII
C
23
Fit the power plug adapter required for your power supply into the opening of the power
supply. In the event of any doubt which power plug adapter should be used for the power
supply unit, you should ask an electrician for advice.
4.2 Connect rechargeable lithium-polymer battery
The lithium polymer battery is already inserted in the battery compartment upon delivery.
1 Power plug adapters
a EU
d
b United Kingdom
c USA
d Australia
2 Power supply
3 Charging plug
1. Open the rechargeable battery compartment
cover.
Installation
2. Connect the plug of the rechargeable lithium
polymer battery to the connector socket.
3. Close the rechargeable battery compartment
cover.
On the Xplorer plus pipette, you will then be
prompted to set the date and time
(see p. 19).
16
Eppendorf Xplorer® (plus) — Operating manual
4.3 Charging the rechargeable battery
Incorrect or damaged power supplies can lead to severe person injury or damage to the
device.
WARNING!
NOTICE!
Incorrect or damaged power supplies can cause electric shock or cause the device to overheat,
catch fire, melt, short circuit or cause similar damage.
Only use the supplied power supply for charging the device. The correct power supply unit is
indicated via the Eppendorf logo and the device name on the power supply.
Do not use a damaged power supply.
Loss of full rechargeable battery charging capacity if charged incorrectly.
The supplied rechargeable battery is not charged completely. If the rechargeable battery is not
charged completely before it is used for the first time, its charging capacity will remain
permanently greatly reduced. The rechargeable battery will only achieve its full capacity after a
few discharge/charge cycles.
Charge the rechargeable battery for at least three hours before it is used for the first time.
Do not charge the rechargeable battery in a hot location (>60°C).
Only charge the rechargeable battery using the supplied power supply.
For identification purposes, the power supply contains a sticker with the name of the compatible
Hint!
devices. The power supply is designed for the 100V-240V range.
4986 603.005
Multipette
Repeater
Xplorer
Recharging the rechargeable battery
1. Insert the assembled power supply (order number 4986603.005) into a power outlet.
2. In the Xplorer pipette check whether a rechargeable battery is inserted and connected.
3. Insert the charging plug of the power supply into the connector socket of the Xplorer pipette.
The display briefly shows the connection with the power supply. The charging process is
indicated by a flashing rechargeable battery symbol in the display. If the rechargeable battery
is almost completely discharged, the display will only switch on after a slight delay.
4. Switch on the ON switch to observe the charging process.
The pipette can be charged in both switch settings.
5. Charge the rechargeable battery for at least three hours.
The rechargeable battery will only achieve its full capacity after several discharge/charge
cycles.
®
stream/Xstream
®
stream/Xstream
TM
4
Installation
17
Eppendorf Xplorer® (plus) — Operating manual
A 4880 series charger stand or charger carousel can also be used to store and charge
single-channel and multi-channel Xplorer pipettes (see Ordering information on the Xplorer
CD
CD).
4
Installation
4880 charging stand 4880 charging carousel
18
Eppendorf Xplorer® (plus) — Operating manual
5 Operation
5 Operation
5
5.1 Switching the pipette on and off
When not in use, the switched on pipette automatically switches to the standby mode after a
Hint!
On
On
period of time. The pipette automatically switches back on when it is moved.
Power on
Switch on the ON switch to turn on the pipette.
Power off
Switch off the ON switch to turn off the pipette.
5.2 Setting the Date and Time
Only available for the Xplorer plus pipettes
Set the Date and Time parameters during the initial start-up.
Requirement
• Battery connected (see p. 16).
• Battery charged (see p. 17).
• Pipette switched on (see p. 19).
Operation
1. Select Date or Time using the rocker.
2. Press the Select softkey.
3. Change the value using the rocker.
4. Press the Next softkey to change the field.
5. Press the End softkey to end the entry.
6. Change the second parameter in the same way.
7. Press the End softkey to end the entry.
To change the date and time at a later time, change the Date and Time parameter in the options
Hint!
(see p. 48).
19
5
Operation
Eppendorf Xplorer® (plus) — Operating manual
5.3 Principle of operation
Every Xplorer pipette is equipped with various operating modes and a primary mode for
configuring the Xplorer pipette. The adjustable parameters and operational procedures differ in
the operating modes.
During operation, the piston in the pipette is moved according to how the rocker is actuated.
When you press the rocker up, the piston in the pipette moves up. Liquid is aspirated into the
attached pipette tip. When you press the rocker down, the piston in the pipette moves down. The
liquid is dispensed into the pipette tip.
Press the Edit softkey to open the edit mode of the selected operating mode. Here, you can use
the rocker to change the parameter that is outlined and marked red.
Special operating sequences with an electric pipette are possible with dispensing with the rocker.
Hint!
For example, you can cancel a piston movement in the Dis, Pip and P/M modes at any time by
pressing the rocker in the other direction. You can cancel the piston movement in the Ads and
Man modes by releasing the depressed rocker. After the piston has been stopped, you can
continue the aspiration or dispensing process by pressing the rocker in the corresponding
direction.
If the piston is in the basic position, you can repeat the blow-out (Blow) at any time by pressing
the rocker down.
The pipette software is available in several languages. To change the language, change the
Hint!
Language parameter in the options (see p. 47).
20
P/M
Man
Op
Spc
Prg
Edit
F
ix
A
ds
Dis
Eppendorf Xplorer® (plus) — Operating manual
5.4 Selecting the operating mode
You can set the desired operating mode using the selection dial.
The following operating modes are available:
Selection dial Mode Xplorer Xplorer plus
Ads Auto dispensing
Dispensing partial volumes in a defined
interval
5
Operation
Dis Dispensing
Dispensing uniform partial volumes
Pip Pipetting
Aspiration and dispensing of liquid
P/M Pipetting and Mixing
Pipetting with freely selected mixing volume
Man Manual pipetting
Aspiration and dispensing when the rocker
is held pressed down
Opt Options
Making settings and changing the
adjustment
Spc Multiple aspiration
Aspiration of uniform partial volumes and
combined dispensing (reverse dispensing)
Diluting
For diluting a sample or reagent
Sequential dispensing
Functions like dispensing mode with a fixed
sequence of various volumes
Reverse pipetting
Functions like the pipetting mode, but in
reverse order
Sequential pipetting
For creating pipetting series with various
volumes
Prg Program
Execute saved dispensings
Edit Editing
Procedure programs and pipettings with
fixed volumes
Fix Pipetting with fixed volumes
Procedure pipettings with fixed volumes.
ix
ds
Edit
Dis
Prg
Pip
Spc
t
P/M
Man
To select an operating mode, turn the selection dial to the selected mode.
21
5
Eppendorf Xplorer® (plus) — Operating manual
Only available for the Xplorer plus pipette
If you have selected the Spc operating mode:
1. Use the rocker to select the required operating mode from the list.
2. Press the Select softkey.
Operation
5.5 Setting the parameter (edit mode)
Requirement
• Pipette switched on (see p. 19)
• Operating mode selected (see p. 21)
Change the parameters as follows:
1. Press the Edit softkey to open the edit mode.
2. Press the Next softkey to go to the next dispensing parameter.
3. Change the dispensing parameter marked in the display with the rocker.
4. Press the End softkey to save the changes, to exit the edit mode and to continue with
dispensing.
If you want to cancel editing without saving the changes made, briefly turn the selection dial to
Hint!
Hint!
another mode.
You can change the Speed, Time, Cycles and Counter parameters during the dispensing.
Press the Edit softkey during the execution.
Set volume
Aspiration and dispensing volume.
You can change the speed that the Xplorer pipette uses to change the
volume under the Rocker setting parameter in the options.
22
Set max. volume
Maximum aspiration and dispensing volume.
The set value appears after editing in the display next to Max.
Set volume/step
Volume per dispensing step. The maximum number of aspiration
steps (Steps) will be displayed when the Set volume/step is changed.
Aspiration speed
Aspiration speed
8 levels are available. The higher the numerical value, the higher the
speed.
Aspiration speed will be automatically and approximately used for the
blow-out (Blow).
Eppendorf Xplorer® (plus) — Operating manual
Dispensing speed
Dispensing speed
8 levels are available. The higher the numerical value, the higher the
speed.
Dispensing speed will be automatically and approximately used for
the blow-out (Blow).
Set time
Interval between the dispensing step (0.1s to10s).
Number of steps
Number of dispensing steps
The available number of dispensing steps depends on the setting for
Set volume/step and is automatically set to the maximum number
when Set volume/step is edited.
Under steps in the "Sequential dispensing" mode, the selected
number of dispensing steps will be displayed to the right, and the
dispensing step for the displayed volume will be shown at the left.
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Operation
Set counter
The counter counts the number of dispensing operations performed.
The numerical value of the counter is increased by 1 when the piston
reaches the basic position after dispensing.
If the piston is in the basic position, you can alternatively set the
counter to 0 by turning the selection dial forward or backward.
You can switch the counter on and off in the Options.
Mixing volume
The mixing volume used after pipetting. If you change the pipetting
volume under Set volume, the mixing volume changes accordingly.
Mixing cycles
1 to 99 mixing cycles can be set for the mixing volume. The mixing
cycles are counted down to 0. If you keep the rocker pressed down
during mixing, after 0 has been reached, mixing is continued until the
rocker is released. If you press the rocker up during mixing, the mixing
process is stopped.
The mixing speed matches the selected speed for aspiration and
dispensing.
Set sample vol.
Volume of the sample or reagent
The maximum potential diluent volume will be automatically set if the
volume of the sample is changed.
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