YSI Pro20 Operating Manual

PRO20

USER MANUAL

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Item # 605597

Drawing # A605597

The YSI logo is a registered trademark of YSI Incorporated.

Teflon is a registered trademark of E. I. du Pont de Nemours and Company.

Rev B

October 2008

CONTENTS

Warranty .....................................................................................................1

Introduction................................................................................................2

Getting Started ............................................................................................2

Initial Inspection................................................................................2

Battery Installation.............................................................................2

Keypad................................................................................................3

Connecting the Sensor and Cable.....................................................4

Membrane Installation ......................................................................7

Backlight.............................................................................................7

Powering Off ......................................................................................7

Run Screen .........................................................................................7

Navigation..........................................................................................8

First Power On ...................................................................................9

System Setup Menu ..................................................................................10

DO Local%.......................................................................................10

Last Digit Suppression (LDS)..........................................................11

One Touch Calibration (One Touch Cal)......................................11

Audio ................................................................................................11

Contrast............................................................................................11

Sensor Type......................................................................................12

Membrane Type...............................................................................13

Auto Stable .......................................................................................14

DO Units ..........................................................................................14

Temperature Units...........................................................................15

Pressure Units ..................................................................................15

Language...........................................................................................15

Auto Shutoff.....................................................................................15

Resetting the System Setup Menu to Factory Default ...................16

Exiting the System Setup Menu ......................................................17

Calibration ................................................................................................17

Temperature.....................................................................................17

Barometer.........................................................................................17

Dissolved Oxygen.............................................................................18

Salinity Compensation Calibration ................................................21

Taking Measurements ..............................................................................22

Saving and Viewing data...........................................................................22

Saving Data.......................................................................................22

Viewing and Erasing Saved Data – Data mode ..............................23

Principles of Operation ............................................................................25

Care, Maintenance, and Storage ..............................................................26

General Maintenance.......................................................................26

Sensor Maintenance.........................................................................28

Sensor Storage..................................................................................32

Troubleshooting........................................................................................33

Specifications.............................................................................................35

Accessories/Part Numbers........................................................................36

Declaration of Conformity.......................................................................37

Recycling....................................................................................................38

Contact Information.................................................................................39

Ordering & Technical Support........................................................39

Service Information .........................................................................39

WARRANTY

The YSI Pro20 Instrument is warranted for three (3) years from date of purchase by the end user against defects in materials and workmanship, exclusive of batteries and any damaged caused by defective batteries. Pro20 cables are warranted for two (2) years from date of purchase by the end user against defects in material and workmanship. Pro20 Polarographic sensors are warranted for one (1) year and Galvanic sensors are warranted for six (6) months from date of purchase by the end user against defects in material and workmanship. Pro20 instruments, cables & probes are warranted for 90 days from date of purchase by the end user against defects in material and workmanship when purchased by rental agencies for rental purposes. Within the warranty period, YSI will repair or replace, at its sole discretion, free of charge, any product that YSI determines to be covered by this warranty.

To exercise this warranty, call your local YSI representative, or contact YSI Customer Service in Yellow Springs, Ohio at +1 937 767-7241, 800-897-4151 or visit www.ysi.com (Support tab). Send the product and proof of purchase, transportation prepaid, to the Authorized Service Center selected by YSI. Repair or replacement will be made and the product returned, transportation prepaid. Repaired or replaced products are warranted for the balance of the original warranty period, or at least 90 days from date of repair or replacement.

LIMITATION OF WARRANTY

This Warranty does not apply to any YSI product damage or failure caused by:

1) failure to install, operate or use the product in accordance with YSI's written instructions;

2) abuse or misuse of the product;

3) failure to maintain the product in accordance with YSI's written instructions or standard industry procedure;

4) any improper repairs to the product;

5) use by you of defective or improper components or parts in servicing or repairing the product;

6) modification of the product in any way not expressly authorized by YSI.

THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. YSI's LIABILITY UNDER THIS WARRANTY IS LIMITED TO REPAIR OR REPLACEMENT OF THE PRODUCT, AND THIS SHALL BE YOUR SOLE AND EXCLUSIVE REMEDY FOR ANY DEFECTIVE PRODUCT COVERED BY THIS WARRANTY. IN NO EVENT SHALL YSI BE LIABLE FOR ANY SPECIAL, INDIRECT, INCIDENTAL OR CONSEQUENTIAL DAMAGES RESULTING FROM ANY DEFECTIVE PRODUCT COVERED BY THIS WARRANTY.

INTRODUCTION

Thank you for purchasing the YSI Pro20, an instrument from the YSI Professional

Series product family. The Pro20 features an impact resistant and waterproof (IP-

67) case, backlit display, user-selectable sensor options, internal barometer, and a rugged, rubber over-mold case.

The Pro20 provides valuable instructions and prompts near the bottom of the display that will guide you through operation and use. However, reading the entire manual is recommended for a better understanding of the Pro20’s features.

The Pro20 can not communicate to a PC via a Pro Plus

communications saddle. Connecting the Pro20 to a

communication saddle may cause erratic instrument behavior.

GETTING STARTED

INITIAL INSPECTION

Carefully unpack the instrument and accessories and inspect for damage. Compare received parts with materials listed on the packing list. If any parts or materials are missing or damaged, contact YSI Customer Service at 800-897-4151 (+1-937-767-7241) or the Authorized YSI distributor from whom the instrument was purchased.

BATTERY INSTALLATION

This instrument requires 2 alkaline C-cell batteries. Under normal conditions, battery life is approximately 400 hours at room temperature without using the

back light. A battery symbol display to indicate low batteries when approximately 1 hour of battery life remains.

will blink in the lower, left corner of the

To install or replace the batteries:

1) Turn the instrument off and flip over to view the battery cover on the back.

2) Unscrew the four captive battery cover screws.

3) Remove the battery cover, and remove the old batteries if necessary.

4) Install the new batteries, ensuring correct polarity alignment (Figure 1).

5) Place the battery cover on the back of the instrument and tighten the four screws. Do NOT over-tighten.

Figure 1, Pro20 with battery cover removed. Note battery symbols indicating polarities.

The waterproof instrument case is sealed at the factory and is not to

be opened, except by authorized service technicians. Do not

KEYPAD

attempt to separate the two halves of the instrument case as this

may damage the instrument, break the waterproof seal, and will

void the warranty.

Figure 2, keypad

Number Key Description

Press and hold for 3 seconds to calibrate.

Opens Calibrate menu from the run screen if One

Use to navigate through menus, to navigate through

box options at the bottom of the run screen, and to

Press once to turn instrument on. Press a second

time to turn backlight on. Press a third time to turn

backlight off. Press and hold for 3 seconds to turn

Use to enter the System Setup menu from the run

Initiates One Touch Calibration.

Touch Calibration is disabled.

increase numeric inputs.

Press to confirm entries and selections.

Calibrate

Up Arrow

Power and Backlight

instrument off.

screen.

Enter

Use to navigate through menus, to navigate through

box options at the bottom of the run screen, and to

CONNECTING THE SENSOR AND CABLE

CONNECTING THE SENSOR

“Sensor” refers to the removable portion or electrode sensing portion of the cable assembly, i.e. the dissolved oxygen sensor. “Bulkhead” refers to the portion of the cable with the single-pin connector (Figure 3).

Down Arrow

decrease numeric inputs.

The Pro20 has two compatible sensors for use with a field cable:

Polarographic – This sensor has a black sensor body and is engraved with the model number 2003. Polarographic will be abbreviated Polaro in the instrument.

Galvanic – This sensor has a grey sensor body and is engraved with the model number 2002.

For information about the differences on the two sensor types, see Sensor Type in the System Setup menu section and/or the Principles of Operation section of this manual.

If using a ProBOD sensor/cable assembly, there is no need to install a sensor because it has a built in Polarographic dissolved oxygen sensor.

Before installing either sensor or connecting the cable to the

instrument, the Sensor Type must be configured for the sensor being

installed/connected. Failure to do this may result in damage not

covered under warranty. The instrument will step you through this

setup the first time it is powered on. See the System Setup menu section of this manual for instructions on configuring the Sensor

Type after the first power on.

1) Ensure both the sensor connector and sensor port on the cable are clean and dry.

2) Grasp the sensor with one hand and the cable bulkhead in the other.

3) Push the sensor into the connector on the cable until it is firmly seated and only 1 o-ring is visible. Failure to properly seat the sensor may result in damage.

4) Twist the sensor clockwise to engage threads and finger tighten. Do NOT use a tool. This connection is water-tight.

For more detailed instructions, please refer to the sensor installation sheet that is included with each sensor.

Bulkhead

Figure 3

Sensor

CONNECTING THE CABLE

The Pro20 is designed for field and laboratory use. It is compatible with two different cable options:

1) The field rugged cable is available in standard lengths of 1, 4, 10, 20, 30, and 100 meters with special lengths available between 30 and 100 meters. This cable has a built in temperature sensor and includes a port for the dissolved oxygen sensor.

2) The ProBOD is a 1 meter probe/cable assembly with built in Polarographic dissolved oxygen and temperature sensors. It has an AC powered motor for sample stirring and is designed to fit into a 300 ml BOD bottle.

To connect the cable, align the keys in the cable connector to the slots in the instrument connector. Push together firmly and then twist the outer ring until it locks into place (Figure 4). This connection is water-proof.

When disconnected, the sensor and cable’s sensor connectors are

NOT water-proof. Do not submerge the cable without a sensor

installed. When disconnected, the cable’s instrument connector and

the connector on the instrument maintain a waterproof, IP-67

Figure 4, Note the keyed connector.

rating.

MEMBRANE INSTALLATION

The dissolved oxygen sensor is shipped with a dry, protective red cap that will need to be removed before using. It is very important to put a new membrane with electrolyte solution on the sensor after removing the red cap.

Prepare the membrane solution according to the instructions on the bottle. After mixing, allow the solution to sit for 1 hour. This will help prevent air bubbles from later developing under the membrane. Ensure you are using the correct electrolyte solution for the correct sensor. Galvanic sensors utilize electrolyte with a light blue label and Polarographic sensors utilize electrolyte with a white label. The Dissolved Oxygen sensor is supplied with cap membranes specific to the sensor type ordered (Polarographic or Galvanic). 5913 and 5914 membrane kits are for Galvanic sensors and the 5908 and 5909 membrane kits are for Polarographic sensors.

Remove and discard or save the red protective cap. Thoroughly rinse the sensor tip with distilled or deionized water. Fill the cap membrane 3⁄4 full of electrolyte solution, then tap the cap with a finger to release any trapped air. Be careful not to touch the membrane portion of the cap. Thread the membrane cap onto the sensor, moderately tight. Do not use a tool. It’s typical for some of the electrolyte solution to spill over. It is best to allow the new cap to remain on a new sensor

overnight before trying to calibrate. For detailed instructions on changing a membrane cap, see the Care, Maintenance, and Storage section of this manual.

BACKLIGHT

Once the instrument is on, pressing power/backlight key will turn on the display backlight. The backlight will remain on until the key is pressed again or after two minutes of not pressing any key on the keypad.

POWERING OFF

To turn the instrument off, press and hold the power/backlight key for three seconds.

RUN SCREEN

Press the power/backlight key to turn the instrument on. The instrument will run through a self test and briefly display a splash screen with system information before displaying the main run screen (Figure 5). The first time the Pro20 is

turned on, it will step through language, sensor, and membrane selections; see the First Power On section of this manual for more information.

8.04 mg/L

95.9 %

Figure 5, example of main run screen with Save highlighted.

NAVIGATION

The up and down arrow keys allow you to navigate through the functions of the Pro20.

NAVIGATING IN THE RUN SCREEN

When in the run screen, the up and down arrow keys will move the highlighted box along the bottom options. Once a box is highlighted, press enter

to access the highlighted option.

730.3

mmHg

24.2 °C

0.0

ppt



SAVE



DATA

Description of run screen box functions from left to right:

Option Description

Barometer reading Highlight and press enter to calibrate the barometer

Salinity compensation value



SAVE



DATA

NAVIGATING IN THE SYSTEM SETUP MENU

When in the System Setup menu, the up and down arrow keys will move the highlighted bar up and down the system setup options. See the System Setup menu section of this manual for more information about these options.

FIRST POWER ON

The instrument will step through an initial configuration when powered on for the first time. This will set the language, sensor, and membrane options. Use the up or down arrow keys to highlight the appropriate language, sensor, and membrane, then press enter to confirm (Figures 6, 7, and 8). The Sensor Type must be configured for the sensor installed. Failure to do this may result in damage not covered under warranty. If an incorrect option is selected, it may be changed in the System Setup menu.

Select Language:

English

Français



Español



Deutsch



Use ST to select Language Press  to confirm

Figure 6, Language selection

Highlight and press enter to adjust salinity compensation value

Highlight and press enter to save current data to memory

Highlight and press enter to view and/or erase saved data

Select Sensor Type:

Polaro (black)

Galvanic (grey)



Use ST to select sensor type Press  to confirm

Figure 7, Sensor selection

Select Membrane Type:

71.25 (Yellow)  2.0 (Blue)

Use ST to select membrane Press  to confirm

Figure 8, Membrane selection

After selecting a language, sensor, and membrane, the run screen will appear. The next time the instrument is powered up the run screen will appear immediately after the self check. If the sensor type or membrane type is changed, ensure that it updated in the System Setup menu.

SYSTEM SETUP MENU

Press the menu key to access the following System Setup functions.

The System Setup menu contains multiple screens which are notated as ‘pages’. The current page is indicated on the display, figure 9.

DO LOCAL%

DO Local% can be enabled or disabled by using the up or down arrow keys to highlight it and then pressing enter. An ‘X’ in the box next to DO Local% indicates it is enabled (Figure 9).

When DO Local% is enabled, DO% values will be expressed as %L on the run screen.

DO Local% allows for localized dissolved oxygen measurements. This sets the DO% calibration value to 100% regardless of the altitude or barometric pressure. When DO Local% is enabled, the Pro20 will factor in the barometric pressure on each measurement. For example, if the barometric pressure changes, the DO %L reading would remain constant in air-saturated water or in water-saturated air. Local DO is ideal for EU compliance.

7 DO Local%  LDS  One Touch Cal  Audio

Contrast Sensor Type Membrane Type

page 1 of 2

ESC

Exit

Figure 9, DO %Local is enabled.

Reset

LAST DIGIT SUPPRESSION (LDS)

Last Digit Suppression (LDS) can be enabled or disabled by using the up or down arrow keys to highlight it and pressing enter

indicates it is enabled.

LDS rounds the DO value to the nearest tenth; i.e. 8.25 mg/L becomes 8.3 mg/L. LDS is automatically disabled during calibrations.

ONE TOUCH CALIBRATION (ONE TOUCH CAL)

One Touch Calibration can be enabled or disabled by using the up or down arrow keys to highlight One Touch Cal and pressing enter

One Touch indicates it is enabled.

. An ‘X’ in the box next to LDS

. An ‘X’ in the box next to

When One Touch Cal is enabled, press and hold the calibration seconds while in the run screen to calibrate Dissolved Oxygen to the barometer reading and salinity correction value. For more information on One Touch Calibration, see the Calibration section of this manual.

AUDIO

Audio can be enabled or disabled by using the up or down arrow keys to highlight Audio and pressing enter

to Audio.

When Audio is enabled, the Pro20 will beep twice to indicate stability when Auto Stable is enabled. The instrument will also beep when a key is pressed. When Audio is disabled, the Pro20 will not beep.

CONTRAST

To adjust the display Contrast, use the up or down arrow keys to highlight

Contrast, then press enter

contrast. The up arrow will darken the contrast and the down arrow will lighten the contrast. After adjusting the contrast, press enter to save and exit the Contrast adjustment option.

. When enabled, there will be an ‘X’ in the box next

. Next, use the up or down arrow keys to adjust the

key for 3

EMERGENCY CONTRAST ADJUSTMENT

If necessary, there is an alternate method of adjusting the contrast. To adjust the contrast, press and hold the menu key, then press the up arrow key to darken the contrast or press the down arrow key to lighten the contrast.

SENSOR TYPE

The instrument’s Sensor Type must be configured for the sensor

Sensor Type sets the type of oxygen sensor being used; either Polarographic (black) or Galvanic (grey).

installed. Failure to do this may result in damage not covered

under warranty. If you observe readings very close to 0 or

extremely high readings, i.e. 600%, your Sensor Type setting may

be set incorrectly.

Use the up or down arrow keys to highlight Sensor Type, then press enter open a submenu. Highlight the sensor type corresponding to the sensor installed on the cable and press enter to confirm. The enabled sensor type will have an ‘X’ in the box next to it. Use the down arrow key to highlight the ESC – Exit, then press enter to save changes and to close the sensor submenu.

If using a ProBOD sensor/cable assembly, the sensor type should be set to polarographic.

The Pro20 has two compatible sensors for use with a field cable:

Polarographic – This sensor has a black sensor body and is engraved with the model number 2003. Polarographic will be abbreviated Polaro in the instrument.

Galvanic – This sensor has a grey sensor body and is engraved with the model number 2002.

In terms of physical configuration, membrane material, and general performance, YSI Professional Series Galvanic dissolved oxygen sensors are exactly like the Professional Series Polarographic sensors. The advantage of using Galvanic sensors is convenience. Galvanic sensors provide for an instant-on sensor without the need for warm-up time but this affects the life of the sensor. Polarographic sensors last longer and have a longer warranty but require a 5-15 minute warm-up time before use or calibration.

MEMBRANE TYPE

Membrane Type sets the type of membrane used on the dissolved oxygen sensor; either 1.25 PE (Yellow) or 2.0 PE (blue). Use the up or down arrow keys to

highlight Membrane Type and press enter

to open the membrane submenu. Highlight the membrane type corresponding to the membrane installed on the sensor and press enter to confirm. The enabled membrane type will have an ‘X’ in the box next to it. Use the down arrow key to highlight the ESC – Exit box and press enter to save changes and to close the membrane submenu.

The dissolved oxygen sensor is supplied with membranes specific to the sensor type ordered and are color coded as described in the following tables.

Galvanic Membrane Kits

Item Color Material Description

5913 Yellow 1.25 mil polyethylene Faster response time and less flow

dependence than traditional Teflon® membranes

5914 Blue 2.0 mil polyethylene Less flow dependence than 1.25 mil

but somewhat slower response

Polarographic Membrane Kits

Item Color Material Description

5908 Yellow 1.25 mil polyethylene Faster response time and less flow

dependence than traditional Teflon® membranes

5909 Blue 2.0 mil polyethylene Less flow dependence than 1.25 mil

but somewhat slower response

Selecting a Dissolved Oxygen Membrane

Membrane Type Flow Dependence After 4 Minutes Typical Response Time

to 95%

5913, 5908 - Yellow 25% 8 seconds

5914, 5909 – Blue 18% 17 seconds

AUTO STABLE

Auto Stable utilizes preset values to indicate when a reading is stable. The preset values are adjustable in the System Setup menu. The user can input a % change in dissolved oxygen readings (0.0 to 1.9) over ‘x’ amount of time in seconds (3-19).

Highlight Auto Stable and press enter

to expand the submenu. Use the up or down arrow keys to highlight the DO% Change or seconds (secs) input field, then press enter to make the highlighted field adjustable. Use the up and down arrow keys to adjust the selected value, then press enter to confirm changes. Once you have confirmed any changes, highlight the ESC-Exit box and press enter to close the Auto Stable submenu.

To disable Auto Stable, set the DO% Change input to 0.0.

When Auto Stable is enabled, a

will display next to the dissolved oxygen

value on the run screen and blink during stabilization. When the dissolved oxygen value has stabilized based on the Auto Stable settings, the

will display steadily

and the instrument will beep twice if Audio is turned on.

DO UNITS

Highlight DO Units and press enter to open a submenu that will allow you to select the dissolved oxygen units displayed on the run screen. Highlight the desired unit(s) and press enter to enable or disable. An enabled dissolved oxygen unit will have an ‘X’ in the box next to it. Highlight the ESC-Exit box and press enter to save any changes and to close the DO units submenu.

There are three options for displaying dissolved oxygen:

 mg/L will show DO readings in milligrams per liter on a scale

from 0 to 50 mg/L.

 ppm (parts per million) is equivalent to mg/L and will show the

DO reading on a scale from 0 to 50 ppm.

 % will show DO readings in a percent scale from 0 to 500%.

This value will be expressed %L when DO Local% is enabled.

Both % or %L and mg/L or ppm can be displayed simultaneously on the screen.

TEMPERATURE UNITS

Highlight Temperature Units and press enter to open a submenu that will allow you to change the temperature units displayed in the run screen. Highlight the desired unit (Celsius or Fahrenheit) and press enter to enable. The enabled temperature unit will have an ‘X’ in the box next to it. Only one unit may be enabled at a time. Highlight the ESC-Exit box and press enter to save any changes and to close the Temperature Units submenu.

PRESSURE UNITS

Highlight Pressure Units and press enter to open a submenu that will allow you to change the units displayed on the run screen. Highlight the desired unit (mmHg, inHg, mbar, psi, or kPa) and press enter to enable. The enabled pressure unit will have an ‘X’ in the box next to it. Only one unit may be enabled at a time. Highlight the ESC-Exit box and press enter to save any changes and to close the Pressure Units submenu.

LANGUAGE

Highlight Language and press Enter to open a submenu that will allow you to change the language. Highlight the desired language (English, Spanish, German, or French) and press enter to enable. The enabled language will have an ‘X’ in the box next to it. Highlight ESC-Exit box and press enter to save any changes and to close the Language submenu.

The text in the boxes along the bottom of the run screen will always be displayed in English regardless of the language enabled in the System Setup menu.

AUTO SHUTOFF

Auto Shutoff allows you to set the instrument to turn off automatically after a period of time. Use the up or down arrow keys to highlight Auto Shutoff, then press enter to open the submenu. Press enter while the minute field is highlighted to make it adjustable. Next, use the up and down arrow keys to adjust the shut off time from 0 to 60 minutes. Press enter to confirm and save the new shutoff time. Highlight ESC-Exit box, then press enter to close the Auto Shutoff submenu.

To disable Auto Shutoff, set the Time in Minutes to 0 (zero).

RESETTING THE SYSTEM SETUP MENU TO FACTORY DEFAULT

To reset the Pro20 settings to factory default, press the down arrow key until the Reset - 4 box is highlighted, then press enter. The instrument will ask you to confirm the reset. Highlight Yes and press enter to continue with the reset or highlight No and press enter to cancel the reset. A Factory Reset will not affect data saved in the unit’s memory.

The following will be set in the Pro20 after performing a factory reset:

Parameter Reset Defaults

Temperature Units

Dissolved Oxygen Units mg/L and %

Pressure Units mmHg

Dissolved Oxygen Sensor Type Last Setting Confirmed

Membrane Type Last Setting Confirmed

Salinity Compensation Value 0.0 ppt

DO Local% Off

One Touch Cal On

Display Contrast Set to mid range

Auto Shutoff 30 minutes

Auto Stable Off (0.0 % Change and 10 secs)

LDS (Last Digit Suppression) Off

Audio On

Language English

Dissolved Oxygen Calibration Reset to factory default, 100% for

enabled membrane and sensor*

Barometer Calibration Reset to factory default*

*It is recommended to perform a barometer and dissolved oxygen calibration

erforming a reset.

after

EXITING THE SYSTEM SETUP MENU

To exit the System Setup menu, press the down arrow key until the ESC - Exit box is highlighted, then press enter

to return to the run screen.

CALIBRATION

TEMPERATURE

All cable assemblies have built-in, temperature sensors. Temperature calibration is not required nor is it available.

BAROMETER

The barometer in the Pro20 is calibrated at the factory. The barometer reading must be accurate to ensure accurate % calibrations and DO readings. If your barometer requires an adjustment, use the up or down arrow keys to highlight the barometer box on the run screen, then press enter. Next, use the up or down arrow keys to adjust the barometer reading to the local, true barometric pressure. Continually depress the up or down arrow keys to change the barometer value more rapidly. Press enter to confirm and save the barometer adjustment.

Do not use a barometer value that is corrected to sea level.

Laboratory barometer readings are usually “true” (uncorrected)

True BP = [Corrected BP] – [2.5 * (Local Altitude in ft above sea level/100)]

values of air pressure and can be used “as is” for barometer calibration. Weather service readings are usually not “true”, i.e., they are corrected to sea level, and therefore cannot be used until

they are “uncorrected”. An approximate formula for this

“uncorrection” is:

Although the barometer range is 400.0 to 999.9 mmHg, you will be

unable to adjust the value across the entire range. The barometer is

very accurate and the instrument will not allow you to adjust the value drastically beyond what it is measuring during calibration.

DISSOLVED OXYGEN

The Pro20 can be easily calibrated with the press of one key by enabling One Touch Cal in the System Setup menu and following the One Touch Calibration procedure.

Ensure the barometer is reading accurately before performing a One Touch Calibration, DO %, or DO Local% calibration. These calibration procedures use the barometer reading during calibration. If the barometer reading is erroneous during a calibration, your dissolved oxygen values will be inaccurate.

It is not necessary to calibrate in both % and mg/L or ppm.

ONE TOUCH CALIBRATION

Perform this calibration procedure when One Touch Cal is enabled in the System Setup menu.

If using a field cable, install the sensor guard onto the probe. Moisten the sponge in the grey calibration/storage sleeve with a small amount of water and install it over the sensor guard. The sleeve should be moist, but should not have excess water that could cause water droplets to get on the membrane. The storage sleeve ensures venting to the atmosphere.

Calibrating in % will simultaneously calibrate mg/L and ppm and

vice versa. YSI recommends calibrating dissolved oxygen in % for

both ease and accuracy.

If using the ProBOD sensor/cable assembly, place the probe in 300 ml BOD bottle with a small amount of water (1/8 inch or 0.3 cm). The dissolved oxygen and temperature sensors should not be immersed in water.

If the calibration/storage sleeve is not available, substitute with a chamber of 100% relative humidity, vented to the atmosphere (not completely sealed).

Power the instrument on and wait approximately 5 to 15 minutes for the storage chamber to become completely saturated and to allow the sensor to stabilize if using a Polarographic sensor. If using a Galvanic sensor, wait approximately 5 to 10 minutes for the chamber to become completely saturated. Auto Shutoff time should be disabled or set to at least 20 minutes, see System Setup menu for more information on adjusting the Auto Shutoff.

Ensure the barometer reading is accurate. If necessary, perform a barometer calibration.

Press and hold the Calibrate Calibrating %DO on the display and automatically calibrate the sensor to the barometer and salinity correction values. This may take up to 2 minutes depending on the age of the sensor and membrane. You may press the Cal key at this time to cancel the calibration.

Calibration Successful will display for a few seconds to indicate a successful calibration and then the instrument will return to the run screen.

If the calibration is unsuccessful, an error message will display on the screen. Press the Cal key to exit the calibration error message and return to the run screen. See the Troubleshooting guide for possible solutions.

CALIBRATING IN PERCENT (DO %)

Perform this calibration procedure when One Touch Cal is disabled in the System Setup menu.

Prepare a 100% humid environment for the sensor as described in the previous calibration section.

key for 3 seconds. The Pro20 will indicate

Ensure the barometer reading is accurate. If necessary, perform a barometer calibration.

Press and hold the Calibrate The Pro20 will display the current DO% and temperature readings along with the % calibration value. The % calibration value is based on the barometer reading.

Wait at least 3 seconds, then, once the DO% and temperature readings are stable, press enter to complete the calibration. Or, press the Cal key to cancel the calibration.

Calibration Successful will display for a few seconds to indicate a successful calibration and then the instrument will return to the run screen.

key for 3 seconds. Highlight % and press enter.

CALIBRATING IN PERCENT (DO LOCAL% ENABLED)

Perform this calibration procedure when DO Local% is enabled in the System Setup menu.

Prepare a 100% humid environment for the sensor as described in the One Touch Calibration section.

Ensure the barometer reading is accurate. If necessary, perform a barometer calibration.

Press and hold the Calibrate

key for 3 seconds. %Local will be automatically

highlight, press enter. The Pro20 will display the current DO% and temperature readings along with the % calibration value. The % calibration value will always be 100% for DO Local%.

Wait at least 3 seconds, then, once the DO% and temperature readings are stable, press enter to complete the calibration. Or, press the Cal key to cancel the calibration.

Calibration Successful will display for a few seconds to indicate a successful calibration and then the instrument will return to the run screen.

CALIBRATING IN MG/L

Power the instrument on and place the sensor into a sample that has been titrated to determine the dissolved oxygen concentration. Continuously stir or move the probe through the sample at a rate of at least ½ foot per second (16 cm per second) during the entire calibration process. A stir plate may be helpful in this calibration.

Allow the dissolved oxygen and temperature readings to stabilize. This may take 5 to 15 minutes, depending on the age of the instrument, type of sensor, and condition of the sensor.

Press the Calibrate

Use the up and down arrow keys to adjust the mg/L reading to the value of the titrated sample. Press enter to confirm the value and calibrate or press the Cal key to cancel the calibration.

Calibration Successful will display for a few seconds to indicate a successful calibration and then the instrument will return to the run screen.

SALINITY COMPENSATION CALIBRATION

The Pro20 uses a user inputted salinity value in ppt (parts per thousands) to compensate dissolved oxygen mg/L values. The salinity compensation value entered in the Pro20 should be the salinity value of the water you are testing.

To adjust the salinity compensation value, use the up or down arrow keys to highlight the salinity box on the run screen, and then press enter (Figure 10). Next, use the up or down arrow keys to adjust the salinity compensation value to the salinity of the water you are testing. You may enter a value between 0.0 and

70.0 parts per thousand (ppt). Press enter to confirm and to save the new salinity compensation value.

key. Highlight mg/L and press enter.

The salinity compensation value can be adjusted any time without the need to recalibrate dissolved oxygen.

6.58 mg/L

95.9 %

24.2 °C

729.7

mmHg

35.0

ppt

Figure 10, Salinity box highlighted.



SAVE



DATA

TAKING MEASUREMENTS

Before taking measurements, be sure the instrument has been calibrated to ensure the most accurate readings. Turn the instrument on and wait 5-15 minutes if using a polarographic sensor. If using a field cable/sensor, install the sensor guard to protect the sensor and membrane. Place the probe in the sample to be measured and give the probe a quick shake to release any air bubbles. Allow the temperature readings to stabilize. Next, stir the probe in the sample to overcome the stirring dependence of the dissolved oxygen sensor. You must provide at least 6 inches (16 cm) per second of water movement. Once the values plateau and stabilize you may record the measurement and/or store the data set. The dissolved oxygen reading will drop over time if stirring is ceased. If placing the DO sensor into a stream or fast flowing waters it is best to place it perpendicular to the flow and NOT facing into the flow.

If using the DO sensor in an aeration tank/basin it is helpful to make sure bubbles do not burst on the membrane. This may cause unstable readings to occur. You should be able to prevent this by pointing the sensor upwards so it’s facing the sky and twist tying, zip tying, or rubber banding the bulkhead to the cable. Essentially making a simple curve to the cable without bending or breaking the cable will allow you to lower the sensor into the aeration tank while the sensor points skyward and the bubbles are no longer bursting on the membrane surface.

SAVING AND VIEWING DATA

The Pro20 can store 50 data sets in non-volatile memory for later viewing. A data set includes the values currently on the display, i.e. temperature in Celsius or Fahrenheit and dissolved oxygen in % and/or mg/L or ppm. Each data point is referenced with a data set number, 01 through 50.

SAVING DATA

From the run screen, use the up or down arrow keys to highlight the Save box and press enter to save the current readings. The instrument will indicate the data set is saved and display the saved data set’s number (Figure 11).

The Pro20 can not communicate to a PC via a Pro Plus

communications saddle. Connecting the Pro20 to a

communication saddle may cause erratic instrument behavior.

The instrument will display ‘Memory Full’ if all 50 data sets have been saved and you attempt to save another data set.

VIEWING AND ERASING SAVED DATA – DATA MODE

Data mode allows you to view and erase saved data. From the run screen, use the up or down arrow keys to highlight Data and press enter to access data mode. Note that the function boxes at the bottom of the display are different in data mode (Figure 12).

6.58 mg/L

95.9 %

24.2 °C

Data set 01 saved

729.7

mmHg

35.0

ppt

Figure 11, Data set saved



SAVE



DATA

6.58 mg/L

95.9 %

24.2

Data set 01

ESC



Exit

ure 12, Data mode

Erase

°C



DATA

VIEWING DATA

Once in data mode, use the up and down arrow keys to view saved data sets in sequential order or press enter to access the bottom functions. After accessing the bottom functions, highlight the Data box and press enter to regain access to viewing data. The data set that is displayed will be indicated by the data set number, 01 through 50.

ERASING DATA

While viewing saved data, press the enter key to access the function boxes at the bottom of the display. Next, use the up or down arrow keys to highlight Erase, then press enter. The instrument will give you the option to erase one or all data sets (Figure 13).

Erase Data Set

Erase All Sets

Use the up or down arrow key to select Erase Data Set, Erase All Sets, or the ESCExit function box, then press enter to confirm.

Select ESC-Exit and press enter to exit erase mode without erasing any data.

Select Erase Data Set and press enter to erase the data set that was displayed before entering erase mode. For example, if data set 12 was displayed before entering erase mode, and Erase Data Set is selected, Data Set 12 will be erased from memory and the sets AFTER that number will move up to keep them sequential. So, if there were 15 records and number 12 is erased then 13 becomes 12, 14 becomes 13, and 15 becomes 14. The instrument will return to data mode after erasing one data set.

Select Erase All Data Sets and press enter to clear the Pro20 memory and return to data mode.

ESC

Exit

Figure 13, erasing data

EXITING DATA MODE

While in Data mode, press enter to access the bottom functions. Next, highlight the ESC-Exit box and press enter to return to the run screen.

PRINCIPLES OF OPERATION

The polarographic sensor consists of a silver body as the anode and a circular gold cathode embedded in the end. The galvanic sensor consists of a zinc anode and silver cathode. The polarographic sensor requires an applied voltage for operation while the galvanic sensor electrode potentials are dissimilar enough to reduce oxygen with applied voltage.

Both sensors have a thin semi-permeable membrane, stretched over the sensor, which isolates the electrodes from the environment, while allowing gases to enter. In operation, this end of the sensor is filled with a solution of electrolyte containing a small amount of surfactant to improve wetting action.

When a polarizing voltage is applied to the polarographic sensor electrodes, oxygen that has passed through the membrane reacts at the cathode causing a current to flow. This same reaction takes place with the galvanic sensor without the applied voltage.

For both polarographic and galvanic DO sensors, oxygen diffuses through the membrane at a rate proportional to the oxygen pressure difference across it. Since oxygen is rapidly consumed at the cathode, it can be assumed that the oxygen pressure inside the membrane is zero. Hence, the amount of oxygen diffusing through the membrane is proportional to the absolute pressure of oxygen outside the membrane. If the oxygen pressure increases, more oxygen diffuses through the membrane and more current flows through the sensor. A lower pressure results in less current.

CARE, MAINTENANCE, AND STORAGE

This section describes the proper procedures for care, maintenance and storage of the sensors. The goal is to maximize their lifetime and minimize down-time associated with improper sensor usage.

GENERAL MAINTENANCE

GENERAL MAINTENANCE - O-RINGS

The instrument utilizes o-rings as seals to prevent water from entering the battery compartment and the sensor ports. Following the recommended procedures will help keep your instrument functioning properly.

If the o-rings and sealing surfaces are not maintained properly, it is possible that water can enter the battery compartment and/or sensor ports of the instrument. If water enters these areas, it can severely damage the battery terminals or sensor ports causing loss of battery power, false readings and corrosion to the sensors or battery terminals. Therefore, when the battery compartment lid is removed, the oring that provides the seal should be carefully inspected for contamination (e.g. debris, grit, etc.) and cleaned if necessary.

The same inspection should be made of the o-rings associated with the dissolved oxygen sensor connector when it is removed. If no dirt or damage to the o-rings is evident, then they should be lightly greased without removal from their groove. However, if there is any indication of damage, the o-ring should be replaced with an identical o-ring. At the time of o-ring replacement, the entire o-ring assembly should be cleaned.

To r em ov e t he o -r i ngs :

Use a small, flat-bladed screwdriver or similar blunt-tipped tool to remove the oring from its groove. Check the o-ring and the groove for any excess grease or contamination. If contamination is evident, clean the o-ring and nearby plastic parts with lens cleaning tissue or equivalent lint-free cloth. Alcohol can be used to clean the plastic parts, but use only water and mild detergent on the o-ring itself. Also, inspect the o-rings for nicks and imperfections.

+ 154 hidden pages

YSI Pro20 Operating Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

INITIAL INSPECTION

BATTERY INSTALLATION

KEYPAD

CONNECTING THE SENSOR AND CABLE

CONNECTING THE SENSOR

CONNECTING THE CABLE

MEMBRANE INSTALLATION

BACKLIGHT

POWERING OFF

RUN SCREEN

NAVIGATION

NAVIGATING IN THE RUN SCREEN

NAVIGATING IN THE SYSTEM SETUP MENU

FIRST POWER ON

DO LOCAL%

LAST DIGIT SUPPRESSION (LDS)

ONE TOUCH CALIBRATION (ONE TOUCH CAL)

AUDIO

CONTRAST

EMERGENCY CONTRAST ADJUSTMENT

SENSOR TYPE

MEMBRANE TYPE

AUTO STABLE

DO UNITS

TEMPERATURE UNITS

PRESSURE UNITS

LANGUAGE

AUTO SHUTOFF

RESETTING THE SYSTEM SETUP MENU TO FACTORY DEFAULT

EXITING THE SYSTEM SETUP MENU

TEMPERATURE

BAROMETER

DISSOLVED OXYGEN

ONE TOUCH CALIBRATION

CALIBRATING IN PERCENT (DO %)

CALIBRATING IN PERCENT (DO LOCAL% ENABLED)

CALIBRATING IN MG/L

SALINITY COMPENSATION CALIBRATION

SAVING DATA

VIEWING AND ERASING SAVED DATA – DATA MODE

VIEWING DATA

ERASING DATA

EXITING DATA MODE

GENERAL MAINTENANCE

GENERAL MAINTENANCE - O-RINGS

To r em ov e t he o -r i ngs :