Integra CELLROLL, CELLSPIN Operating Instructions Manual
CELLROLL and CELLSPIN
Operating instructions
183411_V12
2
Declaration of Conformity | Konformitätserklärung | Déclaration de conformité
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the products |
erklärt in alleiniger Verantwortung, dass die Produkte |
déclare sous sa responsabilité exclusive, que les produits
in accordance with the EC directives | gemäß der EU-Richtlinien |
sont conforme au terme de la directives CE
are in compliance with the following standards or normative documents: |
mit den folgenden normativen Dokumenten übereinstimmen: |
aux documents normatifs ci-après :
Standards for Canada and USA
Zizers, December 9th 2013
ledom ,epyTnoitpircseD
500681 ,100681LLORLLEC
100381NIPSLLEC
2006/95/EC Low voltage equipment
2004/108/EC Electromagnetic compatibility
2011/65/EC Restriction of Hazardous Substances
2002/96/EC Waste Electrical and Electronic Equipment
EN 61010-1 Safety requirements for electrical equipment
for measurement, control and laboratory use,
Part 1: General requirements.
EN 61326-1 Electrical equipment for measurement, control
and laboratory use - EMC requirements.
CAN/CSA-C22.2 No. 61010-1 Safety requirements for electrical equipment
for measurement, control and laboratory use,
Part 1: General requirements.
UL Std. No. 61010-1 Safety requirements for electrical equipment
for measurement, control and laboratory use,
Part 1: General requirements.
FCC, Part 15, Class A Emission
Elmar Morscher
CEO
Thomas Neher
Quality Manager
2.1 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Contents
1. Safety precautions
2. Technical data
4. Assembly and installation CELLROLL/CELLSPIN
3. Abridged operating instructions CELLROLL/CELLSPIN
3.1 Instrument parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.2 Description of keys and functions . . . . . . . . . . . . . . . . . . . . . 10
3.3 Abridged instructions CELLROLL . . . . . . . . . . . . . . . . . . . . . 11
3.4 Abridged instructions CELLSPIN . . . . . . . . . . . . . . . . . . . . . . 13
4.1 Equipment provided - CELLROLL . . . . . . . . . . . . . . . . . . . . . 15
4.2 Equipment provided - CELLSPIN . . . . . . . . . . . . . . . . . . . . . . 16
4.3 Control unit CELLROLL/CELLSPIN . . . . . . . . . . . . . . . . . . . . 16
4.4 Designation of individual instrument parts . . . . . . . . . . . . . . 17
4.5 Vertical assembly - CELLROLL . . . . . . . . . . . . . . . . . . . . . . . 18
4.6 Horizontal assembly - CELLROLL . . . . . . . . . . . . . . . . . . . . . 19
4.7 Disassembly - CELLROLL . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.8 Stirring unit - CELLSPIN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.9 Connecting the printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.10 Equipment disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
General precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Installation of CELLROLL/CELLSPIN: location and conditions . . . . . . . . . . . . 5
Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Conditions of use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Dangers involved when safety precautions are not observed . . . . . . . . . . . . . 6
Safety at work . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Qualification of operating personnel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Safety aspects for user company . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Changes in configuration/design by user . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Power requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3
4
5.1 Operating steps
Starting and stopping the system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Switching on and off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Selecting outputs (OUTPUT 1/OUTPUT 2) . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Configuration of connected instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.2 CELLSPIN - specific CELLSPIN functions
Stirring - "SPEED" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Pendular stirring angle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Interval mode "––" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5.3 CELLROLL - specific CELLROLL functions
Rolling speed - "SPEED" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Rocking angle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.4 CELLROLL/CELLSPIN - common functions
Running time mode "" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
Cycle mode "CYCLES" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Printout and overview of program parameters "PRINT . . . . . . . . . . . . . . . . . . . 30
Storing programs "STORE" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Recalling and viewing programs "RECALL" . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
6. Troubleshooting
7. Guarantee/Cleaning
5. Detailed operating instructions
8. Manufacturer and Service Contacts
5
General
Throughout the manual, the following symbols will be used to indicate general precautionary measures:
Indication: information on the correct use of
CELLROLL/CELLSPIN.
Warning: Warning that, if safety precautions are not
observed, damage may result to the instruments.
Please read all documentation.
Danger: Warning that, if safety precautions are not
observed, injury to personnel may result.
Installation: Location and conditions
CELLROLL/CELLSPIN are intended for use in a laboratory.
Ambient conditions for the devices CELLROLL/
CELLSPIN see chapter Technical data
Warning: Always locate the control unit outside of an incubator if used.
Avoid exposing the control unit and power supply to direct
sunlight or high humidity.
Locate CELLROLL in such a way that personnel
cannot come in contact with the cogs.
Transport
Always grip CELLROLL on the base when transporting
1. Safety precautions
6
Conditions of use
CELLROLL is a modular and expandable roller system for cell cul-
tivation and preparation of cell products in roller bottles. It is suitable for use with adherent cells and cell suspensions. The instrument is not designed for any other applications and should therefore not be used for such.
CELLSPIN is a spinning system comprising four stirring units for
the gentle cultivation of cells and the preparation of cell products in
spinning vessels. It is suitable for use with cell suspensions and immobilised adherent cells (e.g. microcarriers/alginate inclusion). The instrument is not designed for any other applications and should therefore not be used for such.
Dangers involved when safety precautions are not
observed
CELLROLL/CELLSPIN are state-of-the-art instruments with respect
to current technology and are safe to use. Some danger may exist
if the instruments are used by untrained personnel. Any person given
the task of operating CELLROLL/CELLSPIN must first read the operating and safety instructions and understand them fully or be thoroughly trained by a superior in the use of the instruments before
operating the instrument.
Safety at work
Independent of any safety precautions listed here, all other relevant
safety aspects - e.g. GLP, GMP, professional trade associations,
Department of Health, trade supervisory authorities - must be
observed.
Qualification of operating personnel
Normally, a technical qualification is required. Specialist knowledge
is required when using CELLROLL/CELLSPIN if all danger is to be
avoided.
Personnel without formal technical qualifications do not as a rule
possess such specialised information and should therefore only be
allowed to operate CELLROLL/CELLSPIN if properly trained and
under the supervision of suitably qualified personnel.
Safety aspects for the user company
The supervisors of operating personnel must have read and understood all the safety precautions involved and must ensure that these
are adhered to. They must also ensure that operating personnel also
understand and observe all safety rules.
Prior to operating CELLROLL/CELLSPIN, the supervisor must ensure
that no other dangers exist, especially if other instruments and systems are also being used.
If necessary, additional safety instructions must be compiled by the
supervisor. The user company must, if necessary, in order to exclude
all possible danger, compile explicit safety instructions and have these
signed by the staff responsible. In addition, clear competences must
be established as to who is responsible for the operation of CELLROLL/CELLSPIN so that these potential dangers can be reduced
to an absolute minimum.
7
Changes in configuration/design by user
No changes may be made to CELLROLL/CELLSPIN. Any parts that
become defective must be replaced by original INTEGRA Biosciences
parts.
CELLROLL/CELLSPIN may not be altered in design or in any of their
safety aspects without the express written permission of INTEGRA
Biosciences. In particular, no alterations may be made to any of the
protective functions. Any alteration made releases INTEGRA
Biosciences from any liability for damage caused.
Power requirements
Checking the power supply
Check the power supply at the location and ensure that it complies
with that of the instrument.
Indication: Using the wrong power supply may damage
electronic components in the instrument.
Connecting to the power supply
Connect CELLROLL/CELLSPIN to the power supply using the cable
provid ed.
Danger: The instrument may not be used if insufficient
protection is provided as this may expose personnel
to the danger of electrical current injury.
NB: When connecting or disconnecting the instrument
from the control unit the latter must always first be
switched off otherwise the electronics may be damaged or a program interrupted.
8
2. Technical data
CELLROLL
Width Depth Height
1 Deck 295 mm 360 mm 240 mm
2 Decks 295 mm 360 mm 400 mm
3 Decks 295 mm 360 mm 560 mm
4 Decks 295 mm 360 mm 720 mm
Control unit: 295 mm 80 mm 60 mm
Speed: "Motor unit": 0.1 – 2.0 rpm
"Motor unit fast": 2.0 – 6.0 rpm
Power Supply:
Input: 100 – 240 VAC/47 – 63 Hz/1.6 A
Output: 9.5 VDC/4.0 A/38 W
Weight:
Motor unit: 1.85 kg
Deck: 1.7 kg
Operating Condition:
Operation
Motor-unit/ Deck: 5 – 40 °C/max. 99% RH/max. 10% CO
2
Operation Control unit: 5 – 40 °C/max. 80% RH
Storage: -10 – 50 °C/max. 95% RH
Degree of protection:
Motor-unit/Deck: IP54
Control unit: IP21
2.1 Technical data
CELLSPIN
Width Depth Height
Drive: 295 mm 330 mm 60 mm
Control unit: 295 mm 80 mm 60 mm
Speed: 5 – 75 rpm
Power Supply:
Input: 100 – 240 VAC/47 – 63 Hz/1.6 A
Output: 9.5 VDC/4.0 A/38 W
Weight:
Control unit: 0.5 kg
Stirrer unit: 2.9 kg
Operating Condition:
Operation Stirrer unit: 5 – 40 °C/max. 99% RH/max. 10% CO
2
Operation Control unit: 5 – 40 °C/max. 80% RH
Storage: -10 – 50 °C/max. 95% RH
Degree of protection:
Stirrer unit: IP54
Control unit: IP21
9
3.1 Instrument parameters
3. Abridged operating instructions
CELLROLL /CELLSPIN
Storing of programs – "STORE"
Memories Pr. 01 – Pr. 10
Recalling programs – "RECALL"
Pr. 01 – Pr. 10
CELLROLL
Motor unit fast (2 – 6 rpm)
"SPEED" for continuous rolling
(rolling with no directional change)
2.0 – 6.0 in steps of 0.25
and for rocking
(rolling with directional change)
ANGLE " " for rocking
(180° – 1440° in steps of 180°)
ANGLE ""for rolling = 0°
CELLSPIN
"SPEED" for continuous stirring
(= continuous stirring)
5.0 – 40.0 rpm in steps of 2.5
40.0 – 75.0 rpm in steps of 5.0
"SPEED" for pendular stirring
(= stirring with directional change)
5.0 – 40.0 rpm in steps of 2.5
ANGLE " " for pendular stirring
(180° – 1440° in steps of 180°)
ANGLE ""continuous stirring = 0°
RUNNING TIME ""
(00.05 h.min. - 99.59 h.min.) or
unlimited = Con
Cycles – "CYCLES"
for programming
1 – 9999
CELLROLL
Motor unit (0.1 – 2 rpm)
"SPEED" for continuous rolling
(rolling with no directional change)
0.1 – 1.0 in steps of 0.1
1.0 – 2.0 in steps of 0.5
and for rocking
(rolling with directional change)
ANGLE " " for rocking
(180° – 1440° in steps of 180°)
ANGLE ""for rolling = 0°
Print-out of protocols – "PRINT"
For viewing stored programs P1
Protocol print-out P2
Print-out of stored programs P3
Storing of programs – "STORE"
Memories Pr. 01 – Pr. 10
Recalling programs – "RECALL"
Pr. 01 – Pr. 10
RUNNING TIME ""
(00.05 h.min. - 99.59 h.min.) or
unlimited = Con
PAUSE " "
(00.00 h.min. - 99.59 h.min.)
Cycles – "CYCLES"
for programming
1 – 9999
Print-out of protocols – "PRINT"
For viewing stored programs P1
Protocol print-out P2
Print-out of stored programs P3
3.2 Brief description of keys and functions
Keys:
ON/OFF: Switches the instrument on/off
SELECT: Switches between parameters. The corresponding green LED will light and the most
recently set value will appear in the display.
ENTER: For confirming the value entered.
∨/∧: Keys "∨" and "∧" alter the corresponding parameters stepwise, the values being shown on
the display.
By keeping keys "∨" and "∧" pressed, all parameter values are displayed in rapid sequence.
< >: For:
a) Changing e.g. from hours to minutes and vice versa when setting time.
b) Switching between OUTPUT 1 and OUTPUT 2 in the functions "SPEED" and "RECALL".
c) Activating/deactivating the print options P2 and P3 in the function "PRINT".
Function LED’s :
STORE: Storing of programs
PRINT: P1: Display of stored programs
P2: Setting the printing interval
P3: Print out of stored programs
RECALL: Recalling stored programs
PUMP: Additional function
CYCLES: Number of times a program should be repeated
: Angle for pendular/circular stirring and rocking/rolling
ALARM: Red lamp lights if there is malfunction
SPEED: Speeds for pendular/circular stirring and rocking/rolling and for selecting between
OUTPUT 1 and OUTPUT 2
–––– : Pause between two programmed runs
: Running time (specific/continuous) for pendular or continuous stirring, rocking or rolling
h/min: Activated during entry of time parameter
rpm: Activated during entry of speed
2
4
3
4
1
17
12
5
16
11
6
13
7
15
8
14
9
17
16
10
10
11
7
1213
6
8
14
9
10
2
3
5
15
1
NB: The control unit must always be located outside the incubator!
– Connect C ELLRO LL t o the control u nit with the cable
provided.
If connection has not been made, "Err 02" will appear on the
dis play on starting the instrument. Check the connection and
cancel the error message by pressing "ENTER".
– In the event of a power failure, any unstored data of both out-
puts will be automatically stored and recalled once the power has
been restored. The "Err 03" in the display can be cancelled by
pressing "ENTER".
Entering the program for working with CELLROLL
☞ Switch on the instrument by pressing the ON/OFF key. The
green LED under "RECALL" will light.
☞ Press "SELECT" and select the function "SPEED".
In the function "SPEED":
Selecting the output:
☞ Select OUTPUT 1 or OUTPUT 2 via key "< >".
Setting the rolling speed:
CELLROLL is equipped with two different motors. The control unit
will always select the right one.
"CELLROLL" Motor unit (0.1-2.0 rpm):
☞ Set the speed within the range 0.1 - 2.0 rpm via "∨" and "∧".
Confirm by pressing "ENTER".
"CELLROLL" Motor unit fast (2.0-6.0 rpm):
☞ Set the speed within the range 2.0 - 6.0 rpm via "∨" and "∧".
Confirm by pressing "ENTER".
In the function " ":
Selecting the pendular angle:
☞ For continuous rolling (no directional change), set the
pendulum angle to "0°" via "∨". Confirm by pressing "ENTER".
☞ For rocking (with directional change), set the pendulum angle
within the range 180° - 1440° (= 0.5 - 4.0 bottle revolutions).
Confirm by pressing "ENTER".
NB: If "rocking" has been selected, please observe the restrictions
impos ed below under " " and "–".
In the function "":
Set the rolling duration:
The duration of continuous rolling or rocking can be set to:
- A limited time between 00.05 (h/min) - 99.59 (h/min), or
- An unlimited time as "Con".
☞ To select a specific rolling duration
, set hours and/or minutes
via the "< >" key and alter via "∨" and "∧". Confirm by pressing
"ENTER".
☞ To select continuous rolling or rocking operation, activate the
h/min function and press the "∨" key until the display shows
less than 0.05 and "Con" appears. Confirm by pressing
"ENTER".
11
3.3 Abridged operating instructions - CELLROLL
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