Beckman Coulter Allegra X-15R User manual
Instructions For Use
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
Allegra X-15R
Centrifuge
PN GXR-IM-4AC
October 2013
Allegra X-15R
Centrifuge
PN GXR-IM-4AC (October 2013)
© 2013 Beckman Coulter, Inc.
All rights reserved. No part of this document may be
reproduced or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or
otherwise, without prior written permission from
Beckman Coulter, Inc.
Beckman Coulter, the stylized logo, and Allegra are
trademarks of Beckman Coulter, Inc. and are registered in
the USPTO.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Find us on the World Wide Web at:
www.beckmancoulter.com
Made in U.S.A.
Printed in U.S.A.
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact your Beckman Coulter Representative.
Alerts for Warning, Caution, and Note
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury and/or mechanical damage.
Safety Notice
NOTE
NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
Safety During Installation and/or Maintenance
This centrifuge weighs 128 kg (283 lb). Do not attempt to lift or move it without assistance.
Any servicing of this equipment that requires removal of any covers can expose parts that involve
the risk of electric shock or personal injury. Make sure that the power switch is off and the
centrifuge is disconnected from the main power source by removing the Mains (power) plug from
the outlet receptacle, and refer such servicing to qualified personnel.
Do not replace any centrifuge components with parts not specified for use on this instrument.
Electrical Safety
To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to
connect the centrifuge to earth-ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to
the centrifuge.
• Never use a three-to-two wire plug adapter.
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Safety Notice
Safety Against Risk of Fire
• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet
receptacle strip.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into
the centrifuge and damage electrical or mechanical components.
Safety Against Risk of Fire
This centrifuge is not designed for use with materials capable of developing flammable or explosive
vapors or hazardous chemical reactions. Do not centrifuge such materials (such as chloroform or
ethyl alcohol) in this centrifuge nor handle or store them within the 30-cm (1-ft) area surrounding
the centrifuge.
Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the rotors and accessories designed for use in this centrifuge.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop the rotor by hand.
• Do not lift or move the centrifuge while the rotor is spinning.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it is running. During
operation you should come within the envelope only to adjust instrument controls, if
necessary. Never bring any flammable substances within the 30-cm (1-ft) area surrounding the
centrifuge. Never lean on the centrifuge or place items on the centrifuge while it is operating.
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this centrifuge, however, unless all necessary
safety precautions are taken.
• Observe all cautionary information printed on the original solution containers prior to
their use.
• Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize
the need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment.
Do not run toxic, pathogenic, or radioactive materials in this centrifuge without taking
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PN GXR-IM-4AC
appropriate safety precautions. Biosafe containment should be used when Risk Group II
materials (as identified in the World Health Organization Laboratory Biosafety Manual) are
handled; materials of a higher group require more than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the centrifuge and accessories before requesting service by
Beckman Coulter.
RoHS Notice
These labels and materials declaration table (the Table of Hazardous Substance's Name and
Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.
China RoHS Caution Label
Safety Notice
RoHS Notice
This label indicates that the electronic information product contains certain toxic or hazardous
substances. The center number is the Environmentally Friendly Useful Period (EFUP) date, and
indicates the number of calendar years the product can be in operation. Upon the expiration of the
EFUP, the product must be immediately recycled. The circling arrows indicate the product is
recyclable. The date code on the label or product indicates the date of manufacture.
China RoHS Environmental Label
This label indicates that the electronic information product does not contain any toxic or hazardous
substances. The center ‘e’ indicates the product is environmentally safe and does not have an
Environmentally Friendly Useful Period (EFUP) date. Therefore, it can safely be used indefinitely.
The circling arrows indicate the product is recyclable. The date code on the label or product
indicates the date of manufacture.
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Safety Notice
RoHS Notice
vi
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Safety Notice,iii
Introduction,xiii
CHAPTER 1: Description,1-1
Introduction,1-1
Centrifuge Function and Safety Features,1-1
Centrifuge Function,1-1
Safety Features,1-2
Contents
Centrifuge Chassis,1-2
Housing,1-2
Door,1-3
Rotor Chamber,1-3
Temperature Sensing and Control,1-3
Drive,1-3
Name Rating Plate,1-3
Controls and Indicators,1-3
Power Switch,1-3
Control Panel,1-4
Digital Displays,1-4
Run-Parameter Keys,1-5
Specifications,1-7
Available Rotors,1-9
CHAPTER 2: Operation,2-1
Introduction,2-1
Summary of Run Procedures,2-2
Manual Run,2-2
Programmed Run,2-3
Preparation,2-3
Installing the Rotor,2-3
Manual Operation,2-4
Selecting a Rotor,2-5
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Contents
Entering Run Speed,2-5
Entering Run Time,2-5
Entering Run Temperature,2-6
Entering Acceleration and Deceleration Rates,2-6
Starting a Run,2-7
Pulse Function,2-8
Programmed Operation,2-9
Creating a New Program,2-9
Recalling a Program,2-9
Disabling/Enabling the Audible Tones,2-10
CHAPTER 3: Troubleshooting,3-1
Introduction,3-1
User Messages,3-1
Other Possible Problems,3-4
Retrieving Your Sample in Case of Power Failure,3-6
CHAPTER 4: Care and Maintenance,4-1
Introduction,4-1
Instrument Care,4-1
Maintenance,4-1
Cleaning,4-2
Tube Breakage,4-2
Decontamination,4-3
Sterilization and Disinfection,4-3
Circuit Breaker and Fuses,4-3
Storage and Transport,4-4
Storage,4-4
Supply List,4-4
Replacement Rotor Parts,4-4
Other,4-4
APPENDIX A: Installation,A-1
Introduction,A-1
viii
Space and Location Requirements,A-1
Electrical Requirements,A-2
Securing the Centrifuge to the Bench,A-3
Test Run,A-4
Beckman Coulter, Inc.
Allegra X-15R Centrifuge Warranty
Related Documents
Contents
ix
Illustrations
Illustrations
1.1 The Centrifuge,1-2
1.2 Control Panel,1-4
3.1 Door Latch Override,3-6
A.1 Centrifuge Dimensions,A-2
x
Tables
2.1 Acceleration and Deceleration Rates (in Minutes:Seconds) to
and from Maximum Speed,2-7
3.1 Diagnostic Message Chart,3-2
3.2 Troubleshooting Chart,3-5
Tables
xi
Tables
xii
Certification
Beckman Coulter Allegra X-15R centrifuges are manufactured in a facility that maintains
certifications to both ISO 9001:2008 and ISO 13485:2003. They have been designed and tested to be
compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements
of applicable regulatory agencies. Declarations of conformity and certificates of compliance are
available at www.beckmancoulter.com.
Introduction
Scope of Manual
This manual is designed to familiarize you with the Beckman Coulter Allegra X-15R centrifuge, its
functions, specifications, operation, and routine operator care and maintenance. Beckman Coulter
recommends that you read this entire manual, especially the Safety Notice and all safety-related
information, before operating the centrifuge or performing instrument maintenance.
• CHAPTER 1, Description contains system specifications and a brief physical and functional
description of the centrifuge, including the operating controls and indicators.
• CHAPTER 2, Operation contains centrifuge operating procedures.
• CHAPTER 3, Troubleshooting lists diagnostic messages and other possible malfunctions,
together with probable causes and suggested corrective actions.
• CHAPTER 4, Care and Maintenance contains procedures for routine operator care and
maintenance, as well as a brief list of supplies and replacement parts
• APPENDIX A, Installation provides requirements for preparing laboratory facilities and
installing the centrifuge.
NOTE
If the centrifuge is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that recommended
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual and/or the applicable rotor manual is the sole responsibility of the user.
PN GXR-IM-4AC
xiii
Introduction
Conventions
Conventions
Certain symbols are used in this manual to call out safety-related and other important information.
These international symbols may also be displayed on the centrifuge and are reproduced on the
inside of the back cover.
Typographic Conventions
Certain typographic conventions are used throughout this manual to distinguish names of user
interface components, such as keys and displays.
• Key names (for example,
CFC-Free Centrifugation
To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of
Allegra X-15R centrifuges.
Radio Interference
This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of FCC Rules. These limits are designed to provide reasonable protection against
harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with this instruction manual, may cause interference to radio communications.
Operation of this equipment in a residential area is likely to cause interference, in which case the
user will be required to correct the interference at his own expense.
Canadian Regulations
START or ROTOR) appear in capital letters in boldface type.
xiv
This equipment does not exceed the Class A limits for radio noise emissions from digital apparatus
as set out in the radio interference regulations of the Canadian Department of Communications.
Le présent appareil numérique n’émet pas de bruits radioélectriques dépassant les limites
applicables aux appareils numériques de Classe A prescrites dans le reglement sur le brouillage
radioelectrique édicté par le Ministère des Communications du Canada.
PN GXR-IM-4AC
Recycling Label
A28219-AA
This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:
1. the device was put on the European market after August 13, 2005 and
2. the device is not to be disposed via the municipal waste collection system of any member state
of the European Union.
It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label please contact your dealer or local Beckman Coulter office for details on the take back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.
Introduction
Recycling Label
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Introduction
Recycling Label
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PN GXR-IM-4AC
Introduction
This chapter provides a brief physical and functional description of the Beckman Coulter Allegra X-15R
centrifuge. The operating controls and indicators are also described; instructions for their use are in
CHAPTER 2, Operation. Chemical compatibilities of materials listed in this manual can be found in
Chemical Resistances (publication IN-175). Refer to the applicable rotor manuals for rotor descriptions.
Centrifuge Function and Safety Features
CHAPTER 1
Description
Centrifuge Function
The Beckman Coulter Allegra X-15R centrifuge (see Figure 1.1) is a refrigerated benchtop centrifuge
that generates centrifugal forces required for a wide variety of applications. Together with the
Beckman Coulter rotors designed for use in this centrifuge, the centrifuge applications include:
• Routine processing such as sample preparations, pelleting, extractions, purifications,
concentrations, phase separations, receptor binding, and column centrifugations.
• Cell isolation.
• Binding studies and separation of whole blood.
• Processing large numbers of small-volume samples in multiwell plates for concentrating tissue-
culture cells, cloning and replicate studies, in-vitro cytotoxicity studies, receptor binding, and
genetic engineering experimentation.
• Rapid sedimentation of protein precipitates, large particles, and cell debris.
The Allegra X-15R is microprocessor-controlled, providing interactive operation. The instrument
design features a brushless asynchronous motor, automatic rotor identification system, program
memory that enables repeated run conditions, temperature control system, and a choice of
acceleration and deceleration rates. User messages alert the operator to conditions that may need
attention.
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