Bio-Rad Profinia Protein Purification System Buffers and Reagents User Manual

Profinia™Denaturing

IMAC Urea Pack

45 g, 90 g

Catalog Numbers

620-0200, 620-0201

Specifications

Quantity 45 g, 90 g
Description Profinia urea packs contain

high-purity chromatography
grade urea which is ideal for
solubilizing and denaturing
proteins in E. coli extracts.

Storage Store between 4°C and 22°C
Instructions Add the entire contents of the

for use 90 g pack to the 176 ml of

Profinia Denaturing IMAC
Lysis Buffer (#620-0209) or
Profinia Denaturing IMAC
W ash Buffer 1 (#620-0210) to
make 250 ml of a 6 M urea
solution. Add the entire contents
of the 45 g pack to the 88 ml of
Profinia Denaturing IMAC
W ash Buffer 2 (#620-0211) or
Profinia Denaturing IMAC

2