Dangerous voltage
Gefährliche elektrische Spannung
Courant haute tension
Voltaje peligroso
Pericolo: alta tensione
Attention, consult accompanying documents
Achtung! Begleitpapiere beachten!
Attention, consulter les documents joints
Atención, consulte los documentos adjuntos
Attenzione: consultare le informazioni allegate
/
On (power)
Ein (Netzverbindung)
Marche (mise sous tension)
Encendido
Acceso (sotto tensione)
Off (power)
Aus (Netzverbindung)
Arrêt (mise hors tension)
Apagado
Spento (fuori tensione)
Protective earth (ground)
Schutzleiteranschluß
Liaison à la terre
Puesta a tierra de protección
Collegamento di protezione a terra
Earth (ground)
Erde
Terre
Tierra
Scarica a terra
!
This safety notice summarizes information basic to the safe operation of the equipment
described in this manual. The international symbol displayed above is a reminder that all
safety instructions should be read and understood before installation, operation, mainte
nance, or repair of this centrifuge. When you see the symbol on other pages, pay special
attention to the safety information presented. Observance of safety precautions will also
help to avoid actions that could damage or adversely affect the performance of the
centrifuge.
Safety During Installation and/or Maintenance
This centrifuge weighs 51.7 kg/114.0 lb (nonrefrigerated benchtop), 75.8 kg/167.0 lb
(refrigerated benchtop), or 90.3 kg/199.0 lb (refrigerated kneewell). DO NOT attempt
to
lift or move it without assistance from another person.
Be sure to use the anchoring system to secure the centrifuge in place. The anchoring
system is designed to reduce the possibility of injury or damage that could result from
centrifuge movement in the event of a major rotor mishap.
Any servicing of this equipment that requires removal of any covers can expose parts
which involve the risk of electric shock or personal injury. Make sure that the power
switch is off and the centrifuge is disconnected from the main power source, and refer
such servicing to qualified personnel.
SAFETY NOTICE
-
Do not replace any centrifuge components with parts not specified for use on this
instrument.
Electrical Safety
To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and
plug to connect the centrifuge to earth-ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earthgrounded. Check that the line voltage agrees with the voltage listed on the name-rating
plate affixed to the centrifuge.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of multipleoutlet receptacle strip.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid
may get into the centrifuge and damage electrical or mechanical components.
Safety Against Risk of Fire
Certain electrical circuits within this equipment are protected by fuses against overcurrent
conditions. For continued protection against the risk of fire, replace only with the same
type and rating specified.
This centrifuge is not designed for use with materials capable of developing flammable or
explosive vapors. Do not centrifuge such materials (such as chloroform or ethyl alcohol)
in this centrifuge nor handle or store them within the 30-cm (1-ft) area surrounding the
centrifuge.
Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the rotors and accessories designed for use in this centrifuge.
• Before starting the centrifuge, make sure that the rotor tie-down nut (or rotor lid knob)
is securely fastened.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop the rotor by hand.
• Do not lift or move the centrifuge while the rotor is spinning.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge while it is running.
During operation you should come within the envelope only to adjust instrument
controls, if necessary. Never lean on the centrifuge or place items on the centrifuge
while it is operating.
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic,
toxic, or radioactive. Such materials should not be used in this centrifuge, however, unless
all necessary safety precautions are taken.
• Observe all cautionary information printed on the original solution containers prior to
their use.
• Handle body fluids with care because they can transmit disease. No known test offers
complete assurance that they are free of micro-organisms. Some of the most virulent
Hepatitis (B and C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic
— further emphasize the need for aerosol protection. Handle other infectious
fungi
samples according to good laboratory procedures and methods to prevent spread of
disease. Because spills may generate aerosols, observe proper safety precautions for
aerosol containment. Do not run toxic, pathogenic, or radioactive materials in this
centrifuge without taking appropriate safety precautions. Biosafe containment should
be used when Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual) are handled; materials of a higher group require more
than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
—
It is your responsibility to decontaminate the centrifuge and accessories before
requesting service by Beckman Coulter Field Service.
To ensure full system quality, Beckman Coulter Allegra® 6 series and
Spinchron
13485 facility. They have been designed and tested to be compliant
(when used with Beckman Coulter rotors) with the laboratory equip
ment requirements of applicable regulatory agencies. Declarations
conformity and certificates of compliance are available at
of
www.beckmancoulter.com.
™
R centrifuges have been manufactured in an ISO 9001 or
-
SCOPE OF MANUAL
This manual is designed to familiarize you with the Allegra 6 series
and Spinchron R centrifuges, their functions, specifications, opera
tion, and routine operator care and maintenance. We recommend that
you read this entire manual, especially the
safety-related information, before operating the centrifuge or
all
performing instrument maintenance.
NOTE
➠
The descriptions and instructions are the same
for Allegra 6R and Spinchron R centrifuges.
References in this manual to an Allegra 6R
model also apply to the Spinchron R model.
• Section 1 contains system specifications and a brief physical and
functional description of the centrifuge, including the operating
controls and indicators.
• Section 2 provides instructions for installing and connecting the
centrifuge.
• Section 4 lists possible malfunctions, together with probable
causes and suggested corrective actions.
• Section 5 contains procedures for routine operator care and maintenance, as well as a brief list of supplies and replacement parts.
NOTE
➠
If the centrifuge is used in a manner other
than
specified in this manual, the safety and
performance of this equipment could be
impaired. Further, the use of any equipment
other than that recommended by Beckman
Coulter has not been evaluated for safety. Use of
any equipment not specifically recommended in
this manual and/or the appropriate rotor manual
is the sole responsibility of the user.
CONVENTIONS
Certain symbols are used in this manual to call out safety-related and
other important information. These international symbols may also be
displayed on the centrifuge and are reproduced and described below
and on the inside of the front cover.
NOTES, CAUTIONS, AND WARNINGS
➠
!
NOTE
Used to call attention to important information
that should be followed during installation, use,
or servicing of this equipment.
CAUTION
Used to indicate a potentially hazardous situation which, if not avoided, may result in minor or
moderate injury and/or mechanical damage. It
is also used to alert against unsafe practices.
vi
!
WARNING
WARNING
Introduction
Used whenever an action or condition may
potentially cause personal injury or loss of
life. Mechanical damage may also result.
Indicates high voltage or risk of electric
shock. Refer servicing of all areas displaying
either symbol to service personnel.
CFC-FREE CENTRIFUGATION
To ensure minimal environmental impact, no CFCs are used in the
manufacture or operation of Allegra 6 series and Spinchron R
CFC
centrifuges.
RADIO INTERFERENCE
This equipment has been tested and found to comply with the limits
for a Class A digital device, pursuant to Part 15 of FCC Rules. These
limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial environ
ment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with
this instruction manual, may cause interference to radio communica
tions. Operation of this equipment in a residential area is likely to
cause interference, in which case the user will be required to correct
the interference at his own expense.
-
-
vii
Introduction
CANADIAN REGULATIONS
RECYCLING LABEL
This equipment does not exceed the Class A limits for radio noise
emissions from digital apparatus as set out in the radio interference
regulations of the Canadian Department of Communications.
Le présent appareil numérique n’émet pas de bruits radioélectriques
dépassant les limites applicables aux appareils numériques de Classe
A prescrites dans le reglement sur le brouillage radioelectrique édicté
par le Ministère des Communications du Canada.
This symbol is required in accordance with the Waste Electrical and
Electronic Equipment (WEEE) Directive of the European Union. The
presence of this marking on the product indicates.
A28219-AA
Note: On the instrument, the
triangle background is yellow
rather than gray.
1) The device was put on the European market after August 13, 2005
and
2) The device is not to be disposed via the municipal waste collection
system of any member state of the European Union.
It is very important that customers understand and follow all laws
regarding the proper decontamination and safe disposal of electrical
equipment. For Beckman Coulter products bearing this label please
contact your dealer or local Beckman Coulter office for details on the
take back program that will facilitate the proper collection, treatment,
recovery, recycling and safe disposal of the device.
viii
CENTRIFUGE FUNCTION AND SPECIFICATIONS
CENTRIFUGE FUNCTION
The Beckman Coulter Allegra 6 centrifuge generates centrifugal
forces required for a wide variety of applications. Together with the
Beckman Coulter rotors designed for use in this centrifuge, the centri
fuge applications include:
• Routine processing such as sample preparations, pelleting, extrac-
tions, purifications, concentrations, phase separations, receptor
binding, and column centrifugations.
1
Description
-
• Processing large numbers of small-volume samples in multiwell
plates for concentrating tissue-culture cells, cloning and replicate
studies, in-vitro cytotoxicity studies, receptor binding, and genetic
engineering experimentation.
• Virus isolation.
• Rapid sedimentation of protein precipitates, large particles, and
cell debris.
• Preparation of subcellular organelles such as mitochondria,
granules, and crude microsomes.
• Binding studies and separation of whole blood.
• Cell isolation.
ALLEGRA 6 AND SPINCHRON MODELS
The Allegra 6 (see Figure 1-1) comes in three models and the
Spinchron in one:
• A nonrefrigerated compact bench model (no Spinchron model)
• A refrigerated compact bench model
• A refrigerated kneewell model (no Spinchron model)
1-1
Description
Allegra 6
Allegra 6R
or
Spinchron R
Allegra 6KR
Figure 1-1. Allegra 6 Centrifuges
1-2
Description
SPECIFICATIONS
Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data,
without guarantee.