PowerPac™ Universal
Power Supply
Instruction Manual
Catalog Number
164-5070
Table of Contents
Page
Safety ...........................................................................................i
Section 1 Introduction ......................................................................................1
1.1 Overview.................................................................................................1
1.2 Unpacking...............................................................................................2
Section 2 Control Features.........................................................................4
Section 3 Setup and Operation ..................................................................5
3.1 Setup ......................................................................................................5
3.2 Manual Mode Operation .........................................................................7
3.3 Method Mode Operation
3.3.1 Create and Run a New Method .................................................8
3.3.2 Edit (or View) and Run a Saved Method ..................................11
3.3.3 Edit a Paused Run...................................................................13
Section 4 Operation with a Personal Computer and PDA ......................15
4.1 Operation with a Personal Computer ....................................................15
4.2 Operation with a PDA ...........................................................................15
4.2.1 Create, Edit and Transmit Methods .........................................15
4.2.2 Transmit Data..........................................................................17
Section 5 Maintenance and Troubleshooting..........................................19
5.1 Maintenance .........................................................................................19
5.2 Troubleshooting ....................................................................................19
5.2.1 Basic Troubleshooting .............................................................19
5.2.2 Power Failure Detection ..........................................................19
5.2.3 Rapid Resistance Change/No Load Detection .........................20
5.2.4 User Serviceable Error Messages ...........................................21
5.3 Replacing a Fuse..................................................................................23
5.4 Expediting Technical Support................................................................23
Appendix A Specifications...........................................................................24
Appendix B Warranty and Ordering Information ........................................25
List of Figures
1. Front View.
2. Rear View.
3. Front View with Legs in Lowered Position.
4. Front Panel.
5. Power Leads Connected Correctly.
6. Power Leads Connected Incorrectly.
7. Rear View Showing Fuse Drawer with Notches.
© 2013 Bio-Rad Laboratories, Inc. All rights reserved.
Safety
!
Caution/Warning
PowerPac power supplies use high output voltages that are electrically isolated from earth
ground to minimize the risk of electrical shock to the user. The following guidelines should
be observed and followed when using a PowerPac power supply.
PowerPac power supplies have been tested for operation at temperatures between 0° and
40°C, with relative humidity between 0 and 95% non-condensing. Operating the power
supply outside these conditions is not recommended by Bio-Rad and will void the warranty.
1. To ensure adequate cooling of the power supply, be sure that there is at least 6 cm
clearance around the power supply. Do not block the fan vent at the rear of the unit.
2. Always connect the power supply to a 3-prong, grounded AC outlet, using the
3-prong AC power cord provided with the power supply.
3. Bio-Rad electrophoresis cells have molded two-prong plugs that are inserted into the
power supply’s high voltage output jacks. These plugs have been EN 61010* certified
for safety compliance for use with PowerPac power supplies. Use of other plugs or
banana jacks is done at the user’s own risk and is not recommended by Bio-Rad.
When inserting and removing the molded two-prong plug, always grasp the plug by the
molded support at the rear of the plug. Do not grasp the individual prong ends.
4. Do not operate the power supply in extreme humidity (>95%) or where
condensation can short the internal electrical circuits of the power supply.
5. When taking the power supply into a cold room, the unit can be operated immediately.
However, when removing the power supply from the cold room, let the unit equilibrate
to room temperature for a minimum of 2 hours before using it.
6. Never connect a high voltage output lead to earth ground. This defeats the floating
electrical isolation of the power supply and exposes the user to potentially lethal high
voltages.
Important
This instrument is intended for laboratory use only.
This product conforms to the class A standards for Electromagnetic Emissions, intended for
laboratory equipment applications. It is possible that emissions from this product may
interfere with some sensitive appliances when placed nearby or on the same circuit as
those appliances. The user should be aware of this potential and take appropriate measures
to avoid interference.
Bio-Rad’s PowerPac power supplies are designed and certified to meet EN 61010* safety
standards. Certified products are safe to use when operated in accordance with the
instruction manual. This safety certification does not extend to electrophoresis cells or
accessories that are not EN 61010 certified, even when connected to this power supply.
This instrument should not be modified or altered in any way. Alteration of this instrument
will void the manufacturer’s warranty, void the EN 61010 certification, and create a potential
safety hazard for the user. Bio-Rad is not responsible for any injury or damage caused by
the use of this instrument for purposes other than those for which it is intended, or by
modifications of the instrument not performed by Bio-Rad or an authorized agent.
*EN 61010 is an internationally accepted electrical safety standard for laboratory instruments.
Section 1
Introduction
1.1 Overview
The PowerPac Universal power supply is designed to provide constant voltage,
current or power for a wide range of electrophoresis applications, including high
throughput electrophoresis with the Dodeca cells and electrophoretic blotting.
Output specifications:
Voltage: Adjustable from 10 to 500 volts (V) in 1 V increments
Current: Adjustable from 10 to 2500 mA in 1 mA increments.
Power: Adjustable from 1 to 500 Watts (W) in 1 W increments.
Output jacks: Four sets of output jacks are provided to facilitate connection
of up to 4 identical electrophoresis cells simultaneously.
abc
def
3
jklghi
mno
6
tuvpqrs
wxyz
8
9
0CE
stop/home
setup
12
45
7
EDIT
FRONT PANEL
OUTPUT JACKS
Fig. 1. Front view.
Fig. 2. Rear view.
1
The PowerPac Universal has the following features:
• Constant voltage, constant current, or constant power operation with automatic
crossover
• LCD screen displays all run parameters at once
• Programmable methods with up to nine steps
• Storage capacity for up to nine user defined methods
• Continuous, hour and volt-hour time modes
• Paused run editing
• Safety features: no-load, short circuit, rapid resistance change, ground leak,
and internal thermal protection
• User optional run completion after an AC power failure
• Adjustable LCD display contrast
• EN61010 international safety certification
• Input power 100-120/220-240 VAC, 50/60 Hz, auto-switching
• Four output terminals
• Stackable case with adjustable viewing angle via flip down legs (See Figure 3)
• Infrared port, for transmitting data to a personal computer or PDA
Optional:
• Data transfer software (DTS) for data file management on a PDA and PC
LEGS
(LOWERED POSITION)
Fig. 3. Front view with legs in lowered position.
2
1.2 Unpacking
When you receive your power supply, carefully inspect the container for any
damage which may have occurred in shipping. Severe damage to the container
may indicate damage to the power supply itself. If you suspect damage to the unit,
immediately file a claim with the carrier in accordance with their instructions before
contacting Bio-Rad Laboratories.
After unpacking the PowerPac Universal, remove the plastic film from the
translucent green top case. The plastic film may leave a residue. If so, clean with a
soft, damp cloth. Also remove the die-cut plastic film covering the display window.
Contents include:
• PowerPac Universal power supply
• Power cord
• Instruction manual
• Warranty card
• Declaration of conformity
If any part is missing or damaged, contact Bio-Rad Laboratories immediately.
3
Section 2
CLEAR ENTRY KEY
ALPHANUMERIC
KEYPAD
ARROW KEYS
SETUP KEY
SOFT KEYS
EDIT KEY
IR PORT
RUN/PAUSE KEY
Control features
Fig. 4. Front Panel.
Key Description
Run/Pause Starts or pauses a run. When paused, the run parameters for the
current or subsequent step(s) may be edited. The modified
method may be stored to permanent memory by pressing SAVE at
the end of the run.
Stop/Home Terminates the run in progress and displays the final run
parameters, or if no run is active, changes the display to the Home
screen.
Note: If a method has been edited during a run, SAVE must be
pressed at the end of the run to save the changes.
Setup Used to set the PowerPac Universal default settings such as:
power failure detection, rapid resistance change detection, no load
detection as well as clock, contrast and key chirp settings (see
Section 3.1).
EDIT Toggles soft key assignments between those used to set the run
mode (constant voltage, constant current or constant power), run
limits (voltage, current or power) and time mode (hours, volt-hours
or untimed).
Arrow Keys Used to scroll through method list or method protocol. An
asterisk (*) is used to identify the selected method or step.
CE Deletes alphanumeric characters from a parameter value or
CE
method name, or restores previous numeric value.
Alpha-numeric Used to enter parameter values and method names. When
keypad method names are entered, the manner in which keys are pressed
determines the characters entered and their placement. Rapid
repetitive strokes on a single key cause the character displayed at
the cursor position to toggle between those associated with the
key (i.e. a®b®c®2®A®B®C®2). The cursor position advances
each time a different key is pressed or when there is a pause
between strokes of a single key.
Soft Keys The commands on the LCD screen immediately above the soft
keys assign their functionality.
4
Section 3
Universal
Setup and Operation
3.1 SET UP
This section describes how the PowerPac Universal power supply is set up and
connects to an electrophoresis cell(s).
Step Procedure Description
1.
Connect Cells Insert power leads into one of the output
terminals located on the front of the power
supply (shown to the left). Note that the
symbol indicates high voltages and that
the power leads must be inserted perpendicular to
the curve of the case (see Figure 5).
Fig. 5. Power Lead Connected Correctly
Universal
Fig. 6. Power Leads Connected Incorrectly
2. Turn power on Use the switch located on the right side of the
power supply to turn the power on. When turned
FW Version: 1.20
Serial No. 000002
on, the unit briefly displays the firmware version
and serial number and then goes to the Home
screen. The Home screen soft keys are used to
operate the power supply in
manual (Section 3.2) or method mode (Section
3.3).
SELECT MODE
MANUAL mode: Program and run a single step
method without storing into memory.
MANUAL METHODS
METHODS mode: Program, store, and run
single or multistep methods.
3. Open the setup editor Press SETUP to start the setup editor. SETUP
can be pressed from any screen except the run
screen.
5
Step Procedure Description
!
!
4. Set the Power Failure Use the PFd soft key to set the Power Failure
detection mode detection mode:
SETUP
PFd = OFF: Turns power failure detection
off. In this mode, the run is terminated if a
PFd: OFF
RRCd-NLDd: ON
PFd RRCd-NLDd PANEL
power failure occurs.
PFd = ON NEXT RUN ONLY: Turns
power failure detection on for a single run.
PFd = ON: Turns power failure detection
mode on. If there is a power failure in this
mode, then the run will resume when the
power is restored after single or mulitple
power failures.
Warning: Turning the power supply off to stop a
run in progress is regarded as a power failure and
the interrupted run (when PFd = ON) will resume
the next time the power supply is turned ON.
5. Set the Rapid Press the RRCd-NLDd soft key to change the
Resistance Change Rapid Change detection and No Load
detection/No Load detection mode.
detection mode
RRCd-NLDd = ON: Detection of a load
SETUP
change greater than 20% or 15 mA, or the
absence of a load (current is less than 2
PFd: OFF
RRCd-NLDd: ON
PFd RRCd-NLDd PANEL
mA), will cause an alarm to sound and the
run to pause.
RRCd-NLDd = OFF: Does not detect
sudden load changes or the absence of a
load. When running in this mode, the
symbol is displayed on the Run
screen as a reminder that this safety
feature has been turned off.
Note: This mode is used to complete
electrophoresis application such as the D-Code
that require that Rapid Resistance Change
detection be turned off or applications such as
isoelectric focusing that require less than 2 mA
current.
6. Set the control panel Press the PANEL soft key.
default settings
7. Set the default contrast Press the CONTRAST soft key and enter a
value from the keypad. The contrast value range
Tu e
27-May-03
CONTRAST: 95
KEY CHIRP: ON
CLOCK CONTRAST CHIRP
11:22
is between 80 and 110.
6
Step Procedure Description
8. Set the key chirp mode Press the CHIRP soft key to turn key chirp on
or off.
Tu e
27-May-03
CONTRAST: 95
KEY CHIRP: ON
CLOCK CONTRAST CHIRP
11:22
KEY CHIRP = OFF: No sound is
produced when a key is pressed. This
does not affect alarms.
KEY CHIRP = ON: A chirp noise is
heard every time a key is pressed.
9. Set the clock Press the CLOCK soft key to display the clock
settings screen. Use the PREV and NEXT soft
27-May-03
Tu e
CHANGE DAY W/ARROWS.
CONTRAST: 95
KEY CHIRP: ON
PREV NEXT SET
11:22
keys to move the cursor between the day,
date and time fields. The up and down arrow
keys are used to set the day and month and
the keypad is used to enter the date and time
values.
Press the SET soft key to accept the new day,
date and time values.
Press Setup once to return to the previous
screen or twice to exit setup mode.
Note: The PowerPac Universal contains an
internal battery to maintain the clock settings
during power off.
3.2 Manual Mode Operation
This section describes how to program and perform a run in manual mode.
In the Manual mode the user can program and start a single step method with a
minimum number of keystrokes. This single step method is not stored in
permanent memory; however, the run parameters are kept in the flash memory
until the PowerPac is turned off. This allows repeating the same run without having
to re-enter the values.
Step Procedure Description
1. Set up the power supply Refer to Section 3.1 for instrument setup.
2. Select a run mode From the Home screen, press the MANUAL
soft key to perform a single step run in manual
SELECT MODE
MANUAL METHODS
mode or press METHODS to run a multi-step
method (see Section 3.3).
3. Select a constant Use the soft keys to select the constant
parameter parameter and the keypad to enter its value.
The constant parameter is displayed in large
EDIT CONSTANT
0 V
TIME: UNTIMED
CONSTV
CONSTA CONSTW
2500
500
mA
W
font. If the run will be performed as an
un-timed run at the displayed maximum limit
values for the non-constant parameters
(shown to the right of the constant parameter)
skip to Step 6, otherwise press the EDIT key.
ConstV: Used to run with constant voltage.
ConstA: Used to run with constant current.
ConstW: Used to run with constant power.
7
Step Procedure Description
4. Enter limits for the Use the soft keys to select a non-constant
non-constant parameter and the keypad to enter its value.
parameters If the run will be performed as an un-timed
run, skip to Step 6, otherwise, press the EDIT
EDIT LIMITS
100 V
TIME: UNTIMED
CONSTV
LIMITA
2500
500
LIMITW
mA
W
key. Note: to change the constant value, press
its soft key and enter a new value.
ConstV: Used to change the voltage
constant value.
LimitA: Used to change the maximum
default value for current limit.
LimitW: Used to change the maximum
default value for power limit.
5. Select the time mode Use the soft keys to select the time mode and
the keypad to enter the run time. Press the
EDIT TIME
100 V
TIME: 00:00 HRS
VHOURS
HRS
2500
500mAW
UNTIMED
EDIT key to return to Step 3, otherwise,
proceed to the next step.
HRS: Run time is in units of hours.
VHOURS: Run time is in units of volt-hours.
UNTIMED: Run is continuous.
6. Start the run Press to start a run.
7. Monitor the run The run screen is used to monitor run
parameters or to pause a run. The elapsed
RUN
100 V
00:00/01:00 HRS
525mA
W
PAUSE
time versus the programmed time, voltage,
current and power values may be viewed from
the run screen.
Press the PAUSE soft key to edit the run
parameters (see Section 3.3.3) or to abort the
run. Press to terminate the run.
3.3 Method Mode Operation
This section describes how to program and perform a run in Method mode. Method
mode operation is used to create and run single and multi-step methods. In
method mode, up to nine methods, each with up to nine steps may be
programmed, stored, or edited. Each of these methods may be transferred to or
from a PDA, through the PowerPac Universal IR port as described in Section 4.
3.3.1 Create and Run a New Method
This section describes how to create and run a new method.
Step Procedure Description
1. Setup the power supply Refer to Section 3.1 for instrument setup.
8
Step Procedure Description
2. Select the programming On the Home screen, press the METHODS
mode soft key to display the Method List screen.
SELECT MODE
MANUAL METHODS
3. Create a new method Press the NEW soft key on the Methods List
screen to create a new method. Note that if
METHOD LIST
...EMPTY 1...
...EMPTY 2...
...EMPTY 3...
OPEN DELETE NEW
there are already nine methods defined in the
list, an existing method will need to be deleted
before a new method can be added to the list.
To delete a method use the up and down
arrow keys to select a method (identified by *)
and then press the DELETE soft key. Press
the OPEN soft key to open the selected,
stored method and display the method.
4. Name the new run Press the up arrow key to select the method
METHOD: UNTITLED
S1 (EMPTY)
*
name field. Note that the soft keys have now
changed to CANCEL and OK. Use the CE
button to delete the default name and the
keypad to enter a method name. Refer to
METHODS
Section 2 for help using the keypad. Press the
CANCEL soft key to abort naming the method
or the OK soft key to accept the new name.
EDIT NAME: NAME_
S1 (EMPTY)
Note that OK accepts the name but is not
stored into memory until the method is saved.
CANCEL OK
5. Select a step to edit Press the EDIT key to open the selected
empty step (identified by *).
METHOD: NAME
S1 (EMPTY)
*
METHODS DELETE SAVE
6. Set the constant Use the soft keys to select the constant
parameter for the parameter and the keypad to enter its value. If
current step the method is complete go to Step 10,
S1/1 EDIT CONSTANT
0 V
TIME: UNTIMED
CONSTV
CONSTA CONSTW
2500
500
mA
W
otherwise, press the EDIT key. Note that the
maximum default values for the non-constant
parameters are listed on the right side of the
screen and the time mode at the bottom.
ConstV: Used to run with constant voltage.
ConstA: Used to run with constant current.
ConstW: Used to run with constant power.
9
Step Procedure Description
TIME: UNTIMED
S1/1 EDIT TIME
UNTIMED
2500
500mAW
HRS
100 V
VHOURS
7. Enter non-constant Use the soft keys to select a non-constant
parameter limits for parameter and the keypad to enter its value.
the current step If the method is complete and will be run
un-timed go to Step 10, otherwise, press the
S1/1 EDIT LIMITS
100 V
TIME: UNTIMED
CONSTV
LIMITA
2500
500
LIMITW
mA
W
EDIT key. Note: to change the constant value,
press its soft key and enter a new value.
ConstV: Used to change the voltage
constant value.
LimitA: Used to change the maximum
default value for current limit.
LimitW: Used to change the maximum
default value for power limit.
8. Select the time mode Use the soft keys to select a time mode and
the keypad to enter the run time. Press EDIT.
HRS: Run time is in units of hours.
VHOURS: Run time is in units of volt-hours.
UNTIMED: Run is continuous. Note that steps
programmed after an un-timed
step are ignored.
9. Add a new step or save To program additional steps press the down
the method arrow key to select the empty step and repeat
METHOD: NAME
S1 100V 00:30 HRS
*
S2 (EMPTY)
steps 5–8.
If the method will not be saved, go directly to
Step 10, otherwise press the SAVE soft key
and then go to Step 10. Press the DELETE
METHODS DELETE SAVE
soft key to delete the selected step. Press the
METHODS soft key to return to the Method
List screen.
10. Start the run Press to start a run.
11. Monitor the run The run screen is used to monitor run
parameters or to pause a run. The current
RUN: S1/2
535mA
100 V
00:00/00:30 00:00 HRS
PAUSE
W
step versus total steps programmed, voltage,
current and power values, elapsed time versus
the programmed time, and total elapsed time
of the method are displayed on the run screen.
Press the PAUSE soft key to edit the run
parameters (see Section 3.3.3). Press to
terminate the run. Note that any edits
made during the run will not be saved unless
SAVE is pressed at the end of the run.
12. View the run The "Run Completed" or "Run Terminated"
information screen is displayed at the end of a run. The
RUN COMPLETED AT:
05/27/03 12:34:01
200V 106mA 21W
Time: 00:01 Vh: 00003
RERUN EXIT
date and time, the final running parameter
values, the total elapsed time in hours and
volt-hours are displayed on the screen. Press
the RERUN soft key to repeat the run or the
EXIT soft key to display the Method Step List.
After the run is finished, the run data may be
transmitted to a PDA or PC (see Section 4.2.2).
10
3.3.2 Edit (or View) and Run a Saved Method
This section describes how to edit, view and run a saved method.
Step Procedure Description
1. Set up the power supply Refer to sections 3.1 for instrument setup.
2. Select the programming On the Home screen, press the METHODS
mode soft key to view the method list.
SELECT MODE
MANUAL METHODS
3. Open an existing method Use the up and down arrow keys to select a
METHOD LIST
IEF
*
NATIVE
SDS-PAGE
OPEN DELETE NEW
method to edit (identified by *) and then press
OPEN to open the Method screen. Press
to run the opened method or go to Step 4
to view and edit the method.
4. Select a step to edit Press the up and down arrow keys to select a
METHOD: SDS-PAGE
S1 100V 00:30 HRS
*
S2 300V 00:30 HRS
S3 (EMPTY)
METHODS DELETE
step. An asterisk (*) indicates the selected
step. Press EDIT to view or edit. Note: to edit
the method name, use the up arrow key to
move to the method name and activate the
name field. Use the keypad to change the
name.
5. Set the constant Use the soft keys to select the constant
parameter for the parameter and the keypad to enter its value. If
current step the method is complete skip to Step 9,
otherwise, press the EDIT key. Note that the
S1/2 EDIT CONSTANT
100 V
TIME: 00:30 HRS
CONSTV
CONSTA CONSTW
2500
500
mA
W
non-constant parameter values are listed on
the right side of the screen and the time mode
at the bottom.
ConstV: Used to run with constant voltage.
ConstA: Used to run with constant current.
ConstW: Used to run with constant power.
11
Step Procedure Description
6. Set the non-constant Use the soft keys to select a non-constant
parameter limits for parameter and the keypad to enter its value.
the current step If the method is complete skip to Step 9,
S1/2 EDIT LIMITS
100 V
TIME: 00:30 HRS
CONSTV
LIMITA
2500
500
LIMITW
mA
W
otherwise, press the EDIT key. Note: to
change the constant value, press its soft key
and enter a new value.
ConstV: Used to change the voltage
constant value.
LimitA: Used to change the current limit
value.
LimitW: Used to change the power limit
value.
7. Select the time mode Use the soft keys to select a time mode and
the keypad to enter the run time. Press the
S1/2 EDIT TIME
100 V
TIME: 00:30 HRS
VHOURS
HRS
2500
500mAW
UNTIMED
EDIT key.
HRS: Run time is in units of hours.
VHOURS: Run time is in units of volt-hours.
UNTIMED: Run is continuous. Note that steps
programmed after an un-timed
step are ignored.
8. Add a new step or If more steps are to be added to the method,
save the method use the down arrow key to move to the empty
METHOD: SDS-PAGE
S1 100V 00:30 HRS
*
S2 300V 00:30 HRS
S3 (EMPTY)
METHODS DELETE SAVE
step, press the EDIT key and repeat steps 5–8
to add a new step. If the method will not be
saved, go to Step 9, otherwise press the
SAVE soft key and then go to Step 9.
9. Start the run Press to start a run.
10. Monitor the run The run screen is used to monitor run
RUN: S1/2
535mA
100 V
00:00/00:30 00:00 HRS
W
PAUSE
parameters or to pause a run. The current
step versus total steps programmed, voltage,
current and power values, elapsed time versus
the programmed time, and total elapsed time
of the method are displayed on the run screen.
Press the PAUSE soft key to edit the run
parameters (see Section 3.3.3). Press to
terminate the run. Note that any edits
made during the run will not be saved unless
SAVE is pressed at the end of the run.
12
Step Procedure Description
11. View the run The "Run Completed" or "Run Terminated"
information screen is displayed at the end of a run. The
RUN COMPLETED AT:
05/27/03 12:34:01
200V 106mA 21W
Time: 00:01 Vh: 00003
RERUN EXIT
date and time, the final running parameter
values, the total elapsed time in hours and
volt-hours are displayed on the screen. Press
the RERUN soft key to repeat the run or the
EXIT soft key to display the Method Step List.
After the run is finished, the run data may be
transmitted to a PDA or PC (see Section 4.2.2).
3.3.3 Edit a Paused Run
This section describes how to edit a paused run.
Step Procedure Description
1. Pause the run Press the PAUSE soft key or to pause a
RUN: S1/2
535mA
100 V
00:00/00:30 00:00 HRS
W
PAUSE
run that is in progress.
2. Edit the method The pause window displays the current run
RUN PAUSED AT:
05/27/03 12:29:00
100V 53mA 5W
Time: 00:00 Vh: 00000
CONTINUEEDIT
parameters as well as the date and time that
the run was paused.
Press the EDIT soft key or the EDIT key to
modify the method. The CONTINUE soft key
or button can be used to resume the run.
3. Select a method step Press the up and down arrow keys to select
METHOD: SDS-PAGE
S1 100V 00:30 HRS
*
S2 300V 00:30 HRS
S3 (EMPTY)
CONTINUE
the step to edit or add. An asterisk (*) indicates
the selected step.
Press the EDIT key to modify the selected
step.
Press the CONTINUE soft key or to
resume the run when finished
editing the method.
4. Set the constant Use the soft keys to select the constant
parameter for the parameter and the keypad to enter its value.
current step Press the EDIT key when finished. The
S1/2 EDIT CONSTANT
100 V
TIME: 00:30 HRS
CONSTV
CONSTA CONSTW
2500
500
mA
W
key can be pressed at any time to
resume the run. Note that the non-constant
parameter
values are listed on the right side of the screen
and the time mode at the bottom.
ConstV: Used to run with constant voltage.
ConstA: Used to run with constant current.
ConstW: Used to run with constant power.
13
Step Procedure Description
5. Set the non-constant Use the soft keys to select a non-constant
parameter limits for parameter and the keypad to enter its value.
the current step Press the EDIT key when finished. The
key can be pressed at any time to
S1/2 EDIT LIMITS
100 V
TIME: 00:30 HRS
CONSTV
LIMITA
2500
500
LIMITW
mA
W
resume the run. Note: to change the constant
value, press its soft key and enter a new
value.
ConstV: Used to change the voltage
constant value.
LimitA: Used to change the current limit
value.
LimitW: Used to change the power limit
value.
6. Select the time mode Use the soft keys to select a time mode and
S1/2 EDIT TIME
100 V
TIME: 00:30 HRS
2500
500mAW
the keypad to enter the run time. Press the
EDIT key when finished and go to Step 3. The
key can be pressed at any time to resume
the run. Note that the time mode
cannot be changed for the step in progress.
HRS: Run time is in units of hours.
VHOURS: Run time is in units of volt-hours.
UNTIMED: Run is continuous. Note that steps
programmed after an un-timed
step are ignored.
7. Exit the run completed After the run is finished the "Run Terminated"
screen or "Run Completed" screen is displayed. At
RUN COMPLETED AT:
05/27/03 12:34:01
200V 106mA 21W
Time: 00:01 Vh: 00003
RERUN EXIT
this point the run data may be transmitted to a
PDA or PC, if desired (see Section 4.2.2).
Press the RERUN soft key to repeat the run or
the EXIT soft key to return to the Method Step
List screen.
8. Save the changes From the Method Step List screen, press the
METHOD: SDS-PAGE
S1 100V 00:30 HRS
*
S2 300V 00:30 HRS
S3 (EMPTY)
METHODS DELETE SAVE
SAVE soft key to save the changes made
during the run. Note that pressing SAVE will
overwrite the original method.
14
Section 4
Operation with a Personal Computer and PDA
The PowerPac Universal is capable of transmitting methods to and from a PDA, and
transmitting run data to a personal computer (PC) or PDA through the IR port located on
the front of the power supply.
4.1 Operation with a Personal Computer
Communication between a PowerPac Universal power supply and a PC requires
that the PowerPac data transfer software (Catalog Number 164-5067, includes
PowerPac remote software also) be installed on a compatible PC. The PowerPac
data transfer software for the PC is compatible with computers that run the
Windows 2000™ or Windows XP™ operating system and that include an IrDA
port. The PowerPac data transfer software is used to transmit run data from the
PowerPac Universal or PDA, to a PC. PowerPac data transfer software
instructions are contained within the help file of the software itself and will not be
discussed here.
4.2 Operation with a PDA
Communication between a PowerPac Universal power supply and a PDA requires
that the PowerPac remote software (Catalog Number 164-5067, includes PC
software also) be installed on a compatible PDA. The PowerPac remote
software is compatible with any PDA that runs Palm OS version 4.0 or above. With
the PowerPac remote software the PDA can perform the following functions:
• Transmit methods to and from the PowerPac Universal.
• Receive data from the PowerPac Universal
• Transmit data to a PC
• Create and store up to 20 methods
• Store up to 20 data files
• Provide unique data file identification
Instructions for the PowerPac remote software for PDA operation are given in
the following two sections.
4.2.1 Create, Edit and Transmit Methods
Step Procedure Description
1. Start the software Start the PowerPac Remote software and
press Select/Edit Methods.
Bio-Rad Laboratories
PowerPac Remote Control
Select/Edit Methods...
Run Data
15
Step Procedure Description
Existing Methods
DoneBeam Current Method In
Edit
Beam
Sample
New Method Purge Methods
Method: SDS-PAGE
Method Steps
Rename
Save
Delete
Beam
Methods
Delete Step
S 1 100V 2500mA 500W UNTIMED
S 2 ---Blank Step---
2. Open a method There are three ways to open a method:
a. Press New Method to create and open a
new method.
b. Select a method from the drop-down
method list and press Edit. Note that the
Edit and Beam buttons appear only if a
method from the method list has been
selected.
c. Press Beam Current Method In to
retrieve a method from the PowerPac
Universal and store it in the method list.
Note: the method in the PowerPac
Universal has to be opened before
transmission.
Other Buttons
Purge Methods: Used to delete ALL
methods stored on the PDA
Beam: Transmits the currently selected
method to the PowerPac Universal
Done: Returns to the start-up screen.
3. Edit the method Press Select/Edit Methods..., select the
method from the drop-down method list and
press Edit.
Select a step to edit from the Method Steps
drop-down menu.
16
Step Procedure Description
Method: SDS-PAGE
Step # 1
Const Mode
Rename
Volts: 100
mAmps: 2500
Watts: 500
Vhours: 00000
Untimed
Save
Delete
Beam
Methods
Delete Step
V
A
Timing
T
VhW
Bio-Rad Laboratories
PowerPac Remote Control
Select/Edit Methods...
Run Data
4. Edit a step a. Set the Constant mode: voltage (V),
current (A) or power (W).
b. Enter the constant and non-constant
values in the Volts, mAmps and Watts
fields.
c. Select whether the run will be timed
(Untimed box is not checked) or
un-timed (the Untimed box is checked).
d. Change the method name as needed.
Check the Rename box to rename the
method or leave it un-checked to save
the edited method with the new name.
e. Select another step to edit from the
"Step" drop-down list and edit the step.
f. Press Save to save the method.
Other Buttons
Delete: Deletes the entire method.
Delete Step: Deletes the current step.
Methods: Returns to Method List screen.
5. Transmit the method Press Beam to transmit the method to the
PowerPac Universal. Note that if nine
methods already exist on the PowerPac
Universal that the new method will be
transmitted to flash memory so it can be run,
but it will not be saved to the method list.
4.2.2 Receive and Transmit Data
Step Procedure Description
1. Start the software Start the PowerPac Remote software and
press Run Data
17
Step Procedure Description
Run Data
Collected Run Data
Done
Purge Data
Get Data From PowerPac
Collected Run Data
DoneBeam Delete
Get Data From PowerPac
SN 00002 15:56:05 27-May-03
This data file has not been
beamed to the PC
Purge Data
Extract Method
2. Receive data from the Press Get Data From PowerPac to request
PowerPac Universal and receive the data file from the PowerPac’s
most recent run.
Other Buttons
Purge Data: Used to delete ALL data stored
on the PDA
Done: Returns to the start-up screen.
3. Transmit data to a PC Select a data file from the drop-down Run
Data list and then press Beam to transmit the
data to a PC. The warning "This data file has
not been beamed to the PC" is displayed for
all data files that have not been transmitted to
a PC. Note that the Extract Method, Beam
and Delete buttons appear only if a run in the
Run Data list has been selected.
Other Buttons
Purge Data: Used to delete ALL data stored
on the PDA
Extract Method: Displays the method that
was used to create the selected run data.
Beam: Transmits data to a PC
Delete: Deletes the currently selected data
file.
Done: Returns to the start-up screen.
18
Section 5
Maintenance and Troubleshooting
5.1 Maintenance
The PowerPac Universal requires little maintenance to assure reliable
operation. To clean the case, first unplug the power supply. Use a damp cloth to
wipe down the outer case.
5.2 Troubleshooting
5.2.1 Basic Troubleshooting
Problem Cause Solution
No display/lights/fan 1. No AC power. 1. Check if the PowerPac
2. Blown fuse Universal is unplugged,
2. Replace the fuse. See
Repeated blown fuses Hardware failure Contact Bio-Rad Technical
Resources.
Leads from the cell are Output terminals for the Use the PowerPac Adaptor
not long enough to fit PowerPac Universal are (Catalog number 165-5061),
the output jacks recessed 16 mm to which accommodates most
meet safety regulations. standard 4 mm banana
Some leads are not plugs, to make secure
long enough to make electrical connection.
electrical connection. Note: use of this PowerPac
adaptor voids EN61010
safety provisions.
Clock loses settings Onboard battery Contact Bio-Rad Technical
on Power Off depleted Resources
or if there is a problem
with th AC power source,
or if the power switch is
in the off position.
Section 5.3 for details.
5.2.2 Power Failure Detection
The Power Failure detection mode (PFd) is used to determine the PowerPac
Universal response to power failures and is set as described in Section 3.1. There
are three Power Failure detection modes:
OFF: Power Failure detection is disabled. A run will terminate if a power
failure occurs and Error code 07 will be displayed (see Section 5.2.4).
ON NEXT RUN ONLY: Power Failure detection is enabled for a single run and
is then disabled for all subsequent runs. "PFd" is displayed in the upper right
corner of the run screen for the run in which it is active.
ON: Power Failure detection is enabled and "PFd" is displayed in the upper
right corner of the run screen. A run will resume when power is restored after
single or multiple power failures(s).
19
If a power failure occurs during a run the following screens will be displayed.
!
!
1. Displayed if PFd = ON
RUN COMPLETED AT:
05/27/03 12:35:56
200V 105mA 21W
Time: 00:01 Vh: 00002
RERUN EXITMORE
MORE is displayed on the Run Completed
screen when a run ends that has had a
power failure.
Press the MORE soft key to display details
about the most recent power outage and
when power was restored.
Press the RERUN soft key to restart the run
AC POWER INTERRUPTED
AT: 05/27/03 12:35:00
RESTARTED RUN
AT: 05/27/03 12:35:07
RERUN EXITBACK
2. Displayed if PFd = OFF
ERROR STOP: CODE 07
RUN POWER FAILURE
RUN TERMINATED.
RESET WHEN READY.
RESET
Press the BACK soft key to return to the Run
Completed screen.
Press the EXIT soft key to go to the Method
List screen
This error screen is displayed if a run has
terminated due to a power failure.
Press RESET to return to the Home screen
5.2.3 Rapid Resistance Change Detection/No Load Detection
The Rapid Resistance Change detection/No Load detection (RRCd-NLDd) is used
to determine the instruments response due to a large change in resistance or the
absence of a load. See Section 3.1 for a description of how to set this parameter.
RRCd-NLDd = ON: Detection of sudden load changes or the absence of a
load is enabled. Load changes greater than 20% or 15 mA (whichever is
greater), or the absence of a load (current is less than 2 mA), will cause an
alarm to sound, and the run to pause (See Section 5.2.4, ERROR STOP 01
and 09).
RRCd-NLDd = OFF: Detection of sudden load changes or the absence of a
load is disabled. When running in this mode, the symbol is displayed on
the Run screen as a reminder that this safety
feature has been turned off.
Note: This mode is used to complete electrophoresis application such as the
D-Code that require that Rapid Resistance Change detection be turned off (due to
possible fluctuations in current during a normal run), or applications such as
isoelectric focusing that require less than 2 mA current.
20
5.2.4 Error Messages
Error Message Cause Solution
ERROR STOP CODE 01 1. Electrophoresis cell 1. Make sure all electrical
NOLOAD DETECTED not connected to the connections make good
CORRECT AND CONTINUE power supply or contact and the cables
RUN PAUSED
CONTINUE CANCEL in good shape.
ERROR STOP: CODE 02 1. Accidental shorting 1. Make sure all electrical
OVER CURRENT of output leads. connections make good
CORRECT AND CONTINUE contact and the cables
RUN PAUSED
CONTINUE CANCEL good shape.
ERROR STOP: CODE 03 1. Control circuitry 1. Press the CONTINUE soft
OVER VOLTAGE problem key to continue the run.
CORRECT AND CONTINUE If the problem continues,
RUN PAUSED
CONTINUE CANCEL run. If the problem persists,
ERROR STOP: CODE 07 AC Power was interrupted To continue a run after a
RUN POWER FAILURE during the run and the power failure, activate the
RUN TERMINATED run was terminated Power Failure Detection mode
RESET WHEN READY
RESET failure detection is OFF Section 3.1).
ERROR STOP: CODE 08 Control circuitry 1. Press the RESET soft key to
REGULATION ERROR problems. clear the screen, then
RUN TERMINATED restart the run.
RESET WHEN READY
RESET cycle power and restart the
buffer levels too low. and wire electrodes are
Verify buffer levels are
appropriate.
2. The current load is 2. Verify the electrophoresis
less than 2 mA. application power
requirements match
PowerPac Universal output
range. Note: The No Load
detection can be deactivated. (See Sections 3.1
and 5.2.3)
and wire electrodes are in
2. Shorting due to 2. Verify buffer levels are
wrong connections. appropriate.
cycle power and restart the
contact Bio-Rad Technical
Support.
because the Power in the Setup window (See
2. If the problem continues,
run. If the problem persists,
contact Bio-Rad Technical
support.
21
Error Message Cause Solution
ERROR STOP: CODE 09 1. Loose output 1. Verify all electrical
RAPID CHANGE IN R connections leading connections.
CORRECT AND CONTINUE to intermittent
RUN PAUSED
CONTINUE CANCEL loads.
ERROR STOP: CODE 11 Insulation failure in the 1. Check electrical connections,
GROUND LEAK electrical connections electrophoresis cell, and
CORRECT AND CONTINUE made outside the power chiller system for leaks.
RUN PAUSED
CONTINUE CANCEL current flow that may cell rests on an insulated
ERROR STOP: CODE 12 Improper load, or 1. Press Reset and restart the
REGULATION ERROR connection to a run.
RUN TERMINATED voltage source 2. If the problem continues,
RESET WHEN READY
RESET run. If the problem persists,
ERROR STOP: CODE 13 Internal circuitry Cycle power and restart run.
PERM MEM ERROR problems If problem persists, contact
connection to the
2. Cells added or 2. Pause power supply prior
removed during to adding or removing
the run. electrophoresis cells
3. Change in buffer 3. Verify buffer levels are
levels appropriate.
Note: certain applications
exhibit intrinsic fluctuations in
resistance (e.g. Dcode). If this is
the case, the change in
resistance detection feature can
be disabled to allow
uninterrupted completion of the
run. (See Sections 3.1 and
5.2.3).
supply has caused a Verify that the electrophoresis
create an unsafe and dry surface.
condition. 2. Check Power source
connections. Additional
capacitance to ground from
external EMC filters or a UPS
(Uninterruptible Power
Supply) can cause
excessive ground currents.
cycle power and restart the
contact Bio-Rad Technical
support.
Bio-Rad Technical support.
22
5.3 Replacing a Fuse
If the power supply is plugged into a working outlet, with the power switch in the
ON position and there is no display, lights or fan operation, a fuse might need to be
replaced.
1. Disconnect the power cord from the electrical outlet.
2. Use a fingernail or a flat blade screwdriver to gently press the tabs on the side
of the fuse holder toward each other. This will release the fuse holder and the
fuses. See Figure 7. Inspect the fuses visually to determine if one or both of the
fuses are blown.
3. Remove the blown fuse from the fuse holder. Replace it with a 6.3A, 250VAC
5mm X 20mm fuse (Bio-Rad part number 900-7288).
4. Re-insert the fuse holder into its position. Press the fuse holder gently until it
snaps into place on both sides.
The unit is now ready for use.
FUSE
DRAWER
NOTCHES
Fig. 7. Rear view Showing Fuse Drawer with Notches.
Note: Repeated blowing of the fuse indicates a hardware failure. Contact Bio-Rad
Technical Support.
5.4 Expediting Technical Support
Make sure the following information is readily available before contacting Bio-Rad.
Product model no: Located in the sticker on the bottom of the unit
(example: PowerPac Universal Power Supply).
Serial number: Located in the sticker on the bottom of the unit
Software version: The PowerPac Universal displays the software version
momentarily after switching the power ON (example: Firmware 1.20).
State clearly the error code, error message or anomaly, and the conditions that
originated the problem, including run parameters (V, A, and W) as well as the
electrophoresis cell and buffer system.
23
Appendix A
Specifications
Output specifications: 500 V, 2500 mA, 500 W
Output range (programmable): 10–500 V, fully adjustable in 1 V increments
Type of output: Constant voltage, current, or power with
Output terminals Four pair of recessed banana jacks in
Timing mode Continuous, timed to 99:59Hrs, or
Pause/resume function: Yes
Display: 128 x 64 Monochrome Backlit LCD
Operating Conditions: 0–40°C; 0–95% humidity in the absence of
Safety compliance: EN61010
EMI: Conforms to CE Standards for emissions and
Safety features: No load detection; sudden load change
Input protection: Fuse on hot and neutral
Input power(nomial) 100–120/220–240 VAC, 50/60 Hz
Dimensions: 27.5cm X 9.8cm X 34.5 cm
Weight: 2.5 kg
10–2500 mA, fully adjustable in 1 mA
increments
1–500 W, fully adjustable in 1 W increments
automatic crossover
parallel
99999 volt-hours.
condensation
immunity class A.
detection; ground leak detection;
overload/short circuit detection; overvoltage
protection; overheating protection
auto-switching
24
Appendix B
Warranty and Ordering Information
Warranty
The PowerPac Universal power supply is covered by a standard Bio-Rad
Laboratories warranty. Contact your local Bio-Rad representative for details of the
warranty. If any defects should occur during this warranty period, Bio-Rad
Laboratories will replace the defective parts without charge. However, the following
defects are specifically excluded:
1. Defects caused by improper operation.
2. Repair or modification done by anyone other than Bio-Rad Laboratories or their
authorized agent.
3. Use with cables or connectors not specified by Bio-Rad Laboratories for this
power supply.
4. Deliberate or accidental misuse.
5. Damage caused by disaster.
For inquiry or request for repair service, contact your local Bio-Rad office.
Warranty Information
Model:
Serial Number:
Date of Delivery:
Warranty Period:
Ordering Information
Catalog
Number Description
164-5070 PowerPac Universal power supply, 100–120/220–240V
164-5067 PowerPac Universal power supply data transfer software for a PC
and PDA
164-5069 IQ/OQ Protocol and Test Box
165-5061 PowerPac Adaptor, qty 1
165-5066 PowerPac Adaptor, qty 2
900-7288 Replacement Fuse, 6.3 A, 250 VAc, 5 x 20 mm, Type T
25
Life Science
Group
Sig 1212
4006223 Rev D US/EG
Bio-Rad
Laboratories, Inc.
Web site www.bio-rad.com USA 800 424 6723 Australia 61 2 9914 2800 Austria 01 877 89 01 Belgium 09 385 55 11 Brazil 55 11 504 4 5699
Canada 905 364 343 5 China 86 21 6169 8500 Czech Republic 420 241 430 532 Denmark 44 52 10 00 Finland 09 804 22 00
France 01 47 95 69 65 Germany 089 31 884 0 Greece 30 210 9532 220 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91 124 4029300
Israel 03 963 6050 Italy 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Nethe rlands 0318 540666
New Zealand 64 9 415 228 0 Norway 23 38 41 30 Poland 4 8 22 331 99 99 Portugal 351 21 472 7700 Russia 7 495 721 14 04
Singapore 65 6415 318 8 South Africa 27 861 246 723 Spain 34 91 590 5200 Sweden 08 555 12700 Switzerland 026 674 55 05
Tai wa n 886 2 2578 7189 Thailand 800 88 2 2 88 Unite d Kingdo m 020 8328 200 0
Loading...