Sysmex CA-500 Adjustmant instruction

SECTION 4 ADJUSTMENT

To Cover

4.1 Pressure Adjustment .............................................................................................................................. 1

4.1.1 Adjustment of Pressure Detector Circuit....................................................................................1

4.1.2 Adjustment of Pressure Indication.............................................................................................3

4.2 Detector Block Sensitivity Adjustment .................................................................................................... 4

4.2.1 Required Tools for Adjustment .................................................................................................. 4

4.2.2 Overview of Adjustment Screen (Description)........................................................................... 4

4.2.3 Adjustment of Detector Well for analyzing the scattered light ...................................................6

4.2.4 Adjustment of Detector Well for analyzing the transmitted light

(Chromogenic and Immunoassy)...............................................................................................8

4.2.5 Exit Adjustment .......................................................................................................................... 9

4.2.6 Target Value List for Adjustment ...............................................................................................9

4.3 Mechanical System Adjustment............................................................................................................10

4.3.1 Required Tools for Adjustment ................................................................................................ 10

4.3.2 How to Enter the Mechanical System Position Adjustment Mode ........................................... 10

4.3.3 The Mechanical System Position Adjustment Screen ............................................................. 11

4.3.4 Position Adjustment Procedure of Pipette (“[1]blood 1”, “[2]blood 10”, and “[3]blood_stat”) ...13

4.3.5 Position Adjustment Procedure of Pipette (“[4]reag 1”) (“[5]reag 9”) (“[6]reag 10”) ................. 16

4.3.6 Position Adjustment Procedure of Pipette (“[7]reag_rinse”) (“[8] buffer”) ................................17

4.3.7 Position Adjustment Procedure of Pipette (“[10]wash_o) ........................................................ 19

4.3.8 Position Adjustment Procedure of Pipette (“[11]p_tube 1)....................................................... 21

4.3.9 Position Adjustment Procedure of Catcher (“[17]c_tube 1) .....................................................22

4.3.10 Position Adjustment Procedure of Catcher (“[23]warm 1) .......................................................24

4.3.11 Position Adjustment Procedure of Catcher (“[27]shake) ......................................................... 26

4.3.12 Position Adjustment Procedure of Catcher (“[28]dust) ............................................................27

4.3.13 Position Adjustment Procedure of Catcher (“[29]immunoassy)............................................... 28

4.3.14 Output Setting Values .............................................................................................................. 29

4.4 Temperature Adjustment ...................................................................................................................... 30

4.4.1 Temperature Control Summary...............................................................................................30

4.4.2 Temperature Verification at Each Unit 4 ................................................................................ 30

4.4.3 Temperature Adjustment Procedures 4 ................................................................................. 31

4.5 Electrical Adjustment ............................................................................................................................ 32

4.5.1 PCB No. 6362: MAIN (CPU, I/O, Motor Control, Temperature Control, A/D)..........................32

PCB No. 6373: MAIN-2 (CPU, I/O, Motor Control, Temperature Control, A/D)....................... 32

4.5.2 PCB No. 4086: PS (Power Source, Motor Driver) ................................................................... 34

CA-500 Series S/M Revised December 2001

8

SECTION 4 ADJUSTMENT

4.1 PRESSURE ADJUSTMENT

4.1.1 Adjustment of Pressure Detector Circuit

(1) Verify that the ambient temperature where the unit is located is within the range of 15°C - 35°C. (The

optimum recommended temperature range is 20°C - 30°C.)

(2) Prepare the Pressure Gauge (which can measure the pressure of 0.3 kg/cm

on the rear panel.

2

) in the pressure line (black)

Figure 4-1-1: Rear View

(3) Adjustment procedure:

1) Turn the power OFF. Remove the rear panel and the dummy cover under the HOST connector.

Figure 4-1-2: Dummy Cover (Left Side View)

CA-500 Series S/M 4-1 Revised December 2001

8

2) Connect Jumper Pin J501 of PCB No. 6362 or PCB No. 6373 to C-2.

3) Loosen and remove the rinse bottle cap carefully because the cap will be lifted up by the pressure inside
the rinse bottle.

4) Disconnect the black marked silicone tube connected to the rear panel at the rinse bottle.

5) Connect the pressure gauge to the silicone tube.

6) Turn the power ON.

7) Adjust the VR502 (OFFSET) so that the pressure becomes 225 ± 3 g/cm
increased when VR is turned to CW direction. Disconnect the pressure gauge from the tube once when
adjusting the pressure decreasing.)

8) Remove Jumper Pin J501. (Do not lose the jumper pin because it is placed on the PC board.)

9) Adjust the VR501 (GAIN) so that the pressure becomes 275 ± 3 g/cm
when VR is turned to CW direction. Disconnect the pressure gauge from the tube once when adjusting
the pressure decreasing.)

10) Verify Steps 1) to 5) again. As the VR502 (OFFSET) and the VR501 (GAIN) are related each other, adjust
them repeatedly until the set values are obtained by repeating Steps 1) to 5).

11) Reconnect Jumper Pin J501 to C-1.

12) Disconnect the tube to decrease pressure, and reconnect it.

13) Verify that pressure is 250 ± 3 g/cm
* If pressure is not 250 ± 3 g/cm

2

.

2

, check and adjust again by repeating Steps 1) to 5).

2

. (The pressure will be

2

. (The pressure will be increased

Figure 4-1-3: PCB No. 6362 Jumper, VR Location

CA-500 Series S/M 4-2 Revised December 2001

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4.1.2 Adjustment of Pressure Indication

(1) Verify that the ambient temperature where the unit is located is within the range of 15°C ~ 35°C. (The

optimum recommended temperature range is 20°C ~ 30°C.)

(2) Connect the Pressure Gauge (with resolution of more than 28 g/cm

300 g/cm

2

) to the pressure line (black) on the rear panel.

(3) Adjustment procedure:

1) Start Service Mode.

2) Select [Special Operate] Æ [Service] Æ [Adjust] Æ [Adjust. of Pump].

2

, which can measure the pressure of

Figure 4-1-4: Adjust Pump Screen

3) Disconnect the tube at the pressure side (the black marking line on the rear panel) to release the

pressure sensor (so that the pressure sensor shows the atmospheric pressure).

4) Offset the cursor by using [↑] and [↓] keys.

5) Adjust the offset value by using [+] and [-] keys so that the pressure gauge shows 1 g/cm

the offset value is lowered, A/D value also becomes lower.) (Actually, there is a fluctuation of 0 ~ 4
for the value, therefore, adjust it so that it shows the value, which approaches 0 most.)

6) Re-connect the pressure gauge to the pressure line on the rear panel.

7) Move the cursor to the gain by using “↑” and “↓” keys.

8) Adjust the gain value by using [+] and [-] keys so that the pressure gauge value (adjusted 250 g/cm

by the procedure described in 4.1.1 Adjustment of Pressure Detector Circuit) agrees with the
displayed pressure value on CA-500. The difference between the pressure gauge value and
CA-500 displayed value should be adjusted within 3 g/cm

2

.

9) Verify Steps 3) to 8) again, and if the value is within the specified range. Press the [Return] key.

10) Confirmation screen will be displayed by pressing “Return” key.

REFERENCE: Pressure is adjusted at the factory in principle, therefore, it is not necessary to adjust

in the field. Setting values are stored in EEPROM on the PCB No. 6362 or PCB No.

6373.
In case of erasing the EEPROM, these printed values are attached on the memory
card socket.

Select the appropriate key.
FIX: The new setting value is fixed.
Cancel: The new setting value is deleted and Adjust of Pump program will be quitted.
Continue: The adjustment can be continued.

2

. (When

2

CA-500 Series S/M 4-3 Revised December 2001

8

4.2 DETECTOR BLOCK SENSITIVITY ADJUSTMENT

4.2.1 Required Tools for Adjustment

(1) CA-Series Standard Scattering Stick Set
(2) Reaction Tube
(3) Micro Pipette
(4) Ebonite Stick for adjusting offset value

4.2.2 Overview of Adjustment Screen (Description)

(Adjustment procedure starts from 4.2.3 Adjustment of Detector Well for Analyzing the Scattered Light)

(1) Verify that the ambient temperature where the unit is located is within the range of 15°C - 35°C.

(The optimum recommended temperature range is 20°C - 30°C.)

(2) Start Service Mode.
(3) Select [Special Operate] Æ [Service] Æ [Adjust] Æ [Detector Gain].
(4) The Detector Gain screen will be displayed.

Figure 4-2-1: Detector Gain Screen

1) To change or adjust a value (Target, Offset, Gain), select the value by using [↑] and [↓] keys.

The selected value is displayed in the reverse video, and inputting by pressing [+] and [–] keys or
inputting numerals in the [Manual Entry] screen is available.

2) Select a channel among CH1 (Detector Well 1) Æ CH2 (Detector Well 2) Æ CH3 (Detector Well 3) Æ

CH4 (Detector Well 4) Æ CH5 (Detector Well 5) Æ CH6 (Detector Well 6) in the order by pressing
[Channel Change] key.

3) Press [Sensitive] key to alternate the sensitivity of the selected channel in the order of

High Gain Æ Low Gain Æ PT Gain.

4) Start the automatic adjustment for the offset/gain value by pressing [Auto Adjust] key.

CA-500 Series S/M 4-4 Revised December 2001

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(5) Press [Manual Entry] key to display the screen that the manual entry by numeric keys is available.

Figure 4-2-2: Manual Entry Screen

1) Pressing the numeric keys [0] to [9] can set the desirable value directly.

2) Pressing [C] key can clear the set value.

3) Press [Enter] key to settle the set value. The set value cannot be settled if [Quit] key is pressed

without pressing [Enter] key.

4) Press [Quit] to return the previous screen.

CAUTION: 1) This adjustment should be done more than 30 minutes after the power-ON the

instrument.

2) Use the scattering stick after being heated for more than 10 minutes in the detector
well or the incubation well.

3) Close the light shield cover to avoid the influence by the external light during
adjustment.

4) When Detector Adjustment Error occurs, the channel No. with the error is added to
the error message. 13

Note: 13 1) Transmitted Light calibration will be performed automatically before the first

measurement after switching on the instrument or after 24 hour continuous operation. It
will be performed when a chromogenic or immunologic parameter is selectable in the
actual group setting.

2) Instruments with new main board (PCB NO.6373) are able to switch off LEDs during

standby operation automatically in order to prolong the life time of the LEDs.

CA-500 Series S/M 4-5 Revised May 2003
13 ECR 303C012

4.2.3 Adjustment of Detector Well for analyzing the scattered light

(1) Offset adjustment

1) Insert the ebonite stick for adjusting the offset value in the detector well to be adjusted.

Detection Well
(Immunoassy)

(CH6)

Figure 4-2-3: Detector Well Positions

2) Close the light shield cover.

3) Select channel 1 by using [Channel Change] key, select Low Gain by using [Sensitive] key, and select

[Offset] position by pressing [↑] or [↓] key.

4) Press [Auto Adjust] key to start the automatic offset adjustment, and when the adjustment is
completed, the alarm sounds.

5) Change Gain to PT Gain and enter Offset value manually in executing Step 3). (Press [+] and [–] keys
or [Manual Entry] key to adjust manually.)

6) Execute Steps 1) to 5) for channels 2 to 4.

(2) High Sensitivity Adjustment (Gain Adjustment)

1) Insert the heated high sensitivity scattering stick (labeled usually as “750”) into the detector well to be
adjusted (CH1 to 4).

2) Close the light shield cover.

3) Select channel 1 by using [Channel Change] key, select High Gain by using [Sensitive] key, and select
[Gain] position by pressing [↑] or [↓] key.

4) Press [Auto Adjust] key to start the automatic gain adjustment, and when the adjustment is
completed, the alarm sounds.

5) Execute Steps 1) to 5) for channels 2 to 4.

CA-500 Series S/M 4-6 Revised December 2001

8

(3) Low Sensitivity Adjustment (Gain Adjustment)

1) Insert the heated low sensitivity scattering stick (labeled usually as “430”) into the detector well to be
adjusted (CH1 to 4).

2) Close the light shield cover.

3) Select channel 1 by using [Channel Change] key, select Low Gain by using [Sensitive] key, and select
[Gain] position by pressing [↑] or [↓] key.

4) Press [Auto Adjust] key to start the automatic gain adjustment, and when the adjustment is
completed, the alarm sounds.

5) Change the sensitivity to PT Gain, and verity that both Offset and Gain values for PT Sensitivity are
automatically set when changing Offset and Gain values for Low Sensitivity.

To set PT sensitivity individually, execute the same procedure as (1) Offset Adjustment and (3) Low

Sensitivity Adjustment or enter values manually using numeric keys.

6) Execute Steps 1) to 5) for channels 2 to 4.

NOTE: The gain adjustment and the offset adjustment have an influence on each other,

therefore, reconfirm that the set value after each adjustment is within the specified
range of values. Refer to 5.9.2 Detector Block Adjustment Value for default value.

CA-500 Series S/M 4-7 Revised December 2001 8

4.2.4 Adjustment of Detector Well for analyzing the transmitted light

(Chromogenic and Immunoassy)

(1) Offset adjustment

1) Insert the ebonite stick for adjusting the offset value in the channel 5 of the detector well.

Detection Well
(Imm unoassy)

(CH6)

Figure 4-2-4: Detector Well Positions (Chrom, Immunoassy)

2) Close the light shield cover.

3) Select [CH5] by using [Channel Change] key and select [Offset] position by pressing [↑] or [↓] key.
(Low Gain is fixed and cannot be changed.)

4) Press [Auto Adjust] key to start the automatic offset adjustment, and when the adjustment is
completed, the alarm sounds.

5) Execute Steps 1) to 4) for channel 6.

(2) Gain adjustment (Dynamic Range adjustment)

1) Dispense 200 µL of distilled water or buffer (OV-30) into a reaction tube in advance, set it into the
transmitted light detector well (channel 5), and heat it up for approximately 10 minutes.

2) Close the light shield cover.

3) Select [CH5] by using [Channel Change] key and select [Gain] position by pressing [↑] or [↓] key.
(Low Gain is fixed and cannot be changed.)

4) Press [Auto Adjust] key to start the gain adjustment, and when the adjustment is completed, the alarm
sounds.

5) Open the light shield cover, rotate the reaction tube set in the channel 5 detector well and verify that
the indicated A/D value does not exceed 4,000. At this time, avoid the external light as much as
possible. If the A/D value exceeds 4,000, set the reaction tube at the position where the indicated A/D
value is the biggest and press [Auto Adjust] key again to start the adjustment.

6) Execute Steps 1) to 5) for channel 6.

CA-500 Series S/M 4-8 Revised December 2001

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4.2.5 Exit Adjustment

(1) When the adjustment is completed, press [Return] key.
(2) FIX, Cancel the set value, and continue the adjustment:

1) When the adjusted value is settled and the adjustment is completed, press [FIX].
Store the new setting value in the memory and output the value on the built-in printer, and then return
to the sub-menu.

Figure 4-2-5: Output Example

2) When the adjusted value is discarded and the adjustment is completed, press [Cancel] key. In this
case, the setting value data is not changed and returned to the sub-menu.

3) When the adjustment is continued, press [Continue] key.
In this case, the screen returns to the previous set screen, so you can continue the adjustment.

4.2.6 Target Value List for Adjustment

Detector Well Specified Values 19
(A/D Display Value for Offset and Gain)

Detector Well CH1 ~ 4 CH5 CH6

Tools HIGH LOW PT LOW LOW

OFFSET Ebonite Stick < 30 1 - 20 1 - 20

480* Scattering Stick – 480 ± 15 480 ± 15 – –

GAIN 910* Scattering Stick 910 ± 20 – – – –

Distilled Water – – – 3800 - 4000 3800 - 4000

*Varies lot by lot.

CA-500 Series S/M 4-9-1 Revised February 2004
19 TB200408

4.2.7 LED Auto Calibration 13

(1) Press [Special Operate]-[Calibration] keys. The LED Calibration screen will appear.

Sysmex

Ready
Replace Rack? YES!

LED Calibration

Target Value

Vial Type

Please set Calibrator

on Reagent Holder 1

900

Cup

and set Clean1 on
Reagent Holder 11.
Press [set] key.

Set

(2) Enter a target value.
Enter an indicated value (100 - 999) which is given in the calibrators table of assigned values for

calibration using the numeric keys, and press [Enter] key.
(3) Select a container of the calibrator for calibration.
Move the cursor to “Vial Type” using [↑] and [↓] keys, and press [Next] key to select the container.
(4) Set the calibrator for calibration to the reagent holder 1 and set CA CLEAN I to the reagent holder 11.
(5) Press [Set]-[OK] keys to start LED calibration.
When the operation is completed, the LED Calibration Update Confirmation screen will appear.

Sysmex

Ready
Replace Rack? YES!

LED Calibration Update Confirmation

IP

7

4

1

0

C

89

5

2

6

3

Enter

Quit

IP

(1)

45

*OK

CH2

130

OK

CH1

Too weak to use:
Weak, but still available:

CH3

ERR1

22

CH3
CH1

CH4

130

OK

(2)

(3)

Please refer to Operator's Manual

Set

Cancel

Displayed contents

(1) Channel No.

(2) LED status

(3) OK: Available

*OK: Available. However, replacement is required within a few months.

ERRxx: Not available
(6) When updating a new adjustment value, press [FIX] key.
When the status of all channels is OK
The new adjustment value is saved, and returns to the LED Calibration screen.
When there is a channel with a calibration error
The confirmation screen will appear.
Press [Cancel] key to return to the LED Calibration Update Confirmation screen.
Press [OK] key to save the new adjustment value, and return to Calibration screen.
(7) Take out the calibrator for the calibration, and set the former reagent.

CA-500 Series S/M 4-9-2 Revised May 2003
13 ECR 303C012

4.3 Mechanical System Adjustment

4.3.1 Required Tools for Adjustment

(1) Sample Cup (small)
(2) Reaction Tube
(3) Collection Tube (15 mm diameter)
(4) Dade 1 mL Reagent Bottle
(5) Push Vial Bottle
(6) Water (distilled water)
(7) Dispensing Pipette (50 and 60 µL)
(8) CA-5H

4.3.2 How to Enter the Mechanical System Position Adjustment Mode

(1) Start the service mode by pressing “ID No. Entry”, “C”, “9”, “-”, “0”, “Enter”, and “Quit”.

(2) The screen changes from “Main Menu” to “Mecha Position” by pressing keys of [Special Operate] Æ

[Service] Æ [Adjust] Æ [Mecha Position].

Select the unit to be adjusted on this screen and perform the position adjustment.

Z-axis Position Adjustment Tool PM (031-0171-4) 13

CA-500 Series S/M 4-10 Revised May 2003
13 ECR 303C012