Olympus FH Instructions Manual

olympus

microscope

scanned

by

J.

G.

McHone

• t .

21

Nov

09

NOT

FOR

SALE

zns

ructzons

Olympus

-Tokyo

scannedbyJ.

G.

McHone 21

Nov

2009

forpersonal use

only,

not

for sale

OLYMPUS MICROSCOPE MODEL

FH

The new Olympus

FH

Microscope was made

in

response

to

a wide

demand

for

a microscope

with

perfect

illuminator.

This Microscope, then,

is an improvement on the

highly

popular F Microscope, whose

fine

adjustm-

entis0.0005

mm, and whose heads, stages, and lenses are interchangeable.

The Olympus

FH

Microscope, therefore, is a microscope we can recom-

mend

to

the public

with

pride and confidence. The Olympus Optical Co.,

ltd,.

we

might

add, was established in 1919, and since has been

turning

out

optical

products exclusively.

OUTSTANDING FEATURES

OF

OLYMPUS

FH

MICROSCOPE

1.

Perfect Built·in Illuminator.

Centering need only

be

done once regardless of changes in magnification.

2.

0.0005

mm

Fine Adjustment.

This microscope boasts

an

ultra-sensitive fine adjustment system, which

is

the first of its kind in the world.

Focusing is

easy,

and, what

is

more, the thickness

of

the specimen

can

be

measured

by

the sensitivity scale.

3.

Interchangeability of

Head

and

Stage.

This microscope is available in

any

desired head·stage combination.

Furthermore, the

head

and stage

can

be

changed easily

and

speedily.

4.

Plan

Achromatic Objectives.

The

plan achromatic objectives

haye

been

so

designed that there is no

spherical aberration. Furthermore, the field of photomicrographic work

can

be

widened

by

using

low

magnification

plan

aChromafic objectives.

STANDARD COMBINATIONS

OF

FH MICROSCOPE

1.

Binocular

Body

Type

Eyepiece

Stage

Condenser Magnifications

FHF - Bi - I P7X, WFlOX

FS

N.A.I.25

28X·1500X

PI5X

(paired)

FHF - Bi -

II

P7X, WFIOX

FS

N.A.1.25

28X -

2000X

PI5X,

K20X

(Paired)

FHF- Bi

-III

FS

N.

A.I.25

28X -

2000X

2.

Trinocular

Body

Typa Eyepiece

Stage

Condenser

Magnifications

FHF·Tr- I P7X. WFlOX

FS

N.A.I.25

28X

·1500X

PI5X

(Paired)
P.7X,

P.lOX

P.

15X

(one

each

for

photomicro.)

FHF -Tr-

II

P7X, WFIOX FS N.A.1.25

28X -

2000X

PI5X,

K20X

(paired)

P.7X,

P.IOX

P.

15X

(one eac

10<

photomicro.)

FHF -

Tr-III

PJan4X

FS

N.A.1.25

28X

-2000X

P1anll'l

Plan40X

P.7,f':--.IOX

Plan

;(lOX

(oj

mmersion)

P.

15X

(one each

for

photomicro.)

2

NAME

OF

PARTS

Arm

...,

Condenser Centering Screw

---~......J

Coarse Adjustment Handle

----_

Fine Adjustment Handle

-----

Coarse

Adjustment

Stopper

------

Lamp Centering Screw

Socket

Clamp Screw

SPECIFICATIONS

'r----------

Eyepiece

,--------

Prism Housing

...-....

,.----

Revo!vinS NosepIece

~----

Objective

Stage

r------

Iris Diaphragm Lever

"------

Filter Holder

'-------

Stage

Stopper

Condenser

Vertlca

I

.----------

Movement

Handle

Projection Lens

Sase

Field

Diaph"~gm

Body

Tube:

Revoll/jng

Nosepiece:

BUlb:

Transformer:
Filter:

Ught Source:

Focusing;

Coarse

Adjustment:

Binocular Heed

-,

45ftInclination, rotatable through

3600horizontally.

Trinocular Head Binocular

part

45ftinclination, rotatable through

360"

horizontally.

Quadruple (ball bearing

system)

indicator for obJectives' position.

6V 5A

TB-l

(clear)

Te.

IOOV.

IIOV.

220V.

240V

aYallable.

32.5~mm

(blue)

Bulb centerIng

adjustment

type;

compensatIon lens Shifting system

(for

low, medium and high

light

value)_

Vertical

movement

stage; co-axial coarse and fine

adjustment

handles..

Rack-tlnd-pinion with

stopper;

range of

operation;

32.5mm

3

OPTICAL PATH

~--

<-----~

----

----

LL_~

__

/

Fine

Adjustment:

Balanced

spring-tension

system;

rangeofvertical

movem@nt:

1.2mm;

scale;

calibratedInunitofO.OOlmm.

Condenser

Focusing

System:

Rack-aod-pinion

ty~e;

range

of

vertical

movement:

23.5mm;

centering

device.

Condenser:

N.A.

1.25

condenser

with

external

diameter

of

36.8mm

and

featuring

varlabre

iris-diaphragm

and

filter

holder.

Stage:

SCluare Coaxial Mechanical

Stage

(double-handle)

F'S.-....

easy

to

detach

and replace

the

stages,

asit15

dovetailedtothe

body.

Special

Attachments:

The Round Revolving

Stage

Model

RS.

The SCluare Coaxial

Mechanical

Stage

(single-handle)

Mod@1

es.

The

Square

ReYolvlng Coaxial Mechanical

stage

(single-handle)

model

erS,

4

STRUCTURE

OF

PARTS

A.

MICROSCOPE

BODY

Arm,

Focusing Mechanism and Condenser:

The

armissecured

firmly

to

the

basebymeansofa

support,

and.inturn,itsupports

such

partsasthe

focusing

mechanism,

stage.

condenser,

revolving

nosepiece

and

the

head.

furthermore.

It

is shaped

uo

facitrtate

carrying

around.

The

focusing

mechanism

has such

builtinunrts35the

coarse

adjustment

and

fine

adjustment

units

and

the

coarse

adjustment

stopper.

The coarse

adjustment

handle

and

the

fine

adjustment

handle

are

coaxial.Inother

words.

the

large

handleisused

for

coarse

adjustment

and

the

small

handle

for

fine

adjustment.

The

stage

will

movenOhIgher

than

the

height

obtained

by

manipul

ating

the

coarse

adjustment

handle,

provided

that

the

coarse

adjustment

stopper

is

tightened.

In

othe

words.

when

the

stageislowered

and

again

brought

up

toward

the

objecti\le.

it

will

go

no

higher

than

the

stop

position.

This

not

onl)l

pre~ents

contactofthe

specimen

and

the

objective

but

also

facilitate5

focusins.

The

condenser

mount.

which i5ofthe

rack-and-plnlon

type,

has

it

recei~er

withadovetail

groove. ThB

condenser

mount

l:;crewisused

for

adjusting

the

optIcal

axis.

~

Adjustment

Handle-

....

+.,..~!LJ

I

Adjustment

Handle

----Condenser

Fitting

Screw

'----

Iri5 Diaphr.8gm Lever

'----

Filte, Holde,

"------

Condenser

Centering

Screw

'-------

Stege

Stopper

Coarse

Adjustment

Stopper

5

B.

MICROSCOPE

HEAD

The

mitroscope

headofModel

EH

microscopeisinclined

45

degrees

from

the

horizontal

plane

so astoenable

microscopic

examinationinthe

most

natural

posture

and

with

is

minimum

of

fatigue.

As

mentioned

previously,

the

standard

heightofthis

instrumentIs380mm

from

the

desktop.

Thisisex.actly

the

height

required

for

the

average

persontoconduct

microscopic

examination

In

the

most

natural

sitting

posture,

Thus, a special

deskisabSOlutely unneceSSary when

this

MOdel

EH

microscope is used.

The

following

four

typesofmicroscope

h~ad

are

available;

1.

Binocular

head

(Bi)

2. Trinocular

head

(Tr)

3.

Upright

head

for

photomicrography

4.

Monocular

head

To

mount

the

mu;:roscope head,

turn

the

knurled

rinBofthe

microscope

fix.ing

screw.

This

witt

fix

the

head

properly

to

the

arm

and;

at

the

sam~

time,

secure

precise

optical

align-

ment.

Microsc.ope

h@:ad

fixing

guide

3600Ranso

of

adjustment

of

direction

01

micro

scope head

B·1

Binocular Head

I

Microscope

head

filcing

ring

Ttle above he.ads,

with

the

exception

of

the

upright

head

for

photomicrography,

which

remains

In a

fix@d

position,

can be

rotated

through

360

0

horizontally,

The

light

from

the

objective

reach@$

the eyepieces

after

being

split

into

two

by

the

semi.

transmissIve

prism.

This

head was

designed

taking

Into

consideration

the

Characteristic

function

of

the

human

eyes_

Thatisto

say. the

right

and

left

optical

axis

are

slanted

slightly

toward

the

center.

Furthermore,

the

eyepieces can be

adjusted

according

to

the

user's

inter-pupillarv

distance.

Thisisdone

by

sHpping

out

the

dovetailed

eyepieces. The

inter-pupillary

distanceIsshownOnthe

scale

between

the

two

eyepieces. This

operationiscarr@d

eye-width

adjustment.

The scale readinBs are

based

on BiWFlOX eyepie:ces. Since one

e~e

is

often

weaker

than

the

other,

this

tubeisequipped

withadiopter

adjustment

ringonthe

left

eyepiece. When

the

dioptric

adjustment

ring

is

setat2.ero,

and

the

inter-pupillary

distance

scale

reads

62,

the

mechanical

tube

measures

aDoul

160mm

long.

6

B·2

Trinocular

Body

The

trinocular

head

consistsofbinocular

head and

upright

head

for

photomic.rography.

The

photo,trapller

can actually see

what

he

is

photographing

since

it

inCO(PO~iJtes

a

mechanism

to

switch

the light

pateh

alternately

for

direct

observation

and

photomicrographic

work.

The

binocular

head is

equipped

withiJdioptric

adjustment

ring

andanInter-pupHlary

distance

scale,

Straight

tube

for

photomicrography

prism

shiWng

knob

When the

prism

shlftlng

knob

is pUlled

outward

as

shown

in the above

photo.

the

prism

locks

into

position

(0)

diagram)

and

the

pathofraysisdirec-

tedtolhe

upright

head

for photomicrographic work.

A

PUSh

in

of

the

knob

almost

instantly

returnS

the

prism

to

its

original

position

(@

diagram).

ThIs unique

mechanism

(one-knob

operation)

permits

direct

observation

and

photomicrographic

work

alternately

with

a minimum loss

of

time.

rn

Pathofraysatthe

time

W

of

photomicrography

00

Path

of

rays

at

the

time

of

direct

observation

Inter-pupillary

distance

scale

Dioptric

adjustment

ring

set

at

zero

B-3

Upright

head

for

photomicrography

In caseofmicroscopes

featuring

monocular

inclined headorbinocular

head,

the

standard

microscope

head

must

be

interchanged

with

an

upright

head

when

conducting

photomicro·

graphic

work.

The

photomicrographic

appara·

tus

must

be

mounted

on

this

upright

head.

B-4 Monocular Head

7

C.

STAGE

There

Ire

four

typesofstages

available

for-

Model

F'H:

o Square coaxial

mechanical

stage

(double

handle)

FS:

o Square

revolving

coaxial mechanical

stage

(single

handle)

erS

o Square coaxial

mechanical

stage

(single

handle)

CS

o Round

revolving

stage

RS

The square

cOClxial

mechanical

stage

fSisstandard

equipmentofthe

fH

Microscope.

C·l

Square Coaxial Mechanical

Stage

FS

ThIs square

stage

can be detachedatwill sinceitis

dovetailedtothe

main

body.

The

specimen

can be moved

by

meansofhandles

found

on

both

sidesofthe

stage.

For

forward

and

backward

movementofthe specimen, a

large

handle

(raCk-aod-pinion)

is

used,

andasmall

handle

(lead

screw)

is used

for

lateral

movement

Th{J

surfaceofthe

stage

can

be made levelbYremoving

the

Clip.
Two

slide glasses

canbeplacedonthe

specimens

side

by side.

Thisisextremely

convenient

when

comparing

two

specimensatthe

same Ume.

Lateral

movement

handle

Lateral

movement

scale

----'l---=

forward

and

backward

movement

handle

Forward

and

backward

movement

scale

Lateral

movement

handle vernier

Forward

and

backward

movement

handle

8

P.

lOX

Binocular

head

P 15X

Eyepieces

Photo

lenses

~

Monocular

inClined head

MODEL

FH

VARIED COMBINATION

OF

PARTS

Ob)ecti\lu

Round

stage

RS

Abbe

condensers

N A 1.25

-

P.

7X

Trlnocular

head

Ugright head

Square

mechanical

stage

(single handle)

CS

N.A.I.40

Square

revolving

mechanical

stage

(single handle)

C,S

SWing-outN.A.

0.25-0.9

Square mechBnical stage

(double handle)

FS

C-2

Square Revolving Coaxial Mechanical Stage (single

handle)

CrS

This

stageisfixed

firmly

to

the

bOdYofthe

microscope.

and on

Its

surfaee,

there

is a

cross

movement

clip. The

specimen

is

shifted

by

means

0"acoa;a:iar

type

forward

and

backward

movement

handle and

lateral

movement

handle on

the

right

sideofthe

stage. The

tormer

Is

operated

by

meansofa rac.k-and-pinion. whereas

the

latterIsmanIpulated

by

means

of

a lead

screw.

The

workIng

ranseofthe

handles

are:

Forward

and

backward

27mm

Lateral

_ _

70mm

Each

handle

has a scale

(0-30.

40-110)

and a

vernier.

Rotation;

After

st!'tting

the

slide glass in

position,

the

centerofthe

slide

glassisbrought

Into

line

with

the

optical

axis.

Then

the

sta86

j!i

rotated

horizontally.

The stage

rotates

about

220·

to

clockwise,

and

about

200:.tocounter-cloc:kwise.

Rotation

can be

stoppedbymeans

of

the

stage

stopper,

Rotating

220·

Lateral

movement

handle

~

Forward

and

backward

movement

handle

C-3

Square Coaxial Mechanical Stage (single handle)

CS

This

mechanical

stage can be

operated

byasingle

handle

which

consistsofa

rack-aod-pinion

for

forward

and

backward

movement.

and a lead

SCrew

for

lateral

movement

Lateral

movement

scale

Forward

and

backward

movement

handle

Forward

and

backward

movement

scale

lateral

movement

t1andle

11

C-4

Round Revolving Stage

RS

The

round

revolving

stage

consistsoftwo

stages.

The

lower

stage

is

fixed

to

the

stage

mount

On

the

main

body.

The

upper

stage slides over tne

lower

stage

horizontally.

Sliding Is donebyoperating

the

left-

and

right-

side

fine

adjustment

handles

by

turns.

Furthermore,

the

upper

stage

can be

rotated

through

360"

horizontally

by

pressing

the

knurl

part

on the

rimofthe

stage.

Clip

Stage fine movement handle

How

to

Fix Stage

to

the

Body

The Olympus FH

stases

have beensomade

that

they

can be

easily

fixedtothe

main bodY.

The

four

stagos rs. CrS,

CS

and

RS.

eaen

of

which is used

fer

a specifiC purpose _ are

Jnterchangeable.

1.

Firstofall,

the

condenserisloweredasfaraspossiblebymeansofthe

condenser

handle.

2.

The

stage

mountisalso

lowered

l!I$

faraspossiblebyturning

the

coarse

adjustment

handle,

3.

In

the

caseofFS. CrS

and

CS

stages,

the

clip

is

moved

forward

as

far

as

possible

by

manipulating

the

forward

and

backward

maven

lent

handre.

4.

Next,

the

stage

guide

(,A)

is

slowly

inserted

Into

that

of

the

main

body

(8)

with

the

lock-rever

up.

The stage

must

be so

Inserted

thatitis

parallel

with

the

main

bodY, Care

must

be

taken

not

to

crash

the

top

lens

of

the

condenser

with

the

stage

during

this

operation.

5.

Finally,

the

stage

firmly

fixedtothe

main

bodybymeansofthe

rock-lever.

(A)

lock-lever

12

(B)

D.

REVOLVING NOSEPIECE

The revolvlllB

nosepieceofModel

FH

featuresaball-alld-socket

revolving

system

with

four

objective

mounts.

A

knurled

rillS is

provided

for

slip-free

and

smooth

manipulation

of

the

nosepIece.

By

meansofthe

large

built-in

spril'lg

and

groove,

the

objll:!e:.tive

s.ets

into

Position

ac.curately.

maintaining

proper

optical

alignment.

ihe

upper

'Surfaceofthe

(evolverismarked

with

the

letters

A,5,C,

D in

ordertoShow where tne

objectives

s,hould be

fixed.

'A'i5far

4X,

'8'

fOr

lOX.

'C'

for

40X,

and

'0'

for

lOOX. In

this

way,

the

position

of

the

objectives

do

not

change.

Furthermore,

the

observe..can

tell

which

objective

is

being

u5ed while

conducting

observation.

B

(lOX)

A

(4X)

E.

BASE AND ILLUMINATOR

Letter

specifying

position

of

differ8nt

objective

Tile

base

not

only

supports

the

microscope

but

also has

built-in

illuminator.

The

light

source

unitisfixedtothe

basebymeansoffitting

screws.

Switch

over

the

Or'st

compensation.

tens

according

to

the

mCli811ification

of

the

objective

being

used.

The

compensation

lens

shiftll1g

lever

Is used

for

the

following

purposes.

l

(Low)

objective

4X

or

lass

M

(Medium)

objective

lOX

and

20X

H

(High)

objective

40XormOre

Socket

Clamp 5cr'ew

lamp

Centering

Screw

Field

Stop

13

Projection'

Lens

lens

(Low)

L-

__

M

(Medium)

(4X

or

'.ss)

6V.5A

\ I

~

.~----J

f '

1-:--<.

i

I·_·-J

I

~

ICOlYJ

I~

-/

')

Lr--j-<-----------'L

---J

Field

stop

For

low

magnification

For

high

magnification

(40XormOl'"e)

EFFECTIVE ILLUMINATION

PriOrtoconducting

microscopy,itisofvery

importance

to

check

whether

appropriate

and

effecth"e

lightinSisdirectedtothe

specimen.

Thatisto

say,

the

observer

should seeifthe

ray

of

light

emitted

from

the

illuminatorOrmirror

followsacentral

path

leadinstothe

obiective

and

eyepieces.

This

can be donebylook

ins

into

the- eyepiece tube

after

roughly

focusing

a 40X

objective

or

lOX.

objective

and remo\lling

the

eyepiece.

If

the

light

from

the

light

source

unit

illuminates

throughout

the lens surfac.e evenly without: any

eccentricity,

it

means

that

proper.

effective

lightingisbeins rendered.

E\lIenifthe

lightisnot

eccentric., only

the

centerofthe

lens

is

often

illuminated

brightly.

This

means

that

the

condenserisnotinthe

proper

position.

This

canbecorrectedbyadiusting

the

positionofthe

condenserbymovingitup and

down.

However.

it

should be

notea

that

inaccurate

focusing

produces

the

same

phenomenon.

Even

the

slightest

mistake

can

throw

the

optic

path

off

center.

To

illustrate,

the

observer

should

not

even

touch

the

mirror

sinceitresults

ifl

poor

illumination.

Furthermol'e,

wnen

the

image appearStomove

off

the

center

while

manipulating

the

fine

adjustmeflt

handle in

the

COurse

of

securing

focus.itmeans

that

the

pathofraysisnot

aligned propel'ly. In

this

case,

lighting

must

be

readjusted

in accordance

with

the

fore,going procedures.

CENTERING

OF

LIGHT

Centel'"ingoflight

from

the

light

SOUl'eeisessentialtoordinary

micl'oscopy

and

mlcropl'loto·

sraphy,

The

followl"g

procedul'"es

permit

to

Obtain soo(J

centeringofligl1t.

1. Focus

the

specimen

with

lOX

objective.

2. Close

both

Il'i$ diaphragmoftne

condenser and field

stopofthe

illuminator,

Bring

~Ield

stop

(8

small

circleofstop-lisht)

into

focus

by

moving

the

condenser

up and down.

14

3. Set field

stop

(stop-light)

at

the

centerofthe field by

manIpulating

two

condenser

centering

screws.

4.

Open field

stop

slowly so

that

an

entire

field

can

be

seen.

5.

Remoye

the

eyepiece

from

the

e}'epiece

tube,

and

open

iris

diaphragm

slowlY, While looking

into

the eyepiece

tube,sothat

an

entire

field

can be

lighted.

6.

By

adjusting

the

lamp

centering

screws

(2

screws)

of

the

lamp

house,

set

the

image

of

filamentatthe

centerofthe

field.

7. Replace the eyepiece. and

start

your

microscopic

examination.

NOTES:

(1)

When

low

magnification

objective.

particularly

4X

objective,

Is used,

the

field

is

often

illuminated

unevenly. In

this

case,

adjust

lightingbyoneofthe

following

manners.

(s)

Loosen

the

socket

clamp

sCrew and move

the

socket

back

and

forth

slowly, while

looking

into

the eyepiece tube, and

fbe

the

socketata

position

where an even

light

can be obtained.

(b)

Remove

the

condenser and replaceItafter

takinB

off

the

top

tens. In

other

words.

use

the

condenser

without

the

top

lens.

(c)

Put

ground

glassonthe

projection

lensofthe

illuminator.

(2)

Condenser.
The

numerical

apertureofcondenser is

1,25

when

011

is used

for

oil

immersion

microscopy.

otherwiseitis about N.

A.I.

0

......

0.9,

and N.

A.

0.5

without

the

top

lens.

PROCEDURE

FOR

ADJUSTING THE COARSE ADJUSTMENT HANDLE

Generally speaking,

the

coarse

adjustment

nandles

are

tight.

This.ofcourse,

means

that

movement

is heavy.

However.

this

can be remedied easily by

gripping

the

right-and

left-side

handles as

illustrated

and

turning

theminthe

opposite

direction

SImultaneously. In

most

cases,

the

coarse

adjustment

handles

should be

moreorless

tight

rather

than loose.

WORKING DISTANCE AND PAR·FOCAL

The revolving nosepiecetowhich

the

objectives

are

attached,

has been

so

designed

that

as

long as one

objective

has been

focused

properly

the

others

can

be

focusedbymeansofthe

fine

adiustment

handle. The

distance

from

the

tipoflow

magnificiltion

objectlvetothe

specimen

is

considerably

100l3er

than

that

from

high

magnification

objective.

The

distanceisshortestinthe

caseofoil

Immersion

objective,

When

the

reVOlving nosepiece is

rotated,

after

focus

is secured

with

one

of

the

objectives.toSWitch

the

magnification

from

onetothe

other,

1nr Instance,

to

the

oil

immersion

objective.

thereisno

dangerofthe

tipofthe

lens

touching

the

specimen.

In

other

words,

mictMcopic

examination

canbeconducted

immediately

after

manipulating

the

fine

adjustment

handle.

15

Focus

at

first

by

utilizing

lOX

objective.

The

distance

between

the

objective

and

the

microspecimeninthis

case

will

be

approximatelv

5.6mm.

Even

when

lOQX

objective

is

set

to

viewing

position.

it

will

not

collide

with

the

microspecimen_

The

distance

will

thenbeaboutO.t4mm.

INSTRUCTION

FOR

USING

OIL IMMERSION OBJECTIVE

Before

using

oil

immersion

objective.

secure

focus

by

using

low

magnifIcation

objective.

Apply

a

dropofcargille

011oranIsoletothe

surfaceofthe

specimen,

Also

apply

cargille

oiltothe

oil

immersion

objective_

As

we have already

mentioned,

the

oil

immersion

objective

will

not

touch

the

specimen

when

the

revolving

nosepiece

brings

it

into

position

over

the

specimen.

After

focusing

the

lensbymeansoftHe fine

adjustment

handle,

microscopic

examination

can be

carried

out

without

any

lossoftime.

After

examination.

cargille

oil

left

on

the

oil-

immersion

objective

must

be

thoroughly

wiped

off

with

gauze

dippedinxylene.

Otherwise.

the

lens

will

SOOn

become

useless, Crean

gauze

is

sufficient

to

wipe

off

anisole.

Place a

dropofcargille

oil

or

anisoleonthe

microspecimen

Apply

carsitle

oil

to

the

tip

of

the

oiHmmerson

oblctive

CAUTION

Microscopes

are

extremely

allergictomoisture

and

dust.

Since

both

moisture

and

dust

are

foundinmost

laboratories,

microscopes

should

be

kept

in

containers

imme:diately

after

use.

If

thisisnot

possible,

they

shouldbecovered

with

the

enclosed

vinyl

dust

cover.

As

for

objectives

and eyepieces.itis

best

to

keep

them

in

desiccators.

Falling

thls.

they

should

be

keptincases

containing

such

desiccants

as silica 8el.

After

the

e)'epiece has been

removed,

the

e,yeplece

tube

shouldbecovered

with

the

enclosed

cap.

Bynomeans

shouldamicroscopebetaken

apart

for

repairs.

This

should

be

lefttoexperts.

And,

of

COurse.

microscopes

must

alwa,ys be

kept

clean. Fine

dust

on

parts

that

cannot

be

reachedbythe

hand

shouldbeblownorwiped

off

by

meansofair

blowerorclean

feather.

16

ACCESSORIES

Small

Photomicrographic

Apparatus, PM-6

The

PM-6ISa

photomicrographic

unit

designed exclusively

for

taking

full-size

photographs

on

a

35mm

film. Fits all typesofbiological and metallur'gical microscopesofall makes.

o

Shutter

speed:

S,

1-1/50

sec.

o Size

of

image;

24mmx.36mm

o SpBcimen can be observed whlte

photographing

o Has

insertion

opening

(or

photo

cellofphotomicrographic

exposure meter'.

o Aio'allable

for

all eyepieces

except

5X

power.

o

Shock-proof

shutter

prevents

shutter

IJlbration and

insures

no

blurred

pictures.

o Magnification on the

film

plane:

about

If3Xoftotal

magnificationsofmicroscope.

Phase

Contrast

Equipment, PA •

PB

The

bright

field

observation

methodofmicroscopic

examination

can

not

be

used

for

examining

colorless,

transparent

specimens

since such

specimens

lack

contrast.

The phase

contrast

equipment,

therefore,

was designedtoprovide

such specimens

with

COntrast and thus

make

examination

of

tivlng

bacteria,

c-ells and

tissue

possible.

Two

kindsoflight

pass

through

specimen

namely,

undeviated

light

and

deviated

light.

Deviated

lIght

is

M wave

length

slower

than

undevi.ated

light.

Contrast

can be

strengthened

through

light

interferencebymakins

undeviated

light

!4-

!Nave

length

faster

or

srower. This

meth04

is

highly

convenient

for

observationotthe

ecologyofthe

specimen

sinceiteliminates

such

trou·

blesome

procedures

as

dyeing

the

specimen.

The

Olympus

phase

contrast

equipmentishighly

prized

today

sinceithas widened

the

scopeofbIologIcal

microscopic

e)(:amlnatlon. What is

more.

Itiseasytohandle.

Contents

of

Phase

Contrast

Equipment

Set.

model

PA

Phase

Contrast

Objectives

for

4

different

co-

ntrasts

each

with4different

magnifications

(total

of

16

objectives)

:

PL

lOX, 20X, 40X. 100X

(oil

Immerson)

PLL lOX, 20X, 40X, 100X

(oil

immerson)

NH

lOX, 20X, 40X,

IOOX

(oil

immerson)

NM

lOX, 20X, 40X, 100X

(oil

immerson)

Phase

contrast

turret

condenser,

Centering

Telescope

(CT),

Green

filter

(outer

diameter

32.5mm)

17

model

PB

One

setofPf1ase.

Contra-s!

Objectives

of

the

above

mentioned

4

contr.ast~

(PL,

PLL.1NH.

NMJ.

Als:J

contains

Ph;;lse

contrast

turret

condenser,

c.entering

telescope

(CT), and

green

filter

(outer

diameter

32.5mm).

Note;

Contr'3st;

P'L

Positive

Low

PLL

Positive

low low

NH

Negative

High

NM

Negative

Medium

Lens Container

ThiS

isacontainer

specially

made

for

miCfOScOPO

l@nS8S.

Withadesiccant

(silica

gel)atthe

bottom

of

the

container,

lenses

canbekept

completely

freeofmoisture

and

dust.

The

plastic

top

permits

to

check

the

lenses

from

outside

without

r'emolo'ing

the

top,

anditIs

very

convenient

to

keep

the

lenses in order"

Capacity:

7 lense'S

(upto57mm

hish)

3

filters

(upto33mm

in dia. and

3.5mm

thick)

Plan

Achromatic

Objectives

fllew and

originally

designed

Plan

Achromatic

Objectives,

completely

freeofspherical

aberration,

are

inCluded as

the

5tandard

setinthe

Olympus

Mic:roscope

FH-III,

Furthermore,

useofthe

low

magnification

Plan

Achromatic

Objectilo'es will help broaden

the

fieldofphotomicro,graphic

work.

o Plan

Achromatic

Objectives:

1.3X, 2X,

4X,

lOX,

20X,

40X,

lOOX

o Condenser

exclusively

for

low

magnificatioll

(1.3X

and 2X

Objectives)

microscopy.

18

Dark

field condenser

DC

8rownian

movement

of

celloid,

etc.

is hal'"dly

lJisiblll:!

in

bright

field,

but

with

aidofthe

dark

field

condenser, all

mic

ros-cope s

permit

examination

of

such mOVE!ment in a

dark

field.

(Use

lOOX

oil-immer$iOn

objective

with

iris

diaphragm

with

dark field

condenser.)

Filar

micrometer

eyepiece

OSM

Accurate

me.asurE!ment

ofaminimum

of

O.OOOlmm is

fe.asiblE!

by

Inserting

this

eyepiece

Into

the

tube

of

the

microcope.

When mOl.Jntlng

it

on

mOflOClJlar

inclined

he2ld.

binocular

head

Qr

trinoculal" head,

first

remoIJe

the

auxiliary

lens on the

bottom

~eetion,

and

insert

the

eye- piece

into

the

eyepiece sleeve,

Eyepiece

Micrometer

and Objective

Micrometer

These

are

usedtomeasure

the

sizeofspecimens.

(1)

Eyepiece

Micrometer:

A

round

Blass plate,

lOmm/100,

19mmindiameter'.

(2)

Objectl'ole

Micrometer;

A slide glass - 1mm/lOO.26x

76mm.

19

Photomicrographic

Exposuremeter, EMM·IV (equipped

with

CTR)

This

equIpment

is used

for

photomicrography

in

which

proper

exposure is

hardtodetermine.

ExpOsure failureS are especially

frequentIncolor

film

photomicrography;

$0

us/"

I;MM

- IV.

--:;'1

-

Polarizing

Attachment,

POL

ThiS

is

a

simptified

polarizing

apparatus

equipped

withapolarizing

condenser

including a

polarizer.

and 2l

polarizing

eyepiece including an analyzer. This can

be

attached

to

any

micro-

scope equipped

withacondenser

with

an

outer

diameterof36,8mm.

20