Ocean Optics USB2000 User Manual

USB2000
Fiber Optic Spectrometer

Installation and Operation Manual

Document Number 170-00000-000-02-1005

Offices:

E-mail: [e-mail] (General sales inquiries)

Ocean Optics, Inc.

830 Douglas Ave., Dunedin, FL., USA 34698

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Ocean Optics B.V. (Europe)

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Phone [phone]

F ax 31-(0)26-3190505

[e-mail] (European sales inquiries)
[e-mail] (Questions about orders)
[e-mail] (Technical support)

Copyright © 2001-2005 Ocean Optics, Inc.

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic,
mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.

This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hired out or
otherwise circulated without the prior consent of Ocean Optics, Inc. in any form of binding or cover other than that in which it is published.

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trademarks or trademarks of Microsoft Corporation.

Limit of Liability

Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The
information provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect
to any loss or damages arising from the information contained in this manual.

Table of Contents

1 USB2000 Introduction .....................................................................................................................1

Product Overview .................................................................................................................................................. 1

System Requirements...................................................................................................................................................................................1

EEPROM Utilization......................................................................................................................................................................................1

About OOIBase32.........................................................................................................................................................................................2

Sampling System Overview ..........................................................................................................................................................................2

Modular Sampling Accessories.....................................................................................................................................................................2

Interface Options ................................................................................................................................................... 3

Shipment Components..........................................................................................................................................3

Packing List...................................................................................................................................................................................................3

Wavelength Calibration Data Sheet..............................................................................................................................................................3

Software and Technical Resources CD ........................................................................................................................................................4

Other Documentation....................................................................................................................................................................................4

Modification and Repair Policy......................................................................................................................................................................4

2 USB2000 Specifications..................................................................................................................5

How the USB2000 Works...................................................................................................................................... 5

USB2000 Component Table .........................................................................................................................................................................6

USB2000 Specifications........................................................................................................................................ 7

CCD Detector Specifications.........................................................................................................................................................................7

USB2000 Spectrometer Specifications.........................................................................................................................................................7

System Compatibility.....................................................................................................................................................................................8

10-pin Accessory Connector Pinout..............................................................................................................................................................8

10-Pin Accessory Connector Pinout Diagram...............................................................................................................................................9

10-Pin Accessory Connector – Pin Definitions..............................................................................................................................................9

10-Pin J2 Accessory Connector – Part Number and Compatibility...............................................................................................................9

3 Installing the USB2000..................................................................................................................10

USB2000 Installation........................................................................................................... ................................10

Configuring the USB2000 in OOIBase32....................................................................................................................................................11

Connect Spectroscopic Accessories...........................................................................................................................................................13

External Triggering Options................................................................................................................................. 13

4 Troubleshooting ............................................................................................................................14

USB2000 Connected to PC Prior to OOIBase32 Installation.............................................................................. 14

Remove the Unknown Device from Windows Device Manager..................................................................................................................14

Windows 98:................................................................................................................................................................................................15

Windows 2000/XP:......................................................................................................................................................................................16

Troubleshooting the Serial Port Configuration..................................................................................................... 17

Older Version of OOIBase32 Installed ................................................................................................................ 17

5 Sample Experiments .....................................................................................................................18

Preparing for Experiments................................................................................................................................... 18

Absorbance Experiments..................................................................................................................................... 20

Transmission Experiments .................................................................................................................................. 22

Reflection Experiments........................................................................................................................................24

Relative Irradiance Experiments.......................................................................................................................... 26

Time Acquisition Experiments ............................................................................................................................. 28

Appendix A: Calibrating the Wavelength of the USB2000............................................................31

About Wavelength Calibration............................................................................................................................. 31

Calibrating the Spectrometer............................................................................................................................... 31

Preparing for Calibration .............................................................................................................................................................................31

Calibrating the Wavelength of the Spectrometer.........................................................................................................................................32

Saving the New Calibration Coefficients: USB Mode..................................................................................................................................34

Saving the New Calibration Coefficients: Serial Mode................................................................................................................................35

Appendix B: USB2000-FLG Spectrometer.........................................................................................36

About the USB2000-FLG..................................................................................................................................... 36

Using the USB2000-FLG..................................................................................................................................... 36

Index ......................................................................................................................................................38

USB2000 Introduction

1 USB2000 Introduction

The following chapter contains introductory information about the USB2000 Spectrometer, shipment information,
and spectrometer connectivity.

Product Overview

The USB2000 Spectrometer connects to a notebook or desktop PC via USB port or serial port. When connected
to the USB port of a PC, the USB2000 draws power from the host PC, eliminating the need for an external power
supply.

Figure 1-1: Ocean Optics USB2000 Fiber Optic Spectrometer

System Requirements

You can use the USB2000’s USB connectivity with any PC that meets the following requirements:

• Windows 98/Me/2000/XP operating system (or Windows CE 2.11 or later for palm-sized PCs)

• Ocean Optics OOIBase32 software application installed and configured for use with the USB2000

(OOIPS2000 software if using a palm-sized PC). Consult the “Configuring the USB2000 in OOIBase32”
section of Chapter 3: Installing the USB2000 for specific configuration instructions.

Alternately, the USB2000 has a serial port for connecting to PCs, PLCs, and other devices that support the RS­232 communication protocol. However, this connection method requires an external power supply to power the
USB2000.

EEPROM Utilization

An EEPROM memory chip in each USB2000 contains wavelength calibration coefficients, linearity coefficients,
and a serial number unique to each individual spectrometer. The OOIBase32 software application reads these
values directly from the spectrometer, enabling the ability to “hot-swap” spectrometers between PCs without
entering the spectrometer coefficients manually on each PC.

USB2000 Operating Instructions 1

USB2000 Introduction

About OOIBase32

OOIBase32 is the latest generation of operating software for all Ocean Optics spectrometers and is available free
to all customers. OOIBase32 is a user-customizable, advanced acquisition and display program that provides a
real-time interface to a variety of signal-processing functions. With OOIBase32, you have the ability to perform
spectroscopic measurements (such as absorbance, reflectance, and emission), control all system parameters,
collect and display data in real time, and perform reference monitoring and time acquisition experiments.

Note: When using a palm-sized PC, you will use the OOIPS2000 software instead of OOIBase32. The

functionality of OOIPS2000 is similar to OOIBase32, but it is specifically for the palm-sized PC.

Sampling System Overview

Ocean Optics fiber optic spectrometer systems consist of low-cost, modular data acquisition components. A
typical USB2000-based sampling system contains four core elements:

• USB2000 Spectrometer

• OOIBase32 operating software

• Light source

• Sampling optics (varying, depending on application requirements)

How Sampling Works

The following list explains the function of Ocean Optics sampling components in the sampling process:

1. The user stores reference and dark measurements to correct for instrument response variables.

2. The light from the light source transmits through an optical fiber to the sample.

3. The light interacts with the sample.

4. Another optical fiber collects and transmits the result of the interaction to the spectrometer.

5. The spectrometer measures the amount of light and transforms the data collected by the spectrometer
into digital information.

6. The spectrometer passes the sample information to OOIBase32.

7. OOIBase32 compares the sample to the reference measurement and displays processed spectral
information.

Modular Sampling Accessories

Ocean Optics offers a complete line of spectroscopic accessories for use with the USB2000. Most of our
spectroscopic accessories have SMA connectors for application flexibility. Accordingly, changing the sampling
system components is as easy as unscrewing a connector and replacing an accessory.

USB2000 Operating Instructions 2

USB2000 Introduction

Interface Options

The USB2000 has both USB and serial port connectors, enabling you to connect the spectrometer to a desktop or
notebook PC via a USB port or to a desktop, notebook, or to a palm-sized PC via a serial port.

Computer

Interface

Desktop or
Notebook PC
via USB Port

Desktop or
Notebook PC
via Serial Port

Palm-sized PC
via Serial Port

Operating System

Requirements

Windows 98/Me/
2000/XP

Any 32-bit
Windows operating
system

Windows CE 2.11
or higher

Part Needed Description of Part

USB-CBL-1
(included)

USB-ADP-PC
(not included)

USB-ADP-H
(not included)

Cable that connects from USB port on USB2000 to USB port on
desktop or notebook PC

Adapter block that enables connection from serial port on
USB2000 to serial port on desktop or notebook PC; comes with
5 VDC power supply (required when connecting to serial port)

Adapter block that enables connection (with standard 9-pin
serial cable) from serial port on USB2000 to serial port on palm­sized PC; comes with 5 VDC power supply (required when
connecting to serial port)

Shipment Components

The following information and documentation ships with the USB2000 Spectrometer:

Packing List

The packing list is inside a plastic bag attached to the outside of the shipment box (the invoice arrives separately).
The packing slip lists all items in the order, including customized components in the spectrometer (such as the
grating, detector collection lens, and slit). The packing list also includes the shipping and billing addresses, as well
as any items on back order.

Wavelength Calibration Data Sheet

Each spectrometer is shipped with a Wavelength Calibration Data Sheet that contains information unique to your
spectrometer. OOIBase32 Operating Software reads this calibration data from your spectrometer when it
interfaces to a PC via the USB port. Any other interface requires that you manually enter the calibration data in
OOIBase32 (select Spectrometer | Configure | Wavelength Calibration tab). See the OOIBase32
documentation for more information (refer to Product Overview
documentation).

Note: Please save the Wavelength Calibration Data Sheet for future reference.

USB2000 Operating Instructions 3

for instructions on accessing OOIBase32

USB2000 Introduction

Software and Technical Resources CD

Each order ships with the Ocean Optics Software and Technical Resources CD. This disc contains software,
operating instructions, and product information for all Ocean Optics software, spectrometers, and spectroscopic
accessories. You need Adobe Acrobat Reader version 6.0 or higher to view these files. Ocean Optics includes
Adobe Acrobat Reader on the Software and Technical Resources CD.

With the exception of OOIBase32 Spectrometer Operating Software, all Ocean Optics software requires a
password during the installation process. You can locate passwords for the other software applications on the
back of the Software and Technical Resources CD package.

Other Documentation

You can find detailed instructions for the OOIBase32 Spectrometer Operating Software at
http://www.oceanoptics.com/technical/operatinginstructions.asp.

Note: You can find instructions for the OOIPS2000 Operating Software for the handheld PC at the following

location: http://www.oceanoptics.com/products/ooips2000.asp.

To find operating instructions on some of Ocean Optics’ most popular spectroscopic accessories (including light
sources, sampling chambers, sampling optics, and probes), consult the operating instructions for Ocean Optics
products from the Software and Technical Resources CD that ships with the product, or visit our website at
http://www.oceanoptics.com/technical/operatinginstructions.asp.

Modification and Repair Policy

In the event that you need to return an item for modification or service, you must obtain a Return Merchandise
Authorization (RMA) number prior to shipping the item back to Ocean Optics. Contact an Ocean Optics
Applications Scientist for specific instructions on returning an item.

USB2000 Operating Instructions 4

USB2000 Specifications

2 USB2000 Specifications

This chapter contains information on spectrometer operation, specifications, and system compatibility. It also
includes accessory connector pinout diagrams and pin-specific information.

How the USB2000 Works

The following diagram illustrates the movement of light through the optical bench of the USB2000 Spectrometer.
You can customize the items marked with an asterisk (*) when ordering the USB2000.

Note: The optical bench has no moving parts that can wear or break. Ocean Optics secures all components in

place permanently at the time of manufacture.

Figure 2-1: USB2000 Spectrometer with Components

The USB Component Table on the following page explains the function of each numbered component in the
USB2000 Spectrometer diagram (Figure 2-1).

USB2000 Operating Instructions 5

USB2000 Specifications

USB2000 Component Table

Ocean Optics permanently secures all components in the USB2000 at the time of manufacture. Only Ocean
Optics Technicians can replace interchangeable components, where noted.

Item Name Description

1 SMA Connector

2 Slit*

3 Filter*

4 Collimating Mirror

5 Grating*

6 Focusing Mirror

L2 Detector

7

Collection Lens*

CCD Detector

8

(UV or VIS)

The SMA Connector secures the input fiber to the spectrometer. Light from the input fiber enters the optical bench through this connector.

The Slit is a dark piece of material containing a rectangular aperture, which is
mounted directly behind the SMA Connector. The size of the aperture regulates the
amount of light that enters the optical bench and controls spectral resolution.

You can also use the USB2000 without a Slit. In this configuration, the diameter of
the fiber connected to the USB2000 determines the size of the entrance aperture.

Only Ocean Optics technicians can change the Slit.
The Filter is a device that restricts optical radiation to pre-determined wavelength

regions. Light passes through the Filter before entering the optical bench. Both
bandpass and longpass filters are available to restrict radiation to certain
wavelength regions.

Only Ocean Optics technicians can change the Filter.
The Collimating Mirror focuses light entering the optical bench towards the Grating

of the spectrometer.
Light enters the spectrometer, passes through the SMA Connector, Slit, and Filter,

and then reflects off the Collimating Mirror onto the Grating.
The Grating diffracts light from the Collimating Mirror and directs the diffracted light

onto the Focusing Mirror. Gratings are available in different groove densities,
allowing you to specify wavelength coverage and resolution in the spectrometer.

Only Ocean Optics technicians can change the Grating.
The Focusing Mirror receives light reflected from the Grating and focuses the light

onto the CCD Detector or L2 Detector Collection Lens (depending on the
spectrometer configuration).

The L2 Detector Collection Lens (optional) attaches to the CCD Detector. It
focuses light from a tall slit onto the shorter CCD Detector elements.

The L2 Detector Collection Lens should be used with large diameter slits or in
applications with low light levels. It also improves efficiency by reducing the effects
of stray light.

Only Ocean Optics technicians can add or remove the L2 Detection Collection
Lens.

The CCD Detector collects the light received from the Focusing Mirror or L2
Detector Collection Lens and converts the optical signal to a digital signal.

Each pixel on the CCD Detector responds to the wavelength of light that strikes it,
creating a digital response. The spectrometer then transmits the digital signal to the
OOIBase32 application.

USB2000 Operating Instructions 6

USB2000 Specifications

USB2000 Specifications

The following sections provide specification information for the CCD Detector in the USB2000, as well as the
USB2000 Spectrometer itself.

CCD Detector Specifications

Detector:
Number of elements:
Pixel size:
Pixel well depth:
Signal-to-noise ratio:
A/D resolution:
Dark noise:
Corrected linearity:

Sony ILX511 linear silicon CCD array
2048 pixels
14 µm x 200 µm
62,500 electrons
250:1 (at full signal)
12 bit

2.5 RMS counts
>99.8%

USB2000 Spectrometer Specifications

Dimensions:
Weight:
Power consumption:
Detector range:
Detector:
Gratings:
Entrance aperture:
Order-sorting filters:
Focal length:
Optical resolution:
Dynamic range:
Stray light:

Sensitivity (estimate):
Fiber optic connector:

Data transfer rate:
Integration time:
Fiber optic connector:

Operating systems:

89.1 mm x 63.3 mm x 34.4 mm
190 g (without cable)
90 mA @ 5 VDC
200-1100 nm
2048-element linear silicon CCD array
14 gratings; UV through Shortwave NIR
5, 10, 25, 50, 100 or 200 mm wide slits or fiber (no slit)
Installed longpass and bandpass filters
f/4, 42 mm (input); 68 mm (output)
~0.3-10.0 nm FWHM (depending on grating and size of entrance aperture)

8

2 x 10
<0.05% at 600 nm; <0.10% at 435 nm; <0.10% at 250 nm
400 nm – 90 photons/count; 600 nm – 41 photons/count;

800 nm – 203 photons/count
SMA 905 to single-strand optical fiber (0.22 NA)
Full scans into memory every 13 milliseconds
3 milliseconds to 65 seconds
SMA 905 to single-strand optical fiber (0.22 NA)
Windows 98/Me/2000/XP when using the USB interface on a desktop or notebook

PCs
Any 32-bit Windows operating system when using the serial port on desktop or

notebook PCs
Windows CE 2.11 and above when using the serial port on palm-sized PCs

(system); 2000:1 for a single scan

USB2000 Operating Instructions 7

USB2000 Specifications

System Compatibility

The following sections provide information on hardware and software requirements for the USB2000:

Compatibility for Desktop or Notebook PCs

To use the USB2000, you must have a PC that meets the following minimum requirements:

• IBM-compatible PC with Pentium (or higher) processor

• 32 MB RAM

• OOIBase32 Spectrometer Operating Software

• Windows 98/Me/2000/XP operating system (when connecting the USB2000 to a PC via USB port)

or

Any 32-bit version of Windows (when connecting the USB2000 to a PC via serial port)

Compatibility for Palm-sized PCs

In order to use the USB2000 with your palm-sized PC, the computer must meet the following requirements:

• Palm-sized PC running Windows CE 2.11 or higher

• 32 MB

• OOIPS2000 Spectrometer Operating Software

• Serial port connectivity

10-pin Accessory Connector Pinout

The USB2000 features a 10-pin Accessory Connector, located on the side of the unit as follows:

10-pin Connector

Figure 2-2: Location of USB2000 10-pin Connector

USB2000 Operating Instructions 8