Agilent Technologies PCR Polishing INSTRUCTION MANUAL

PCR Polishing Kit
INSTRUCTION MANUAL
Catalog #200409
Revision A
For In Vitro Use Only
200409-12
LIMITED PRODUCT WARRANTY
ORDERING INFORMATION AND TECHNICAL SERVICES
United States and Canada
Agilent Technologies Stratagene Products Division
11011 North Torrey Pines Road La Jolla, CA 92037
Telephone (858) 373-6300 Order Toll Free (800) 424-5444 Technical Services Internet World Wide Web
techservices@agilent.com
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All Other Countries
Please contact your local distributor. A complete list of distributors is available at www.stratagene.com.
PCR Polishing Kit
CONTENTS
Materials Provided.............................................................................................................................. 1
Storage Conditions.............................................................................................................................. 1
Additional Materials Required .......................................................................................................... 1
Introduction......................................................................................................................................... 2
Precautionary Notes............................................................................................................................ 2
Protocol ................................................................................................................................................ 3
PCR Polishing Reaction ........................................................................................................ 3
Standard Blunt-End Ligation of Control DNA...................................................................... 5
Preparation of Media and Reagents.................................................................................................. 6
References ............................................................................................................................................ 6
Endnotes............................................................................................................................................... 6
MSDS Information.............................................................................................................................. 6
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