Digital Sonifier
®
Models 250 & 450
User’s Manual
EDP 100-214-239
Rev. A
BRANSON Ultrasonics Corporation
41 Eagle Road
Danbury, Connecticut 06813-1961 U.S.A.
(203) 796-0400
EDP 100-214-239 Rev. A February 2001
Manual Change Information
At Branson, we strive to maintain our position as the leader in ultrasonics plastics joining,
cleaning and related technologies by continually improving the designs, circuits and compo-
nents in our equipment. These improvements are incorporated as soon as they are developed
and thoroughly tested.
Information concerning any improvements will be added to the appropriate technical docu-
mentation at its next revision and printing. Therefore, when requesting service assistance for
specific units, note the Release information found on the cover of this document, and refer to
the printing date which appears in the lower right corner of this page.
Patents and Copyright
Copyright ©1998 by Branson Ultrasonics Corporation, Danbury, Connecticut, U.S.A.
All rights reserved.
Manual part number: EDP 100-214-239 Rev. A, printed February 2001.
Branson Ultrasonics Corporation equipment is manufactured under one or more of the fol-
lowing U. S. Patents:
4,249,986; 4,315,181; 4,363,992; 4,551,690; 4,651,043; 4,786,356; 4,973,876; 5,095,188;
5,435,863.
Note: Sonifier® is a registered trademark of Branson Ultrasonics Corporation, Danbury,
Connecticut, U.S.A. Other trademarks and service marks respectfully mentioned herein are
held by their respective owners.
Digital Sonifier
®
User’s Manual
Forward
Congratulations on your choice of a Branson Ultrasonics Corporation system!
The Branson Digital Sonifier
®
system is the new e st gene ra ti o n of prod uc t using this soph ist i-
cated technology for a variety of customer applications. This manual should be kept with the
system.
Thank you for ch oo si n g a Branson product !
Introduction to this manual
This manual is arranged into seven structured chapters which will help you find the informa-
tion you may need to know to safely handle, install, set up, program, operate, and/or maintain
this product. Additional information is provided in several Appendix chapters.
In the event you require additional assistance or information, please contact our Product Sup-
port department (see
’How to Contact Branson’
on page 1-5 for information on how to contact
us) or your local Branson representative.
100-214-239 Rev. A
For Your Notes
100-214-239 Rev. A i-5
Digital Sonifier
100-214-239 1-1
Safety Considerations
Digital Sonifier
1 Safety and Support
1.1 Safety Considerations --------------------1-1
1.2 General Precautions ---------------------1-2
1.3 Regulatory Compliance--------------------1-3
1.4 Warranty ---------------------------1-4
1.5 How to Co ntact Branson -------------------1-5
1.1 Safety Considerations
CAUTION
j The following safety considerations should be observed when operating t he
Digital Sonifier®:
• Make s ure that the equipm ent is properly grounded. DO NOT operate if it is not.
• The unit is equi pped with a three-condu ctor cord and three-prong grounding-type plug,
and must be plugged into a three-prong grounding-type wa ll recep tacle. DO NOT under
any circumstances remove the power cord ground pron g.
• DO NOT operate the equipment with the cover removed. High voltage is present within
the eq uipm ent wh en connec ted to plant wiring.
• DO NOT turn on the ultrasonics without the converter and horn attached.
• DO NOT touch the horn or tip when ultrasonics are active. When handling, removing, or
attaching a horn or t ip, be sure that the ON/OFF s witch is set to O FF.Touching the horn
or tip while the unit is on can result in seriou s personal injury.
• DO NOT a llow the horn or microtip to contact lab stands, beakers, or similar objects.
Horn/microtip failure may result.
• DO NOT o perate the equipment at more than 70% amplitude when using a microtip
horn.
• Establish a standard of operation and test equipment periodically.
1-2 100-214-239
Safety and Support
1.2 General Precaution s
1.2.1 Intended Use of the System
The 250/450 Digital Sonifiers can be used to disrupt cells, bacteria, spores, or tissue, and are ideal for initiating and accelerating chemical, biochemical, and physical reactions, and for de-gassing liquids. With the Digital Sonifier, you can prepare
an emulsion to 0.01 micron, homogenize immiscible liquids, polymerize some
materials, and depolymerize others.
1.2.2 Safety Measures and Guards
This manual contains operation instructions for the Digital Sonifier cell disruptor
Models 250 and 450. It contains information essential to the proper use and care
of this equipment. The manual contains notes, warnings, and cautions. These are
described as follows:
NOTE
i Provides information that the reader should follo w to prevent inconvenience.
CAUTION
j Advises the user of a hazard that can cause equipment damage or personal
injury.
WARNING
j Alerts the reader to a hazard that can result in severe personal injury.
Do NOT disregard a WARNING.
1.2.3 Safe Operation
Setup and Operation instructions are found in Chapter 6 of this manual.
For safe operation, please ensure that all people using this equipment follow those
instructions and observe all CAUTION and WARNING notices.
1. Make s ure th at the equipment is properly grounded. DO NOT operate if it is not.
100-214-239 1-3
General Precau tion s
Digital Sonifier
2. The unit is equipped with a three-conductor cord and three-prong grounding type
plug and must be pl ugged into a three-prong grounded receptacle. DO NOT under
any circumstances remove the power cord ground prong, or use a plug ‘Adapte r’.
3. DO NOToperateequipmentwiththecoverremoved. Highvoltage is present within
the equipment when connected to plant wi ring.
4. Do not al low the horn or microtip to contact lab stands, beakers, etc. or horn/
microtip failure may result.
5. Establish a standard of operation and periodically t es t the equipment , as described
in Ultrasonic Test on page 4-17.
Although the Digital Sonifier operates outside the normalrange of human hearing,
some applications can create audible noise above 85dB. Noise can occur, for
example, when the device touches a solid material, or when its operation causes
nearby objects in the lab to vibrate. If an uncomfortable level of noise is present,
the operator should wear ear protection for safe operation.
Appropriate eye protection should be worn when operating the Digital Sonifier, to
prevent possible splash injury originating in the solution.
WARNING
j
Never touch the horn or tip when ultrasonics are active. Touching the horn or
tip while the unit is on can result in serious injury. When you handle, remove,
or attach a horn or tip, always make sure that the ON/OFF switch is OFF.
1.2.4 Setting Up the Workplace
The unit should be positioned away from radiators and heating vents. A fan inside
theunit maintains a safe operating temperature in the power supplyby circulating
air over the components. Therefore, place the unit so that the air intake on the bottom of the power supply is not blocked. Periodically, unplug the unit and clean the
air intake underneath the power supply to ensure that dust or dirt is not restricting
the flow of air. If the Digital Sonifier is to be used for remote operation, ensure that
the unit is situated within full view of the operator, to prevent injury or equipment
damage through an accidental or automatic start-up.
1.3 Regulatory Compliance
The Digital Sonifier is designed for compliance withthe following regulatory guidelines.
• European norms EN-61010-1, EN55011, EN50082-1, ENV50140, ENV50141
• IEC 529 (IP-65 water resistant for Membrane Keypad)
The Digital Sonifier is CE compliant.
1-4 100-214-239
Safety and Support
1.3 Warranty
Refer to the “Terms and Conditions of Sale” found on the back of your Invoice for
information about the product Warranty issued of your Branson products. If you
have any questions, please contact your Branson representative. The product warranty information is summarized below.
WARRANTY
When used in accordance with written instructions and under normal operating
conditions, Branson Ultrasonics Corp. equipment is guaranteed to be free of
defects in MATERIAL and WORKMANSHIP for two (2) years from the date of original delivery by BRANSON or by an authorized representative. Any unit which
proves defective during the stated period will be repaired free of charge or replaced
at the sole discretion of Branson Ultrasonic S.A., 9, ch. Faubourg-de-Cruseilles,
CH-1227 Carouge/Geneva or an authorized repair station as advised by BRANSON,providedthedefectiveunitis returnedproperlypackedw ith alltransportation
charges prepaid. A limited warranty as specified may apply to certain components
of the equipment.
WARRANTY EXCEPTIONS
This warranty shall not apply to equipment subjected to misuse, improper installation, alteration, neglect, accident or improper repair.
This warranty is limited to the original purchaser and is not transferable.
Horns and tips fabricated by Branson for use in equipment described in this manual
are manufactured to exacting parameters. Using altered or modified horns and tips
or horns and tips otherwise unqualified by Branson can produce undue stresses
that may damage the equipment. Equipment failures resulting from using unqualified horns and tips are not covered by the Branson warranty.
Microtips are designed to give maximum mechanical energy output. Since they
operate close to the stress limits of titanium, Branson cannot guarantee microtips
against failure.
CONTA CT Y O UR B RAN SON RE PRESENTATIVE OR BRANSON ULTRASONICS CORPORATION, DANBURY, CONNECTICUT, SHOULD Y OU HAVE ANY
QUESTIONS CONCERNING HORN QUALIFICATION.
100-214-239 1-5
How to Contact Branson
Digital Sonifier
1.4 How to Contact Branson
The mailing address and telephone information for Branson is as follows:
Branson Ultrasonic SA
9, chemin Faubourg-de-Cruseilles / P.O. Box 1031
CH-1227 Carouge/Geneva
Phone : +41-22-304 83 58 Fax: +41-22-304 83 59
Tell the operator which product you have and which person or department you
need.If after hours,please leave a voice messagewith your name and return telephone number.
1.4.1 Before Calling Branson for Assistance
This manual provides information for troubleshooting and resolving problems that
couldoccur with the equipment (see Chapter 7). If you still require assistance,
Branson Product Support is here to help you. The following questionnaire lists the
common questions you will b e asked when you conta ct the Product Support
department, to help identify the problem.
Before c alling, determine the following information:
1. Your company name and location .
2. Your return telephone number.
3. Have your manual with you. If troubleshooting a problem, refer to Chapter 7.
4. Know your equipment model and serial num bers (found on a gray data label on the
units). Information about the Horn (part number, gain, etc.) or other toolin g may be etched
into the tooling. Software- or-firmware based systems may provide a BOS or software versionnumber,w hich may be required. (The Digital Sonifierprovides the firmware information
on the start-up screen.)
5. What tooling (horn) and booster are being used?
6. What are the setup parameters and mode?
7. Is your equipment in an automated system? If so, what i s supplying the “start” signal?
8. Describe the problem; provide as much detail as possible. For example, is the problem
intermittent? H ow o ften does it occur? How long before it occurs if you are just powering
up? If an error is occurring, which error (give error number or name)?
9. List the steps you have already taken.
10. What is your application, including the material s being processed?
11. Have a list of service or spare parts you have on hand (tips, horns, etc.)
1-6 100-214-239
Safety and Support
Returning Equipment for Repair
Before sending equipment for repair, provide as much information with the equipment to help determine the problem with the system. Fill in any details below or on
a separate sheet.
1. Describe the problem; provide as much detail as possible. For example, is this a
new problem? Is the problem intermittent? How often does it occur? H ow long
before it occurs if you are just powering up?
________________________________________________________________
________________________________________________________________
________________________________________________________________
2. Is y our equipm ent i n an automat ed s ystem? If so, is the problem related to Start/
Stop control, or interaction with P LC’s or other devices, etc.?
3. If the problem is with an external signal or output, which one?
If known, include plug/pin # (e.g., P29, pin #3):
__________________________________
4. What are the setu p parameters?
________________________________________________________________
________________________________________________________________
5. What is your application (e.g., cont inuous, pulse, temperature, etc.)?
________________________________________________________________
6. Name and phone number of the person most familiar with the problem:
________________________________________________________________
7. Notify th e Branson office prior to shipping the equipment.
NOTE
i ToReturn Equipment to Branson, you must firstobtain an RGA number from
a Branson representative, or the shipment may b e delayed or refused.
8. For equipment not cov ered by warranty, include a purchase order for the repair
costs to avoid delay.
9. Pack carefully in original packing mate rial to avoid damage in shipment.
10. Return general repairs by any desired method. Send priority repairs by air freight.
11. Prepay the transportation charges FOB Danbury, Connec ticut, U.S.A.
Notes:
________________________________________________________________
________________________________________________________________
________________________________________________________________
100-214-239 1-7
How to Contact Branson
Digital Sonifier
Returning Equipment for Repair (to Geneva facility)
NOTE
i To return equipment to Branson, you must first obtain an RGA number from
a Branson representative, or the shipment may b e delayed or refused.
If you are returning equipment to Branson for repair,you must first call the Danbury
Repair department to obtain a Returned Goods Authorization
(RGA)number.(If
you request it, the repair department will fax a Returned Goods Authorization form
to fill out and return with your equipment.)
Branson Ultrasonic SA
9, chemin Faubourg-de-Cruseilles / P.O. Box 1031
CH-1227 Carouge/Geneva
Phone : +41-22-304 83 58 Fax: +41-22-304 83 59
• Provide as much information as possible that wil l help identify the need for repair.
• Carefully pack the equipment in original packing cartons.
• Clearly label all shipping cartons w ith the RGA number on the outside of cartons as well
as on your packing slip, along with the reason for return.
• Return general repairs by any convenient method. Send prio rity repairs by air freight.
• You must prepay the transportation charges FOB Danbury, Connecticut, U.S.A.
Obtaining Replacement Parts
You can reach Branson Parts Store at the following telephone numbers:
Branson Part Store
direct telephone number: +41-22-304 83 58
fax number: +41-22-304 83 59
Many parts can be shipped the same day if ordered before 2:30 p.m., Eastern time.
A parts list is found in Section 7 of this manual, listing descriptions and EDP part
numbers. If you need replacement parts,coordinate the following with your purchasing agent:
• Purchase order number
• ‘Ship t o’ information
• ‘Bill to’ information
• Shipping instructions (air fr eight, truck, etc.)
• Any special i ns truct ions (for examp le, “Hold at the airport and call”). Be sure to give a
name and phone number
• Contact name information
1-8 100-214-239
Safety and Support
For Your Notes
100-214-239 Rev. A 2-1
Overview o f Digital Sonifier
Digital Sonifier
2 Introduction to the Digital Sonifier
2.1 Overview of Digital S onifier------------------2-1
2.2 Controls and Commands -------------------2-3
2.3 System Features -----------------------2-9
2.1 Overview of Digital Sonifier
The 250/450 Digital Sonifier is often used by laboratory personnel in the medical and chemical
process fields. The system consists of three core elements: the power supply, the controls, and
the converter and horn. The system can interface with customer equipment including a remote
computer or terminal, dot matrix printer, temperature probe, and User I/O.
Figure 2.1 Digital Sonifier
Power Switch
Amplitude Control
Front
Panel
Keypad
Bargraph
LCD Display
Select keys
and
Arrow keys
2-2 100-214-239 Rev. A
Introduction to the Digital Sonifier
The 250 and 450 Digital Sonifiers differ only in their output power ratings, measured when the
output control is at maximum setting. The 250’s maximum available output power is up to 200
watts; the 450’s maximum available output power is up to 400 watts.
The digital controls on the Digital Sonifier allow for accuracy and repeatability of control set-
tings. You enter application parameters into the system either through the keypad on the front
panel of the unit, or remotely through a computer keyboard, and can save up to twenty (20)
sets of preset control parameters. You can view operating parameters through the twenty-char-
acter, four-line LCD display on the unit control panel and on a remote terminal if one is con-
nected. At System Setup, you can select a language for the display: English, German, French,
Italian, or Spanish.
The Digital Sonifier’s power supply converts AC line voltage to 20kHz electrical energy. This
high-frequency electrical energy is fed to a converter where it is converted to mechanical vibra-
tions. The heart of the converter is a lead zirconate titanate electrostrictive element which,
when subjected to an alternating voltage, expands and contracts. The converter vibrates in a
longitudinal direction and transmits this motion to the horn tip immersed in the solution,
which causes cavitation. The implosion of microscopic cavities in the solution results, causing
the molecules in the medium to become intensely agitated. The ultrasonic vibrations transmit-
ted through the horn can also be applied directly to a solid workpiece, such as tissue, through
a variety of different tips that can be attached to the horn.
The Digital Sonifier is a constant amplitude device. As the load or pressure on the horn face
increases, the power supply develops more power to maintain the amplitude for any given out-
put control setting. When the horn is operated in air, it is subjected to minimum pressure, and
minimum power is required to maintain amplitude.
The load increases when the horn is immersed in a liquid; the more viscous the liquid, the
higher the load and the more power developed. If a flow-through cell that can be pressurized
is used, thereby increasing pressure on the horn, even more power is developed. For any given
application, more power results when a horn of higher amplitude or larger radiating surface is
used, or when any horn is driven at higher amplitude by increasing the amplitude.
By setting various operation parameters, you can precisely control the way in which ultrasonics
are applied to the sample. You can:
• Specify the time duration of the experiment
• Adjust the amplitude setting between 10% and 100% of maximum amplitude
(microtip 70% maximum)
• Prevent excessive temperature increase in the sample by setting ultrasonics to
operate in Pulse m ode or in Pulse/Pause mode
• Bring a sample to a desired temperature an d hold it there, v ary ing by only a few
degrees, for a d es ired duration using the Pulse/Paus e mode
• Set the maximum allowable temperature in t he sample, so that ultrasonics will
stop automatically when the specified temperature is reac hed
100-214-239 Rev. A 2-3
Controls and Commands
Digital Sonifier
2.2 Controls and Commands
This section describes the controls and commands that you use to operate the Digital Sonifier.
A detailed description of how and when to use each front panel control, the valid formats for
the data that you enter, and the response you receive from the system when you use each of
these controls is provided in
Chapter 6, Operations
.
The Digital Sonifier is equipped with a keypad and LCD display on the front panel of the unit.
With the keypad, you can set functional modes of operation and input digital parameters. Avail-
ability of the various functions depends on the mode or state of the system. If you attempt to
use a function that is not available, you will be alerted by a beep.
Figure 2.2 Digital Sonifier front-panel Controls
MODEL 450 SONIFIER
COPYRIGHT 1997
Digital Sonifier
®
2-4 100-214-239 Rev. A
Introduction to the Digital Sonifier
Operation of the Digital Sonifier can also be controlled through a remote computer terminal or User I/O
device. You always have the ability to halt operation, whether control is direct or remote.
Each key on the front panel of the unit, except for the numeric keys, Print and Test, has an LED indicator near
it. When the key is active, the LED is turned on. Some of the keys on the Digital Sonifier keypad have equivalent
commands (preceded by !) that can be entered through the remote terminal’s keyboard.
• Section 2.2.1, Digital S onifier Keypad Description, beg inning on page 2-4, describes each key on the
Digital Sonifier keypad and identifies its equivalent remote terminal command (preceded by an ! exclamation point) if applicable.
• Section 2.2.2, R emote Terminal Commands, beginning on page 2-7, provides a detailed desc ript ion of
all remote terminal commands. S ec ti on 2.2.3, Back Panel Connect ions, on page 2-8, d es cribes the back
panel of the unit.
2.2.1 Digital Sonifier Keypad Description
LCD Display
The front panel is equipped with a four-line, 20-character display screen where menus, parameter options, mes-
sages, and warnings are displayed. If the data being displayed has more than one page, you can navigate up and
down in the display by pressing the Page Up and Page Down arrow keys.
The f irst page of a typical Ready display.
If you wish to see the second page, press
the Down Arrow button.
The s ec ond page of a Ready display.
If y ou w ish t o see the first page, press the
Up A rrow button.
PRESET ID#16
TIME= 0:12:52 hr/m/s
MAX. TEMP= 104.3
°F
AMPLITUDE= 43 %
PULSE ON= 2.1 SEC
PULSE OFF= 1.6 SEC
PULSE TEMP= ___._ F
PROBE TEMP= 67.6
°C
100-214-239 Rev. A 2-5
Controls and Commands
Digital Sonifier
Line Select Keys
There are four Line Select keys to the right of the display. When a Line Select key is active, the
LED next to it is turned on. You can press a Line Select key to:
• Select a line in the Parameter Entry menu display for a parameter entry.
• Toggle values in a parameter or System Set up sub menus (for exam ple, Yes or
No).
• Select an item from the options available when the system issues a warning/
error mes s age.
Once a Line Select key is pressed, its LED is turned off. When you begin entering a value for
a parameter you selected with a Line Select key, all the other Line Select keys become inactive
and their LEDs are also turned off until you complete the parameter entry. If you have selected
a line but have not begun to enter a value, the other Line Select keys remain active, and you can
select another line on which to enter a parameter value.
Page Up and Page Down Keys
The Page Up and Page Down arrow keys are active when you are displaying a menu that has
more than one page. You can use the Page Up and Page Down keys to move up and down in
the menu. If there are more menu items above the page you are viewing, the Page Up arrow is
active and its LED is turned on. If there are menu items below the page you are viewing, the
Page Down arrow is active and its LED is turned on.
Start/Stop Key (!ON and !OFF Commands)
The Start/Stop key and !ON command are active during Parameter Entry mode (except when
you are changing a parameter value) and during the Sonics Active displays. When you press the
Start/Stop key or type in the !ON Command, the experiment is executed, and the LED next
to the key is turned on. When you press the Start/Stop key again or type in the !OFF com-
mand, the experiment is stopped, and the LED is turned off.
Setup Key
The Setup key is active during Parameter Entry mode (except while you are changing a param-
eter value) and while you are viewing the Setup Menu. When you press the Setup key, the first
page of the Setup menu is displayed and the Setup key’s LED is turned on. When you press
the Setup key again, the Setup menu is closed and its LED is turned off.
Test Key (!TS Command)
The Test key and the !TS command are active when the system is in the Ready state. When you
press the Test key or type in the !TS command, ultrasonics are enabled for two seconds. To
turn on ultrasonics for another two seconds, you can press the Test key or type in the !TS com-
mand again.
2-6 100-214-239 Rev. A
Introduction to the Digital Sonifier
Pause Key (!H Command)
The Pause key and the !H command are active only when the unit is operating. When you press
the Pause key or type in the !H command, operation of the unit halts and the Pause key’s LED
is turned on. When you press the Pause key or type in the !H command again, the unit resumes
operation, and the LED is turned off.
Recall Key (!R Command)
The Recall key and the !R command are active only during Parameter Entry mode, except when
you are changing a parameter value. When you press the Recall key or type in the !R command,
a message is displayed and the LED is turned on. Refer to
Section 6.6, Save and Recall Presets,
on
page 6-40, for information on how to save and recall presets.
Save Key (!SV Command)
The Save key and the !SV command are active during Parameter Entry mode, except when you
are changing a parameter value. When you press the Save key or type in the !SV command, a
message is displayed and the LED is turned on. Refer to Section
Section 6.6, Save and Recall Pre-
sets,
on page 6-40, for information on how to save and recall presets.
Print/Send Key (!P and !S Commands)
The Print/Send key and the !P and !S commands are only active after a test or experiment has
been executed, and before another test or experiment begins or the Setup menu parameters are
changed. For this function to operate, a printer, a remote terminal, or both must be enabled.
When you press the Print/Send key, report data is sent to the remote terminal and to the
printer. Refer to
Section 6.7, Printing/Sending Reports,
on page 6-41, for a description of the data
that is sent to the terminal and printer.
Enter Key
You can press the Enter key on the Digital Sonifier or on the computer keyboard to:
• Cause the system to accept a modified parameter value
• Clear an error/warning message.
• Store a Setup parameter value
If you have entered a modified parameter value, the system checks the value for validity and
format. If the v alue you entered is valid, the value is accepted, and it replaces the old value. The
original value is lost and cannot be recovered unless it has been stored under a preset ID num-
ber. If the modified value is invalid, an appropriate error message is displayed.
100-214-239 Rev. A 2-7
Controls and Commands
Digital Sonifier
Clear Key
The Clear key on the Digital Sonifier is active at any time when a parameter can be changed.
If you press the Clear key when you are modifying a parameter value, the entry field becomes
blank, and the cursor is moved to the least significant position. If you press the Enter key while
the entry field is blank, no value is stored for the parameter.
Numeric Keys
The keys for 1 through 9 and 0 are active at any time when a parameter has been selected to
be changed.
2.2.2 Remote Terminal Commands
This table describes the remote terminal commands and their functions. All of the remote ter-
minal commands must be preceded by an exclamation point. Refer to
Chapter 6, Operations
, for
a description of the system’s response to each of these commands and the actions you should
take at each response.
Ta ble 2-1: Remote Terminal Commands
Send this
command...
When you want to...
!T Enter the TIME duration val ue.
!MT Enter th e MAX IMUM T EMPERATURE value.
!PO Enter the PULS E O N time value.
!POF Enter the P U LSE OFF time value.
!PPT Enter the Pulse Temperature value.
!S Send all report data to the remote terminal.
!P Print all report data, if a printer is enable d.
!R Recall a particular preset.
!H
Pause operation of the Digital Sonifier until you send another !H
command.
!MT Enter the M ax im um Temperature value.
!TS Test the system for two seconds.
!ON Start the experiment.
!OFF Stop the experiment.
!KL
Lock out the keypad on the front panel. The keypad is enabled
again if you send another !KL command, or if you turn the power off
on the Dig ital Sonifier and then turn it on.
!SV S av e the parameters entered.
!RP View all the test or experiment paramet er values.
2-8 100-214-239 Rev. A
Introduction to the Digital Sonifier
2.2.3 Back Panel Connections
Figure 2.3 Back Panel of Digital Sonifier
The back panel of the Digital Sonifier is equipped with:
• An IE C -type power cord connec tion for connecting the power suppl y to a
grounded electrical outlet.
• A fuse holder for access to a replaceable 5x20mm protective fuse. The fuse is
a glass slow-blow type (refer to the data tag for the fuse rating).
• A converter cable with connector to connect the power supply to the Converter.
• An RS-232 connector (female DB9) for connecting a remote comp uter terminal
to the unit. This connection requires a cable w ith a 9-pin male connector.Serial
ASCII data is transferred through this connection at a user-selectable baud rate
of 1200, 2400, 9600, or 19200 bps with 8 databits, 1 stop bit, and no parity.RX,
TX, and Signal Ground are supported.
• A parallel printer connector (female DB25) re quiring a 25-pin male connector.
During System Setup, you can select the dot-matrix printer choices from Panasonic, E pson, or IBM .
• A User I/O connector (female DB15) requires a cable with a 15-pin male DB15
connector. The User I/O provides proprietary system control signals
• A 1/4-inch phone-jack s t yle con nec tor for an optional temperature probe.
IEC-type power
cordset connector
Temperature P robe
connector
Remote
Terminal
Parallel
Printer
User I/O
connector
Fan
Fuse holder
Converter cable
and connector
(RS-232)
100-214-239 Rev. A 2-9
System F eatures
Digital Sonifier
2.3 System Features
The Digital Sonifier includes the following features:
• Front-panel system O n/O ff switch
• Separate fron t-panel Stop and Paus e buttons f or experiment c ycle s
• Power output of up to 200 watts (Model 250) an d up to 400 watts (Model 450)
• Nominal 117 Volt ( North American) and 200-245 Volt (export) models available (factory
set)
• Multiple languages for display messages: English, Spanish, Italian, German or French.
• Front-panel LCD display, showing parameter sett ings, alarms and messages
• Membrane front panel with num eric keypad for parameter entry and function selection
• Digital parameter setting, with valid parameter range checking
• Twenty (20) presets for different parameter Setups
• Digital Wattmeter
• Front panel Bargrap h display of relative power while running
• Amplitude control, analog, adjustable while running
• Pulse or Continuous operation
• Temperatu re option
• Sample Hold using Pulse/Pause operation
• Digital Timer (9 hours, 59 minutes, 59 seconds)
• Automatic end-of-cycle using Limits
• Factory-tuned 20 kHz u ltrason ic design, requires no user adjustment
• Parallel Printer interface
• Printed Report capability, with date stamp and parameter information
• Serial interfa ce (for remote computer or terminal)
• User I/O, offers remote start/stop control
For additional feature information, please contact your Branson representative.
2-10 100-214-239 R ev. A
Introduction to the Digital Sonifier
For Your Notes
100-214-239 Rev. A 3-1
Delivery and Handling
Digital Sonifier
3 Delivery and Handling
3.1 Delivery and Handling
The Digital Sonifier has no special handling constraints. On receipt of your Digital Sonifier sys-
tem, take the following steps:
1. Inspect the carton for signs of damage.
2. Open the carton and locate the packing list.
3. Carefully unpack t he components and check them against the packing list.
4. Save all packing materials in case the equipment needs to be shipped.
5. Inspect the components for any damage that may have oc curred during s hipping.
Report all shipping damage to your carrier.
3-2 100-214-239 Rev. A
Delivery and Handling
For Your Notes
100-214-239 Rev. A 4-1
Installation Chec klist
Digital Sonifier
4InstallationandSetup
4.1 Installation Checklist ---------------------4-1
4.2 System Block Diagram --------------------4-2
4.3 System Component Description ---------------4-2
4.4 Assembling the Equipment ------------------4-9
4.5 Input Power Requirements ------------------4-13
4.6 Electrical Connections to Equipment-------------4-13
4.7 Guards and SafetyEquipment ----------------4-17
4.8 Ultrasonic Test ------------------------4-18
4.1 Installation Checklist
To install the Digital Sonifier, you will need these items:
• Digital So nif ier unit
• Power cordset
• Converter
• Horn (and horn tips, if applicable)
• Spanner wrench (can be ordered from Branson; refer to Appendix C.)
• Open-ended wrench (can be ordered from Branson; refer to Appendix C.)
• Any acc es s ories shipped with the unit
• Any customer-supplied items, (i.e., c omputer, printer, and temperature probe)
Refer to
Appendix B, Optional Items Parts List
, for specific parts required for the optional items,
and to
Appendix C, Accessories Parts List
, for parts needed for the available accessories.
4-2 100-214-239 Rev. A
Installation and Setup
4.2 System Block Diagram
The block diagram shows the relative interaction of the components of a Digital Sonifier sys-
tem. The items inside the bold outline are found in the Digital Sonifier unit. Some elements
shown are optional.
4.3 System Component Description
4.3.1 Standard Components
The standard system components are:
• Sonifier unit
• Power cord
• Converter
• Horn (and tips)
Con-
Ultrasonic Power
Supply Module
Converter
and
Horn
100-214-239 Rev. A 4-3
System Component Desc ription
Digital Sonifier
4.3.2 Optional Items
A complete list of optional items is provided in Appendix C of this Guide.
• Printer: Epson FX-compatible dot-matrix printer with a parallel-port interface
with a DB25M cable connector only.
• Remote Terminal or Comput er: Any user-provide d computer o r terminal that
allowsAS C II data to be transferred over an RS-232 serial interface. The pin-out
of the serial connec tor is listed in ’Remote Terminal Connection’ on page 4-14.
The computer interface, s oftware and setup are for the reader to determine.
• Temperat ure Probe: Omega Technologiesmodel #OL-703-PP, 0.125 inches in
diameter and 4.5 inches in length. This item is sold separately by Branson.
Refer to the
Appendix B, Optional Items Parts List
, for information about specific parts needed
for the optional items.
4.3.3 Accessories
Disruptor horns, various horn tips, and a variety of chambers or vessels for batch or continu-
ous processing are available for use with Branson Digital Sonifiers. For a list of Accessories,
refer to
Appendix C, Accessories Parts List
.
Microtips
Particularly useful for processing small volumes, microtips are available in several designs,
tapered and stepped, to meet your application requirements. A Coupling Section may be used
with a microtip horn for certain applications.
Microtips, Coupling Section and Disruptor Horn
Double Step
Tape red Microtip
Coupling
Section
Microtip
Disruptor horn
Note: Do Not
Use with
Disruptor Horn
4-4 100-214-239 Rev. A
Installation and Setup
CAUTION
j DO NOT allow the horn or microtip to contact lab stands, beakers, test tubes
or simil ar objects. Microtip failure may result. Breakage of glassware may
result in the loss of a specimen.
Disruptor horns are the base for many microtip applications. Disrupter horns are threaded so
that they may also be inserted into a Flow-Thru chamber attachment (see page 4-6). When
threaded together, a closed chamber is formed between the Tissue Disruptor and the cavity of
the Flow-Thru Attachment. The Tissue Disruptor may also be used alone.
The Tapered Microtip attaches directly to a standard 1/2 inch tapped Disruptor horn. The
amplitu de at the end of a tap ered tip is thr ee and one- hal f times g re ate r tha n tha t of the sta n-
dard horn. The tapered tip is recommended for difficult applications such as spores, fungi,
yeast, muscle, and connective tissue. Excellent results can be achieved on volumes ranging
from 3 to 20 ml in a comparatively short period of time. The diameters of the tapered micro
tips are 1/8 inch (3.2 mm), 3/16 inch (4.8 mm), and 1/4 inch (6.4 mm).
The stepped microtip is a two-piece unit, consis ti ng of a Coupling Section and a lower double-
step tip. Because the coupling section is attached directly to the converter, the standard disrup-
tor horn must be removed prior to using the stepped microtip. Recommended for use on
extremely small volumes, the stepped microtip can be used to treat volumes ranging from 0.5
to 20 ml. Applications for this tip include red and white blood cells, tissue culture cells, HeLa
cells, and the complete range of cells that have low to medium resistance to breakage.
To prevent foaming or aerosoling while processing small quantities with the tapered or stepped
microtips, the us e of a conical-sh ap ed tube s uch as a reaction vial or a cut down centrifuge tube
is recommended.
CAUTION
j When u sing microtips, do not exceed a maximum Amplitude Control setting
of 70%. The microtip will break if driven at higher amplitu des .
Tissue Disruptor
Designed for disintegration of difficult tissues, this stainless steel accessory has a specially con-
structed cell bottom that holds up to six grams of tissue. A water jacket may be provided for
cooling.
100-214-239 Rev. A 4-5
System Component Desc ription
Digital Sonifier
Cup Horn
Cup Horns allow materials to be treated in small vials or test tubes without immersing the
ultrasonic horn or micro tip in the material, thereby providing completely sterile conditions. A
Cup Horn is attached directly to the converter, and the assembly is mounted upside-down on
the lab stand with the Cup Horn at the top. With chilled water in the Cup Horn, test tubes are
suspended in the Cup with the contents of the tubes just below the water level. Ultrasonic
energy is then conducted from the surface of the horn, through the water and test tube walls,
to the contents of the tube.
Some energy loss may occur when applying ultrasonics in this indirect way, and processing can
take longer than if the ultrasonic horn were immersed directly in the solution.
There are two types of Cup Horn, a high intensity unit that will accommodate a single test tube,
and a larger unit for multiple test tubes. The high intensity Cup Horn has a concave bottom
that focuses the ultrasonic energy on the bottom of the tube. The larger unit, with a diameter
of two or three inches, allows the immersion of multiple test tubes. The larger Cup Horns have
clear plastic walls, which permit easy viewing of the activity in the tubes during processing.
Both types of Cup Horn are designed to allow chilled water to circulate through the cup to
prevent heating of the solution as a result of the ultrasonic activity.
CAUTION
j The bottom of the t es t tubes should not be in contact with the surface of the
ultrasonic horn. Such contact c ould caus e break age and loss of samp le.
Cup Horn
Inlet / Outlet
Cavity
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