Leica AutoStainer XL Instruction manual
Instruction Manual
Leica AutoStainerXL, V2.1 English – 10/2002
Always keep this manual near the instrument!
Read carefully prior to operating the instrument!
Leica
AutoStainer XL
Automated slide stainer
NOTE
The information, numerical data, notes and value
judgments contained in this manual represent the
current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field.
We are under no obligation to update the present
manual according to the latest technical developments, nor to provide our customers with additional copies, updates etc. of this manual.
For erroneous statements, drawings, technical
illustrations etc. contained in this manual we exclude liability as far as permissible according to
the national legal system applicable in each individual case. In particular, no liability whatsoever
is accepted for any financial loss or consequential damage caused by or related to compliance
with statements or other information in this
manual.
Statements, drawings, illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our
products.
These are determined only by the contract provisions agreed between ourselves and our customers.
Leica reserves the right to change technical
specifications as well as manufacturing processes without prior notice. Only in this way is it
possible to continuously improve the technology
and manufacturing techniques used in our products.
This document is protected under copyright laws.
Any copyrights of this document are retained by
Leica Microsystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including any electronic systems and media – requires
express prior permission in writing by Leica
Microsystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Microsystems Nussloch GmbH
Published by:
Leica Microsystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: +49 (0)6224 143-0
Fax: +49 (0)6224 143-200
eMail: histo_info@leica-microsystems.com
Internet: http://www.histo-solutions.com
Leica AutoStainer XL – Automated slide stainer
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Table of contents
1. Important information .................................................................................................... 6
1.1 Symbols occurring in the text and ............................................................................................6
1.2 Designated use / improper use of the instrument .................................................................. 7
1.3 Qualification of personnel.......................................................................................................... 7
2. Safety................................................................................................................................. 8
2.1 Safety regulations ....................................................................................................................... 8
3. Instrument features ........................................................................................................ 9
3.1 Overview – instrument.............................................................................................................. 10
3.2 Standard delivery - packing list .............................................................................................. 12
3.3 Technical Data........................................................................................................................... 13
4. Installation ..................................................................................................................... 14
4.1 Site requirements ...................................................................................................................... 14
4.2 Connection.................................................................................................................................. 14
4.2.1 Power .......................................................................................................................................... 14
4.2.2 Water supply .............................................................................................................................. 15
4.3 Battery backup - UPS (optional)..............................................................................................16
4.4 Remote alarm (optional) ........................................................................................................... 17
4.5 Fume control system................................................................................................................. 17
4.6 Oven............................................................................................................................................. 17
5. Operation ........................................................................................................................ 18
5.1 The control panel....................................................................................................................... 19
5.2 The main menu........................................................................................................................... 20
5.3 Menu map................................................................................................................................... 21
5.4 Edit a program ............................................................................................................................ 22
5.4.1 Entering steps ............................................................................................................................ 23
5.4.2 Erasing steps.............................................................................................................................. 24
5.4.3 Inserting a blank step into a program .................................................................................... 24
5.4.4 Removing blank steps in a program ....................................................................................... 25
5.4.5 Saving a program ...................................................................................................................... 25
5.4.6 Deleting a program.................................................................................................................... 25
5.4.7 Copying a program .................................................................................................................... 26
5.4.8 Viewing a program .................................................................................................................... 27
5.4.9 Checking program compatibility .............................................................................................27
5.5 User-adjustable parameters.................................................................................................... 29
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Instruction manual V2.1 – 10/2002
Table of contents
5.6 Oven ............................................................................................................................................. 29
5.7 Agitation (Dips) .......................................................................................................................... 30
5.8 Rack movement times............................................................................................................... 31
5.9 Staining ....................................................................................................................................... 31
5.9.1 Reagent containers................................................................................................................... 32
5.9.2 Wash system.............................................................................................................................. 32
5.9.3 Water saving .............................................................................................................................. 33
5.9.4 Loading slide racks ................................................................................................................... 33
5.9.5 Unloading racks from the exit drawer.................................................................................... 34
5.9.6 Unloading racks from other stations...................................................................................... 34
5.9.7 Interrupting staining.................................................................................................................. 35
5.9.8 Aborting a rack .......................................................................................................................... 36
6. Cleaning and Maintenance ........................................................................................ 37
6.1 Cleaning the instrument ........................................................................................................... 37
6.1.1 Wash containers ....................................................................................................................... 37
6.1.2 Reagent containers................................................................................................................... 37
6.1.3 Slide racks .................................................................................................................................. 37
6.1.4 Oven............................................................................................................................................. 37
7. Trouble shooting ........................................................................................................... 38
7.1 Instrument failures .................................................................................................................... 39
7.2 Information and warnings ........................................................................................................ 40
7.2.1 During staining........................................................................................................................... 40
7.2.2 During editing programs........................................................................................................... 41
7.2.3 During SetUp .............................................................................................................................. 42
8. Warranty and service .................................................................................................. 43
APPENDIX 1 ................................................................................................................... 44
User-adjustable parameters ............................................................................................................... 44
APPENDIX 2 ................................................................................................................... 45
Consumables and accessories........................................................................................................... 45
APPENDIX 3 ................................................................................................................... 46
Compatible staining programs ............................................................................................................ 46
Glossary .................................................................................................................................... 48
Leica AutoStainer XL – Automated slide stainer
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1. Important information
The instruction manual for the Leica
AutoStainer XL includes chapters dealing
with the following subjects:
Chapter 1 Manual structure including:
• Table of contents
• Important information
on this handbook.
Chapter 2 Safety
• Read this chapter before you
attempt to operate the
instrument!
Chapter 3 Instrument features
• General description
• Technical data
Chapter 4 Installation
• Site requirements
• Installation
Chapter 5 Operation
• Control elements
• Menu map
Chapter 6 Trouble shooting
Chapter 7 Cleaning and maintenance
Chapter 8 Warranty and service
Appendix 1 User-adjustable parameters
Appendix 2 Consumables
and accessories
1.1 Symbols occurring in the text and their meaning
Warnings and cautions appear in a
grey box and are marked by a
warning triangle
Notes,
i. e. important information for the
user appear in a grey box and are
marked by an information sign
.
(5)
(Fig. 5)
Instrument type:
All information in this instruction manual applies
only to the instrument type indicated on the title
page.
A nameplate with the instrument serial number is
fixed on the back of the instrument.
Required information:
For all inquiries it is important to state the following:
Numbers in parentheses refer to
positions in the illustrations or to
the illustrations themselves.
.
Appendix 3 Compatible staining programs
Glossary
6
• instrument type
• serial number.
Instruction manual V 2.1 – 10/2002
General
This instruction manual includes important information related to the operating safety and maintenance of the instrument.
The instruction manual is an important part of the product. It must be read
carefully and completely before setup and first use of the instrument and
must be kept near the instrument at all times.
If additional requirements on accident prevention and environmental protection exist in the country of operation, this instruction manual must be
supplemented by appropriate instructions to ensure compliance with such
requirements.
Be sure to read and comply with the safety instructions,
warnings and cautions in chapter 2, even if you are already
familiar with the operation and use of other Leica products.
1.2 Designated use / improper use of the instrument
- The AutoStainer XL has been designed for staining applications in medicine, biology and industry.
- The instrument may be operated only according to the instructions contained in this manual.
- Any other use is considered an improper use of the product.
1. Important information
1.3 Qualification of personnel
- The AutoStainer XL may be operated only by trained laboratory personnel.
- All laboratory personnel designated to operate the AutoStainer XL must
read this instruction manual carefully and must be familiar with all technical features of the instrument before attempting to operate the
AutoStainer XL.
Leica AutoStainer XL – Automated slide stainer
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2. Safety
2.1 Safety regulations
This instrument was built and tested in accordance with the safety regulations for electrical measuring, control, regulating and laboratory devices
specified below
• DIN EN 292,
• DIN EN 61010-1,
• EN 50082-1,
• EN 55011,
• IEC 1000-4
as well as in accordance with the international quality standard
• DIN ISO 9001.
In order to maintain this condition and to ensure safe operation, the operator must observe the instructions and warnings contained in this instruction
manual.
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Instruction manual V 2.1 – 10/2002
3. Instrument features
The AutoStainer XL is the result of an extensive research program to provide an innovative stainer which meets the quality requirements of the modern laboratory, as well as:
• high throughput,
• flexibility,
• safety.
The AutoStainer XL achieves its high throughput by means of an innovative
slide rack transfer mechanism which allows continuous loading of up 11
racks of 30 slides each.
The flexibility of AutoStainer XL also permits simultaneous processing of
slide racks according to different staining protocols so that Papanicolau
and Hematoxylin/Eosin staining can be conveniently performed at the same
time, without reprogramming or reagent changes.
The AutoStainer XL incorporates all of the features which ensure high flexibility, convenience and above all, quality staining. A fan-forced oven is available to quickly dry slides and optimized wash stations result in rapid removal of excess reagent. The minimum-carryover design of the slide racks
ensures that there are no drips and reagent life is extended.
The AutoStainer XL is safe to use and has an integral fume control system.
Rack loading and unloading is achieved by a unique two-drawer system
which means virtually no exposure to fumes.
The outstanding flexibility, throughput and quality-staining capability of
AutoStainer XL has set a new standard in staining excellence.
Leica AutoStainer XL – Automated slide stainer
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3. Instrument features
3.1 Overview – instrument
20
1
2
16
15
Abb. 1
19
18
17
11
14
13
10
12
3
4
5
6
7
8
9
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Instruction manual V2.1 – 10/2002
3. Instrument features
21
30
Abb. 2
27 26 25 28
35
Front view
1. Transfer mechanism
2. Wash stations
3. Oven
4. Program pad
5. Program pad recess
6. ON/STOP switch
7. Display screen
8. Keypad
9. LED indicators
10. Slide rack
11. Lid
12. Reagent container
13. Load drawer
14. Load LED and key
15. Exit drawer
16. Slotted lid
17. Exit LED and key
18. Container map on fume filter cover
19. Lid support
20. Lid
24
23
3231
34
33
22
Rear panel
21. Water inlet
22. Drain outlet
23. Serial port
24. Power supply
25. Oven voltage selector
26. Mains switch (ON/OFF)
27. Mains outlet
28. Feet, adjustable
29. Power jumper cable
30. Power supply inlet
31. Rating and serial number plate
32. Remote alarm socket, 50 V 1 A max
33. Accessory port
34. Exhaust air duct
35. Power supply outlet
Leica AutoStainer XL – Automated slide stainer
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3. Instrument features
3.2 Standard delivery - packing list
Standard delivery:
1 Leica ST5010 basic instrument (100-120 V/50-60 Hz)
1 accessories kit (0456 35660) consisting of:
- 22 reagent vessels with lids..................................................................................................... 0475 33659
- 5 wash vessels........................................................................................................................... 0456 35268
- 5 slide racks, metal.................................................................................................................... 0456 33919
- 2 lids with slots for reagent vessels ....................................................................................... 0475 34486
- 1 jumper cable - supply system............................................................................................... 0411 34604
- 1 remote alarm connector........................................................................................................ 6844 01005
- 1 hose clamp .............................................................................................................................. 0422 31972
- 1 angular connecting nozzle for hose .................................................................................... 0475 33669
- 1 V filter 3/4 ................................................................................................................................. 0456 36101
1 activated carbon filter................................................................................................................. 0474 32273
1 filter cover ..................................................................................................................................... 0456 35240
1 drip tray for paraffin (heating) (inside the instrument)........................................................... 0456 35216
1 dye log block (attached to the instrument) .............................................................................. 0456 35459
1 water supply hose with seal ...................................................................................................... 0474 32325
1 drain hose ..................................................................................................................................... 0475 35748
1 power cable USA-C-J ................................................................................................................. 0411 13559
1 operating manual Leica AutostainerXL G/E/F/S .................................................................... 0456 25634
- 1 reference manual as appendix, English only (dye logs, etc.)
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Instruction manual V2.1 – 10/2002
3. Instrument features
3.3 Technical Data
Specimen slide throughput: .............................................................. at least 200 specimen slides per hour
(depending on the selected program - up to 600 slides per hour)
Loading capacity: ......................................................................................................................... 11 slide racks
Slide rack capacity:............................................................................................................. 30 specimen slides
Total number of stations:................................................................................................................................. 26
Total number of reagent stations:.................................................................................................... at least 18
Reagent container volume: ...................................................................................................................... 450 ml
Number of wash stations: ........................................................................................................................ max. 5
Oven: ..................................................................................................................................................................... 1
Oven chamber temperature:.................................................................................... ambient or 30°C to 65°C
Incubation time setting: ............................................................................... from 0 sec. up to 99 min., 59 sec
Load/unload stations:............................................................................................................................... 1 each
Permanent memory capacity:.................................................. 15 programs, up to 25 program steps each
Operating temperature range:.................................................................................................... 15 °C to 35 °C
Relative humidity:................................................................................................. max. 80 %, non-condensing
Dimensions (W x D x H): ............................................................................................. 109 cm x 67 cm x 51 cm
Weight: .......................................................................................................................................................... 65 kg
Voltages: .................................................................................................................. 110 V - 120 V, 50 Hz - 60 Hz
230 V - 240 V, 50 Hz - 60 Hz
Leica AutoStainer XL – Automated slide stainer
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4. Installation
4.1 Site requirements
Instructions on how to install the instrument are provided in this chapter. A
diagram and description of components is also given. Finally, the procedure
for replacement of the fume filter is outlined.
The AutoStainer XL requires a solid bench of dimensions 1.090 mm long and
670 mm deep. The instrument must be located within 3 meters of a tap and
drain.
The power requirements for the AutoStainer XL are:
8 amps: at 110 volts
4 amps: at 240 volts
The voltage selector and other internal components are set by the manufacturer to suit the country of sale.
The voltage selector setting must not be altered by the user.
4.2 Connection
4.2.1 Power
14
The AutoStainer XL requires connection to a laboratory water tap with mains
pressure fitting.
• Connect the power cord to the mains outlet ((28) page 11).
• Connect the power jumper cable to the power supply outlet ((30) page
11) and to the power supply inlet ((32) page 11).
Refer to Figure 10 on page 11.
Instruction manual V2.1 – 10/2002
4. Installation
4.2.2 Water supply
How to switch on:
1. Connect the power cord to the mains power socket and, if applicable,
switch power ON at the mains socket.
2. Set the ON/STOP switch at the side of the unit to STOP.
3. Set the ON/OFF switch at the rear of the unit to ON.
4. Set the ON/STOP switch to ON.
The instrument will then sound 3 short beeps and the Main Menu will be
displayed.
When the instrument is not in use set the ON/STOP switch at the side to
STOP.
The ON/OFF switch at the rear of the instrument should be left ON.
The instrument must never be operated without the power
jumper cable.
The instrument must be connected to an earthed mains
power outlet socket only.
Connect the water hose to the water inlet at the rear of the unit. Screw the
other end of the hose to the cold water tap. The hose has a 3/4 inch BSP
fitting. Slowly turn the tap on fully.
Ensure that the water filter is present when fitting the water
inlet hose. Failure to do so may result in leakage of water.
Drain hose
Connect the drain hose to the drain outlet on the rear of the unit.
Leica AutoStainer XL – Automated slide stainer
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