Bio-Rad Prepacked Gravity Flow Columns for Ion Exchange User Manual
Prefilled Poly-Prep
Columns for
Ion Exchange
Chromatography
Instruction Manual
®
Table of Contents
Section 1 Introduction ............................1
Section 2 Column Description................2
Section 3 Instructions For Use...............2
Section 4 Ion Exchange Capacity ..........3
Section 5 Flow Rate Characteristics......4
Section 6 Chemical Compatibility.........5
Section 7 Temperature Limits ...............6
Section 8 Troubleshooting......................6
Section 9 Ordering Information ............8
Section 1
Introduction
Prefilled Poly-Prep columns are small
polypropylene columns containing ion
exchange resins. These columns combine the
analytical quality and wide selection of
Bio-Rad’s ion exchange resins with the convenience of small, disposable columns. Poly-Prep
columns are especially useful in sample preparation for HPLC, GC, IC, and AA analysis.
Multi-purpose cap
Volume markings
10 ml reservoir
2 ml bed
(fully visible)
Fig. 1. Poly-Prep column.
1
Built-in bed support
Luer tip fitting
Snap-off seal
Poly-Prep columns are ideal for routine
applications in which a small amount of chromatographic medium is used and then discarded, or for multiple sample clean-up applications. They are a time-saving and versatile
replacement for Pasteur pipets and other
improvised columns.
Section 2
Column Description
The graduate 0.8 x 4 cm columns, constructed of high density polypropylene, hold a
standard bed volume of 2 ml of chromatographic media, and include an integral 10 ml
reservoir. A Kynar
umn acts as a bed support for the resin packing. At the bottom of the column is a snap-off
seal, which leaves a male Luer tip fitting when
it is removed.
Prefilled Poly-Prep columns are shipped
sealed, to prevent the resin packing from drying. Columns are packed in deionized water.
During transport, the resin may move to the
top of the column. This is corrected by shaking
the column and allowing the resin to settle
before use. The columns should be stored
upright, in a dark, cool location.
®
20 µm frit inside the col-
Section 3
Instructions for Use
1. Shake the column and allow all the resin to
settle to the bottom.
2
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