3M DELI User Manual
Zeta Plus™
DELI and DELP
Zeta Plus™ DELI and DELP series comprise a family of depth filter media which incorporates lipid-specific adsorbents for sel ective reduction of lipids, surfactants and biological
hydrophobes from human blood plasma, serum-based products, recombinant proteins and
other biological fluids. These media types provide a range of lipid reduction capacity and
functionality.
Applications
During protein purification processes, lipid contamination can cause an assortment of processing problems. Lipids can foul and plug expensive chromatography columns and membrane filters. Prior reduction of lipids via depth filtration promotes column and membrane
performance and extends their useful life. Lipid contamination can also cause solution
instability of intermediate and final protein products leading to a problematic lipoprotein
haze. Zeta Plus DELI and DELP series products can replace bulk silica operations which
are labor-intensive, messy and difficult to prepare.
Series Filter Media
Zeta Plus DELI and DELP series filter media may be used to separate lipids within the
following lipid classes: mono-, di-, and triglycerides, fatty acids, cholesterol, phospholipids, and lipoproteins. Enhanced reduction of lipid contaminants from protein solutions is
therefore highly desirable, leading to improved processing economies (extended column
and membrane life), improved product purity, and enhanced final protein product stability
for longer product shelf life.
Features & Benefi ts
Two different media formulations available for varying degrees of lipid reduction.
■
Effi cient reduction of undesirable lipids, lipoprotein haze, and microbial and
particulate contaminants.
Depth fi lter media with hydrophobe and lipid specifi c adsorbents.
■
Provides effective protection and extended life for chromatography columns and
reusable membranes.
Variety of cartridge sizes, disposable capsules and fi lter sheets.
■
Highly scaleable.
■
Flexible options for all fl ow and batch volume requirements.
Totally-enclosed, sanitary-design systems and cartridge housings.
■
Easily cleanable, no external contamination, offers rapid assembly and take down.
Tested and optimized for pharmaceutical and biological service.
■
Safe for critical pharmaceutical, bioprocessing and biological applications.
■
Components meet USP Class VI Biological Safety Tests and requirements of CFR 21.
Applications
Lipid reduction from human blood plasma
proteins such as albumin, coagulation factors,
fi brinogen, and immunoglobulins
Provide protection, enhanced performance and
extended life for chromatographic columns and
ultrafi lters
Reduction of residual lipids from serum, fermentation broths and cell culture fl uids
Reduction of detergents and surfactants from
biologicals
Separation of hydrophobic bioprocess contaminants
from aqueous solutions
Filter
)
DELI
DELP
M
17.9
0
9
0
5
0
Media
Protected
Flow Channels
Gasket
Retainer
Internal
Separator
Cell
Weldments
Various
Gasket Materials
Edge
Edge
Seal
Tab s
Cell
Separator
Zeta Plus ™ Products: The Benchmark for Pharmaceutical & Biological Depth
Filtration
Filters using Zeta Plus™ DELI and DELP series media are part of the Zeta Plus family
of depth filtration pr oducts, which provide highly efficient, cost-effective clarification
of turbid, difficult-to-filter biological fluids and pharmaceutical products. Zeta Plus
cellulose-based depth filtration media are designed to retain contaminants by both size
exclusion and adsorption. It is composed of high surface area filter aids embedded in a
cellulose fiber matrix.
Zeta Plus DELI and DELP filter media incorporate added silica adsorbents, which are the
active components for lipid reduction. During the manufacturing process, mol ecules
carrying a positive charge are chemically bonded to the matrix components, forming a
permanent, interconnected, rigid filter sheet with positive ly -charged electrokinetic capture
sites. The resulting porous depth filter structure is a tortuous network of adsorptive flow
channels capable of retaining bacteria, endotoxin, cell debris and su bmicron con taminants
to a level which mechanical screening alone cannot achieve. Zeta Plus depth filters provide
high-efficiency submicron filtration, often surpassing the performance of pleated prefilters
and competitive depth filters.
Zeta Plus Delipid Media Choices
T w o different types of delipid filter media are available to suit varying application requirements. The chart below serves as a guideline for selecting the appropriate delipid media
type:
Type Lipid Reduction Capacity
DELI Intermediate No No
DELP High Yes Yes
*
Limulus
Amoebocyte Lysate
Optimized for Low Aluminum
Extractable Levels
Optimized for Sensitive
LAL* Test Procedures
All DELI and DELP fi lter media are available in totallydisposable BC fi lter capsules for lab-scale, process
development or small-volume fi ltration. Shown are
BC25 and BC1000/2000 capsule units with DELI
fi l t e r m e d i a .
2
Media Selection
3M differentiates its DELI and DELP media offerings by varying the materials of construction used in their manufacture. Choice of silica type confers upon the media either a
medium or a high capacity for lipids based upon the specific surface area (m²/g) of the silica
incorporated into the filter matrix. Choice of cellulose type affects the level o f apparent
endoto xin or Limulus Amoebocyte Lysate-R eacti v e Material (LAL-RM) extractables .
LAL Reactive Material
Trace levels of cellulosi c impurities (ß-glucans ) from several of the more useful types of
cellulose interfere with some (but not all) FDA-approv ed LAL reagent systems designed
to test for USP Bacterial Endotoxins. DELP media has been purposely formulated to alleviate this sort of interference.
Table 1: LAL Reactive Material (EU/ml
Rinse Volume l/m
EU/ml including LAL-R
35.
3.8
0.50
.25 0.01
.25 0.01
.02
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