YSI ProOBOD User Manual

USER MANUAL

CONTENTS

Warranty ..............................................................................................................i

Introduction ....................................................................................................... 1

Getting Started ................................................................................................... 2

Unpacking the OBOD Probe ............................................................. 2

POBOD Operation ........................................................................................4

Connecting Probe/Cable Assembly to Instrument .........................4

Configuring the PODO Instrument .............................................4

Powering the Stirrer ............................................................................5

Calibration - Dissolved Oxygen ........................................................ 5

Temperature......................................................................................... 7

Taking Measurements ......................................................................... 7

Maintenance and Storage .................................................................................. 8

Updating Instrument and Probe Firmware ...................................... 8

Sensor Maintenance - Dissolved Oxygen ......................................... 8

Sensor Maintenance - Temperature ................................................ 11

Sensor Storage ................................................................................... 12

Item #626471
Rev B
Drawing # A626471
September 2010

©2010 YSI Incorporated.

The YSI, ProODO, OBOD and ODO logos are
registered trademarks of YSI Incorporated.

Troubleshooting ...............................................................................................12

Dissolved Oxygen Readings .............................................................12

Help ...................................................................................................13

Error/Status messages ...................................................................... 13

Restore Default Calibration Values ................................................14

Probe Specifications .........................................................................................15

Accessories / Parts List .................................................................................... 16

Declaration of Conformity ..............................................................................17

Recycling ...........................................................................................................19

Contact Information ........................................................................................ 20

Ordering and Technical Support .....................................................20

Service Information .......................................................................... 20

Appendix A-DO% Calibration Values ........................................................... 21

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WAR R A N T Y

The YSI Professional OBOD® (ProOBODTM) probe and cable assembly is warranted
for two (2) years from date of purchase by the end user against defects in materials and
workmanship, exclusive of batteries and any damage caused by defective batteries.
Pro OBOD se nsor ca ps are wa rr anted f or on e (1) yea r f rom dat e of pu rc hase b y t he en d
user against defects in material and workmanship. ProOBOD systems (instrument
& cable/probe assemblies) are warranted for 90 days from date of purchase by the
end user against defects in material and workmanship when purchased by rental
agencies for rental pu rposes. Within the warranty period, YSI wi ll repair or replace,
at its sole discretion, free of charge, any product that YSI determines to be covered
by this warranty.

To exercise this warranty, call your local YSI representative, or contact YSI
Customer Serv ice in Yellow Springs, Ohio at [phone], 800-897-4151 or v isit
www.YSI.com for a Product Return Form. Send the product and proof of purchase,
transportation prepaid, to the Authorized Service Center selected by YSI. Repair
or replacement will be made and the product returned, transportation prepaid.
Repaired or replaced products are warranted for the balance of the original warranty
period, or at least 90 days from date of repair or replacement.

LIMITATION OF WARRANTY

This Warranty does not apply to any YSI product damage or failure caused by:

Fai lure to install, operate or use the product in accordance with YSI’s written 1.
instructions;
Abuse or misuse of the product; 2.
Failure to maintain the product in accordance with YSI’s written instructions 3.
or standard industry procedure;
Any improper repairs to the product; 4.
Use by you of defective or improper components or parts in servicing or 5.
repairing the product;
Modification of the product in any way not expressly authorized by YSI.6.

THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR
IMPLIED, INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS
FOR A PARTICULAR PURPOSE. YSI’S LIABILITY UNDER THIS WARRANTY
IS LIMITED TO REPAIR OR REPLACEMENT OF THE PRODUCT, AND THIS
SHALL BE YOU R SOLE AND EXCLUSIVE REMEDY FOR ANY DEFECTIVE
PRODUCT COVERED BY THIS WARR ANTY. IN NO EVENT SHALL YSI BE
LIABLE FOR ANY SPECIAL, INDIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES RESULTING FROM ANY DEFECTIVE PRODUCT COVERED BY
THIS WARRANTY.

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1

INTRODUCTION

Thank you for purchasing the YSI Professional Optical Biochemical Oxygen
Demand (ProOBOD) probe. The ProOBOD is designed for use with a YSI
ProODO® instrument. The probe will not work with any other YSI instrument.
This manual provides information on the ProOBOD probe only. For more
detailed instructions on how to operate the ProODO instrument, please refer to
the ProODO User Manual (www.ysi.com/ProODO).

The ProOBOD is designed to fit in a standard 300 ml BOD bottle as well as other
international BOD bottles, depending on the version of the probe. YSI offers
two probe versions:

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ProOBOD USA (Japan), YSI item number 626400 - This probe fits in •
standard USA and Japanese style BOD bottles. A power supply for
USA and Japanese style AC outlets is included.

ProOBOD International, YSI item number 626401 - This probe fits •
in standard BOD bottles used outside the USA and Japan. A funnel
adapter for fitting in standard UK BOD bottles and a switching power
supply with 3 international outlet adapters are also included.

The ProOBOD sensor measures dissolved oxygen in water using lifetime
luminescence technology and uses a digital signal to send information between
the instrument and probe. The probe features a stirrer and motor to aid in
sample movement, to keep solids from settling at the bottom of the sample bottle,
and to speed up the response of the sensor. Key advantages of the ProOBOD’s
use of optical technology for DO measurement over traditional electrochemical
methods include the elimination of sensor flow dependence and sensor warm­up time, greater stability, the ability to zero the sensor for more accurate
measurements at low dissolved oxygen levels, and the elimination of frequent
membrane/electrolyte changes and electrode servicing.

For more information on the ProODO instrument and product specification,
please visit www.ysi.com/ProODO or contact Technical Support at 800-897­4151 ([phone]).

Reading the entire manual before use is recommended for an overall
understanding of the probe’s features.

Important: The ProOBOD is designed for laboratory use, not

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field use. It is not waterproof and should never be immersed
in a sample past the taper on the stem of the probe.

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3

GETTING STARTED

Throughout the manual, the term “probe” refers to the end of the cable where
the sensor, stir motor, and stirrer are located. The term “sensor” refers to the
Optical Dissolved Oxygen sensing portion of the probe assembly and the term
“sensor cap” refers to the removable sensing cap that is replaced about once per
year (Figure 1).

Stirrer toggle switch,

turns stir paddle

on and off

Probe

Temperature sensor

Optical DO sensor

Stir paddle

OBOD Sensor Cap,

shown here removed

from probe

Figure 1.

Remove the ProOBOD cable/probe assembly from the shipping container and
locate the instruction sheet that is included. This instruction sheet is important
because it contains the calibration coefficients for your sensor cap. After using
this sheet for general probe setup, be sure to store it in a safe place in case you
need to reload these calibration coefficients in the unlikely event that they are
ever deleted from the instrument and probe.

Note - A new cable/probe assembly already has a

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Preparing the probe for the first time:

A new ProOBOD probe will have a clear protective cover placed over the 1.
sensor cap to ensure the cap remains moist during shipment. Carefully
remove this clear cover from the end of the probe by pulling it straight off
the sensor. Save the clear cover along with the sponge in case you want to
use it later for long term storage of the probe.
Place the probe in a standard BOD bottle that contains a small amount of 2.
clean water (approximately 40 ml). The sensor should not be immersed in
water but rather in the air above the water. The purpose is to create a 100%
water-saturated air environment for the sensor which is ideal for calibration
and storage when not in use.

sensor cap installed and the sensor cap coefficients
are preloaded into the probe at the factory.

It is important to always keep your sensor in a moist
environment so the sensor cap does not dry out. (See

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Maintenance and Storage for more information.)

Unpacking the OBOD prOBe

Each ProOBOD probe and replacement OBOD sensor cap

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Carefully unpack the instrument and accessories and inspect for damage.
Compare received parts with items on the packing list. If any parts or materials
are damaged or missing, contact YSI Customer Service at 800-897-4151 (+1 937
767-7241) or the authorized YSI distributor from whom the instrument was
purchased.

includes an instruction sheet with important information
unique and specific to each individual sensing cap.