Integra ASSIST PLUS Operating Instructions Manual
ASSIST PLUS Operating instructions
128950_V02
Declaration of conformity
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the ASSIST PLUS (Model 4505) complies with:
EU Directives
Low Voltage Equipment 2014/35/EU
Electromagnetic Compatibility 2014/30/EU
Restriction of Hazardous Substances 2011/65/EU
Waste Electrical and Electronic Equipment 2012/19/EU
Radio Equipment Directive 2014/53/EU
EU Regulations
Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH)
Ecodesign - Power supplies 278/2009
Standards for EU (titles shortened)
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
Particular requirements for automatic and semi-automatic
laboratory equipment for analysis and other purposes.
Electrical equipment for measurement, control and laboratory
use - EMC requirements.
Radio equipment and services - Common requirements EN 301 489-1 V2.2.0
Specific conditions for Broadband Data Transmission Systems EN 301 489-17 V3.2.0
Wideband transmission systems - Access to radio spectrum EN 300 328 V2.2
EN 61010-1: 2010
EN 61010-2-81: 2015
EN 61326-1: 2013
1907/2006
Standards for Canada and USA
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
Particular requirements for automatic and semi-automatic
laboratory equipment for analysis and other purposes.
Operation is subject to the following two conditions:
(1) this device may not cause harmful interference, and
(2) this device must accept any interference received, including
interference that may cause undesired operation.
Zizers, October 7, 2019
Urs Hartmann
CEO
Thomas Neher
Quality Manager
CAN/CSA-C22.2
No. 61010-1
UL 61010-1
UL 61010-2-81
Part 15 of the
FCC Rules
Contains FCC ID:
PI4410B
ASSIST PLUS – Operating instructions V02
Table of Contents
Chapter 1 Introduction
1.1 Symbols used...................................................................................5
1.2 Intended use.....................................................................................6
1.3 Safety notes .....................................................................................7
Chapter 2 Description of the device
2.1 Scope of delivery..............................................................................8
2.2 Overview of the ASSIST PLUS ........................................................8
2.2.1 ASSIST PLUS base unit .......................................................8
2.2.2 Touch panel ..........................................................................9
2.2.3 Interfaces ..............................................................................9
2.3 Overview of INTEGRA Electronic Pipettes.....................................10
2.3.1 Pipette parts........................................................................10
2.3.2 Display ................................................................................10
Chapter 3 Installation
3.1 Operating environment...................................................................11
3.2 Setting up and moving of the instrument........................................11
3.3 Charging the battery of the electronic pipette.................................11
3.4 Removing the tip ejector lid ............................................................12
3.5 Adapting the pipette holder ............................................................12
3.6 Attaching and removing an electronic pipette ................................12
3.7 Inserting decks ...............................................................................13
3.8 Inserting waste bags and waste bin ...............................................13
3.9 Toolbox settings .............................................................................14
3.9.1 Enabling ASSIST PLUS program .......................................14
3.9.2 Overview of Toolbox for ASSIST PLUS..............................14
3.9.3 Device Information..............................................................14
3.9.4 Bluetooth Pairing.................................................................15
3.9.5 Tip Monitor ..........................................................................15
3.9.6 Setting tip type ....................................................................15
3.9.7 Position adjustment.............................................................16
Chapter 4 Operation
4.1 Turn on/off the ASSIST PLUS device ............................................17
4.2 Turn on/off an INTEGRA electronic pipette....................................17
4.3 Connection ASSIST PLUS to Pipette.............................................17
4.3.1 Bluetooth connection ..........................................................17
4.3.2 Via communication cable ....................................................18
4.4 Running a program.........................................................................18
4.5 Troubleshooting..............................................................................19
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ASSIST PLUS – Operating instructions V02
Chapter 5 Programming
5.1 Overview pipetting programs..........................................................20
5.2 Setting up a program directly on the pipette...................................21
5.3 Modify existing programs ...............................................................21
5.4 Adjust heights and positions...........................................................22
5.4.1 Enter heights/positions directly ...........................................22
5.4.2 Teach heights/positions on ASSIST PLUS .........................22
5.5 Program offset................................................................................23
5.6 Pipetting alternating wells...............................................................24
5.7 Detailed description of predefined programs..................................25
5.7.1 Serial Dilution program .......................................................25
5.7.2 Repeat/Variable dispense programs...................................29
5.7.3 Multi aspirate program ........................................................32
5.7.4 Plate copy program.............................................................34
5.7.5 Reformat program...............................................................35
5.7.6 Custom programs ...............................................................37
Chapter 6 Maintenance
6.1 Cleaning .........................................................................................41
6.2 Decontamination ............................................................................41
6.3 Servicing.........................................................................................42
6.4 Equipment disposal ........................................................................42
Chapter 7 Technical Data
7.1 Environmental conditions ...............................................................43
7.2 Specification of the device..............................................................43
7.3 Intellectual property ........................................................................43
7.4 Compatible pipettes........................................................................44
7.5 Maximum labware height on deck..................................................44
7.6 Plate compatibility ..........................................................................45
7.7 Plate access pattern.......................................................................46
7.8 Tip travel distances ........................................................................47
Chapter 8 Accessories
8.1 Accessories ...................................................................................48
8.2 Consumables .................................................................................50
Imprint ...................................................................................................56
4 INTEGRA Biosciences AG
ASSIST PLUS – Operating instructions V02 Introduction
1 Introduction
These operating instructions contain all the information required for installation, operation
and maintenance of the ASSIST PLUS. This chapter informs about the symbols used in
these operating instructions, the intended use of the ASSIST PLUS and the general safety
instructions.
1.1 Symbols used
The operating instructions specifically advise of residual risks with the following symbols:
ARNING
W
This safety symbol warns against hazards that could result in injury. It also
indicates hazards for machinery, materials and the environment.
It is essential that you follow the corresponding precautions.
C
AUTION
This symbol cautions against potential material damage or the loss of data in
a microprocessor controller. Follow the instructions.
OTE
N
This symbol identifies important notes regarding the correct operation of the
device and labor-saving features.
The device is marked with the following symbols:
IOHAZARD
B
The instrument can be potentially biohazardous due to the use of
biohazardous substances by the operator.
C
RUSHING OF HANDS
The hands may be squeezed, pulled in or otherwise injured by moving parts
of the instrument.
MOVING PARTS
For correct operation, keep the tower moving area free of any
labware and keep hands away.
L
ASER CLASS 1
The sensor contains a class 1 laser which is inherently safe
under reasonably foreseeable conditions of operation.
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ASSIST PLUS – Operating instructions V02 Introduction
1.2 Intended use
This is a general-purpose laboratory instrument for use in research only. Any use of this
instrument in a medical or IVD setting is the sole responsibility of the user.
This product may only be operated in a secure, protected network with validated,
trustworthy clients. The operator must ensure that network security measures are always
up-to-date and state-of-the-art. This product may not be directly exposed to the internet.
If the ASSIST PLUS is used in a manner not specified by INTEGRA Biosciences, the
protection provided by the ASSIST PLUS may be impaired.With a VIAFLO electronic
multichannel or VOYAGER pipette attached, ASSIST PLUS performs pipetting operations
automatically (for pipette compatibility see 7.4
OTE
N
Each INTEGRA pipette needs its own Bluetooth module, which has to be
ordered separately (part no. 4221).
Electronic INTEGRA pipettes are microprocessor controlled and stepper motor driven
pipettes. They are used for aspirating and dispensing liquids in the volume range of 0.5 –
1250 µl using GripTip pipette tips. Please refer to the VIAFLO/VOYAGER electronic
pipettes operating instructions for more detailed informations on www.integrabiosciences.com.
).
6 INTEGRA Biosciences AG
ASSIST PLUS – Operating instructions V02 Introduction
1.3 Safety notes
ASSIST PLUS complies to the recognized safety regulations and is safe to operate.
ASSIST PLUS can only be operated when in intact condition and while observing these
operating instructions.
The device may be associated with residual risks if it is used or operated improperly by
untrained personnel. Any person operating the ASSIST PLUS must have read and
understood these operating instructions, and particularly, the safety notes, or must have
been instructed by supervisors so that safe operation of the device is guaranteed.
AUTION
C
Do not open or modify the ASSIST PLUS in any way. Repairs may only be
performed by INTEGRA Biosciences AG or by an authorized after-sales
service member. Parts may be replaced with original INTEGRA Biosciences
parts only.
ARNING
W
Do not use the ASSIST PLUS near flammable material or in explosive areas.
Also, do not pipette highly flammable liquids such as acetone or ether.
When handling dangerous substances, comply with the material safety data
sheet (MSDS) and with all safety guidelines such as the use of protective
clothing and safety goggles.
OTE
N
Prolonged exposure of the ASSIST PLUS to UV-light can cause
discoloration and/or yellowing. However, this will not affect the performance
of the device in any way.
Regardless of the listed safety notes, additional applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com
information regarding REACH classified chemicals contained in our products.
on a regular basis for up to date
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ASSIST PLUS – Operating instructions V02 Description of the device
1
4
3
7
12
13
9
6
10
11
2
8
14
5
2 Description of the device
2.1 Scope of delivery
• ASSIST PLUS (pipettes to be ordered separately, see “8.1 Accessories” on page 48)
• Mains adapter
• Tip waste bags sample pack
• Trial pack 10 ml, 25 ml and 100 ml multichannel reagent reservoir
• Trial pack 300 ml automation friendly reagent reservoir
• Pipette charging cable, tip waste bin with reflector for tip sensor
AUTION
C
Verify the scope of delivery when unpacking the device and check for
potential transportation damage. Do not operate a device that is damaged,
instead contact your local dealer.
2.2 Overview of the ASSIST PLUS
2.2.1 ASSIST PLUS base unit
1 Tower with Pipette arm.
Tower moves in X-direction,
Pipette arm in Z-direction.
2 Charging cable for pipette
3 Gripper with lever to release
the pipette.
4 Pipette arm with Pipette
holder, that moves in Ydirection.
5 LEDs for adjustable deck
illumination.
6 Tip rack deck for GripTips,
8 Reflector for tip sensor, removable.
9 Waste bin, for automatic tip ejection,
removable.
10 Tip sensor, for tip monitoring.
removable.
7 Deck, removable, with
Positions, e.g. A, B and C.
11 Interfaces and Main switch
12 Touch panel
13 Instrument base
14 Feet, adjustable.
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ASSIST PLUS – Operating instructions V02 Description of the device
14
15
16
17
18
Z
X
Y
(Tower: right and left)
(Pipette arm: up and down)
(Pipette holder: back and fore)
20
21
22
23
2.2.2 Touch panel
15 Back and Fore arrow
keys move the pipette along
the Y-axis.
16 Up and Down arrow keys
move the pipette along the Zaxis to teach pipetting heights.
Left and Right ar ro w keys
move the pipette along the Xaxis to access each position on
the deck.
17 Start/Pause key, to start/stop
operations.
18 Communication LED (on top)
and Error LED (at the bottom)
19 On/Standby key
Each key lights white as soon it can be used.
Key Light Information/Action
Arrow
, 16)
(15
Start/Pause
(17
)
Communication LED (18
Error LED
(18 at the
bottom)
On/Standby
(19
)
lights blue finger contact detected
flashes moving in this direction not allowed
flashes white press to start operation, homing or to quit error
lights white performing operation, press to pause
lights blue Bluetooth connection active
)
flashes red error not critical, press Start/Pause key or follow
the instructions on the pipette
lights red critical error, switch off ASSIST PLUS with On/
Standby key or Main switch
lights white ASSIST PLUS is switched on
inner symbol dims standby program, press to switch on
2.2.3 Interfaces
20 Socket for mains adapter
21 USB port, for firmware
updates
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22 AUX connection
23 Main switch
ASSIST PLUS – Operating instructions V02 Description of the device
25
28
29
30
31
26
27
32
33
24
34
Program Name
OK
OK
2.3 Overview of INTEGRA Electronic Pipettes
2.3.1 Pipette parts
24 Display
25 Back button, to navigate backward
26 Touch wheel, spin to scroll and move the cursor
27 OK button, to make a selection
28 Left and right arrow buttons, for selections
29 Purge button, to empty tips
30 Run key, to start operations
31 Tip ejector
32 Finger hook, facilitates easy operation
33 Volume indicator label, color matches GripTip box
insert
34 Tip ejector lid, remove before using the pipette with
ASSIST PLUS
2.3.2 Display
The Display shows all pipetting options.
Battery life indicator
10 INTEGRA Biosciences AG
select a
Program
category
and a
Program
Instruction
Options
ASSIST PLUS – Operating instructions V02 Installation
3 Installation
3.1 Operating environment
ASSIST PLUS has been designed for use in a laboratory. It shall be operated in a dry and
dust-free location with a temperature of 5–40°C and a maximal (non-condensing) relative
humidity of 80 %, see “7.1
3.2 Setting up and moving of the instrument
The ASSIST PLUS must be set up on a cleaned, dry and horizontal surface. Two persons
are required to lift the ASSIST PLUS. Hold the ASSIST PLUS firmly on both sides of the
Instrument base (13
ARNING
W
Never lift the instrument on the Pipette arm (4), the Gripper (3) or on the Tip
sensor (10).
It must always be possible to manually disconnect the plug of the
ASSIST PLUS from electricity supply. The corresponding socket shall be
within easy reach of the operator and be clearly labeled as the disconnecting
device of ASSIST PLUS.
Only use a 3 core mains cable with protective earth to connect the external mains adapter
of ASSIST PLUS.
Relocating
ARNING
W
ASSIST PLUS must be secured in the park position before carrying.
Environmental conditions” on page 43.
).
Before ASSIST PLUS can be relocated the tower must be parked. Clear both Decks (6
) of all labware and remove the Waste bin (9). Remove the Deck (7) or pull both levers
7
of the Pipette holder (4) down. Set the instrument to park position: Press the Back and
Fore arrow keys (15) and then the On/Standby key (19) simultaneously for approx. 3
seconds until the tower moves to park position. Alternatively, select “MOVE to park
position” within the Toolbox of ASSIST PLUS and press the Run key (30
Switch off the device and disconnect it from the electricity mains.
).
3.3 Charging the battery of the electronic pipette
The battery indicator in the upper right corner of the pipette screen informs about the
battery status. When it turns red, the pipette needs to be charged.
CAUTION
Use only the approved INTEGRA battery, power supply and charging stand.
Use of an incompatible power transformer can damage the pipette.
The battery can be charged using the mains adapter, a charging stand or the pipette
Charging cable (2) on the ASSIST PLUS, see “8.1 Accessories” on page 48.
www.integra-biosciences.com 11
,
ASSIST PLUS – Operating instructions V02 Installation
a
b
3.4 Removing the tip ejector lid
For automatic tip ejection, remove the Tip ejector lid
(34
) with your thumb nail and store it in a safe place
(pipettes with serial number ≥7 000 000 only).
3.5 Adapting the pipette holder
The pipette holder can be adapted to accommodate INTEGRA electronic multichannel
pipettes.
To adapt the holder for an 8- or 16-channel VIAFLO
pipette fold both silver levers (a, b) down as shown
beside.
To adapt the holder for a 12-channel VIAFLO pipette,
only pull the lower silver lever (b) down.
Fold both levers up to insert a VOYAGER pipette.
3.6 Attaching and removing an electronic pipette
Rotate the lower hosing of the electronic pipette by 90 degrees as displayed in the picture
below.
AUTION
C
The VOYAGER pipette may only be
rotated clockwise to 90 degrees.
To install a pipette, the Tip ejector opening (34
must face the Pipette holder (4
Hold the pipette at an angle and insert it into the
pipette holder.
Then lift up until the black gripper snaps in place.
12 INTEGRA Biosciences AG
).
)
ASSIST PLUS – Operating instructions V02 Installation
a
To release the pipette lift the black gripper by pushing
down the left end.
3.7 Inserting decks
Hold the front and the back of the Tip box deck (6
find the correct orientation and place it over the
corresponding positioning studs. Press down firmly
until you hear a click.
Hold the Deck (7
the corresponding positioning studs. Press down
firmly until you hear a click.
) with both hands and place it over
3.8 Inserting waste bags and waste bin
Remove the frame from Waste bin (9) and insert a waste bag (see “8.2 Consumables” on
page 50) into the bin.
Place the opening of the waste bin frame on the
centering bar (a) of the Waste bin.
Lower the frame onto the bin while holding the bag to
the side wall of the Waste bin. The bag is now fixed.
Attach the Sensor reflector (8
pins into the two holes of the frame. The reflector is
Place the Waste bin on the Instrument base (13
The Sensor reflector (8
) must face the Tip sensor (10), as shown in the picture above.
fixed magnetically.
) on the right side next to the tip sensor.
) by inserting the two
),
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ASSIST PLUS – Operating instructions V02 Installation
3.9 Toolbox settings
3.9.1 Enabling ASSIST PLUS program
By default the ASSIST PLUS program is hidden from the Main Menu.
Use the Tou ch wheel to s elec t Toolbox fr om th e Mai n Men u an d pre ss
OK.
Select Preferences and then Main Menu. Enable the ASSIST PLUS
program by pressing OK (green ) and press to save your settings.
3.9.2 Overview of Toolbox for ASSIST PLUS
The Toolbox for ASSIST PLUS provides options to adapt the pipette to appropriate
applications.
Toolbox Description
Tip Monitor Defines whether loaded tips should be checked directly after tip
loading, before tip ejection and after tip ejection.
Tip Type Sets the tip type standard, short or long (only for 12.5 μl
pipettes). Note: Tip definitions in VIALAB programs will not be
overwritten by this setting.
Preferences Deck brightness sets brightness of deck illumination.
Device Information Contains Information about the instrument and the software.
Bluetooth Pairing Pairs the INTEGRA pipette and the ASSIST PLUS.
Move to park position Fixes the tower and the instrument base for safe transportation.
Move to reference
pos.
Position Adjustment Sets an offset of the tip rack deck.
Moves the tower to the reference position to verify correct
alignment. Adjustment Tool required, for INTEGRA staff only.
3.9.3 Device Information
This section contains information about your ASSIST PLUS, such as serial number,
firmware (FW) and hardware (HW) version and error status.
14 INTEGRA Biosciences AG
ASSIST PLUS – Operating instructions V02 Installation
3.9.4 Bluetooth Pairing
The first time a specific pipette is connected to the ASSIST PLUS, both instruments need
to be paired via Bluetooth. (Alternatively, the pipette can be connected using the
communication cable, see 3.4.) Scroll to the Toolbox and press OK.
From the ASSIST PLUS option select Bluetooth Pairing.
Switch OFF and ON the ASSIST PLUS device, see “4.1 Turn on/off
the ASSIST PLUS device” on page 17, and wait approx. 30 sec. until
the message “Pairing successful” is displayed. Press OK.
3.9.5 Tip Monitor
Define whether the tip loading should be checked after tip loading, before tip ejection or
after tip ejection.
Select Tip Monitor and press OK.
Use the Touch wheel to highlight an option. Press OK to toggle
between on (green ) and off (red ). Save your settings.
N
OTE
It is recommended to keep tip monitoring settings Before and After Tip Eject
active.
3.9.6 Setting tip type
Electronic Pipettes of 12.5, 300 and 1250 µl sizes can be used with standard, SHORT and
LONG GripTips. In order to teach the correct heights for ASSIST PLUS movements, the
used tip type must be specified. Go to the Toolbox of the pipette, select ASSIST PLUS and
press OK.
Select Tip Type and press OK.
Use the Touch wheel to highlight Standard, SHORT or LONG. Press
OK to select the correct tip type (green ) and Save your settings.
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ASSIST PLUS – Operating instructions V02 Installation
X
Y
Z
3.9.7 Position adjustment
The Position Adjustment option is used to set an offset for the absolute X/Y/Z-coordinates
in mm to adjust the tip rack position. Select ASSIST PLUS on the Toolbox and press OK.
Select Position Adjustment and press OK.
Use the Touch wheel to select Tip Rack and press
OK.
Set the offset(s) in mm of the selected option and
Save your settings.
X: positive value moves to the right
Y: positive value moves to the rear
16 INTEGRA Biosciences AG
ASSIST PLUS – Operating instructions V02 Operation
4 Operation
4.1 Turn on/off the ASSIST PLUS device
Connect ASSIST PLUS to the power supply with the supplied mains adapter. Turn on
ASSIST PLUS using the Main switch (11).
Press the Start/Pause key (17
ARNING
W
Remove hands from ASSIST PLUS during homing.
After homing the LED of the Start/Pause key will turn off.
To switch ASSIST PLUS to standby press the On/Standby key (19
its LED dims. The ASSIST PLUS automatically switches in standby program after 2 hours
of inactivity. Press the On/Standby key again to return in active program.
Use the Main switch to turn off the instrument.
4.2 Turn on/off an INTEGRA electronic pipette
Press and release the Run key (30) to turn on the pipette.
To turn off the pipette, press and hold the Back button (25
4.3 Connection ASSIST PLUS to Pipette
4.3.1 Bluetooth connection
Select the ASSIST PLUS program in the Main Menu of the INTEGRA
pipette and press OK. The pipette establishes the Bluetooth
connection.
When the blue Bluetooth symbol ( ) next to the battery indicator is
displayed and the Communication LED (18
instruments are connected.
If the connection fails, press to retry the connection or refer to “4.5 Troubleshooting” on
page 19. Alternatively, continue Offline . In offline program you cannot run programs but
you can view and edit them. Active height teaching is not possible in offline program.
) when it is blinking to home the ASSIST PLUS.
) for two seconds until
) for 3 seconds.
) lights up in blue, both
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