Eppendorf® Vis Cuvettes
Instructions for use
Copyright ©2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced
without the prior permission of the copyright owner.
Eppendorf
BioPhotometer
Trademarks are not marked in all cases with ™ or
®
, the Eppendorf logo, Eppendorf BioSpectrometer®, and Eppendorf
®
are registered trademarks of Eppendorf AG, Hamburg, Germany.
®
in this manual.
0012 584.822-00/012013
Operating instructions
Eppendorf
®
Vis Cuvettes
English (EN)
Instructions for use Eppendorf® Vis Cuvettesseeon p.Fig.Tab.p.
English (EN)Instructions for use
1 Operating instructions
Before using the consumables for the first time, read these instructions for use and the
operating manual of the device that you use the consumables with. You can find the
current version of the operating manual on the Internet at
www.eppendorf.com/
worldwide. These instructions for use do not replace the device operating manual.
2 Product description
Eppendorf macro Vis Cuvettes and Eppendorf semi-micro Vis Cuvettes are plastic
cuvettes for single use.
• Photometric measurements: Eppendorf Vis Cuvettes are suitable for measuring at
wave lengths from 300 nm to 900 nm.
• Fluorimetric measurements: Eppendorf Vis Cuvettes are suitable for fluorimeters such
as the Eppendorf BioSpectrometer fluorescence where the emitted light is measured in
the direction of the incoming light. The cuvettes are not suitable for fluorimeters where
the emitted light is measured perpendicular to the incoming light.
• Eppendorf macro Vis Cuvettes are suitable for temperature control for kinetic
measurements.
• The direction of the light path is marked with an arrow.
• Recessed measuring windows to protect against scratches.
• Sorting by nest numbers: small deviations of the extinction values between the
cuvettes of one packaging unit.
3 Safety
3.1 Intended use
Eppendorf Vis Cuvettes are plastic cuvettes for single use. They are used for the
photometric and fluorimetric analysis of liquids. Eppendorf Vis Cuvettes are used in
training, routine and research laboratories in the life sciences, in industry and, chemistry.
In-vivo applications (applications in or on the human body) are not permitted.
The device may only be operated by skilled personnel who have been trained in the areas
mentioned above.
3.2 Warnings for intended use
3
WARNING! Damages to health due to infectious liquids and pathogenic
germs.
When handling infectious liquids and pathogenic germs, observe the national
regulations, the biological security level of your laboratory, the material safety
data sheets, and the manufacturer's application notes.
Wear personal protective equipment.
Consult the "Laboratory Biosafety Manual" (Source: World Health
Organization, Laboratory Biosafety Manual, as amended) for comprehensive
regulations on the handling of risk group II germs or biological materials).
Operation
4
Eppendorf
English (EN)
®
Vis Cuvettes
4 Operation
4.1 Inserting the cuvette
Prerequisites
• The cuvette is free from contamination from dust or finger prints and free from
scratches.
• Measuring volume in the cuvette is sufficient. Observe that the minimum measuring
volume has been reached.
• Measuring solution is free from particles and bubbles.
NOTICE! Material damage due to improper handling
The cuvette may fall if it is not handled properly. Liquid may spray out.
Store the cuvette in a cuvette stand.
NOTICE! Faulty measured values due to incorrect orientation of the cuvette.
Always insert the cuvette in the same direction within a series of
measurements. Do not rotate the cuvette 180° during a series of
measurements.
Do not touch the optical surfaces of the cuvette.
The direction of the light path is marked with an arrow along the upper edge.
Eppendorf BioPhotometer/Eppendorf BioSpectrometer
Position the cuvette in such a way that the measuring window points towards the light
path.
During insertion, press the cuvette all the way down against a slight resistance.
4.2 Temperature control for kinetic measurements
For an efficient temperature control of the measuring solution during kinetic
measurements, the contact surface of the cuvette to the cuvette shaft must be as large as
possible.
Use the Eppendorf macro Vis Cuvettes for kinetic measurements
You can find notes on the temperature control of the cuvettes in the Eppendorf
BioSpectrometer kinetic in the operating manual of the device.
Technical data
Eppendorf
5 Technical data
5.1 Dimensions
Eppendorf macro
Vis Cuvettes
Eppendorf semi-micro
Vis Cuvettes
Width 12.5 mm (0.49 in) 12.5 mm (0.49 in)
Depth 12.5 mm (0.49 in) 12.5 mm (0.49 in)
Height 45 mm (1.77 in) 45 mm (1.77 in)
Filling volume
Eppendorf BioSpectrometer,
1 000 μL – 4 500 μL 400 μL – 3 000 μL
Eppendorf BioPhotometer
(light path height 8,5 mm)
Devices with light path
2 500 μL – 4 500 μL 1 500 μL – 3 000 μL
height of 15 mm
5.2 Photometric properties
Abb. 5-1: Transmission of the water-filled Eppendorf Vis Cuvettes
100
90
80
70
60
Transmission (%)
50
40
30
20
10
210 230 250 270 290 310 330 350
Wavelength (nm)
Fig. 1: Transmission of the water-filled Eppendorf Vis Cuvettes
®
Vis Cuvettes
English (EN)
5
Eppendorf macro
Vis Cuvettes
Eppendorf semi-micro
Vis Cuvettes
Optical path length 10 mm 10 mm
Measuring window 10 mm × 35 mm 4.5 mm × 23 mm
Wavelength range 300 nm – 900 nm 300 nm – 900 nm
Cuvette blank scattering s ≤ 0.004 A at 320 nm s ≤ 0.004 A at 320 nm
Ordering Information
6
Eppendorf
®
Vis Cuvettes
English (EN)
5.3 Chemical resistance
Storage before use Protect from direct sunlight and UV light. Store in dry
conditions at room temperature.
Chemical resistance Resistant to ammonia, hydrofluoric acid 10 %, hexane,
isopropanol, caustic soda.
Not resistant to acetone, benzaldehyde, chloroform,
dioxane, dimethylformamide, acetic acid, ethylacetate,
nitric acid 65 %, hydrochloric acid 36 %.
All data on chemical resistance refer to short-term resistance, 30 min max. The storage of
these chemicals in the cuvettes must be tested by the user.
6 Ordering Information
Order no.
(International)
Order no.
(North America)
0030 079.345 0030079345
0030 079.353 0030079353
4308 078.006 940001102
Description
Eppendorf macro Vis Cuvettes
10 × 100 pieces
Eppendorf semi-micro Vis Cuvettes
10 × 100 pieces
Cuvette stand
for 16 cuvettes
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Eppendorf AG · Hamburg · Germany · Phone: +49 40 538 01-0
www.eppendorf.com
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