Integra ASSIST 4500 Operating Instructions Manual
ASSIST Operating instructions
127950_V09
Declaration of conformity
INTEGRA Biosciences AG – 7205 Zizers, Switzerland
declares on its own responsibility that the device
complies with:
Zizers, April 13, 2018
Description Model
ASSIST 4500
EU Directives (DoW: Date of Withdrawal) Before DoW DoW After DoW
Low Voltage Equipment 2006/95/EC 20.04.2016 2014/35/EU
Electromagnetic Compatibility 2004/108/EC 20.04.2016 2014/30/EU
Restriction of Hazardous Substances 2011/65/EU
Waste Electrical and Electronic Equipment 2012/19/EU
Radio Equipment Directive 1999/5/EC 13.06.2016 2014/53/EU
EU Regulations
Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH)
1907/2006
Ecodesign - Power supplies 278/2009
Standards for EU
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
EN 61010-1: 2010
Particular requirements for automatic and semi-automatic
laboratory equipment for analysis and other purposes.
EN 61010-2-81: 2015
Electrical equipment for measurement, control and laboratory
use - EMC requirements.
EN 61326-1: 2013
Standards for Canada and USA
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
CAN/CSA-C22.2
No. 61010-1
Safety requirements for electrical equipment for measurement,
control and laboratory use - General requirements.
UL 61010-1
Particular requirements for automatic and semi-automatic
laboratory equipment for analysis and other purposes.
UL 61010-2-81
Operation is subject to the following two conditions:
(1) this device may not cause harmful interference, and
(2) this device must accept any interference received, including
interference that may cause undesired operation.
Part 15 of the
FCC Rules
Contains FCC ID:
PI4410B
Elmar Morscher
CEO
Thomas Neher
Quality Manager
ASSIST – Operating instructions V09
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Table of Contents
Chapter 1 Introduction
1.1 Symbols used...................................................................................5
1.2 Intended use.....................................................................................6
1.3 Safety notes .....................................................................................7
Chapter 2 Description of the device
2.1 Scope of delivery..............................................................................8
2.2 Overview of the ASSIST...................................................................8
2.2.1 ASSIST device......................................................................8
2.2.2 Keypad..................................................................................8
2.3 Overview of VIAFLO-Pipetten ..........................................................9
2.3.1 VIAFLO pipette parts ............................................................9
2.3.2 Display ..................................................................................9
Chapter 3 Installation
3.1 Operating environment...................................................................10
3.2 Relocating ASSIST.........................................................................10
3.3 Charging the battery of the VIAFLO Electronic Pipette ..................10
3.4 Adapting the pipette holder ............................................................10
3.5 Attaching and removing a VIAFLO Electronic Pipette....................11
3.6 Toolbox settings .............................................................................11
3.6.1 Enabling ASSIST mode ......................................................11
3.6.2 Overview of Toolbox for ASSIST ........................................12
3.6.3 Device Information..............................................................12
3.6.4 Connection between ASSIST and pipette...........................12
3.6.5 Setting tip type ....................................................................12
3.6.6 Adjusting the plate alignment..............................................13
3.6.7 Move Speed........................................................................13
Chapter 4 Operation
4.1 Turn on/off the ASSIST device.......................................................14
4.2 Turn on/off the VIAFLO Electronic Pipette .....................................14
4.3 Bluetooth connection ......................................................................14
4.4 Running a program.........................................................................14
4.5 Pipetting in 96 and 384 well plates.................................................15
4.6 Troubleshooting..............................................................................16
ASSIST – Operating instructions V09
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Chapter 5 Programming
5.1 Overview program categories ........................................................17
5.2 Setting up a program......................................................................18
5.3 Modify existing programs ...............................................................18
5.4 Adjust heights and positions...........................................................19
5.4.1 Enter heights/positions directly ...........................................19
5.4.2 Teach heights/positions on ASSIST ...................................19
5.5 Detailed description of program categories ....................................20
5.5.1 Serial Dilution program .......................................................20
5.5.2 Repeat/Variable dispense programs...................................22
5.5.3 Multi Aspirate program........................................................24
5.5.4 Custom programs ...............................................................25
Chapter 6 Maintenance
6.1 Cleaning .........................................................................................27
6.2 Decontamination ............................................................................27
6.3 Servicing.........................................................................................27
6.4 Equipment disposal ........................................................................27
Chapter 7 Technical Data
7.1 Environmental conditions ...............................................................28
7.2 Specification of the device..............................................................28
7.3 Intellectual Property........................................................................28
7.4 Compatible pipettes........................................................................29
7.5 Maximum labware height on plate carrier ......................................29
7.6 Tip travel distances ........................................................................30
Chapter 8 Accessories
8.1 Accessories for ASSIST .................................................................31
8.2 Accessories for VIAFLO-Pipettens.................................................31
8.3 Consumables .................................................................................32
Imprint ...................................................................................................35
ASSIST – Operating instructions V09 Introduction
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1 Introduction
These operating instructions contain all the information required for installation, operation
and maintenance of the ASSIST. This chapter informs about the symbols used in these
operating instructions, the intended use of the ASSIST and the general safety instructions.
1.1 Symbols used
The operating instructions specifically advise of residual risks with the following symbols:
W
ARNING
This safety symbol warns against hazards that could result in injury. It also
indicates hazards for machinery, materials and the environment.
It is essential that you follow the co rresponding precautions.
C
AUTION
This symbol cautions against potential material damage or the loss of data in
a microprocessor controller. Follow the instructions.
NOTE
This symbol identifies important notes regarding the correct operation of the
device and labor-saving features.
The device is marked with the following symbol:
B
IOHAZARD
The instrument can be potentially biohazardous due to the use of
biozazardous substances by the operator.
ASSIST – Operating instructions V09 Introduction
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1.2 Intended use
This is a general-purpose laboratory instrument for use in research only. Any use of this
instrument in a medical or IVD setting is the sole responsibility of the user.
If the ASSIST is used in a manner not specified by INTEGRA Biosciences, the protection
provided by the ASSIST may be impaired.
With a VIAFLO electronic multichannel pipette attached, ASSIST performs pipetting
operations in microplates automatically.
N
OTE
Only VIAFLO multichannel pipettes (as of serial number 600 000 and
firmware version 3.00 or higher) can be used with ASSIST. The VIAFLO
pipette needs its own Bluetooth module, which has to be ordered separately
(part no. 4221).
VIAFLO Electronic Pipettes are microprocessor controlled and stepper motor driven
pipettes. They are used for aspirating and dispensing liquids in the volume range of 0.5–
1250 µl using GripTip pipette tips. Please refer to the VIAFLO Electronic Pipettes
operating instructions for more detailed informations on www.integra-biosciences.com
.
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1.3 Safety notes
ASSIST complies to the recognized safety regulations and is safe to operate. ASSIST can
only be operated when in intact condition and while observing these operating
instructions.
The device may be associated with residual risks if it is used or operated improperly by
untrained personnel. Any person operating the ASSIST must have read and understood
these operating instructions, and particularly, the safety notes, or must have been
instructed by supervisors so that safe operation of the device is guaranteed.
C
AUTION
Do not open or modify the ASSIST in any way. Repairs may only be
performed by INTEGRA Biosciences AG or by an authorized after-sales
service member. Parts may be replaced with original INTEGRA Biosciences
parts only.
W
ARNING
Do not use the ASSIST near flammable material or in explosive areas. Also,
do not pipette highly flammable liquids such as acetone or ether.
When handling dangerous substances, comply with the material safety data
sheet (MSDS) and with all safety guidelines such as the use of protective
clothing and safety goggles.
N
OTE
Prolonged exposure of the ASSIST to UV-light can cause discoloration and/
or yellowing. However, this will not affect the performance of the device in
any way.
Regardless of the listed safety notes, additional applicable regulations and guidelines of
trade associations, health authorities, trade supervisory offices, etc. must be observed.
Please visit our website www.integra-biosciences.com
on a regular basis for up to date
information regarding REACH classified chemicals contained in our products.
ASSIST – Operating instructions V09 Description of the device
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2 Description of the device
2.1 Scope of delivery
• ASSIST (VIAFLO-Pipetten to be ordered separately)
• Mains adapter
• Sample pack 10 ml, 25 ml and 100 ml reagent reservoir
C
AUTION
Verify the scope of delivery when unpacking the device and check for
potential transportation damage. Do not operate a device that is dama ged,
instead contact your local dealer.
2.2 Overview of the ASSIST
2.2.1 ASSIST device
2.2.2 Keypad
1 Tower with pipette arm. Arm moves in Z-direction
2 Plate carrier, moves in X-direction
3 Power connection
4 USB port, for firmware updates
5 Keypad
6 Reservoir position
7 Instrument base
8 Pipette holder on pipette arm with lever to shift the
pipette in Y-direction
9 Gripper, lever to release the pipette
10 VIAFLO Electronic Pipette
11 Up and Down arrow keys move the pipette
along the Z-axis to teach pipetting heights.
12 Left and Right arrow keys move the plate
carrier along the X-axis to adjust the plate carrier
position.
13 Start/Pause key, to start/stop operations.
Green LED blinks: press to start operation/homing
Green LED lights: operation performed
14 Bluetooth control LED (lights blue if connection is
active) and Error LED (flashes red in case of error)
15 On/Off key (lights green if On)
3
9
5
6
8
2
10
1
7
4
11
12
13
14
15
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2.3 Overview of VIAFLO-Pipetten
2.3.1 VIAFLO pipette parts
2.3.2 Display
The Display shows all pipetting options.
16 Display
17 Back button, to navigate backward
18 Touch wheel, spin to scroll and move the cursor
19 OK button, to make a selection
20 Left and right arrow buttons, for selections
21 Purge button, to empty tips
22 Run key, to start operations
23 Tip ejector
24 Finger hook, facilitates easy operation
25 Volume indicator label, color matches GripTip box
insert.
Battery life indicator
Instruction
Volume
Options
16
17
20
21
22
23
18
19
24
25
Current category
Active step
OK - Select
a program
category and
a program
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3 Installation
3.1 Operating environment
ASSIST has been designed for use in a laboratory. It shall be operated in a dry and dustfree location with a temperature of 5–40°C and a maximal (non-condensing) relative
humidity of 80 %, see “7.1
Environmental conditions” on page 28.
3.2 Relocating ASSIST
Before ASSIST can be relocated, clear the Instrument base (7). Swi tch off the devi c e and
disconnect it from the electricity mains. Lift the ASSIST on both sides of the Instrument
base. Never lift the instrument on the Tower.
3.3 Charging the battery of the VIAFLO Electronic Pipette
The battery indicator in the upper right corner of the pipette screen informs about the
battery status. When it turns red, the pipette needs to be charged.
C
AUTION
Use only the approved INTEGRA battery, power supply or charging stand.
Use of an incompatible power transformer can damage the pipette.
The battery can be charged using either the mains adapter or a charging stand, see (“8.2
Accessories for VIAFLO-Pipettens” on page 31).
3.4 Adapting the pipette holder
The pipette holder can be adapted to the type of the VIAFLO pipette.
Insert the mains adapter connector into the receptacle on the top
back of the pipette. Plug the mains adapter into a wall outlet.
To adapt the holder for an 8- or 16-channel pipette, pull the silver
lever down as shown beside.
Lift the lever up to insert a 12-channel pipette.
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3.5 Attaching and removing a VIAFLO Electronic Pipette
Rotate the lower hosing of the VIAFLO Electronic Pipette by 90 degrees as displayed in
the picture.
3.6 Toolbox settings
3.6.1 Enabling ASSIST mode
The ASSIST mode is hidden from the Main Menu of the VIAFLO pipettes by default.
To install a pipette, hold it at an angle and insert it into the pipette
holder.
Then lift up until the black gripper snaps in place.
To release the VIAFLO Electronic Pipette lift the black gripper by
pushing down on one end.
Use the Touch w h e e l to selec t Toolbox from the Main Me nu and p ress
OK.
Select Preferences and then Main Menu. Enable the ASSIST mode by
pressing OK (green ) and press to save your settings.
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