Agilent Technologies 2200 User Manual
User Manual
Agilent 2200 TapeStation
System
Agilent Technologies
Notices
CAUTION
WARNING
© Agilent Technologies, Inc. 2011-2014,
2015
No part of this manual may be reproduced
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States and international copyright laws.
Manual Part Number
G2964-90003 Rev. C
Edition
09/2015
Printed in Germany
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Safety Notices
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly performed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated conditions are fully understood and
met.
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indicated conditions are fully understood and met.
Agilent 2200 TapeStation System - User Manual
Contents
Contents
1 Introduction to the 2200 TapeStation System 5
Overview of the System 6
2 Specifications 11
Technical Specifications 12
ScreenTape Specifications 14
3 Installing the System 23
Unpacking the System 24
Contents of the 2200 TapeStation System 26
Installing the System 30
4 Using the 2200 TapeStation System 33
Intended Use of the 2200 TapeStation System 35
Performance Limitations of Use 36
Additional Components Required by the User 37
Operating Procedure 38
How to prepare your samples 50
Good Measurement Practices for Analysing DNA on the Agilent 2200
Ta p e S t a t i o n 52
DNA Sample Preparation 53
RNA Sample Preparation 61
Protein Sample Preparation 67
5 Maintenance 71
General Information 72
Changing the Needle 73
Preventative Maintenance Interval 76
Tap eS ta ti on Te st Tap e 78
Agilent 2200 TapeStation System - User Manual 3
Contents
6 Appendix 83
Limited Use Label License 84
Sound Emission 85
Waste Electrical and Electronic Equipment Directive 86
Technical Service 87
4 Agilent 2200 TapeStation System - User Manual
Agilent 2200 TapeStation System - User Manual
1 Introduction to the 2200 TapeStation System
Overview of the System 6
ScreenTape Device Architecture 7
Agilent 2200 TapeStation Instrument Components 8
This chapter gives an introduction to the system
Agilent Technologies
5
1 Introduction to the 2200 TapeStation System
Overview of the System
Overview of the System
The Agilent 2200 TapeStation system is a revolutionary automated
platform for simpler, faster and more reliable electrophoresis. It is made
up of three elements: a consumable tape (ScreenTape device), an
instrument (the 2200 TapeStation instrument), and an Agilent Software
package (2200 TapeStation Controller Software, and Agilent TapeStation
Analysis Software). The system is very straightforward to use, simply
place the sample tubes and ScreenTape device in to the 2200 TapeStation
instrument and let it load, separate, image, analyze, and present the
results.
Place ScreenTape device and some tips in the 2200 TapeStation
instrument.
Place your samples in the 2200 TapeStation instrument and click
Start on the 2200 TapeStation Controller Software.
View your analyzed results in around 1 min per sample.
This User Manual guides the operation of all ScreenTape assays, the 2200
TapeStation instrument, and software for the analysis of DNA, RNA, and
protein samples. The contents of the ScreenTape system are detailed
below.
Information pertaining to the 2200 TapeStation system can be found in:
• Technical Specification
• Agilent 2200 TapeStation Instrument Components
• Installing the System
6 Agilent 2200 TapeStation System - User Manual
Information pertaining to sample and ScreenTape device requirements can
be found in:
• ScreenTape Device Architecture
• Operating Procedure
ScreenTape Device Architecture
Barcode: The unique barcode tracks lane usage within each ScreenTape device and
allows traceability of results.
Buffer chamber: The buffer chamber is located at the top of the channel and contains
optimised buffers for the effective separation of nucleic acid fragments or
proteins.
Electrodes: The integrated electrodes apply a current across the ScreenTape device
and eliminate the need for any additional electrophoresis equipment.
Gel: The gel contained within the ScreenTape device has been developed
specifically to resolve nucleic acids or proteins.
Introduction to the 2200 TapeStation System
Overview of the System
1
ScreenTape device
product details:
The information is unique to each ScreenTape device. This includes:
ScreenTape assay type, product expiry date and a unique serial number.
Figure 1 Example: P200 ScreenTape device
Agilent 2200 TapeStation System - User Manual 7
1 Introduction to the 2200 TapeStation System
Overview of the System
Agilent 2200 TapeStation Instrument Components
Lid: The 2200 TapeStation instrument lid must be closed each time the 2200
TapeStation Controller Software is initialized, and whilst the instrument is
in operation.
LED: The LED will illuminate once the instrument is on. When the LED is
flashing slowly, the instrument is in use and the lid should not be opened,
rapid flashing indicates that the 2200 TapeStation instrument requires
attention.
Sample Block: There are 2 sample blocks provided that can either hold 0.2 mL sample
tube strips or a 96-well plate.
Tip Holder: The tip holder can accommodate up to 16 loading tips at any one time.
ScreenTape device: The ScreenTape device must be placed into the holder with the barcode
towards the front of the instrument, facing towards the right.
USB Socket: The USB connector is inserted into the USB socket to link the laptop to the
2200 TapeStation instrument.
Power-cable socket: The power cable must be connected to the 2200 TapeStation instrument
and the relevant mains electricity outlet.
8 Agilent 2200 TapeStation System - User Manual
Introduction to the 2200 TapeStation System
A^Y
HXgZZcIVeZYZk^XZ
I^e]daYZg
A:9
HVbeaZWadX`
USB socket
On/off switch
Power-cable socket
Overview of the System
1
Agilent 2200 TapeStation System - User Manual 9
Figure 2 2200 TapeStation instrument (front view)
Figure 3 2200 TapeStation instrument (back view)
1 Introduction to the 2200 TapeStation System
Overview of the System
10 Agilent 2200 TapeStation System - User Manual
Agilent 2200 TapeStation System - User Manual
2 Specifications
Technical Specifications 12
ScreenTape Specifications 14
This chapter provides information on specifications.
Agilent Technologies
11
2 Specifications
Technical Specifications
Technical Specifications
2200 TapeStation instrument
Input voltage: 12 V DC
Power consumption: 40 W (VA)
Current: 3 A
Interface: USB cable (PC comms.)
Instrument Housing: UL94/VO rated flame retardant cast polyurethane
Dimensions: 400x 310x 310 mm
Weight: 12.5 kg
Power supply
Input voltage: 100 – 240 V AC
Input frequency: 50 – 60 Hz
Phase: 1
Current: 0.45 – 1.1 A
Environmental conditions
Optimal operating
temperature
Instrument operating
temperature
1
Instrument operating temperature may be higher than ambient lab temperature, especially after
prolonged use.
1
20 °C (68 °F).
12 – 37 °C (54 – 99 °F) for D1000 ScreenTape assay
17 – 37 °C (63 – 99 °F) for High Sensitivity D1000 ScreenTape assay
15 – 30 °C (59 – 86 °F) for Genomic DNA, D5000 and High Sensitivity
D5000 ScreenTape assays
14 – 30 °C (57 – 86 °F) for RNA and High Sensitivity RNA ScreenTape
assays
10 – 33 °C (50 – 91 °F) for P200 ScreenTape assay
12 Agilent 2200 TapeStation System - User Manual
Specifications
NOTE
Technical Specifications
If the instrument is out of the recommended temperature range for the ScreenTape assay
selected, the following error message will appear in the software:
• If the quoted current temperature is above the specified range, please move the
system out of direct sunlight and away from any windows. Check that any air
conditioning is functioning.
2
• If the quoted current temperature is below the specified range, please allow the
instrument to equilibrate to the ambient temperature, and avoid using in a cooled
area.
Agilent 2200 TapeStation System - User Manual 13
2 Specifications
ScreenTape Specifications
ScreenTape Specifications
Specification (D1000 ScreenTape Assay)
Analytical Specifications D1000 ScreenTape assay and reagents
Sizing Range 35 – 1000 bp
Typical Resolution 35 – 300 bp: 15 %
Sensitivity
Sizing Precision 5 % CV
Sizing Accuracy
Quantitative Precision 0.1 – 1 ng/µL: 15 % CV
Quantitative Accuracy
Quantitative Range 0.1 – 50 ng/µL
1
2
3
300 – 1000 bp: 10 %
0.1 ng/µL
±10 %
1 – 50 ng/µL: 10 % CV
±20 %
Maximum sample buffer strength 20 mM KCl,
60 mM Phosphate Buffer,
60 mM Guanidine-HCl,
240 mM NaCl,
60 mM Acetate
Physical Specifications
Analysis Time 16 samples: <20 min
96 samples: ≈100 min
Samples per consumable 16
Sample volume required 1 µL
Kit stability 4 months
Kit size 112 samples
1
signal-to-noise >3 (single peak)
2
Sizing Accuracy for electronic ladder: ±20%
3
Measured against 2100 Bioanalyzer system
14 Agilent 2200 TapeStation System - User Manual
Specifications
ScreenTape Specifications
Specification (High Sensitivity D1000 ScreenTape Assay)
Analytical Specifications High Sensitivity D1000 ScreenTape assay and reagents
Sizing Range 35 – 1000 bp
Typical Resolution 35 – 300 bp: 15 %
300 – 1000 bp: 10 %
Sensitivity
1
Sizing Precision 5 % CV
Sizing Accuracy
2
Quantitative Precision 15 % CV
Quantitative Accuracy
3
Quantitative Range 10 – 1000 pg/µL
Maximum sample buffer strength 7 mM KCl,
5pg/µL
±10 %
±20 %
20 mM Phosphate Buffer,
20 mM Guanidine-HCl,
80 mM NaCl,
20 mM Acetate
2
Physical Specifications
Analysis Time 16 samples: <20 min
96 samples: ≈100 min
Samples per consumable 16
Sample volume required 2 µL
Kit stability 4 months
Kit size 112 samples
1
signal-to-noise >3 (single peak)
2
Sizing Accuracy for electronic ladder: ±20%
3
Measured against 2100 Bioanalyzer system
Agilent 2200 TapeStation System - User Manual 15
2 Specifications
ScreenTape Specifications
Specification (D5000 ScreenTape Assay)
Analytical Specifications D5000 ScreenTape assay and reagents
Sizing Range 100 – 5000 bp
Typical Resolution 400 – 5000 bp: 15 %
Sensitivity
Sizing Precision
Sizing Accuracy
1
2
2,3
Quantification Precision 0.1 – 1 ng/µL: 15 % CV
Quantification Accuracy
4
Quantification Range 0.1 – 50 ng/µL
Maximum Sample Buffer Strength 250 mM KCl,
Physical Specifications
Multiple ScreenTape (96 well)
compatibility
Analysis Time 16 samples: <25 min
Samples per consumable 1 ladder + 15 samples
Sample volume required 1 µL
Kit stability 4 months
Kit size 112 samples/box
0.1 ng/µL
5% CV
±10 %
1 – 50 ng/µL: 10 % CV
±20 %
250 mM Tris-HCl,
125 mM NaCl,
50 mM Acetate,
25 mM MgCl
,
2
25 mM BSA,
25 mM Guanidine-HCl
Not compatible
1
signal-to-noise >3 (single peak)
2
Determined using the D5000 Ladder as sample
3
Accuracy for run using single ScreenTape device
4
Measured against 2100 Bioanalyzer system
16 Agilent 2200 TapeStation System - User Manual
ScreenTape Specifications
Specification (HS D5000 ScreenTape Assay)
Analytical Specifications D5000 ScreenTape assay and reagents
Sizing Range 100 – 5000 bp
Typical Resolution 400 – 5000 bp: 15 %
Sensitivity
Sizing Precision
Sizing Accuracy
1
2
3
3
Quantification Precision 15 % CV
Quantification Accuracy
4
Quantification Range 10 – 1000 pg/µL
Maximum Sample Buffer Strength 25 mM KCl,
5pg/µL
10 % CV
±15 %
±25 %
25 mM Tris-HCl,
12.5 mM NaCl,
5 mM Acetate,
2.5 mM MgCl
,
2
2.5 mM BSA,
2.5 mM Guanidine-HCl
Specifications
2
Physical Specifications
Multiple ScreenTape (96 well)
Not compatible
compatibility
Analysis Time 16 samples: <25 min
Samples per consumable 1 ladder + 15 samples
Sample volume required 2 µL
Kit stability 4 months
Kit size 112 samples/box
1
signal-to-noise >3 (single peak)
2
Determined using the High Sensitivity D5000 Ladder as sample
3
Accuracy for run using single ScreenTape device
4
Measured against 2100 Bioanalyzer system
Agilent 2200 TapeStation System - User Manual 17
2 Specifications
ScreenTape Specifications
Specification (Genomic DNA ScreenTape Assay)
Analytical Specification Genomic DNA ScreenTape assay and reagents
Sizing Range 200 bp to > 60000 bp
Sensitivity 0.5 ng/µL
Sizing Precision
Sizing Accuracy
Quantitative Precision
Quantitative Accuracy
1
1
2
3
Linear Concentration Range 10 – 100 ng/µL
DIN functional range 5 – 300 ng/µL
Physical Specification
Analysis Time 16 samples: < 25 min
Samples per consumable 1 ladder + 15 samples
200 – 15000 bp: 15 % CV
200 – 15000 bp: ±15 %
15 % CV
±20 %
96 samples: < 150 min
Sample Volume Required 1 µL
Shelf Life 4 months
Box/Kit size 112 samples/box
1
Determined using the Genomic DNA Ladder as sample
2
Average result from various genomic DNA sample types
18 Agilent 2200 TapeStation System - User Manual
ScreenTape Specifications
Specification (RNA ScreenTape Assay)
Analytical Specification RNA ScreenTape assay and reagents
Quality Score
Quantitative Range 25 – 500 ng/µL
Quantitative Precision (%CV)
1
Quantitative Accuracy 20 %
Sizing Range 100 – 6000 nt
Sensitivity
RIN
2
e
functional range
Analysis Type Eukaryotic or Prokaryotic Total RNA QC
Maximum sample buffer strength 200 mM Tris,
Physical Specifications
e
RIN
5%
5ng/µL
25 – 500 ng/µL
20 mM EDTA, or
50 mM NaCl
Specifications
2
Analysis Time 16 samples < 16 min
96 samples < 100 min
Samples per consumable 16
Sample volume required (µL) 1
Kit Stability 4 months
Kit Size 112 samples
For total RNA samples
1
Within a ScreenTape
2
Signal/noise >3 in water and TE
device
Agilent 2200 TapeStation System - User Manual 19
2 Specifications
ScreenTape Specifications
Specification (High Sensitivity RNA ScreenTape Assay)
Analytical Specification HS RNA ScreenTape assay and reagents
Quality Score
Quantitative Range 500 – 10000 pg/µL
Quantitative Precision (%CV)
1
Quantitative Accuracy 30 %
Sizing Range 100 – 6000 nt
Sensitivity
RIN
2
e
functional range
Analysis Type Eukaryotic or Prokaryotic Total RNA QC
Maximum sample buffer strength 10 mM Tris
Physical Specifications
e
RIN
10 %
100 pg/µL
1000 – 25000 pg/µL
1mM EDTA
Analysis Time 16 samples < 30 min
96 samples < 180 min
Samples per consumable 16
Sample volume required (µL) 2
Kit Stability 4 months
Kit Size 112 samples
For total RNA samples
1
Within a ScreenTape
2
Signal/noise >3 in water and TE
device
20 Agilent 2200 TapeStation System - User Manual
Specifications
ScreenTape Specifications
Specification (P200 ScreenTape Assay)
Analytical Specification P200 ScreenTape assay and reagents
Sizing range 10 – 200 kDa
Resolution
Typical Sizing Accuracy ±10 % (CAII, Lysozyme, beta lactoglobulin)
Sizing Precision 3 % CV
Quantitative Range/precision 100 – 1000 ng/µL for IgG; 15 % CV
Qualitative Range 5 – 5000 ng/µL BSA, Lysozyme; 12.5 – 5000 ng/µL IgG
Sensitivity
Physical Specification
Sample volume needed 2 µL
Analysis Time 16 samples: <15 min
Samples/consumable 16
1
2
15 %
5 ng/µL Lysozyme; 12.5 ng/µL IgG
2
Kit Size 112 Samples
Kit Stability 4 months
1
determined using P200 Ladder as sample
2
signal :noise ratio > 3
Agilent 2200 TapeStation System - User Manual 21
2 Specifications
ScreenTape Specifications
22 Agilent 2200 TapeStation System - User Manual
Agilent 2200 TapeStation System - User Manual
3 Installing the System
Unpacking the System 24
Contents of the 2200 TapeStation System 26
Installing the System 30
Software Installation 30
Agilent 2200 TapeStation System Set Up 31
This chapter gives information about how to install the system.
Agilent Technologies
23
3 Installing the System
CAUTION
CAUTION
WARNING
Unpacking the System
Unpacking the System
Unpacking the Agilent 2200 TapeStation Instrument
Prerequisites The installation and familiarization service is bundled with instrument sale, and will be
conducted by an Agilent engineer.
Instrument and software familiarization should be completed before use.
Do not attempt to use the 2200 TapeStation instrument until you have read the
accompanying Site and Safety Manual.
Condensation within the instrument
Condensation will damage the system electronics.
➔ If your instrument was shipped in cold weather, leave it in its box and allow it to
warm slowly to room temperature to avoid condensation.
24 Agilent 2200 TapeStation System - User Manual
"Defective on arrival" problems
If there are signs of damage, please do not attempt to install the instrument. Inspection
by Agilent is required to evaluate if the instrument is in good working condition.
➔ Notify your local Agilent Representative and the Technical support channel.
➔ An Agilent service representative will inspect the instrument at your site and
initiate appropriate actions.
Personal injury
The TapeStation instrument is heavy.
➔ Enlist the aid of a co-worker to share the lifting load to avoid personal injury.
1 Remove the 2200 TapeStation instrument from the packaging and place
NOTE
on a clean, dry, flat surface.
2 Allow the 2200 TapeStation instrument to acclimatize to the ambient
temperature of the operating environment.
3 Remove the label covering the ScreenTape device holder, as shown in
the image below.
Figure 4 Remove before use
Delivery Checklist
Ensure all parts and materials have been delivered with your system. The
delivery checklist is shown below.
Installing the System
Unpacking the System
3
Please report any missing or damaged parts to your local Agilent
Technologies sales and service office.
Please note that ScreenTape devices and reagent kits are not provided with instrument
delivery and must be purchased separately.
Agilent 2200 TapeStation System - User Manual 25
3 Installing the System
Contents of the 2200 TapeStation System
Contents of the 2200 TapeStation System
The Agilent 2200 TapeStation System
Ta b le 1 The Agilent 2200 TapeStation System (G2964AA, G2965AA)
Product Volume Properties
Agilent 2200
TapeStation
instrument
2200 TapeStation
Software Setup Disc
Laptop 1x Laptop Instrument Control Laptop
USB Cables/Power
supply units
Sample Block 1 x 0.2 mL strip
Tip Holder 2x A removable cartridge for pipette tips placed in the 2200 TapeStation
Loading tips 1x 384tips Pipette tips to use in the 2200 TapeStation instrument
2200 TapeStation
instrument compatible 0.2 mL
tube strips and lids
96-well plates pack of 10
96-well plate foil seal pack of 100
1x Instrument for loading, electrophoresis, imaging, and analyzing:
• 2200 TapeStation system (G2964AA) or
• 2200 TapeStation Nucleic Acid system (G2965AA)
1x CD The software is required to drive the 2200 TapeStation instrument and visualize
the ScreenTape device analysis
1x USB cable
2x power cords
and
1 x 96-well plate
1x box of 120
tubes and caps
1x USB cable to connect the laptop to the 2200 TapeStation instrument
1x Power supply unit for the laptop
1x Power supply unit for the 2200 TapeStation instrument
A removable sample block for the correct loading of samples within the
2200 TapeStation instrument
instrument
Tube strips for placing samples mixed with loading buffer into the
2200 TapeStation instrument
Loading tip transfer
tool (optional)
IKA Vortexer and
associated PCR plate
(optional)
Guides Site Safety guide and Quick Guides (G2964AA - Protein, DNA and RNA;
1x
1x Vortexer for mixing samples and reagents
G2965AA - DNA and RNA)
26 Agilent 2200 TapeStation System - User Manual
Installing the System
Contents of the 2200 TapeStation System
ScreenTape Products
Kit Components (High Sensitivity D1000 ScreenTape Assay)
Part Number Name Color Amount
5067-5584 High Sensitivity D1000 ScreenTape 7 ScreenTape devices
3
5067-5585 High Sensitivity D1000 Reagents
• High Sensitivity D1000 Ladder
• High Sensitivity D1000 Sample Buffer
5067-5587 High Sensitivity D1000 Ladder 1 vial
2 vials
20 µL
300 µL
20 µL
Kit Components (D1000 ScreenTape Assay)
Part Number Name Color Amount
5067-5582 D1000 ScreenTape 7 ScreenTape devices
5067-5583 D1000 Reagents
• D1000 Ladder
• D1000 Sample Buffer
5067-5586 D1000 Ladder 1 vial, 10 µL
2 vials
10 µL
400 µL
Agilent 2200 TapeStation System - User Manual 27
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