Leica Premium blade holder User Manual

Instructions for Use

Leica Premium blade holder
Version 1.3, English – 10/2012
Order number: 14 0491 82101, RevD
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Only valid together with the Instructions for Use of the
delivered cryostat.
All notes in the cryostat's Instructions for Use apply.

Premium

blade holder

NOTE

The information, numerical data, notes and value
judgments contained in this manual represent
the current state of scientific knowledge and
state-of-the-art technology as we understand it
following thorough investigation in this field. We
are under no obligation to update the present
manual periodically and on an ongoing basis ac­cording to the latest technical developments, nor
to provide our customers with additional copies,
updates etc. of this manual.
To the extent permitted in accordance with the
national legal system as applicable in each indi­vidual case, we shall not be held liable for errone­ous statements, drawings, technical illustrations
etc. contained in this manual. In particular, no
liability whatsoever is accepted for any financial
loss or consequential damage caused by or
related to compliance with statements or other
information in this manual.
Statements, drawings, illustrations and other in­formation as regards contents or technical details
of the present Instructions for Use are not to be

considered as warranted characteristics of our

products. These are determined only by the con­tract provisions agreed between ourselves and
our customers.
Leica reserves the right to change technical spec­ifications as well as manufacturing processes
without prior notice. Only in this way is it pos­sible to continuously improve the technology and
manufacturing techniques used in our products.
This document is protected under copyright laws.
All copyrights to this documentation are held by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or
of any parts thereof) by means of print, photo­copy, microfiche, web cam or other methods—
including any electronic systems and media—
requires express prior permission in writing by
Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the nameplate on
the back of the instrument.

© Leica Biosystems Nussloch GmbH

Published by:
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
69226 Nussloch
Germany
Phone: [phone]
Fax: [phone]
Internet: http://www.LeicaBiosystems.com

Leica Premium blade holder

3

Contents

1. Sectioning – Premium blade holder ..................................................................................................... 5

Inserting the base .................................................................................................................................... 5

Inserting the premium blade holder ...................................................................................................... 5

Assembling the anti-roll system ............................................................................................................ 5

Inserting blades into premium blade holder ........................................................................................ 6

Inserting high-profile blades .................................................................................................................. 6

Inserting low-profile blades ................................................................................................................... 6

Removing the blades ............................................................................................................................... 7

Lateral movement ..................................................................................................................................... 8

Clearance angle adjustment .................................................................................................................. 9

2. Optional components ............................................................................................................................ 10

Premium blade holder (for low-profile and high-profile blades) with anti-roll system .............. 10

Assembling the anti-roll system for the Premium blade holder ..................................................... 10

Premium blade holder (for low-profile and high-profile blades) with finger rest ........................ 11

Conversion of blade holder with anti-roll system to blade holder with finger rest ..................... 11

Adjusting blade holder with anti-roll system ..................................................................................... 12

3. Daily cleaning of the premium blade holder .................................................................................... 13

4

Instructions for Use V1.3 – 10/2012

1. Sectioning – Premium blade holder

Inserting the base

1. To insert the blade holder base (1), move the
lever (2) to the back. Slide the base onto the
T-piece (3) of the baseplate.

23

2

1

3

When inserting the blade holder base,
you have to overcome the resistance
of the spring (located in the base of the
blade holder).

2. To clamp the blade holder base, turn the lever

Fig. 1

(2) to the front.

Inserting the premium blade holder

1. Push the segment arc onto the base.

2. Clamp the blade holder into place on the left
side with a 4 mm Allen key (23). In doing so,
take the desired clearance angle into account
(left scale 0°–10°).

Assembling the anti‑roll system

Anti-roll systems for section thicknesses of 50 µm
and 100 µm are included in the standard deliv­ery. Anti-roll systems for section thicknesses of
150 µm are available as an option. For details on
how to assemble the anti-roll system, see chapter
“Assembling the anti-roll system for the Premium
blade holder“ on page 10.

Leica Premium blade holder

Fig. 2

5