Beckman Coulter Allegra X-15R User manual

Name: Beckman Coulter Allegra X-15R
Brand: Beckman
SKU: 7455001
Rating: 4.3 (7 reviews)

4.3 (7)

Instructions For Use

Allegra X-15R

Centrifuge

PN GXR-IM-4AC

October 2013

Beckman Coulter, Inc.

250 S. Kraemer Blvd.

Brea, CA 92821 U.S.A.

Allegra X-15R

Centrifuge

PN GXR-IM-4AC (October 2013)

All rights reserved. No part of this document may be reproduced or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without prior written permission from Beckman Coulter, Inc.

Beckman Coulter, the stylized logo, and Allegra are trademarks of Beckman Coulter, Inc. and are registered in the USPTO.

All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.

Find us on the World Wide Web at:

www.beckmancoulter.com

Made in U.S.A.

Printed in U.S.A.

Safety Notice

Read all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate instrument. Do not attempt to perform any procedure before carefully reading all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative.

Alerts for Warning, Caution, and Note

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury.

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided, may result in minor or moderate injury and/or mechanical damage.

NOTE NOTE is used to call attention to notable information that should be followed during installation, use, or servicing of this equipment.

Safety During Installation and/or Maintenance

This centrifuge weighs 128 kg (283 lb). Do not attempt to lift or move it without assistance.

Any servicing of this equipment that requires removal of any covers can expose parts that involve the risk of electric shock or personal injury. Make sure that the power switch is off and the centrifuge is disconnected from the main power source by removing the Mains (power) plug from the outlet receptacle, and refer such servicing to qualified personnel.

Do not replace any centrifuge components with parts not specified for use on this instrument.

Electrical Safety

To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect the centrifuge to earth-ground. To preserve this safety feature:

•Make sure that the matching wall outlet receptacle is properly wired and earth-grounded. Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the centrifuge.

•Never use a three-to-two wire plug adapter.

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Safety Notice

Safety Against Risk of Fire

•Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet receptacle strip.

Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into the centrifuge and damage electrical or mechanical components.

Safety Against Risk of Fire

This centrifuge is not designed for use with materials capable of developing flammable or explosive vapors or hazardous chemical reactions. Do not centrifuge such materials (such as chloroform or ethyl alcohol) in this centrifuge nor handle or store them within the 30-cm (1-ft) area surrounding the centrifuge.

Mechanical Safety

For safe operation of the equipment, observe the following:

•Use only the rotors and accessories designed for use in this centrifuge.

•Do not exceed the maximum rated speed of the rotor in use.

•NEVER attempt to slow or stop the rotor by hand.

•Do not lift or move the centrifuge while the rotor is spinning.

•NEVER attempt to override the door interlock system while the rotor is spinning.

•Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it is running. During operation you should come within the envelope only to adjust instrument controls, if necessary. Never bring any flammable substances within the 30-cm (1-ft) area surrounding the centrifuge. Never lean on the centrifuge or place items on the centrifuge while it is operating.

Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Such materials should not be used in this centrifuge, however, unless all necessary safety precautions are taken.

•Observe all cautionary information printed on the original solution containers prior to their use.

•Handle body fluids with care because they can transmit disease. No known test offers complete assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize the need for aerosol protection. Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe proper safety precautions for aerosol containment.

Do not run toxic, pathogenic, or radioactive materials in this centrifuge without taking

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Safety Notice

RoHS Notice

appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection.

•Dispose of all waste solutions according to appropriate environmental health and safety guidelines.

It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter.

RoHS Notice

These labels and materials declaration table (the Table of Hazardous Substance's Name and Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006 “Marking for Control of Pollution Caused by Electronic Information Products” requirements.

China RoHS Caution Label

This label indicates that the electronic information product contains certain toxic or hazardous substances. The center number is the Environmentally Friendly Useful Period (EFUP) date, and indicates the number of calendar years the product can be in operation. Upon the expiration of the EFUP, the product must be immediately recycled. The circling arrows indicate the product is recyclable. The date code on the label or product indicates the date of manufacture.

China RoHS Environmental Label

This label indicates that the electronic information product does not contain any toxic or hazardous substances. The center ‘e’ indicates the product is environmentally safe and does not have an Environmentally Friendly Useful Period (EFUP) date. Therefore, it can safely be used indefinitely. The circling arrows indicate the product is recyclable. The date code on the label or product indicates the date of manufacture.

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Safety Notice

RoHS Notice

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Safety Notice, iii

Introduction, xiii

CHAPTER 1: Description, 1-1

Introduction, 1-1

Centrifuge Function and Safety Features, 1-1

Centrifuge Function, 1-1

Safety Features, 1-2

Centrifuge Chassis, 1-2

Housing, 1-2

Door, 1-3

Rotor Chamber, 1-3

Temperature Sensing and Control, 1-3

Drive, 1-3

Name Rating Plate, 1-3

Controls and Indicators, 1-3

Power Switch, 1-3

Control Panel, 1-4

Digital Displays, 1-4

Run-Parameter Keys, 1-5

Specifications, 1-7

Available Rotors, 1-9

CHAPTER 2: Operation, 2-1

Introduction, 2-1

Summary of Run Procedures, 2-2

Manual Run, 2-2

Programmed Run, 2-3

Preparation, 2-3

Installing the Rotor, 2-3

Manual Operation, 2-4

Selecting a Rotor, 2-5

vii

Contents

Entering Run Speed, 2-5

Entering Run Time, 2-5

Entering Run Temperature, 2-6

Entering Acceleration and Deceleration Rates, 2-6

Starting a Run, 2-7

Pulse Function, 2-8

Programmed Operation, 2-9

Creating a New Program, 2-9

Recalling a Program, 2-9

Disabling/Enabling the Audible Tones, 2-10

CHAPTER 3: Troubleshooting, 3-1

Introduction, 3-1

User Messages, 3-1

Illustrations

1.1The Centrifuge, 1-2

1.2Control Panel, 1-4

3.1Door Latch Override, 3-6

A.1	Centrifuge Dimensions, A-2

Tables

2.1Acceleration and Deceleration Rates (in Minutes:Seconds) to

and from Maximum Speed, 2-7

3.1Diagnostic Message Chart, 3-2

3.2Troubleshooting Chart, 3-5

Tables

xii

Introduction

Certification

Beckman Coulter Allegra X-15R centrifuges are manufactured in a facility that maintains certifications to both ISO 9001:2008 and ISO 13485:2003. They have been designed and tested to be compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable regulatory agencies. Declarations of conformity and certificates of compliance are available at www.beckmancoulter.com.

Scope of Manual

This manual is designed to familiarize you with the Beckman Coulter Allegra X-15R centrifuge, its functions, specifications, operation, and routine operator care and maintenance. Beckman Coulter recommends that you read this entire manual, especially the Safety Notice and all safety-related information, before operating the centrifuge or performing instrument maintenance.

•CHAPTER 1, Description contains system specifications and a brief physical and functional description of the centrifuge, including the operating controls and indicators.

•CHAPTER 2, Operation contains centrifuge operating procedures.

•CHAPTER 3, Troubleshooting lists diagnostic messages and other possible malfunctions, together with probable causes and suggested corrective actions.

•CHAPTER 4, Care and Maintenance contains procedures for routine operator care and maintenance, as well as a brief list of supplies and replacement parts

•APPENDIX A, Installation provides requirements for preparing laboratory facilities and installing the centrifuge.

NOTE If the centrifuge is used in a manner other than specified in this manual, the safety and performance of this equipment could be impaired. Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual and/or the applicable rotor manual is the sole responsibility of the user.

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Introduction

Conventions

Certain symbols are used in this manual to call out safety-related and other important information. These international symbols may also be displayed on the centrifuge and are reproduced on the inside of the back cover.

Typographic Conventions

Certain typographic conventions are used throughout this manual to distinguish names of user interface components, such as keys and displays.

• Key names (for example, START or ROTOR) appear in capital letters in boldface type.

CFC-Free Centrifugation

To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of Allegra X-15R centrifuges.

Radio Interference

This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with this instruction manual, may cause interference to radio communications. Operation of this equipment in a residential area is likely to cause interference, in which case the user will be required to correct the interference at his own expense.

Canadian Regulations

This equipment does not exceed the Class A limits for radio noise emissions from digital apparatus as set out in the radio interference regulations of the Canadian Department of Communications.

Le présent appareil numérique n’émet pas de bruits radioélectriques dépassant les limites applicables aux appareils numériques de Classe A prescrites dans le reglement sur le brouillage radioelectrique édicté par le Ministère des Communications du Canada.

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Introduction

Recycling Label

A28219-AA

This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE) Directive of the European Union. The presence of this marking on the product indicates:

1.the device was put on the European market after August 13, 2005 and

2.the device is not to be disposed via the municipal waste collection system of any member state of the European Union.

It is very important that customers understand and follow all laws regarding the proper decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.

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Introduction

Recycling Label

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CHAPTER 1 Description

Introduction

This chapter provides a brief physical and functional description of the Beckman Coulter Allegra X-15R centrifuge. The operating controls and indicators are also described; instructions for their use are in CHAPTER 2, Operation. Chemical compatibilities of materials listed in this manual can be found in Chemical Resistances (publication IN-175). Refer to the applicable rotor manuals for rotor descriptions.

Centrifuge Function and Safety Features

Centrifuge Function

The Beckman Coulter Allegra X-15R centrifuge (see Figure 1.1) is a refrigerated benchtop centrifuge that generates centrifugal forces required for a wide variety of applications. Together with the Beckman Coulter rotors designed for use in this centrifuge, the centrifuge applications include:

•Routine processing such as sample preparations, pelleting, extractions, purifications, concentrations, phase separations, receptor binding, and column centrifugations.

•Cell isolation.

•Binding studies and separation of whole blood.

•Processing large numbers of small-volume samples in multiwell plates for concentrating tissueculture cells, cloning and replicate studies, in-vitro cytotoxicity studies, receptor binding, and genetic engineering experimentation.

•Rapid sedimentation of protein precipitates, large particles, and cell debris.

The Allegra X-15R is microprocessor-controlled, providing interactive operation. The instrument design features a brushless asynchronous motor, automatic rotor identification system, program memory that enables repeated run conditions, temperature control system, and a choice of acceleration and deceleration rates. User messages alert the operator to conditions that may need attention.

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