YSI pHotoFlex Operating Manual
OPERATIONS MANUAL
STD
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®
pHotoFlex
STD
Handheld colorimeter
pHotoFlex®STD
For the most recent version of the manual, please visit www.ysi.com.
Contact YSI
1725 Brannum Lane
Yellow Springs, OH 45387 USA
Tel: +1 937-767-7241
800-765-4974
Email: environmental@ysi.com
Internet: www.ysi.com
Copyright © 2013 Xylem Inc.
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pHotoFlex®STD Contents
Contents
1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 General features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.3 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.4 Socket field . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
1.5 LabStation (optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2 Safety instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.1 Safety information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.1.1 Safety information in the operating manual . . . . 11
2.1.2 Safety signs on the meter . . . . . . . . . . . . . . 11
2.1.3 Further documents providing safety information 11
2.2 Safe operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.2.1 Authorized use . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.2.2 Requirements for safe operation . . . . . . . . . . . . 12
2.2.3 Unauthorized use . . . . . . . . . . . . . . . . . . . . . . . 12
3 Commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.1 Scope of delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.2 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.2.1 General information . . . . . . . . . . . . . . . . . . . . . . 13
3.2.2 Inserting/exchanging the batteries . . . . . . . . . . 14
3.3 Initial commissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.1 Switching on the meter . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.2 Inserting a cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4.3 General operating principles . . . . . . . . . . . . . . . . . . . . . 20
4.3.1 Operating modes . . . . . . . . . . . . . . . . . . . . . . . . 20
4.3.2 Navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.3.3 Example 1 on navigation: setting the language 22
4.3.4 Example 2 on navigation: setting the date and
time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.3.5 Menu overview . . . . . . . . . . . . . . . . . . . . . . . . . 26
4.4 System settings (System menu) . . . . . . . . . . . . . . . . . . 28
4.4.1 Measured value memory . . . . . . . . . . . . . . . . . . 29
4.4.2 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
4.4.3 Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
4.4.4 Date/time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
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Contents pHotoFlex®STD
4.5 Photometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
4.5.1 General information . . . . . . . . . . . . . . . . . . . . . . 33
4.5.2 Settings for photometric measurements . 34
4.5.3 Measuring the concentration . . . . . . . . . . . . . . . 35
4.5.4 Blank value (reagent blank value) . . . . . . . . . . . 37
4.5.5 Standard adjustment (user calibration) . . . . . . . 39
4.5.6 Absorbance / % Transmission, measuring . . . . 41
4.5.7 Zero adjustment . . . . . . . . . . . . . . . . . . . . . . . . . 42
4.5.8 Programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
4.5.9 Analysis timer . . . . . . . . . . . . . . . . . . . . . . . . . . 45
4.5.10 Timer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
4.5.11 Measuring diluted samples . . . . . . . . . . . . . . . . 46
4.6 Data storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
4.6.1 Storing measurement datasets . . . . . . . . . . . . . 48
4.6.2 Filtering measurement datasets . . . . . . . . . . . . 49
4.6.3 Displaying measurement datasets . . . . . . . . . . . 50
4.6.4 Download the measurement datasets to the
RS232 interface . . . . . . . . . . . . . . . . . . . . . . . . . 50
4.6.5 Erasing stored measurement datasets . . . . . . . 51
4.7 Transmitting data (RS 232 interface) . . . . . . . . . . . . . . . 51
4.7.1 Connecting a PC . . . . . . . . . . . . . . . . . . . . . . . . 51
4.7.2 Configuring the RS232 interface . . . . . . . . . . . . 52
4.7.3 Selecting the output format of datasets . . . . . . . 52
4.7.4 Transmitting data . . . . . . . . . . . . . . . . . . . . . . . . 54
4.8 Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
4.8.1 Resetting the system settings . . . . . . . . . . . . . . 55
4.8.2 Resetting the photometry settings . . . . . . . . . . . 55
4.9 Meter information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
4.10 Software update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
4.11 Administrating user-defined methods . . . . . . . . . . . . . . . 57
4.11.1 Options for method administration . . . . . . . . . . . 57
4.11.2 Administrating user-defined programs with a
terminal program . . . . . . . . . . . . . . . . . . . . . . . . 58
5 Maintenance, cleaning, disposal . . . . . . . . . . . . . . . . . 61
5.1 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
5.1.1 Inserting/exchanging the batteries . . . . . . . . . . . 61
5.1.2 Retrofitting the rechargeable battery . . . . . . . . . 62
5.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
5.2.1 Cleaning the cell shaft . . . . . . . . . . . . . . . . . . . . 63
5.2.2 Cleaning the cells . . . . . . . . . . . . . . . . . . . . . . . 64
5.3 Packing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
5.4 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
6 What to do if... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .65
6.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
6.2 Photometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
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pHotoFlex®STD Contents
7 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
7.1 General data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
7.1.1 pHotoFlex
7.2 Photometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
®
STD . . . . . . . . . . . . . . . . . . . . . . . . 67
8 Accessories and options . . . . . . . . . . . . . . . . . . . . . . . 69
8.1 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
8.1.1 Meter accessories . . . . . . . . . . . . . . . . . . . . . . . 69
8.1.2 Connection cable: . . . . . . . . . . . . . . . . . . . . . . . 69
8.2 pHotoFlex
®
STDpHotoFlex®STDBar code reader . . . . 69
9 Lists . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
10 Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
11 Firmware update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
12 Contact Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
12.1 Ordering & Technical Support . . . . . . . . . . . . . . . . . . . . 77
12.2 Service Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
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Contents pHotoFlex®STD
6
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pHotoFlex®STD Overview
STD
1
2
4
3
1Overview
1.1 General features
The compact pHotoFlex®STD handheld precision meter enables you
to carry out the following measurements quickly and reliably:
Concentration measurements (colorimetric measurements)
Absorbance measurements
Transmission measurements
The pHotoFlex
®
STD handheld meter provides the maximum degree of
operating comfort, reliability and measuring certainty for all applications.
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1 Keypad
2 Display
3 Cell shaft
(folded out for a 16 mm cell to be inserted)
4 Socket field
7
Overview pHotoFlex®STD
.
.
PRT
1.2 Keypad
Key functions
Select the measured variable <M>:
– Concentration
– Absorbance
– % Transmission
Open the menu for adjustment measurements
(blank values, zero adjustment)
<CAL/ZERO>
Select a program for concentration measurement
<PROG>
Open menus / confirm entries /
start measurement
<START/ENTER>
Call up the Configuration menu
(all settings are made here)
<MENU>
Switch over between available citation forms
<FORM>
Switch over between available units <UNIT>
Switch the meter on or off
<ON/OFF>
Output display contents to RS232 interface
<PRT>
8
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pHotoFlex®STD Overview
Measured parameter
Measured value (with unit)
Program and citation form
Diameter of the cell and measuring range
Status line with date and time
Photometry / Concentration
1.29
mg/l
83: A6/25 MC NH4
-N
16 mm 0.20 - 8.00 mg/l
01.12.11 15:12
12
Open the Store menu: <STO>
Quick storing: 2 x <STO>
Highlight menu items or selection
Set values
<>,<>
Switch to the next higher menu level /
cancel input
<ESC>
Keys with an additional number printed on are assigned doubly.
This enables to directly enter numbers in special menus. Thus, you
can, for example, conveniently enter the date and time via the number
keys.
Example
1.3 Display
The graphic display shows all information of the current measurement
in the measured value display. The illumination enables to read the display even in the darkness.
1.4 Socket field
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9
Overview pHotoFlex®STD
Identifying the
connectors
1 Contacts for operation on the LabStation
2 RS232 serial interface
1.5 LabStation (optional)
The LabStation, which is available as an accessory, enables you to use
the pHotoFlex
erating manual).
Laboratory operation with the LabStation enables the following additional functions:
With photometric measurements, the zero measurement is retained
even after switching the pHotoFlex
You can connect a bar code reader for the simplified calling up of
programs
The LSdata software included serves to easily enter user-defined
programs
Operation with power pack and rechargeable battery (included in the
scope of delivery of the LabStation). The rechargeable battery in the
pHotoFlex
placed in the LabStation.
®
STD conveniently in the laboratory (see LabStation op-
®
STD off and on again
®
STD is automatically charged as soon as the meter is
10
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pHotoFlex®STD Safety instructions
2 Safety instructions
2.1 Safety information
2.1.1 Safety information in the operating manual
This operating manual provides important information on the safe operation of the meter. Read this operating manual thoroughly and make
yourself familiar with the meter before putting it into operation or working with it. The operating manual must be kept in the vicinity of the
meter so you can always find the information you need.
Important safety instructions are highlighted in this operating manual.
They are indicated by the warning symbol (triangle) in the left column.
The signal word (e.g. "Caution") indicates the level of danger:
CAUTION
indicates a possibly dangerous situation that can lead to slight
(reversible) injury if the safety instruction is not followed.
NOTE
indicates a possibly dangerous situation where goods might be
damaged if the actions mentioned are not taken.
2.1.2 Safety signs on the meter
Note all labels, information signs and safety symbols on the meter and
in the battery compartment. A warning symbol (triangle) without text
refers to safety information in this operating manual.
2.1.3 Further documents providing safety information
Observe the safety datasheets of the test reagents when working with
photometric test sets.
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Safety instructions pHotoFlex®STD
2.2 Safe operation
CAUTION
Danger of eye damage by visible and invisible LED radiation. In
the cell shaft there are light emitting diodes (LED) of the 1M class.
Do not look at the radiation using optical instruments. With
normal, authorized use there is no hazard.
2.2.1 Authorized use
This meter is authorized exclusively for the following measurements:
Analysis of substances in water and aqueous solutions
using round cells
Concentration measurement
Absorbance and transmission measurement
The fields of application are mobile use and use in the laboratory. Only
the operation and running of the meter according to the instructions and
technical specifications given in this operating manual is authorized
(see chapter 7 T
ized.
ECHNICAL DATA). Any other use is considered unauthor-
2.2.2 Requirements for safe operation
Note the following points for safe operation:
The meter may only be operated according to the authorized use
specified above.
The meter may only be supplied with power by the energy sources
mentioned in this operating manual.
The meter may only be operated under the environmental conditions
mentioned in this operating manual.
The meter may only be opened if this is explicitly described in this
operating manual (example: Inserting the batteries).
2.2.3 Unauthorized use
The meter must not be put into operation if:
it is visibly damaged (e.g. after being transported)
it was stored under adverse conditions for a lengthy period of time
(storing conditions, see chapter 7 T
ECHNICAL DATA).
12
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pHotoFlex®STD Commissioning
3 Commissioning
3.1 Scope of delivery
Handheld meter pHotoFlex®STD
4 batteries 1.5 V type AA
1 empty cell 16 mm
2 empty cells 28 mm
Microfiber cloth to clean the meter
Quick start guide English
Key overview / Program list
CD-ROM with
– Detailed operating manual
– Photometry analysis manual with analysis specifications
– Software to program user-defined methods
Automatic switchoff
function
Optional: LabStation with LSdata PC software, rechargeable battery
and universal power pack
The optional parts of the scope of delivery are available as accessories
(see section 8.1).
3.2 Power supply
3.2.1 General information
The meter is supplied with power by 4 batteries 1.5 V type AA (see
section 3.2.2).
When you are using the LabStation, you can also supply the meter with
power with a rechargeable battery. The rechargeable battery is included in the scope of delivery of the LabStation and is inserted in the battery compartment instead of the batteries (see LabStation operating
manual).
The meter has an automatic switch-off function in order to save the batteries or rechargeable battery (see section 4.4).
Display illumination During operation with the batteries or rechargeable battery the meter
automatically switches off the display illumination if no key is pressed
for 30 seconds. The illumination is switched on again with the next keystroke. The display illumination can also be switched off completely
(see section 4.4.2).
The LoBat display indicator appears when the batteries or rechargeable battery are nearly discharged.
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Commissioning pHotoFlex®STD
1
2
3.2.2 Inserting/exchanging the batteries
NOTE
Make sure that the poles of the batteries are positioned correctly.
The ± signs on the batteries must correspond to the ± signs in the battery compartment.
14
1 Open the battery compartment:
– Unscrew the two screws (1) on the underside of the meter,
– Remove the lid of the battery compartment (2).
2 If necessary, take four old batteries out of the battery compart-
ment.
3 Insert four batteries (3) in the battery compartment.
4 Close the battery compartment and fix it with the screws.
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pHotoFlex®STD Commissioning
3.3 Initial commissioning
Perform the following activities:
For
– Battery operation: Insert the batteries (see section 3.2.2)
– Operation with LabStation and rechargeable battery: Insert the
rechargeable battery, connect the LabStation and insert the
meter in the LabStation (see LabStation operating manual)
Switch on the meter (see section 4.1)
Set the language as necessary (see section 4.3.3)
Set the date and time as necessary (see section 4.3.4)
When you set the language, date and time according to the mentioned
sections of this operating manual you will quickly become familiar with
the simple operation of the pHotoFlex
®
STD.
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15
Commissioning pHotoFlex®STD
16
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pHotoFlex®STD Operation
Photometry
i pHotoFlex V 1.70
Start
Photometry \ Concentration
iSelect program
with <PROG>
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4 Operation
4.1 Switching on the meter
Switching on Press the <ON/OFF> key.
The Start menu appears for 30 seconds. The status line indicates the
meter designation and the version number of the software.
After a few seconds the meter automatically switches to the measured
parameter used last.
Automatic switchoff
Display illumination
with battery-powered
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Using <M>, switch between the measured parameters Concentration,
Absorbance and % Transmission.
Switching off Press the <ON/OFF> key.
The meter has an automatic switch-off function in order to save the bat-
function
teries or rechargeable battery (see section 4.4). The automatic switchoff switches off the meter if no key is pressed for an adjustable
period.
The automatic switchoff function is not active
if the power is supplied by the LabStation (optional),
if the Timer or Analysis timer function is on.
During operation with batteries or rechargeable battery the meter automatically switches off the display illumination if no key is pressed for
operation
30 seconds. The illumination is switched on again with the next keystroke.
17
Operation pHotoFlex®STD
1
4.2 Inserting a cell
To be able to insert cells in the pHotoFlex®STD, the cell shaft has to
be prepared to take in a cell.
1 Push the dust cover (1) upward.
The cell shaft for 28 mm cells is open.
Insert a 28 mm cell (see below)
Insert a 16 mm cell (see page 19)
Inserting a 28 mm cell
2 Insert the cell so that it is positioned on the bottom of the cell
shaft.
The cell is ready to be measured.
18
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pHotoFlex®STD Operation
2
3
4
Inserting a 16 mm cell
1 Put the fold-out cell shaft (2) in an upright position until it locks
into place.
2 Pull upwards the height adapter (3).
The cell shaft is extended.
3 Open the external light cover (4) of the cell shaft.
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19
Operation pHotoFlex®STD
4 Insert the 16 mm cell (marking points to the front) so that it is
positioned on the bottom of the cell shaft.
5 Close the external light cover (4).
The cell is ready to be measured.
For optimum measurement results, the cell must always be covered by
the external light cover. Otherwise, the external light can falsify the
measurement result.
4.3 General operating principles
Operating elements,
display
Operating modes,
navigation
This section contains basic information on the operation of the
pHotoFlex
®
STD.
An overview of the operating elements and the display is given in
section 1.2 and section 1.3.
An overview of the operating modes of the pHotoFlex
®
STD and the
navigation through menus and functions can be found in section 4.3.1
and section 4.3.2.
4.3.1 Operating modes
The instrument has the following operating modes:
Measuring
The meter determines measurement data and displays the results in
the measured value display
Calibrating
The meter carries out a blank value measurement or zero adjustment
Data transmission
The meter transmits datasets to the serial interface
20
Configuration
The display indicates a menu with further menus, settings and functions
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pHotoFlex®STD Operation
Photometry
Timer
System
Info
Configuration
Illumination: Auto off
Contrast:
50 %
Brightness
:
50 %
Display
4.3.2 Navigation
Measured value display In the measured value display, you can
select the measured parameter with <M>:
– Concentration
– Absorbance
– % Transmission
open the menu with <MENU>
switch to the superordinate Start menu with <ESC>.
Menus and dialogs The menus for settings and dialogs in procedures contain further sub-
elements. The selection is made with the <><> keys.
The current selection is highlighted as white text on a black background.
Menus
The name of the menu is displayed at the upper edge of the frame.
Menus are opened by confirming with <START/ENTER>. Example:
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Settings
Settings are indicated by a colon. The current setting is displayed on
the right-hand side. With <START/ENTER>, the selection of the
possible settings is opened. Subsequently, the setting can be
changed with <><> and <START/ENTER>.
Example:
Functions
Functions are designated by the name of the function. They are immediately carried out by confirming with <START/ENTER>.
Example:
21
Operation pHotoFlex®STD
i83: A6/25 MC
Celli = 16 mm
iInsert blank sample
Start measurement
Measure blank value
i83: A6/25 MC
Celli = 16 mm
iInsert blank sample
Start measurement
Measure blank value
Messages
Information or operating instructions are designated by the i symbol. They cannot be selected.
The principles of navigation are explained in the two following sections
by reference of examples:
Setting the language (section 4.3.3)
Setting the date and time (see section 4.3.4).
4.3.3 Example 1 on navigation: setting the language
The following example describes in the language of the country how to
set the language. On delivery, English is set as the language in the
®
pHotoFlex
STD. During initial commissioning, the language setting is
done in the menu, Configuration / System / Language.
1 In the measured value display:
Using <MENU>, open the Configuration menu.
The instrument is in the configuration mode.
2 Select the System menu with <><>.
The current selection is highlighted as white text on a black
background.
3 Open the System menu with <START/ENTER>.
22
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pHotoFlex®STD Operation
Language: English
Measured value memory
Display
Reset
Interface
Continue
...
System
Language:
English
Measured value memory
Display
Reset
Interface
Continue
...
System
4 Select the Language menu with <><>.
The current selection is highlighted as white text on a black
background.
5 Open the setting of the Language with <START/ENTER>.
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6 Select the required language with <><>.
7 Confirm the setting with <START/ENTER>.
The setting is active. The menu is displayed in the selected language.
8 To make further settings, switch to the next higher menu level
with <ESC>.
or
Switch to the measured value display with <M> (short pres-
sure).
The instrument is in the measuring mode.
4.3.4 Example 2 on navigation: setting the date and time
The meter has a clock with a date function. The date and time are indicated in the status line of the measured value display. When storing
measured values and calibrating, the current date and time are automatically stored as well.
Numerals are generally entered via the number keys.
23
Operation pHotoFlex®STD
Time:
14:53:40
Date
:
01.02.05
Date format
: dd.mm.yy
Date/time
14:53:40
Time
The correct setting of the date and time and date format is important for
the following functions and displays:
Current date and time
Calibration date
Identification of stored measured values.
Therefore, check the time at regular intervals.
After a fall of the supply voltage (empty batteries or rechargeable battery), the date and time are reset to 01.01.2003, 00:00 hours.
Setting the date, time
and date format
The date format can be switched from the display of day, month, year
(dd.mm.yy) to the display of month, day, year (mm/dd/yy or mm.dd.yy).
1 In the measured value display:
Using <MENU>, open the Configuration menu.
The instrument is in the configuration mode.
2 Select and confirm the System / Continue ... / Date/time menu
with <><> and <START/ENTER>.
3 Select and confirm the Time menu with <><> and
<START/ENTER>.
A display for the entry of numerals with the number keys opens
up.
24
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