1.4.4 Word message panel structure ................................................................................................. 8
1.5 Localization / position of safety labels on the chamber ...................................................................... 9
1.6 Type plate............................................................................................................................................ 9
1.7 General safety instructions on installing and operating the chambers ............................................. 11
1.8 Intended use ..................................................................................................................................... 12
5. START UP ............................................................................................................ 25
5.1 Behavior when opening the door ...................................................................................................... 25
6. FUNCTIONAL OVERVIEW OF THE T4.12 CHAMBER CONTROLLER ............. 25
6.1 Menu structure .................................................................................................................................. 26
6.1.1 General menu ......................................................................................................................... 26
6.1.2 Quick menu ............................................................................................................................. 28
6.1.3 “User” menu ............................................................................................................................ 28
6.2.1 Activating the “control off” mode or change to “fixed value” operating mode ......................... 29
6.3 Performance during and after power failure ...................................................................................... 31
6.4 Information ........................................................................................................................................ 31
7. CONFIGURATION OF OPTIONAL EQUIPMENT ................................................ 32
7.1 Setting the optional door heating ...................................................................................................... 32
7.2 Turning on / off the optional interior socket ....................................................................................... 33
7.3 Switching on or off the optional zero-voltage relay control outputs .................................................. 34
7.4 Functional test of the optional alarm output ...................................................................................... 34
7.5 Switching on or off the optional object temperature display ............................................................. 35
KT (E6.1) 11/2020 Page 2/139
8. SET-POINT ENTRY IN “FIXED VALUE” OPERATING MODE ........................... 35
14.3 Alarm status ...................................................................................................................................... 89
14.4 Confirming a “set” alarm .................................................................................................................... 90
14.5 Alarm configuration and overview ..................................................................................................... 90
14.5.1 List of active alarms ................................................................................................................ 91
14.5.2 History – list of all alarms ........................................................................................................ 91
14.5.3 Activating, deactivating, and testing the alarm buzzer ........................................................... 92
14.5.4 Activating / deactivating all alarm functions ............................................................................ 93
14.5.5 Setting the delay time after opening the door ......................................................................... 93
15. EVENT LIST ......................................................................................................... 94
16. GRAPHICAL DISPLAY OF THE MEASURED VALUES ..................................... 95
16.1 Setting the sampling rate .................................................................................................................. 95
16.2 Defining the display range................................................................................................................. 96
16.3 Selecting the parameters .................................................................................................................. 97
17. TEMPERATURE SAFETY DEVICES ................................................................... 97
26.1 For chambers located outside the USA and Canada ..................................................................... 134
26.2 For chambers located in the USA and Canada .............................................................................. 137
KT (E6.1) 11/2020 Page 5/139
Dear customer,
For the correct oper ation of the chambers, it is important t hat you r ead this op erating m anual com pletely
and carefully and observe all instructions as indicated. Failure to read, understand and follow the
instructions may result in personal injury. It can also lead to damage to the chamber and/or poor equipment
performance
1. Safety
1.1 Personnel Qualification
The chamber m ust only be installed, tested, and start ed up by personn el qualified for as sembly, startup,
and operation of the chamber. Qualified personnel are persons whose professional education, knowledge,
experience and knowledge of relevant standards allow them to assess, carry out, and identify any potential
hazards in the work as s igned to t hem. They must have been tra ine d an d i nstr uc t ed, a nd b e a uthorized, to
work on the chamber.
The chamber should only be operated b y laborator y personnel especially trained for this purpose and
familiar with all precautionary measures required for working in a laboratory. Observe the national
regulations on minimum age of laboratory personnel.
1.2 Operating manual
This operating m anual is part of the com ponents of delivery. Alwa ys keep it handy for referenc e in the
vicinity of the chamber. If selling the unit, hand over the operating manual to the purchaser.
To avoid injuries and damage observe th e safety instructions of the operating m anual. Failure to follow
instructions and safety precautions can lead to significant risks.
DANGER
Dangers due to failure to observe the ins truc tions and safety precautions.
Serious injuries and chamber damage. Risk of death.
Observe the safety instructions in this Operating Manual.
Follow the operating procedures in this Operating Manual.
Carefully read the complete operating instructions of the chamber prior to installing and
using the chamber.
Keep the operating manual for future reference
Make sure that all persons who use the chamber and its associated work equipment have
read and understood the Operating Manual.
This Operating Manu al is supplemented a nd updated as needed. Alwa ys use the mos t recent version of
the Operating Manual. When in doubt, call the BINDER Service Hotline for information on the up-todateness and validity of this Operating Manual.
1.3 Legal considerations
This operating manual is for informational purposes only. It contains information for correct and safe
installing, start-up, operation, decommissioning, cleaning and maintenance of the product. Note: the
contents and the product descr ibed are subj ect to cha nge with out not ice.
KT (E6.1) 11/2020 Page 6/139
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free use
and safety during operation and maintenance. Images are to provide basic understanding. They may
deviate from the actual version of the chamber. The actual scope of delivery can, due to optional or special
design, or due to recent technical changes, deviate from the information and illustrations in these
instructions this oper ating manual. In no event shall BINDER be held liable for any dam ages, direct or
incidental arising out of or related to the use of this manual.
This operating manual can not cover all conce ivable applications . If you would lik e additional inform ation,
or if special problem s arise that are not suff iciently addressed in th is manual, please ask your dealer or
contact us directly, e.g. by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or existing
agreement, descriptio n, or l ega l rel ati ons h ip, nor d o t h e y m odify such a relationship. Al l o bliga tio ns on the
part of BINDER derive from the respective purchase contract, which also contains the entire and exclusively
valid statement of warranty administration and the general terms and conditions, as well as the legal
regulations valid at the t ime the contract is concluded. The statements in this manual neit her au gment nor
restrict the contractual warranty provisions.
1.4 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations
following the harmonization of ISO 3864-2 and ANSI Z535.6.
1.4.1 Signal word panel
Depending on the probability of serious consequences, potential dangers are identified with a signal word,
the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.
WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury.
NOTICE
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.
1.4.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.
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Warning signs
Electrical hazard
Hot surface
Explosive atmosphere
or chemical burns
Pollution Hazard
Mandatory action signs
instructions
plug
Environment protection
Wear protective gloves
Wear safety goggles
Prohibition signs
water
1.4.3 Pictograms
Lifting hazard
Mandatory regulation
Harmful substances
Read operating
Risk of corrosion and /
Disconnect the power
Stability hazard
Biohazard
Lift with several persons
Do NOT touch
Information to be observed in order to ensure optimum function of the product.
Do NOT spray with
1.4.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
Instruction how to avoid the hazard: prohibition
Instruction how to avoid the hazard: mandatory action
Observe all other n otes and informat ion not necessarily em phasized in the sam e way, in order to a void
disruptions that could result in direct or indirect injury or property damage.
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Pictograms (Warning signs)
Service label
Nominal temp.
100 °C
0,80 kW / 3,0 A
Thermoelectric cooli ng
212 °F
200-230 V / 50 Hz
Peltier
IP protection
20
200-230 V / 60 Hz
Safety device
DIN 12880
1 N ~
Class
3.1
Art. No.
9020-0289
Project No.
Built
2020
Cooling incubator
BINDER GmbH
www.binder-world.com
KT 170
Serial No. 00000000000000
1.5 Localization / position of safety labels on the chamber
The following labels are located on the chamber:
Hot surface
•Inner glass door next to the glass door
handle
Read operating manual
• UL chambers: outer chamber door
• KT with optional interior socket: below
the interior socket
Figure 1: Position of labels on KT-UL
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER service for these replacements.
1.6 Type plate
The type plate is located on the left chamber side, on the bottom, right side.
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
E6.1
Figure 2: Type plate (example of KT 170 regular chamber)
Made in Germany
KT (E6.1) 11/2020 Page 9/139
Indications of the type plate
(example)
Information
BINDER
Manufacturer: BINDER GmbH
KT 170
Model
Cooling incubator
Device name
Serial No.
000000000000
Serial no. of the chamber
Built
2020
Year of construction
Nominal
100 °C
212 °F
IP protection
20
IP type of protection acc. to standard EN 60529
Temp. safety device
DIN 12880
Temperature safety device acc. to standard DIN 12880:2007
Class
3.1
Class of temperature safety device
Art. No.
9020-0289
Art. no. of the chamber
Project No.
---
Optional: Special application acc. to project no.
0,80 kW
Nominal power
3,0 A
Nominal current
200-230 V / 50 Hz
200-230 V / 60 Hz
1 N ~
Current type
Thermoelectric cooling Peltier
Peltier refrigerating technology
Symbol on the type plate
Information
LABORATORY EQUIPMENT
43KM
temperature
Nominal temperature
Nominal voltage range +/-10% at the indicated power
frequency
CE conformity marking
Electrical and electronic equipment manufactured / placed on
the market in the EC after 13 August 2005 and to be
disposed of in separate collection according to Directive
2012/19/EU on waste electrical and electronic equipment
(WEEE).
(KT-UL only)
GS mark of conformity of the “Deutsche Gesetzliche
Unfallversicherung e.V. (DGUV), Prüf- und
Zertifizierungsstelle Nahrungsmittel und Verpackung im
DGUV Test“ (German Social Accident Insurance (DGUV),
Testing and Certification Body for Foodstuffs and Packaging
Industry in DGUV Test). (Not valid for UL chambers)
The chamber is certified according to Customs Union
Technical Regulation (CU TR) for the Eurasian Economic
Union (Russia, Belarus, Armenia, Kazakhstan Kyrgyzstan).
The chamber is certified by Underwriters Laboratories Inc.
®
according to the following standards:
rd
• UL 61010-1, 3
• CAN/CSA-C 22.2 No. 61010-1, 3
Edition, 2012-05, Rev. 2015-07
rd
Edition, 201 2-05, Rev.
2015-07
rd
• IEC/EN 61010-1:2014, 3
Edition
KT (E6.1) 11/2020 Page 10/139
1.7 General safety instructions on installing and operating the chambers
With regard to operating the chambers and to the installation location, please observe the local and national
regulations relevant for your country (for Germany: DGUV guidelines 213-850 on safe working in
laboratories, issued by the employers’ liability insurance association).
BINDER GmbH is only respons ible for th e safet y features of th e chamber provided sk illed elec tricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating
to chamber safety are replaced in the event of failure with original spare parts.
To operate the chamber, use only original BINDER ac cessories or ac cessories f rom third-par ty suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
NOTICE
Danger of overheating due to lack of ventilation.
Damage to the chamber.
∅ Do NOT install the chamber in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Observe the prescribed minimum distances when installing the chamber (chap. 3.4)
Do not install or operate the chamber in hazar dous lo c ations .
DANGER
Danger of explosion due to combustible du sts or explosive mixtures in the vicinity
of the chamber.
Serious injury or death from burns and / or explo sion pressure.
∅ Do NOT operate the chamber in potentially explosive areas.
KEEP combustible dust or air-solvent mixtures AWAY from the chamber.
The chamber does not dispose of any measures of explosion protection.
DANGER
Danger of explosion due to introduction of flammable or explosive substances in
the chamber.
Serious injury or death from burns and / or explo sion pressure.
∅ Do NOT introduce any substance into the chamber which is combustible or explosive at
working temperature.
∅ Do NOT introduce any combustible dust or air-solvent mixture in the inner chamber.
Any solvent contai ned in th e charging m aterial m ust not be explosive or inflammabl e. I.e., irres pective of
the solvent concentrati on in the steam room, NO explos ive mixture with air must f orm. The temperature
inside the cham ber m ust lie belo w the f lash point or belo w the su blim ation point of the ch arging mater ial.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the
contained moisture constituent and its behavior with the addition of heat energy.
Familiarize yourself with any potential health risks caused by the charging material, the contained moisture
constituent or by reaction products which may arise during the temperature process. Take adequate
measures to exclude such risks prior to putting the chamber into operation.
KT (E6.1) 11/2020 Page 11/139
DANGER
Electrical hazard by water entering the chamber.
Deadly electric shock.
∅ The chamber must NOT become wet during operation, cleaning, or maintenance.
∅ Do NOT install the chamber in damp areas or in puddles.
Set up the chamber in a splash-proof manner.
The chambers were produced in accordance with VDE regulations and were routinely tested in accordance
to VDE 0411-1 (IEC 61010-1).
During and shortl y after operation, th e temperatur e of the inner s urfac es almos t equals the set-point. The
glass door, the glass door handles, and the inner chamber will become hot during operation.
CAUTION
Danger of burning by touching hot chamber parts during operation.
Burns.
∅ Do NOT touch the glass door, the glass door handles, the inner surfaces or the
charging material during oper atio n.
1.8 Intended use
Following the instructions in this operating manual and conducting regular maintenance work
(chap. 21.2) are part of the intended use.
Any use of the chambers that does not comply with the requirements specified in this Operating
Manual shall be considered improper use.
Other applications than those described in this chapter are not approved.
Use
Cooling incubators KT are suitab le for exac t conditioni ng of harmles s materials. Because of the ir precise
temperature accurac y these d evices ar e espec ially us eful for c ultivation of m icroorgan isms with a narro w
temperature optim um in a range of 4 °C / 39.2°F to 37 °C / 98.6°F. Main fields of app lication are t ests of
long-term stor age (e.g. at 4 °C / 39.2°F), ref rigerated incubat ion between 20 ° C / 68°F and 25 °C / 77°F
and incubation at 37 °C / 98.6°F (also with add iti on al int roduc t ion of heat) or with alter n ati ng temperatures
(e.g. 37 °C / 98.6°F and 4 °C / 39.2°F).
Requirements for the chamber load
Any solvent must not be explosive and flammable. A mixture of any component of the loading material with
air must NOT be explosive. The operating temperature must lie below the flash point or below the
sublimation point of the chargin g material. Any com ponent of the c harging m aterial must NO T be able to
release toxic gases.
The charging material shall not contain any corrosive ingredients that may damage the machine
components made of stain less steel, aluminum , and copper. Such ingr edients include in part icular acids
and halides. Any corrosive damage caused by such ingredients is excluded from liability by BINDER GmbH.
KT (E6.1) 11/2020 Page 12/139
The chamber does not dispose of any measures of explosion protection.
DANGER
Explosion or implosion hazard and danger of poisoning through the introduction of
unsuitable loading material.
Poisoning. Serious injury or death from burns and / or explosion pressure.
∅ Do NOT introduce any substance combustible or explosive at working temperature into
the chamber, in particular no energy sources such as batteries or lithium-ion batteries.
∅ NO explosive dust or air-solvent mixture in the inner chamber.
∅ Do NOT introduce any substance which could lead to release of toxic gases.
Contamination of the chamber by toxic, infectious or radioactive substances must be prevented
WARNING
Danger of intoxication and infection through contamination of the chamber with
toxic, infectious or radioactive substances.
Damages to health.
Protect the interior of the chamber from contamination by toxic, infectious or radioactive
substances.
Take suitable protective measures when introducing and removing toxic, infectious or
radioactive material
In case of foreseeable use of the chamber there is no risk for the user through the integration of the chamber
into systems or by special environmental or operating conditions in the sense of EN 61010-1:2010. For this,
the intended use of the chamber and all its connections must be observed.
Medical devices
The chambers are not classified as medical devices as defined by the Medical Device Directive 93/42/EEC.
Due to the special demands of the Medical Device Directive (MDD), these chambers are not
qualified for sterilization of medical devices as defined by the directive 93/42/EWG.
Personnel Requirements
Only trained personnel with knowledge of the Operating Manual can set up and install the chamber, start it
up, operate, clean, and tak e it out of op eration. Ser vic e and repairs call for f urther techn ical req uirem ents
(e.g. electrical know-how), as well as knowledge of the service manual.
Installation site requirements
The chambers are designed for setting up inside a building (indoor use).
The requirements des cribed in the Operating Manua l for installation site and ambient conditions (Chap.
3.4) must be met.
WARNING: If customer should use a BINDER chamber running in non-supervised
continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen
or samples to split such specimen or samples and store them in at least two chambers, if
this is feasible.
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1.9 Foreseeable Misuse
Other applications than those described in chap. 1.8 are not approved.
This expressly incl udes the following misuses (the list is not exhaustive), which pos e risks despite the
inherently safe construction and existing technical safety equipment:
• Non-observance of Operating Manual
• Non-observance of information and warnings on the chamber (e.g. control unit messages, safety
identifiers, warning signals)
• Installation, startup, operation, maintenance and repair by untrained, insufficiently qualified, or
unauthorized personnel
• Missed or delayed maintenance and testing
• Non-observance of traces of wear and tear
• Insertion of materials excluded or not permitted by this Operating Manual.
• Non-compliance with the admissible parameters for processing the respective material.
• Installation, testing, service or repair in the presence of solvents
• Installation of replacement parts and use of accessories and operating reso urces not specified and
authorized by the manufacturer
• Installation, startup, operation, maintenance or repair of the chamber in absence of operating
instructions
• Bypassing or c hang in g pr ot ec tive systems, operation of the c hamber without the designated pr otec t ive
systems
• Non-observance of messages regarding cleaning and disinfection of the chamber.
• Spilling water or clean ing agent on the cham ber, water penetrating into the c hamber dur ing operat ion,
cleaning or maintenance.
• Cleaning activity while the chamber is turned on.
• Operation of the chamber with a damaged housing or damaged power cord
• Continued operation of the chamber during an obvious malfunction
• Insertion of objects, particularly metallic objects, in louvers or other openings or slots on the chamber
• Human error (e.g. insufficient experience, qualification, stress, exhaustion, laziness)
To prevent these and other risks fr om incorrect operati on, the operator s hall issue operating instruc tions.
Standard operating procedures (SOPs) are recommended.
KT (E6.1) 11/2020 Page 14/139
1.10 Residual Risks
The unavoidable design features of a chamber, as well as its proper field of application, can also pose risks,
even during correct operation. These residual risks include hazards which, despite the inherently safe
design, existing technical protective equipment, safety precautions and supplementary protective
measures, cannot be ruled out.
Messages on the chamber and in the Operating Manual warn of residual risks. The consequences of these
residual risks and the measures required to prevent them are listed in the Operating Manual. Moreover, the
operator must take measures to minimize hazards from unavoidable residual risks. This includes, in
particular, issuing operating instructions.
The following list s ummarizes the hazar ds against which this Operating Manua l and the Service Manua l
warn, and specifies protective measures at the appropriate spots:
Unpacking, Transport, Installati on
• Sliding or tilting the chamber
• Setup of the chamber in unauthorized areas
• Installation of a damaged chamber
• Installation of a chamber with damaged power cord
• Inappropriate site of installation
• Missing protective conductor connection
Normal operation
• Assem bly errors
• Contact with hot surfaces on the housing
• Contact with hot surfaces in the interior and inside of doors
• Emission of non-ionizing radiation from electrical operating resources
• Contact with live parts in normal state
Cleaning and Decontamination
• Penetration of water into the chamber
• Inappropriate cleaning and decontamination agents
• Enclosure of persons in the interior
Malfunction and Damage
• Continued oper atio n of th e c ham ber during an ob vious m alfunc tion or out age of the he ating or cooling
system
• Contact with live parts during error status
• Operation of a unit with damaged power cord
Maintenance
• Maintenance work on live parts.
• Execution of maintenance work by untrained/insufficiently qualified personnel
• Electrical safety analysis during annual maintenance not performed
Trouble-shooting and Repairs
• Non-observance of warning messages in the Service Manual
• Trouble-shooting of live parts without specified safety measures
KT (E6.1) 11/2020 Page 15/139
• Absence of a plausibility check to rule out erroneous inscription of electrical components
• Performance of repair work by untrained/insufficiently qualified personnel
• Inappropriate repairs which do not meet the quality standard spec if ied b y BINDER
• Use of replacement parts other than BINDER original replacement parts
• Electrical safety analysis not performed after repairs
1.11 O perating instructions
Depending on the a pplicati on and loc ation of the chamber, the operator of the chamber mus t provide th e
relevant information for safe operation of the chamber in a set of operating instructions.
Keep these operating instructions with the chamber at all times in a place where they are
clearly visible. They must be comprehensible and written in the language of the employees.
1.12 Measures to prevent accidents
The operator of the chamber must observe the following rule: “Betreiben von Arbeitsmitteln. Betreiben von
Kälteanlagen, Wärmepumpen und Kühleinrichtungen“ (Operation of work equipment. Operation of
refrigeration systems, heat pumps and refrigeration equipment) (GUV-R 500 chap. 2.35) (for Germany).
The manufacturer took the follo wing m eas ures to prevent ign iti on and ex plos io ns :
•Indications on the type plate
See operating manual chap. 1.6.
•Operating manual
An operating manual is available for each chamber.
•Overtemperature monitoring
The chamber is equipped with a temperature display, which can be read from outside.
The chamber is equipped with an addition al saf ety contr oller ( tem perature s afet y device clas s 3.1 acc .
to DIN 12880:2007). Visual and audible (buzzer) signals indicate temperature exceeding.
•Safety, measurement, and control equipment
The safety, measuring, and control equipment is easily accessible.
•Electrostatic ch arg e
The interior parts are grounded.
•Non-ionizing radiation
Non-ionizing radiation is not intentionally produced, but released only for technical reasons by electrical
equipment (e.g. elec tr ic motors, power cables, sol eno i ds) . The machine has no permanent magnets. If
persons with active im plants (e.g. pacemakers, def ibrillators) keep a safe distance (distance of field
source to implant) of 30 cm, an influence of these implants can be excluded with high probability.
•Protection against touchable surfaces
Tested according to EN ISO 13732-1:2008.
•Floors
See operating manual chap. 3.4 for correct installation
KT (E6.1) 11/2020 Page 16/139
•Cleaning
See operating manual chap. 21.3.
•Examinations
The chamber has been inspected by the “Deutsche Gesetzliche Unfallversicherung e.V. (DGUV)
(German Social Accident Insurance (DGUV)” (German Social Accident Insurance (DGUV), Testing and
Certification Body for Food stuffs and Pack aging Indus try in DGU V Test) and bears the GS mark. (Not
valid for UL chambers)
®
UL units only: The cham ber is certified by Underwr iters Laboratories Inc.
rd
standards: UL 61010-1, 3
2012-05, Rev. 2015-07; IEC/EN 61010-1:2014, 3
Edition, 2012-05, Rev. 2015-07; C AN/CS A-C22.2 N o. 61010-1, 3rd Edition,
rd
Edition
accord-ing to the following
2. Chamber description
A high level of precision, reliability, and safety for all growth parameters ensures optimum incubation
conditions. Moreover , the KT cooling i ncubator is designed f or maximum usability – even in continuous
operation year after year. It fulfills all technical and application-specific requirements arising in
experimentation such as in the areas of biotechnology, medicine, the nutrition industry, pharmaceutical and
cosmetics industries, botany, and zoology.
Two important temperature technologies have been combined to achieve perfect temperature control. The
Peltier refrigerating system, in conjunc tion with the APT .line™ preheating chamber technology, satisf ies
the unique prerequisites for attaining highly-precise temperature control and particularly short recovery
times after opening the door.
The refrigerating s ystem is dis tingu ished b y direc t, pr ec ise, and r apid tem perature conduct ion . Due to the
Peltier cooling, s hocks are om itted which would occ ur during start and stop of c onventional refrigerat ion
systems with a compressor.
The APT.line™ preheating chamber system ensures high level of spatial and time-based temperature
precision, thanks to the direct and distributed air circulation into the interior. This is especially important for
maintaining temper atures – especially with full cham bers – and for rapid restor ation of optimum growth
conditions after ope ning the door. The inner glass door ensures that the tem perature remains constant
when observing the incubation process. The air turbine supports exact attainment and maintenance of the
desired temperature accuracy. The fan speed is digitally adjustable. The heating and refrigerating systems
are microprocess or regulated to a te nth of a degree. In addition, the chamber provides alm ost unlimited
possibilities for adaptation to individual customer requirements based upon extensive programming options
and on the week program timer and real time clock of the controller.
All chamber f unctions are easy and comf ortable to us e thanks to the ir clear arra ngement. Maj or features
are easy cleaning of all chamber parts and avoidance of undesired contamination.
The inner chamber , the pre-heating cham ber and the interior si de of the doors a re all made of stainles s
steel V2A (German material no. 1.4301, US equivalent AISI 304). The housing is RAL 7035 powder-coated.
All corners and edges are also completely coated.
The chambers come equipped with an Ethernet interfac e for computer comm unication, e.g. via the APTCOM™ 4 Multi Management Software (option, chap. 19.1). For further options, see chap. 23.5.
Temperature range with an ambient temperature of +22 °C +/- 3 °C / 71.6 °F +/- 5.4 °F:
+4 °C / 23 °F up to +100 °C / 212 °F
Fan speed range with temperature values from 4 °C up to 70 °C: 40 % up to100 %
Fan speed with temperature values above 70 °C: 100 %
KT (E6.1) 11/2020 Page 17/139
2.1 Chamber overview
Instrument panel with
microprocessor controller
T4.12 and USB interface
Door handle
Chamber door
Main power switch
2.2 Instrument panel
Figure 3: Cooling incubator KT (example: model KT 170)
5,7" controller display
Context-sensitive buttons
USB interface
Operating button
Pilot lamp: ready for operation
Figure 4: Instrument panel with microprocessor controller T4.12 and USB interface
KT (E6.1) 11/2020 Page 18/139
(7) (8)
2.3 Chamber rear
(1)
(3)
(4)
(6)
Figure 5: Chamber rear with position of options (example KT 170)
(1) DIN-socket for zero-voltage relay alarm outputs (option)
(2) (not used)
(3) DIN socket for analog output 4-20 mA (option)
(4) DIN-socket for zero-voltage relay control outputs (option)
(5) (not used)
(6) Ethernet interface for computer communication
(7) Peltier fan grid
(8) Socket for IEC connector plug with power cable
KT (E6.1) 11/2020 Page 19/139
nd 115 from the pa llet with the aid of four peopl e in the area of
3. Completeness of delivery, transportation, storage, and location of
installation
3.1 Unpacking, and checking equipment and completeness of delivery
After unpacking, p lease check the chamber and its optional acces sories, if any, based on the del ivery
receipt for completenes s and for transportation damage. Inf orm the carrier immediately if tr ansportation
damage has occurred.
The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has
no impact on the function and performance of the chamber.
Please remove any transportati on protection devices and adhes ives in/on the chamber and on the do ors
and remove the operating manuals and accessory equipment.
CAUTION
Risk of injury and damages by lifting heavy loads and by sliding or tilting of the
chamber due to improper lifting.
Injuries, damage to the chamber.
Do NOT lift or transport the chamber using the door or the door handle.
Lift chambers size 53 a
all 4 chamber feet.
Lift chambers size 170 from the pallet with the aid of six people in the area of all 4
chamber feet.
If you need to return the chamber, please use the original packing and observe the guidelines for safe lifting
and transportation (chap. 3.2).
For disposal of the transport packing, see chap. 22.1.
Note on second-hand chambers (Ex-Demo-Units):
Second-hand chambers are chambers that were use d for a short time for tests or exhib itions. They are
thoroughly tested before resale. BINDER ensures that the chamber is technically sound and will work
flawlessly.
Second-hand chambers are marked with a sticker on the chamber door. Please remove the sticker before
commissioning the chamber.
KT (E6.1) 11/2020 Page 20/139
NOTICE
3.2 Guidelines for safe lifting and transportation
After operation, please observe the guidelines for temporary decommissioning (chap. 22.2).
CAUTION
Risk of injury and damages by lifting heavy loads and by sliding or tilting of the
chamber due to improper transportation.
Injuries, damage to the chamber.
Transport the chamber in its original packaging only.
For moving or shipping, secure the chamber with transport straps.
Do NOT lift or transport the chamber using the door or the door handle.
Lift the chamber size 53 and 115 at the four lower corners with the aid of 4 people and
place it on a rolling pallet.
Lift the chamber size 170 at the four lower corners with the aid of 4 people and place it
on a rolling pallet.
Permissible ambient temperature range during transport: 10 °C / 14°F to +60 °C / 140°F.
You can order transport packing for moving or shipping purposes from BINDER service.
3.3 Storage
Intermediate storage of the chamber is possible in a closed and dr y room. Observe the guidelines for
temporary decommissioning (chap. 22.2).
• Permissible ambient temperature range during storage: -10 °C / 14°F to +60 °C / 140°F.
When after storage in a cold location you transfer the chamber to its warmer installation site, condensation
may form. Befor e s tart -up, wait at le as t o ne ho ur un ti l t he c hamber has attained a mbient temperature and
is completely dry.
3.4 Location of installation and ambient conditions
Set up the chamber on a flat, even surface, free from vibration, in a well-ventilated, dry location and align it
using a spirit level. The site of installation must be capable of supporting the chamber’s weight (see
technical data, chap. 23.4). The chambers are designed for setting up inside a building (indoor use).
Danger of overheating due to lack of ventilation.
Damage to the chamber.
∅ Do NOT install the chamber in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Observe the prescribed minimum distances when installing the chamber.
KT (E6.1) 11/2020 Page 21/139
Do not install or operate the chamber in potentially explosive areas.
DANGER
Danger of explosion due to combustible du sts or explosive mixtures in the vicinity
of the chamber.
Serious injury or death from burns and / or explo sion pressure.
∅ Do NOT operate the chamber in potentially explosive areas.
KEEP explosive dust or air-solvent mixtures AWAY from the vicinity of the chamber.
Ambient conditions
• Permissible ambient temperature range during operation: +18 °C / 64.4 °F to +25 °C / 77 °F.
At elevated ambient temperature values, fluctuations in temperature can occur.
The ambient temperature should not be substantially higher than the indicated ambient
temperature of +22 °C +/- 3 °C / 71.6 °F ± 5.4 °F to which the specified technical data
relates. Deviations from the indicated data are possible for other ambient conditions.
Lower values of the temperature range indicated in the technical data are valid at an
ambient temperature of max. 25 °C / 77 °F.
With each degree of ambient temperature > +25 °C / 77°F, the refrigeration power
decreases by 1.5 K.
When operating the chamber at temperature set-points below ambient temperature, high ambient
humidity may lead to condensation on the chamber.
• Installation height: max. 2000 m / 6.6 ft. above sea level.
Minimum distances
• When placing several chambers of the same size side by side, maintain a minimum distance of 250 mm
/ 9.84 in between each chamber.
• Wall distances (minimum distances): rear 100 mm / 3.94 in, sides 240 mm / 9.45 in.
• Spacing above the chamber of at least 100 mm / 3.94 in must also be maintained
Stacking
Two devices of the following sizes can be piled on top of each other:
• KT 53 on KT 53 or KT 115 or KT 170
• KT 115 on KT 115 or KT 170
• KT 170 on KT 170
Place rubber pads under all four feet of the upper chamber to prevent the device from slipping.
NOTICE
Danger of sliding or tilting of the upper chamber when stacking.
Damage to the chambers.
When stacking, place rubber pads under all four feet of the upper chamber.
Other requirements
To completely separat e the chamber f rom the power s upply, you m ust disconnect the power p lug. Install
the chamber in a way that the power plug is easily accessible and can be easily pulled in case of danger.
For the user there is no risk of temporary overvoltages in the sense of EN 61010-1:2010.
KT (E6.1) 11/2020 Page 22/139
With an increased amount of dust in the ambient air, clean the Peltier fan grid (7) by suction or b lowing
several times a year.
Avoid any conductive dust in the ambiance according to the chamber layout complying with pollution degree
2 (IEC 61010-1).
4. Installation of the equipme nt
4.1 Spacer for wall distance
Please fix both spacers with the supplied screws at the chamber rear. This serves to ensure the prescribed
minimum distance to the rear wall of 100 mm / 3.94in.
Figure 6: Spacer for wall distance
Figure 7: KT 170 rear with mounted spacers
KT (E6.1) 11/2020 Page 23/139
KT 53
KT 170
KT 53-UL
KT 170-UL
NOTICE
4.2 Electri cal co nn ect io n
The chambers are supplied ready for connection. They come with an IEC connector plug.
Model Power plug
KT 115
KT 115-UL
• The domes tic socket must also provide a protec tive conductor. Make s ure that the connection of the
protective conductor of the domestic installations to the chamber’s protective conductor meets the latest
technology. The protective conductors of the socket and plug must be compatible!
Shock-proof plug
NEMA 5-15P
Nominal voltage ± 10% at the
indicated power frequency
200-230 V at 50 Hz
200-230 V at 60 Hz
100-120 V at 50 Hz
100-120 V at 60 Hz
Current
type
1N~ 10 A
1N~ 10 A
Chamber
fuse
DANGER
Electrical hazard due to missing protective conductor connection.
Deadly electric shock.
Make sure that the chamber’s power plug and the power socket match and
securely connect the electrical protective conductors of the chamber and the house
installation.
• Only use original connection cables from BINDER according to the above specification.
• Prior to con nection and s tar t-up, chec k the po wer su ppl y voltag e. Com pare th e v alues to t he sp ecif ied
data located on the chamber’s type plate (left chamber side, bottom right-hand, see chap. 1.6).
Danger of incorrect power supply voltage due to improper connection.
Damage to the chamber.
Check the power supply voltage before connection and start-up.
Compare the power supply voltage with the data indicated on the type plate.
• When connect ing, please o bserve the regul ations spec ified by the local e lectricity s upply com pany as
well as the local or national electrical regulations (VDE directives for Germany).
• Observe a sufficient current protection according to the number of devices that you want to operate. We
recommend the use of a residual current circuit breaker.
• Pollution degree (acc. to IEC 61010-1): 2
• Over-voltage category (acc. to IEC 61010-1): II
See also electrical data (chap. 23.4).
To completely separate the chamber from the power supply, you must disconnect the power
plug. Install the chamber in a way that the power plug is easily accessible and can be easily
pulled in case of danger.
KT (E6.1) 11/2020 Page 24/139
5. Start up
After connecting the e lect ri c al su pply (chap. 4.2) turn on the chamber by the main power sw itch. The pilot
lamp shows the chamber is ready for operation.
Observe a delay time of about 30s between turning Off and On again. Otherwise an
initialization problem may occur.
Note that the cham ber is in stand-by mode when the main power s witch has bee n turned on and yet the
controller display is dark. Turn on the chamber by pressing any controller button.
Warming chambers may release odors in the first few days after commissioning. This is not a quality defect.
To reduce odors quick ly we recomm end heating up the chamber to its nominal tem perature for one day
and in a well-ventilated location.
WARNING: If customer should use a BINDER chamber running in non-supervised
continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen
or samples to split such specimen or samples and store them in at least two chambers, if
this is feasible.
5.1 Behavior when opening the door
When you open the door, heating and fan turn off. After a delay time of von 60 seconds (KT 53), 40 seconds
(KT 115) or 20 seconds (KT 170) they turn on again.
6. Functional overview of the T4.12 chamber controller
The T4.12 chamber controller controls the temperature (range: 4 °C up to 100 °C) and the fan speed (range:
40 % up to 100 %) inside the chamber. You can enter the desired set point values in fixed value operating
mode or in program mode in the display controller. The controller also offers a week program function and
various notifications and alarm messages with visual and audible indication, a trace file and remote alarms
via e-mail. You can enter values or program s directl y at the controller k eypad or using th e APT-COM™ 4
Multi Management Software (option, chap. 19.1) specially developed by BINDER.
Temperature values
Fan speed value
Actual values
Set-point values
Icons:
Controller operating in “fixed
value” operating mode
From the Initial view you ha ve acc es s to dif f erent menus using the menu buttons “User”, “Quick menu” , or
“Menu”. From there you can access the desired control functions. To do this, select the function by turning
the operating button and press the operating button to confirm the selection.
In any menu, you can re turn to the previous disp lay pressing t he "Close" button or to th e initial vie w with
the "Home" button.
Depending on the logged-in user or administrator, the available menu functions may vary. These
instructions present the functions which are available to the logged-in administrator.
Initial view (sample values).
Press the desired menu button.
6.1.1 General menu
The general menu provides access to all setting functions of the controller, a graphical display of the
measured values, and the possibility to read and give out data via the USB interface. In addition, supporting
functions like a settings wizard or a contact page are available.
08.03.2015 05:05:06
General menu
Turn the operating butt on to see additional menu items.
KT (E6.1) 11/2020 Page 26/139
Fixed value
..\ Menu
Measurement chart
Optional equipment
Sensor adjustment
Service contact
System information
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General menu (next page)
(“Optional equipm ent” menu item is visible onl y with
optional chamber equipment)
Close Home
Controller mode
Switching between the operating modes “control off” or “fixed value”,
chap. 6.2.1
Event list Display of status information and errors, chap. 15
Alarms Alarm settings, chap. 14.5
Setpoints Setpoint entry in “Fixed value” operating mode, chap. 8
Safety controller Setting the safety controller, chap. 17.2
Programs Time and week programs, chap. 9 and 10
Import/Export Data transfer via USB interface, chap. 13
Settings General controller settings, chap. 12
Measurement chart Graphical display of the measured values, chap. 16
Setting for optional equipment like door heating, interior socket,
Optional equipment
zero-voltage relay control outputs, alarm output, object temperature
display, chap. 7
(menu item is visible only with optional chamber equipment)
Sensor adjustment
Adjustment menu for single-point and two-point adjustments (for
Service purpose)
Service contactService information
System information Chamber information (model, name, serial no., firmware etc.)
KT (E6.1) 11/2020 Page 27/139
Fixed value
..\ Quick menu
Measurement chart
Active alarms
Temperature setpoint
Fan speed setpoint
Safety controller setpoint
Time program
Week program
Close
Home
Fixed value
..\ User
Key lock
Show event list
6.1.2 Quick menu
The Quick menu provides fast access to frequently used functions.
08.03.2015 05:05:06
“Quick menu”
Measurement chart
Active alarms
Temperature setpoint
Fan speed setpoint
Safety controller setpoint
Time program
Week program
Graphical display of the measured values, chap. 16
Alarm settings, chap. 14.5
Temperature setpoint entry in “Fixed value” operating mode, chap. 8
Fan speed setpoint entry in “Fixed value” operating mode, chap. 8
Setting the safety controller setpoint, chap. 17.2
Starting and cancelling a time program, chap. 9.1, 9.2
Starting and cancelling a week program, chap. 10.1, 10.2
6.1.3 “User” menu
The user menu includes the key lock function and provides quick access to the event list. The key lock
function serves to block the access to the controller. An overview of logon, logoff, and other events is
given in the event list.
08.03.2015 05:05:06
Key lock Configuring the key lock function, chap. 11
Show event list Displaying the event list, chap. 15
KT (E6.1) 11/2020 Page 28/139
“User” menu
Close Home
Fixed value
..\ Menu
Controller mode
Event list
Alarms
Setpoints
Safety controller
Programs
Import/Export
Settings
Fixed value
..\ Controller mode
Control off
Fixed value
6.2 Operating modes
In the “control off” mode (chap. 6.2.1), the controller is non-functional and displays only the actual values.
There is no heating or ref rigeration. The tem perature approxim ates the ambient va lue, the fan turns with
40 % speed.
You can enter the d esired s et point values in “fixed v alue” mode ( chap. 8). T he controller t hen operates
as a fixed-point control, i.e., it reaches and maintains the defined temperature set-point until the next manual
change.
The T4.12 program c ontroller also perm its running a time program (chap. 9) or a week p rogram (chap.
10). You can program temperature cycles and define also the fan sp eed for each program section. The
controller offers 52 time program places with u p to 100 sections e ach. The week progr am mode offer s 8
week program places with up to 30 shift points for each week program.
6.2.1 Activating the “control off” mode or change to “fixed value” operating mode
To select the “control off” or “fixed value” operating mode, go to Menu > Controller mode
08.03.2015 05:05:06
Close Home
Close Home
General menu.
Select “Controller Mode”
and press the operating button.
08.03.2015 05:05:06
Submenu “Controller Mode”.
Select the desired controller mode
“Control off” or “Fixed value”
and press the operating button.
KT (E6.1) 11/2020 Page 29/139
Control off
..\ Menu
Controller mode
Event list
Alarms
Setpoints
Safety controller
Programs
Import/Export
Settings
Control off
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Actual value
25.2
40.0
Menu
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General menu with controller mode “Control off”.
The controller mode “Fixed value” or “Control off” is
indicated in the display headline.
Close Home
Go back to the initial view with “Home”.
Temperature [°C]
Fan [%]
User Quick menu
The controller is non-functional, i.e., there is no heating or refrigerating. The fan turns at 40 % speed.
In the “Control off” mode, no program can be started.
Initial view in “Control off” mode
(sample picture).
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