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Catalog # Description
161-0 363 Precision Plus Protein Unstained Standards, 1,000 µl
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161-0747 4x Laemmli Sample Buffer, 10 ml
161-0786 Bio-Safe
Cooma ssie is a trademark of BAS F Akti enges ellsc haft.
Tween is a trade mark of I CI Americas I nc.
This product is sold under license from Life Technologies Corporation,
Carls bad, CA, fo r use onl y by the buyer of the pro duct. T he buyer is not
authorized to sell or resell this product or its components.
For more information, go to www.bio-rad.com.
4110182 Rev G 13-0322 0313
Pack, 5 x 1000 µl
Pack, 5 x 500 µl
Pack, 5 x 500 µl
Pack, 5 x 500 µl
Plus Prote in Wester nC Sta ndards a nd Stre pTactin-HRP,
50 applications
250 applications
™
Coomassie Stain, 1 L
™
WesternC™ Pack, Precision
Electrophoresis
Precision Plus Protein
Kaleidoscope™ Standards
Instruction Manual
Single unit
Catalog #161-0375
5-Pack value pack
Catalog #161-0395
Product shipped at
room temperature.
Store at –20°C for 12 months.
™
Representative lot
of Precision Plus
Protein Kaleidoscope
prestained standards
on a Criterion
4–20% Tris-HCl G el
™
250
150
100
The Precision Plus Protein standards are
kD
the latest generation of recombinant proteins
designed to provide a ladder of convenient,
consistent sizes. These standards continue the
tradition of accuracy and ease of use begun
75
50
37
25
20
15
10
by Precision Protein
providing instant orientation, even greater
accuracy, particularly in the 10–25 kD range.
The Precision Plus Protein Kaleidoscope
standards provide a broad range recombinant
ladder with multiple colors that allow for easy
band referencing and blot orientation.
Note: Prestained standards may fade during blocking,
antibody incubation, or washing steps due to the use of
Tween-20 in buffers. For other options, contact Bio-Rad
Technical Suppor t at 1-800-424-6723.
™
standards, while also
Product Description
Precision Plus Protein Kaleidoscope standards
contain ten protein bands of 10, 15, 20, 25, 37,
50, 75, 100, 150, and 250 kD.
Recombinant prestained protein standards are
carefully engineered for precise and accurate
molecular weights. The staining process has been
optimized to guarantee the same electrophoretic
molecular weight with each lot. Traditional
prestained proteins, on the other hand, are
blended from naturally occurring proteins. They
have an inherent variability in the amount and
location of dye that covalently binds to the protein;
this produces diffuse, broader bands than those
of the recombinant prestained standards and the
resulting molecular weights vary from lot to lot.
Molecular Weight Estimation
The molecular weights of Precision Plus Protein
Kaleidoscope standards are confirmed by
migration in a Laemmli SDS-PAGE system.
Using Precision Plus Protein Kaleidoscope
standards, the molecular weight of an unknown
protein can be assessed at an accuracy of 95%.
For electrophoretic determination of molecular
weights at an accuracy of >95%, Precision Plus
Protein Unstained standards (catalog #161-0363)
should be used.
These standards are also compatible with the
XT buffer system, but should only be used for
molecular weight estimation. For molecular weight
determination on XT gels, use the Precision Plus
Protein Unstained or All Blue standards.
Instructions for Use
Precision Plus Protein Kaleidoscope standards
are provided in loading buffer, ready to load,
with no dilution required. Allow the tube to reach
room temperature and thoroughly mix before
use. This will ensure that any solids that may
have precipitated have returned to solution. Do
not heat the product above room temperature.
Recommended Loading Volumes
Load Volume Application
5 μl Blotting mini (8.3 W x 6.4 L cm) and midi
10 μl Mini and midi gel electrophoresis
20 μl Large (18.3 W x 19.3 L cm) gel electrophoresis
Specifications
Catalog # 161-0375, single unit
Volume 500 μl
Number of 50, single unit
applications 250, 5-pack value pack
Loading 30% (w/v) glycerol, 2% SDS, 62.5 mM Tris,
buf fer pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN
0.01% bromophenol blue
Shelf life
Total protein 1.5 mg/ml
concentration
(13.3 W x 8.7 L cm) gels, monitoring transfer
161-0395, 5-pack value pack
12 months at –20°C; if the product has been
stored as directed for longe r than 12 months,
the shelf life may be extended
dithiothreitol (DTT) to 50 mM
by adding
,
3
3
2
1
Log MW
Fig. 1. r
standards determined on a Criter ion 4 –20% gel.
>0.99 are typical for Precision Plus Protein Kaleidoscope standards.
r2 = 0.996
0
0 0.5 1.0
2
plot for Precision Plus Protein Kaleidoscope
Relative front
2
r
values
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