Agilent Technologies 8000 Operator's Manual

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Agilent 8000 Dissolution Sampling Station

Operator’s Manual

Agilent Technologies

Notices

CAUTION

WARNING

No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.

Manual Part Number

70-9060

Edition

Rev C, May 2014

Agilent Technologies, Inc. 3501 Stevens Creek Blvd. Santa Clara, CA 95052 USA

Warranty

The material contained in this document is provided “as is,” and is subject to being changed, without notice, in future editions. Further, to the maximum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a particular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or performance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the separate agreement shall control.

Technology Licenses

The hardware and/or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license.

Restricted Rights Legend

U.S. Government Restricted Rights. Software and technical data rights granted to the federal government include only those rights customarily provided to end user customers. Agilent provides this customary commercial license in Software and technical data pursuant to FAR 12.211 (Technical Data) and 12.212 (Computer Software) and, for the Department of Defense, DFARS 252.227-7015 (Technical Data - Commercial Items) and DFARS

227.7202-3 (Rights in Commercial Com-

puter Software or Computer Software Documentation).

Safety Notices

A CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.

A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met.

8000 Operator’s Manual

Figures 7

Tab le s 9

1Safety 11

Electrical Hazards 12

Warni ng 13 Caution 13 Note 13 Information Symbols 14

2 Introduction 15

8000 with Peristaltic Pump 17

Conventions Used in this Manual 17

Serial Number Format 18

3 Setting Up the 8000 19

Unpacking of 8000 and Components 20

Unpacking Your 8000 21 Unpacking / Setup of 8000 & Peristaltic Pump 22 Unpacking / Setup of 8000 & Syringe Pump 26 Unpacking / Setup of 8000, Syringe Pump, and Filter

Changer 29

Setup of 8000 and Components 34

Installing 8000 Full Flow Filters 34 Power and Network Connections 34 Installing the Sample Tray 36

8000 Operator’s Manual 3

Contents

Installing the Media Rinse Reservoir 36 Installing and Connecting the Autocalibration Block

(Peristaltic Pump only) 37 Installing the RM Module 38 Loading Filters (Filter Changer only) 40 Changing Filter Types (Filter Changer Only) 40

Hidden Key Functions 41

4 Operating the 8000 45

Operating the 8000 with Apparatus 1 / 2 46

8000 Keypad Options 46 8000 Menu Options - Apparatus 1 / 2 48 Creating a Sample Program 61 Starting a 8000 Program 66

Operating the 8000 with Apparatus 3 / 7 72

8000 Keypad Options 72 8000 with Apparatus 3 / 7 - Menu Options 74 Creating a Sample Program 86 Starting a Program 91

Manual Sample 95

Volumetric Calibration (Peristaltic Pump only) 97

Automatic Calibration 97 Manual Calibration 99 Checking the Calibration - Volume Accuracy 101

Daisy Chaining 102

Printer Operation and Communications 103

5 Maintenance and Troubleshooting 105

Preventive Maintenance 106

Clean System with Peristaltic Pump 106 Clean System Function with Syringe Pump 111

4 8000 Operator’s Manual

Syringe and Seal Replacement 112 Cleaning the Syringes 113 System Cleaning Procedure with Syringe Pump and Filter

Changer (optional) 115 Cleaning the Filter Changer Drip Tray 117 Cleaning the Media Replacement Pump 117

Report Center Impact Printer 119

Installing the Cartridge Ribbon 119 Replacing a Paper Roll 120 Toggling Your Printer Online 121 Printer Self Test 121 Printer Configuration 122 Obtaining Warranty and Other Services 123

Index 125

Contents

8000 Operator’s Manual 5

Contents

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6 8000 Operator’s Manual

Figures

Figure 1. Agilent 8000 Dissolution Sampling Station 21 Figure 2. Peristaltic Pump 22 Figure 3. Cable Connections for 8000, Peristaltic Pump, and VK

7000 / 7010 24 Figure 4. Resident Probe 25 Figure 5. 806 Syringe Pump 26 Figure 6. Cable Connections for 8000, Syringe Pump, and VK

7000 / 7010 27 Figure 7. Y Cable 5075-0446 and Adapter 5075-0852 28 Figure 8. 808 Filter Changer 30 Figure 9. Cable Connections for 8000, Syringe Pump, Filter

Changer, and VK 7000 / 7010 31 Figure 10. Y Cable 5075-0446 and Adapter 5075-0852 32 Figure 11. Resident Probe Housing and Sample Cannula 33 Figure 12. Internal Media Replacement Module 38 Figure 13. External Media Replacement Module 39 Figure 14. 8000 Keypad 46 Figure 15. 8000 Keypad 72 Figure 16. The Cleaning Process 108

8000 Operator’s Manual 7

Figures

This page was intentionally left blank, except for this message.

8 8000 Operator’s Manual

Tables

Table 1. Hidden Key Functions 41 Table 2. Front Panel Options (Apparatus 1 / 2) 47 Table 3. Main Menu Options (Apparatus 1 / 2) 49 Table 4. Set Clock and Alarms Options (Apparatus 1 / 2) 51 Table 5. Disso Parameters Options (Apparatus 1 / 2) 53 Table 6. Set Syringe Pump Parameters (Apparatus 1 / 2) 55 Table 7. Disso Control/Monitor Options (Apparatus 1 / 2) 57 Table 8. Manual Operations Options (Apparatus 1 / 2) 58 Table 9. Operate Syringe/Filter Changer Options (Apparatus 1 /

2) 59 Table 10. Program Variables Options (Apparatus 1 / 2) 62 Table 11. Select Start Mode Options (Apparatus 1 / 2) 68 Table 12. Disso Options (Apparatus 1 / 2) 69 Table 13. Select Disso Program Start Mode Options (Apparatus

1 / 2) 71 Table 14. Front Panel Options (Apparatus 3 / 7) 73 Table 15. Main Menu Options (Apparatus 3 / 7) 75 Table 16. Set Clock and Alarms Options 77 Table 17. Set App 3/7 Parameters Options (Apparatus 3 /

7) 79

Table 18. Set Syringe Pump Parameters Options (Apparatus 3 /

7) 81 Table 19. App 3/7 Control/Monitor Options 83 Table 20. Manual Operations (Apparatus 3 / 7) 84 Table 21. Operate Syringe/Filter Changer Options 85 Table 22. Program Variables Options (Apparatus 3 / 7) 88 Table 23. Select Start Mode Options (Apparatus 3 / 7) 93 Table 24. App 3/7 Options 94

8000 Operator’s Manual 9

Tables

Table 25. Manual Sampling Options 95 Table 26. Volume Accuracy Parameter Options 101 Table 27. Print Selections Options 104 Table 28. Program Auto Clean Variables 110 Table 29. Disso Parameters. 115 Table 30. Program Variables 116

10 8000 Operator’s Manual

Agilent 8000 Dissolution Sampling Station Operator’s Manual

1 Safety

Electrical Hazards 12

The Agilent 8000 has been designed and tested so that when used properly you have an accurate, fast, flexible, and safe instrument.

If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Operation of a Agilent 8000 involves the use of aqueous liquids and various pharmaceutical dosage forms. Unskilled, improper, or careless use of this instrument can create shock hazards, fire hazards, or other hazards which can cause death, serious injury to personnel, or severe damage to equipment and property.

Information on safety practices is provided with your instrument and operation manuals. Before using your instrument or accessories, you must thoroughly read these safety practices.

Observe all relevant safety practices at all times.

Agilent Technologies

1Safety

Electrical Hazards

The 8000 contains electrical circuits, devices, and components operating at dangerous voltages. Contact with these circuits, devices, and components can cause death, serious injury, or painful electric shock.

Panels or covers that are retained by fasteners which require the use of a tool for removal may be opened only by Agilent-trained, Agilent-qualified, or Agilent-authorized service engineers. Consult the manuals or product labels supplied with the 8020 to determine which parts are operator-accessible.

Application of the wrong supply voltage, connection of the instrument to an incorrectly wired supply outlet, or lack of proper electrical grounding can create a fire hazard or a potentially serious shock hazard and could seriously damage the instrument and any attached ancillary equipment.

Always use a three-wire outlet with ground connection which is adequately rated for the load. The installation must comply with local, state, and federal safety regulations.

Do not connect the instrument to the main power supply until you have made sure that the operating voltage is correctly set for the main power supply in the specific outlet in your laboratory to which the equipment will be connected.

12 8000 Operator’s Manual

Warning

WARNING

CAUTION

NOTE

Caution

Safety 1

A ‘Warning’ message appears in the manual when failure to observe instructions or precautions could result in death or injury.

Read all warnings and cautions carefully and observe them at all times.

A ‘Caution’ message appears in the manual when failure to observe instructions could result in damage to equipment (Agilent supplied and / or other associated equipment).

8000 Operator’s Manual 13

Note

A ‘Note’ appears in the manual to give advice or information.

1Safety

Information Symbols

Switches main power on

Switches main power off

Indicates single-phase alternating current

Indicates the product complies with the requirements of one or more European Union (EU) directives.

Indicates specific equipment meets standards of safety. These products are safe for use in the workplace for North America.

Indicates that this product must not be disposed of as unsorted municipal waste.

All Agilent products that are subject to the WEEE directive shipped after August 13, 2005 are compliant with the WEEE marking requirements. Such products are marked with the “crossed out wheelie bin” WEEE symbol in accordance with European Standard EN 50419.

For more information on collection, reuse, and recycling systems, please contact your local/regional waste administration, your local distributor, or Agilent.

Indicates the product complies with regulatory compliance requirements of New Zealand and Australia.

14 8000 Operator’s Manual

Agilent 8000 Dissolution Sampling Station Operator’s Manual

2 Introduction

8000 with Peristaltic Pump 17

Conventions Used in this Manual 17

Serial Number Format 18

Agilent Technologies

2 Introduction

The 8000 Dissolution Sampling Station provides an unattended solution for automated sampling from a dissolution apparatus. The instrument may be configured to control any instrument setup with a USP apparatus 1 (Baskets), 2 (Paddles), 3 (Reciprocating Cylinder), 5 (Paddle over Disk), 6 (Rotating Cylinder), or 7 (Reciprocating Holder) offered by Agilent Technologies, Inc. The firmware can store up to 15 different sampling programs. Dissolution apparatus parameters are entered as part of the 8000 program. These parameters are downloaded to the dissolution apparatus when beginning a test with the 8000.

The 8000 fail-safe sample delivery uses either the 810 Peristaltic Pump or 806 Syringe Pump optionally coupled with the automated 808 Filter Changer. Hard-copy documentation is provided by the built-in Report Center Printer which prints all essential parameters for proof of proper operation and compliance with current GMPs.

The 8000 may be configured with a replacement media (RM) module which replenishes any sample volume removed with fresh media. Sample is delivered into test tubes or pre-capped HPLC autosampler vials for direct transfer of samples to an HPLC system. Sample trays are available in a variety of sizes to accommodate various sizes of vials or test tubes.

The 8000 is automation ready. An RS232 port permits integration with other automated systems. Sampling runs can be activated with an external switch closure or TTL signal. The 8000 has a programmable TTL output to control the instruments in the system.

16 8000 Operator’s Manual

8000 with Peristaltic Pump

The 8000, when used in conjunction with the peristaltic pump, is capable of performing unattended sampling from a dissolution apparatus. All parameters of operation are controlled by the 8000. Sampling is carried out automatically and takes into account all relevant variables in the sample flow path such as tubing length, tension, and diameter. The volume calibration may be performed manually or automatically using an autocalibration block supplied with the unit. Samples may be delivered into open test tubes or pre-capped autosampler vials.

Conventions Used in this Manual

• Items you are asked to press are in bold. For example, “press H on

the keypad”.

• Key sequences you are asked to press appear like this: MENU > 7.

Introduction 2

8000 Operator’s Manual 17

2 Introduction

Serial Number Format

Syntax Code Meaning Description

CC Country of origin 2 alpha characters matching the required trade designation for

12 Year of manufacture ‘09’ for 2009, ‘10’ for 2010, etc.

34 Week of manufacture ‘01’ for week 1 to ‘52’ for week 52

The serial number contains 10 characters and follows this syntax:

CC1234xxxx

the country of origin

18 8000 Operator’s Manual

Agilent 8000 Dissolution Sampling Station Operator’s Manual

3 Setting Up the 8000

Unpacking of 8000 and Components 20

Setup of 8000 and Components 34

Hidden Key Functions 41

Agilent Technologies

3 Setting Up the 8000

WARNING

Unpacking of 8000 and Components

Check the contents for any damages due to shipping. The packaging used to ship your equipment ensures that damage rarely occurs, but if it does, contact both the carrier who delivered the instrument and the Dissolution Systems Service Department. Though claims for damage should be filed with the carrier, we will be glad to help you file a claim and get you up and running as quickly as possible.

For all configurations, ensure the equipment is configured for the voltage supplied.

The electrical connection at the back of the 8000 is the primary disconnect for the instrument. The instrument should be positioned to allow accessibility to the power cords for easy disconnection.

20 8000 Operator’s Manual

Unpacking Your 8000

WARNING

Figure 1 Agilent 8000 Dissolution Sampling Station

Setting Up the 8000 3

8000 Operator’s Manual 21

The 8000 is shipped in foam-filled cartons. The number of cartons may vary depending on your configuration.

1 Carefully remove the 8000 and accessory kit(s) from their shipping

cartons. Remove all cushioning material and tape.

2 Place the 8000 on a clear, dry, level section of the bench top close to

the dissolution apparatus. The preferred placement of the 8000 is on the right side of the dissolution apparatus.

Unscrew and remove the two clamps which secured the 8000 dispensing arm during shipping. These clamps are located on both sides of the right dispensing support arm where it connects to the 8000 main body. Failure to remove the two clamps prior to powering up the 8000 may damage the unit.

3 Setting Up the 8000

Unpacking / Setup of 8000 & Peristaltic Pump

Environmental Requirements for Peristaltic Pump Installation

• Altitude: max 2000m;

• Humidity: max relative humidity 80% for temperatures up to 31 ºC

decreasing linearly to 50% relative humidity at 40 ºC

• Indoor use only

• Temperature: 5 ºC to 40 ºC

• Main supply voltage fluctuations are not to exceed ± 10% of the

nominal supply voltage

• Operating conditions: 3 mins ON Max; 5 mins OFF Min

Figure 2 Peristaltic Pump

Installation

1 Carefully remove the peristaltic pump from its shipping carton.

Remove all cushioning and tape.

2 Place the peristaltic pump on a clear, dry, level section of the bench

top between the 8000 and the dissolution apparatus.

3 Remove the two screws on top of the pump. Lift off the plastic cover

and set it aside.

4 Remove the cartridges from the pump by pressing down the levers on

the right side. Lift the cartridges up and away from the pump.

22 8000 Operator’s Manual

Setting Up the 8000 3

NOTE

WARNING

5 Loosely fit the peristaltic pump tubing along the inside groove of the

first cartridge, locking the tubing clips on the outside notches.

Ensure that all pump cartridges are installed. It is not necessary to install tubing on any unused positions.

6 Refit the cartridge to the pump by hooking the clip on the bottom left

of the cartridge into the groove on the left side of the pump.

7 Push down the right side of the cartridge, ensuring that the tubing

remains under the cartridge, and pull up the lever to lock the cartridge in place. If desired, trim the excess tubing to within one inch of the tubing clips.

The cover does not fit unless the cartridges are positioned with the levers on the right side.

8 Repeat Steps 5 - 7 for each remaining cartridge.

9 Refit the cover and replace the two screws. If the cover is not

replaced correctly, the pump will not operate.

8000 Operator’s Manual 23

The electrical connection at the back of the 8000 is the primary disconnect for the instrument. The instrument should be positioned to allow accessibility to the power cords for easy disconnection.

10 Refer to the following example diagram to correctly make cable

connections for the 8000, peristaltic pump, and dissolution

3 Setting Up the 8000

apparatus:

Figure 3 Cable Connections for 8000, Peristaltic Pump, and VK

7000 / 7010

11 Connect the five-pin cable (#2, P/N 5075-0033) between the jack on

the 8000 rear panel labeled PUMP and the jack on the peristaltic pump rear panel labeled PUMP CONTROL.

12 If the system configuration includes the 705-DS, connect the 9-to-25

pin cable to the RS232 port on the dissolution apparatus and the RS232 COMPUTER port on the 8000 rear panel.

Otherwise, connect the RS232 cable (#1, P/N 5075-0042) between the RS232 port on the dissolution apparatus and the RS232 COMPUTER port on the 8000 rear panel.

24 8000 Operator’s Manual

Setting Up the 8000 3

NOTE

sample cannula

resident probe housing

13 The 8000 is shipped with all sample tubing attached to the valves

inside the dispensing arm.

Do not remove the nylon tie wraps securing the tubing into bundles.

The direction of the peristaltic pump can be set via a switch on its rear panel. It does not matter at this point to which side the bundles go, as long as all lines in the bundle from the sampling cannulas are connected to one side, and all lines in the bundle from the valve inlets to the other.

14 If your system configuration includes the 705-DS, connect the

resident probes (see Figure below) to the tubing and attach the lines to the tubing on one side of the appropriate peristaltic pump cartridges.

Otherwise, connect the tubing from the dissolution apparatus sample cannulas to the tubing on one side of the appropriate peristaltic pump cartridges.

8000 Operator’s Manual 25

Figure 4 Resident Probe

15 Connect another length of tubing between the remaining side of the

peristaltic pump cartridges and the tubing coming from the left positions of the 8000 solenoid valves. Ensure each cartridge has identical color tubing connected at both the inlet and the outlet.

3 Setting Up the 8000

WARNING

Unpacking / Setup of 8000 & Syringe Pump

16 Connect the remaining length of tubing coming from the top

positions of the 8000 solenoid valves to the return lines on the dissolution apparatus. Ensure each line is attached to its identical color.

Do not tighten the plastic fittings on the ends of the tubing more than half a turn past finger-tight. Overtightening will damage the fittings.

17 For a system that includes a 705-DS, connect the return lines to the

return cannulas of the resident probes.

26 8000 Operator’s Manual

Figure 5 806 Syringe Pump

The syringe pump is shipped in individual packaging.

1 Carefully lift the unit out of the box.

2 Remove any cushioning material.

Setting Up the 8000 3

3 Place the 806 Syringe Pump on a clean, dry, level section of the

bench top between the 8000 and the dissolution apparatus.

4 Refer to the following example diagram to correctly make cable

connections for the 8000, syringe pump, and dissolution apparatus:

Figure 6 Cable Connections for 8000, Syringe Pump, and VK 7000 /

7010

5 Connect the single end of the Y cable (#1, P/N 5075-0446) to the

RS232 port on the syringe pump.

6 Connect an RS232 cable (#2, P/N 5075-0252) between the RS232

COMPUTER port on the 8000 and one extension of the Y cable attached to the syringe pump.

7 If the system configuration includes the 705-DS, connect a

9-to-9-pin cable (P/N 5075-0448) from the RS232 port on the dissolution apparatus rear panel to the remaining extension of the Y cable attached to the syringe pump.

Otherwise, connect a second RS232 cable (#2, P/N 5075-0252) between the remaining extension of the Y cable attached to the syringe pump and the RS232 port on the dissolution apparatus.

8000 Operator’s Manual 27

3 Setting Up the 8000

NOTE

8 A system configuration that includes a non-Agilent dissolution

apparatus, requires use of an adapter (P/N 5075-0852, see following Figure). This adapter should be attached to the #2 port of the Y-cable in place of the cable connected to the dissolution apparatus.

Figure 7 Y Cable 5075-0446 and Adapter 5075-0852

28 8000 Operator’s Manual

9 The 8000 is shipped with all sample tubing attached to the valves

inside the dispensing arm.

Do not remove the nylon tie wraps securing the tubing into bundles.

10 If the system configuration includes the 705-DS, connect the resident

probes to the sample tubing and attach the lines to the left port (labeled IN) of each syringe starting with Position 1.

Otherwise, connect the tubing from the dissolution apparatus sampling cannulas to the left port (labeled IN) of each syringe starting with Position 1.

11 Connect a separate length of tubing between the right port (labeled

OUT) of each syringe to the tubing coming from the left positions of

Setting Up the 8000 3

WARNING

the 8000 solenoid valves. Ensure each line is attached to its identical color.

12 Connect the remaining length of tubing from the top positions of the

8000 solenoid valves to the return lines on the dissolution apparatus. Ensure each line is attached to its identical color.

Do not tighten the plastic fittings on the ends of the tubing more than half a turn past finger-tight. Overtightening will damage the fittings.

13 If the system configuration includes the 705-DS, connect the return

lines to the return cannulas of the resident probes.

Unpacking / Setup of 8000, Syringe Pump, and Filter Changer

Environmental Requirements for Filter Changer Installation

• Altitude max.2000m;

• Humidity max relative humidity 80% for temperatures up to 31 ºC

decreasing linearly to 50% relative humidity at 40ºC;

• Indoor use only

• Temperature: 5 ºC to 40 ºC;

• Mains supply voltage fluctuations are not to exceed ± 10% of the

nominal supply voltage.

• Operating conditions 8 mins ON Max; 5 mins OFF min

8000 Operator’s Manual 29

3 Setting Up the 8000

Installation

Figure 8 808 Filter Changer

30 8000 Operator’s Manual

The filter changer is shipped in individual packaging.

1 Carefully lift the unit out of the box.

2 Remove any cushioning material.

3 Place the unit on a clear, dry, level section of the bench top between

the 8000 and the syringe pump.

Setting Up the 8000 3

4 Refer to the following example diagram to correctly make cable

connections for the 8000, syringe pump, filter changer, and dissolution apparatus:

Figure 9 Cable Connections for 8000, Syringe Pump, Filter Chang-

er, and VK 7000 / 7010

5 Connect the single end of a Y cable (#1, P/N 5075-0446) to the

RS232 port on the filter changer.

6 Connect an RS232 cable (#2, P/N 5075-0252) between the RS232

COMPUTER port on the 8000 and one extension of the Y cable attached to the filter changer.

7 Connect a 9-9 pin RS232 cable (#3, P/N 5075-0449) from the

remaining extension of the Y cable attached to the filter changer to one extension of a separate Y-cable.

8 Connect the single end of the second Y cable (#1, P/N 5075-0446) to

the RS232 port on the syringe pump.

9 If the system configuration includes the 705-DS, connect a

9-to-9-pin cable (P/N 5075-0448) from the RS232 port on the dissolution apparatus rear panel to the remaining extension of the Y cable attached to the syringe pump.

Otherwise, connect a second RS232 cable (#2, P/N 5075-0252)

8000 Operator’s Manual 31

3 Setting Up the 8000

NOTE

between the remaining extension of the Y cable attached to the syringe pump and the RS232 port on the dissolution apparatus.

10 A system configuration that includes a non-Agilent dissolution

32 8000 Operator’s Manual

Figure 10 Y Cable 5075-0446 and Adapter 5075-0852

11 The 8000 is shipped with all sample tubing attached to the valves

inside the dispensing arm.

Do not remove the nylon tie wraps securing the tubing into bundles.

12 If the system configuration includes the 705-DS, connect the resident

probes to the sample tubing and attach the lines to the left port (labeled IN) of each syringe starting with Position 1.

Otherwise, connect the tubing from the dissolution apparatus sampling cannulas to the left port (labeled IN) of each syringe

Setting Up the 8000 3

sample cannula

resident probe housing

WARNING

starting with Position 1.

Figure 11 Resident Probe Housing and Sample Cannula

13 Connect a separate length of tubing from the right port (labeled

OUT) of each syringe to the top inlet ports of the filter changer starting with the port closest to the carousel.

14 Connect another separate length of tubing lines from the lower side

ports of the filter changer to the tubing coming from the left positions of the 8000 solenoid valves. Ensure each line is attached to its identical color.

15 Connect the tubing coming from the top positions of the 8000

solenoid valves to the return lines of the dissolution apparatus. Ensure each line is attached to its identical color.

8000 Operator’s Manual 33

Do not tighten the plastic fittings on the ends of the tubing more than half a turn past finger-tight. Overtightening will damage the fittings.

16 If the system configuration includes the 705-DS, connect the return

lines to the return cannulas of the resident probes.

17 To install the catch basin for the filters, slide the side of the basin

containing the opening over the screw mounted on the front of the filter changer.

3 Setting Up the 8000

WARNING

Setup of 8000 and Components

Installing 8000 Full Flow Filters

The 8000 has been supplied with 10-, 35-, and 70-micron Full Flow Filters for use with the sampling cannulas. In theory, dissolution continues until the samples are filtered. The 8000 allows filtering at the instant a sample is taken with the use of the Full Flow Filters. Some drug excipients may clog the Full Flow filters reducing their performance. Filters may need to be changed during a run only in extreme cases.

Install Full Flow Filters on the sampling cannulas to prevent clogging of the sampling lines. Damage to your equipment will result if the sampling lines clog. For best results, use the 10-micron Full Flow Filter.

Full Flow Filters are not for use with Apparatus 3 / 7 configurations.

34 8000 Operator’s Manual

Power and Network Connections

The electrical connection at the back of the 8000 is the primary disconnect for the instrument. The instrument should be positioned to allow accessibility to the power cords for easy disconnection.

Ensure the 8000 and any coupled components are configured for the voltage supplied at the receptacle.

• If your system configuration includes the peristaltic pump, the power

receptacle, and power switch are located on the rear of the unit. Connect the power cord and turn on the peristaltic pump.

Setting Up the 8000 3

• If your system configuration includes the syringe pump, the power

receptacle, and power switch are located on the left side of the unit. Connect the power cord and turn on the syringe pump.

• If your system configuration includes the filter changer, the power

receptacle, and power switch are located on the rear of the unit. Connect the power cord and turn on the filter changer.

The 8000 has a dual voltage (115 V / 230 V) option on the power entry module. Ensure the voltage indicated on the fuse drawer matches the power outlet. Connect the power cord to the rear of the 8000. Turn on the 8000. The system monitor illuminates. The system monitor displays the initial status screen and then changes to the Ready screen.

8000 FRACTION COLLECTOR

PROGRAM REVISION x.xx

INITIALIZING....PLEASE WAIT

TIME: 01/01/12 21:56:40 ELAPSE: 000:00:00

The firmware version (program revision) displays. This is the only time the firmware version displays. Record the number below and refer to it if you need to call the Dissolution Systems Service Department.

Firmware version

While the unit is powering up, the dispensing arm returns to its home position at the rear of the unit and the firmware program initializes. If the dispensing arm is already at the home position when the unit is powered up, the initial status screen displays for three seconds only. When the initial status screen disappears, the Ready screen displays. From the Ready screen, you can access the Main Menu or any of the manual functions.

READY

PRG# 01 R-M: ENABLED VOLUME: 5 mL

TIME: 01/01/12 21:56:40 ELAPSE: 000:00:00

8000 Operator’s Manual 35

3 Setting Up the 8000

WARNING

NOTE

Installing the Sample Tray

Installing the Media Rinse Reservoir

Place the supplied sample tray on top of the unit. The sample tray has four grommeted corner holes which fit over the four locating pins.

The sample tray must be securely mounted on the locating pins. Otherwise, damage can occur to the sampling needles and drive mechanism.

The media rinse reservoir fits in the rear of the unit between the metal brackets. The dispensing arm must be moved forward to put it in place.

1 From the Ready screen, press MENU.

2 Select option 7, Manual Operation.

3 Select option 4, Goto Rows.

4 Enter 4 to move the dispensing arm to Row 4. The dispensing arm

moves forward to allow access to the rear of the 8000.

5 Place the media rinse reservoir between the metal brackets directly

behind the sample tray.

36 8000 Operator’s Manual

The media rinse reservoir has two chambers—one for replacement media and one for rinsing. The replacement media chamber has two barbed fittings—one on each end of the tank. The rinse chamber has a single barbed fitting. Orient the reservoir with the rinse chamber toward the front.

6 Connect the six-foot length of clear Tygon tubing to the barbed

elbow fitting located on the rinse chamber of the reservoir. Place the other end in a sink, drain, or suitable waste container.

7 If your system configuration includes the syringe pump, press H to

move the dispensing arm to the home position and press ESC three times to return to the Ready screen.

Setting Up the 8000 3

If your system configuration includes the peristaltic pump, complete the steps under “Installing and Connecting the Autocalibration Block

(Peristaltic Pump only)” on page 37.

Installing and Connecting the Autocalibration Block (Peristaltic Pump only)

1 With the dispensing arm forward, insert the autocalibration block

behind the media rinse reservoir. Ensure the RS232 port is positioned to the right for a 6-row configuration and toward the back for a 12-row configuration.

2 Connect the nine-pin RS232 cable between the CALIBRATION port

on the 8000 rear panel and the RS232 port on the side of the autocalibration block.

3 Press H to move the dispensing arm to the home position.

4 Press ESC three times to return to the Ready screen.

8000 Operator’s Manual 37

3 Setting Up the 8000

Pump 1

Pump 2

Installing the RM Module

Internal Unit

Figure 12 Internal Media Replacement Module

1 Attach a length of 1/2-inch diameter tubing to the IN fitting of pump

1 (the top pump).

2 Place the free end in one of the unstirred vessels containing medium

or in the receptacle being used to hold the replacement medium.

3 If desired, attach a clip to regulate flow speed on this length of

tubing.

4 Connect a length of tubing from the OUT fitting of pump 1 to the IN

fitting on the RM chamber of the media rinse reservoir.

5 Attach a length of tubing between the OUT fitting of the RM

chamber and the IN fitting on pump 2 (the bottom pump).

6 Attach a length of tubing to the OUT fitting on pump 2 and place the

free end in the replacement medium vessel or receptacle.

38 8000 Operator’s Manual

Setting Up the 8000 3

External Unit

Figure 13 External Media Replacement Module

1 Remove the cover housing the RM chamber from the side of the

8000.

2 Detach the RM chamber.

3 Insert the end of the tubing attached to the IN fitting on the pump

into the plastic RM chamber.

4 Slide a tubing clamp on a length of tubing.

5 Connect one end of the tubing containing the clamp to the OUT

fitting on the pump. Connect the other end to the IN fitting of the RM chamber.

6 Connect a length of plastic tubing to the OUT fitting of the RM

chamber.

7 Place the free end of this piece in the plastic RM chamber.

8 Reattach the RM chamber, tighten all screws, and replace all covers.

8000 Operator’s Manual 39

3 Setting Up the 8000

WARNING

Loading Filters (Filter Changer only)

1 Choose a tube of filters. Although you can load up to eight tubes of

filters, only one tube is necessary for the filter changer to function properly.

2 Remove the cap from the end of the tube to expose the long, thin exit

nozzle of the end filter.

3 Insert the tube, with the open end down, into the metal filter

assembly in the filter changer carousel. Pivot the tube until it slides vertically into the white stabilizer slot.

4 Repeat Steps 2 - 4 to load additional tubes of filters.

Use only Agilent-certified filters or Millipore-equivalent types of filters. Other types of filters may not work properly in the filter changer and could damage the unit.

40 8000 Operator’s Manual

Changing Filter Types (Filter Changer Only)

1 Remove all tubes of filters from the carousel of the filter changer.

2 From the 8000 Ready screen, press MENU. The Main Menu

displays.

3 Select option 7, Manual Operation. The Manual Operations screen

displays.

4 Select option 6, Syringe/Filter.

5 From the Operate Syringe/Filter Changer screen, select option 5,

Filter Changer. The Filter Changer Manual Control screen displays.

6 Select option 3, Change Filters.

7 Enter 8 as the number of filters to change and press ENTER. The

filters expel from the filter changer.

8 Repeat Steps 6 and 7 until filters no longer expel from the filter

changer. The following error message displays on the screen:

CHANGER ROTOR ERROR. THE FILTER CHANGER IS EMPTY OR HAS FAILED TO LOAD PROPERLY. PRESS <1> TO CONTINUE OR <ESC> TO QUIT.

9 Press ESC. The Filter Changer Manual Control screen displays.

FILTER

10 Load the new tubes of filters into the carousel of the filter changer.

11 Select option 3, Change Filters.

12 Enter 8 as the number of filters to change and press ENTER. The

13 When the filter changer stops expelling filters, select option 3,

14 Enter 1 as the number of filters to change and press ENTER. The

15 Press ESC four times to return to the Ready screen.

Hidden Key Functions

Tabl e 1 Hidden Key Functions

Setting Up the 8000 3

See “Loading Filters (Filter Changer only)” on page 40.

filters expel from the filter changer.

Change Filters, again.

remaining filter of the previous type expels from the filter changer and is replaced with the new filter.

Key Sequence Function

MENU > 0 > 0 Use this key sequence to select the system configuration. Select the option that

corresponds to the number of valves on your 8000.

MENU > 0 > 8 Use this key sequence to select a fixed 1200 baud or a programmable baud. Select

option 2, Programmable Baud.

Note: If you are using the 8000 with other Agilent equipment, the baud rate must be set to 9600 baud (see “Printer Operation and Communications” on page 103).

MENU > 0 > A Use this key sequence to set the drop volume before sampling. Enter the desired drop

volume and press ENTER. The maximum acceptable value is 1.

Note: The drop volume is an amount of sample that is dispensed through the 8000 needles prior to dispensing samples into the collection tubes to ensure the needles are purged completely.

MENU > 0 > B Use this key sequence to enable or disable the moving alert. The asterisk (*) displays

next to the current selection. If enabled, the alarm sounds when the dispensing arm is in motion.

MENU > 0 > C Use this key sequence to enable or disable the delayed start alarm. The asterisk (*)

displays next to the current selection. If the delayed start alarm is enabled, the alarm sounds prior to the start of the program. See “DISSO Options Screen” on page 69.

8000 Operator’s Manual 41

3 Setting Up the 8000

Tabl e 1 Hidden Key Functions

Key Sequence Function

MENU > 0 > D Use this key sequence to enable or disable the multiple sample option. The asterisk (*)

MENU > 0 > E Use this key sequence to select the printer type. Ensure the asterisk (*) displays next

MENU > 0 > F Use this key sequence to select the date display style. The asterisk (*) displays next to

MENU > 0 > P Use this key sequence to enable or disable the syringe pump. The asterisk (*) displays

MENU > 0 > R Use this key sequence to enable or disable media replacement for the syringe pump.

displays next to the current selection.

Note: For use with APP 3/7 (Apparatus 3 / 7) systems only.

to option 1, Impact. If not, select option 1 to enable the impact printer.

Note: Select option 1, Impact, or the instrument will not operate properly.

the current selection. Select option 1, MM/DD/YY, or option 2, DD/MM/YY.

next to the current selection.

If your system configuration includes the syringe pump, you must enable the syringe pump control options on the 8000 prior to setting the syringe pump parameters (see “3

SET SYRINGE PUMP” on page 48.

The asterisk (*) displays next to the current selection. Select option 1, Enable, or option 2, Disable, as appropriate.

Note: This option is not available for APP 3/7 (Apparatus 3 / 7) systems.

42 8000 Operator’s Manual

Setting Up the 8000 3

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8000 Operator’s Manual 43

3 Setting Up the 8000

44 8000 Operator’s Manual

Agilent 8000 Dissolution Sampling Station Operator’s Manual

4 Operating the 8000

Operating the 8000 with Apparatus 1 / 2 46

Operating the 8000 with Apparatus 3 / 7 72

Manual Sample 95

Volumetric Calibration (Peristaltic Pump only) 97

Daisy Chaining 102

Printer Operation and Communications 103

Agilent Technologies

4 Operating the 8000

Operating the 8000 with Apparatus 1 / 2

8000 Keypad Options

The keypad on the 8000 is similar to a personal computer keyboard. For example, it has a SPACE key, an ENTER key, and a SHIFT key. These keys function exactly like their counterparts on a personal computer or typewriter keyboard. See Figure 14, “8000 Keypad,” below. A discussion about each of the main function keys follows.

Figure 14 8000 Keypad

46 8000 Operator’s Manual

Operating the 8000 4

The front panel options include the following:

Tabl e 2 Front Panel Options (Apparatus 1 / 2)

Option Function

ESC Press ESC to stop execution of a running program or calibration routine. Press

ESC to move back one level to the previous menu or selection when moving

through the multi-level menu.

Note: Be very careful when using ESC while a program is running. If a program or calibration is running and ESC is pressed, a prompt appears on the first line of the display asking if you want to stop the running program. The options are Y or N. Press Y to stop the running program. Press N to continue the running program.

MENU Use this option to set the clock, alarms, DISSO parameters, start output delay /

syringe pump parameters, manual prime time / volume, manual purge time / volume as well as to remotely control the dissolution apparatus and manually control the 8000.

PRINT Press PRINT to record the batch information, print the sample times, enable or

disable the Report Center Printer, select a local or remote printer, set the communication port identification number, and set the baud rate.

CAL Use this option to calibrate the 8000 to ensure that sample volumes are

accurately and precisely taken.

Note: The CAL key on the 8000 can be used only if the peristaltic pump is installed on your system.

PROG Use this option to set program parameters.

START PROG Use this option to start a program.

MANUAL SAMPLE Use this option to collect a sample from the dissolution vessels on command

without a program.

OPEN VALVES Press OPEN VALVES to open all valves simultaneously. This function keeps

the valves open as long as the key on the keypad is pressed. To change the valve configuration, press MENU > 0 > 0 and select the appropriate configuration.

Press (PROBES UP / DOWN) to raise or lower the sampling cannulas on

(PROBES UP / DOWN)

the dissolution apparatus.

Note: Not available with APP 3/7 systems.

8000 Operator’s Manual 47

4 Operating the 8000

Tabl e 2 Front Panel Options (Apparatus 1 / 2)

Option Function

PUMP OFF

PUMP FWD

PUMP REV

CLEAN SYTEM Use this option to keep the valves, needles, and fluid lines clean.

LOCK KBRD Press LOCK KBRD to lock the keypad. Press LOCK KBRD again to unlock

PUMP OFF, PUMP FWD, and PUMP REV work exclusively with the Agilent peristaltic pump. When the syringe pump is connected, they are disabled.

the keypad.

8000 Menu Options - Apparatus 1 / 2

Main Menu

From the Ready screen, press MENU. The Main Menu displays. Options 3, 4, and 5 change depending on your system configuration.

When your system configuration includes the peristaltic pump, the following Main Menu displays:

***MAIN MENU*** 4 MANUAL PRIME TIME

1 SET CLOCK/ALARMS 5 MANUAL PURGE TIME

2 SET DISSO 6 CONTROL DISSO

3 START OUTPUT DELAY 7 MANUAL OPERATION

When your system configuration includes the syringe pump with or without the filter changer, the following Main Menu displays:

***MAIN MENU*** 4 MANUAL PRIME VOL.

1 SET CLOCK/ALARMS 5 MANUAL PURGE VOL.

2 SET DISSO 6 CONTROL DISSO

3 SET SYRINGE PUMP 7 MANUAL OPERATION

48 8000 Operator’s Manual

Following is a description of the Main Menu screen options:

Tabl e 3 Main Menu Options (Apparatus 1 / 2)

Option Function

1 Set Clock/Alarms Use this option to set the clock and alarms. If your system configuration includes

the syringe pump, the start output delay option also displays on this screen. See

“Main Menu Option 1 - Set Clock/Alarms” on page 51.

2 Set DISSO Use this option to set the DISSO parameters. See “Main Menu Option 2 - Set

DISSO” on page 53.

Operating the 8000 4

3 Start Output Delay

(peristaltic pump)

3 Set Syringe Pump (syringe pump)

4 Manual Prime Time (peristaltic pump)

If your system configuration includes the peristaltic pump, use this option to set the delay from the start of a program until the TTL signal is sent from the START OUTPUT jack on the 8000 rear panel.

Select option 3, Start Output Delay. Enter a delay length of up to 99:59 minutes for the 8000 TTL output and press ENTER. The Main Menu displays.

Note: This output signal can be used to start other instrumentation, such as a dissolution apparatus, after the program on the 8000 is started. The default delay is 00:00, indicating that the signal will be sent at the moment a program starts. For example, a value of 10:00 delays the signal for ten minutes after the program starts. Set the value to 00:00 to eliminate the delay feature.

When the syringe pump is enabled, Start Output Delay displays under the Set Clock and Alarms screen as option 6. See “6 Start Output Delay” on page 52.

If your system configuration includes the syringe pump, use this option to set the syringe pump parameters. See “Main Menu Option 3 - Set Syringe Pump” on page 55.

If your system configuration includes the peristaltic pump, use this option to set the interval the peristaltic pump runs forward, drawing medium into the system, before the valves open to deliver samples.

Select option 4, Manual Prime Time. Enter a value for the priming time from 1 to 99 seconds and press ENTER. The Main Menu displays.

Note: At a minimum, the manual prime time should be long enough for medium to be seen dripping from the return cannulas. In general, the longer the tubing lengths, the longer the priming time required. This value is not used by sampling programs, which have their own individual priming time values. It is used only with the MANUAL SAMPLE, CAL, and CLEAN SYSTEM keys on the 8000.

8000 Operator’s Manual 49

4 Operating the 8000

Tabl e 3 Main Menu Options (Apparatus 1 / 2)

Option Function

4 Manual Prime Vol.

(syringe pump)

5 Manual Purge Time (peristaltic pump)

5 Manual Purge Vol. (syringe pump)

If your system configuration includes the syringe pump, use this option to set the amount of drawn medium necessary to fill the sampling lines of the entire system.

Select option 4, Manual Prime Vol. Enter a value for the priming volume and press ENTER. The Main Menu displays.

Note: At a minimum, the manual prime volume should be large enough for medium to be seen dripping from the return cannulas. In general, the longer the tubing lengths, the larger the priming volume required. This value is not used by sampling programs which have their own individual priming volume values. It is used only with the MANUAL SAMPLE and CLEAN SYSTEM keys on the

8000.

If your system configuration includes the peristaltic pump, use this option to set the interval the peristaltic pump runs in reverse to return the uncollected medium in each line back into the vessel from which it was drawn.

Select option 5, Manual Purge Time. Enter a value for the manual purge time from 1 to 99 seconds and press ENTER. The Main Menu displays.

Note: Purging also clears the sampling filters of particulate matter that may restrict successive samples. As with priming, the longer the tubing lengths, the longer the purge time required. This value is not used by sampling programs, which have their own individual purging time values. It is used only with the MANUAL SAMPLE, CAL, and CLEAN SYSTEM keys on the 8000.

If your system configuration includes the syringe pump, use this option to set a purge volume that ensures all stranded medium is properly expelled.

Select option 5, Manual Purge Vol. Enter a value for the purge volume that ensures at least two plunger strokes and press ENTER. The Main Menu displays.

Note: The first stroke moves all medium from the sampling lines to the return lines. The second stroke draws air into the sampling lines and purges the remaining medium from the return lines. As with priming, the longer the tubing lengths, the larger the purge volume required. This value is not used by sampling programs which have their own individual purging volume values. It is used only with the MANUAL SAMPLE and CLEAN SYSTEM keys on the 8000.

6 Control DISSO Use this option to remotely control the dissolution apparatus from the 8000. See

“Main Menu Option 6 - Control DISSO” on page 57.

7 Manual Operation Use this option to manually control the 8000. See “Main Menu Option 7 -

Manual Operation” on page 57.

50 8000 Operator’s Manual

Operating the 8000 4

Main Menu Option 1 - Set Clock/Alarms

From the Main Menu, select option 1, Set Clock/Alarms. The Set Clock and Alarms screen displays.

***SET CLOCK AND ALARMS***

1 SET CLOCK 2 TIMER ALARM

3 ROW COMPL ALARM 4 CALIB. COMPL ALARM

5 PRG COMPL ALARM 6 START OUTPUT DELAY

Following is a description of the Set Clock and Alarms screen options:

Tabl e 4 Set Clock and Alarms Options (Apparatus 1 / 2)

Option Function

1 Set Clock Select option 1, Set Clock. Enter the date in the appropriate format and press

ENTER. The time displays in 24-hour format. If the time is correct, press ENTER or ESC to return to the Main Menu. If the time is incorrect, enter the

correct time in 24-hour format and press ENTER. The Set Clock and Alarms screen displays.

2 Timer Alarm Use this option to set the elapsed timer alarm. The elapsed timer alarm is a simple

timer you can use to time any interval from 1 minute to 99 hours. The alarm sounds when the time has expired. Once turned off, the timer does not operate again until you reset it.

Select option 2, Timer Alarm. Enter a duration in hh:mm format and press ENTER. The Set Clock and Alarms screen displays.

Press any key to silence the alarm after it sounds.

3 Row Compl Alarm Use this option to set an alarm to indicate the completion of each sample point.

This alarm is useful when the samples must be checked or removed for immediate analysis.

Select option 3, Row Compl Alarm. Select option 1 to enable or option 2 to disable the alarm. The Set Clock and Alarms screen displays.

Press any key to silence the alarm after it sounds.

8000 Operator’s Manual 51

4 Operating the 8000

Tabl e 4 Set Clock and Alarms Options (Apparatus 1 / 2)

Option Function

4 Calib. Compl Alarm Use this option to set an alarm to sound when autocalibration is complete and all

lines are purged.

Select option 4, Calib. Compl Alarm. Select option 1 to enable or option 2 to disable the alarm. The Set Clock and Alarms screen displays.

Press any key to silence the alarm after it sounds.

Note: This option is only available if your system configuration includes the peristaltic pump.

5 Prg Compl Alarm Use this option to set an alarm to sound at the end of the program. It is a useful

reminder that the program is complete.

Select option 5, Prg Compl Alarm. Select option 1 to enable or option 2 to disable the alarm. The Set Clock and Alarms screen displays.

Press any key to silence the alarm after it sounds.

6 Start Output Delay Note: Option 6 displays only when the syringe pump is enabled.

If the syringe pump is enabled, use this option to set the delay from the start of a program until the TTL signal is sent from the START OUTPUT jack on the 8000 rear panel.

Select option 6, Start Output Delay. The Start Output Delay screen displays. Enter a delay length of up to 99:59 minutes for the 8000 TTL output and press ENTER. The Set Clock and Alarms screen displays.

When the syringe pump is disabled, this option displays under the Main Menu.

See “3 Start Output Delay (peristaltic pump)” on page 49.

52 8000 Operator’s Manual

Operating the 8000 4

NOTE

Main Menu Option 2 - Set DISSO

From the Main Menu, select option 2, Set DISSO. Enter a program number and press ENTER. The DISSO Parameters screen displays.

***DISSO PARAMETERS*** 4 SET BATH TEMP (VESSEL TEMP)

1 SET SPINDLE RPM 5 COM PORT ID

2 FINAL SPIN LENGTH 6 DDM OR BASKET

3 INI/FINAL VESSEL TEMP 7 PRINT FREQ

DISSO applies to all dissolution apparatus.

Following is a description of the DISSO Parameters screen options:

Tabl e 5 Disso Parameters Options (Apparatus 1 / 2)

Option Function

1 Set Spindle RPM Select option 1, Set Spindle RPM. The Parameter for Manual or Program screen

displays.Select option 1, Manual, to set an RPM to be used as the default for manual operation (see “Main Menu Option 6 - Control DISSO” on page 57). Optionally, select option 2, Program, to set an RPM to be used as a default for programmed operation (see “Starting a 8000 Program” on page 66). Enter the desired RPM and press ENTER. The DISSO Parameters screen displays.

8000 Operator’s Manual 53

2 Final Spin Length Select option 2, Final Spin Length, to program a short, high-speed spin cycle at

250 RPM at the end of the test. Enter a duration for the final spin and press ENTER. The DISSO Parameters screen displays.

Note: Many dissolution protocols now call for an infinity sample to be taken after the end of the test.

3 Ini/Final Vessel Temp Select option 3, Ini/Final Vessel Temp. The Initial Vessel Temp Measurement screen

displays. Select option 1 to enable or option 2 to disable the initial vessel temperature reading. The Final Vessel Temp Measurement screen displays. Select option 1 to enable or option 2 to disable the final vessel temperature reading. The DISSO Parameters screen displays.

4 Operating the 8000

Tabl e 5 Disso Parameters Options (Apparatus 1 / 2)

Option Function

4 Set Bath Temp

4 Vessel Temp

5 Com Port ID Select option 5, Com Port ID, to change the communication port identification

6 DDM or Basket Select option 6, DDM or Basket, to select the apparatus type. The Select Basket or

If your system configuration includes a dissolution apparatus with a water bath, select option 4, Set Bath Temp. If your system configuration includes a dissolution apparatus with Direct Vessel Heating (DVH), select option 4, Vessel Temp.

Select option 1, Manual, to set the water bath / vessel temperature. Optionally, select option 2, Program, to set a desired water bath / vessel temperature to download to the dissolution apparatus. Enter the desired temperature and press ENTER. The DISSO Parameters screen displays.

number. Your system was shipped with the communication port identification number set to 01. You can set the identification number to any number between 1 and 99. The identification number is used when multiple units are connected in series so that the controlling or master 8000 can start individual units.

Enter the communication port identification number and press ENTER. The DISSO Parameters screen displays.

Paddle of DISSO screen displays.

If using baskets, select option 1, Basket. The DISSO Parameters screen displays.

If using paddles and sequential delivery, select option 2, Paddle. The Dosage Delivery Mode of DISSO screen displays. Select option 1, Sequential, enter a time interval for the dosage drop and press ENTER. The DISSO Parameters screen displays.

Note: Only use the sequential delivery option if your dissolution apparatus is equipped with clutches.

If using paddles and simultaneous delivery, select option 2, Paddle. The Dosage Delivery Mode of DISSO screen displays. Select option 2, Simultaneous. The DISSO Parameters screen displays.

7 Print Freq The dissolution apparatus status prints at each sample time point. Select option 7,

Print Freq, to set additional times to print the dissolution apparatus status. This option should be reserved for use when there is more than 30 minutes between sample time points.

Enter a print frequency and press ENTER. The DISSO Parameters screen displays.

The print frequency timer restarts after each sample time point.

Note: The status does not print if the specified print frequency is less than three minutes from the sample time point; the sample time point takes precedence.

54 8000 Operator’s Manual

Operating the 8000 4

NOTE

Once all values have been entered, press ESC. A confirmation screen displays. Press Y to send the newly entered configuration to the dissolution apparatus and override the existing settings.

Main Menu Option 3 - Set Syringe Pump

This section is applicable only to systems including a syringe pump.

Ensure the syringe pump is enabled prior to setting the syringe pump parameters (see MENU > 0 > P under “Hidden Key Functions” on page

53).

From the Main Menu, select option 3, Set Syringe Pump. Select option 1 to enable the syringe pump. Once enabled, the Set Syringe Pump Parameters screen displays.

***SET SYRINGE PUMP PARAMETERS***

1 SYRINGE SIZE 2 PLUNGER SPEED

3 PRIME LOSS VOLUME 4 ASPIRT. DWELL TIME

Tabl e 6 Set Syringe Pump Parameters (Apparatus 1 / 2)

8000 Operator’s Manual 55

Following is a description of the Set Syringe Pump Parameters screen options:

Option Function

1 Syringe Size Use this option to set the syringe size.

Select option 1, Syringe Size. Select the syringe size that corresponds with the Kloehn syringes loaded into the syringe pump. The Set Syringe Pump Parameters screen displays.

2 Plunger Speed Use this option to configure the syringe pump plunger to operate at a specific

speed.

Select option 2, Plunger Speed. Enter a plunger speed between 100 and 1500 motor steps per second (sps) and press ENTER. The recommended value is 500. The Set Syringe Pump Parameters screen displays.

4 Operating the 8000

Tabl e 6 Set Syringe Pump Parameters (Apparatus 1 / 2)

Option Function

3 Prime Loss Volume Use this option to configure the prime loss volume. Prime loss volume

compensates for the volume of medium used to fill the tubing between the sampling cannulas and sampling valves during the priming cycle. When sampling, an amount of sample equal to the prime loss volume flows past the valves in the 8000 before the valves open to collect sample. The program also restricts this volume to be less than or equal to the syringe size volume.

Select option 3, Prime Loss Volume. Enter a prime loss volume based on the sample tubing length, inner diameter, and system dead volumes and press ENTER. The Set Syringe Pump Parameters screen displays.

If the tubing length between the sampling cannulas and sampling valves is changed, adjust the prime loss volume accordingly.

4 Aspirt. Dwell Time Use this option to configure the aspiration dwell time. Aspiration dwell time is the

time the syringe plunger pauses at the bottom of a stroke to allow the medium being drawn to overcome the vacuum generated by the drawn plunger.

Select option 4, Aspirt. Dwell Time. Enter an aspiration dwell time between 1 and 10 seconds and press ENTER The recommended time is 10 seconds. The Set Syringe Pump Parameters screen displays.

5 Full Stroke (hidden option)

Use this option to configure the number of steps the stepper motor makes to draw the syringe plunger its maximum stroke length.

Select option 5, Full Stroke. Enter a full stroke value between 0 and 19999 and press ENTER. The recommended value is 19300. The Set Syringe Pump Parameters screen displays.

If the volume dispensed by the syringe is outside of the required specifications, the full stroke value can be adjusted using the following calculation and solving for x:

target volume (mL) x 19300

= avg volume dispensed (mL)

6 Pump Current (hidden option)

Use this option to configure the pump current. The use of more viscous media requires a higher pump current.

Select option 6, Pump Current. Enter a pump current between 0 and 8 and press ENTER. The recommended value is 4. The Set Syringe Pump Parameters screen displays.

56 8000 Operator’s Manual

Main Menu Option 6 - Control DISSO

CAUTION

From the Main Menu, select option 6, Control DISSO. The DISSO Control/Monitor screen displays.

***DISSO CONTROL/MONITOR***

1 RUN 2 STOP 3 LIFT 4 LOWER 5 STILL

SET RPM: 100.0 ACTUAL RPM: 000.0

SET BATH TEMP: 37.5 ACTUAL TEMP: 37.5

The following options are commands communicated from the 8000 to the dissolution apparatus:

Tabl e 7 Disso Control/Monitor Options (Apparatus 1 / 2)

Option Function

1 Run Select option 1, Run, to start the paddle or basket rotation at the last set RPM.

2 Stop Select option 2, Stop, to stop the rotation of the paddles or baskets.

Operating the 8000 4

3 Lift Select option 3, Lift, to raise the dissolution apparatus drive unit.

4 Lower Select option 4, Lower, to lower the dissolution apparatus drive unit.

5 Still Select option 5, Still, to stop the upward or downward movement of the dissolution

apparatus drive unit.

All functions are executed immediately without pressing ENTER. Press ESC to return to the Main Menu.

After selecting option 3, Lift, or option 4, Lower, the drive unit can only be stopped by selecting option 5, Still, or by pressing DRIVE UP, DRIVE DOWN, or LIFT p or q on the dissolution apparatus.

Main Menu Option 7 - Manual Operation

From the Main Menu, select option 7, Manual Operation.

8000 Operator’s Manual 57

4 Operating the 8000

The following Manual Operations screen displays when your system configuration includes the peristaltic pump:

***MANUAL OPERATIONS***

1 LIFT VALVES 2 LOWER VALVES

3 OPEN/CLOSE VALVES 4 GOTO ROWS

5 TURN ON (OFF) R-M PUMP

The following Manual Operations screen displays when your system configuration includes the syringe pump with or without the filter changer:

***MANUAL OPERATIONS***

1 LIFT VALVES 2 LOWER VALVES

3 OPEN/CLOSE VALVES 4 GOTO ROWS

5 TURN ON (OFF) R-M PUMP 6 SYRINGE/FILTER

All functions execute immediately without pressing ENTER. Press ESC to return to the Main Menu.

Following is a description of the Manual Operations screen options:

Tabl e 8 Manual Operations Options (Apparatus 1 / 2)

Option Function

1 Lift Valves Select option 1, Lift Valves, to raise the valve needles if lowered.

2 Lower Valves Select option 2, Lower Valves, to lower the valve needles if raised.

3 Open/Close Valves Select option 3, Open/Close Valves, to toggle the valves open and closed.

4 Goto Rows Select option 4, Goto Rows, to move the 8000 dispensing arm to a specific

row. Enter a row number. The dispensing arm moves to the indicated row and stops.

If the moving alert is enabled, the alarm sounds when the dispensing arm moves. To disable the alert, see MENU > 0 > B under “Hidden Key

Functions” on page 41.

58 8000 Operator’s Manual

Tabl e 8 Manual Operations Options (Apparatus 1 / 2)

Option Function

Operating the 8000 4

5 Turn On (Off) R-M

Pump

6 Syringe/Filter (syringe pump only)

Select option 5, Turn On (Off) R-M Pump, to toggle the replacement media option off and on, if installed.

Use this option to manually control the syringe pump and filter changer as applicable. See “Manual Operation Option 6 - Syringe/Filter” below.

Manual Operation Option 6 - Syringe/Filter

From the Manual Operations screen, select option 6, Syringe/Filter. The Operate Syringe/Filter Changer screen displays.

***OPERATE SYRINGE/FILTER CHANGER***

1 VALVE TO INPUT 2 VALVE TO OUTPUT

3 SYRINGE FILL 4 SYRINGE DISPENSE

5 FILTER CHANGER 6 SYRINGE PARAMETERS

Following is a description of the Operate Syringe/Filter Changer screen options:

Tabl e 9 Operate Syringe/Filter Changer Options (Apparatus 1 / 2)

Option Function

1 Valve to Input The syringe pump contains a valve to control the direction of the flow of media.

Use this option to move the valve to the input position to manually input or fill the syringe with media.

Select option 1, Valve to Input, to move the valve to the input position. The valve emits a loud click when it moves. If you hear no sound, the valve is already in the input position.

2 Valve to Output The syringe pump contains a valve to control the direction of the flow of media.

Use this option to move the valve to the output position to manually output or purge the syringe of media.

Select option 2, Valve to Output, to move the valve to the output position. The valve emits a loud click when it moves. If you hear no sound, the valve is already in the output position.

8000 Operator’s Manual 59

4 Operating the 8000

Tabl e 9 Operate Syringe/Filter Changer Options (Apparatus 1 / 2)

Option Function

3 Syringe Fill Use this option to draw a specific amount of medium into the syringes. Ensure the

valve is in the input position before drawing the medium (“1 Valve to Input” on page 59).

Select option 3, Syringe Fill. Enter the desired percentage of each syringe to fill and press ENTER. The syringes draw the specified amount of medium.

4 Syringe Dispense Use this option to dispense medium from the syringes.

Select option 4, Syringe Dispense. The syringes dispense the previously drawn medium (see “3 Syringe Fill” above).

5 Filter Changer Use this option to manually operate the filter changer.

Select option 5, Filter Changer. The Filter Change Manual Control screen displays.

To open the filter clamp, select option 1, Open Clamp.

To close the filter clamp, select option 2, Close Clamp.

To change the filters, select option 3, Change Filters. Enter the number of filters to change and press ENTER. The specified number of filters are expelled and replaced.

Press ESC to return to the Operate Syringe/Filter Changer screen.

6 Syringe Parameters Use this option to configure the syringe pump. Select option 6, Syringe

Parameters. The Set Syringe Pump Parameters screen displays. For a description of the screen options, see “Main Menu Option 3 - Set Syringe Pump” on page 55.

Note: The syringe pump parameters are global. These parameters can be entered under option 3 of the Main Menu or option 6 of the Operate Syringe/Filter Changer screen.

60 8000 Operator’s Manual

Creating a Sample Program

NOTE

Programs are not write-protected. Check with other users before proceeding.

To program the dissolution apparatus with the 8000, you must enable external control on the dissolution apparatus and log out. For more information, see the applicable Operator’s Manual for your dissolution apparatus.

1 From the Ready screen, press PROG to program the 8000.

2 Enter a program number and press ENTER. The Program Variables

screen displays, as shown on page 61. Each option is explained on the following pages.

The 8000 can store up to 15 programs in non-volatile memory. Valid program numbers are 1 to 15. If 0 (zero) or a number larger than 15 is entered, the system continues to prompt you until a valid number is entered.

Operating the 8000 4

8000 Operator’s Manual 61

The following Program Variables screen displays when your system configuration includes the peristaltic pump:

***PROGRAM VARIABLES*** 4 HEADERS

1 SET DISSO PARAMETERS 5 PRIME TIME

2 SAMPLE TIME POINTS 6 PURGE TIME

3 SAMPLE VOLUME 7 R-M

4 Operating the 8000

NOTE

The following Program Variables screen displays when your system configuration includes the syringe pump with or without the filter changer:

***PROGRAM VARIABLES*** 4 HEADERS

1 SET DISSO PARAMETERS 5 PRIME VOLUME

2 SAMPLE TIME POINTS 6 PURGE VOLUME

3 SAMPLE VOLUME 7 FILTER CHG or R-M

If your system configuration includes the syringe pump and filter changer, option 7 displays as Filter Chg. See “7 Filter Chg (syringe

pump and filter changer)” on page 64.

If your system configuration includes the syringe pump but not the filter changer, option 7 displays as R-M. See “7 R-M (syringe pump)” on

page 64.

Following is a description of the Program Variables screen options:

Tabl e 1 0 Program Variables Options (Apparatus 1 / 2)

Option Function

1 Set DISSO Parameters Use this option to set the dissolution apparatus parameters. Select option 1, Set

DISSO Parameters. The DISSO Parameters screen displays. See “Main Menu Option

2 - Set DISSO” on page 53 for a description of the DISSO Parameters screen options.

2 Sample Time Points Use this option to enter the sample time points. See “Program Option 2 - Sample

Time Points” on page 65.

3 Sample Volume Select option 3, Sample Volume. Enter the sample volume in milliliters and press

ENTER. The program Variables screen displays. You can enter the volume with up

to three digits (for example 13.5) so that fractional milliliters can be collected. Verify the collection tubes can hold the requested volume.

Note: The maximum volume accepted is 14 mL.

62 8000 Operator’s Manual

Operating the 8000 4

Tabl e 1 0 Program Variables Options (Apparatus 1 / 2)

Option Function

4 Headers Select option 4, Headers. The Batch # Selections screen displays. For each option,

enter the desired information and press ENTER. Press ESC to return to the Program Variables screen.

Note: The Batch # Selections screen displays options for the report header information. The print field accepts ten characters. Characters can be letters, numbers, special characters or spaces. The information entered for each selection prints in the header of each printout. The entries made from this menu are attached to the active program. Since there are 15 programs, there can be a different set of entries for each. All are stored in the battery-protected memory and remain until changed via this menu.

5 Prime Time (peristaltic pump)

5 Prime Volume (syringe pump)

6 Purge Time (peristaltic pump)

Select option 5, Prime Time. Enter a value for the priming time from 1 to 99 seconds and press ENTER. The Program Variables screen displays.

Note: At a minimum, the prime time should be long enough for medium to be seen dripping from the return cannulas. In general, the longer the tubing lengths, the longer the priming time required.

If your system configuration includes the syringe pump, use this option to set the amount of drawn medium necessary to fill the sampling lines of the entire system.

Select option 5, Prime Volume. Enter a value for the priming volume and press ENTER. The Program Variables screen displays.

Note: At a minimum the prime volume should be large enough for medium to be seen dripping from the return cannulas. In general, the longer the tubing lengths, the larger the prime volume required.

Select option 6, Purge Time. Enter a value for the purge time from 1 to 99 seconds and press ENTER. The Program Variables screen displays.

Note: Purging also clears the sampling filters of particulate matter that may restrict successive samples. As with priming, the longer the tubing lengths, the longer the purge time required.

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4 Operating the 8000

Tabl e 1 0 Program Variables Options (Apparatus 1 / 2)

Option Function

6 Purge Volume (syringe

pump)

7 R-M (peristaltic pump) If your system configuration includes the peristaltic pump, select option 7, R-M. The

7 Filter Chg (syringe pump

and filter changer)

If your system configuration includes the syringe pump, use this option to set a purge volume that ensures all stranded medium is properly expelled.

Select option 6, Purge Volume. Enter a value for the purge volume that ensures at least two strokes of the syringe plunger and press ENTER. The Program Variables screen displays.

Replacement Media Option screen displays. Select option 1, Enable. Enter the desired percentage of replacement medium and press ENTER. Optionally, select option 2, Disable. The Program Variables screen displays.

Note: If the RM option is not installed, select option 2, Disable.

If your system configuration includes the syringe pump and filter changer, select option 7, Filter Chg. Enter the desired number of samples each filter should process before being discharged and press ENTER. The recommended value is 1. The Program Variables screen displays.

Note: If your system configuration includes the syringe pump and filter changer, ensure the syringe pump option is enabled. From the Ready screen, press MENU. Select option 3, Set Syringe Pump. Select option 1, Enable. Also press MENU > 0 > P and select option 1, Enable.

The replacement media option is not available with this system configuration. Ensure the RM option for the syringe pump is disabled using the hidden key function MENU > 0 > R.

7 R-M (syringe pump)

If your system configuration includes the syringe pump but not the filter changer, select option 7, R-M. The Replacement Media Option screen displays. Select option 1, Enable, or option 2, Disable. If the RM option is not installed, select option 2, Disable. The Program Variables screen displays.

Note: If your system configuration includes the syringe pump, ensure the syringe pump option is enabled. From the Ready screen, press MENU. Select option 3, Set Syringe Pump. Select option 1, Enable.

If your system configuration includes the syringe pump but not the filter changer, use the hidden key function MENU > 0 > R to enable or disable the RM option, as appropriate. If the RM option is not installed, select option 2, Disable.

1, Enable. Also press MENU > 0 > P and select option

64 8000 Operator’s Manual

Operating the 8000 4

NOTE

Program Option 2 - Sample Time Points

From the Program Variables screen, select option 2, Sample Time Points. The following screen displays:

SAMPLE TIME POINT: 000:02

ENTER hhh:mm UP TO 999:59 HOURS

PROG #: 1 ROW: 0 VOLUME: 5ml

TIME:01/01/12 09:37:14 ELAPSE:000:00:00

This screen accepts sampling times and saves them for execution when the program is run. Time points are requested in order of execution starting with collection Row 0 on the sample tray. The sample time is entered as hours and minutes. Once you enter a time, the row number increments and another sample time point is requested.

The following conventions are used with this screen:

• Review previously entered sample time points by pressing ENTER

to scroll through the entries.

• After all desired sample time points have been entered, enter 000:00

for the last sample time point and press ENTER. If the maximum of ten sample time points are entered, you do not have to enter 000:00 for the last sample time point.

8000 Operator’s Manual 65

If a sample time point is required after the final spin (250 RPM), enter a maximum of nine sample time points. The firmware accounts for the final spin sample, but the tenth space must be left blank.

• To add additional sample time points, press ENTER until a new row

time displays. Enter the additional sample time point.

• If more than ten sample time points are needed, use the link program

feature. See “Starting a 8000 Program” on page 66.

• To correct an entry, press BACKSPACE to go to the previous

character position and re-enter the correct value.

4 Operating the 8000

NOTE

Starting a 8000 Program

• If a shorter sample time point than the previous one is entered for a

given row, the row number will not increment. Times must increase from one row to the next.

• To shorten a saved program, enter 000:00 for the first sample time

point you wish to discard. All successive time points are eliminated.

• Press ESC to save all new values and return to the Program Variables

screen.

Sample time points should be at least five minutes apart to allow adequate time for priming and purging the sampling lines. If your system configuration includes the syringe pump and filter changer, ensure the sample time points are at least six minutes apart.

1 From the Ready screen, press START PROG to start a program or

modify a program already operating. The Enter Prog screen displays.

If using baskets or rotating cylinders, ensure the drive unit of the dissolution apparatus is raised to its highest position before starting the 8000 program.

66 8000 Operator’s Manual

If using a dissolution apparatus with the 8000, you must enable external control on the dissolution apparatus and log out.

2 Enter a program number between 1 and 15 and press ENTER. The

Link Program Option screen displays.

***LINK PROGRAM OPTION***

1 RUN SINGLE PROG 2 LINK ANOTHER PROG

TIME: 01/01/12 15:02:30 ELAPSE: 000:00:04

Operating the 8000 4

NOTE

3 To run a single program, select option 1.

If more than ten rows are required to collect samples, linking a program allows you to run two programs with identical test parameters back to back. To link identical programs, link the program to itself.

To link another program to the one previously selected, select option 2. The following screen displays:

LINK PROG #: (1-15):__

TIME: 01/01/12 15:02:30 ELAPSE: 000:00:04

Enter the program number to link to the current program and press ENTER.

When running two programs, it is necessary to replace the full sample tray with an empty one between programs. Enable the program completion alarm (see “5 Prg Compl Alarm” under “Main Menu Option 1 Set Clock/Alarms” on 40) to alert the analyst when the first program completes and the second program begins.

8000 Operator’s Manual 67

4 The Select Start Mode screen displays. Options 1 - 3 start the

collection program. Option 4 allows you to change or review the program before beginning.

***SELECT START MODE***

1 START NOW 2 DELAYED START

3 REMOTE START 4 MODIFY PROGRAM

PROG#: 1 R-M: DISABLED VOL: 5 ml

4 Operating the 8000

Following is a description of the Select Start Mode screen options:

Tabl e 11 Select Start Mode Options (Apparatus 1 / 2)

Option Function

1 Start Now Select option 1, Start Now. The DISSO Options screen displays. See “DISSO

Options Screen” on page 69.

2 Delayed Start Select option 2, Delayed Start. The DISSO Options screen displays. See “DISSO

Options Screen” on page 69.

3 Remote Start This option is designed for use with the 8000 coupled with non-Agilent

dissolution apparatus. Contact the Dissolution Systems Service Department for information about this function.

4 Modify Program Select option 4, Modify Program, to modify or review the selected program. See

“Creating a Sample Program” on page 61 to review programming options. All

program variable items can be viewed or changed.

Press ESC to return to the Select Start Mode screen.

Selecting option 4 again repeats the modify program operation.

When the program is verified or changed to the correct configuration, select the desired start mode (option 1 - 3) to start the program.

68 8000 Operator’s Manual

DISSO Options Screen

***DISSO OPTIONS***

1 LOAD & RUN DISSO 2 RUN WO/ LOADING

3 RUN 8000 ONLY 4 SET DISSO

TIME: 01/01/12 08:09:51 ELAPSE: 000:00:00

Following is a description of the DISSO Options screen options:

Tabl e 1 2 Disso Options (Apparatus 1 / 2)

Option Start Now Function Delayed Start Function

Operating the 8000 4

1 Load & Run

DISSO

2 Run WO/ Loading

Use this option to upload all saved parameters to the dissolution apparatus and start the program. Select option 1, Load & Run DISSO. The Select DISSO Program Start Mode screen displays. See “Select DISSO Program

Start Mode Screen” on page 70.

Use this option to program and run the 8000 without loading and overriding dissolution apparatus settings. Select option 2, Run WO/ Loading. The Select DISSO Program Start Mode screen displays. See “Select DISSO

Program Start Mode Screen” on

page 70.

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4 Operating the 8000

Tabl e 1 2 Disso Options (Apparatus 1 / 2)

Option Start Now Function Delayed Start Function

3 Run 8000

Only

4 Set DISSO Use this option to modify or review

Select option 3, Run 8000 Only, to immediately start the 8000 only. The 8000 executes the program currently selected.

This option is used when other instrumentation, such as a dissolution apparatus, is connected to the 8000 through the START OUTPUT jack on the rear of the instrument.

the current program parameters. Select option 4, Set DISSO. The Set DISSO Parameters screen displays. See “Main

Menu Option 2 - Set DISSO” on

page 53 for a description of the DISSO Parameters screen options.

Select DISSO Program Start Mode Screen

Select option 3, Run 8000 Only, to start the 8000 only.

Enter the start time in 24-hour format and press ENTER. Enter the start date in the appropriate format and press ENTER. The DISSO Options screen displays.

If the delayed start alarm is enabled, enter the duration before the start of the program you would like the alarm to sound. To disable the alarm, see MENU > 0 > C under

“Hidden Key Functions” on page 41.

This option is used when other instrumentation, such as a dissolution apparatus, is connected to the 8000 through the START OUTPUT jack on the rear of the instrument.

***SELECT DISSO PROGRAM START MODE***

1 INSTANT 2 SET VESSEL TEMP AND START

TIME: 01/01/12 08:09:51 ELAPSE: 000:00:00

Following is a description of the Select DISSO Program Start Mode screen options:

70 8000 Operator’s Manual

Tabl e 1 3 Select Disso Program Start Mode Options (Apparatus 1 / 2)

Option Start Now Function Delayed Start Function

Operating the 8000 4

1 Instant Select option 1, Instant, to start

the dissolution apparatus instantly. To stop the dissolution apparatus, press ESC. A confirmation screen displays. Press Y to stop the program. Press N to continue running the program.

2 Set Vessel Temp And Start

Select option 2, Set Vessel Temp And Start, to set the temperature the vessels have to reach to initiate the test. Enter the desired vessel temperature and press ENTER. The program starts once the set vessel temperature is reached.

Select option 1, Instant, to set the desired time to start the dissolution apparatus.

Enter the start time in 24-hour format and press ENTER.

Enter the start date in the appropriate format and press ENTER. The screen displays the program information, including the entered start time and date.

If the delayed start alarm is enabled, enter the duration before the start of the program you would like the alarm to sound. To disable the alarm, see MENU > 0 > C under

“Hidden Key Functions” on page 41.

Press ESC to cancel the delayed start.

Select option 2, Set Vessel Temp And Start, to set the temperature the vessels have to reach to initiate the test.

Enter the desired vessel temperature and press ENTER.

Enter the start time in 24-hour format and press ENTER.

Enter the start date in the appropriate format and press ENTER. The screen displays the program information, including the entered start time and date.

If the delayed start alarm is enabled, enter the duration before the start of the program you would like the alarm to sound. To disable the alarm, see MENU > 0 > C under

“Hidden Key Functions” on page 41.

Press ESC to cancel the delayed start.

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4 Operating the 8000

Operating the 8000 with Apparatus 3 / 7

8000 Keypad Options

The keypad on the 8000 is similar to a personal computer keyboard. For example, it has a SPACE key, an ENTER key, and a SHIFT key. These keys function exactly like their counterparts on a personal computer or typewriter keyboard. See Figure 15, “8000 Keypad,” below. A discussion about each of the main function keys follows.

Figure 15 8000 Keypad

72 8000 Operator’s Manual

The front panel options include the following:

Tabl e 1 4 Front Panel Options (Apparatus 3 / 7)

Option Function

ESC Press ESC to stop execution of a running program or calibration routine. Press

ESC to move back one level to the previous menu or selection when moving

through the multi-level menu.

Be very careful when using ESC while a program is running. If a program or calibration is running and ESC is pressed, a prompt appears on the first line of the display asking if you want to stop the running program. The options are Y or N. Press Y to stop the running program. Press N to continue the running program.

MENU Use this option to set the clock, alarms, Apparatus 3 / Apparatus 7 parameters, start

output delay / syringe pump parameters, manual prime time / volume, manual purge time / volume as well as to remotely control the Apparatus 3 / 7 and manually control the 8000. See “8000 with Apparatus 3 / 7 - Menu Options” on page 74.

PRINT Press PRINT to record the batch information, print the dip and drain times, enable

or disable the Report Center Printer, select a local or remote printer, set the communication port identification number, and set the baud rate. See “Printer

Operation and Communications” on page 103.

Operating the 8000 4

CAL Use this option to calibrate the 8000 to ensure that sample volumes are accurately

and precisely taken. See “Volumetric Calibration (Peristaltic Pump only)” on page 97.

PROG Use this option to set program parameters. See “Creating a Sample Program” on

page 61.

START PROG Use this option to start a program. See “Starting a Program” on page 91.

MANUAL SAMPLE Use this option to collect a sample from the dissolution vessels on command

without a program. See “Manual Sample” on page 95.

OPEN VALVES Press OPEN VALVES to open all valves simultaneously. This function keeps the

valves open as long as the key on the keypad is pressed. To change the valve configuration, press MENU > 0 > 0 and select the appropriate configuration.

PUMP OFF

PUMP FWD

PUMP REV

PUMP OFF, PUMP FWD, and PUMP REV work exclusively with the Agilent peristaltic pump. When the syringe pump is connected, they are disabled.

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4 Operating the 8000

Tabl e 1 4 Front Panel Options (Apparatus 3 / 7)

Option Function

CLEAN SYTEM Use this option to keep the valves, needles, and fluid lines clean. See “Clean

System with Peristaltic Pump” on page 106 or “Clean System Function with Syringe Pump” on page 111.

LOCK KBRD Press LOCK KBRD to lock the keypad. Press LOCK KBRD again to unlock the

keypad.

8000 with Apparatus 3 / 7 - Menu Options

Main Menu

From the Ready screen, press MENU. The Main Menu displays. Options 3, 4, and 5 change depending on your system configuration.

When your system configuration includes the peristaltic pump, the following Main Menu displays:

***MAIN MENU*** 4 MANUAL PRIME TIME

1 SET CLOCK/ALARMS 5 MANUAL PURGE TIME

2 SET APP 3/7 6 CONTROL APP 3/7

3 START OUTPUT DELAY 7 MANUAL OPERATION

When your system configuration includes the syringe pump with or without the filter changer, the following Main Menu displays:

***MAIN MENU*** 4 MANUAL PRIME VOL.

1 SET CLOCK/ALARMS 5 MANUAL PURGE VOL.

2 SET APP 3/7 6 CONTROL APP 3/7

3 SET SYRINGE PUMP 7 MANUAL OPERATION

74 8000 Operator’s Manual

Operating the 8000 4

Following is a description of the Main Menu screen options:

Tabl e 1 5 Main Menu Options (Apparatus 3 / 7)

Option Function

1 Set Clock/Alarms Use this option to set the clock and alarms. If your system configuration includes the

syringe pump, the start output delay option also displays on this screen. See “Main

Menu Option 1 - Set Clock/Alarms” on page 77.

2 Set APP 3/7 Use this option to set the Apparatus 3 / 7 parameters. See “Main Menu Option 2 - Set

DISSO” on page 53.

3 Start Output Delay (peristaltic pump)

3 Set Syringe Pump (syringe pump)

4 Manual Prime Time (peristaltic pump)

Select option 3, Start Output Delay. Enter a delay length of up to 99:59 minutes for the 8000 TTL output and press ENTER. The Main Menu displays.

Note: This output signal can be used to start other instrumentation, such as an Apparatus 3 / 7, after the program on the 8000 is started. The default delay is 00:00, indicating that the signal will be sent at the moment a program starts. For example, a value of 10:00 delays the signal for ten minutes after the program starts. Set the value to 00:00 to eliminate the delay feature.

When the syringe pump is enabled, Start Output Delay displays under the Set Clock and Alarms screen as option 6. See “6 Start Output Delay” on page 52.

If your system configuration includes the syringe pump, use this option to set the syringe pump parameters. See “Main Menu Option 3 - Set Syringe Pump” on page 80.

Select option 4, Manual Prime Time. Enter a value for the priming time from 1 to 99 seconds and press ENTER. The Main Menu displays.

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4 Operating the 8000

Tabl e 1 5 Main Menu Options (Apparatus 3 / 7)

Option Function

4 Manual Prime Vol.

(syringe pump)

5 Manual Purge Time (peristaltic pump)

5 Manual Purge Vol. (syringe pump)

If your system configuration includes the syringe pump, use this option to set the amount of drawn medium necessary to fill the sampling lines of the entire system.

Select option 4, Manual Prime Vol. Enter a value for the priming volume and press ENTER. The Main Menu displays.

Select option 5, Manual Purge Time. Enter a value for the manual purge time from 1 to 99 seconds and press ENTER. The Main Menu displays.

If your system configuration includes the syringe pump, use this option to set the a purge volume that ensures all stranded medium is properly expelled.

Select option 5, Manual Purge Vol. Enter a value for the purge volume that ensures at least two strokes of the plunger and press ENTER. The Main Menu displays.

Note: The first stroke moves all medium from the sampling lines to the return lines. The second stroke draws air into the sampling lines and purges the remaining medium from the return lines. As with priming, the longer the tubing lengths, the larger the purge volume required. This value is not used by sampling programs which have their own individual purge volume values. It is used only with the MANUAL SAMPLE and CLEAN SYSTEM keys on the 8000.

6 Control APP 3/7 Use this option to remotely control the Apparatus 3 / 7 from the 8000. See “Main

Menu Option 6 - Control APP 3/7” on page 82.

7 Manual Operation Use this option to manually control the 8000. See “Main Menu Option 7 - Manual

Operation” on page 83.

76 8000 Operator’s Manual

Operating the 8000 4

Main Menu Option 1 - Set Clock/Alarms

From the Main Menu, select option 1, Set Clock/Alarms. The Set Clock and Alarms screen displays.

***SET CLOCK AND ALARMS***

1 SET CLOCK 2 TIMER ALARM

3 ROW COMPL ALARM 4 CALIB. COMPL ALARM

5 PRG COMPL ALARM 6 START OUTPUT DELAY

Following is a description of the Set Clock and Alarms screen options:

Tabl e 1 6 Set Clock and Alarms Options

Option Function

1 Set Clock Select option 1, Set Clock. Enter the date in the appropriate format and press

ENTER. The time displays in 24-hour format. If the time is correct, press ENTER or ESC to return to the Main Menu. If the time is incorrect, enter the correct time in 24-hour format and press ENTER. The Set Clock and Alarms screen displays.

2 Timer Alarm Use this option to set the elapsed timer alarm. The elapsed timer alarm is a simple

timer you can use to time any interval from 1 minute to 99 hours. The alarm sounds when the time has expired. Once turned off, the timer does not operate again until you reset it.

Select option 2, Timer Alarm. Enter a duration in hh:mm format and press ENTER. The Set Clock and Alarms screen displays.

Press any key to silence the alarm after it sounds.

3 Row Compl Alarm Use this option to set an alarm to indicate the completion of each sample time point.

This alarm is useful when the samples must be checked or removed for immediate analysis.

Select option 3, Row Compl Alarm. Select option 1 to enable or option 2 to disable the alarm. The Set Clock and Alarms screen displays.

Press any key to silence the alarm after it sounds.

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4 Operating the 8000

Tabl e 1 6 Set Clock and Alarms Options

Option Function

4 Calib. Compl Alarm Use this option to set an alarm to sound when autocalibration is complete and all lines

are purged.

Select option 4, Calib. Compl Alarm. Select option 1 to enable or option 2 to disable the alarm. The Set Clock and Alarms screen displays.

Press any key to silence the alarm after it sounds.

Note: This option is only available if your system configuration includes the peristaltic pump.

5 Prg Compl Alarm Use this option to set an alarm to sound at the end of the program. It is a useful

reminder that the program is complete.

Select option 5, Prg Compl Alarm. Select option 1 to enable or option 2 to disable the alarm. The Set Clock and Alarms screen displays.

Press any key to silence the alarm after it sounds.

6 Start Output Delay Note: Option 6 displays only when the syringe pump is enabled.

If the syringe pump is enabled, use this option to set the delay from the start of a program until the TTL signal is sent from the START OUTPUT jack on the 8000 rear panel.

When the syringe pump is disabled, this option displays under the Main Menu. See “3

Start Output Delay (peristaltic pump)” on page 49.

78 8000 Operator’s Manual

Main Menu Option 2 - Set APP 3/7

From the Main Menu, select option 2, Set APP 3/7. Enter a program number and press ENTER. The Set APP 3/7 Parameters screen displays.

***SET APP 3/7 PARAMETERS***

1 DIPPING SPEED (DPM) 2 SAMPLES PER ROW

3 HOLD DIP TIME 4 SET BATH TEMP

5 COM PORT ID 6 PRINT FREQUENCY

Following is a description of the Set APP 3/7 Parameters screen options:

Tabl e 1 7 Set App 3/7 Parameters Options (Apparatus 3 / 7)

Option Function

1 Dipping Speed (DPM) Select option 1, Dipping Speed (DPM). Enter the desired DPM and press

ENTER. The Set APP 3/7 Parameters screen displays.

2 Samples Per Row Select option 2, Samples Per Row. Enter the desired number of samples to be

taken from each row and press ENTER. The Set APP 3/7 Parameters screen displays.

Operating the 8000 4

3 Hold Dip Time Select option 3, Hold Dip Time. Enter a hold dip time and press ENTER to

advance to each remaining row. After a hold dip time is entered for the final row, the Set APP 3/7 Parameters screen displays.

4 Set Bath Temp Select option 4, Set Bath Temp. Enter the desired water bath temperature and

press ENTER. The Set APP 3/7 Parameters screen displays.

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4 Operating the 8000

NOTE

Tabl e 1 7 Set App 3/7 Parameters Options (Apparatus 3 / 7)

Option Function

5 Com Port ID Select option 5, Com Port ID, to change the communication port identification

Enter a communication port identification number and press ENTER. The Set APP 3/7 Parameters screen displays.

6 Print Frequency The Apparatus 3 / 7 status prints at each sample time point. Select option 6, Print

Frequency, to set additional times to print the Apparatus 3 / 7 status between sample time points. This option should be reserved for use when there is more than 30 minutes between sample time points.

Enter a print frequency and press ENTER. The Set APP 3/7 Parameters screen displays.

The print frequency timer resets after each sample time point.

Note: The status does not print if the specified print frequency time falls within three minutes of the sample time point; the sample time point takes precedence.

Once all values have been entered, press ESC. A confirmation screen displays. Press Y to send the newly entered parameters to the Apparatus 3 / 7 and override the existing settings.

80 8000 Operator’s Manual

Main Menu Option 3 - Set Syringe Pump

This section is applicable only to systems including a syringe pump.

Ensure the syringe pump is enabled prior to setting the syringe pump parameters (see MENU > 0 > P under page 41

“Hidden Key Functions” on

Operating the 8000 4

From the Main Menu, select option 3, Set Syringe Pump. Select option 2 to disable the syringe pump or select option 1 to enable the syringe

pump. Once enabled, the Set Syringe Pump Parameters screen displays.

***SET SYRINGE PUMP PARAMETERS***

1 SYRINGE SIZE 2 PLUNGER SPEED

3 PRIME LOSS VOLUME 4 ASPIRT. DWELL TIME

Following is a description of the Set Syringe Pump Parameters screen options:

Tabl e 1 8 Set Syringe Pump Parameters Options (Apparatus 3 / 7)

Option Function

1 Syringe Size Use this option to set the syringe size.

Select option 1, Syringe Size. Select the syringe size that corresponds with the Kloehn syringes loaded into the syringe pump. The Set Syringe Pump Parameters screen displays.

2 Plunger Speed Use this option to configure the syringe pump plunger to operate at a specific speed.

Select option 2, Plunger Speed. Enter a plunger speed between 100 and 1500 motor steps per second (sps) and press ENTER. The recommended value is 500. The Set Syringe Pump Parameters screen displays.

3 Prime Loss Volume Use this option to configure the prime loss volume. Prime loss volume compensates

for the volume of medium used to fill the tubing between the sampling cannulas and sampling valves during the priming cycle. When sampling, an amount of sample equal to the prime loss volume flows past the valves in the 8000 before the valves open to collect sample. The program also restricts this volume to be less than or equal to the syringe size volume.

If the tubing length between the sampling cannulas and sampling valves is changed, adjust the prime loss volume accordingly.

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4 Operating the 8000

Tabl e 1 8 Set Syringe Pump Parameters Options (Apparatus 3 / 7)

Option Function

4 Aspirt. Dwell Time Use this option to configure the aspiration dwell time. Aspiration dwell time is the

time the syringe plunger pauses at the bottom of a stroke to allow the medium being drawn to overcome the vacuum generated by the drawn plunger.

Select option 4, Aspirt. Dwell Time. Enter an aspiration dwell time between 1 and 10 seconds and press ENTER. The recommended time is 10 seconds. The Set Syringe Pump Parameters screen displays.

5 Full Stroke (hidden option)

6 Pump Current (hidden option)

Use this option to configure the number of steps the stepper motor makes to draw the syringe plunger its maximum stroke length.

Select option 5, Full Stroke. Enter a full stroke value between 0 and 19999 and press ENTER. The recommended value is 19300. The Set Syringe Pump Parameters screen displays.

If the volume dispensed by the syringe is outside of the required specifications based on a 10 mL syringe size, the full stroke value can be adjusted using the following calculation and solving for x:

target volume (mL) x 19300

Use this option to configure the pump current. The use of more viscous media requires a higher pump current.

Select option 6, Pump Current. Enter a pump current between 0 and 8 and press ENTER. The recommended value is 4. The Set Syringe Pump Parameters screen displays.

= avg volume dispensed (mL)

Main Menu Option 6 - Control APP 3/7

From the Main Menu, select option 6, Control APP 3/7. The APP 3/7 Control/Monitor screen displays.

***APP 3/7 CONTROL/MONITOR***

1 DIP 2 STOP 3 GO TO ROW 4 HOME

SET RPM: 50 ACTUAL RPM: 000.0

SET BATH TEMP: 37.5 ACTUAL TEMP: 37.2

82 8000 Operator’s Manual

The following options are commands communicated from the 8000 to the Apparatus 3 / Apparatus 7:

Tabl e 1 9 App 3/7 Control/Monitor Options

Option Function

1 Dip Select option 1, Dip, to begin dipping.

2 Stop Select option 2, Stop, to stop dipping and raise the cannulas.

3 Go To Row Select option 3, Go To Row, to move the drive unit to a specific row. Enter a

row number and press ENTER. The Apparatus 3 / 7 drive unit moves to the indicated row.

4 Home Select option 4, Home, to move the drive unit to the home position.

Press ESC to return to the Main Menu.

Main Menu Option 7 - Manual Operation

From the Main Menu, select option 7, Manual Operation.

The following Manual Operations screen displays when your system configuration includes the peristaltic pump:

Operating the 8000 4

***MANUAL OPERATIONS***

1 LIFT VALVES 2 LOWER VALVES

3 OPEN/CLOSE VALVES 4 GOTO ROWS

5 TURN ON (OFF) R-M PUMP

The following Manual Operations screen displays when your system configuration includes the syringe pump with or without the filter changer:

***MANUAL OPERATIONS***

1 LIFT VALVES 2 LOWER VALVES

3 OPEN/CLOSE VALVES 4 GOTO ROWS

5 TURN ON (OFF) R-M PUMP 6 SYRINGE/FILTER

All functions execute immediately without pressing ENTER. Press ESC to return to the Main Menu.

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4 Operating the 8000

Following is a description of the Manual Operations screen options:

Tabl e 2 0 Manual Operations (Apparatus 3 / 7)

Option Function

1 Lift Valves Select option 1, Lift Valves, to raise the valve needles if lowered.

2 Lower Valves Select option 2, Lower Valves, to lower the valve needles if raised.

3 Open/Close Valves Select option 3, Open/Close Valves, to toggle the valves open and closed.

4 Goto Rows Select option 4, Goto Rows, to move the 8000 dispensing arm to a specific row.

Enter a row number. The dispensing arm moves to the indicated row and stops.

If the moving alert is enabled, the alarm sounds when the dispensing arm moves. To disable the alert, see MENU > 0 > B under “Hidden Key

Functions” on page 41.

5 Turn On (Off) R-M Pump

6 Syringe/Filter (syringe pump only)

TURN ON R-M PUMP displays as option 5. This indicates the replacement

Ensure media option is disabled. If TURN OFF R-M PUMP displays, select option 5. TURN

ON R-M PUMP displays.

Note: The replacement media option must be disabled or the instrument will not operate properly.

Use this option to manually control the syringe pump and filter changer as applicable. See “Manual Operation Option 6 - Syringe/Filter” below.

Manual Operation Option 6 Syringe/Filter

From the Manual Operations screen, select option 6, Syringe/Filter. The Operate Syringe/ Filter Changer screen displays.

***OPERATE SYRINGE/FILTER CHANGER***

1 VALVE TO INPUT 2 VALVE TO OUTPUT

3 SYRINGE FILL 4 SYRINGE DISPENSE

5 FILTER CHANGER 6 SYRINGE PARAMETERS

84 8000 Operator’s Manual

Operating the 8000 4

Following is a description of the Operate Syringe/Filter Changer screen options:

Tabl e 2 1 Operate Syringe/Filter Changer Options

Option Function

1 Valve to Input The syringe pump contains a valve to control the direction of the flow of medium.

Use this option to move the valve to the input position to manually input or fill the syringe with medium.

Select option 1, Valve to Input, to move the valve to the input position. The valve emits a loud click when it moves. If you hear no sound, the valve is already in the input position.

2 Valve to Output The syringe pump contains a valve to control the direction of the flow of medium.

Use this option to move the valve to the output position to manually output or purge the syringe of medium.

Select option 2, Valve to Output, to move the valve to the output position. The valve emits a loud click when it moves. If you hear no sound, the valve is already in the output position.

3 Syringe Fill Use this option to draw a specific amount of medium into the syringes. Ensure the

valve is in the input position before drawing the medium (see “1 Valve to Input” above).

Select option 3, Syringe Fill. Enter the desired percentage of each syringe to fill and press ENTER. The syringes draw the specified amount of medium.

4 Syringe Dispense Use this option to dispense medium from the syringes.

Select option 4, Syringe Dispense. The syringes dispense the previously drawn medium (see “3 Syringe Fill” above).

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4 Operating the 8000

NOTE

Tabl e 2 1 Operate Syringe/Filter Changer Options

Option Function

5 Filter Changer Use this option to manually operate the filter changer.

Select option 5, Filter Changer. The Filter Change Manual Control screen displays.

To open the filter clamp, select option 1, Open Clamp.

To close the filter clamp, select option 2, Close Clamp.

To change the filters, select option 3, Change Filters. Enter the number of filters to change and press ENTER. The specified number of filters are expelled and replaced.

Press ESC to return to the Operate Syringe/Filter Changer screen.

6 Syringe Parameters Use this option to configure the syringe pump. Select option 6, Syringe Parameters.

The Set Syringe Pump Parameters screen displays. For a description of the screen options, see “Main Menu Option 3 - Set Syringe Pump” on page 55.

Note: The syringe pump parameters are global. These parameters can be entered under option 3 of the Main Menu or option 6 of the Operate Syringe/Filter Changer screen.

Creating a Sample Program

86 8000 Operator’s Manual

Programs are not write-protected. Check with other users before proceeding.

1 From the Ready screen, press PROG to program the 8000.

2 Enter a program number and press ENTER. The Program Variables

screen displays, as shown below.

Operating the 8000 4

NOTE

The following Program Variables screen displays when your system configuration includes the peristaltic pump:

***PROGRAM VARIABLES*** 4 HEADERS

1 SET APP 3/7 PARAMETERS 5 PRIME TIME

2 SAMPLE TIME POINTS 6 PURGE TIME

3 SAMPLE VOLUME 7 R-M

The following Program Variables screen displays when your system configuration includes the syringe pump with or without the filter changer:

***PROGRAM VARIABLES*** 4 HEADERS

1 SET APP 3/7 PARAMETERS 5 PRIME VOLUME

2 SAMPLE TIME POINTS 6 PURGE VOLUME

3 SAMPLE VOLUME 7 FILTER CHG or R-M

Option 7 displays as Filter Chg when your system configuration includes the syringe pump and filter changer. See “7 Filter Chg (syringe pump and filter changer)” on page 51.

If your system configuration includes the syringe pump but not a filter changer, option 7 displays as R-M. See “7 R-M (syringe pump)” on page 51.

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4 Operating the 8000

Following is a description of the Program Variables screen options:

Tabl e 2 2 Program Variables Options (Apparatus 3 / 7)

Option Function

1 Set APP 3/7 Parameters Use this option to set the Apparatus 3 / 7 parameters. Select option 1, Set APP 3/7

Parameters. The Set APP 3/7 Parameters screen displays. See “Main Menu Option 2 -

Set DISSO” on page 53 for a description of the Set APP 3/7 Parameters screen options.

2 Sample Time Points Use this option to enter the sample time points. See “Program Option 2 - Sample Time

Points” on page 90.

3 Sample Volume Select option 3, Sample Volume. Enter the sample volume in milliliters and press

ENTER. The Program Variables screen displays. You can enter the volume with up to

three digits (for example 13.5) so that fractional milliliters can be collected. Verify the collection tubes can hold the requested volume.

Note: The maximum volume accepted is 14 mL.

4 Headers Select option 4, Headers. The Batch # Selections screen displays. For each option, enter

the desired information and press ENTER. The Program Variables screen displays.

Note: The Batch # Selections screen displays options for the report header information. The print field accepts ten characters. Characters can be letters, numbers, special characters, or spaces. The information entered for each selection prints in the header of each printout. The entries made from this menu are attached to the active program. Since there are 15 programs, there can be a different set of entries for each. All are stored in the battery-protected memory and remain until changed via this menu.

5 Prime Time (peristaltic pump)

5 Prime Volume (syringe pump)

Select option 5, Prime Time. Enter a value for the priming time from 1 to 99 seconds and press ENTER. The Program Variables screen displays.

Note: At a minimum, the prime time should be long enough for medium to be seen dripping from the return cannulas. In general, the longer the tubing lengths, the longer the priming time required.

If your system configuration includes the syringe pump, use this option to set the amount of drawn medium necessary to fill the sampling lines of the entire system.

Select option 5, Prime Volume. Enter a value for the priming volume and press ENTER. The Program Variables screen displays.

Note: At a minimum, the prime volume should be large enough for medium to be seen dripping from the return cannulas. In general, the longer the tubing lengths, the larger the prime volume required.

88 8000 Operator’s Manual

Tabl e 2 2 Program Variables Options (Apparatus 3 / 7)

Option Function

Operating the 8000 4

6 Purge Time (peristaltic

pump)

6 Purge Volume (syringe pump)

7 R-M (peristaltic pump) If your system configuration includes the peristaltic pump, select option 7, R-M. The

Select option 6, Purge Time. Enter a value for the purge time from 1 to 99 seconds and press ENTER. The Program Variables screen displays.

Note: Purging also clears the sampling filters of particulate matter that may restrict successive samples. As with priming, the longer the tubing lengths, the longer the purge time required.

If your system configuration includes the syringe pump, use this option to set a purge volume that ensures all stranded medium is properly expelled.

Select option 6, Purge Volume. Enter a value for the purge volume that ensures at least two strokes of the syringe plunger and press ENTER. The Program Variables screen displays.

Replacement Media Option screen displays. Select option 2, Disable. The Program Variables screen displays.

Note: The replacement media option is not available for use with the Apparatus 3 / 7. The replacement media option must be disabled or the instrument will not operate properly.

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4 Operating the 8000

NOTE

Tabl e 2 2 Program Variables Options (Apparatus 3 / 7)

Option Function

7 Filter Chg (syringe

pump and filter changer)

7 R-M (syringe pump) If your system configuration includes the syringe pump but not the filter changer, select

The replacement media option is not available with this system configuration. Ensure the RM option for the syringe pump is disabled using the hidden key function MENU > 0 > R.

option 7, R-M. The Replacement Media Option screen displays. Select option 2, Disable. The Program Variables screen displays.

Note: The replacement media option is not available for use with the Apparatus 3 / 7. The replacement media option must be disabled or the instrument will not operate properly.

Program Option 2 - Sample Time Points

From the Program Variables screen, select option 2, Sample Time Points. The following screen displays:

SAMPLE TIME POINT: 000:02

ENTER hhh:mm UP TO 999:59 HOURS

PROG #: 1 ROW: 0 VOLUME: 5ml

TIME:01/01/12 09:37:14 ELAPSE:000:00:00

90 8000 Operator’s Manual

This screen accepts sampling times and saves them for execution when the program is run. Time points are requested in order of execution starting with collection row number 0 on the sample tray. The sample time is entered as hours and minutes. Once you enter a time, the row number increments and another sample time point is requested.

Operating the 8000 4

NOTE

The following conventions are used with this screen:

• Review previously entered sample time points by pressing ENTER

to scroll through the entries.

• After all desired sample time points have been entered, enter 000:00

for the last sample time point and press ENTER. If the maximum of ten sample time points are entered, you do not have to enter 000:00 for the last sample time point.

• To add additional sample time points, press ENTER until a new row

time displays. Enter the additional sample time point.

• If more than ten sample time points are needed, use the link program

feature. See “Starting a Program” on page 91.

• To correct an entry, press BACKSPACE to go to the previous

character position and re-enter the correct value.

• If a shorter sample time point than the previous one is entered for a

given row, the row number will not increment. Times must increase from one row to the next.

• To shorten a saved program, enter 000:00 for the first sample time

point you wish to discard. All successive time points are eliminated.

• Press ESC to save all new values and return to the Program Variables

screen.

8000 Operator’s Manual 91

Starting a Program

1 From the Ready screen, press START PROG to start a program or

modify a program already operating.

2 Enter a program number between 1 and 15 and press ENTER. The

Link Program Option screen displays.

***LINK PROGRAM OPTION***

1 RUN SINGLE PROG 2 LINK ANOTHER PROG

4 Operating the 8000

NOTE

TIME: 01/01/12 15:02:30 ELAPSE: 000:00:04

3 To run a single program, select option 1.

To link another program to the one previously selected, select option 2. The following screen displays:

LINK PROG #: (1-15):__

TIME: 01/01/12 15:02:30 ELAPSE: 000:00:04

Enter the program number to link to the current program and press

ENTER.

92 8000 Operator’s Manual

When running two programs, it is necessary to replace the full sample tray with an empty one between programs. Enable the program completion alarm (see “5 PRG COMPL ALARM” on page 51) to alert the analyst when the first program completes and the second program begins.

4 The Select Start Mode screen displays. Options 1 - 3 start the

collection program. Option 4 allows you to change or review the program before beginning.

***SELECT START MODE***

1 START NOW 2 DELAYED START

3 REMOTE START 4 MODIFY PROGRAM

PROG#: 1 R-M: DISABLED VOL: 5 ml

Operating the 8000 4

Following is a description of the Select Start Mode screen options:

Tabl e 2 3 Select Start Mode Options (Apparatus 3 / 7)

Option Function

1 Start Now Select option 1, Start Now. The APP 3/7 Options screen displays.

2 Delayed Start Select option 2, Delayed Start. The APP 3/7 Options screen displays.

3 Remote Start This option is designed for use with the 8000 coupled with non-Agilent

instruments. Contact the Dissolution Systems Service Department for information about this function.

4 Modify Program Select option 4, Modify Program, to modify or review the selected program.

“Creating a Sample Program” on page 61 to review programming options. All

program variable items can be viewed or changed.

Press ESC to return to the Select Start Mode screen.

Selecting option 4 again repeats the modify program operation.

When the program is verified or changed to the correct settings, select the desired start mode (option 1 - 3) to start the program.

APP 3/7 Options Screen

***APP 3/7 OPTIONS***

1 LOAD & RUN APP 3/7 2 RUN WO/ LOADING

3 RUN 8000 ONLY 4 SET APP 3/7

TIME: 01/01/12 08:09:51 ELAPSE: 000:00:00

Following is a description of the APP 3/7 Options screen options:

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4 Operating the 8000

Tabl e 2 4 App 3/7 Options

Option Start Now Function Delayed Start Function

1 Load &

Run APP 3/7

2 Run WO/ Loading

3 Run 8000 Only

Select option 1, Load & Run APP 3/7, to upload all saved parameters to the Apparatus 3 / 7 and start the program.

Select option 2, Run WO/ Loading, to start the program and run the 8000 without loading and overriding the Apparatus 3 / 7 settings.

Select option 3, Run 8000 Only, to immediately start the 8000 only. The 8000 executes the program currently selected.

This option is used when other instrumentation, such as an Apparatus 3 / 7, is connected to the 8000 through the START OUTPUT jack on the rear of the instrument.

Select option 1, Load & Run APP 3/7, to upload all saved parameters to the Apparatus 3 / 7.

Enter the start time in 24-hour format and press ENTER. Enter the start date and press ENTER.

If the delayed start alarm is enabled, enter the duration before the start of the program you would like the alarm to sound. To disable the alarm, see MENU > 0 > C under “Hidden Key Functions” on page 41.

At any time prior to the scheduled start of the program, press ESC to cancel the delayed start.

Select option 2, Run WO/ Loading, to run the 8000 without loading and overriding the Apparatus 3 / 7 settings.

Enter the start time in 24-hour format and press ENTER. Enter the start date and press ENTER.

At any time prior to the scheduled start of the program, press ESC to cancel the delayed start.

Select option 3, Run 8000 Only, to start the 8000 only.

Enter the start time in 24-hour format and press ENTER. Enter the start date and press ENTER. Once the start date and time are reached, the 8000 executes the program currently selected.

At any time prior to the scheduled start of the program, press ESC to cancel the delayed start.

4 Set APP 3/7

Use this option to modify or review the current program parameters. Select option 4 Set APP 3/7 Parameters screen displays. See “Main Menu Option 2 - Set APP 3/7” on page 79 for a description of the Set APP 3/7 Parameters screen options.

, Set APP 3/7. The

94 8000 Operator’s Manual

Manual Sample

Operating the 8000 4

From the Ready screen, press MANUAL SAMPLE to collect a sample from the dissolution apparatus on command without a program. These options are only for manual sampling and have nothing to do with the stored program parameters for purge volume, prime volume, and so on.

When your system configuration includes the peristaltic pump, the following Manual Sampling screen displays:

***MANUAL SAMPLING***

1 START 2 SET ROW # 3 SET VOLUME

4 PRIME TIME 5 PURGE TIME 6 R-M OPTION

ROW 3 VOLUME 5ml PRIME 60 PURGE 60

When your system configuration includes the syringe pump with or without the filter changer, the following Manual Sampling screen displays:

***MANUAL SAMPLING***

1 START 2 SET ROW # 3 SET VOLUME

4 PRIME VOL. 5 PURGE VOL. 6 R-M OPTION

ROW 3 VOLUME 5ml PRIME 10 PURGE 20

Following is a description of the Manual Sampling screen options:

Tabl e 2 5 Manual Sampling Options

Option Function

1 Start Select option 1, Start, to start the manual sampling procedure using the values you

enter for options 2 through 6 on the Manual Sampling screen. The display screen details each step of the manual collection as it takes place.

Note: Select this option last.

2 Set Row # Select option 2, Set Row #. Enter the row number between 0 and 9 where you want

the samples deposited in the sample tray and press ENTER. The Manual Sampling screen displays.

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4 Operating the 8000

Tabl e 2 5 Manual Sampling Options

Option Function

3 Set Volume Select option 3, Set Volume. Enter a sample volume between 0 and 14 mL and press

ENTER. The Manual Sampling screen displays.

Note: The volume can contain a decimal point so that fractional milliliters can be collected. Be sure not to enter a volume larger than the collection tubes can contain.

4 Prime Time (peristaltic pump)

4 Prime Vol (syringe pump)

5 Purge Time (peristaltic pump)

5 Purge Vol (syringe pump)

6 R-M Option Select option 6, R-M Option. The Replacement Media Option screen displays. Select

Select option 4, Prime Time. Enter a value for the priming time and press ENTER. The Manual Sampling screen displays.

Note: The priming time is the interval the peristaltic pump runs forward, drawing medium into the system, before the valves open to deliver samples.

Select option 4, Prime Vol. Enter a value for the priming volume and press ENTER. The Manual Sampling screen displays.

Note: The priming volume is the amount of drawn medium necessary to fill the sampling lines of the entire system.

Select option 5, Purge Time. Enter a value for the purging time and press ENTER. The Manual Sampling screen displays.

Note: The purging time is the interval the peristaltic pump runs in reverse to return the uncollected medium in each line back into the vessel from which it was drawn. Purging also clears the sampling filters of particulate matter that may restrict successive samples.

Select option 5, Purge Vol. Enter a value for the purging volume that ensures at least two strokes of the syringe plunger and press ENTER. The Manual Sampling screen displays.

option 2 to disable media replacement. The Manual Sampling screen displays.

Note: The media replacement option is not available with the Apparatus 3 / 7. Ensure this option is disabled or the instrument will not operate properly.

96 8000 Operator’s Manual

Volumetric Calibration (Peristaltic Pump only)

NOTE

The CAL key on the 8000 can be used only if the peristaltic pump is installed on your system. To calibrate the syringes in the 806 Syringe Pump, see “5 Full Stroke (hidden option)” under “Main Menu Option 3

- Set Syringe Pump” on page 80“.

It is critical that sample volumes are accurately and precisely taken. Calibration of your system ensures this by recording in memory the time required to deliver a nominal 10 mL of medium into the graduated calibration tubes. Refer to “Automatic Calibration” below or “Manual

Calibration” on page 99 for specific operating procedures necessary to

calibrate your 8000.

Ensure the cannula filters are in place during calibration.

Automatic Calibration

Operating the 8000 4

8000 Operator’s Manual 97

To perform automatic calibration, complete the following steps:

1 Ensure the sample cannulas of the dissolution apparatus are

sufficiently submerged in water.

2 Ensure the correct prime and purge times have been entered via the

Main Menu options 4 and 5, Manual Prime Time and Manual Purge Time, respectively.

3 Ensure the supplied autocalibration block is firmly in place and

connected to the rear panel jack labeled AUTO CALIBRATION.

4 From the Ready screen, press CAL. The dispensing arm moves

forward to allow you to check the autocalibration block.

4 Operating the 8000

NOTE

5 The 8000 system monitor displays a reminder to ensure the tubing

and pump are ready. The Calibrate Sampling Volume screen displays.

***CALIBRATE SAMPLING VOLUME***

CHECK PUMP & TUBING. IF AUTO CALIBRATION

INSTALL CALIB FIXTURE BEFORE STARTING!

1 MANUAL 2 AUTOMATIC

6 Select option 2, Automatic. The dispensing arm moves over the

autocalibration block and primes the lines according to the manual prime settings. At the end of the priming cycle, all valves lower, open simultaneously, and begin to fill the autocalibration block. As the 10 mL point is reached in each well, the valve closes and the time value is recorded in memory. When the last valve closes, the pump reverses and purges the lines. The calibration completion alarm, if enabled, sounds when the calibration sequence is finished. Press any key to silence the alarm.

Because of intrinsic variations in tubing diameters, internal valve passages, and pump tubing tensions, some valves must stay open longer than others to deliver their nominal 10 mL calibration volumes. This is normal operation.

98 8000 Operator’s Manual

7 After the dispensing arm moves forward, remove the autocalibration

block.

8 Clean and dry the separate portions of the autocalibration block.

9 Reconnect the top and bottom portions of the autocalibration block

and replace it.

10 Press ESC to return the dispensing arm to the home position.

Manual Calibration

CAUTION

NOTE

Do not use any acidic media to calibrate your 8000. Be sure to rinse and dry the autocalibration block tips after completion.

We suggest calibrating with tap water. Deionized water does not work well with the autocalibration block which utilizes conductivity testing.

To perform the calibration yourself, complete the following steps:

1 Remove the sample tray.

2 Ensure the sample cannulas of the dissolution apparatus are

sufficiently submerged in water.

3 Ensure the correct prime and purge times have been entered via the

Main Menu option 4 and 5, Manual Prime Time and Manual Purge Time, respectively.

4 Press MENU.

5 Select option 7, Manual Operation.

6 Select option 4, Goto Rows.

7 Enter 4 to move the dispensing arm to row 4.

8 Remove the media rinse reservoir.

9 Press H to return to the home position.

10 Press ESC three times to return to the Ready screen.

11 From Ready screen, press CAL. The dispensing arm moves forward

to allow you to check the autocalibration block.

12 Remove the top portion of the autocalibration block and place it on

its side to prevent damage.

13 Place clean, empty 10 mL calibration tubes in the bottom portion of

the autocalibration block.

14 Select option 1, Manual. The dispensing arm moves over the

autocalibration block and primes the lines according to the manual prime settings.

Operating the 8000 4

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4 Operating the 8000

NOTE

15 Upon completion of the priming cycle, the following screen displays:

PRESS <OPEN VALVES> TO START

PRESS AGAIN WHEN 10 ML IS REACHED

MANUAL CALIBRATION OF VALVE # 1

TIME: 01/01/12 21:56:40 ELAPSE: 000:00:00

16 Press OPEN VALVES. The first calibration tube begins filling.

17 Observe the first calibration tube and press OPEN VALVES again

when 10 mL is reached.

18 Repeat Steps 17 and 18 for the remaining calibration tubes. When the

last valve is calibrated, the pump reverses and the lines are purged. The calibration completion alarm, if enabled, sounds when the calibration sequence is finished.

19 Press any key to silence the alarm.

20 After the dispensing arm moves forward, remove and clean the

calibration tubes.

21 Reconnect the top and bottom portions of the autocalibration block.

22 Press ESC.

100 8000 Operator’s Manual

If you press ESC before all the valves are calibrated, you cancel the calibration and return to the previously saved values for each valve. None of the new calibration results are saved. The calibration must be allowed to finish before the new calibration values are saved.

23 Press MENU.

24 Select option 7, Manual Operation.

25 Select option 4, Goto Rows.

26 Enter 4 to move the dispensing arm to row 4.

27 Replace the media rinse reservoir in front of the autocalibration

block.

28 Press H to return to the home position.

29 Press ESC three times to return to the Ready screen.

Agilent Technologies 8000 Operator's Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Contents

Figures

Tables

Electrical Hazards

Warning

CAUTION

NOTE

Information Symbols

8000 with Peristaltic Pump

Conventions Used in this Manual

Serial Number Format

Unpacking of 8000 and Components

Unpacking Your 8000

Unpacking / Setup of 8000 & Peristaltic Pump

Unpacking / Setup of 8000 & Syringe Pump

Unpacking / Setup of 8000, Syringe Pump, and Filter Changer

Setup of 8000 and Components

Installing 8000 Full Flow Filters

Power and Network Connections

Installing the Sample Tray

Installing the Media Rinse Reservoir

Installing and Connecting the Autocalibration Block (Peristaltic Pump only)

Installing the RM Module

Loading Filters (Filter Changer only)

Changing Filter Types (Filter Changer Only)

Hidden Key Functions

Operating the 8000 with Apparatus 1 / 2

8000 Keypad Options

8000 Menu Options - Apparatus 1 / 2

Creating a Sample Program

Starting a 8000 Program

Operating the 8000 with Apparatus 3 / 7

8000 Keypad Options

8000 with Apparatus 3 / 7 - Menu Options

Creating a Sample Program

Starting a Program

Manual Sample

Volumetric Calibration (Peristaltic Pump only)

Automatic Calibration

Manual Calibration