Confocal Laser Scanning
Biological Microscope
FV1000
FLUOVIEW
FLUOVIEW—Always Evolving
1
FLUOVIEW–—From
Olympus is Open
FLUOVIEW—More Advanced than Ever
The Olympus FLUOVIEW FV1000 confocal laser scanning microscope delivers
efficient and reliable performance together with the high resolution required for
multi-dimensional observation of cell and tissue morphology, and precise
molecular localization. The FV1000 incorporates the industry’s first dedicated
laser light stimulation scanner to achieve simultaneous targeted laser stimulation
and imaging for real-time visualization of rapid cell responses. The FV1000 also
measures diffusion coefficients of intracellular molecules, quantifying molecular
kinetics. Quite simply, the FLUOVIEW FV1000 represents a new plateau, bringing
“imaging to analysis.”
Olympus continues to drive forward the development of FLUOVIEW
microscopes, using input from researchers to meet their evolving demands and
bringing “imaging to analysis.”
Quality Performance with Innovative Design
FV10i
2
Imaging to Analysis
ing up New Worlds
From Imaging to Analysis
FV1000
Advanced Deeper Imaging with High Resolution
FV1000MPE
Advanced FLUOVIEW Systems Enhance the Power of
Your Research
Superb Optical Systems Set the Standard
for Accuracy and Sensitivity.
Two types of detectors deliver enhanced accuracy and sensitivity,
and are paired with a new objective with low chromatic aberration,
to deliver even better precision for colocalization analysis.
These optical advances boost the overall system capabilities and raise
performance to a new level.
Imaging, Stimulation and Measurement—
Advanced Analytical Methods for Quantification.
Now equipped to measure the diffusion coefficients of intracellular
molecules, for quantification of the dynamic interactions of molecules
inside live cell.
FLUOVIEW opens up new worlds of measurement.
Evolving Systems Meet the Demands of Your Application.
Upgradeable system with optional hardware and software to meet
the demands of your research.
3
4
Laser combiner/Fiber
Diode Laser
Greater stability, longer service life and
lower operating cost are achieved using
diode lasers.
Laser Feedback Control
Scanner unit is equipped with laser power
monitor for feedback control enhancing
stable laser output.
Laser Compatibility
Diode laser :
405 nm, 440 nm, 473 nm, 559 nm, 635 nm
Gas laser :
Multi-line Ar laser (458 nm, 488 nm, 515 nm)
HeNe(G) laser (543 nm)
Broadband Fiber
Broadband fiber connection for 405–635 nm
lasers, to achieve an ideal point light source
with minimal color shift and position shift
between images.
LD405
LD473
LD559
LD635
AOTF
Grating
Barrier filter
Confocal pinhole
Grating
AOTF
Laser combiner
Main scanner
Broadband fiber *
Broadband fiber
Excellent Precision, Sensitivity and Stability.
FLUOVIEW Enables Precise, Bright Imaging with Minimum Phototox
Two versions available.
•Single fiber-type combiner is used for
main scanner FV1000 with up to six lasers,
ranging from 405 to 635 nm.
•Dual fiber-type combiner is used for laser
light stimulation with main and SIM
scanner FV1000.
Laser Combiner
Scanners/Detection
High Sensitivity Detection System
High-sensitivity and high S/N ratio optical
performance is achieved through the
integration of a pupil projection lens, use
of a high sensitivity photomultiplier tube
and an analog processing circuit with
minimal noise. Enables high S/N ratio
image acquisition with minimal laser power
to reduce phototoxicity.
Up to Four PMT Channels
Three integrated confocal PMT detectors,
and optional module with fourth confocal
PMT expandable up to four PMT channels.
Two Versions of Light Detection System
• Spectral detection for high-precision
spectroscopy with 2 nm resolution.
• Filter detection equipped with high
quality filter wheels.
Spectral Scanning Unit
Filter Scanning Unit
5
Technology / Hardware
IX81
PMT
PMT
PMT
Galvanometer
scanning mirrors
Galvanometer
scanning mirrors
UIS2 objectives
Specimen
Pupil
projection
lens
SIM Scanner *
Microscope
icity.
Optical System
Motorized Microscopes
Compatible with Olympus IX81 inverted
microscope, BX61WI focusing nosepiece
and fixed-stage upright microscope, and
BX61 upright microscope.
Samples and Specimens
Supports a Wide Range of Samples and
Specimens
Tissue culture dishes, slide chambers,
microplates and glass slides can be used
with live cells and fixed specimens.
BX61
UIS2 Objectives
Olympus UIS2 objectives offer worldleading, infinity-corrected optics that
deliver unsurpassed optical performance
over a wide range of wavelengths.
High S/N Ratio Objectives with
Suppressed Autofluorescence
Olympus offers a line of high numerical
aperture objectives with improved
fluorescence S/N ratio, including
objectives with exceptional correction for
chromatic aberration, oil- and waterimmersion objectives, and total internal
reflection fluorescence (TIRF) objectives.
6
* Option
Spectral Based Detection
Flexibility and High Sensitivity
Spectral detection using gratings
for 2 nm wavelength resolution
and image acquisition matched to
fluorescence wavelength peaks.
User adjustable bandwidth of
emission spectrum for acquiring
bright images with minimal crosstalk.
Precise Spectral Imaging
The spectral detection unit uses a grating method that offers
linear dispersion compared with prism dispersion. The unit
provides 2 nm wavelength resolution to high-sensitivity
photomultiplier tube detectors. Fluorescence separation can be
achieved through unmixing, even when cross-talk is generated
by multiple fluorescent dyes with similar peaks.
496 500 504 508 512 516 520 524
Wavelength
Intensity
528 532 536 540 544 548 552
400
600
800
1,000
1,200
1,400
1,600
1,800
2,000
2,200
2,400
2,600
EGFP (dendrite) — EYFP (synapse)
XYλ
Wavelength detection range: 495 nm–561 nm in
2 nm steps
Excitation wavelength: 488 nm
Courtesy of: Dr. Shigeo Okabe
Department of Anatomy and Cell Biology,
Tokyo Medical and Dental University
EGFP–EYFP Fluorescence Separation
EYFP
EGFP
Two Versions of Light Detection System that Set New Standards
for Optical Performance.
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Filter Based Detection
Enhanced Sensitivity
Three-channel scan unit with detection system featuring hard
coated filter base. High-transmittance and high S/N ratio optical
performance is achieved through integration of a pupil projection
lens within the optics, the use of a high sensitivity photomultiplier
and an analog processing circuit with minimal noise.
High-Performance Filters Deliver Outstanding Separation
Special coatings deliver exceptionally sharp transitions to a
degree never achieved before, for acquisition of brighter
fluorescence images.
Transmittance (%)
100
80
60
40
20
0
480
500 520 540 560 580
Conventional mirror unit High-performance mirror unit
DM488/543/633 Comparison
600 620 640 660 680 700
Wavelength (nm)
Technology / Hardware
SIM (Simultaneous) Scanner Unit
Combines the main scanner with a dedicated laser light
stimulation scanner for investigating the trafficking of fluorescentlabeled molecules and marking of specific live cells.
Simultaneous Laser Light Stimulation and Imaging
Performs simultaneous laser light stimulation and imaging to
acquire images of immediate cell responses to
stimulation in photobleaching experiments.
Modifiable Stimulation Area During Imaging
The stimulation area can be moved to a different position on the
cell during imaging, providing a powerful tool for photoactivation
and photoconversion experiments.
Wide Choice of Bleaching Modes
Various scan modes can be used for both the observation area
and stimulation area. Enables free-form bleaching of designated
points, lines, free-lines, rectangles and circles.
SIM Scanner Unit for Simultaneous Laser Light Stimulation and
Imaging.
Branching of laser in
laser combiner.
Lasers are used for both imaging
and laser light stimulation.
LD405
LD635
AOTF
AOTF
LD473
LD559
Multi-Purpose Laser Combiner
All lasers can be used for both Imaging and laser light stimulation.
LD405 / LD635 / AOTF / AOTF / LD473 / LD559
Laser Sharing with Main Scanner
Dual fiber laser combiner provides laser sharing between the SIM
scanner and main scanner, eliminating the need to add a
separate laser for stimulation.
Unique "Tornado" Scanning for Efficient Bleaching
Conventional raster scanning does not always complete
photobleaching quickly. Tornado scanning greatly improves
bleaching efficiency by significantly reducing unnecessary
scanning.
*Tornado scanning only available for SIM scanner.
Tornado scanning
Superfluous scanning areas.
ROI (region of interest)
scanning
ROI (region of interest) scanning.
Tornado scanning.
Cell membrane stained with DIO, and subjected to both
conventional ROI and tornado scanning.
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Low Chromatic Aberration Objective
Best Reliability for Colocalization Analysis
A new high NA oil-immersion objective minimizes chromatic
aberration in the 405–650 nm region for enhanced imaging
performance and image resolution at 405 nm. Delivers a high
degree of correction for both lateral and axial chromatic
aberration, for acquisition of 2D and 3D images with excellent
and reliable accuracy, and improved colocalization analysis. The
objective also compensates for chromatic aberration in the near
infrared up to 850 nm.
New Objective with Low Chromatic Aberration Delivers
World-Leading Imaging Performance.
NEW
NEW
Low Chromatic PLAPON60xOSC
Aberration Objective Magnification: 60x
NA: 1.4 (oil immersion)
W.D.: 0.12 mm
Chromatic aberration compensation range: 405–650 nm
Optical data provided for each objective.
3D image
Tubulin in Ptk2 cells labeled with
two colors (405 nm, 635 nm) and
compared.
Improved Flatness and Resolution at 405 nm
Better flatness reduces the number of images for tiling.
g
UPLSAPO60xO
Chromatic Aberration Comparison for PLAPON 60xOSC and
UPLSAPO 60xO
Performance Comparison of PLAPON 60xOSC and UPLSAPO 60xO
PLAPON60xOSC
Axial chromatic
aberration (Z direction)
Compared for PSF fluorescent
beads (405 nm, 633 nm).
Lateral chromatic
aberration (X-Y direction)
Compared for PSF fluorescent
beads (405 nm, 488 nm, 633
nm).
*Chromatic aberration values are design values and are not guaranteed values.
Lateral and Axial Chromatic Aberration
Small Degree of
Chromatic Aberration
Large Degree of
Chromatic Aberration
Axial chromatic
aberration
(Z direction).
Lateral chromatic
aberration (X-Y
direction at FN6).
Approx.
0.5 µm
Approx.
0 µm
Approx. 0.1 µm Approx. 0.2 µm
Objective
PLAPON60xOSC UPLSAPO60xO
Flatness Comparison Image at 1x Zoom
9
0.5
Lateral Chromatic Aberration
UPLSAPO60xO
0.0
Focal plane (µm)
-0.5
PLAPON 60xOSC
450400
500 550 600
Wavelen
650
th (nm)
Technology / Hardware
Switchable between Confocal and TIRFM Imaging
Switchable between confocal and TIRFM imaging for localization
of proteins on the cytoplasmic membrane surface and acquisition
of sectioning images within cells.
Software Control of TIRF Illumination
Built-in laser provides TIRF illumination. Software can be used to
tune the angle of incidence of excitation light and calculates the
penetration depth of the evanescent wave based on the TIRF
objective used.
High-Numerical Aperture Objectives for TIRF Illumination
A line of high-numerical aperture (NA) objectives is available for
TIRF illumination.
TIRFM (Total Internal Reflection Fluorescence Microscope) System
FV1000MPE Multiphoton Excitation System
Brighter and Deeper Imaging with Finer Resolution
The FV1000 is upgradeable to multiphoton excitation capability
by adding a dedicated laser and multiphoton optical system.
Optical design is optimized for multiphoton principles for brighter
imaging of features deep within living specimens, at higher
resolutions than previously possible.
Special Multiphoton Objective with Outstanding
Brightness and Resolution
Olympus offers a high NA water-immersion objective designed
for a wide field of view, with improved transmittance at nearinfrared wavelengths. A correction collar compensates for
spherical aberration caused by differences between the refractive
indices of water and specimens, forming the optimal focal spot
even in deep areas, without loss of energy density. The objective
is designed to collect scattered light over a wide field of view for
maximum image brightness.
Multiphoton Laser Light Stimulation
Adding a multiphoton laser to the SIM scanner enables
multiphoton laser light stimulation or uncaging confined to the
focal volume.
Exceptional Resolution for Imaging of Cytoplasmic Membrane
and Areas Deep Within Living Specimen.
GFP—Pak—K298A in HeLa cells.
Courtesy of Dr.J M Dong of sGSK-NRP laboratory, Singapore
LSMTIRFM
APON60xOTIRF
NA : 1.49 (oil immersion)
WD: 0.1 mm
Apo100xOHR
NA : 1.65 (oil immersion)
WD: 0.1 mm
(Customized cover glass and
immersion oil)
UAPON100xOTIRF
NA : 1.49 (oil immersion)
WD: 0.1 mm
UAPON150xOTIRF
NA : 1.45 (oil immersion)
WD: 0.08 mm
NEW
NEW
NEW
NEW
NEW
NEW
XLPLN25xWMP
Magnifications : 25x
NA : 1.05 (water immersion)
W.D. : 2.0 mm
3-dimensionally constructed images of neurons expressing EYFP in the cerebral neocortex of a
mouse under anesthesia.
Courtesy of:
Hiroaki Waki, Tomomi Nemoto, and Junichi Nabekura
National Institute for Physiological Sciences, National Institutes of Natural Sciences, Japan
* The FLUOVIEW FV1000MPE is a class 4 laser product.
10
User-Friendly Software to Support Your Research.
Time Controller
Precisely synchronizes different
experimental protocols including FRAP,
FLIP and FRET by acceptor photobleaching and time-lapse. Save and open
settings for later use.
Wide Choice of Scanning Modes
Several available scanning modes
including ROI, point and high-speed
bidirectional scanning.
Configurable Excitation Laser
Power
Easily adjust the optimum laser power for
each specimen (live cells and fixed
specimens).
Image Acquisition by Application
User-friendly icons offer quick access to
functions, for image acquisition according
to the application (XYZ, XYT, XYZT, XYλ,
XYλT).
Configurable Emission
Wavelength
Select the dye name to set the optimal
filters and laser lines.
11
Technology / Hardware
Re-Use Function
Open previously configured scanning
conditions and apply them to new or
subsequent experiments.
Help Guide
Comprehensive help guide describes the
functions and usage for each command,
and overall sequence of operations.
Multi Stimulation Software
Configure multiple stimulation points and
conditions for laser light stimulation
synchronized with imaging, for detailed
analysis of the connectivity of cells within
the stimulation area.
Multi-Area Time-Lapse Software
Multi-Area Time-Lapse
Software control of the motorized XY
stage enables multiple measurement
points in glass slides, 35 mm dishes or
individual microplate wells. Repeated
imaging of multiple cells improves the
statistical power of time-lapse
experiments.
Mosaic Imaging
A motorized XY stage is programmed with
the use of a high-magnification objective
to acquire continuous images from
adjacent fields of view, to assemble a
single, high resolution image covering a
wide area. Three-dimensional images can
also be assembled using XYZ acquisition.
For analysis of intracellular molecular
interactions, signal transduction and other
processes, by determining standard
diffusion coefficients. Supports a wide
range of diffusion analysis using point
FCS, RICS and FRAP.
Diffusion Measurement Package
Optional Software with Broad Functionality.
12
Broad Application Support and Sophisticated Experiment
Control.
Measurement
Light Stimulation
Multi-
Dimensional
Time-Lapse
FRET
Colocalization
3D Mosaic
Imaging
Multi-Color
Imaging
3D/4D
Volume
Rendering
13
Application
15,000 20,000 30,00025,000
Time (ms)
35,000 40,000
200
400
600
800
1,000
1,200
1,400
1,600
1,800
2,000
2,200
2,400
Intensity
CH1
CH2
CH1
CH2
Measurement
Diffusion measurement and molecular interaction
analysis.
Light Stimulation
FRAP/FLIP/Photoactivation/Photoconversion/Uncaging.
Multi-Dimensional Time-Lapse
Long-term and multiple point.
3D Mosaic Imaging
High resolution images stitched to cover a large area.
Multi-Color Imaging
Full range of laser wavelengths for imaging of diverse
fluorescent dyes and proteins.
3D/4D Volume Rendering
One-click 3D/4D image construction from acquired
XYZ/T images.
Change the angle of 3D image with a single click.
Colocalization
Configurable threshold values for fluorescence
intensities on the scatterplot.
Accurate colocalization statistics and visualization of
colocalized area on image.
FRET
Configuration wizard simplifies the setting of FRET
experimental procedures.
Optimal laser excitation wavelengths for CFP/YFP
FRET.
Image of variations in calcium concentration of HeLa cells
expressing YC3.60 when stimulated with histamine.
Reference:
Takeharu Nagai, Shuichi Yamada, Takashi Tominaga, Michinori
Ichikawa, and Atsushi Miyawaki 10554-10559, PNAS, July 20,
2004, vol. 101, no.29
14
This optional software module enables data acquisition and analysis to investigate the molecular interaction
and concentrations by calculating the diffusion coefficients of molecules within the cell.
Diverse analysis methods (RICS/ccRICS, point FCS/point FCCS and FRAP) cover a wide range of molecular
sizes and speeds.
Diffusion Measurement Package
105
105
125
Pixels
Pixels
125
130
130
0
0.5
1
1.5
p
RICS
FRAP
00
Capable range
of measurement
00
00< 0.1< 0.01
001
Small molecules
Proteins
Diffusion of
p
plex
,
Protein
g
Lateral diffusion
e
RICS—Raster Imaging Correlation Spectroscopy
Raster image correlation spectroscopy (RICS) is a new method for analyzing the diffusion
and binding dynamics of molecules in an entire, single image. RICS uses a spatial
correlation algorithm to calculate diffusion coefficients and the number of molecules in
specified regions.
Cross correlation RICS (ccRICS) characterizes molecular interactions using fluorescentlabeled molecules in two colors.
FRAP Analysis
The Axelrod analytical algorithm is installed as a FRAP analysis method. The algorithm is used to calculate diffusion coefficients and the
proportions of diffusing molecules.
point FCS—Point scan Fluorescence Correlation Spectroscopy
point scan fluorescence correlation spectroscopy (point FCS) analyzes intensity
fluctuations caused by diffusion or binding/unbinding interactions of a protein complex.
point FCS uses an auto correlation function to carry out operations on fluorescence
signals obtained by continuous scanning of a single pixel on the screen.
point scan fluorescence cross-correlation spectroscopy (point FCCS) analyzes the
fluctuation of fluorescent-labeled molecules in two colors. The coincidence of fluctuations
occurring in two detection channels shows that the two proteins are part of the same
complex.
point FCS and point FCCS can now be performed with a standard detector, eliminating
the need for a special high-sensitivity detector.
Analytical methods
according to molecule
diffusion speeds
15
in solution
in solution
roteins
in cell
in cell membran
traffickin
Molecular com
formation
aggregation
> 1
~ 1
1 ~ 1
<< 0.
oint FCS
Application/ Molecular Interaction Analysis
0 µs
0 ms
0 ms
1 ms
2 ms
3 ms
4 ms
n ms
10 µs 20 µs 30 µs 40 µs 50 µs n µs
Molecule size
Small Large
Spatial Correlation Algorithm
When the spatial correlation algorithm is applied between pixels, a higher correlation
is obtained as the speed of movement of the molecule nears the scanning speed.
When calculating the spatial correlation in the X-direction, because the scanning
speed in the X-direction is fast, a higher correlation is obtained for fast-moving
molecules than for slow-moving molecules. When the scanning speed in the Ydirection is slow, a higher correlation is obtained for slow-moving molecules. RICS
using LSM images scans in both X- and Y-directions, so it can be used to analyze
the movements of a wide range of molecules, both fast and slow.
Scan in X-Axis Direction
Scan in Y-Axis Direction
RICS Application and Principles
RICS Principle
Molecules of different sizes diffuse at different speeds within
cells. Small molecules move faster, compared with large
molecules that move relatively slowly. The FV1000 acquires
information on the movement of these diffusing fluorescentlabeled molecules as image data, together with morphological
information about the cell. The image data obtained for each
pixel was sampled at different times, so the data for each pixel is
affected by the passage of time, in addition to its spatial XY
information. By analyzing this image data with a new statistical
algorithm for spatial correlation, the diffusion coefficients and
molecule counts can be calculated for molecules moving within
the cell.
RICS Analysis Method
Theoretical Formula Used
for Fitting Calculation
Results of Analysis
(diffusion coefficient and
molecule count)
LSM Image Spatial Correlation
16
At cytoplasmic membrane
Diffusion coefficient D =0.98 µm2/s
In cytoplasm
Diffusion coefficient D =3.37 µm2/s
Sample image:
HeLa cells expressing EGFP fusion PKC (after PMA stimulation)
Comparison of Diffusion Coefficients for EGFP Fusion Proteins Near to Cell Membranes and In Cytoplasm
RICS can be used to designate and analyze regions of interest
based on acquired images.
EGFP is fused at protein kinase C (PKC) for visualization, using
live cells to analyze the translocation with RICS. The diffusion
coefficient close to cell membranes was confirmed to be lower
than in cytoplasm, after stimulation with phorbol myristate
acetate (PMA). This is thought to be from the mutual interaction
between PKC and cell membrane molecules in cell membranes.
In addition to localization of molecules, RICS analysis can
simultaneously determine changes in diffusion coefficient, for
detailed analysis of various intracellular signaling proteins.
Laser Light Stimulation
The SIM scanner system combines the main scanner with a laser light stimulation scanner.
Control of the two independent beams enables simultaneous stimulation and imaging, to capture reactions
during stimulation.
Multi-stimulation software is used to continuously stimulate multiple points with laser light for simultaneous
imaging of the effects of stimulation on the cell.
FLIP—Fluorescence Loss in Photobleaching
Fluorescence loss in photobleaching (FLIP) combines imaging with continuous bleaching of a specific region to observe the diffusion of a
target protein within a cell. The changes in the image over time make it possible to observe the location of structural bodies that inhibit
the diffusion of the molecule.
FRAP—Fluorescence Recovery after Photobleaching
Exposure of fluorescent-labeled target proteins to strong laser light causes their fluorescence to fade locally. Fluorescence recovery after
photobleaching (FRAP) is used to observe the gradual recovery of fluorescence intensity caused by protein diffusion from the area
surrounding the bleached region. By examining the resulting images, it is possible to characterize the diffusion speed of the molecule,
and the speed of binding and release between the molecule and cell structures.
Example: Fluorescence recovery without interactions
If the protein can freely diffuse, the bleached region recovers
its fluorescence at a high speed due to Brownian motion.
Example: Fluorescence recovery with interactions
If the protein is strongly bound to a structure or forms part of a
large protein complex, the bleached region recovers its
fluorescence at a slower rate relative to the unbound state.
Time
Fluorescent intensity
Time
Fluorescent intensity
Specimen: HeLa cell, GFP (free), 488 nm excitation (multi-argon laser)
Image acquisition time: 100 ms/ bleach time: 100 s continuously, 405 nm bleaching
0
0
200
400
600
800
1,000
1,200
1,400
1,600
1,800
2,000
2,200
2,400
2,600
2,800
3,000
10,000 20,000 30,000 40,000 50,000
Time (ms)
60,000 70,000 80,000 90,000 100,000
Intensity
Specimen: Hippocampal neurons, Shank-GFP stain, 488 nm excitation (multi-argon laser)
Image acquisition time: 100 ms Bleach time: 80 ms, 488 nm excitation (Sapphire 488 laser)
Data courtesy of: Dr. Shigeo Okabe
Department of Anatomy and Cell Biology, Tokyo Medical and Dental University
0
250
300
350
400
450
500
550
600
650
750
700
10,000 20,000 30,000 40,000 50,000
Time (ms)
60,000 70,000 80,000
Intensity
17
Application/ Molecular Interaction Analysis
Photoconversion
The Kaede protein is a typical photoconvertible protein, which is a specialized fluorescent protein that changes color when exposed to
light of a specific wavelength. When the Kaede protein is exposed to laser light, its fluorescence changes from green to red. This
phenomenon can be used to mark individual Kaede-expressing target cells among a group of cells, by exposing them to laser light.
450 nm laser light
Kaede-expressing astroglia cells are stacked on the Kaede-expressing neurons. By illuminating two colonies with a 405 nm laser, the Kaede color can
be photoconverted from green to red. The glial cells in contact with the neurons are observed while they are forming colonies and extending their
processes, and the nuclei of these colonies can also be observed. The SIM scanner FV1000 makes it easy to change cell colors from green to red while
conducting an observation, and to control neutral colors between red and green.
Data courtesy of: Dr. Hiroshi Hama, Ms. Ryoko Ando and Dr. Atsushi Miyawaki, RIKEN Brain Science Institute Laboratory for Cell Function Dynamics
Before Stimulation After Stimulation
405 nm
405 nm
Caged-Glutamate
Fluorescent calcium indicator Fluo-3 in HeLa cells. Image acquisition at 1-second intervals
Using the caged compound Bhcmoc-Glutamate, an increase in calcium ion concentration inside the cell can be observed in response to
glutamate stimulation, released via 405 nm laser illumination.
Data courtesy of:
Dr. Hiroshi Hama, Dr. Atsushi Miyawaki, RIKEN Brain Science Institute Laboratory for Cell Function Dynamics
Caged compound Bhcmoc-Glutamate presented by Dr. Toshiaki Furuta, Department of Science, Toho University
0 5,000 10,000 20,00015,000 30,00025,000 35,000
Time (ms)
40,000 50,00045,000 55,000
500
600
700
800
900
1,000
1,100
1,200
1,300
1,400
1,500
1,600
1,700
1,800
1,900
2,000
Intensity
18
Uncaging
A 405nm laser is optional for uncaging with the SIM scanner system. Caged compounds can be uncaged point-by-point or within a
region of interest, while the main scanner of the FV1000 captures images of the response with no time delay.
Multi-Point Laser Light Stimulation
Using multi-stimulation software, the user can configure continuous laser light stimulation of multiple points with simultaneous imaging,
which is effective for applications such as uncaging experiments involving laser light stimulation of several spines in neurons.
Significantly Improved Long Time-Lapse Throughput
Equipped with motorized XY stage for repeated image acquisition from multiple points scattered across a wide area. The system
efficiently analyzes changes over time of cells in several different areas capturing, large amounts of data during a single experiment to
increase the efficiency of experiments. Microplates can be used to run parallel experiments, which significantly improves throughput for
experiments that require long-term observation.
Focal Plane 1
Focal Plane 2
Focal Plane 3
Focal Plane 4
Point 1
Point 2
Point 3
Point 4
Point 5
Point 6
Multi-Point Time-Lapse Software
The FV1000 can be used for ideal multi-dimensional time-lapse imaging during confocal observation,
using multi-area time-lapse software to control the motorized XY stage and focus compensation.
Multi-Dimensional Time-Lapse
P1
P2
P3
P4
P5
Maintain Cell Activity Over A Long Period
Proprietary CO2 incubator control keeps the environment inside the tissue culture dish completely stable. The environment is precisely
maintained at 37°C with 90% humidity and 5% CO
2 concentration.
ZDC
Baseline focal plane
IR Laser for focal
plane detection
Offset
Scanning
unit
Supports repeated image
acquisition from multiple areas in
a single microplate well.
0 s 1000 s 2000 s 3000 s 5000 s 6000 s 7000 s4000 s
Human lymphoblast cells TK6
Courtesy of: Masamitsu Honma, Dir.
Biological Safety Research Center Div. of Genetics and Mutagenesis I, National Institute of Health Sciences
19
Focal Drift Compensation for Long Time-Lapse Imaging
The IX81-ZDC Zero Drift Compensation system corrects loss of focus caused by temperature changes around the microscope and other
factors during long time-lapse observation. The thermal drift compensation eliminates the need to take images at several Z planes,
minimizing live cell exposure to irradiation.
Objective
focal
plane
Set target
observation
plane
as offset.
Over time, the
objective focal
plane drifts from
the observation
plane.
Laser detects
the glass
surface before
imaging.
Immediately
returns to initial
offset plane,
for focal drift
compensation.
Application/ Molecular Interaction Analysis
3D Mosaic Imaging
Mosaic imaging is performed using a high-magnification objective to acquire continuous 3D (XYZ) images
of adjacent fields of view using the motorized stage, utilizing proprietary software to assemble the
images. The entire process from image acquisition to tiling can be fully automated.
Automated from 3D Image Acquisition to Mosaic Imaging
Multi-area time-lapse software automates the process
from 3D image acquisition (using the motorized XY
stage) to stitching. The software can be used to easily
register wide areas, and the thumbnail display
provides a view of the entire image acquired during
the mosaic imaging process.
Coordinate Information
Thumbnail
CNS markers in normal mice
Objective : PLAPON60x
Zoom : 2x
Image acquisition numbers (XY): 32 x 38, 48 slices for each image
Courtesy of: Dr. Mark Ellisman PhD, Hiroyuki Hakozaki, MS Mark Ellisman
National Center for Microscopy and Imaging Research (NCMIR),
University of California, San Diego
Mosaic Imaging for 3D XYZ Construction
Composite images are quickly and easily prepared using the stitching function, to form an image over a wide area. 3D construction can
also be performed by acquiring images in the X, Y and Z directions. Tiled images can be enlarged in sections without losing resolution.
20
Expandability to Support Diverse Application.
Application Standard Functions Optional Functions
Molecular interaction and Intracellular diffusion measurement
molecular concentration Calculation of diffusion coefficients for intracellular molecules, and analysis of
analysis
—
molecular binding and changes in molecular density.
Supports a wide range of methods (RICS/ccRICS, point FCS/point FCCS and
FRAP).
Software Required: Diffusion measurement package
Laser Light stimulation Acquires images while rapidly switching SIM scanner system
the built-in laser between imaging and Performs simultaneous imaging and laser light stimulation. Provides detailed
laser light stimulation. settings for laser light stimulation including position and timing.
Features tornado scanning for high- Features tornado scanning for high-efficiency bleaching using laser light
efficiency bleaching using laser light stimulation.
stimulation. Equipment Required: SIM scanner, laser combiner (dual fiber version)
Multi-point laser light stimulation system
Register multiple points for laser light stimulation, and program the respective
stimulation order, stimulation time and type of stimulation (continuous laser light
or pulse laser light).
Software Required: Multi-stimulation software
Multi-dimensional Long time-lapse system
time-lapse imaging Microscopes equipped with zero drift compensation (ZDC) acquire each image at
a set focus plane. The microscope CO2 incubator maintains cell activity for a long
period for continuous imaging.
— Equipment Required: IX81-ZDC microscope, CO2 incubator
Multi-point scanning system
Register multiple points for repeated image acquisition. Efficiently observe multiple
cells in parallel on 35-mm dishes, microplates or chamber slides.
Software and Equipment Required:
Multi-area time-lapse software, motorized XY stage**
3D mosaic imaging 3D mosaic imaging system
Continuous imaging of adjacent fields of view and mosaic imaging to form a
— composite image.
Acquisition of adjacent Z-series images for 3D mosaic imaging.
Software and Equipment Required:
Multipoint time-lapse software, motorized XY stage**
TIRFM TIRFM imaging
Uses the laser from the laser combiner to provide evanescent illumination, for
—
imaging the movement of molecules near the glass surface, such as cell
membranes and adhesion factors.
Software and Equipment Required: TIRFM unit*, TIRF objective, highsensitivity CCD camera**, CCD camera control software**
FRET Provides FRET analysis functions. CFP-YFP FRET
Diode laser offers exceptional stability Ratio imaging and sensitized emission.
and long life. Available 440 nm diode laser is optimized for CFP-YFP FRET experiments
Supports FRET efficiency methods.
measurements using acceptor Diode laser offers exceptional stability and long life.
photobleach method. Equipment Required: LD 440 nm Laser
Multi-color imaging Three-channel detector for Imaging blue dyes
simultaneous acquisition of Available 405-nm laser for image acquisition of multi-stained samples labeled with
fluorescence images from three V-excitation fluorescent dyes such as DAPI, Hoechst and Alexa 405.
different dyes. Equipment Required: LD 405 nm laser
Sequential mode for acquisition of
Simultaneous four-color imaging
fluorescence images without cross-talk.
Fourth channel detector can be easily added to simultaneously acquire images of
Fluorescence can also be separated
four colors.
using unmixing (only available on
Equipment Required: 4-channel detector
spectral scan unit).
Colocalization analysis Easily determine if labeled substances High-accuracy colocalization analysis
are present locally in the same New 60x oil-immersion objective offers image acquisition with exceptional
locations. positional accuracy coefficient.
Calculate of Pearson coefficients, Equipment Required: PLAPON 60xOSC
overlap coefficients and colocalization
indices.
* SIM scanner and TIRFM scanner cannot be installed on the same system.
** For more information about peripheral equipment, contact your Olympus dealer.
105
105
125
Pixels
Pixels
125
130
130
0
0.5
1
1.5
P1
P2
P3
P4
P5
21
Expandability
350350300300 400400 450450 500500 550550 600600 650650 700700 750750 800800
405 LD
440 LD
473 LD
6
35 LD
55
9 LD
458 M
ulti Argon
515
M
ulti Argon
543 G
reen
HeNe
488 Argon
mCherry
22
Fluorescence Illumination Unit
Stand with Mercury lamp house, motorized shutter,
and fiber delivery system for conventional fluorescence
observation. Light introduction via fiber optic port.
Transmitted Light Detection Unit
External transmitted light photomultiplier detector and
100 W Halogen conventional illumination, integrated
for both laser scanning and conventional transmitted
light Nomarski DIC observation. Motorized exchange
between transmitted light illumination and laser
detection. Simultaneous multi-channel confocal
fluorescence image and transmitted DIC acquisition
enabled.
Scanning Unit for IX81 Inverted Microscope
Dedicated mirror unit cassette is required.
Scanning Unit for BX61/BX61WI Upright Microscopes
Fluorescence illuminator integrated with scanning unit.
Laser Systems
The multi-combiner enables combinations
with all of the following diode lasers: 405
nm, 440 nm, 473 nm, 559 nm and 635 nm.
The system can also be equipped with
conventional Multi-line Ar laser and
HeNe(G) laser.
Single Type
Single channel laser output. AOTF is standard
equipment.
Scanning Units
Two types of scanning units, filter-based
and spectral detection, are provided. The
design is all-in-one, integrating the
scanning unit, tube lens and pupil
projection lens. Use of the microscope
fluorescence illuminator light path ensures
that expandability of the microscope itself
is not limited. Visible, UV and IR laser
introduction ports are provided, as well as
a feedback control system.
Illumination Units
Conventional illumination modules are
designed for long-duration time-lapse
experiments. Since light is introduced
through fiber delivery systems, no heat is
transferred to the microscope.
Optional Upgrade Equipments for FV1000
TIRFM Unit
Enables control of the necessary volume
of excitation light using FV1000 software. This unit enables TIRF imaging
using the laser light source used with
Confocal.
Fiber Port for Fluorescence Output
Confocal fluorescence emission can be
introduced via fiber delivery system into
external device. Fiber port equipped
with FC connector (fiber delivery system
not included).
23
4th Channel Detector Unit
Attaches to the optional port of either
the filter or spectral type scanning unit
and is used as a 4th confocal fluorescence detection channel. This is a
filter-based fluorescence detection unit.
SIM Scanner
Second scanner dedicated for laser light
stimulation, synchronized to the FV1000
main scanner for simultaneous laser
light stimulation and confocal image
acquisition. Independent fiber optic laser
introduction port. Dichromatic mirror
within motorized optical port of the scan
unit required for introduction of laser
into main scanner.
Dual Type
The multi-combiner outputs laser light with two fibers.
Light can be used both for observation and laser light
stimulation.
Expandability
FV1000 System Diagram
IX81-ZDC
Focal drift compensation for long timelapse imaging.
* Requires IX81 microscope. For information about ZDCcompatible objectives, contact your Olympus dealer.
CO2 Incubator/
MIU-IBC-IF-2, MIU-IBC-I-2
Highly precise incubator control keeps
the environment inside a laboratory dish
completely stable, at just below 37°C
temperature, 90% moisture and 5%
CO
2 concentration; in this way, live cell
activity can be maintained for
approximately two days.
* Not available in some areas
High-Precision Motorized Stage/
PRIOR H117
Multi-point time-lapse photography
using a 35 mm glass-bottom dish is
easy to perform with this motorized
stage, which can reproduce previouslyset positions with extreme precision. It
also allows efficient photographing of
multiple cells and detection of individual
cells showing expected reactions.
24
Fluorescence illumination unit
LD635 laser
635 nm
LD559 laser
559 nm
AOTF Laser combiner
(Single-fiber type)
HeNeG laser
543 nm
Select either laser
Multi Ar laser
458, 488, 515 nm
AOTF Laser combiner
(Dual-fiber type)
LD473 laser
473 nm
Select either laser
LD440 laser*
440 nm
LD405 laser*
405 nm
IR laser*
Transmitted light detection unit
2 incubator *
E
Scanning unit for IX81
(Spectral type or Filter type
detector system )
A
FG
CO
Motorized XY stage *
B
ABD
IX81
IX81-ZDC
Inverted motorized microscope
B
C
D
Scanning unit for BX61WI, BX61
(Spectral type or Filter type detector system)
F
G
DBEA
G
Cover *
TIRFM unit *
SIM Scanner*
C
Fiber port for
fluorescence
output*
4th channel
detector unit*
*Optional unit
E
BX61WI
BX61
Upright motorized microscope
FV Power supply *
F
Microscope
control unit
Software
Basic software
Review station software *
Diffusion Measurement Package *
FV power
supply unit
FV control unit
Multi Stimulation Software *
Multi Area Time Lapse Software *
Monitor
Objectives NA W.D. (mm) DIC prism Revolving
nosepiece
MPLN5X
0.10 20.00 –
WI-SSNP,
WI-SRE3
UMPLFLN10XW 0.30 3.50 WI-DIC10HR
WI-SSNP,
WI-SRE3
UMPLFLN20XW 0.50 3.50 WI-DIC20HR
WI-SSNP,
WI-SRE3
LUMPLFLN40XW 0.80 3.30 WI-DIC40HR
WI-SSNP,
WI-SRE3
LUMPLFLN60XW 1.00 2.00 WI-DIC60HR
WI-SSNP,
WI-SRE3
LUMFLN60XW 1.10 1.5 WI-DIC60HR
WI-SSNP,
WI-SRE3
XLUMPLFLN20XW 1.00 * 2.0 WI-DICXLU20HR WI-SNPXLU2
Objectives for fixed stage upright microscope
(using WI-UCD, WI-DICTHRA2)
* Note: These conditions are not met in confocal microscopy
W.D.
Cover glass
Correction
Condenser for BX2 Condenser for IX2
U-DICTS
Description NA thickness Immersion U-UCD8A-2 IX2-LWUCDA2
(mm)
(mm)
ring
optical element optical element
position
UPLSAPO4X 0.16 13 —
UPLSAPO10X2 0.40 3.1 0.17 U-DIC10 IX2-DIC10 normal
UPLSAPO20X 0.75 0.6 0.17 U-DIC20 IX2-DIC20 normal
UPLSAPO20XO 0.85 0.17 — Oil U-DIC20 IX2-DIC20 normal
UPLSAPO40X2 0.95 0.18 0.11-0.23 _ U-DIC40 IX2-DIC40 normal
UPLSAPO60XO 1.35 0.15 0.17 Oil U-DIC60 IX2-DIC60 BFP1
UPLSAPO60XW 1.20 0.28 0.13-0.21 Water _ U-DIC60 IX2-DIC60 normal
UPLSAPO100XO 1.40 0.12 0.17 Oil U-DIC100 IX2-DIC100 normal
PLAPON60XO 1.42 0.15 0.17 Oil U-DIC60 IX2-DIC60 BFP1
PLAPON60XOSC 1.40 0.12 0.17 Oil U-DIC60 IX2-DIC60 BFP1
UPLFLN40XO 1.30 0.2 0.17 Oil U-DIC40 IX2-DIC40 BFP1
APON60XOTIRF 1.49 0.1 0.13-0.19 Oil _ U-DIC60 IX2-DIC60 BFP1
UAPON100XOTIRF 1.49 0.1 0.13-0.19 Oil _ U-DIC100 IX2-DIC100 normal
UAPON150XOTIRF 1.45 0.08 0.13-0.19 Oil _ U-DIC100 IX2-DIC100 normal
Apo100XOHR 1.65 0.1 0.15 Oil U-DIC100 IX2-DIC100 normal
Objectives for BX2 and IX2
(using U-UCD8A-2, IX2-LWUCDA2 and U-DICTS)
Main Specifications
25
Spectral Version Filter Version
Laser Light Ultraviolet/Visible Light Laser LD lasers: 405 nm: 50 mW, 440 nm: 25 mW, 473 nm: 15 mW, 559 nm: 15 mW, 635 nm, 20 mW
Multi-line Ar laser (458 nm, 488 nm, 515 nm, Total 30 mW), HeNe(G) laser (543 nm, 1 mW)
AOTF Laser Combiner Visible light laser platform with implemented AOTF system, Ultra-fast intensity modulation with individual laser lines, additional shutter control
Continuously variable (0.1%–100%, 0.1% increment), REX: Capable of laser intensity adjustment and laser wavelength selection for each region
Fiber Broadband type (400 nm–650 nm)
Scanning and Scanner Module Standard 3 laser ports, VIS – UV – IR
Detection Excitation dichromatic mirror turret, 6 position (High performance DMs and 20/80 half mirror), Dual galvanometer mirror scanner (X, Y)
Motorized optical port for fluorescence illumination and optional module adaptation, Adaptation to microscope fluorescence condenser
Detector Module Standard 3 confocal Channels (3 photomultiplier detectors) Standard 3 confocal Channels (3 photomultiplier detectors)
Additional optional output port light path available for optional units Additional optional output port light path available for optional units
6 position beamsplitter turrets with CH1 and CH2 6 position beamsplitter turrets with CH1 and CH2
CH1 and CH2 equipped with independent grating and slit for fast and CH1 to CH3 each with 6 position barrier filter turret
flexible spectral detection (High performance filters)
Selectable wavelength bandwidth: 1–100 nm
Wavelength resolution: 2 nm
Wavelength switching speed: 100 nm/msec
CH3 with 6 position barrier filter turret
Filters High performance sputtered filters, dichromatic mirrors and barrier filters
Scanning Method 2 galvanometer scanning mirrors
Scanning Modes Scanning speed: 512 x 512 (1.1 sec., 1.6 sec., 2.7 sec., 3.3 sec., 3.9 sec., 5.9 sec., 11.3 sec., 27.4 sec., 54.0 sec.)
256 x 256 bidirectional scanning (0.064 sec., 0.129 sec.)
X,Y,T,Z,λ X,Y,T,Z
Line scanning: Straight line with free orientation, free line, Point scanning Line scanning: Straight line with free orientation, free line, Point scanning
Photo Detection Method 2 detection modes: Analog integration and hybrid photon counting
Pinhole Single motorized pinhole Single motorized pinhole
pinhole diameter ø50–300 µm (1 µm step) pinhole diameter ø50–800 µm (1 µm step)
Field Number (NA) 18
Optical Zoom 1x–50x in 0.1x increment
Z-drive Integrated motorized focus module of the microscope, minimum increment 0.01 µm or 10 nm
Transmitted Light Module with integrated external transmitted light photomultiplier detector and 100 W Halogen lamp, motorized switching, fiber adaptation to microscope
Detector unit frame
Microscope Motorized Microscope Inverted IX81, Upright BX61, Upright focusing nosepiece & fixed stage BX61WI
Fluorescence Illumination External fluorescence light source with motorized shutter, fiber adaptation to optical port of scan unit
Unit Motorized switching between LSM light path and fluorescence illumination
System Control PC PC-AT compatible, OS: Windows XP Professional (English version), Windows Vista (English version), Memory: 2.0 GB or larger, CPU:Core2Duo 3.0 GHz,
Hard disk: 500 GB or larger, Media: DVD Super Multi Drive, FV1000 Special I/F board (built-in PC), Graphic board: conformity with Open GL
Power Supply Unit Galvo control boards, scanning mirrors and gratings, Real time controller Galvo control boards, scanning mirrors
Display SXGA 1280X1024, dual 19 inch (or larger) monitors or WQUXGA 2560 x 1600, 29.8 inch monitor
Optional Unit SIM Scanner 2 galvanometer scanning mirrors, pupil projection lens, built-in laser shutter, 1 laser port, Fiber introduction of near UV diode laser or visible light laser,
Optional: 2nd AOTF laser combiner
TIRFM Unit Available laser: 405–633 nm. Motorized penetration ratio adjustment. Automatic optical setting for TIRFM objectives
4th CH Detector Module with photomultiplier detector, barrier filter turret, beamsplitter turret mounted with 3rd CH light path
Fiber Port for Fluorescence Output port equipped with FC fiber connector (compatible fiber core 100–125 µm)
Software
Image Acquisition Normal scan: 64 x 64, 128 x 128, 256 x 256, 320 x 320, 512 x 512, 640 x 640, 800 x 800, 1024 x 1024, 1600 x 1600, 2048 x 2048, 4096 x 4096
Clip rectangle scan ,Clip ellipse scan ,Polygon clip scan,line scan ,free line scan,Point scan, Real-time image
2-dimension: XY, XZ, XT and Xλ
3-dimension: XYZ, XYT, XYλ, XZT, XTλ and XZλ
4-dimension: XYZT, XZTλ and XYTλ
5−dimension: XYZTλ
Programmable Scan Controller Time Controller function
2D Image Display Each image display: Single-channel side-by-side, merge, cropping, live tiling, live tile, series (Z/T/λ),
LUT: individual color setting, pseudo-color, comment: graphic and text input
3D Visualization and Observation Interactive volume rendering: volume rendering display, projection display, animation displayed (save as OIF, AVI or MOV format)
Free orientation of cross section display
3D animation (maximum intensity projection method, SUM method)
3D and 2D sequential operation function
Image Format OIB/ OIF image format
8/ 16 bit gray scale/index color, 24/ 32/ 48 bit color,
JPEG/ BMP/ TIFF/ AVI/ MOV image functions
Olympus multi-tif format
Spectral Unmixing 2 Fluorescence spectral unmixing modes (normal and blind mode)
Image Processing Filter type: Sharpen, Average, DIC Sobel, Median, Shading, Laplacian
Calculations: inter-image, mathematical and logical, DIC background leveling
Image Analysis Fluorescence intensity, area and perimeter measurement, time-lapse measurement
Statistical Processing 2D data histogram display, colocalization
Optional Software Review station software, Off-line FLUOVIEW software for date analysis.
Motorized stage control software, Diffusion measurement package, Multi stimulation software, Multi area time-lapse software
Expandability
Dimensions (mm) Weight (kg) Power consumption
Microscope with scan unit BX61/BX61WI 320 (W) x 580 (D) x 565 (H) 41
—
IX81 350 (W) x 750 (D) x 640 (H) 51
Fluorescence illumination unit Lamp 180 (W) x 320 (D) x 235 (H) 6.7
Power supply 90 (W) x 270 (D) x 180 (H) 3.0 AC 100-240 V 50/60 Hz 1.6 A
Transmitted light detection unit 170 (W) x 330 (D) x 130 (H) 5.9 —
Microscope control unit 125 (W) x 332 (D) x 216 (H) 5.2 AC 100-120/220-240 V 50/60 Hz 3.5 A/1.5 A
FV Power supply unit 180 (W) x 328 (D) x 424 (H) 7.5 AC 100-120/220-240 V 50/60 Hz 4.0 A/2.0 A
FV control unit (PC) 180 (W) x 420 (D) x 360 (H) 10.5 AC 100/240 V 50/60 Hz 497.5 W
19 inch, dual (value per monitor) 363 (W) x 216 (D) x 389.5–489.5 (H) 5.9 AC100-120/200-240 V 50/60 Hz 0.65 A/0.4 A
29.8 inch 689 (W) x 254.7 (D) x 511.5–629.5(H) 15.7 AC100-120/200-240 V 50/60Hz 1.8 A/0.8 A
Power supply unit for laser combiner 210 (W) x 300(D) x 100 (H) 4.0 AC 100-120/200-240 V 50/60 Hz 2.0 A/1.0 A
Laser combiner (with Ar laser heads) 514 (W) x 504 (D) x 236 (H) 45 —
Laser combiner (without Ar laser heads) 514 (W) x 364 (D) x 236 (H) 40 —
LD559 laser power supply 200 (W) x 330 (D) x 52 (H) 1.2 AC 100-240 V 50/60 Hz 30 W
Multi Ar laser power supply 162 (W) x 287 (D) x 91 (H) 4.4 AC 100-240 V 50/60 Hz 20 A
HeNe(G) laser power supply 130 (W) x 224 (D) x 62 (H) 1.8 AC 100-120 V 50/60 Hz 0.45 A
Recommended FV1000 system setup
(IX81, BX61, BX61WI) (unit: mm)
Dimensions, Weight and Power Consumption
Display
*1 This product corresponds to regulated goods as stipulated in the "Foreign Exchange and Foreign Trade Control Law".
An export license from the Japanese government is required when exporting or leaving Japan with this product.
*2 The performance and safety of this device is not guaranteed if it is disassembled or modified.
*3 This device is designed for use in industrial environments for the EMC performance. (IEC61326-1 Class A device)
Using it in a residential environment may affect other equipment in the environment.
Hippocampal neurons
Courtesy of Dr. Shigeo Okabe
Department of Cellular Neurobiology, Graduate School of
Medicine, The University of Tokyo
Cultured nerve cells derived from the mouse hippocampus
Courtesy of Dr. Koji Ikegami, Dr. Mitsutoshi Setou
Molecular Geriatric Medicine, Mitsubishi Kagaku Institute of Life
Sciences
Cerebellum Purkinje cell
Courtesy of Dr. Tetsuro Kashiwabara, Assistant Professor; and
Dr. Akira Mizoguchi, Professor;
Neuroregenerative medicine course, Mie University School of
Medicine
Drosophila, Stage 14
Courtesy of Dr. Tetsuya Kojima
Laboratory of Innovational Biology, Department of Integrated
Biosciences Graduate School of Frontier Sciences, University
of Tokyo
"Brainbow" mouse brain stem
Courtesy of the laboratories of Jeff W. Lichtman and Joshua R.
Sanes Harvard University MCB Department and the Center for
Brain Science
Mouse brain section
Courtesy of Mr. Masayuki Sekiguchi (Section Chief)
Department of Degenerative Neurological Diseases,
National Institute of Neuroscience, National Center of
Neurology and Psychiatry
Rudimentary limbs of larva in latter part of 3rd instar
Courtesy of Dr. Tetsuya Kojima
Laboratory of Innovational Biology, Department of Integrated
Biosciences, Graduate School of Frontier Sciences, University
of Tokyo
Zebrafish
Courtesy of Dr. Toru Murakami,
Department of Neuromuscular & Developmental Anatomy,
Gunma University Graduate School of Medicine
Medaka embryogenesis (somite stage)
Courtesy of Minoru Tanaka, Hiromi Kurokawa
National Institute for Basic Biology Laboratory of Molecular
Genetics for Reproduction
Pilidium larva of Micrura alaskensis
Courtesy of Dr. Svetlana Maslakova of the University of
Washington and Dr. Mikhail V Matz of the Whitney Laboratory
for Marine Bioscience, University of Florida.
Osteoclast induced from rat monocyte in rat kidney
Courtesy of Dr. Keiko Suzuki,
Department of Pharmacology, Showa University School of
Dentistry
Fucci–Sliced mouse brain, expressing S/G2/M phases
Courtesy of Dr. Hiroshi Kurokawa, Ms. Asako Sakaue-Sawano
and Dr. Atsushi Miyawaki
RIKEN Brain Science Institute Laboratory for Cell Function
Dynamics
Immunolabeling of a transgenic mouse retina showing the
major retinal cells types
Courtesy of Dr. Rachel Wong, Mr. Josh Morgan
Dept. Biological Structure, University of Washington, Seattle.
Wild-type embryo in stage 17 of drosophila
Courtesy of Dr. Tetsuya Kojima
Laboratory of Innovational Biology, Department of Integrated
Biosciences
Graduate School of Frontier Sciences, University of Tokyo
Alpha Blend method (Cultured nerve cells derived from the
mouse hippocampus)
Courtesy of Dr. Koji Ikegami, Dr. Mitsutoshi Setou
Molecular Geriatric Medicine, Mitsubishi Kagaku Institute of Life
Sciences
26
Images are courtesy of the following institutions:
1310
680
1880
1200
Depth: 990
FLUOVIEW website
www.olympusfluoview.com
• OLYMPUS CORPOARATION is ISO9001/ISO14001 certified.
• Illumination devices for microscope have suggested lifetimes.
Periodic inspections are required. Please visit our web site for details.
• Windows is a registered trademark of Microsoft Corporation in the United States and other
countries. All other company and product names are registered trademarks and/or trademarks of
their respective owners.
• Images on the PC monitors are simulated.
• Specifications and appearances are subject to change without any notice or obligation on the
part of the manufacturer.
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