Leica APPLICATION SUITE POWERMOSAIC User Manual

LAS Power

Mosaic

Get the Big Picture

LAS Power Mosaic is designed to bring a whole
new dimension to microscopy, providing you
with a unique understanding of the relationship
between microscopic features and the overall
structure of the specimen.

LAS Power Mosaic provides the ultimate in
high-resolution specimen visualisation. Scan
the entire specimen or select a specific region
of interest region and it’s scanned at high
speed. The captured images are immediately
combined to form a seamless mosaic image.

Once the scan is complete you can relocate
effortlessly to areas of interest with a single
mouse click and can view the live microscope
image that corresponds to a chosen location.
Additionally you can zoom and pan around the
entire digitised mosaic image using easy and
intuitive “browsing” tools.

The acquired images, including the entire
mosaic can be saved for subsequent review,
discussion with colleagues or publication.

LAS Power Mosaic is ideal for both routine
and research applications and provides
modes of scanning to suit all forms of optical
microscopy. It’s specifically developed to be
both versatile and easy to use. This powerful
LAS option provides the microscopist with a
high performance mosaic imaging solution
without compromising conventional use of the
microscope.

Key benefits include:

�

Scanning and acquisition at camera
frame rates to ensure high speed and
rapid throughput

�

Tile edge merging provides highest
possible image quality

�

No restrictions on objective or imaging
method

�

Fast and accurate relocation with easy
and intuitive review tools

�

Intelligent memory management
supports mosaic sizes limited only by
available disk space

�

Fully automated focus control and
tracking

�

User control of image size to ensure
suitability for publication and archiving.

�

One click calibration for easy camera
alignment

�

Optional advanced functions, including
powerful 3-D Z-Stack mosaic acquisition
for powerful focus visualisation

Z-Stack Mosaic
In addition to the standard scanning and review
capabilities, the Power Mosaic Plus module
further extends the capability using “Z-Stack”
acquisition to provide 3-D mosaic imaging and
visualisation, ideally suited to specimens with a
wide focal range.

Features at a Glance

Power Mosaic Scanning

�

Uses triggered image capture for fast
continuous scan and acquire

�

Standard scan available using step and
acquire for low light applications

�

Image streaming for mosaic sizes only
limited by disk space

�

Additional scans can be easily added to
extend an initial scan

Scan Patterns

�

Rectangle, Circular, Annular, Cross (+ and x),
or Random

�

Overlap of tiles allows joins to be merged
smoothly

�

Camera rotation is automatically corrected

�

Create a scan pattern interactively or by
entering the exact details

Microscope Automation

�

An Oasis XY stage and Z focus control drive
board is used

�

A software joystick or Leica smart move can
be used for stage and focus movement

�

Fully compatible with Leica Microsystems
LAS configured microscopes handling focus,
turret, condenser and lamp controls as
available

Leica DFC Camera

�

Exposure, saturation, gain and gamma
control from LAS controls

�

Triggered acquisition from progressive scan
and DFC FX cameras for fastest scans

�

Automatic and manual white balance

�

Color or monochrome acquisition (8 or 16-bit)

�

Shading correction for smooth mosaic
results