Binder CB User manual
CB (E2) Service Manual
state: 01/2002 created: 03/2002 / Jochen Tussinger
CB (E2) Service Manual
08:43:55 20.11.01
W
X
4.9
25.2
36.8
EXIT
°C
%
%
TEMP
TEMP
37.0
C2O
5.0
O2
25.0
CONFIG W MENUE VIEW->
RESET
Version of the described Chamber :
Standard equipped CB CO2 Incubator E2
with
FPI Sensor System
and
MB1 Controller
SERIAL-NO. 01-27954 à
Order-No. 9040-0012 / CB 150
Order-No. 9040-0013 / CB 210
CB (E2) Service Manual
state: 01/2002 created: 03/2002/ Jochen Tussinger
Contents
1 Modification levels...................................................................................................................3
2 Unit overview..........................................................................................................................4
2.1 The Controller MB1...........................................................................................................5
2.2 Short description of the MB1 Controller..............................................................................6
3 Function ...............................................................................................................................12
3.1 The CO2-measuring principle........................................................................................... 12
3.2 Function of the Heating System....................................................................................... 12
3.3 Flow-Chart of the heating function (basis CB 150 wiring diagram) ......................................13
3.4 Controller MB1 PIN description (Input / Output)................................................................ 14
3.5 Function of the CO2 System ............................................................................................15
3.6 Flow Chart of the CO2 System (basis CB wiring diagram) ..................................................15
3.7 Function of the Permadry® system.................................................................................. 16
3.8 Flow Chart of the Permadry® system (basis CB wiring diagram)........................................ 17
3.9 Description of the Function of the Fan Control ..................................................................17
3.10 Sterilization Mode ........................................................................................................18
3.11 Hot-air sterilization .......................................................................................................18
3.12 Hot-air sterilization with inner chamber contaminated with highly infective material ..........20
4 Trouble Shooting................................................................................................................... 22
5 Most common service work.................................................................................................... 25
5.1 Changeing of the fan....................................................................................................... 26
5.2 Take out of the electronic component board .....................................................................28
5.3 Opening of the rear service lid to achieve the area of the air jacket ....................................29
5.4 Setting of the door heating ...............................................................................................31
5.5 CO2-Reference Measurement.......................................................................................... 32
5.5.1 Measuring of CO2 indirectly via the pH of the cell medium ..........................................33
5.5.2 Measuring of CO2 directly via chemical indicator tubes............................................... 34
5.5.3 Measuring of CO2 directly via a electronic measuring device....................................... 35
6 Calibration............................................................................................................................ 36
6.1 Definition of calibration.................................................................................................... 36
6.2 References for calibration ................................................................................................36
6.3 Tolerance of the adjustment ............................................................................................36
Calibration instructions for CO2 incubator CB with screen controller MB1........................................37
Temperature / CO2 / O2 controller ................................................................................................37
Temperature calibration ............................................................................................................37
Calibration (alignment) of the temperatur e controller ...................................................................38
Reading out of the actual values:............................................................................................... 39
Entries: ....................................................................................................................................39
Result Calibration (alignment) of the temperature controller......................................................... 39
CO2 calibration (alignment)........................................................................................................ 40
O2 calibration (alignment).......................................................................................................... 41
7 Maintenance......................................................................................................................... 48
8 Explosion Drawings of the CB /E2 ..........................................................................................51
8.1 CB Component Board .....................................................................................................51
8.2 CB Kettle / Sensors / Heaters / Permadry......................................................................... 52
8.3 CB Door / Sealing / Inner parts ........................................................................................53
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CB (E2) Service Manual
state: 01/2002 created: 03/2002/ Jochen Tussinger
1 Modification levels
The CB CO2 Incubator E2 is a further development of CB CO2 Incubator E1. Especially the Display
controller MB1 inside the red triangle is conspicuous.
The whole electronic is placed inside the lower part of the CB CO2 Incubator (Front Access
Maintenance). Theres no I-box as at the CB CO2 Incubator E1 at the top of the chamber.
08:43:55 20.11.01
W X
TEMP
TEMP
37.0
5.0
25.0
36.8
4.9
25.2
RESET
C2O
O2
CONFIG W MENUE VIEW->
EXIT
°C
%
%
Display Controller MB1
Display controller MB1
Machine Room with all electronic parts
3
CB (E2) Service Manual
Machine room with all
(M)
(F) (K)
state: 01/2002 created: 03/2002/ Jochen Tussinger
2 Unit overview
(B)
(C)
(D)
(E)
(G)
(H)
(I)
(J)
(A)
FRONT
electronic parts
(N)
(L)
A) Display controller MB1 for temperature and CO2 as well as O2 (option)
B) Connection socket for low tension supply (option)
C) CO2 sensor
D) Gas mixing head
E) PT 100 temperature probe
F) O2 sensor (option)
G) Internal socket 230V (max. 3 A) (option)
H) Shelf holder bar
I) Shelves
J) Shelf holder
K) Lower housing cover
L) Permadry® water basins
M) Inner glass door
N) Measuring access port
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CB (E2) Service Manual
5.0
VIEW->
Display Controller MB1
state: 01/2002 created: 03/2002/ Jochen Tussinger
2.1 The Controller MB1
Operating Light
08:43:55 20.11.01
W X
TEMP
37.0
TEMP
C2O
O2
25.0
36.8
4.9
25.2
EXIT
°C
%
%
EXIT-Button
(to leave a
Menue)
CONFIG W MENUE VIEW->
RESET
ON / OFF Button
(Stand-by)
ENTER-Button
((to confirm a
selection)
Navigation buttons
(corresponding function
will be assigned)
Mainswitch
CONFIG
W
RESET
For the controller MB1, it is possible to show all setpoints an d actual values at the same time.
It is also possible to display the actual values portrary.
MENUE
5
CB (E2) Service Manual
state: 01/2002 created: 03/2002/ Jochen Tussinger
2.2 Short description of the MB1 Controller
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,,
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CB (E2) Service Manual
state: 01/2002 created: 03/2002/ Jochen Tussinger
3 Function
Incubators series CB are suited for cultivation of mammal cells under typical conditions of about 37°C.
The incubator allows the set defined pH conditions by justifying NaHCO3 buffer systems of commercial
cell media by keeping exact CO2-atmosphere inside. CB incubators care for high humidity inside to
avoid increasing of the osmolarity caused by evaporation of the cell media. To reach this, the different
functions heating, sterilization and CO2 injection have to work as a perfect team. This know-how is as
Firmware inside the controller type MB1.
3.1 The CO2-measuring principle
The CO2-measuring procedure of the incubator series CB is characterized by fast reaction times, as
well as the highest accuracy and selectivity. The accuracy of the CO2 measuring system bases on a
single-beam infrared measuring cell, which measures in di fferential mode, with permanently
alternating transmission characteristic of its semi-conductor filter. Due to this highly developed single beam principle with Fabry -Perot interferometer (FPI), disturbance variables and aging phenomena in
the measuring system are almost completely eliminated, so that this measuring system, in contrast to
other measuring procedures, remains practically drift -free between calibrations and is absolutely
selective for CO2.
The CO2–measuring cell contains a measuring section ins ide in which the absorption of infrared light
depends on the number of CO2-molecules in the beam path. This number of CO2 –molecules changes
with the ambient pressure in relation to a constant volume. The distances between the molecules are
consequently pr essure-dependent. The collision frequency of the IR-beam with CO2-molecules
increases therefore by increasing pressure.
For this reason, the ambient pressure must be compensated in order to correct the display reading of
the CO2-concentration in VOL. -%. This is achieved by entering the altitude of the site above the sea
which is described in this manual.
3.2 Function of the Heating System
The temperature measurement is realized by a PT100 temperature probe which changes his resistance
at different temperatures . For example: 37°C = 114,380 Ω (see following chart).
The CB is equipped with a double PT100, one part is for the measurement inside the chamber, the
other part is connected to the safety device class 3.1.
The measured value is evaluated in the controller MB1. The controller MB1 compares the set -value
and the now measured value and decides to give a signal-current to the solid state relay –4K3 to
activate the heating.
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CB (E2) Service Manual
Measuring of the Pt100 resistance between the white
state: 01/2002 created: 03/2002/ Jochen Tussinger
3.3 Flow-Chart of the heating function (basis CB 150 wiring diagram)
The Pt100 temperature probe is equipped with 3 cables, two red and one white cable.
The white cable is connected to pin 1 at E1 – Pt100, the two red cables at pin’s 2 and 3 at E1 – Pt100.
To measure the resistance disconnect all three cables from the controller an measure between the
white cable and one of the red cables, do not measure between both red cables.
Ω
cable PIN 1 and one of the two red cables PIN 2 or 3
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CB (E2) Service Manual
BE1 BE2 BE3 BE4
Output Phase
-
controll
3A / 115
-
230V AC
Heater air
-
duct
BA2 – 1A / 115
-
230V AC
Door Heater
BA3 – 1A / 115
-
230V AC
Collection Alarm
BA1 – 3A / 115
-
230V AC
Ground TE
Heater Kettle
BA4 – 60mA / 5V DC
CO2 Solenoid valve
BA5 – 60mA / 5V DC
Safety device Cl 3.1
BA6 – 60mA / 5V DC
Fan Cold water bassin
0(2)-10V / 0(4)
-
20mA
Volatge output
45mA / 24VDC
state: 01/2002 created: 03/2002/ Jochen Tussinger
Pt100 temperature probe (Temperature in °C / Resistance in Ω)
T (°C) 0 1 2 3 4 5 6 7 8 9 10
-10
0
10
20
30
40
50
60
70
80
90
100
96,086 96,478 96,870 97,262 97,653 98,045 98,436 98,827 99,218 99,609 100,000
100,000 100,391 100,781 101,172 101,562 101,953 102,343 102,733 103,123 103,513 103,902
103,902 104,292 104,681 105,071 105,460 105,849 106,238 106,627 107,016 107,404 107,793
107,793 108,181 108,570 108,958 109,346 109,734 110,122 110,509 110,897 111,284 111,672
111,672 112,059 112,446 112,833 113,220 113,607 113,994 114,380 114,767 115,153 115,539
115,539 115,925 116,311 116,697 117,083 117,469 117,854 118,240 118,625 119,010 119,395
119,395 119,780 120,165 120,550 120,934 121,319 121,703 122,087 122,471 122,855 123,239
123,239 123,623 124,007 124,390 124,774 125,157 125,540 125,923 126,306 126,689 127,072
127,072 127,454 127,837 128,219 128,602 128,984 129,366 129,748 130,130 130,511 130,893
130,893 131,274 131,656 132,037 132,418 132,799 133,180 133,561 133,941 134,322 134,702
134,702 135,083 135,463 135,843 136,223 136,603 136,982 137,362 137,741 138,121 138,500
138,500 138,879 139,258 139,637 140,016 140,395 140,773 141,152 141,530 141,908 142,286
For Example: Your resistance measurement system shows you 114,380 Ω this corresponds to 37°C.
3.4 Controller MB1 PIN description (Input / Output)
E1 – Pt100
E2 – Pt100
E3 – Pt100
E4 – 4 - 20mA
E2 – Pt100
E – Safety device class 3.1
Display connection
RS422
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CB (E2) Service Manual
To A1+ / A2
-
1 – white
state: 01/2002 created: 03/2002/ Jochen Tussinger
3.5 Function of the CO2 System
The CO2 System measures the CO2 concentration inside the chamber. This information is supplied by
the FPI Sensor Head. The Controller MB1 compares the engaged value with the now measured value
and decides to open the solenoid valve of the CO2 gas inlet.
3.6 Flow Chart of the CO2 System (basis CB wiring diagram)
FPI-Sensor à FPI Sensor Board à Controller MB1 à Solenoid Valve à Gas Injection Nozzle
2 – grey
3 – green
4 – yellow
5 – blue
6 – red
7 – brown
8 - pink
To Power supply at the Transformer
H1+
Solenoid Valve CO
2
1
-5Y1
2
H2-
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CB (E2) Service Manual
Lettering
state: 01/2002 created: 03/2002/ Jochen Tussinger
3.7 Function of the Permadry® system
Isotonic osmotic pressure ratios, essential for the growth of cells, are basically maintained in CO2
incubators by a maximum humidity content in the inner chamber. During this process care must be
taken to ensure the best possible protection against contamination.
The patented Permadry® system guarantees a humidity performance of up to 98% relative humidity
with completely dry inner walls. The principle hereof is totally easy. The double basin system consists
of a large-surface warm water basin an a cold water basin as defined condensation point. The
temperatures of both basins are in that way controlled that the humidification and the dehumidification
are permanently balanced. The Permadry® system works completely free of disturbances or
maintenance. The handling of the Permadry® system is the easiest thing and as safe as a coffee cup.
The basin is easily removable and can be refilled in or at the unit at any time.
Cold air, produced by the Permadry® -fan, streams through the air -channel and cools the round cold
water basin. The condensation point is fixed by this.
The Permadry® water basin must be placed correctly. There is a description „FRONT“ impressed.
„FRONT“
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