Bio-Rad Prestained SDS-PAGE Standards User Manual

Reference

1. Laemmli, U. K., Nature, 227, 680 (1970).

Ordering Information

Catalog
Number Product Description

Prestained Standards

161-0305 Prestained SDS-PAGE Standards, low range, 500µl
161-0309 Prestained SDS-PAGE Standards, high range, 500 µl
161-0318 Prestained SDS-PAGE Standards, broad range, 500 µl
161-0324 Kaleidoscope Prestained Standards, 500 µl
161-0325 Kaleidoscope Polypeptide Standards, 500 µl

Molecular Weight Standards

161-0304 SDS-PAGE Standards, low range, 200 µl
161-0303 SDS-PAGE Standards, high range, 200 µl
161-0317 SDS-PAGE Standards, broad range, 200 µl
161-0314 Silver Stain SDS-PAGE Standards, low range, 200 µl
161-0315 Silver Stain SDS-PAGE Standards, high range, 200 µl
161-0306 Biotinylated SDS-PAGE Standards, low range, 250 µl
161-0311 Biotinylated SDS-PAGE Standards, high range, 250 µl
161-0319 Biotinylated SDS-PAGE Standards, broad range, 250 µl
161-0326 Polypeptide SDS-PAGE Standards, 200 µl

Specialty Standards

161-0310 IEF Standards, pI range 4.45-9.6, 250 µl
161-0320 2-D SDS-PAGE Standards, 500 µl

Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547

4006024 Rev D

Prestained

SDS-PAGE Standards,

Low Range

Catalog Number

161-0305

Product shipped at room temperature.

Store at -20 °C upon arrival

Bio-Rad’s prestained standards are available
in high, low, and broad molecular weight
ranges. Blue dye has been covalently attached to
the standard proteins and will not be dissociated
by normal staining or destaining. The protein
mixtures are provided in a stable aqueous solu­tion. No reconstitution or further dilution is
required before use.

Applications

Prestained SDS-PAGE Standards provide a
quick and easy way to assess the quality of an
electrophoretic transfer and act as a control in
repetitive blotting experiments. The visibility of
the standards makes it possible to monitor the
separation of proteins while electrophoresis is
in progress, even after the dye front has run off
the gel. The standards can also be used to locate
a protein for excision from an unstained prepar­ative gel.

Specifications

Contents Approximately 625 µg total protein*

in 33% (v/v) glycerol, 3% SDS, 10
mM Tris pH 7.0, 10 mM DTT, 2 mM
EDTA, 0.01% NaN3.

Storage -20 °C
Shelf life 1 year at -20 °C
Volume 500 µl
Applications 25-100

per vial

* Total protein concentration is an approximation based

on the average concentrations of multiple lots. Actual
concentrations will vary.

Coomassie is a trademark of ICI.

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