Eppendorf TwinTip-Holder User Manual

TwinTip-Holder for TransferMan NK 2

®

Montage-Anleitung · Mounting Instruction

TwinTip-Holder for TransferMan NK2

Inhaltsverzeichnis

1 Sicherheitshinweise . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

2 Übersicht . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

3 Komponenten . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Montagemöglichkeiten . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4

3 Montage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

3.1 Kapillarenausrichtung . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

3.2 Anschluss der Kontrolleinheit an das Steuerpult. . . . . . . . . . . . . . . . . . . . . . . . . 7

3.3 Anbau der Kontrolleinheit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

4 Steuerung des TwinTip-Holders über die Kontrolleinheit . . . . . . . . . . . . . . . 9

4.1 Rote Taste . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

4.2 Schwarze Taste . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

4.3 Ausrichtung der Kapillaren . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

5Technische Daten . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

6 Bestellinformationen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Contents

1 Safety precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

2 Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3 components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Mounting options. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3 Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.1 Capillary alignment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

3.2 Connecting the control unit to the control board . . . . . . . . . . . . . . . . . . . . . . . 17

3.3 Mounting the control unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4 Controlling the TwinTip-Holders with the control unit . . . . . . . . . . . . . . . . 19

4.1 Red button. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.2 Black button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.3 Aligning the capillaries. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

5Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

6Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

2

1 Safety precautions

Please read the next section carefully before you unpack the device and check to
see if it is complete.

• Follow the micromanipulator safety precautions!

• Follow the safety precautions for the connected injectors!

• Make sure that the control board of the micromanipulator is not lying on the joystick
when you connect and assemble the control unit!

• Connections should only be carried out without power supply!
Pull the mains plug!

• Incorrect handling can result in injury!

Ensure that the capillaries being used are not aimed at persons when the
injectors (i.e. CellTram, FemtoJet, etc.) are connected.
The capillaries can fly from the capillary holder where there is strong
pressure. Blowpipe effect!
Protect eyes with safety glasses!

• During mounting and dismantling please check without fail that the red and black
retainer for the capillary holder are in the position "Down"! See the figures in
Section 4.
In the "Up" position damage / or destruction of the electromechanics may quickly
result if the retainer is pressed down either by hand or other means.

Transfer

•

•

If the device is passed on to someone else, please include the instruction manual.

Disposal

In case the product is to be disposed of, the relevant legal regulations are to be
observed.
The disposal of electrical devices is regulated within the European Community by
national regulations based on EU Directive 2002/96/EC on waste electrical and
electronic equipment (WEEE).
According to these regulations, any devices supplied after 13.08.05 in the business­to-business sphere, to which this product is assigned, may no longer be disposed of
in municipal or domestic waste. They are marked with the following symbol to indicate
this.

As disposal regulations within the EU may vary from country to country, please
contact your supplier if necessary.

13

2 Overview

3 components

Control unit TwinTip-Holder Mounting angle

Mounting options

The TwinTip-Holder is mounted onto the TransferMan NK 2 installed on the left or right­hand side.

Left-hand side mounting or right-hand side

14

3 Mounting

The mounting examples are for a right-hand side mounted micromanipulator.

The X-head

in both positions

Screwing on the mounting angle

15

3 Mounting

Three mounting positions in the axial direction:

Optional variations:

Axial variability

16

Mounting

Mounted

TwinTip-Holder

Sideways and rotational

variability

3 Mounting

3.1 Capillary alignment

Sideways

alignment

Height adjustment

When inserting and fixing the universal capillary holder and when aligning the capillaries
the TwinTip-Holder must be handled carefully. If excessive force is used, e.g. leverage
via the universal capillary holder, damage / or destruction of the fine electromechanics
may result.

3.2 Connecting the control unit to the control board

17

3 Mounting

3.3 Mounting the control unit

Control unit,
mounted

Connecting the TwinTip-Holders

18

4 Controlling the TwinTip-Holder with the control unit

Pressing the buttons:

Change position between "Up" and "Down";

The current position can be visually checked.

4.1 Red button

Pos. "Up"

Pos. "Down"

On mounting, dismantling and insertion of the universal capillary holder, the retainer
must be in the "Down" position. Otherwise damage or destruction of the electro­mechanics may result.

19

4 Controlling the TwinTip-Holder with the control unit

4.2 Black button

Pos. "Up"

Pos. "Down"

On mounting, dismantling and insertion of the universal capillary holder, the retainer
must be in the "Down" position. Otherwise damage or destruction of the electro­mechanics may result.

4.3 Aligning the capillaries

1. Move the micromanipulator completely up.

2. Set the desired angle on the X-head of the micromanipulator.

3. Position the capillaries with the capillary holder,
align lengthwise and screw tight.

4. Set the rear capillary (in the example) to that of the front.

5. Set the sideways position of the front capillary to that of the rear.

6. Readjustments from 2. – 4.are also possible later.

20

5 Technical data

The TwinTip-Holder is designed for mounting on the
Eppendorf TransferMan

Capillary holder reception: twofold,

Power supply
(through micromanipulator): 14 – 16 V DC; < 5 W

Number of possible positions: 2 for each capillary holder position

Degree of contamination: II

Ambient temperature: + 5 °C to + 40 °C

Relative ambient humidity: 20 % – 80 %,

Weight: TwinTip-Holder: 130 g

Dimensions: TwinTip-Holder: 60 x 33 x 38 mm

Technical specifications subject to change!

®

NK 2 micromanipulator.

for capillary holders with 4 mm diameter

Condensation excluded

Control unit: 170 g

Control unit: 40 x 62 x 100 mm

21

6 Ordering information

Order number

USA / Canada International

TwinTip-Holder 920-00-776-7 5188 200.011

Mounting angle
with knurled-head screws

Knurled-head screw set,
consisting of:
knurled-head screws, 4 pieces
arched spring washers, 2 pieces
pressure rings, 2 pieces
compression springs, 2 pieces

920-00-806-2 5188 852.000

920-00-807-1 5177 320.007

22

Eppendorf Offices

ASEAN

Eppendorf AG
Regional Office in Malaysia
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com.my

AUSTRALIA / NEW ZEALAND

Eppendorf South Pacific Pty. Ltd.
Tel. [phone] Fax [phone]
E-mail: [e-mail]
Internet: www.eppendorf.com.au

AUSTRIA

Eppendorf AG
c/o Schott Austria
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com

BRAZIL

Eppendorf do Brasil Ltda.
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com.br

CANADA

Eppendorf Canada, Ltd.
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com

CHINA

Eppendorf AG
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.cn

FRANCE

EPPENDORF FRANCE S.A.R.L.
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.fr

GERMANY

Eppendorf Vertrieb
Deutschland GmbH
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.de

INDIA

Eppendorf India Limited
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.co.in

ITALY

Eppendorf s.r.l.
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.it

JAPAN

Eppendorf Japan Co. Ltd.
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.jp

NORDIC

Eppendorf Nordic Aps
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.dk

SPAIN

Eppendorf Ibérica S.L.
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.es

SWITZERLAND

Vaudaux-Eppendorf AG
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com

UNITED KINGDOM

Eppendorf UK Limited
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.co.uk

USA

Eppendorf North America
Tel. [phone] Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorfna.com

OTHER COUNTRIES

see:
www.eppendorf.com/worldwide

23

In touch with life

Your local distributor: www.eppendorf.com/worldwide

Eppendorf AG · 22331 Hamburg · Germany · Tel. [phone] · Fax [phone]

E-Mail: [e-mail]

Eppendorf North America, Inc. · One Cantiague Road, P.O. Box 1019

Westbury, N.Y. 11590-0207 USA · Tel. [phone] · Toll free phone [phone]

Fax [phone] · E-Mail: [e-mail]

Application Support

Europe, International: Tel. [phone] · E-Mail: [e-mail]

North America: Tel. [phone] ext. 2258 · E-Mail: [e-mail]

Asia, Pacific: Tel. [phone] · E-Mail: [e-mail]

eppendorf ist eine eingetragene Marke

B 5188 200 984-02/0606 · Printed in Germany