Beckman Coulter Allegra X-12 User manual

GX- IM- 5

Allegra® X-12 Series
Centrifuges

Instruction Manual

Symbol
Symbol
Symbole
Símbolo

Simbolo

!

Title / Titel / Titre / Titulo / Titolo /

Dangerous voltage
Gefährliche elektrische Spannung
Courant haute tension
Voltaje peligroso
Pericolo: alta tensione

Attention, consult accompanying documents
Achtung! Begleitpapiere beachten!
Attention, consulter les documents joints
Atención, consulte los documentos adjuntos
Attenzione: consultare le informazioni allegate

/

On (power)
Ein (Netzverbindung)
Marche (mise sous tension)
Encendido
Acceso (sotto tensione)

Off (power)
Aus (Netzverbindung)
Arrêt (mise hors tension)
Apagado
Spento (fuori tensione)

Protective earth (ground)
Schutzleiteranschluß
Liaison à la terre
Puesta a tierra de protección
Collegamento di protezione a terra

Earth (ground)
Erde
Terre
Tierra
Scarica a terra

!

This safety notice summarizes information basic to the safe operation of the equipment
described in this manual. The international symbol displayed above is a reminder that all
safety instructions should be read and understood before installation, operation, mainte­nance, or repair of this centrifuge. When you see the symbol on other pages, pay special
attention to the safety information presented. Observance of safety precautions will also
help to avoid actions that could damage or adversely affect the performance of the
centrifuge.

Safety During Installation and/or Maintenance

This centrifuge weighs 121 kg (267 lb). Do not attempt to lift or move it without
assistance.

Any servicing of this equipment that requires removal of any covers can expose parts that
involve the risk of electric shock or personal injury. Make sure that the power switch is off
and the centrifuge is disconnected from the main power source, and refer such servicing to
qualified personnel.

SAFETY NOTICE

Do not replace any centrifuge components with parts not specified for use on this
instrument.

Electrical Safety

To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and
plug to connect the centrifuge to earth-ground. To preserve this safety feature:

• Make sure that the matching wall outlet receptacle is properly wired and earth­grounded. Check that the line voltage agrees with the voltage listed on the name-rating
plate affixed to the centrifuge.

• Never use a three-to-two wire plug adapter.

• Never use a two-wire extension cord or a two-wire non-grounding type of multiple­outlet receptacle strip.

Do not place containers holding liquid on or near the chamber door. If they spill, liquid
may get into the centrifuge and damage electrical or mechanical components.

Safety Against Risk of Fire

This centrifuge is not designed for use with materials capable of developing flammable or
explosive vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol)
in this centrifuge nor handle or store them within the 30-cm (1-ft) area surrounding the
centrifuge.

Mechanical Safety

For safe operation of the equipment, observe the following:

• Use only the rotors and accessories designed for use in this centrifuge.

• Do not exceed the maximum rated speed of the rotor in use.

• NEVER attempt to slow or stop the rotor by hand.

• Do not lift or move the centrifuge while the rotor is spinning.

• NEVER attempt to override the door interlock system while the rotor is spinning.

• Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it is running.
During operation you should come within the envelope only to adjust instrument
controls, if necessary. Never bring any flammable substances within the 30-cm (1-ft)
area surrounding the centrifuge. Never lean on the centrifuge or place items on the
centrifuge while it is operating.

Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic,
toxic, or radioactive. Such materials should not be used in this centrifuge, however, unless

all necessary safety precautions are taken.

• Observe all cautionary information printed on the original solution containers prior to
their use.

• Handle body fluids with care because they can transmit disease. No known test offers
complete assurance that they are free of micro-organisms. Some of the most virulent—
Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic
fungi—further emphasize the need for aerosol protection. Handle other infectious
samples according to good laboratory procedures and methods to prevent spread of
disease. Because spills may generate aerosols, observe proper safety precautions for
aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this
centrifuge without taking appropriate safety precautions. Biosafe containment should
be used when Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual) are handled; materials of a higher group require more
than one level of protection.

• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.

It is your responsibility to decontaminate the centrifuge and accessories before
requesting service by Beckman Coulter.

GX-IM-5

A

©

January 2009

llegra® X-12 Series

Centrifuges

Instruction Manual

2009 Beckman Coulter, Inc.

INTRODUCTION

Contents

Page

Certification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix

Scope of Manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix

Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x

Notes, Cautions, and Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . x

Typographic Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi

CFC-Free Centrifugation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi

Radio Interference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii

Canadian Regulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii

Recycling Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii

SECTION 1: DESCRIPTION

Centrifuge Function and Safety Features. . . . . . . . . . . . . . . . . . . . . . . 1-1

Centrifuge Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1

Safety Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2

Centrifuge Chassis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3

Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3

Door . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3

Rotor Chamber. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3

Temperature Sensing and Control . . . . . . . . . . . . . . . . . . . . . . . . . 1-3

Drive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4

iii

Contents

Page

Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4

Power Switch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4

Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4

Name Rating Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8

Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-9

Available Rotors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-10

SECTION 2: INSTALLATION

Space and Location Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1

Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

Securing the Centrifuge to the Bench . . . . . . . . . . . . . . . . . . . . . . . . . 2-3

Test Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4

SECTION 3: OPERATION

Summary of Run Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

Manual Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2

Programmed Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

Installing the Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3

Manual Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4

Selecting a Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5

Entering Run Speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5

Entering Run Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6

Entering Run Temperature (Allegra X-12R Only) . . . . . . . . . . . . 3-6

Entering Acceleration and Deceleration Rates . . . . . . . . . . . . . . . 3-7

Starting a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8

Pulse Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9

iv

Programmed Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10

Creating a New Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10

Recalling a Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11

Disabling/Enabling the Audible Tones . . . . . . . . . . . . . . . . . . . . . . . 3-12

SECTION 4: TROUBLESHOOTING

User Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1

Other Possible Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5

Retrieving Your Sample in Case of Power Failure . . . . . . . . . . . . . . . 4-6

Contents

Page

SECTION 5: CARE AND MAINTENANCE

Instrument Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1

Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2

Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2

Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3

Sterilization and Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3

Circuit Breaker and Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4

Storage and Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4

Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4

Supply List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5

Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5

Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5

Warranty

v

Illustrations

Page

Figure 1-1. The Centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2

Figure 1-2. Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4

Figure 2-1. Centrifuge Dimensions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2

Figure 4-1. Door Latch Override . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6

Tabl es

Page

Table 3-1. Acceleration and Deceleration Rates (in Minutes:Seconds)

to and from Maximum Speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8

Table 4-1. Diagnostic Message Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2

Table 4-2. Troubleshooting Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5

vii

CERTIFICATION

Introduction

To ensure full system quality, Beckman Coulter Allegra® X-12 series
centrifuges are manufactured in a registered ISO 9001 or 13485
facility. They have been designed and tested to be compliant (when
used with Beckman Coulter rotors) with the laboratory equipment
requirements of applicable regulatory agencies. Declarations of
conformity and certificates of compliance are available at
www.beckmancoulter.com.

SCOPE OF MANUAL

This manual is designed to familiarize you with the Beckman Coulter
Allegra X-12 series centrifuges, their functions, specifications, opera­tion, and routine operator care and maintenance. We recommend that
you read this entire manual, especially the
all safety-related information, before operating the centrifuge or
performing instrument maintenance.

• Section 1 contains system specifications and a brief physical and
functional description of the centrifuge, including the operating
controls and indicators.

• Section 2 provides requirements for preparing laboratory facilities
and installing the centrifuge.

• Section 3 contains centrifuge operating procedures.

• Section 4 lists diagnostic messages and other possible malfunc­tions, together with probable causes and suggested corrective
actions.

SAFETY NOTICE and

ix

Introduction

• Section 5 contains procedures for routine operator care and main­tenance, as well as a brief list of supplies and replacement parts.

NOTE

➠

If the centrifuge is used in a manner other than
specified in this manual, the safety and perfor­mance of this equipment could be impaired.
Further, the use of any equipment other than
that recommended by Beckman Coulter has not
been evaluated for safety. Use of any equipment
not specifically recommended in this manual
and/or the applicable rotor manual is the sole
responsibility of the user.

CONVENTIONS

Certain symbols are used in this manual to call out safety-related and
other important information. These international symbols may also be
displayed on the centrifuge and are reproduced and described below
and on the inside of the front cover.

NOTES, CAUTIONS, AND WARNINGS

➠

!

NOTE

Used to call attention to important information
that should be followed during installation, use,
or servicing of this equipment

CAUTION

Used to indicate a potentially hazardous situa­tion which, if not avoided, may result in minor or
moderate injury and/or mechanical damage. It
is also used to alert against unsafe practices.

x

!

WARNING

WAR NING

Introduction

Used whenever an action or condition may
potentially cause personal injury or loss of
life. Mechanical damage may also result.

Indicates high voltage or risk of electric
shock. Refer servicing of all areas displaying
this symbol to service personnel.

TYPOGRAPHIC CONVENTIONS

Certain typographic conventions are used throughout this manual to
distinguish names of user interface components, such as keys and
displays.

• Key names (for example, [
letters within brackets.

CFC-FREE CENTRIFUGATION

To ensure minimal environmental impact, no CFCs are used in the
manufacture or operation of Allegra X-12 series centrifuges.

CFC

START] or [ROTOR]) appear in capital

xi

Introduction

RADIO INTERFERENCE

CANADIAN REGULATIONS

This equipment has been tested and found to comply with the limits
for a Class A digital device, pursuant to Part 15 of FCC Rules. These
limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial environ­ment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with
this instruction manual, may cause interference to radio communica­tions. Operation of this equipment in a residential area is likely to
cause interference, in which case the user will be required to correct
the interference at his own expense.

RECYCLING LABEL

A28219-AA

Note: On the instrument, the
triangle background is yellow
rather than gray.

This equipment does not exceed the Class A limits for radio noise
emissions from digital apparatus as set out in the radio interference
regulations of the Canadian Department of Communications.

Le présent appareil numérique n’émet pas de bruits radioélectriques
dépassant les limites applicables aux appareils numériques de Classe
A prescrites dans le reglement sur le brouillage radioelectrique édicté
par le Ministère des Communications du Canada.

This symbol is required in accordance with the Waste Electrical and
Electronic Equipment (WEEE) Directive of the European Union. The
presence of this marking on the product indicates:

1) the device was put on the European market after August 13, 2005

and

2) the device is not to be disposed via the municipal waste collection

system of any member state of the European Union.

xii

It is very important that customers understand and follow all laws
regarding the proper decontamination and safe disposal of electrical
equipment. For Beckman Coulter products bearing this label please
contact your dealer or local Beckman Coulter office for details on the
take back program that will facilitate the proper collection, treatment,
recovery, recycling and safe disposal of the device.

CENTRIFUGE FUNCTION AND SAFETY FEATURES

CENTRIFUGE FUNCTION

The Beckman Coulter Allegra X-12R (refrigerated) or Allegra X-12
(constant controlled temperature) centrifuge (see Figure 1-1) is a
benchtop centrifuge that generates centrifugal forces required for
a wide variety of applications. Together with the Beckman Coulter
rotors designed for use in this centrifuge, the centrifuge applications
include:

1

Description

• Routine processing such as sample preparations, pelleting, extrac­tions, purifications, concentrations, phase separations, receptor
binding, and column centrifugations.

• Cell isolation.

• Binding studies and separation of whole blood.

• Processing large numbers of small-volume samples in multiwell
plates for concentrating tissue-culture cells, cloning and replicate
studies, in-vitro cytotoxicity studies, receptor binding, and genetic
engineering experimentation.

• Rapid sedimentation of protein precipitates, large particles, and
cell debris.

The Allegra X-12 and X-12R are microprocessor-controlled,
providing interactive operation. The instrument design features a
brushless asynchronous motor, automatic rotor identification system,
program memory that enables repeated run conditions, temperature
control system, and a choice of acceleration and deceleration rates.
User messages alert the operator to conditions that may need
attention.

1-1

Description

Figure 1-1. The Centrifuge

SAFETY FEATURES

Allegra X-12 series centrifuges have been designed and tested to
operate safely indoors at altitudes up to 2 000 m (6 562 ft).

Safety features include the following.

• An electromechanical door lock system prevents operator contact
with spinning rotors and prevents run initiation unless the door is
shut and locked. The door is locked when a run is in progress and
can be opened only when the rotor is stopped and the LED (light­emitting diode) next to the [

DOOR] key is lit. If there is a power

failure, the door lock can be manually tripped for sample recovery.

• A steel barrier surrounds the rotor chamber to provide full operator
protection.

• A rotor identification system prevents the installed rotor from
running above its maximum rated speed. During acceleration the
microprocessor checks the magnetic rotor identification. Speed is
limited to the maximum safe speed of the identified rotor. If the
system identifies a rotor different than the one entered by the user,
the system displays an error message and reduces the speed to the
maximum allowable speed of the rotor.

1-2

• An imbalance detector monitors the rotor during the run, causing
automatic shutdown if rotor loads are severely out of balance. At
low speeds, an incorrectly loaded rotor can cause imbalance. Rotor
instability can also occur if the centrifuge is moved while running,
or if it is not resting on a level and secure surface (see Section 4,

TROUBLESHOOTING).