Olympus FV1000 User Manual [ru]
User’s Manual
[Hardware Manual]
FLUOVIEW
FV1000
CONFOCAL LASER SCANNING
BIOLOGICAL MICROSCOPE
FV10-ASW [Ver.3.0]
Notice
Thank you for your purchase of Olympus microscope at this time.
Retain this manual in an easily accessible place near a system for future reference.
AX8061
CAUTION
CAUTION
FV1000 is a CLASS 3B laser product.
The procedures for using this system are classified as follows:
z Service
“Service” means any adjustment or repair performed by highly trained and skilled technical personnels who
are provided the service training following to the service manual for this system.
The performance has influence on the feature of this system, and there is a risk which unintended CLASS
3B laser light is emitted.
z Maintenance
“Maintenance” means adjustment or other procedures performed by customers to maintain that this system
functions properly.
z Operation
“Operation” means all performance described in the user’s manuals in this system.
CLASS 3B laser light is only emitted from the objective lens during the actual execution.
The User’s Manuals of this system consist of the following:
In order to maintain the full performance of this system and ensure your safety, be sure to read these user’s
manuals and the operating instructions for the laser unit and light source unit before use.
z FV1000MPE / FV1000 User’s Manual [Laser Safety Guide]
z FV1000 User’s Manual [Safety Guide]
z FV1000 User’s Manual [Hardware Manual]
z FV1000 FV10-ASW User’s Manual [Quick Start]
Also, we have prepared one service manual for this system as below. Technical personnels who perform the
service require to take the service training.
z FV1000MPE / FV1000 Service Manual
Reproduction, copying or duplication of a part or all of this software and manual is prohibited.
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Registered Trademarks
Registered Trademarks
Microsoft, Microsoft Windows, Excel for Windows are registered trademarks of Microsoft Corporation.
Other brand names and product names are trademarks or registered trademarks of their respective owners.
MANUAL CONFIGURATION
I. SYSTEM OVERVIEW
1. System Overview............................................................................................I. 1-1
II. PREPARATION For OPERATION
1. Preparation for Operation...............................................................................II. 1 - 1
2. Replacement of Cubes...................................................................................II. 2 - 1
3. Centration of Mercury Burner .........................................................................II. 3-1
4. Replacement of Lamps ..................................................................................II. 4 - 1
III. TROUBLE Q&A
1. Troubleshooting Guide ...................................................................................III. 1-1
NOTATIONS IN THIS MANUAL
This manual complies with the following notations.
Notation of Caution, Notes and Tips
Notation D e s c r i p t i o n
It indicates caution to prevent physical damage to user or
damage to product (including furniture in peripheral area).
2
NOTE
TIP
Indicates details of caution to prevent damage or deterioration
of products.
Hint or one point advice appears with
TIP
attached.
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This volume describes the overview of the FLUOVIEW FV1000
system.
Please read this volume so that you can understand the
system before use.
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CONTENTS
1 System Overview 1-1
1-1 Principles.......................................................................................... 1-1
1-2 Features of the FV1000 ...................................................................1-2
1-3 Optical Path Diagram ......................................................................1-3
1-4 System Configuration .....................................................................1-4
1-4-1 System Diagram............................................................................................ 1-4
1-4-2 System Appearance and Functions............................................................... 1-5
System Overview / Principles
I.
1 System Overview
OLYMPUS FV1000 is a confocal laser scanning biological microscope system featuring improved basic
performances (sensor system, scanning system and illumination system performances) by considering the “live
cell observations”, with which long hours of stable measurement of weak fluorescence is required.
This microscope is equipped with 3 fluorescence channels, 3 lasers and AOTF to meet various applications in
a wide range of advanced research fields.
1-1 Principles
A laser scanning microscope converges the laser beam into a small spot using an
objective and scans the specimen in the X-Y direction using the laser beam.
The microscope then captures the fluorescent light and reflected light from the
specimen using light detectors and outputs the specimen image on an image monitor.
As shown in this figure, the confocal
optics incorporates a confocal aperture
on the optically conjugate position
(confocal plane) with the focus position
to eliminate light from other part than the
focus position. This causes the
extraneous light to be viewed as
darkness in the observation image, it is
possible to slice optically a tissue
specimen that has thickness.
On the other hand, an ordinary optical
microscope, the light from other part
than the focus position is overlapped
with the imaging light of the focus
position so the image is blurred in
overall.
The laser beam that has transmitted
through the specimen is detected by the
transmitted light detector and provides
the transmitted image, which is not a
confocal image.
However, when the fluorescence images of the transmitted and confocal images are
combined, it is possible to obtain very important information on the specimen.
Light detector
Confocal
aperture
Laser
Objective
Specimen
Light detector
Page
I.
System Overview
1-1
System Overview / Features of the FV1000
I.
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1-2 Features of the FV1000
1. The photon counting mode is newly provided to improve the sensitivity and S/N
and to enable quantitative optical intensity measurement. Photon counting makes
possible long hours of quantitative observation by completely eliminating
analog-derived drift. The dynamic range in which photon counting is possible is
expanded using a newly designed wideband head amplifier and processing
circuitry.
2. High-speed imaging at 8 frames per sec. is made possible by fast galvano mirror.
In addition, high-speed image acquisition is possible without stopping the Z-series
motors used in the XYZ and XZ observations.
3. During long hours of time-lapse observation, a stable supply of excitation light is
made possible thanks to the feedback control of the intensity of each laser.
Together with the photon counting function, this function ensures the stability and
quantitative nature of long-hour observations.
4. Three fluorescence channels, three lasers and AOTF are provided as standard to
meet a large variety of applications.
5. With a fully-motorized scan unit and motorized microscope, the entire system is
motorized so the scanning conditions including those of the optics can be saved
and reproduced.
6. When an extension laser irradiation unit is used for photon activation aiming at
causing discoloration, optical simulation or uncaging of the specimen, a system
optimized for cell function analysis experiments can be built.
7. When the system incorporates the spectral detector unit that is composed of a
2-channel spectral detector and 1-channel filter, it is possible to set the detection
conditions more flexibly, acquire the fluorescence spectral data and use the
fluorescence isolation function.
I.
System Overview
1-2
Page
I.
1-3 Optical Path Diagram
System Overview / Optical Path Diagram
Laser beam
Galvano mirror
VIS
Ch3
UV
Ch4
Barrier filter
IR
Barrier filter
Grating
Ch2
DM
Slit
Ch1
DM
DM
Pinhole
Grating
Slit
Mercury light Supply
Galvano mirror
DM
Page
I.
System Overview
1-3
I.
FV5-LA-MAR2
LD405/440 Laser
Laser Combiner
FV5-LA-HEG2
dvanced
Advanced software
Review Software
PC Interface Board
VIS laser Fiber Unit
Mercury lamp Housing
Epi Fiber
Illumination Unit
Power Supply Unit
Additional 4th Channel
Fluorescent Sensor
Spectral
Fluorescent
Fiber Port for Fluorescent Detection
Scan Unit
for BX
Scan Unit
for BXWI
Filter Set
for SPD MBGR
Detector
System Controller
Filter Set
for FD MBGR
Filter Type Fluorescent
Detector
20’ Flat Panel Display
Control Box for
Microscope
Mercury Lamp Housing
Transmitted Light Detector
Epi Fiber
Illumination Unit
Non-Confocal
Detector for IX
System Overview / System Configuration
Non-confocal Point
Detector for BX
Microscope
Swinging Nosepiece
1-4 System Configuration
1-4-1 System Diagram
XLU Single-Position Nosepiece
Anti-
vibration Table
Scan Unit for IX
Power Supply Unit
Additional Scan Unit
Non-Confocal Detector for SU
Microscope
Air
Anti-Vibration
Table
System Overview
I.
Page
1-4
I.
1-4-2 System Appearance and Functions
r
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The applicable microscopes are the BX61/62TRF, BX61WIF and IX81F.
Epi Fibe
Illumination Unit
㪝㪠㪩
Microscope
Top View
System Overview / System Configuration
Laser Power Supply
Mouse
Keyboard
Epi Fiber Illumination Unit
Illumination unit based on
mercury burner, connected to
the microscope through a fiber.
Scan Unit
Heart of lase
scanning
microscope, composed
of scanner and light detector.
Microscope
Designed for fluorescence
observations.
Anti-vibration table
Control box
Controls
the microscope.
Mercury power suppl
Power supply for reflected
light illumination.
Microscope
UCB
RFL
VT
Power supply unit
lso controls the FV1000 scan
unit, laser combiner, etc.
20’ Flat Panel Display
Monitors for displaying
the laser scanning image
and control panel, etc.
LCD LCD
PSU
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TD
Transmitted light detector
(incl. transmitted light illumination unit)
Unit for obtaining the transmitted
image, connected to the
microscope through a fiber.
Power suppl
Power suppl
Keyboard
MCOMB/S
MCOMB/D
Laser power supply
Power supply for Multiline Argon, HeNe
green and LD559 lasers.
System Controller
Used to control
the FV1000, file
its images, etc.
PC
Power supply
Power supply
for Multi
laser combiner.
Laser combiner
Unit for combining the LD
laser, the gas laser, and so on
into a single fiber.
Front View
I.
System Overview
Page
1-5
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