Ocean Optics OOIBase16 User Manual

OOIBase Operating
Software

Instruction Manual

Version 1.52

Ocean Optics, Inc.
380 Main Street
Dunedin, FL 34698
[phone]
[phone] fax

For the latest information and software upgrades
consult our web site:
www.OceanOptics.com

Or, e-mail our Technical Service Department:
[e-mail]

070699

Contents

About OOIBase Operating Software

Installing OOIBase Operating Software

Using OOIBase Operating Software

File Menu Functions .........................................................................................3

View Menu Functions .......................................................................................4

Setup Menu Functions......................................................................................6

Toolbar Functions.............................................................................................9

Doing an Experiment

Absorbance Measurement..............................................................................10

Transmission Measurement............................................................................11

...................................................................................10

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Relative Irradiance Measurement...................................................................11

Reflection Measurement.................................................................................12

About OOIBase Operating Soft ware

OOIBase is the standard operating software for Ocean Optics spectrometers. OOIBase for Windows is a
basic acquisition and display software that provides a real-time interface to a variety of signal-processing
functions. OOIBase allows users to perform basic spectroscopic measurements such as absorbance,
reflectance and emission, as well as more sophisticated tasks such as the automation of experiments with a
time-series acquisition function. OOIBase allows the user to view, in real-time and entirely on one screen,
spectral data from up to 8 spectrometer channels.

Absorbance spectrum of cranberry juice

OOIBase offers users considerably more flexibility than they are likely to experience with other spectrometer
operating software. The basic concept for the software is that real-time display of data allows users to
evaluate the effectiveness of their experimental setup and data processing selections, make changes to these
parameters, instantly see their effect, and save the data. OOIBase offers:

! Spectrometer control

• integration time regulation

• multiple triggering options

• channel selection

! Signal Processing including:

• signal averaging

• boxcar pixel smoothing

• electrical dark-signal subtraction

• stray light correction

! Spectral Processing: absorbance, transmission, irradiance
! Time series acquisition for full spectra
! Real time graphical display with zoom, cursor and cosmetic selections
! Multi-window support
! File Export to ASCII format

OOIBase operates with Windows 3.x and Windows 95/98. Windows NT users are easily able to write their
own applications using the OOIWinIP Windows or OOILVD La bVIEW drivers.

All Ocean Optics software products comply with Year 2000 requirements.

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Installing OOIBase Operating Soft ware

1. Insert the OOIBase floppy disk into a drive.

2. Select

3. Click OK at the “Welcome!” dialog box.

4. Select the

5. In the “Make Backups” dialog box, click

6. After the software installation is complete, you will go back out to your desktop.

7. Start OOIBase by selecting

8. The first time the software starts, the “Interface Setup Parameters” dialog box opens. This box allows

Run

and type

Destination Directory

housed. Clic k OK.

you to specify your Spectrometer, A/D Converter, Base Address (I/O Range), and IRQ (Interrupt
Request). Choose the correct parameters for your setup. For the ADC500, ADC1000 or PC2000,
choose the same Base Address and IRQ settings as the dip switches on the A/D board. If you left them
unchanged, accept the default settings (Base Address of 768 and IRQ of 7). If you have the DAQ-700,
you will have to use your computer to find the correct settings of the hardware. For specific
instructions on changing the settings for these A/D cards, see the directions that came with your
product. If you have the SAD500, ignore the Base Address and IRQ selections. Click OK. You can
always go back to this dialog box by selecting

(drive):setup

. Click OK.

. Choose the default destination or type where you want the software

YES

.

Start | Programs | OOIBase V 1.5 | OOIBase V 1.5

Setup | Configure Hardware

.

9. If you do not see the “Interface Setup Parameters” dialog box when you start OOIBase, exit OOIBase
by selecting
open Notepad and allow you to edit the initialization file for our device driver. Find the “Initialized”
entry within this file. Make sure this line reads: Initialized=0. Save the OOIDRV.INI file and
exit Notepad. Restart OOIBase. You should now see the “Interface Setup Parameters” dialog box
allowing you to spec i f y your ha rdware configurat io n.

10. At this point, OOIBase should be acquiring data from your spectrometer. There should be a dynamic
trace responding to light near the bottom of the displayed graph. If your spectrometer is not responding
to light (or your computer hangs or crashes), please contact our Technical Support Department.

11. Exit and restart OOIBase. This enables the hardware changes to take effect.

File | Exit

. Then select

Start | Run

, and type C:\windows\ooidrv.ini. This will

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Using OOIBase Operating Soft ware

File Menu Functions

New

This command opens a new window and starts acquiring data from the spectrometer. By default, the Master
channel is selected and the graph is placed in Scope Mode.

Open

This command opens a dialog box that allows you to reload a saved set of spectra. If you saved the dark or
the reference spectra, they are also restored.

Close

This command immediately closes the current graph window. You are
current spectrum when this command is chosen.

Store Dark Spectrum

This command stores the current spectrum as the dark spectrum for the channel activated. This spectrum is
taken with the light source off or with the light path blocked. (See
Saving a dark spectrum is requisite before the software can make the calculations of absorbance,
transmission, and relative irradiance spectra.

not

given the opportunity to save the

Doing an Experiment

on page 15.)

Store Reference Spectrum

This command stores the current spectrum as the reference spectrum for the channel activated. This
spectrum is taken with the light source on and a blank in the sampling region. (See
on page 15.) Saving a reference spectrum is requisite before the software can make the calculations of
absorbance, transmission, and reflection spectra.

Doing an Experiment

Save Sample Spectrum

This command opens a dialog box that allows you to save a set of spectra as a binary file. If the dark and
reference spectra are saved, they are also saved in the binary file. Data acquisition parameters are also saved.

Export Spectrum

This command opens a dialog box that allows you to export the current spectra (sample, reference, and/or
dark) into a comma-delimited ASCII file. You are given the opportunity to specify the filename. The
following file extensions are the default:

Spectrum Extension

Reference .REF
Dark .DRK
Sample

Absorbance .ABS
Transmission .TRM
Reflection .RFL
Scope .SCO

Print Preview

This command opens a window allowing you to view how the current spectrum will look when printed.

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