Leica LED1, LED3, LED8, LED5 Instructions For Use Manual
From Eye to Insight
LEICA LED1
LEICA LED3
LEICA LED5
LEICA LED8
External light sources for Leica microscopes
Instructions for use
Leica Microsystems CMS GmbH. Instructions for use, 11934173, Revision 1.0, 2019.05.01
Document Number 57-10022 Revision A
1
Copyright
Copyright
All rights to this documentation are held by
Leica Microsystems CMS GmbH. Reproduction
of text or illustrations (in whole or in part) by
print, photocopy, microfilm or other method
(including electronic systems) is not allowed
without express written permission from Leica
Microsystems CMS GmbH.
The instructions contained in the following documentation reflect state-of-the-art technology and
knowledge standards. We have compiled the
texts and illustrations as accurately as possible.
Nevertheless, no liability of any kind shall be accepted for the accuracy of this manual's contents. Still, we are always grateful for comments
and suggestions regarding potential errors within
this documentation.
The information included in this manual may be
changed without prior notice.
As the official manufacturer of Leica branded
light engine products, Lumencor is the responsible owner of this instructions for use 57-10022,
Revision A.
Leica Microsystems CMS GmbH is the distributor
of the Lumencor illumination products detailed
herein, 11934173, Revision 1.0 2019.05.01 is for
Leica internal tracking purposes only.
Revision 1.0, edited 2019.05.01 by:
Leica Microsystems CMS GmbH
Ernst-Leitz-Strasse 17–37
D-35578 Wetzlar (Germany)
http://www.leica-microsystems.com
Responsible for contents:
Marketing CMS
2
Contents
Contents
1. Important Notes about this Manual .............4
2. Function of the Compact Light Source ........6
3. Safety Notes .....................................................7
3.1 Regulatory safety notes .................................7
3.1.1 Introduction LED1 ............................................8
3.1.2 Introduction LED3 ............................................8
3.1.3 Introduction LED5/LED8 ..................................8
3.1.4 Precautions and Warnings ...........................9
3.2 Electrical safety .............................................12
3.3 Disposal ..........................................................15
4. Transport, Storage, Unpacking ..................16
5. Notes on Warranty ........................................17
6. Overview of the Instrument .........................18
6.1 Intended use ..................................................18
6.2 Identification of the instrument ...................18
6.3 Special features of the instrument .............18
6.4 Overview Light Sources ...............................19
6.5 Connecting LED1 ...........................................23
6.6 Connectable light guides (LED3-8) .............23
7. Instrument Setup ...........................................24
7.1 Installation ......................................................24
7.2 Connecting the LED1 .....................................24
7.3 LED3/5/8 – connecting the light guide .......24
7.4 Connecting the Leica POD (optional) .........26
7.5 Connecting the power cable .......................26
8. Operation ........................................................27
8.1 Operation LED1 ..............................................27
8.2 Operation LED3 ..............................................27
8.3 Operation LED5/8 ...........................................29
8.3.1 Start Up ...........................................................30
9. Service ............................................................31
9.1 Maintenance and cleaning ..........................31
9.2 Repairs ............................................................31
10. Troubleshooting ............................................32
11. EC Declaration of Conformity .....................33
3
1. Important Notes about this Manual
1. Important Notes about this Manual
Attention!
This User Manual is an essential component
of the instrument and must be read carefully
before assembly, commissioning or use.
Text symbols, pictograms and their meanings:
This User Manual contains important instructions and information for the operational safety
and maintenance of the instrument and accessories. Therefore, it must be kept in a safe place.
(1.2)
→ page 20
WARNING
CAUTION
!
Numbers in parentheses refer to illustrations; for
example, (1.2) refers to Fig. 1, item 2.
Numbers with a pointer arrow (for example →
page 20), point to a certain page of this manual.
WARNING indicates a potentially hazardous
situation with a medium degree of risk that, if
not avoided, could result in death or serious
injury.
CAUTION indicates a potentially hazardous situation with a low degree of risk that, if not avoided,
may result in minor or moderate injury.
Attention!
In this manual, additional safety notes are indicated with the triangle symbol shown here,
and have a gray background.
Attention! The instrument and its accessories
can be damaged when operated incorrectly.
4
1. Important Notes about this Manual
Warning of hazardous electrical voltage! Danger
of electric shock!
Warning of hot surface.
Warning: Possibly hazardous optical radiation
emitted from this product. Do not look at operating lamp. Eye injury may result.
UV
Warning: UV emitted from this product. Avoid eye
and skin exposure to unshielded product.
Warning: IR emitted from this product. Do not
IR
look at operating lamp.
Warning of electromagnetic field
Instructions for disposing of the instrument,
its accessories and consumables.
Earth (Ground) terminal
Explanatory note.
*
Item not contained in all configurations.
MM/YYYY
Manufacturer's address with manufacturing date
China RoHS 50 years EFUP
(Environmentally friendly use period)
5
2. Function of the Compact Light Source
2. Function of the Compact Light Source
The Leica LED1, LED3, LED5 and LED8 compact
light sources, with which this instructions for use
are associated, are intended for biological routine and research work as well as examinations
in materials science or mineralogy.
Reasonably foreseeable misuse
The following are prohibited:
• Using the light source for any purpose not in
accordance with the Declaration of Conformity
(e.g. use as a medical product in accordance
with EU Directive 93/42/EEC).
• Cleaning the light source in a way other than
specified in the manual.
• Modifying the built-in safety circuits in the instrument.
• Allowing unauthorized personnel to open the
instrument.
• Using cables that Leica has not provided or
permitted.
• Using combinations with non-Leica components that go beyond the scope of the User
Manual.
• Operating the light source while it is not connected to the stand.
The manufacturer assumes no liability for
damage caused by, or any risks arising from,
using the light source for other purposes than
those for which they are intended or not using them within the specifications of Leica
Microsystems CMS GmbH.
In such cases, the Declaration of Conformity
shall be invalid.
This instrument is not intended for use in a
patient environment as defined by DIN VDE
0100-710. It is also not intended to be combined with medical devices as defined by
EN 60601-1. If a microscope is electrically
connected to a medical instrument in accordance with EN 60601-1, the requirements defined in EN 60601-1-1 shall apply.
Attention!
Attention!
6
3. Safety Notes
3. Safety Notes
3.1 Regulatory safety notes
Leica Microsystems utilizes regulatory model
names for all certified and CE marked products.
The regulatory model names are traceable to
all regulatory documentation, third party reports
and certifications.
“Regulatory Model: LED1/LED3/LED5/LED8 is
used as a representative model for all certified
and CE marked LED1/LED3/LED5/LED8 Products.
Emissions
This equipment has been tested and found to
comply with the limits of EMC directive 2014/30/
EU. These limits are designed to provide reasonable protection against harmful interference
when the equipment is operated in a commercial
environment. This equipment generates, uses,
and can radiate radio frequency energy and, if
not installed and used in accordance with the
instruction manual, may cause harmful interference to radio communications.
It also meets the requirements of EN 61326-1,
Electrical equipment for measurement, control
and laboratory use – EMC requirements.
Photobiological Safety
Safety Certifications
TÜV SÜD America, CB Certification
(IEC 61010-1:2010)
TÜV SÜD America, NRTLus Certification
(UL 61010-1:2012)
TÜV SÜD America, cNRTL Certification
(CAN/CSA-C22.2 No. 61010-1:2012)
TÜV SÜD America, EN Certification
(EN 61010-1:2010)
CE Marking
Low Voltage Directive (2014/35/EU)
EMC Directive (2014/30/EU)
RoHS Directive (2011/65/EU) plus (2015/863/EU)
REACH Regulation (EC) No. (1907/2006/EC)
In accordance with the REGULATION (EU) No.
1194/2012 OF THE COMMISSION for the implementation of the Directive 2009/125/EC of the
European Parliament and Council with regard to
the requirements for the environmentally friendly
design of lamps with highly focused light, LED
lamps and associated instruments, the products
described here, Leica LED1, LED3, LED5 and
LED8, are special products because they are not
suitable for general lighting purposes.
The compact light sources have also been tested
in accordance with EN 62471 / IEC 62471 Photobiological safety of lamps and lamp systems and
is classified in risk group 3 (high risk).
7
3. Safety Notes
3.1.1 Introduction LED1
The LED1 light source is a compact solid-state
light source for fluorescence microscopy and
other bioanalytical applications. The light source
incorporates efficient thermal management, requiring no fan and providing quiet and vibrationfree operation. This enables direct coupling to
the fluorescence epi-illumination port of a microscope.
The light source provides four distinct color
bands of light output that are controlled using
either manual control knobs on the side panel
or the light source control POD accessory. The
COLOR knob located on the side panel is used for
on/off control, selection of color channels and for
enabling external tablet control. The INTENSITY
knob provides four different levels of output attenuation between 100% and 10% of maximum.
3.1.2 Introduction LED3
The LED3 light sources are designed for laboratory use by bioanalytical researchers and/
or developers of life science instrumentation.
The light sources generate white light output by
combining the outputs of multiple solid state light
sources. The light output on/off status is manually controlled via a rocker switch located on the
front panel or a foot pedal toggle switch accessory that plugs into the 3.5 mm connector on the
rear panel.
3.1.3 Introduction LED5/LED8
The LED light source consists of 4 (LED5) and 8
(LED8) individually addressable solid-state light
sources with integrated electronic control systems. The constituent light sources may be LEDs
or luminescent light pipes.
The outputs of the constituent light sources are
refined by bandpass filters and merged into a
common optical train directed to the light output
port on the front panel. The light output port has
a built-in adapter for connection to a liquid light
guide (LLG). An onboard photodiode continuously
monitors the light output and generates reference signals that can be applied to the constituent sources in a feedback loop to maintain constant light output over time.
The LED5/8 light sources are controlled by onboard micro-processors that connect to LAS X
imaging software via a RS-232 (PC) serial interface. The user can enable or disable each
source independently by serial commands, as
well as change the intensity of each source independently. Alternatively, the light sources may
be turned on and off by TTL inputs from a trigger
device such as a camera or a real-time controller. The only manual control is the master power
switch on the front panel. Optimal internal operating temperature is maintained by negative
pressure air cooling with the air intake at the
front of the light engine and the exhaust fan at
the the rear.
8
3. Safety Notes
3.1.4 Precautions and Warnings
A few simple practices will ensure trouble-free
operation for the life of the light engine.
Safety Instructions:
Please read and follow all safety instructions
provided BEFORE using the light source. Failure
to comply with the safety instructions may result
in fire, electrical shock, or personal injury and
may damage or impair protection provided by
equipment. Please save all safety instructions.
In order to maintain this condition and to ensure
safe operation, the user must follow the instructions and warnings contained in this User Manual.
Safety Definitions
Warning: Statements identify conditions or practices that could result in personal injury
Warning: DO NOT look into the output of the
light engine. The brightness of this light source
is higher than most commercial lighting fixtures
and is intended to couple directly into a microscope or other bioanalytjcal instrument.
Warning: DO NOT turn on the light without the
output end of the light guide safely directed
into an enclosed optical path. DO NOT point the
light output directly onto any flammable or burnsusceptible material. This includes all animal or
vegetable tissues, plastics, fabrics, paper and
liquids
RISK GROUP 3
Applicable for LED1/3/5/8
RISK GROUP 3
WARNING Possibly hazardous optical
radiation emitted from this product.
Do not look at operating lamp.
Eye injury may result.
Groupe de risque 3
AVERTISSEMENT Ce produit émet un
rayonnement optique potentiellement
dangereux. Ne regardez pas la lampe
lorsqu‘elle est allumée. Des lésions
oculaire peuvent en résulter.
Caution: Statements identify conditions or practices that could result in damage to your equipment.
Safety Items
Warning: ONLY use the power supply provided
by Leica. It is imperative that the DC power supply has output over-current protection, as the
power input of the light sources are not fused.
The DC power supply must have the AC power
cord connected to a receptacle with a protective
safety (earth) ground terminal
Applicable for LED3/5/8
WARNING IR emitted from this product.
Do not look at operating lamp. Avoid eye
exposure. Use appropriate shielding or
eye protection.
IR
AVERTISSEMENT Ce produit émet un
rayonnement infrarouge. Ne regardez pas
la lampe lorsqu‘elle est allumée.
Évitez tout exposition aux yeux.
Utilisez un écran ou dispositif de
protection oculaire approprié.
RISK GROUP 3
Groupe de risque 3
9
3. Safety Notes
Applicable for LED1/3/5/8
RISK GROUP 3
WARNING UV emitted from this product.
Avoid eye and skin exposure to
unshielded product
UV
Groupe de risque 3
AVERTISSEMENT Ce produit émet un
rayonnement ultraviolet. Évitez d‘exposer
les yeux et la peau à un produit sans
protection.
The Leica LED1, LED3, LED5 and LED8 compact light sources generate high-energy light
with invisible UV components and has been
classified in risk group 3 (high risk) in accordance with EN62471-1 while the light guide
is not connected to the microscope/system.
There is a general risk of being dazzled or
blinded by light! Never look directly into the
light guide output of the instrument or into
the output of the light guide connected to the
instrument. Always ensure that the output of
the connected light guide is securely connected to the system to be illuminated before
switching on the compact light source. Furthermore, before switching on the compact
light source, dim the light output to prevent
damage to the connected system. During operation, the light guide must not be removed
from the microscope/system. While connected to the microscope/system, the risk group 1
(low risk) has been classified. Please see the
instructions on this topic in the microscope
instructions for use.
Warning: Possibly hazardous optical radiation
emitted from this product. Do not look at operating lamp. Eye injury may result.
Warning: Infrared (IR) emitted from this product.
Do not look at operating lamp.
Warning: UV emitted from this product. Avoid
eye and skin exposure to unshielded product.
Caution: DO NOT open the unit. There are no
serviceable parts inside and opening the light
engine enclosure will void the manufacturer’s
warranty.
Caution: DO NOT set liquids on the light engine.
Spilled liquids may damage your light engine.
Caution: DO NOT drop the light engine. It contains glass optical components that could be
damaged or misaligned by the shock produced
by a drop onto a hard surface.
DISCLAIMER: Leica shall not be liable for injury to
the user or damage to the product resulting from
the light source being used in a way for which it
was not intended and in complete disregard for
all posted safety precautions and warnings.
10
Attention!
The instruments and accessories described
in this Instructions for use have been tested
for safety and potential hazards.
The responsible Leica representative or the
main plant in Wetzlar must be consulted
whenever the instrument is altered, modified
or used in conjunction with non-Leica components that are outside of the scope of this
manual!
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims!
Attention!
If any safety defects or malfunctions of the
compact light sources are identified, the
instrument must be immediately taken out
of operation, disconnected from the power
system at all poles and secured against further use. For repair, the compact light source
must be sent to the vendor or an authorized
representative of the vendor.
3. Safety Notes
11