Coulter Allegra 25R
Table of contents
Loading...
®
Allegra® 25R Centrifuge
Instruction Manual
BA- IM- 5
SPEED
RCF
RPM
IMBALANCE
ACCEL
TEMP
TIME
ROTOR
OPEN
DOOR
ENTER
DECEL
PULSE
FAST
STOP
START
STOP
Allegra 25R Centrifuge
ACC/DEC
TEMP °C
TIME
Symbol
Symbol
Symbole
Símbolo
Simbolo
!
Title / Titel / Titre / Titulo / Titolo /
Dangerous voltage
Gefährliche elektrische Spannung
Courant haute tension
Voltaje peligroso
Pericolo: alta tensione
Attention, consult accompanying documents
Achtung! Begleitpapiere beachten!
Attention, consulter les documents joints
Atención, consulte los documentos adjuntos
Attenzione: consultare le informazioni allegate
/
On (power)
Ein (Netzverbindung)
Marche (mise sous tension)
Encendido
Acceso (sotto tensione)
Off (power)
Aus (Netzverbindung)
Arrêt (mise hors tension)
Apagado
Spento (fuori tensione)
Protective earth (ground)
Schutzleiteranschluß
Liaison à la terre
Puesta a tierra de protección
Collegamento di protezione a terra
Earth (ground)
Erde
Terre
Tierra
Scarica a terra
!
This safety notice summarizes information basic to the safe operation of the equipment
described in this manual. The international symbol displayed above is a reminder that all
safety instructions should be read and understood before installation, operation, maintenance, or repair of this centrifuge. When you see the symbol on other pages, pay special
attention to the safety information presented. Observance of safety precautions will also
help to avoid actions that could damage or adversely affect the performance of the
centrifuge.
Safety During Installation and/or Maintenance
This centrifuge is designed to be installed by a Beckman Coulter Field Service representative. Installation by anyone other than authorized Beckman Coulter personnel invalidates
any warranty covering the instrument.
This centrifuge weighs 124.0 kg (273.4 lb). Do not attempt to lift or move it without
assistance.
Any servicing of this equipment that requires removal of any covers can expose parts that
involve the risk of electric shock or personal injury. Make sure that the power switch is off
and the centrifuge is disconnected from the main power source, and refer such servicing to
qualified personnel.
SAFETY NOTICE
Do not replace any centrifuge comonents with parts not specified for use on this instrument.
Electrical Safety
To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and
plug to connect the centrifuge to earth-ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earthgrounded. Check that the line voltage agrees with the voltage listed on the name-rating
plate affixed to the centrifuge.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of multipleoutlet receptacle strip.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid
may get into the centrifuge and damage electrical or mechanical components.
Safety Against Risk of Fire
This centrifuge is not designed for use with materials capable of developing flammable or
explosive vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol)
in this centrifuge nor handle or store them within the 30-cm (1-ft) area surrounding the
centrifuge.
Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the rotors and accessories designed for use in this centrifuge.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop the rotor by hand.
• Do not lift or move the centrifuge while the rotor is spinning.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it is running.
During operation you should come within the envelope only to adjust instrument
controls, if necessary. Never bring any flammable substances within the 30-cm (1-ft)
area surrounding the centrifuge. Never lean on the centrifuge or place items on the
centrifuge while it is operating.
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic,
toxic, or radioactive. Such materials should not be used in this centrifuge, however, unless
all necessary safety precautions are taken.
• Observe all cautionary information printed on the original solution containers prior to
their use.
• Handle body fluids with care because they can transmit disease. No known test offers
complete assurance that they are free of micro-organisms. Some of the most virulent—
Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic
fungi—further emphasize the need for aerosol protection. Handle other infectious
samples according to good laboratory procedures and methods to prevent spread of
disease. Because spills may generate aerosols, observe proper safety precautions for
aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this
centrifuge without taking appropriate safety precautions. Biosafe containment should
be used when Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual) are handled; materials of a higher group require more
than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the centrifuge and accessories before
requesting service by Beckman Coulter Field Service.
Allegra® 25R Centrifuge
Instruction Manual
BA-IM-5
April 2009
© 2009 Beckman Coulter, Inc.
SPEED
RCF
RPM
IMBALANCE
ACCEL
TEMP
TIME
ROTOR
OPEN
DOOR
ENTER
DECEL
PULSE
FAST
STOP
START
STOP
Allegra 25R Centrifuge
ACC/DEC
TEMP °C
TIME
INTRODUCTION
Contents
Page
Certification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Scope of Manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
Notes, Cautions, and Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
Typographic Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
CFC-Free Centrifugation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Radio Interference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Canadian Regulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
Recycling Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x
SECTION 1: DESCRIPTION
Centrifuge Function and Safety Features. . . . . . . . . . . . . . . . . . . . . . . 1-1
Centrifuge Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Safety Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Centrifuge Chassis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Rotor Chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Temperature Sensing and Control . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Drive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Power Switch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Name Rating Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
Available Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-9
SECTION 2: PREINSTALLATION REQUIREMENTS
Space and Location Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Transportation Safety Device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
iii
Contents
Page
SECTION 3: OPERATION
Summary of Operating Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Installing the Rotor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Setting Run Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Selecting a Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Setting Run Speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
Setting Run Time. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Setting Run Temperature. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Setting Acceleration and Deceleration Rates . . . . . . . . . . . . . . . . 3-6
Starting a Run. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
Changing Parameters During a Run. . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
Stopping a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
SECTION 4: TROUBLESHOOTING
User Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Other Possible Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Retrieving Your Sample in Case of Power Failure . . . . . . . . . . . . . . . 4-4
SECTION 5: CARE AND MAINTENANCE
General Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Sterilization and Disinfection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Storage and Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Supply List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Warranty
iv
Illustrations
Page
Figure 1-1. The Allegra 25R Centrifuge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Figure 1-2. Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Figure 2-1. Centrifuge Dimensions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Figure 2-2. North American Electrical Socket . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Figure 4-1. Door Latch Override . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Contents
Tabl es
Page
Table 3-1. Acceleration and Deceleration Rates (in Minutes:Seconds)
to and from Maximum Speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
Table 4-1. Error Messages. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Table 4-2. Troubleshooting Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
v
CERTIFICATION
Introduction
To ensure full system quality, Beckman Coulter Allegra® 25R centrifuges have been manufactured in a registered ISO 9001 or 13485
facility. They have been designed and tested to be compliant (when
used with Beckman Coulter rotors) with the laboratory equipment
requirements of applicable regulatory agencies. Declarations of
conformity and certificates of compliance are available at
www.beckmancoulter.com.
SCOPE OF MANUAL
This manual is designed to familiarize you with the Beckman Coulter
Allegra 25R centrifuge, its functions, specifications, operation, and
routine operator care and maintenance. We recommend that you read
this entire manual, especially the SAFETY NOTICE and all safetyrelated information, before operating the centrifuge or performing
instrument maintenance.
• Section 1 contains system specifications and a brief physical and
functional description of the centrifuge, including the operating
controls and indicators.
• Section 2 provides requirements for preparing laboratory facilities
for the centrifuge.
• Section 3 contains centrifuge operating procedures.
• Section 4 lists possible malfunctions, together with probable
causes and suggested corrective actions.
• Section 5 contains procedures for routine operator care and maintenance, as well as a brief list of supplies and replacement parts.
vii
Introduction
CONVENTIONS
➠
NOTE
If the centrifuge is used in a manner other than
specified in this manual, the safety and performance of this equipment could be impaired.
Further, the use of any equipment other than
that recommended by Beckman Coulter has not
been evaluated for safety. Use of any equipment
not specifically recommended in this manual
and/or the appropriate rotor manual is the sole
responsibility of the user.
Certain symbols are used in this manual to call out safety-related and
other important information. These international symbols may also be
displayed on the centrifuge and are reproduced and described below
and on the inside of the front cover.
NOTES, CAUTIONS, AND WARNINGS
➠
!
NOTE
Used to call attention to important information
that should be followed during installation, use,
or servicing of this equipment
CAUTION
Used to indicate a potentially hazardous situation which, if not avoided, may result in minor or
moderate injury and/or mechanical damage. It
is also used to alert against unsafe practices.
viii
!
WARNING
WARNING
Introduction
Used whenever an action or condition may
potentially cause personal injury or loss of
life. Mechanical damage may also result.
Indicates high voltage or risk of electric
shock. Refer servicing of all areas displaying
this symbol to service personnel.
TYPOGRAPHIC CONVENTIONS
Certain typographic conventions are used throughout this manual to
distinguish names of user interface components, such as keys and
displays.
• Key names (for example, [
letters within brackets.
• Display names (for example, SPEED or TIME) appear in
bold type.
CFC-FREE CENTRIFUGATION
To ensure minimal environmental impact, no CFCs are used in the
manufacture or operation of Allegra 25R centrifuges.
CFC
START] or [ROTOR]) appear in capital
ix
Introduction
RADIO INTERFERENCE
CANADIAN REGULATIONS
This equipment has been tested and found to comply with the limits
for a Class A digital device, pursuant to Part 15 of FCC Rules. These
limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial
environment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with
this instruction manual, may cause interference to radio communications. Operation of this equipment in a residential area is likely to
cause interference, in which case the user will be required to correct
the interference at his own expense.
This equipment does not exceed the Class A limits for radio noise
emissions from digital apparatus as set out in the radio interference
regulations of the Canadian Department of Communications.
RECYCLING LABEL
A28219-AA
Note: On the instrument, the
triangle background is yellow
rather than gray.
Le présent appareil numérique n’émet pas de bruits radioélectriques
dépassant les limites applicables aux appareils numériques de Classe
A prescrites dans le reglement sur le brouillage radioelectrique édicté
par le Ministère des Communications du Canada.
This symbol is required in accordance with the Waste Electrical and
Electronic Equipment (WEEE) Directive of the European Union. The
presence of this marking on the product indicates:
1) The device was put on the European market after August 13, 2005
and
2) The device is not to be disposed via the municipal waste collection
system of any member state of the European Union.
It is very important that customers understand and follow all laws
regarding the proper decontamination and safe disposal of electrical
equipment. For Beckman Coulter products bearing this label please
contact your dealer or local Beckman Coulter office for details on the
take back program that will facilitate the proper collection, treatment,
recovery, recycling and safe disposal of the device.
x
CENTRIFUGE FUNCTION AND SAFETY FEATURES
CENTRIFUGE FUNCTION
1
Description
The Beckman Coulter Allegra 25R centrifuge (see Figure 1-1) is a
refrigerated benchtop centrifuge that generates centrifugal forces
required for a wide variety of applications. Together with the
Beckman Coulter rotors designed for use in this centrifuge, the
centrifuge applications include:
• Routine processing such as sample preparations, pelleting, extractions, purifications, concentrations, phase separations, receptor
binding, and column centrifugations.
• Processing large numbers of small-volume samples in multiwell
plates for concentrating tissue-culture cells, cloning and replicate
studies, in-vitro cytotoxicity studies, receptor binding, and genetic
engineering experimentation.
• Rapid sedimentation of protein precipitates, large particles, and
cell debris.
• Binding studies and separation of whole blood.
• Cell isolation.
The Allegra 25R is microprocessor-controlled, providing interactive
operation. The instrument design features a brushless asynchronous
motor, automatic rotor identification system, temperature control
system, and a choice of acceleration and deceleration rates. User
messages alert the operator to conditions that may need attention.
1-1
Description
ACC/DEC
TEMP°C
TIME
SPEED
ACCEL
TEMP
RPM
IMBALANCE
TIME
ROTOR
RCF
OPEN
DOOR
ENTER
DECEL
PULSE
T
FAS
STOP
START
STOP
Figure 1-1. The Allegra 25R Centrifuge
SAFETY FEATURES
The centrifuge has been designed and tested to operate safely indoors
at altitudes up to 2 000 m (6 562 ft). Safety features include the
following.
• An electromechanical door lock system prevents operator contact
with spinning rotors and prevents run initiation unless the door is
shut and locked. The door is locked when a run is in progress and
can be opened only when the rotor is stopped and the LED (lightemitting diode) on the [
OPEN DOOR] key is lit.
• An imbalance detector monitors the rotor during the run, causing
automatic shutdown if rotor loads are severely out of balance. At
low speeds, an incorrectly loaded rotor can cause imbalance. Rotor
instability can also occur if the centrifuge is moved while running,
or if it is not resting on a level and secure surface.
• To prevent overspeed the microprocessor checks the rotor identification during start-up and stops the run if the rotor is incorrectly
identified.
1-2
CENTRIFUGE CHASSIS
HOUSING
DOOR
The centrifuge housing is made of sheet steel and is finished with
urethane paint. The control panel overlay is made of coated
polycarbonate.
The steel door is secured to the housing by solid hinges. When the
door is closed, a motorized locking system engages. The door is
locked when a run is in progress and can be opened only when the
rotor is stopped and the LED on the [
is a power failure, the door lock can be manually tripped for sample
recovery (see Section 4, Troubleshooting).
OPEN DOOR] key is lit. If there
Description
ROTOR CHAMBER
The rotor chamber is stainless steel for durability and corrosion
resistance. The chamber is sealed by a silicone rubber gasket.
TEMPERATURE SENSING AND CONTROL
With the power on, the temperature control system is activated when
the door is closed and locked. Run temperature can be set between
–20 and +40°C. If no set temperature is entered, the instrument automatically selects the last entered temperature.
A sensor in the rotor chamber continuously monitors chamber
temperature. The microprocessor calculates the required chamber
temperature to maintain the selected rotor temperature.
NOTE
➠
To avoid chamber icing, refrigeration is off
when the door is open. The centrifuge door must
be closed and locked for the refrigeration system
to begin operating.
1-3
Loading...