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ASSIST Operating instructions
127950_V09
Page 2
Declaration of conformity
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the device
complies with:
Zizers, April 13, 2018
Description Model
ASSIST 4500
EU Directives (DoW: Date of Withdrawal) Before DoW DoW After DoW
Low Voltage Equipment 2006/95/EC 20.04.2016 2014/35/EU
Electromagnetic Compatibility 2004/108/EC 20.04.2016 2014/30/EU
Restriction of Hazardous Substances 2011/65/EU
Waste Electrical and Electronic Equipment 2012/19/EU
Radio Equipment Directive 1999/5/EC 13.06.2016 2014/53/EU
EU Regulations
Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH)
1907/2006
Ecodesign - Power supplies 278/2009
Standards for EU
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
EN 61010-1: 2010
Particular requirements for automatic and semi-automatic
laboratory equipment for analysis and other purposes.
EN 61010-2-81: 2015
Electrical equipment for measurement, control and laboratory
use - EMC requirements.
EN 61326-1: 2013
Standards for Canada and USA
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
CAN/CSA-C22.2
No. 61010-1
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
UL 61010-1
Particular requirements for automatic and semi-automatic
laboratory equipment for analysis and other purposes.
UL 61010-2-81
Operation is subject to the following two conditions:
(1) this device may not cause harmful interference, and
(2) this device must accept any interference received, including
interference that may cause undesired operation.
Part 15 of the
FCC Rules
Contains FCC ID:
PI4410B
Elmar Morscher
CEO
Thomas Neher
Quality Manager
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ASSIST – Operating instructions V09
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Table of Contents
Chapter 1 Introduction
1.1 Symbols used...................................................................................5
1.2 Intended use.....................................................................................6
1.3 Safety notes .....................................................................................7
Chapter 2 Description of the device
2.1 Scope of delivery..............................................................................8
2.2 Overview of the ASSIST...................................................................8
2.2.1 ASSIST device......................................................................8
2.2.2 Keypad..................................................................................8
2.3 Overview of VIAFLO-Pipetten ..........................................................9
2.3.1 VIAFLO pipette parts ............................................................9
2.3.2 Display ..................................................................................9
Chapter 3 Installation
3.1 Operating environment...................................................................10
3.2 Relocating ASSIST.........................................................................10
3.3 Charging the battery of the VIAFLO Electronic Pipette ..................10
3.4 Adapting the pipette holder ............................................................10
3.5 Attaching and removing a VIAFLO Electronic Pipette....................11
3.6 Toolbox settings .............................................................................11
3.6.1 Enabling ASSIST mode ......................................................11
3.6.2 Overview of Toolbox for ASSIST ........................................12
3.6.3 Device Information..............................................................12
3.6.4 Connection between ASSIST and pipette...........................12
3.6.5 Setting tip type ....................................................................12
3.6.6 Adjusting the plate alignment..............................................13
3.6.7 Move Speed........................................................................13
Chapter 4 Operation
4.1 Turn on/off the ASSIST device.......................................................14
4.2 Turn on/off the VIAFLO Electronic Pipette .....................................14
4.3 Bluetooth connection ......................................................................14
4.4 Running a program.........................................................................14
4.5 Pipetting in 96 and 384 well plates.................................................15
4.6 Troubleshooting..............................................................................16
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Chapter 5 Programming
5.1 Overview program categories ........................................................17
5.2 Setting up a program......................................................................18
5.3 Modify existing programs ...............................................................18
5.4 Adjust heights and positions...........................................................19
5.4.1 Enter heights/positions directly ...........................................19
5.4.2 Teach heights/positions on ASSIST ...................................19
5.5 Detailed description of program categories ....................................20
5.5.1 Serial Dilution program .......................................................20
5.5.2 Repeat/Variable dispense programs...................................22
5.5.3 Multi Aspirate program........................................................24
5.5.4 Custom programs ...............................................................25
Chapter 6 Maintenance
6.1 Cleaning .........................................................................................27
6.2 Decontamination ............................................................................27
6.3 Servicing.........................................................................................27
6.4 Equipment disposal ........................................................................27
Chapter 7 Technical Data
7.1 Environmental conditions ...............................................................28
7.2 Specification of the device..............................................................28
7.3 Intellectual Property........................................................................28
7.4 Compatible pipettes........................................................................29
7.5 Maximum labware height on plate carrier ......................................29
7.6 Tip travel distances ........................................................................30
Chapter 8 Accessories
8.1 Accessories for ASSIST .................................................................31
8.2 Accessories for VIAFLO-Pipettens.................................................31
8.3 Consumables .................................................................................32
Imprint ...................................................................................................35
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ASSIST – Operating instructions V09 Introduction
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1 Introduction
These operating instructions contain all the information required for installation, operation
and maintenance of the ASSIST. This chapter informs about the symbols used in these
operating instructions, the intended use of the ASSIST and the general safety instructions.
1.1 Symbols used
The operating instructions specifically advise of residual risks with the following symbols:
W
ARNING
This safety symbol warns against hazards that could result in injury. It also
indicates hazards for machinery, materials and the environment.
It is essential that you follow the co rresponding precautions.
C
AUTION
This symbol cautions against potential material damage or the loss of data in
a microprocessor controller. Follow the instructions.
NOTE
This symbol identifies important notes regarding the correct operation of the
device and labor-saving features.
The device is marked with the following symbol:
B
IOHAZARD
The instrument can be potentially biohazardous due to the use of
biozazardous substances by the operator.
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1.2 Intended use
This is a general-purpose laboratory instrument for use in research only. Any use of this
instrument in a medical or IVD setting is the sole responsibility of the user.
If the ASSIST is used in a manner not specified by INTEGRA Biosciences, the protection
provided by the ASSIST may be impaired.
With a VIAFLO electronic multichannel pipette attached, ASSIST performs pipetting
operations in microplates automatically.
N
OTE
Only VIAFLO multichannel pipettes (as of serial number 600 000 and
firmware version 3.00 or higher) can be used with ASSIST. The VIAFLO
pipette needs its own Bluetooth module, which has to be ordered separately
(part no. 4221).
VIAFLO Electronic Pipettes are microprocessor controlled and stepper motor driven
pipettes. They are used for aspirating and dispensing liquids in the volume range of 0.5–
1250 µl using GripTip pipette tips. Please refer to the VIAFLO Electronic Pipettes
operating instructions for more detailed informations on www.integra-biosciences.com
.
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1.3 Safety notes
ASSIST complies to the recognized safety regulations and is safe to operate. ASSIST can
only be operated when in intact condition and while observing these operating
instructions.
The device may be associated with residual risks if it is used or operated improperly by
untrained personnel. Any person operating the ASSIST must have read and understood
these operating instructions, and particularly, the safety notes, or must have been
instructed by supervisors so that safe operation of the device is guaranteed.
C
AUTION
Do not open or modify the ASSIST in any way. Repairs may only be
performed by INTEGRA Biosciences AG or by an authorized after-sales
service member. Parts may be replaced with original INTEGRA Biosciences
parts only.
W
ARNING
Do not use the ASSIST near flammable material or in explosive areas. Also,
do not pipette highly flammable liquids such as acetone or ether.
When handling dangerous substances, comply with the material safety data
sheet (MSDS) and with all safety guidelines such as the use of protective
clothing and safety goggles.
N
OTE
Prolonged exposure of the ASSIST to UV-light can cause discoloration and/
or yellowing. However, this will not affect the performance of the device in
any way.
Regardless of the listed safety notes, additional applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com
on a regular basis for up to date
information regarding REACH classified chemicals contained in our products.
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ASSIST – Operating instructions V09 Description of the device
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2 Description of the device
2.1 Scope of delivery
• ASSIST (VIAFLO-Pipetten to be ordered separately)
• Mains adapter
• Sample pack 10 ml, 25 ml and 100 ml reagent reservoir
C
AUTION
Verify the scope of delivery when unpacking the device and check for
potential transportation damage. Do not operate a device that is dama ged,
instead contact your local dealer.
2.2 Overview of the ASSIST
2.2.1 ASSIST device
2.2.2 Keypad
1 Tower with pipette arm. Arm moves in Z-direction
2 Plate carrier, moves in X-direction
3 Power connection
4 USB port, for firmware updates
5 Keypad
6 Reservoir position
7 Instrument base
8 Pipette holder on pipette arm with lever to shift the
pipette in Y-direction
9 Gripper, lever to release the pipette
10 VIAFLO Electronic Pipette
11 Up and Down arrow keys move the pipette
along the Z-axis to teach pipetting heights.
12 Left and Right arrow keys move the plate
carrier along the X-axis to adjust the plate carrier
position.
13 Start/Pause key, to start/stop operations.
Green LED blinks: press to start operation/homing
Green LED lights: operation performed
14 Bluetooth control LED (lights blue if connection is
active) and Error LED (flashes red in case of error)
15 On/Off key (lights green if On)
3
9
5
6
8
2
10
1
7
4
11
12
13
14
15
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2.3 Overview of VIAFLO-Pipetten
2.3.1 VIAFLO pipette parts
2.3.2 Display
The Display shows all pipetting options.
16 Display
17 Back button, to navigate backward
18 Touch wheel, spin to scroll and move the cursor
19 OK button, to make a selection
20 Left and right arrow buttons, for selections
21 Purge button, to empty tips
22 Run key, to start operations
23 Tip ejector
24 Finger hook, facilitates easy operation
25 Volume indicator label, color matches GripTip box
insert.
Battery life indicator
Instruction
Volume
Options
16
17
20
21
22
23
18
19
24
25
Current category
Active step
OK - Select
a program
category and
a program
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3 Installation
3.1 Operating environment
ASSIST has been designed for use in a laboratory. It shall be operated in a dry and dustfree location with a temperature of 5–40°C and a maximal (non-condensing) relative
humidity of 80 %, see “7.1
Environmental conditions” on page 28.
3.2 Relocating ASSIST
Before ASSIST can be relocated, clear the Instrument base (7). Swi tch off the devi c e and
disconnect it from the electricity mains. Lift the ASSIST on both sides of the Instrument
base. Never lift the instrument on the Tower.
3.3 Charging the battery of the VIAFLO Electronic Pipette
The battery indicator in the upper right corner of the pipette screen informs about the
battery status. When it turns red, the pipette needs to be charged.
C
AUTION
Use only the approved INTEGRA battery, power supply or charging stand.
Use of an incompatible power transformer can damage the pipette.
The battery can be charged using either the mains adapter or a charging stand, see (“8.2
Accessories for VIAFLO-Pipettens” on page 31).
3.4 Adapting the pipette holder
The pipette holder can be adapted to the type of the VIAFLO pipette.
Insert the mains adapter connector into the receptacle on the top
back of the pipette. Plug the mains adapter into a wall outlet.
To adapt the holder for an 8- or 16-channel pipette, pull the silver
lever down as shown beside.
Lift the lever up to insert a 12-channel pipette.
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3.5 Attaching and removing a VIAFLO Electronic Pipette
Rotate the lower hosing of the VIAFLO Electronic Pipette by 90 degrees as displayed in
the picture.
3.6 Toolbox settings
3.6.1 Enabling ASSIST mode
The ASSIST mode is hidden from the Main Menu of the VIAFLO pipettes by default.
To install a pipette, hold it at an angle and insert it into the pipette
holder.
Then lift up until the black gripper snaps in place.
To release the VIAFLO Electronic Pipette lift the black gripper by
pushing down on one end.
Use the Touch w h e e l to selec t Toolbox from the Main Me nu and p ress
OK.
Select Preferences and then Main Menu. Enable the ASSIST mode by
pressing OK (green ) and press to save your settings.
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3.6.2 Overview of Toolbox for ASSIST
The Toolbox for ASSIST provides options to adapt the pipette to appropriate applications.
3.6.3 Device Information
This section contains information about your ASSIST, such as serial number, firmware
(FW) and hardware (HW) version and error status.
3.6.4 Connection between ASSIST and pipette
The first time a specific VIAFLO pipette is going to be connected to the ASSIST, both
instruments need to be paired. Scroll to the Toolbox and press OK.
3.6.5 Setting tip type
Some pipette tips of the same volume are available in different lengths (e.g. 12.5 µl in
short, standard and long version). In order to teach the correct heights for ASSIST, the
used tip type must be specified. Go to the Toolbox of the VIAFLO pipette, select ASSIST
and press OK.
Toolbox mode Description
Plate Alignment Aligns the pipette's tips along the X-axis with the center of the
wells in the first row/column of a microplate.
Tip types Sets the tip type standard, short or long (only where applicable).
Device Information Contains information about the instrument and the software.
Bluetooth Pairing Pairs the INTEGRA pipette and the ASSIST.
Move Speed Allows to change the move speed of the pipette arm and the
plate carrier.
From the ASSIST option select Bluetooth Pairing.
Switch OFF and ON the ASSIST device, see “4.1
Turn on/off the
ASSIST device” on page 14, and wait approx. 30 sec. until the
message “Pairing successful” is displayed. Press OK.
Select Tip Type and press OK.
Use the Touch wheel to highlight Standard or Long. Press OK to
select the correct tip type (green ) and Save your settings.
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3.6.6 Adjusting the plate alignment
The Plate Alignment option is used to align the pipette's tips along the X-axis with the
center of the wells in the first row/column of a microplate (depending on plate orientation).
Select ASSIST on the Toolbox and press OK.
With GripTips attached, install the VIAFLO pipette on the ASSIST and put a plate on the
plate carrier. Use the Left arrow key to positon row A / column 1 below the tips. Press
the arrow keys until the tips target the center of the wells. Press Set to save this position.
N
OTE
Plate alignments with 384 well plates require a 16-channel VIAFLO-Pipetten.
When working with 8- or 12-channel pipettes in a 384 well plate, the plate
alignment is made on a 96-well pl a te nevertheless.
3.6.7 Move Speed
Allows to change the move speed of the pipette arm (1
, Z-axis) and the plate carrier (2, X-
axis) on ASSIST.
Select Plate Alignment and press OK.
Define the number of wells of your plate (e.g. 96 for a
96-well plate) and choose the plate orientation from
either landscape or portrait. Use the Touch wheel to
select an option and press OK.
For fine adjustment of the pipette tips along the Y-axis turn the
screw on the pipette holder (a) with a flathead screw driver size 2.
Turning clockwise moves the pipette to the rear, counterclockwise
to the front. The maximum Y-shift is +/- 5 mm.
Select Move Speed and press OK.
Use the Touch wheel to select Slow, Medium or Default (=fast) and
press OK. Save your settings.
a
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4 Operation
4.1 Turn on/off the ASSIST device
Connect ASSIST to the power supply with the supplied mains adapter. Turn on ASSIST
by pressing the On/Off key (15), which is confirmed by the green LED lighting up.
Press the Start/Pause key when it is blinking to home the ASSIST device.
W
ARNING
Remove hands from ASSIST during homing.
After homing the LED of the Start/Pause key will turn off.
To turn off ASSIST press the On/Off key two seconds. It’s LED switches off.
4.2 Turn on/off the VIAFLO Electronic Pipette
Press and release the Run key (22) to turn on the pipette.
To turn off the pipette, press and hold the Back button (17
) for 3 seconds.
4.3 Bluetooth connection
If the connection fails, press to retry the connection or refer to “4.6 Troubleshooting” on
page 16. Alternatively, continue Offline . During offline mode ASSIST cannot be
operated. It is possible to create programs, however without teaching of active heights.
4.4 Running a program
Select the ASSIST mode in the Main Menu of the VIAFLO pipette and
press OK. The pipette establishes the Bluetooth connection.
When the blue Bluetooth symbol ( ) next to the battery indicator is
displayed and the Bluetooth control LED (14
) alights, both
instruments are connected.
From the ASSIST menu select the program category and the stored
program you want to run and press OK.
Press the Run key (22).
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You are prompted to place the pipette on the ASSIST device, see “3.5 Attaching and
removing a VIAFLO Electronic Pipette” on page 11. When attached press the green
blinking Start/Pause key (13
) on the ASSIST device. It switches to solid green and the
program will be performed automatically.
W
ARNING
Keep hands out of area of moving ASSIST parts during the run.
A program can be interrupted by pressing the Start/Pause key (13
). Either press the
Start/Pause key again to continue the program or press Abort on the pipette to abort
the program.
4.5 Pipetting in 96 and 384 well plates
The standard option to fill a 384 well plate is to use a 16-channel pipette. To access a 384
well plate with an 8- or 12-channel pipette, the pipette needs to be shifted in Y-direction.
To do so, switch the lever (a) on the pipette holder to the front or rear position. The center
position is used for 96 well plates.
The rear position of the
lever (a) moves the pipette
to the rear to accommodate the rear positions of
384 well plates.
Set the lever (a) to the
center to pipette in 96 well
plates.
The front position of the
lever (a) moves the pipette
to the front to accommodate the front positions of
384 well plates.
aaa
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4.6 Troubleshooting
Problem Probable cause Remedy
The connection
between pipette and
ASSIST cannot be
established.
• The two instruments have not
been paired or the pairing
was lost.
• From the main menu of the
pipette go to Toolbox -> ASSIST
and select Bluetooth Pairing,
see “3.6.2
Overview of Toolbox
for ASSIST” on page 12. Follow
the instructions on the pipette
screen.
Error LED (14)
blinks red
• ASSIST motor lost steps
during movement.
• Pipetting heights were set
wrong and the pipette
crashed into the plate.
• Follow the instructions on the
pipette display.
After firmware
update, the Error
LED (14
) blinks red
and ASSIST cannot
be started.
• No firmware installed. • Contact service for VIALINK
special firmware update.
GripTips are not
aligned in X-position.
• Plate alignment not set. • Perform plate alignment, see
“3.6.6
Adjusting the plate
alignment” on page 13.
The tip height is not
correct using 12.5 μl
GripTips.
• Wrong tip type set. • Enter the tip type used, see
“3.6.5
Setting tip type” on
page 12.
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5 Programming
5.1 Overview program categories
The table below shows program categories which can be performed automatically in
combination with ASSIST. All categories are accessed from the ASSIST Menu of a VIAFLO-Pipetten. You can create and store up to 20 different programs in each of Serial Dilution, Repeat Dispense, Variable Dispense or Multi Aspirate category and up to 40 different
Custom programs.
Program categories Description
Serial Dilution Allows aspirating a transfer volume followed by a mix. Columns
and Mix Cycles are tracked on the display.
Repeat Dispense Allows dispensing multiple aliquots of the same volume without
refilling the tips after each dispense for fast microplate filling and
processing.
Variable Dispense Allows dispensing multiple aliquots of different volumes.
Multi Aspirate Allows aspirating multiple aliquots of the same or different
volumes from a microplate.
Custom Programs Allows to create and store multi-stepped user-defined pipetting
protocols.
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5.2 Setting up a program
NOTE
If no Bluetooth connection is available, you can also use the offline mode to
create a new program. Live teaching of pipetting heights is not possible in
offline mode.
To run the program, select the stored program and press OK on the VIAFLO-Pipetten, see
4.4 “
Running a program” on page 14.
5.3 Modify existing programs
Use the Touch wheel to scroll to your desired program category and
press OK.
Press New to create a new program. Your are prompted to enter a
name.
Use the Tou c h wheel to select characters and press OK. Once
finished, press to save the name. The programs can later be
renamed, see 5.3 “
Modify existing programs” on page 18.
Define all parameters of your program and press Save .
At any program display, use the Touch wh e e l to highlight an existing
program.
Press Options, use the Touch wheel to select an option (View/Edit,
Delete, Copy, Rename) to modify the program and press OK.
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5.4 Adjust heights and positions
5.4.1 Enter heights/positions directly
Open a predefined or a custom program and scroll to any Height setting or select any
Move step and the desired position respectively. Press OK to display the setting dial
showing the current height/position.
• Reservoir or Tube Strip: The height is the distance between the end of the GripTips
and the Instrument base (7).
• Column or Row: If the plate or container is located on the Plate carrier (2
), the lower
point of reference is the Plate carrier (2).
Press OK and Save/Set to save the settings.
5.4.2 Teach heights/positions on ASSIST
If height and position values are unknown, they can be set in an active teaching mode.
Insert a pipette into the Pipette holder and establish a Bluetooth connection.
N
OTE
Perform teaching of all position settings with GripTips attached. Do not forget
to select the appropriate tip type where applicable, see 3.9.5.
Move the GripTips in the teach position:
• Reservoir: move the plate carrier out of the way with the Right arrow key of ASSIST.
• Column or Row: If the plate or container is located on the Plate carrier (2
), use the
Left and Right arrow keys of ASSIST to move the plate carrier into position, so
that the pipette tips target above the center of any wells or the container.
• When the setting dial is displayed as described above, press the Up and Down
keys to position the GripTips at the desired height and press OK to save current
settings.
N
OTE
Keep the Arrow keys (, , , ) pressed, if you want to increase the
movement speed during position teaching. Press the Arrow keys shortly for
fine adjustment.
If the exact values of heights or X/Z positions are
known, they can be entered directly using the Touch
Wheel.
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5.5 Detailed description of program categories
Use the Touch wheel to select an option, define the required parameters and press OK.
Press OK to toggle between on (green ) and off (red ). If a parameter is out of range,
the pipette beeps. Press Error to read the error message.
5.5.1 Serial Dilution program
Application: Use this program to perform serial dilutions. It enables aspiration of a
specific volume followed by a dispense and mix.
Options Steps Description of Serial Dilution
Plate Wells Sets the type of well plate (6 to 384) to be used as (source)/
target.
Orientation Sets the orientation of the plate (landscape, portrait).
Note: Switching to portrait requires to adapt default
parameters of "Count" and "First Row/Column".
Clear Height Sets the travel height at which the GripTips move above the
plate(s).
Source Location Sets the source container where the initial sample is
aspirated from:
• Reservoir: reagent reservoir
• Tube Strip: for PCR tubes
• Tip: samples can be aspirated before the automatic program is started.
• Column 1-24 or Row A-P: the sample is in the selected
column/row of the plate.
Height Sets the sample aspiration height of the source container.
Aspirate
Sets the sample volume that will be transferred from well to well.
Asp Speed Sets speed uniquely for aspiration (1 = low, 10 = fast).
Mixing Mixes the sample in the source container before the first
aspiration.
• If activated, define Mix volume, Mix Speed and number of
Mix Cycles.
Portrait
Pipette Rows
Landscape
Pipette Columns
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Press to save your settings. This will return you to the list of Serial Dilution programs.
Target First Column
or First Row
Sets the destination for the first transfer of the serial dilution
(column 1-24 or row A-P).
Columns or
Rows
Sets the number of columns or rows to dilute (1-n, including
the first column/row).
Height This is the dispense, mix and aspiration height in the target
container. If Tip Travel is enabled, this height is used as base
from which the tip travel starts, see 7.8 “Tip travel distances”
on page 45.
Mix Sets the mixing volume after dispensing. It does not affect
the transfer volume.
Mix Speed Sets the mixing speed (1 = low, 10 = fast).
Mix Cycles Sets the number of mixes per well (1-30).
Last
Aspirate
Location Choose the destination where the last aspiration should be
purged:
• Reservoir: the reagent reservoir serves as waste position
• Tube Strip: for PCR tubes
• Tip: last aspiration remains in the GripTips and is purged
manually
• Column 1-24 or Row A-P: the last aspiration is dispensed
in the selected column/ row of the plate.
Disp. Speed Set speed uniquely for last dispense (1 = low, 10 = fast).
BlowOut at The BlowOut is following the last dispense and expels extra
air to discharge residual liquid from the tips. Adjust the blow
out height, see 5.4 “
Adjust heights and positions” on page 19.
Advanced
Tip Travel Defines the distance which the tips move during an aspira-
tion, dispense and mix step to artificially track the liquid level,
see 7.8 “Tip travel distances” on page 45.
• Source Asp: moves the set distance down during aspiration in the source location.
• Source Mix: moves the set distance down and up during
mixing in the source location.
• Target Disp: moves the set distance up during dispensing
in the target location. The same distance is used to move
down during aspiration in the target location.
• Target Mix: moves the set distance down and up during
mixing in the target location.
Options Steps Description of Serial Dilution
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5.5.2 Repeat/Variable dispense programs
Application: These programs can be used for fast reagent addition to microplates from
one source container.
The settings for Variable dispense are identical to Repeat dispense except the dispense
steps in the target can have different volumes.
Options Steps Description of Repeat/Variable Dispense
Plate Wells Sets the type of well plate (6 to 384) to be used as (source)/
target.
Orientation Sets the orientation of the plate (landscape, portrait).
Note: Switching to portrait requires to adapt default parameters of "Count" and "First Row/Column"”.
Clear Height Sets the travel height at which the GripTips move above the
plate(s).
Source Location Sets the source container where the initial sample is
aspirated from:
• Reservoir: reagent reservoir
• Tube Strip: for PCR tubes
• Tip: samples can be aspirated before the automatic program is started.
• Column 1-24 or Row A-P: the sample is in the selected
column/row of the plate.
Tracking If Reservoir is selected and Tracking is active, the pipette
automatically adjusts the pipetting height according to the
defined tip immersion depth. Set the:
• Plate count: the total number of target plates (1-9) used in
the current run
• Reservoir type, only INTEGRA reservoirs, see 8.3
.
• Start volume: the filling volume that is currently in the
INTEGRA reagent reservoir.
• Tip immersion: the approx. immersion depth of the pipette
tips (2-3 mm are recommended).
Start Height The height where aspiration starts. The ASSIST will
automatically refill the GripTips if the total dispense volume
required for the target exceeds the maximum volume of the
pipette. The first aspiration will be at the start height and for
consecutive aspiration steps the pipette will go lower until it
reaches the end height.
Note: only visible if Tracking is switched off.
End Height Sets the height for the last aspiration step.
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ASSIST – Operating instructions V09 Programming
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Press to save your settings. This will return you to the list of Repeat/Variable Dispense
programs.
Source Asp Speed Sets speed uniquely for aspiration (1 = low, 10 = fast).
Mixing Mixes the sample in the source container before each
aspiration.
• If on, define Mix volume, Mix Speed and number of Mix
Cycles.
Dispense Pre-Dispense A Pre-Dispense volume can be selected independently,
which is discarded back to the source immediately after
aspiration, to improve accuracy and precision.
• If on, define the Pre-Dispense volume. Suggested: at least
4% of the pipette's maximum volume.
Count Sets the total number of dispensing steps.
First Column
or First Row
Sets the column/row where dispensing of the first dispense
should be started (column 1-24 or row A-P).
Dispense Only Repeat dispense:
Sets the volume to be dispensed in each well. The total
aspiration volume is calculated automatically. The pipette
cannot be overfilled.
Dispense
1 to n
Only Variable dispense:
Set the different dispense volumes for every variable
dispense step.
Disp. Speed Sets speed of all dispensing steps (1 = low, 10 = fast).
Height Sets the height for the dispensing steps.
TipTouch It is highly recommended to activate a tip touch after a
dispensing step to remove drops that may cling to the pipette
tips.
• Sets where the tip touch should be performed.
“Liquid”: the tips will dip into the center of the wells.
“Side”: the tips will touch to the side of the wells.
• Defines the height for the tip touch (“TipTouch at”).
Dispense Post-
Dispense
A Post-Dispense volume can be selected independently,
which is discarded, to improve accuracy and precision.
If on, define:
• the location where the Post-Dispense should be discarded
(Source or Tip) and
• the Post-Dispense volume. Suggested: 12.5 µl: 1 µl (= 8%),
50 µl: 2 µl (= 4%), 125 µl: 5 µl (= 4 %), 300 µl: 15 µl (= 5 %),
1250 µl: 50 µl (= 4%).
Options Steps Description of Repeat/Variable Dispense
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ASSIST – Operating instructions V09 Programming
24 INTEGRA Biosciences AG
5.5.3 Multi Aspirate program
Application: This program can be used for pooling applications or removal of
supernatants.
Press to save your settings. This will return you to the list of Multi Aspirate programs.
Options Steps Description of Multi aspirate
Plate Wells Sets the type of well plate (6 to 384) to be used as source/
(target).
Orientation Sets the orientation of the plate (landscape, portrait).
Note: Switching to portrait requires to adapt default parameters of "Count" and "First Row/Column".
Clear Height Sets the travel height at which the GripTips move above the
plates.
Aspirate Count Sets the total number of aspiration steps.
First Column
or First Row
Define the column/row where the first volume should be
aspirated from.
Repeat
Vol ume
If activated, identical volumes are aspirated.
• If off, define the different volumes for aspiration.
Aspirate
(1 to n)
Set the volume(s) to be aspirated (for every aspirate step).
Asp Speed Sets speed uniquely for aspiration (1 = low, 10 = fast).
Height Set the (start) Height for all aspiration (and mixing) steps.
Mixing Mixes the sample in the source container before each
aspiration.
• If on, define Mix volume, Mix Speed and number of Mix
Cycles.
Dispense Location Sets the target container where the sample is dispensed:
• Reservoir: reagent reservoir
• Tube Strip: for PCR tubes
• Column 1-24 or Row A-P: dispense to the selected
column/row of the current plate.
Disp. Speed Sets speed of all dispensing steps (1 = low, 10 = fast).
Height Sets the height for the dispensing steps.
Advanced
Tip Travel Defines the distance which the tips move during an aspira-
tion, dispense and mix step to artificially track the liquid level,
see 7.8 “Tip travel distances” on page 45
.
• Source Asp: moves the set distance down during aspiration in the source location.
• Source Mix: moves the set distance down and up during
mixing in the source location.
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ASSIST – Operating instructions V09 Programming
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5.5.4 Custom programs
A custom program uses a step based operating technique. Each pipetting step is entered
separately in the sequence in which they will be performed. Custom programs can contain
up to 98 steps.
From the ASSIST Menu select “Custom”, press OK and New . Define and save a name
of your program.
After adding the first step, the selection should now be on the second line. Press OK again
to define the second step. Continue adding steps until your entire pipetting protocol is
defined.The individual steps based upon the following basic operations:
The first line is highlighted. Press OK.
Use the Touch wheel to select a first step from the
menu. Press OK, define the required parameters and
press OK to add the step.
Step Description of Custom Program
Aspirate Sets an aspiration volume, Tip Travel distance (
↓)
1
and pipetting speed.
1. Tip Travel distance: the distance the pipette automatically follows during aspirating, dispensing or mixing allowing to optimize tip immersion depth, see 7.8.
Dispense Sets a dispense volume, Tip Travel distance (
↑)
1
and pipetting speed.
Mix Performs a mixing cycle. Defines the number of cycles, mixing volume, Tip
Travel distance (↕)1 and mix speed.
Purge Purges all remaining liquid currently in the GripTips with the selected
purge speed.
Prompt Pauses the program and displays a message. Three lines with 12
characters each are available. To continue the program, press the RUN
key.
Move X,Z Moves the pipette in Z direction and the plate carrier in X direction to the
selected coordinates. Use the Arrow keys on ASSIST to teach the
position or enter the coordinates manually on the pipette’s screen. To
show the actual coordinates, tap any of the Arrow keys on the ASSIST
device. Click on the pipette to set the coordinates.
Move X The plate carrier travels the set distance in X-direction relative to the
current position by X mm. Setting a negative value (mm) moves it to the
left, setting a positive value (mm) moves it to the right.
The distance from well to well for a 96 well plate is 9 mm and for a 384 well
plate 4.5 mm.
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ASSIST – Operating instructions V09 Programming
26 INTEGRA Biosciences AG
When finished, press to save the Custom program. To run the program, press OK.
Step Description of Custom Program
Move Z Moves the pipette in Z direction to the selected coordinates. Use the
Arrow keys on ASSIST to teach the position or dial the height manually
on the pipette’s screen. Press OK on the pipette to accept the settings.
BlowOut Performs a blow out. A blow out needs to be performed after the last
dispense to remove liquid that may cling to the tips.
Note: When using “Purge” to empty the tips, a blowout/blowin is performed
automatically and does not need to be programmed.
BlowIn After a blow out, a blow in has to follow at some point. It does not have to
follow immediately and can have steps in between. E.g. after the blow out
a move step can be programmed to move the tips out of the liquid, and is
then followed by the blow in.
Delay A delay is a pause between the last and the next step. Define a delay time
(in seconds) or a manual input (pressing the RUN key) to continue.
Loop A loop repeats the steps between the selected step and the loop
command as many times as defined. E.g. if the program reaches the loop
step, it goes back to step 3 and repeats the steps until there 2 times.
The number of program steps can often be shortened by adding a loop.
Call Calls another custom program to run as subprogram in the current
program. This can be utilized to run frequently used sequences, e.g. a tip
touch after a dispense, without programming the individual steps every
time. A custom program can have a maximum of 10 Call steps.
Beep Sets a beep. The sound is only active, if under Preferences - Sounds the
option Messages is set to On.
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ASSIST – Operating instructions V09 Maintenance
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6 Maintenance
WARNING
Always turn off power and disconnect the ASSIST from the mains when
carrying out maintenance work.
6.1 Cleaning
The materials used on the exterior of the ASSIST support regular cleaning intervals. Clean
the external components with a lint-free cloth lightly soaked with mild soap solution in
distilled water or with a 70 % dilution of Isopropyl or Ethanol. Never use acetone or other
solvents.
6.2 Decontamination
From regular use ASSIST should not come into direct contact with liquids. If aerosols or
biohazardous fluids splash on the surfaces, they must be decontaminated in accordance
to good laboratory practice. Wipe the clean surface with a lint-free cloth, lightly soaked e.
g. with the following disinfectants:
• Ethanol 70%
• Microcide SQ 1:64
• Glutaraldehyde solution 4%
• Virkon solution 1-3%
Follow the instructions provided with the disinfectants.
6.3 Servicing
For any service or repairs, please contact your local service technician.
W
ARNING
ASSIST needs to be cleaned before sending it to service. The declaration on
the absence of health hazards must be signed. This is necessary to protect
service personnel.
6.4 Equipment disposal
The ASSIST must not be disposed of with unsorted municipal waste.
Dispose the ASSIST in accordance with the laws and regulations in your area
governing disposal of devices.
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ASSIST – Operating instructions V09 Technical Data
28 INTEGRA Biosciences AG
7 Technical Data
7.1 Environmental conditions
7.2 Specification of the device
7.3 Intellectual Property
The ASSIST is covered under the following patents:
Operation
Temperature range operation 5–40°C
Temperature range storage -10–50 °C
Humidity range Max. rel. humidity 80% for temperatures up to
31°C, decreasing linearly to 50 % rel. humidity at
40°C.
Altitude 0–2000 m AMSL
Pollution degree 2 According to IEC EN/UL 61010-1, i.e. non-conduc-
tive pollution only.
Operation Indoor use only.
Electricity supply Mains adapter input: 100 – 240 VAC, 50/60 Hz
Device input: 22.8–25.2 VDC, 70 W
Dimensions (H x D x W) 400 mm x 360 mm x 340 mm
(with pipette attached: height 510 mm)
Weight 10 kg
Material of surfaces Polyurethane (Housing)
Stainless steel (painted or blank)
Aluminium
Patent Number Country Title Apply to
9,321,048 USA Sample Distribution System And Pro-
cess
ASSIST
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ASSIST – Operating instructions V09 Technical Data
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7.4 Compatible pipettes
The following INTEGRA pipettes are compatible with ASSIST:
7.5 Maximum labware height on plate carrier
Maximum distance between piptte tip end and plate carrier.
Pipette Firmware Serial No.
VIAFLO 3.xx 6xxxxxx
VIAFLO 4.xx 7xxxxxx
Tip type Distance
12.5 µl SHORT 129.9 mm
12.5 µl Standard 121.3 mm
12.5 µl LONG 112.5 mm
50 µl / 125 µl 101.3 mm
300 µl 96.0 mm
1250 µl 54.1 mm
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ASSIST – Operating instructions V09 Technical Data
30 INTEGRA Biosciences AG
7.6 Tip travel distances
The Tip Travel defines the distance which the tips move during an aspiration, dispense
and mix step to artificially track the liquid level. The Tip Travel starts from a user defined
height as shown below.
Section Steps and parameters
Source Source Mix (
↕):
Tip Travel starts from Height (H
s
).
Moves the Source Mix (S
m
)
distance down and up.
Source Asp. (
↓):
Tip Travel starts from Height/Start
Height (H
s
). Moves the Source Asp.
(S
a
) distance down.
Ta rg e t Ta r g e t Disp. (
↑):
Tip Travel starts from
Height (Ht). Moves the
set Target Disp. (Td)
distance up.
Tar g e t M i x (↕):
Tip Travel starts after
dispense from the
Height (H
t
) plus Target
Disp. (Td). Moves the
Tar g e t M i x (Tm)
distance down and up.
Target Asp. (
↓):
Tip Travel starts from
Height (H
t
) plus Ta rget
Disp. (Td) distance.
Moves the Tar g e t
Disp. (T
d
) distance
down.
(Hs)
(Sm)
(Hs)
a
)
(Ht)
(Td)
(Ht)
(T
d
)
(Tm)
(Ht)
(Td)
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ASSIST – Operating instructions V09 Accessories
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8 Accessories
8.1 Accessories for ASSIST
8.2 Accessories for VIAFLO-Pipettens
VIAFLO Electronic Pipettes Part No.
8-channel pipette, 0.5–12.5 μl 4621
8-channel pipette, 2–50 μl 4626
8-channel pipette, 5–125 μl 4622
8-channel pipette, 10–300 μl 4623
8-channel pipette, 50–1250 μl 4624
12-channel pipette, 0.5– 12.5 μl 4631
12-channel pipette, 2– 50 μl 4636
12-channel pipette, 5– 125 μl 4632
12-channel pipette, 10– 300 μl 4633
12-channel pipette, 50– 1250 μl 4634
16-channel pipette, 0.5– 12.5 μl 4641
16-channel pipette, 2– 50 μl 4646
16-channel pipette, 5– 125 μl 4642
Charging options and Bluetooth Part No.
Mains adapter for electronic pipettes 4200
Pipette Li-ion battery 4205
Single pipette charging stand, incl. mains adapter 4210
Charging/communication stand for 1 pipette, incl. mains adapter 4211
Carousel charging stand for 4 pipettes, incl. mains adapter 4215
Linear stand, holds up to 4 charging stations 3215
Mains Adapter for linear stand and carousel charging stand 3216
Charging station for linear stand, incl. connection cable 3217
Charging/communication station for linear stand, incl. connection cable
and USB cable
3218
Bluetooth module for VIAFLO pipettes 4221
Page 32
ASSIST – Operating instructions V09 Accessories
32 INTEGRA Biosciences AG
8.3 Consumables
Reagent Reservoirs Part No.
10 ml Disposable reagent reservoirs, individually sealed,
30 reservoirs per case, sterile
4331
Disposable reagent reservoirs, four sleeves of 50 reservoirs,
200 reservoirs per case, sterile
4332
Reservoir Base, 10 pack 4306
25 ml Disposable reagent reservoirs, individually sealed,
30 reservoirs per case, sterile
4311
Disposable reagent reservoirs, four sleeves of 50 reservoirs,
200 reservoirs per case, sterile
4312
Reservoir Base, 10 pack 4304
100 ml Disposable reagent reservoirs, individually sealed,
30 reservoirs per case, sterile
4321
Disposable reagent reservoirs, four sleeves of 50 reservoirs,
200 reservoirs per case, sterile
4322
Reservoir Base, 10 pack 4305
GripTips for all INTEGRA Pipettes Part No.
Purple
SHORT:
12.5 μl
5 XYZ racks of 384 tips, sterile, filter, SHORT 6475
Purple
LONG:
12.5 μl
Bulk pack, 1 bag of 1000 tips, non-sterile, LONG 4401
5 inserts of 384 tips, non-sterile, LONG, GREEN CHOICE 4402
5 racks of 384 tips, non-sterile, LONG 4403
5 racks of 384 tips, sterile, LONG 4404
5 racks of 384 tips, sterile, filter, LONG 4405
5 inserts of 384 tips, pre-sterilized, LONG, GREEN
CHOICE
4406
Purple
LONG:
12.5 μl
low retention
5 racks of 384 tips, non-sterile, LONG 6503
5 racks of 384 tips, sterile, LONG 6504
5 racks of 384 tips, sterile, filter, LONG 6505
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ASSIST – Operating instructions V09 Accessories
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Purple:
12.5 μl
Bulk pack, 1 bag of 1000 tips, non-sterile 4411
5 inserts of 384 tips, non-sterile, GREEN CHOICE 4412
5 racks of 384 tips, non-sterile 4413
5 racks of 384 tips, sterile 4414
5 racks of 384 tips, sterile, filter 4415
5 inserts of 384 tips, pre-sterilized, GREEN CHOICE 4416
Purple:
12.5 μl
low retention
5 racks of 384 tips, non-sterile 6553
5 racks of 384 tips, sterile 6554
5 racks of 384 tips, sterile, filter 6555
Yel low:
50 μl,
125 μl
Bulk pack, 1 bag of 1000 tips, non-sterile 4421
5 inserts of 384 tips, non-sterile, GREEN CHOICE 4422
5 racks of 384 tips, non-sterile 4423
5 racks of 384 tips, sterile 4424
5 racks of 384 tips, sterile, filter 4425
5 inserts of 384 tips, pre-sterilized, GREEN CHOICE 4426
Yel low:
50 μl,
125 μl
low retention
5 racks of 384 tips, non-sterile 6563
5 racks of 384 tips, sterile 6564
5 racks of 384 tips, sterile, filter 6565
Green:
300 μl
Bulk pack, 1 bag of 1000 tips, non-sterile 4431
5 inserts of 96 tips, non-sterile, GREEN CHOICE 4432
5 racks of 96 tips, non-sterile 4433
5 racks of 96 tips, sterile 4434
5 racks of 96 tips, sterile, filter 4435
5 inserts of 96 tips, pre-sterilized, GREEN CHOICE 4436
Green:
300 μl
low retention
5 racks of 96 tips, non-sterile 6533
5 racks of 96 tips, sterile 6534
5 racks of 96 tips, sterile, filter 6535
Green:
300 μl
wide bore
5 racks of 96 tips, non-sterile 6633
5 racks of 96 tips, sterile 6634
5 racks of 96 tips, sterile, filter 6635
GripTips for all INTEGRA Pipettes Part No.
Page 34
ASSIST – Operating instructions V09 Accessories
34 INTEGRA Biosciences AG
NOTE
Autoclaving of GripTips is not recommended as they may warp during the
process, which can lead to incorrect tip loading and obstruct precise well
targeting.
Blue:
1250 μl
Bulk pack, 1 bag of 500 tips, non-sterile 4441
5 inserts of 96 tips, non-sterile, GREEN CHOICE 4442
5 racks of 96 Tips, non-sterile 4443
5 racks of 96 Tips, sterile 4444
5 racks of 96 Tips, sterile, filter 4445
5 inserts of 96 tips, pre-sterilized, GREEN CHOICE 4446
Blue:
1250 μl
low retention
5 racks of 96 tips, non-sterile 6543
5 racks of 96 tips, sterile 6544
5 racks of 96 tips, sterile, filter 6545
Blue:
1250 μl
wide bore
5 racks of 96 tips, non-sterile 6643
5 racks of 96 tips, sterile 6644
5 racks of 96 tips, sterile, filter 6645
GripTips for all INTEGRA Pipettes Part No.
Page 35
ASSIST – Operating instructions V09
www.integra-biosciences.com 35
Imprint
© 2018 INTEGRA Biosciences AG
All rights to this documentation are reserved. In particular the rights of reproduction,
processing, translation and the form of presentation lie with INTEGRA Biosciences AG.
Neither the complete documentation nor parts thereof may be reproduced in any way, or
stored and processed using electronic media or distributed in any other way without the
written consent of INTEGRA Biosciences AG.
This operating instruction manual has part number 127950, the version is V09. It applies
as of (see Toolbox - Device information):
of ASSIST until a newer revision is released.
VIAFLO, VIALINK, and GripTip are trademarks of INTEGRA Holding, Switzerland.
Manufacturer
Customer service
Please contact your local INTEGRA Biosciences representative.
To find out name and address go to www.integra-biosciences.com
.
Further information and operating instructions in other languages are available on
www.integra-biosciences.com
or on request info@integra-biosciences.com.
FW version VIAFLO Pipettes 4.10 or higher
FW version ASSIST 1.07 or higher
INTEGRA Biosciences AG
CH-7205 Zizers, Switzerland
T +41 81 286 95 30
F +41 81 286 95 33
INTEGRA Biosciences Corp.
Hudson, NH 03051, USA
T +1 603 578 5800
F +1 603 577 5529
info@integra-biosciences.com
www.integra-biosciences.com
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