Bio-rad 170-3738, 170-7530, 170-3745, 170-7532 User Manual

Mini-Transilluminator

Instruction Manual

Catalog Numbers

170.3738
through

170-3745

and

170-7530

through

170-7532

Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 * Phone (510) 74 l-7000

Important

Read these instructions before operating your Mini-Transilluminator to familiarize yourself with

its operation.

Warranty

The Mini-Transilluminator is warranted against defects in materials and workmanship for 1 year.
If any defects occur in the instrument during this warranty period, Bio-Rad Laboratories will repair
or replace the defective parts at its discretion without charge. The following defects, however, are
specifically excluded:

l

Defects caused by improper operation.

l

Repair or modification done by anyone other than Bio-Rad Laboratories or an authorized
agent.

l

Use of spare parts supplied by anyone other than Bio-Rad Laboratories

l

Damaged caused by accident or misuse.

l

Damaged caused by disaster.

l

Corrosion caused by improper solvent or sample.

This warranty does not apply to parts listed below:

l

Fuses

l

Replacement bulbs

For any inquiry or request for repair service, contact Bio-Rad Laboratories. Inform Bio-Rad of

the model and serial number of your instrument.

Table of Contents

Page

Section 1
Section 2

Section 3
Section 4
Section 5
Section 6
Section 7
Section 8
Section 9

Introduction
Safety
Specification..
Ultraviolet
Transilluminator Operation
Maintenance,
Changing
Cleaning

Systems,

..................................................................................................

Information..

Spectral Outputs.. ....................................................................

the W Lamps.. ..........................................................................

the Transilluminator ..................................................................

Accessories, and

....................................................................................

................................................................................................

Repair, and

Technical Assistance..

Replacement Parts.. .......................................

1

.l

.

.2

.......................................................................

....................................

2
.3
.3
.3
.4

ii

Section 1

Introduction

Bio-Rad’s Mini-Transilluminator provides a uniform and intense source of ultraviolet

light (radiation) in a compact package. Its special design emits a high intensity excitation W

wavelength for back-illumination of transparent fluorescent materials.

The 302 run W back-illumination emitted by the Mini-Transilluminator provides a high-

ly sensitive method to detect double-strand nucleic acids that have been labeled with fluo-

rescent dyes such as ethidium bromide or acridine orange. Single strand nucleic acids may be
detected, but with lower sensitivity. The 302 nm wavelength (see Note) provides an excita­tion wavelength more sensitive for nucleic acid visualization than other 365 nm models.

NOTE: Though Bio-Rad refers to the mid-range W wavelength as 302 nm, others refer
to this region as 300 nm or 3 12 nm. The spectral output of all these regions is the same.

Section 2
Safety Information

Caution

The ultraviolet Mini-Transilluminator is a powerful source of W radiation that will cause
damage to unprotected eyes and skin. Before operating the unit, be sure all personnel in the
area are properly protected.

It is preferable that the Mini-Transilluminator be installed and operated in a darkroom

where access and exposure is limited while the unit is in operation.

Each Mini-Transilluminator is shipped with a UV blocking protective cover (except

17-7530, 170-753 1, and 170-7532). Even though this cover blocks the ultraviolet radiation

emitted by the unit, UV protective eye wear should be worn as well.

Section 3
Product Specifications

Physical Dimensions

Width
Depth

Height

.Part

Number

170-3737 302 nm
170-3738

170-3745

13.25

9.50

4.75

33.7

24.1
i2.1 cm

Wavelength VoltlHzlAmp

302 nm
302 nm

cm
cm

Nominal

Number
of Lamps Intensity

120/60/0.72 20x20 cm
220/240/50/0.62 20 x 20 cm 6X8W
1 O/50-60/0.85

2Ox20cm 6X8W

6X8W

Anal/Prep
Anal/Prep
Anal/Prep

Section 4
Ultraviolet Spectral Output

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200 300 400 500

Wavelength In Nanometers

Section 5
Operation

302 nm

1. Place the Mini-Transilluminator on a level work surface. Be sure that an air space exists
between the bottom of the unit and the work surface. This space allows proper air circu­lation through the unit.

2. Plug the female end of the power cord into the Mini-Transilluminator. For 2201240 volt
models, insure that the proper configuration of male connector or plug has been proper­ly connected to the power cord.

3. Plug the male end of the power cord into a properly grounded electrical outlet. The prop
er voltage of the Mini-Transilluminator is found on the product information label.

4. The Mini-Transilluminator comes equipped with a UV blocking protective cover unless
associated with Bio-Rad’s CCD UV Gel Documentation System with hood. If this cover
is not in place or has been removed, do not operate the unit without replacing the cover.
If the cover is missing, a W blocking protective face shield must be worn to avoid injury.
UV blocking eye wear be should worn even with the cover in place to avoid accidental

W

exposure.

5. Place the gel/sample on the filter area. Wear gloves to avoid contact with the gel and
staining agent.

6. Press the on/off (YO) switch to on (I).

7. The l/O switch should glow and the UV lamps within the unit should be glowing beneath
the filter after a momentary flickering during the start period.

8. The Mini-Transilluminator is uniquely designed with an intensity control device offering
both analytical and preparative mode settings. In the analytical mode, the Mini­Transilluminator provides nanogram level sensitivity. In the preparative mode, it is pos­sible to spend 10 minutes or longer cutting out a band with minimal photo nicking of the
DNA fragment.

9. After viewing/photographing the sample, turn the unit off (0).

10. Clean the unit surface with a damp soft cloth or sponge. Never use abrasive cleaners, as
this will damage the UV filter surface.

Section 6
Maintenance, Repair, and Technical Assistance

Bio-Rad offers technical support for all of its products. If you have any questions about

the product’s use, operation, or repair, call your local Bio-Rad office. In the U.S. call

l-800-424-6723 (800-4BIORADI.

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Section 7

Changing the UV Lamps ’

14 -

15

I24

Ds4

B4

OS5

55

1. Disconnect the Mini-Transilluminator from the electrical supply.

2. Remove the filter cover.

3. Carefully twist the W lamps from their sockets.

4. Fit with the proper replacement tubes. Refer to Section 9 for ordering information.

Section 8

Cleaning the Mini=l’kansilluminator

The painted surfaces and filter areas of the Mini-Transilluminator should be cleaned with
water, soap, and a sponge or cloth towel. Never use abrasive cleaners, solvent based cleaners,
or scouring pads.

Always disconnect the Mini-Transilluminator from electrical power prior to cleaning.

3

Section 9
Systems, Accessories, and Replacement Parts

Catalog
Number

170-3745
170-3737
170-3738
170-3742

170-3746
170-3747
170-3743

170-3748
170-3749
170-3739
170-3740
170-3741
170-3744
170-3643
170-3751
170-7530
170-7531
170-7532
100-I 157
100-l 161
100-l 153
100-l 154
100-l 155

qa\ *;;:;;’ ILoo+?a

100-l 159
loo-1 166

Product Description

Mini-Transilluminator, 100 VAC
Mini-Transilluminator, 120 VAC
Mini-Transilluminator, 220/240 VAC
Standard Documentation System, 120 VAC, includes

Mini-Transilluminator, 120 V, DS-34 camera, standard
electrophoresis hood, deep yellow DS-34 camera filter

Standard Documentation System, 100 VAC
Standard Documentation System, 220/240 VAC
Wide/Long Documentation System, 120 VAC, includes

Mini-Transilluminator, 120 V, DS-34 camera, wide/long
electrophoresis hood, deep yellow DS-34 camera filter

Wide/Long Documentation System, 100 VAC
Wide/Long Documentation System, 220 I240 VAC
Standard Electrophoresis Hood, 5’ x 7” (12.7 x 17.8 cm)
Wide/Long Electrophoresis Hood, 6” x 9” (15.2 x 22.9 cm)
DS-34 Camera
Deep Yellow DS-34 Camera Filter
Gel-Scoop, for transporting gels
Mini-Transilluminator Fluorescent Lamp, 302 nm
Mini-Transilluminator, 100 V without UV blocking cover
Mini-Transilluminator, 120 V
Mini-Transilluminator, 220/240 V
Mini-Transilluminator Filter Assembly, 20 x 20 midrange, 8 watt
Mini-Transilluminator Starter
Mini-Transilluminator Ballast, 120 VAC, preheat, 8 watt, 6
Mini-Transilluminator Ballast, 220/240 VAC, preheat, 8 watt, 6
Mini-Transilluminator Ballast, 100 VAC, preheat, 8 watt, 6

1 Amp Fuse for Mini-Transilluminator, 5 x 20 mm, 6
Mini-Transilluminator Protective Cover
Mini-Transilluminator Friction Hinge, 2

Fa replacement
number availabk when you call.

pras or

components

no( shorm hem. call

Bi*Rai technical service or your place of purchase. Pkase have the model

4

Life Science
Group

2OCQ Alfred N&et Drive
Hercules. California 94.547

Te@hone (510) 741-IWO

Fax: [phone]

Ptdlm3745REvA

~~lom#85A~Dr..MelviNe.Ne~Y~11747. Phone[phone]. Fax[phone]
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70. Bar 1276. S17124 Sdm l Phone 4&(0)&735 63 00 l Fax 46-(0)&735 54 66

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SIG 030794

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