Roche Light Cycler Service manual

Service Manual

Light Cycler

Final 1.0 - January 1999

Light Cycler

Service Manual

Chapter	1	Application/Introduction
Chapter	2	Installation
Chapter	3	omitted
Chapter	4	Mechanics
Chapter	5	Electronics
		Note: In some wiring diagrams the
		fluorimeter is termed photometer !
Chapter	6	Software
Chapter	7	Trouble Shooting
Chapter	8	Spare Parts
Chapter	9	omitted
Chapter	10	Maintenance

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1Application/Introduction _________________________________ 2

1.1LightCycler Workstation ______________________________________ 2

1.2Technical Data ______________________________________________ 5

1.2.1Instrument Specifications __________________________________________ 5

1.2.2Application Specifications __________________________________________ 6

1.2.3Detector Specifications ____________________________________________ 7

1.3System Description __________________________________________ 8

1.3.1The LightCycler ___________________________________________________ 8

1.4Introduction _______________________________________________ 11

1.4.1Instrument ______________________________________________________ 11

1.4.1.1Principle of the LightCycler Technology ______________________________ 11

1.4.2Instrument Components of the LightCycler ___________________________ 12

1.4.2.1The Cycler Component _____________________________________________ 12

1.4.2.2The Fluorimeter Component ________________________________________ 13

1.5Warranty __________________________________________________ 14

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1 Application/Introduction

1.1LightCycler Workstation

Contents of the Workstation

The LightCycler components are listed in the following table:

Component	Description
System component 1	· LightCycler™ Instrument
	· Sample Carousel (for Ø 1.5 mm capillary), premounted in LightCycler™
	Instrument
System component 2	· Capillaries, (96 capillaries and caps/box)
	· 32 Centrifuge adapters with 1 cooling block in an aluminium cooling block
	· LightCycler™ Operator´s Manual
	· Software Package
	· Cable (to connect LC to computer)
	· Power cord (German plug)
	· Power cord (U.S. plug)
	· Mouse Pad
Recommended PC Hardware /8/98	· Vectra VE4 5/200 MMX Model 3200 Desktop
	· Intel Pentium MMX processor, 200 MHz
	· 64 MB SDRAM DIMM
	· 3.2 GB ultra-ATA/33 hard disk
	· On-board PCI vide: S3 Trio 64 V2, 2MB 50 ns DRAM
	· 24x speed DIE CD-ROM disk drive
	· Keyboard and PS/2 mouse
Operating system	Windows NT 4.0, including service pack III
ZIP drive	Internal Iomega ZIP drive, DIE interface (ATAPI)
Monitor	HP Ergo 1280, 17" monitor (HP# D2840A)
Printer	HP Deskjet 890C Color Inkjet Printer

Tab.: cap1-1

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Marks of Conformity

The LightCyclerTM has been manufactured in accordance with EN 61010-1 (Safety Regulations for Measuring, Control and Laboratory Instruments; Part 1: General Requirements (IEC 1010-1 + A1: 1992, modified) and has been checked according to all relevant safety standards prior to leaving the factory.

The instrument has been approved for use by recognized testing institutions.

This is confirmed by the following test/conformity symbols:

GS	Certificated by VDE institute (Association of German
	Electrotechnical Engineers)

CE								The instrument conforms to current directives as issued by the
								European Union

UL	Certificated by Underwriters Laboratories Inc.

CUL	Certificated by Underwriters Laboratories for Canada – a
	testing facility recognized by the Standards Council of Canada
	(SSC)

Tab.: cap1-2

All equipment to be connected must fulfill the standards set by IEC 950 (Safety of Information of Technology Equipment, Including Electrical Business Equipment).

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Classification

-ISM instrument, medium-sized, for industrial, laboratory and domestic use.

-The instrument is designed for stationary operation.

-Any false measurements produced are irrelevant from the safety point of view.

-The instrument is designated for worldwide marketing.

-It is intended for evaluating pre-processed biological material.

The following should be noted:

-The instrument may not be used in conjunction with infectious material without additional safety measures being taken.

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1.2	Technical Data
1.2.1	Instrument Specifications
General Data
Dimensions		Height 45 cm
		Width 30 cm
		Depth 45 cm
Weight		20 kg
Power supply		110-240 V ± 10%, 47-63 Hz
Wattage		800 W
Noise acc. to DIN 43635		< 80 dBA
Heat emission, including PC, monitor and printer		Max. 860 kcal/h
Safety symbols		CE, GS, UL, CSA or CUL
		Tab.: cap1-3
Environmental Parameters
Temperature during operation		15 – 35°C
Temperature required during operation to maintain		15 – 30°C
specifications
Temperature during transport/storage/packaging		20 – 60°C
Relative humidity		20 – 80%
Altitude / pressure		0 – 2000 m above sea level1030 – 850 hp
		Tab.: cap1-4
Samples
No. of samples		32
Sample volume		2 – 20 µl
		Tab.: cap1-5

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1.2.2 Application Specifications
PCR Temperatures
	Temperature range	40 – 98°C
	Temperature accuracy of measuring system	± 0.3°C
	Heating rate
	Heating rate 40 – 95°C, step 40 – 95°C, time 10 – 90%	8s
	Heating rate 50 – 72°C, step 50 – 72°C, time 10 – 90%	4s
	Heating rate 72 – 98°C, step 72 – 95°C, time 10 – 90%	5s
	Cooling rate
	Cooling rate 95 – 40°C, step 95 – 40°C, time 10 – 90%	8s
	Cooling rate 95 – 60°C, step 95 – 60°C, time 10 – 90%	4s
	Temperature stability
	Temperature stability with respect to time, 95°C	+1°C / -0.5°C range
	Temperature stability with respect to time, 65°C	+1°C / -1.0°C range
	Temperature stability with respect to time, 40°C	+1°C / -0.3°C range
	Temperature homogeneity
	Temperature homogeneity over all capillaries and time, 95°C	± 1.5°C range
	Temperature homogeneity over all capillaries and time, 65°C	+ 0.7°C / -1.5°C range
	Temperature homogeneity over all capillaries and time, 45°C	+ 1.5°C / -0.5°C range
Temperature Gradients		Tab.: cap1-6
	Temperature gradient 0.1 °C/s, 0.2°C/s
	Temperature homogeneity for one capillary, 70 – 95°C	± 1.5°C range
	Temperature homogeneity for one capillary, 50 – 70°C	± 1.0°C range
	Temperature homogeneity for one capillary, all positions, 70 – 95°C	± 2.0°C range
	Temperature homogeneity for one capillary, all positions, 50 – 70°C	± 1.5°C range
	Temperature gradient 1.0 °C/s
	Temperature homogeneity for one capillary, 70 – 95°C	± 2.0°C range
	Temperature homogeneity for one capillary, 50 – 70°C	± 2.0°C range
	Temperature homogeneity for one capillary, all positions, 70 – 95°C	± 3.0°C range
	Temperature homogeneity for one capillary, all positions, 50 – 70°C	± 3.0°C range
	Temperature homogeneity for control of temperature peaks
	Stability at 95°C for dynamic cycles, 72 – 95 – 50°C, 0s maintained	+2.0°C / -1.0°C range
	Homogeneity of capillary /capillary at 95°C for dynamic cycles, 72 – 95 –	+3.0°C / -1.0°C range
	50°C, 0s maintained
	Temperature homogeneity in capillary
	20 µl, 70°C	± 1.0°C range
	15 µl, 70°C	± 0.5°C range
		Tab.: cap1-7

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1.2.3 Detector Specifications
Illumination
Type		LED
Median wavelength		470 nm
Output at 470 – 490 nm		0.1 mW
		Tab.: cap1-8
Detector
Type		Photohybrid
Receptor surface		2.5* 2.5 mm²
Sensitivity at 520 nm, 20 µl sample volume		15 nM fluorescein (1s)
Sensitivity at 650 nm, 20 µl sample volume		x nM fluorescein /LightCycler Red 640
Sensitivity at 700 nm, 20 µl sample volume		x nM fluorescein / n.d.
Electronic dynamic		12 bit
Electronic bandwidth		1 kHz
Integration time		20 ms
Alteration of amplification range		Factor 1 – 256
		Tab.: cap1-9
Filter
Detector 1	Bandpass 520 nm HBW 20 nm, dichroitic
Detector 2	Bandpass 645 nm HBW 30 nm, dichroitic
Detector 3	Bandpass 710 nm HBW 40 nm, dichroitic
		Tab.: cap1-10
Time
Measuring time for 32 capillaries	< 5s
		Tab.: cap1-11

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1.3System Description

1.3.1The LightCycler

Scheme of the Instrument

Fig. scheme1

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Description of the Instrument

The sample capillaries are thermostatted with hot air in the thermal chamber; a ventilator ensures efficient aspiration, distribution and temperature homogeneity of the air during the heating process. During the cooling process, the ventilator operates at a higher speed in order to ensure adequate cooling. A heating coil controls the temperature.

For measurement, the sample rotor is propelled by a stepper motor; this brings the capillary tip precisely to the focal point of the photometer optics. The photometer itself is placed in a radial position in relation to the signal maximum in order to compensate for any radial deviation of the capillary tip.

3 microprocessors incorporated in the instrument control the process. Processor 1 is for communication, processor 2 regulates the temperature and processor 3 controls the measurement procedure as well as the rotor and photometer movements.

For online display, data are transmitted to the PC. Here, sample data are entered, the PCR reaction temperature is monitored, measurement modes and initial evaluation of melting point temperatures are carried out.

Structure of the LightCycler

The instrument comprises a basic lower and an upper unit.

Basic Lower Unit	Thermo-chamber, photometer, drive units, circuit boards and power supply are located in
	the basic unit. The base of the unit is a 10 mm cast aluminum plate onto which the various
	elements are fixed. This guarantees stability, especially for the thermo-chamber and
	photometer.
Upper Unit	The upper unit contains the heating coil, an excess temperature sensor and a cooler with
	sectional converter.
	Tab.: cap1-12

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Thermo-Chamber Thermostatting

Thermostatting is done by using hot and cold air. A sensor with thermal time constants identical with those of the capillaries provides reference values for control purposes. The process is controlled by varying the heating coil voltage. During the heating phase, only the ventilator in the thermo-chamber is in operation; this guarantees a high degree of temperature homogeneity. During the cooling phase, both the capillaries and the heating coil must be cooled. The ventilator is then operated at a higher speed.

2 sensors are incorporated in order to prevent excess temperatures:

Sensor I	Sensor I is responsible for the temperature regulation.
Sensor II	Sensor II monitors the actual temperature.

Tab.: cap1-13

Fluorescence Photometer

A 3-channel fluorescence photometer is used for detection purposes. Sample excitation is initiated by a blue LED with an emission maximum of 470 nm.

Fluorescence is detected by means of a separate photohybrid for each wavelength.

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1.4Introduction

1.4.1Instrument

1.4.1.1 Principle of the LightCycler Technology

System Description

The LightCycler comprises two different instrument components: a cycler component and a fluorescence detection component. The combination of both components allows complex applications such as product analysis, quantification and mutation analysis.

The cycler component has been optimized for rapid PCR applications. Compared with classical PCR where typical cycling programs take several hours, PCR analyses carried out in the LightCycler can be completed in only 15-30 min. This considerable time saving effect is due to very short temperature transfer times, the socalled „ramping“ times, of the LightCycler instrument. „Ramping“ times are the time periods necessary to reach the next pre-set temperature of a „cycling“ protocol in a PCR experiment. An example is the transition from 94°C to 55°C after sample denaturation for primer hybridization during the annealing phase. This sort of high-speed cycling is made possible by an optimized surface / volume ratio of the samples and the use of air as a temperature transfer medium.

Fluorimetric Detection

The combination of an optimized thermocycler and a fluorimeter not only enables “online” presentation of the PCR data obtained and efficient analysis of results, but also offers the user utmost flexibility. To meet individual requirements, different detection formats can be used by choosing and combining fluorescent dyes and detection probes.

Fluorimetric detection of the PCR products formed consists of two different procedures:

-sequence-specific detection by the double stranded DNA binding dye SYBRÒ Green I, or

-sequence-specific oligonucleotides are coupled to suitable fluorophores as hybridization probes.

Analysis of Results

By means of the special LightCycler software, the data obtained from fluorimetric analysis can be evaluated and displayed. Additional cumbersome analytical procedures subsequent to PCR, e.g. restriction and gel analysis as well as blotting and hybridization experiments are no longer necessary. The direct and simple analysis of results also minimizes the contamination risk posed by samples and working materials due to a reduction of working steps required.

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1.4.2Instrument Components of the LightCycler

1.4.2.1 The Cycler Component

Sample Carousel

The central element of the cylindrical thermal chamber is the sample rotor. The rotor takes 32 samples in glass capillaries with a diameter of 1.55 mm and a length of 35 mm. PCR analysis carried out in these specially manufactured glass capillaries reduces the reaction volume to only 5 to 20 ml max. The optimized surface- to-volume ratio thus obtained allows for very short cycle times of only 15 to 20 seconds per cycle. The very favorable surface-to-volume ratio also guarantees an extremely rapid temperature change in the reaction mixture during each PCR cycle. At the same time, the glass capillaries serve as cuvettes for the fluorimetric determination of the PCR products formed.

Fig. scheme1

Thermal Chamber

The cylindrical thermal chamber is supplied with hot air and air at ambient temperature by means of a fan with coupled heating coils. The incoming air is homogeneously distributed by a high-velocity fan at the base of the instrument, thus creating an even temperature throughout the reaction chamber. Surplus air is vented through an opening at the side of the chamber. The temperature is controlled by using an integrated measuring system with a temperature sensor installed in the direct vicinity of the sample capillaries of the sample carousel. The PCR thermal chamber is directly coupled to the optical system of the fluorimeter.

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1.4.2.2 The Fluorimeter Component

Optics

The fluorescent optical components of the LightCycler are schematically illustrated in chapter 1.3.1 The Light Cycler. A blue high-performance diode (blue LED) serves as energy source for sample excitation. The diode emits radiation which is spectrally filtered to a wavelength of 470 nm and an energy of 1 mW and is collimated (brought to the same wavelength) by means of a special optical system. The homogeneous light beam of 470 nm generated is subsequently focused onto the individual glass capillaries and then onto the samples to be measured in the sample carousel. The glass capillaries are moved by a high-precision stepper motor and placed in the optimal position for excitation and measurement. According to the spectral properties of the fluorophore used, the light emitted from the excited samples is guided into one of three channels (see drawing in chapter 1.3.1 The Light Cycler) via dichroitic mirrors for subsequent evaluation. In this channel, the emitted light beam is further collimated and spectrally concentrated by means of a special filter optical system. For final evaluation, the light beam is then focused onto a photomultiplier.

The three evaluation channels of the LightCycler are fitted with filter combinations. Thus, analysis at the given emission wavelengths is possible and exact sample measurement can be carried out in parallel with the fluorophores given in Tab.: cap 1-14:

Fluorophore	Excitation Channel		Analysis Channel
		1			3
				2
	470 nm±40	530 nm±20		640 nm±20	710 nm±40
Fluorescein	490 nm	525 nm
SYBR Green I	494 nm	521 nm
LC-Red 640*				640 nm

Tab.: cap1-14

Table cap 1-14

Specification of excitation and measuring channels of the fluorimeter component of the Roche Diagnostics LightCycler: Comparison of the spectral properties of selected fluorophores.

Abbreviations *: LC-Red = Light CyclerTM -Red 640

**: LC-Red is not excited by the blue LED, but is a FRET partner of fluorescein.

For further information, refer to the Operating Manual.

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1.5Warranty

Processing of Warranty Claims

A warranty claim has to be processed by way of the Return Authorization procedure or any accepted equivalent. Please answer all the questions on the RA form with the greatest care.

The warranty claim will only be accepted by the manufacturer if adetailed fault description is supplied. Complete instruments are accepted only in agreement with the Technical Product Management or with the responsible person of Roche Diagnostics Technical Support.

Important Information:

-Only parts marked with „A“ in the price list are generally accepted under warranty.

-Return only parts marked with „R“ in the spare parts price list.

-Warranty claim for “R” parts will be accepted, if the part has been returned to Mannheim.

-All defective parts ( non-“R“ and „A“ parts ) should be kept for a period of 7 months, should the manufacturer need to investigate the part, in which case it will be requested.

-All parts unduly returned to Mannheim will be sent back at the expense of each country's respective service center.

-RA warranty claims are accepted no later than 8 weeks after the problem date.

Exclusion from Warranty

The aforementioned warranties do not apply in case of improper use, handling, transportation or storage, faulty installation, repair or maintenance, chemical influence or contamination as well as damages resulting from that, failure to follow operating instructions, alterations or modifications of instruments or parts thereof not authorized or recommended by Roche Diagnostics and resulting damages, normal wear and tear and in case of other circumstances beyond the control of Roche Diagnostics.

Repairs

As a general rule, all instrument repairs should be carried out by authorized and trained personnel only.

Repair of Parts Marked with „R“

Parts which are worth repairing are marked with „R“ in the spare parts price list. New and repaired parts can be distinguished from each other by different material numbers (language version).

(e.g. new part: 1234567-001, repaired part: 1234567-

984)

Repaired parts should be ordered together with new parts via the order processing department in Mannheim (OU-VDG). Defective parts should be returned together with order of repair, including the filled out RA form (giving full details of the defect and marked choice box with repair), to Logistic Instruments (Incoming Goods) in Mannheim-Wohlgelegen (LI-LV).

Repair of Instruments

Complete instruments are accepted only, if the problem is definitety located in the photometer unit. The replacement should have been agreed with the Technical Product Management or with the responsible person of the Roche Diagnostics Technical Support.

Terms of Delivery

Shipments to countries with the routine truck are c.i.f./ other shipments are ex works Mannheim .

Emergency shipments incur additional charges.

Charges

"Repaired" parts (material no. 1234567-984) are supplied at a "repair price. There will be a subsequent charge consisting of the price difference between a new and repaired part, should the defective parts not be returned within 3 weeks (for European countries) and 8 weeks (for Overseas countries).

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After receipt of a warranty claim for “A” parts, BMG will credit 100 % of the currently effective ex MA price.

In case the manufacturer does not accept the warranty claim, each country's respective service center will be charged the R-price for "R" parts and the new price for non "R" parts.

RA Form

Return Authorization

Please answer all the questions on the RA form with the greatest care and sign the form.

-Country code

-Problem date

-Type of instrument

-Serial no. of the instrument

-Installation date of instrument

-Defective instrument or spare part

-Part number and material number of the spare part

-Old / new serial no.

-Fault description

-Alarm code

-Service / workshop report no.

Instruments Not Under Warranty

- Installation date of spare part

All returned parts should be individually labeled with the corresponding RA no. and shipped together with the completed RA form to:

Roche Diagnostics

Logistic Instruments

RA Management

Friedrich Ebert Str. 100

D - 68167 Mannheim

Germany

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RA Form

(Example)

ROCHE DIAGNOSTICS GmbH
Friedrich-Ebert-Strasse 100	Telefon : [phone]
D-68167 Mannheim	Fax :	[phone]
Germany

Return Authorization

No.:

Country code:

Date:

Instrument:

Serial No.:

Installation date:

Spare Part:

Customer:

Address:

(will be filled in by BM)

Part No.:

Qty.:

Part Name:

Repair

Comments

Mat.-No.:

Warranty

Installation date of Spare Part:

Warranty Repair

OLD serial No.:

Modification

NEW serial No.:

Replacement

Fault Description:

Alarm Code:

Service Report No.:

Workshop Report No.:

Place:

Date:

Signature:

Remarks (will be filled in by BM)

NOS

Credit

FC

BM

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2.Installation _____________________________________________ 2

2.1Installation Requirements ____________________________________ 2

2.2Installation of the LightCycler _________________________________ 4

2.3Computer and Software Installation ____________________________ 5

2.4LightCycler De-installation ____________________________________ 6

2.5Operation and Maintenance of the LightCycler ___________________ 7

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2.Installation

2.1Installation Requirements

Note

-The LightCycler should not be set up next to instruments that cause electromagnetic interference or have high inductance, e.g. centrifuges or mixers.

-All connected peripheral instruments must fulfill the requirements of Standard IEC 950 (UL 1950).

-All plugs used in the LightCycler workstation (PC, printer, monitor) should have the same phasing in order to prevent switch-on peaks and electronic noise generated by other instruments or the power supply itself. Use of an appropriate distributor plug with the LightCycler workstation is recommended.

Working Place Requirements

See the following table for the working place requirements:

Dimensions and weight	The LightCycler has a width of 30 cm, a depth of 45 cm and a height of 45 cm.
	It weighs approximately 20 kg.
Voltage requirements	The LightCycler operates on 120 – 240 V (50 – 60 Hz) and need not be
	adjusted.
Power consumption	The LightCycler requires approximately 800 W; PC and printer require a further
	500 W.
	Tab.: cap2-1

The LightCycler must not be opened by the user

The fuse may only be replaced if the instrument has been switched off at the mains.

Environmental Requirements

Please refer to the following table for the environmental requirements:

Ambient temperature	15	– 35 °C (all specifications maintained between 15 and 30°C)
Humidity	20	– 80 %, no condensation
Altitude	Sea level to 2000 m
Excess voltage	category II
Degree of contamination	2

Tab.: cap2-2

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Storage Conditions

The LightCycler™ can be stored on5 the following conditions:

Ambient temperature	-20 - +60°C
Humidity	20 – 80%, no condensation

Tab.: cap2-3

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2.2Installation of the LightCycler

Installation of the Instrument

Please refer to the following table for the installation of the LightCycler instrument:

Step	Action
1	Unpack the instrument.
2	Position instrument on bench, working surface. Allow 10 cm space left, right and behind.
3	Electrical connections:
	-	Connect LightCycler to the PC using an R 232 cable (serial inter-face).
	-	Connect LightCycler, PC, monitor and printer to same distributor plug.

Tab.: cap2-4

Ensure that printer, monitor and PC have been set to the correct voltage.

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2.3Computer and Software Installation

Installation of the PC

After unpacking the computer, carry out the following steps:

Step	Action
1	Connect mouse, keyboard and monitor to the computer.
2	Connect the LightCycler to the computer with an R 232 cable (serial interface).
3	Connect computer, monitor and LightCycler to the distributor plug.
	The computer is now ready for operation
	Tab.: cap2-5

Software

The complete software package is installed by Roche

Diagnostics.

Installation of Updates / Reinstallation

In the following table you find information about the reinstallation and loading of updates:

Step	Action
1	Store any data, e.g. DATA, PROFILES and PROTOCOLs
2	Place LightCycler CD-ROM in appropriate disk drive (e.g. F)
3	Start set-up program (e.g. F:\SETUP.EXE).

Tab.: cap2-6

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2.4LightCycler De-installation

De-installation of the Instrument

Please refer to the following table for the de-installation of the instrument:

Step	Action
1	Switch off the instrument.
2	- Disconnect the RS 232 and power cables.
	- Clean according to the chapter on service and decontaminate if necessary.
3	Dispatch instrument in its original packaging.

Tab.: cap2-7

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2.5Operation and Maintenance of the LightCycler

Guidelines for Operation

-The instrument may be operated only if protected from the weather. It may not be operated in buildings without temperature regulation facilities. If necessary, additional drying agents may be used to eliminate humidity.

-The instrument should not be operated near dripping, spraying, splashing or running water.

-The instrument is thus suitable for use according to classification 3K3 in accordance with Standard EN 60721-3-3.

-The instrument may be used at locations subject to noticeable or significant vibration; however, it should not be exposed to higher levels of shock waves.

-The instrument is thus suitable for use according to classification 3M4 in accordance with Standard EN 60721-3-3. The instrument is able to tolerate vibrations up to classification 3M6. However, based on expected locations of use, no significant and permanent vibrations or shock waves are to be expected.

Transport and Storage

When storing or transporting the instrument, it should not be exposed to extreme cold, e.g. as in the case of air freight. Temperatures lower than –25°C should be avoided (damage to optical systems). The optical systems are equipped with open ventilation systems and should therefore be protected from dirt and humidity. There are no further specifications for transport and storage.

General Maintenance

The instrument is maintenance-free.

Cleaning the Instrument

-Use 70% ethanol for disinfecting the incubator.

-Use only alcohol and an optical cloth for cleaning the optical window.

-Use a mild commercial detergent for domestic use for cleaning the housing.

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4Mechanics _____________________________________________ 3

4.1Overview __________________________________________________ 3

4.2Disassembly of the Housing Parts _____________________________ 4

4.2.1Housing Lid ______________________________________________________ 4

4.2.2Housing _________________________________________________________ 6

4.2.3Panel ____________________________________________________________ 8

4.3Sample Rotor and Fan Wheel _________________________________ 10

4.4Heating Element ___________________________________________ 12

4.5Chamber Lid _______________________________________________ 14

4.6Rotor _____________________________________________________ 16

4.6.1Stepper Motor Rotor ______________________________________________ 16

4.6.2Fan Motor _______________________________________________________ 18

4.6.3Measuring Chamber ______________________________________________ 20

4.7Fluorimeter Drive ___________________________________________ 22

4.8Lightbarriers and Sensors ___________________________________ 24

4.8.1Location ________________________________________________________ 24

4.8.2Rotor Lightbarrier ________________________________________________ 25

4.8.3Interlock Lightbarrier _____________________________________________ 25

4.8.4Fluorimeter Lightbarrier ___________________________________________ 26

4.8.5MonitorTemperature Sensor _______________________________________ 26

4.8.6Control Temperature Sensor _______________________________________ 27

4.8.7ExcessTemperature Switch ________________________________________ 27

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4.9Power Supply ______________________________________________ 28

4.9.1Location ________________________________________________________ 28

4.10PCBs _____________________________________________________ 30

4.10.1Location ________________________________________________________ 30

4.10.2PCB Controller und Battery ________________________________________ 31

4.10.3PCB Driver ______________________________________________________ 31

4.10.4Status Board ____________________________________________________ 31

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4 Mechanics

4.1Overview

Fig. 160798k1

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4.2Disassembly of the Housing Parts

4.2.1Housing Lid

4.2.1.1 Location

Fig. 140798k2

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