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GX-IM-5
Allegra® X-12 Series
Centrifuges
Instruction Manual
Symbol Simbolo
Symbol
Symbole
Símbolo
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Title |
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Titel |
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Titre |
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Titulo |
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Titolo |
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Dangerous voltage
Gefährliche elektrische Spannung
Courant haute tension
Voltaje peligroso
Pericolo: alta tensione
Attention, consult accompanying documents
Achtung! Begleitpapiere beachten!
Attention, consulter les documents joints
Atención, consulte los documentos adjuntos
Attenzione: consultare le informazioni allegate
On (power)
Ein (Netzverbindung)
Marche (mise sous tension)
Encendido
Acceso (sotto tensione)
Off (power)
Aus (Netzverbindung)
Arrêt (mise hors tension)
Apagado
Spento (fuori tensione)
Protective earth (ground)
Schutzleiteranschluß
Liaison à la terre
Puesta a tierra de protección
Collegamento di protezione a terra
Earth (ground)
Erde
Terre
Tierra
Scarica a terra
!SAFETY NOTICE
This safety notice summarizes information basic to the safe operation of the equipment described in this manual. The international symbol displayed above is a reminder that all safety instructions should be read and understood before installation, operation, maintenance, or repair of this centrifuge. When you see the symbol on other pages, pay special attention to the safety information presented. Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the centrifuge.
Safety During Installation and/or Maintenance
This centrifuge weighs 121 kg (267 lb). Do not attempt to lift or move it without assistance.
Any servicing of this equipment that requires removal of any covers can expose parts that involve the risk of electric shock or personal injury. Make sure that the power switch is off and the centrifuge is disconnected from the main power source, and refer such servicing to qualified personnel.
Do not replace any centrifuge components with parts not specified for use on this instrument.
Electrical Safety
To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to connect the centrifuge to earth-ground. To preserve this safety feature:
•Make sure that the matching wall outlet receptacle is properly wired and earthgrounded. Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the centrifuge.
•Never use a three-to-two wire plug adapter.
•Never use a two-wire extension cord or a two-wire non-grounding type of multipleoutlet receptacle strip.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into the centrifuge and damage electrical or mechanical components.
Safety Against Risk of Fire
This centrifuge is not designed for use with materials capable of developing flammable or explosive vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol) in this centrifuge nor handle or store them within the 30-cm (1-ft) area surrounding the centrifuge.
Mechanical Safety
For safe operation of the equipment, observe the following:
•Use only the rotors and accessories designed for use in this centrifuge.
•Do not exceed the maximum rated speed of the rotor in use.
•NEVER attempt to slow or stop the rotor by hand.
•Do not lift or move the centrifuge while the rotor is spinning.
•NEVER attempt to override the door interlock system while the rotor is spinning.
•Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it is running. During operation you should come within the envelope only to adjust instrument controls, if necessary. Never bring any flammable substances within the 30-cm (1-ft) area surrounding the centrifuge. Never lean on the centrifuge or place items on the centrifuge while it is operating.
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or radioactive. Such materials should not be used in this centrifuge, however, unless all necessary safety precautions are taken.
•Observe all cautionary information printed on the original solution containers prior to their use.
•Handle body fluids with care because they can transmit disease. No known test offers complete assurance that they are free of micro-organisms. Some of the most virulent— Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize the need for aerosol protection. Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease. Because spills may generate aerosols, observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe containment should be used when Risk Group II materials (as identified in the World Health Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more than one level of protection.
•Dispose of all waste solutions according to appropriate environmental health and safety guidelines.
It is your responsibility to decontaminate the centrifuge and accessories before requesting service by Beckman Coulter.
GX-IM-5
January 2009
Allegra® X-12 Series
Centrifuges
Instruction Manual
© 2009 Beckman Coulter, Inc.
Contents
Page
INTRODUCTION
Certification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Scope of Manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Notes, Cautions, and Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Typographic Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi
CFC-Free Centrifugation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi
Radio Interference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii
Canadian Regulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii
Recycling Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii
SECTION 1: DESCRIPTION
Centrifuge Function and Safety Features . . . . . . . . . . . . . . . . . . . . . . . 1-1
Centrifuge Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Safety Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Centrifuge Chassis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Rotor Chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Temperature Sensing and Control . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Drive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
iii
Contents
Page
Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Power Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Name Rating Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-9
Available Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-10
SECTION 2: INSTALLATION
Space and Location Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Securing the Centrifuge to the Bench . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Test Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
SECTION 3: OPERATION
Summary of Run Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Manual Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Programmed Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Installing the Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Manual Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Selecting a Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Entering Run Speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Entering Run Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
Entering Run Temperature (Allegra X-12R Only) . . . . . . . . . . . . 3-6
Entering Acceleration and Deceleration Rates . . . . . . . . . . . . . . . 3-7
Starting a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
Pulse Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
iv
Contents
Page
Programmed Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
Creating a New Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
Recalling a Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
Disabling/Enabling the Audible Tones . . . . . . . . . . . . . . . . . . . . . . . 3-12
SECTION 4: TROUBLESHOOTING
User Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1 Other Possible Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5 Retrieving Your Sample in Case of Power Failure . . . . . . . . . . . . . . . 4-6
SECTION 5: CARE AND MAINTENANCE
Instrument Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Sterilization and Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Circuit Breaker and Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Storage and Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Supply List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Warranty
v
Illustrations
|
|
Page |
Figure 1-1. |
The Centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . |
. 1-2 |
Figure 1-2. |
Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . |
. 1-4 |
Figure 2-1. |
Centrifuge Dimensions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . |
2-2 |
Figure 4-1. |
Door Latch Override . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . |
4-6 |
Tables
Page
Table 3-1. Acceleration and Deceleration Rates (in Minutes:Seconds)
to and from Maximum Speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
Table 4-1. |
Diagnostic Message Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . |
4-2 |
Table 4-2. |
Troubleshooting Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . |
4-5 |
vii
Introduction
CERTIFICATION
To ensure full system quality, Beckman Coulter Allegra® X-12 series centrifuges are manufactured in a registered ISO 9001 or 13485 facility. They have been designed and tested to be compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable regulatory agencies. Declarations of conformity and certificates of compliance are available at www.beckmancoulter.com.
SCOPE OF MANUAL
This manual is designed to familiarize you with the Beckman Coulter Allegra X-12 series centrifuges, their functions, specifications, operation, and routine operator care and maintenance. We recommend that you read this entire manual, especially the SAFETY NOTICE and
all safety-related information, before operating the centrifuge or performing instrument maintenance.
•Section 1 contains system specifications and a brief physical and functional description of the centrifuge, including the operating controls and indicators.
•Section 2 provides requirements for preparing laboratory facilities and installing the centrifuge.
•Section 3 contains centrifuge operating procedures.
•Section 4 lists diagnostic messages and other possible malfunctions, together with probable causes and suggested corrective actions.
ix
Introduction
•Section 5 contains procedures for routine operator care and maintenance, as well as a brief list of supplies and replacement parts.
NOTE
If the centrifuge is used in a manner other than specified in this manual, the safety and performance of this equipment could be impaired.
Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual and/or the applicable rotor manual is the sole responsibility of the user.
CONVENTIONS
Certain symbols are used in this manual to call out safety-related and other important information. These international symbols may also be displayed on the centrifuge and are reproduced and described below and on the inside of the front cover.
NOTES, CAUTIONS, AND WARNINGS
NOTE
Used to call attention to important information that should be followed during installation, use, or servicing of this equipment
!CAUTION
Used to indicate a potentially hazardous situation which, if not avoided, may result in minor or moderate injury and/or mechanical damage. It is also used to alert against unsafe practices.
x
Introduction
!WARNING
Used whenever an action or condition may potentially cause personal injury or loss of life. Mechanical damage may also result.
WARNING
Indicates high voltage or risk of electric shock. Refer servicing of all areas displaying this symbol to service personnel.
TYPOGRAPHIC CONVENTIONS
Certain typographic conventions are used throughout this manual to distinguish names of user interface components, such as keys and displays.
•Key names (for example, [START] or [ROTOR]) appear in capital letters within brackets.
CFC-FREE CENTRIFUGATION
CFC
To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of Allegra X-12 series centrifuges.
xi
Introduction
RADIO INTERFERENCE
This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with this instruction manual, may cause interference to radio communications. Operation of this equipment in a residential area is likely to cause interference, in which case the user will be required to correct the interference at his own expense.
CANADIAN REGULATIONS
This equipment does not exceed the Class A limits for radio noise emissions from digital apparatus as set out in the radio interference regulations of the Canadian Department of Communications.
Le présent appareil numérique n’émet pas de bruits radioélectriques dépassant les limites applicables aux appareils numériques de Classe A prescrites dans le reglement sur le brouillage radioelectrique édicté par le Ministère des Communications du Canada.
RECYCLING LABEL
A28219-AA
Note: On the instrument, the triangle background is yellow rather than gray.
This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE) Directive of the European Union. The presence of this marking on the product indicates:
1)the device was put on the European market after August 13, 2005 and
2)the device is not to be disposed via the municipal waste collection system of any member state of the European Union.
It is very important that customers understand and follow all laws regarding the proper decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.
xii
1
Description
CENTRIFUGE FUNCTION AND SAFETY FEATURES
CENTRIFUGE FUNCTION
The Beckman Coulter Allegra X-12R (refrigerated) or Allegra X-12 (constant controlled temperature) centrifuge (see Figure 1-1) is a benchtop centrifuge that generates centrifugal forces required for
a wide variety of applications. Together with the Beckman Coulter rotors designed for use in this centrifuge, the centrifuge applications include:
•Routine processing such as sample preparations, pelleting, extractions, purifications, concentrations, phase separations, receptor binding, and column centrifugations.
•Cell isolation.
•Binding studies and separation of whole blood.
•Processing large numbers of small-volume samples in multiwell plates for concentrating tissue-culture cells, cloning and replicate studies, in-vitro cytotoxicity studies, receptor binding, and genetic engineering experimentation.
•Rapid sedimentation of protein precipitates, large particles, and cell debris.
The Allegra X-12 and X-12R are microprocessor-controlled, providing interactive operation. The instrument design features a brushless asynchronous motor, automatic rotor identification system, program memory that enables repeated run conditions, temperature control system, and a choice of acceleration and deceleration rates. User messages alert the operator to conditions that may need attention.
1-1
Description
Figure 1-1. The Centrifuge
SAFETY FEATURES
Allegra X-12 series centrifuges have been designed and tested to operate safely indoors at altitudes up to 2 000 m (6 562 ft).
Safety features include the following.
•An electromechanical door lock system prevents operator contact with spinning rotors and prevents run initiation unless the door is shut and locked. The door is locked when a run is in progress and can be opened only when the rotor is stopped and the LED (lightemitting diode) next to the [DOOR] key is lit. If there is a power failure, the door lock can be manually tripped for sample recovery.
•A steel barrier surrounds the rotor chamber to provide full operator protection.
•A rotor identification system prevents the installed rotor from running above its maximum rated speed. During acceleration the microprocessor checks the magnetic rotor identification. Speed is limited to the maximum safe speed of the identified rotor. If the system identifies a rotor different than the one entered by the user, the system displays an error message and reduces the speed to the maximum allowable speed of the rotor.
•An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor loads are severely out of balance. At low speeds, an incorrectly loaded rotor can cause imbalance. Rotor instability can also occur if the centrifuge is moved while running, or if it is not resting on a level and secure surface (see Section 4,
TROUBLESHOOTING).
1-2
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