FV10i-LIV
86(5b60$18$/
[HARDWARE]
FLUOVIEW
FV10i-LIV
Confocal Laser Scanning Biological Microscope
Note
؞This instruction manual is for the Olympus Confocal Laser Scanning Microscope Model
FLUOVIEW FV10i-LIV.
To ensure the safety, obtain optimum performance and familiarize yourself fully with the
use of this microscope, we recommend that you study this manual thoroughly before
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MANUAL [SAFETY] together with this manual.
؞ For the operating procedures for observation and acquisition, please also read separate
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؞ Retain this instruction manual in an easily accessible place near the work desk for future
reference.
A X 8 0 1 7 [TYPE 3]
CONTENTS
FLUOVIEW FV10i-LIV
IMPORTANT
1 SYSTEM AND MAIN CONTROL
2 ASSEMBLY
1 Connecting the Cables ................................................................................................................................................................ 5
2 Connecting the Tubes .............................................................................................................................................................. 6,7
3 Preparation before Placing the Specimen ................................................................................................. 8,9
4 Placing the Specimen ............................................................................................................................................................ 9,10
3 ROUTINE MAINTENANCE
1 Cleaning the Objective.............................................................................................................................................................. 11
2 Refilling/Draining Water in the Incubator ....................................................................................................... 11
1
2-4
5-10
11,12
3 Refilling Water in the Objective Water Tank ............................................................................................... 11
4 Draining Water from the Water Tray ....................................................................................................................... 11
5 Disinfection and Sterilization ............................................................................................................................................ 12
PROPER SELECTION OF THE POWER SUPPLY CORD .................................................................................. 13,14
IMPORTANT
For safety precautions, please refer to attached USER’S MANUAL
[ SAFETY ].
Getting Ready
1
1. Always use ultrapure water with the incubator and water tank.
2. Four forms of specimen (containers) can be used including a
cover glass chamber (8 compartments) and well slide (8 compartments). The standard thickness of the cover glass is
0.17 mm, but the range from 0.13 to 0.21 mm is acceptable.
3. After starting up the system, reserve a warm-up period of about 3 hours (When the use environment temperature is 25°C)
until the internal temperature of the incubator stabilizes. When the setting of the internal temperature of the incubator is
not necessary, hold the main switched pressed (for 3 seconds), and the system can be used right away.
4. The operating period warranted for the LD light source is the shorter period of 2000 hours or one (1) year after the delivery
inspection.
5. Depending on the fluctuation of the temperature in the usage environment, a drift may occur in the X, Y or Z direction.
6. Do not apply strong impact to the equipment.
7. Install this product on the flat (Tilt : less than 2°) and durable table.
8. Reserve clearances of 15 cm or more in front of the air inlet and outlet.
9. Be sure to clean or replace as required the filter connected to the water tank that stores the ultrapure water supplied to the
objective front.
10. Refill the ultrapure water to be supplied to the objective front whenever the display shows the warning message.
11. When a specimen holder for
specimens in the unused specimen positions.
12. If water drops are left on the inner side of the top cover of the
image may be degraded.
13. When a high-power objective is switched to a low-power objective, the image quality may be degraded if water is
attached at the bottom side of the specimen.
14. The Z-stack image can be obtained only in the direction from the position distant to the specimen toward the position
closer to it.
15. When the equipment is not to be used for a long period (more than 2 weeks), be sure to drain ultrapure water from the
tank for the ultrapure water supplied to the objective front.
16. If the inside of the water pump is dried, the failure may occur in the product performances. Refill the water tank with water
at least once a year (P. 8, 11) and drain water from the water pump (P. 9).
17. Long-period oscillations (20 Hz or less) may affect the image quality.
18. Oscillations may be noticeable during zooming. This is due to the fan of the incubator and can be reduced by increasing
the Karman integration count.
19. When disposing of the microscope, be sure to observe the regulations or rules of your local government.
35 mm dish is used and the number of specimens is 1 or 2, be sure to place dummy
35 mm glass-bottom dish, slide glass (76 mm x 26 mm),
35 mm glass-bottom dish, the quality of the transmitted
1
Caution
2
If the equipment is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the
equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
1
SYSTEM AND MAIN CONTROLS
Main Unit: FV10C-W3
FLUOVIEW FV10i-LIV
Cover
Close lever
Water tank cover
Water tank for
immersed objective
(Approx. 200 ml) is
built in.
Water stop valve
Water tray
View when the water tank cover is
opened
Incubator temperature
· Blinks during warmup.
· “- -” is displayed when
the temperature setting is OFF.
CO2 inlet port
Cover lock buttons
Push the botton to open the cover.
When setting the sub-switch to ON
· Press it shortly to start up the main unit with the heater
ON.
· Press and hold it until a short beep is generated (about
3 seconds) to start up the main unit with the heater
OFF.
Sub-switch OFF
· Press and hold for 3 sec.
}Status LEDs when both the main and sub switches are ON.
LEDs
Left Right
Green
blinking
Green
lighting
Green
lighting
· The left LED lights in red when the main switch is set to “ ”
(ON).
· Both the left LED (green) and right LED (orange) blink when
the cover is open.
Orange
lighting
Orange
lighting
Orange
blinking
Laser Software
Initializing
Standby Not run
Standby Running
indicator
Sub-switch
Status LEDs
Water level
window
Water inlet
With a cap and filter.
2
Specimen Holders
1. Specimen holder for slide glass: FV10C-HOS-2
Specimen
stoppers
Positioning
hole
Map area indicator
Holder handle
3. Specimen holder for 35 mm dish: FV10C-HOD3
2. Specimen holder for chamber: FV10C-HOC
Chamber
stopper
Positioning hole
4. Specimen holder for culture pod: FV10C-HOP
Culture solution
outlet
Top cover clamping
screws
x 4 pcs.
Positioning
hole
Dish stopper
CAUTION
To keep the internal temperature and humidity of
the incubator stable, be sure to place dummy
specimens in the unused specimen positions
when the number of specimens used is 1 or 2.
Culture solution
inlet
Positioning holes
5. Specimen holder for well slide: FV10C-HOW
Slide glass
stopper
Holder handle
Positioning
hole
3
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