Teledyne D series, 30D, 100DX, 65D, 260D Installation And Operation Manual

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D-Series Pumps

Installation and Operation Guide

Foreword

This instruction manual is designed to help you gain a thorough understanding of the operation of the equipment. Teledyne ISCO recommends that you read this manual completely before placing the equipment in service.

Although Teledyne ISCO designs reliability into all equipment, there is always the possibility of a malfunction. This manual may help in diagnosing and repairing the malfunction.

If a problem persists, call or e-mail Teledyne ISCO technical support for assistance. Simple difficulties can often be diagnosed over the phone. For faster service, please have your serial number ready.

If it is necessary to return the equipment to the factory for service, please follow the shipping instructions provided by technical support, including the use of the Return Material Authorization (RMA) specified. Be sure to include a note describing the malfunction. This will aid in the prompt repair and return of the equipment.

Teledyne ISCO welcomes suggestions that would improve the information presented in this manual or enhance the operation of the equipment itself.

Teledyne ISCO is continually improving its products and reserves the right to change product specifications, replacement parts, schematics, and instructions without notice.

Contact Information

Customer Service

Phone: (800) 228-4373 (USA, Canada, Mexico)

Fax: (402) 465-3022

Email: ISCO CSR@teledyne.com

Technical Support

Phone: Toll Free (800) 775-2965 (Syringe Pumps and Liquid Chromatography)

Email: IscoService@teledyne.com

Return equipment to: 4700 Superior Street, Lincoln, NE 68504-1398

Other Correspondence

Mail to: P.O. Box 82531, Lincoln, NE 68501-2531

Email: IscoInfo@teledyne.com

(402) 464-0231 (Outside North America)

EAR-Controlled Technology Subject to Restrictions Contained on the Cover Page.

Warnings and Cautions

The lightning flash and arrowhead within the triangle is a warning sign alerting you to “dangerous voltage” inside the product.

The exclamation point within the triangle is a warning sign alerting you to important instructions in this manual.

D Series Syringe Pumps

Warnings, Cautions, and Notices

Symboles de Sécurité

Ce symbole signale la présence d’un danger d’électrocution.

Ce symbole signale l’existence d’instructions importantes relatives au produit dans ce manuel.

D Series Syringe Pumps Warnings, Cautions, and Notices

Warnungen und Vorsichtshinweise

Der gepfeilte Blitz im Dreieck ist ein Warnzeichen, das Sie vor

„gefährlichen Spannungen“ im Inneren des Produkts warnt.

Das Ausrufezeichen in Dreieck ist ein Warnzeichen, das Sie darauf aufmerksam macht, daß wichtige Anleitungen zu diesem Handbuch gehören.

Advertencias y Precauciones

Esta señal alerta sobre la presencia de alto voltaje en el interior del producto.

Esta señal le advierte sobre la importancia de las instrucciones del manual que acompañan a este producto.

D Series Syringe Pumps

Warnings, Cautions, and Notices

“To prevent damaging the instrument or injuring yourself, it is

absolutely necessary that you understand everything in English, above all, technical terms, before operating the instrument. Otherwise, it is necessary for you to receive complete instruction from someone qualified who understands both the instrument and English very well.”

“Um eine Beschädigung des Gerätes oder eine Gefährdung des

Anwenders zu vermeiden ist es notwendig, daß dieser vollstandig die englische Sprache und die technischen Bezeichnungen beherrscht. Oder der Anwender muß von einer Person eingeübt werden, die bereits vorher dieses Gerät bedienst hat.”

“Pour empêcher dommage à l’instrument ou blesser vous-même, il faut

absolument que vous compreniez tout en anglais, surtout les termes techniques, avant d’actionner l’instrument. Autrement, il faut que vous receviez l’instruction parfaite d’une personne très compétente qui comprend bien les deux l’instrument et anglais.”

“Para prevenir cualquier daño en el instrumento o en el operador, es

necesario que el usuario comprenda perfectamente el lenguaje inglés y las términos técnicos intrínsecos, o bien ser formado por una persona que haya trabajado ya previamente con este instrumento.”

“For a forhindre skade på instrumentet eller operatøren er det

nødvendig at brukeren har full forståelse for det engelske sprak og tekniske uttrykk Ellers må brukeren få opplæring av en person, som kan engelsk, for instrumentet tas i bruk.”

“För att förhindra skade på instrumentet eller operatören, år det

nötvändigt att användaren har fullständiga kunskaper i det engelska språket och dess tekniska termer, eller utbildas av en person, som tidigare brukat instrumentet.”

“For at undgå skade på produktet eller på brugeren er det nødvendigt

at brugeren til fulde forstår det engelske sprog for at forstå den tekniske formulering i den engelske manual. I modsat fald skal brugeren modtage træning, inden apparatet tages I drift.”

iii

D Series Syringe Pumps Warnings, Cautions, and Notices

Laitteelle tai käyttäjälle aiheutuvien vahinkojen välttämiseksi on tärkeää, että käyttäjä hallitsee englannin kielen ja englantilaiset tekniset termit tai on saanut käyttöopastuksen englantia osaavalta henkilöltä.

“Per evitare danni allo struménto od incidenti all’operatore, é

necessario che l’utilizzatore abbia una completa conoscensza della lingua inglese oppure che venga istruita da una persona che abbia utilizzato precedentemente questo strumento.”

“Para impedir qualquer dano no aparelho ou ferimentos parao

operador, é necessario que o utilizador tenha um conhecimento completo da lingua inglesa e dos respectivos termos técnicos, ou seja, treinado por uma pessoa que tenha esse conhecimento, antes de operar com este aparelho.”

“‘

  ”

С цел да избегне повреда на апаратурата или нараняване на оператора е необходимо клиента добре да владее английски език и техчническата терминолгия, която е използувана в описанието или да бъде обучен от лице, което е вече работило с такъв апарат.

Figyelmeztetés! A készülék meghibásodásának valamint a kezelö

sérülésének megelözése érdekében a felhasználónak feltétlenül értenie kell az angol nyelvet, ezen belül a müszaki kifejezéseket, vagy pedig a használatba vételt megelözöen a készülék kezelésében már gyakarlott személy által történö betanitás szükséges!

D Series Syringe Pumps

Warnings, Cautions, and Notices

- EXPLOSION WARNING -

WARNING

Teledyne Isco D Series Pumps, SFX 2-10 and

SFX 220 Extractors are NOT EXPLOSION PROOF.

Teledyne Isco SFX System and D Series Syringe

Pump Safety Note when using a flammable fluid

The Teledyne Isco SFX system and syringe pumps must be placed within a properly operating vent hood (fume cupboard), when using ethane or any other flammable gas. Ensure that all SFE tubing connections are completely free of any gas leaks by performing the leak test using CO pump manual, Section 5 of the SFX 2-10 manual and Section 6 of the SFX 2-10, 220, and SFX 3560 manual). There must absolutely be NO gas leaks present before introducing the flammable gas. In a temperature-stable, leak-free system, the flow rate, as registered by the pump, should settle to a value below 0.01 ml/min after 15, minutes during a static extraction.

(detailed in Section 2 of the D Series

Important: When using a Teledyne Isco extractor (either SFX 2-10, SFX 220, SFX 3560) be absolutely sure the built-in venting fan (a brushless motor) is operating properly.

The Teledyne Isco D Series Syringe Pumps and the SFX units, use brush-type drive motors. Minor modifications to the pumps may render them safer, especially in the rare event of catastrophic piston seal failure. However, these modifications

will not make these pumps explosion proof.

•Remove the front and back cylinder covers located on the ball screw tower. This will allow any escaped gas to quickly dissipate away from the pump cylinder area and to reduce the amount entering the motor compartment.

•Seal the syringe pump motor compartment with tape and purge it with a continuous flow of nitrogen (N mulating an explosive mixture around the motor and relays.

•For further information, telephone Teledyne Isco at (800)775-2965, or fax (402)465-3085 to consult the Teledyne Isco Service Department.

USE THE Teledyne Isco SFE SYSTEM AND SYRINGE PUMPS IN THESE

POTENTIALLY HAZARDOUS APPLICATIONS AT YOUR OWN RISK!

) gas. This will also reduce the possibility of accu-

5-97

D Series Syringe Pumps Warnings, Cautions, and Notices

- AVERTISSEMENT D’EXPLOSION -

AVERTISSEMENT

Les pompes de Série ‘D’ Teledyne Isco et l’extracteur SFX 2-10,

SFX 220, et SFX 3560 ne sont pas à l’épreuve d’explosion!

Remarque de sûreté concernant l’usage d’un fluide inflammable avec les

pompes à seringue d’Isco Série ‘D’ et le système SFX.

Le système SFX et pompes à seringue Teledyne Isco doivent être placées à l'intérieur d’une hotte chimique fonctionnelle, quand vous utilisez éthane C2H6, ou tout autre gaz inflammable. Assurez-vous que toute la tuyauterie SFE est complètement scellée avec aucune fuite de gaz en exécutant une épreuve de fuite en utilisant du CO2. (Cette méthode est détaillée à la partie 2 du manuel de la pompe Série ‘D’et à la partie 2 du manuel SFX 2-10, SFX 220, et 3560.) Il faut absolument qu'il n'y ait aucune fuite de gaz avant d'introduire le gaz inflammable au système. À partir d’un système à température stable et sans aucune fuite de gaz, la valeur du flot qui est indiqué par la pompe, devrait se stabiliser à une valeur moins de 0,01 ml/min après 15 minutes, pendant une extraction statique.

Important: Quand vous utilisez un extracteur Teledyne Isco SFX 2-10, SFX 220, et 3560, soyez absolument certain que le ventilateur (du moteur sans brosses) fonctionne correctement. Quand vous installez l'extracteur, assurez-vous qu'ilyaunespace vide de 15 centimètres au moins entre le mur et l'arrière de l'extracteur pour assurer ventilation adéquate.

Le système SFE et les pompes de série 'D' utilisent les moteurs de courant continu. Ils possédent des collecteurs et brosses qui produisent des décharges électriques (étincelles) entre eux quand les moteurs fonctionnent normalement. Ces décharges, aussi bien que celles qui sont produites aux contacts des relais pourraient faire exploser un mixture d'air et de gaz inflammable. De petites modifications aux pompes peuvent les rendre moins dangereuses, surtout dans le cas rare d’une panne catastrophique du joint d’étanchéité du piston. Cependant, il n’y a aucune modification

qui fera ces pompes à l’épreuve d’explosion!

• Enlevez les couvercles de devant et d'arrière du cylindre qui sont situés sur la tour de la pompe. Cela permettra au gaz qui s'échappe de dissiper plus rapidement loin de la pompe et réduira aussi la quantité de gaz qui entre dans le compartiment du moteur.

• Scellez le compartiment du moteur de la pompe avec un ruban adhésif et circulez un flot continu d'azote (N2) à l’intérieur du compartiment. Cela réduira aussi la possibilité d'accumuler une mixture explosive autour du moteur et relais, où se trouvent la première possibilité des étincelles électriques.

• Pour l'information supplémentaire, téléphonez gratuitement (800) 775-2965, ou télécopiez (402) 465-3085 pour consulter le département de service technique.

UTILISEZ LE SYSTÈME SFE ET LES POMPES À SERINGUE TELEDYNE ISCO DANS TELLES

APPLICATIONS POTENTIELLEMENT HASARDEUSES Á VOS RISQUES ET PÉRILS!

D Series Syringe Pumps

Warnings, Cautions, and Notices

WARNING:

PLEASE READ

At the request of our Supercritical Fluid Extraction laboratory staff, we want our customers to be aware of the potential hazards involved with supercritical fluid extraction. Oxidizing gases, such as nitrous oxide, in contact with organic matrices or flammable modifiers, can detonate under certain conditions. Likewise, flammable fluids, such as methane, under high pressure conditions can present a hazard.

With concern for the safety of our customers, we have designed our extractors to be as safe as possible. However, we do not recommend the use of our instrument with potentially explosive reactions.

The letter below, which appeared in the July 22, 1991 edition of Chemical and Engineering News, is reprinted with permission from Professor Robert E. Sievers and his colleagues at the University of Colorado at Boulder. Even though they were not performing supercritical fluid extraction, it details the problems their lab experienced using nitrous oxide under similar conditions. We add our support for their suggestion to use only carbon dioxide or other less hazardous fluids for supercritical fluid extraction.

vii

D Series Syringe Pumps Warnings, Cautions, and Notices

CAUTION: Avoid spills! Liquids associated with this instrument may be classified as carcinogenic, biohazardous, flammable, or radioactive. Should these liquids be used, it is highly recommended that this application be accomplished in an isolated environment designed for these types of materials in accordance with federal state and local regulatory laws and in compliance with your organization’s chemical/hygiene plan in the event of a spill. In all cases, when using Teledyne Isco instrumentation, prudence and common sense must be used.

WARNING:

Pinch point. This symbol warns you that your fingers or hands will sustain serious injury if you place them between the moving parts of the mechanism near this symbol.

WARNING:

Avoid hazardous practices! If you use this instrument in any way not specified in this manual, the protection provided by the instrument may be impaired; this will increase your risk of injury.

CAUTION: Liquids associated with this instrument may be classified as carcinogenic, biohazardous, flammable, or radioactive. Should these liquids be used, it is highly recommended that this application be accomplished in an isolated environment designed for these types of materials, in accordance with federal, state, and local regulatory laws, and in compliance with your company’s chemical/hygiene plan in the event of a spill. In all cases, when using Teledyne Isco instrumentation, prudence and common sense must be used.

WARNING: Team lift.To reduce the risk of injury do not attempt to lift this

instrument independently. It is highly recommended that a team be used when lifting this instrument.

viii

D Series Syringe Pumps

Warnings, Cautions, and Notices

AVIS: Éviter de répandre! Les liquides qui sont pompés dans cet instrument peuvent être cancérigènes, hasards biologiques, inflammables, ou radioactifs. Si vous devez utiliser ces liquides hasardeux, il est très recommandé que vous le faites à l'intérieur d'un environnement isolé conçu pour tels liquides. Cet environnement isolé devrait être construit selon les règlements fédéraux, provinciaux, et locaux, aussi que le plan de votre organisation qui concerne l'évènement d'un accident avec les matières hasardeuses. En tout cas, utilisez toujours l'instrumentation d'Isco avec prudence et sens commun

ATTENTION: Risque de pincement. Ce symbole vous avertit que les mains ou les doigts recevront une blessure sérieuse si vous les mettez entre les éléments en mouvement du mécanisme près de ce symbole.

ATTENTION: Éviter les usages hasardeux! Si vous utilisez cet instrument d’une manière autre que celles qui sont specifiées dans ce manuel, la protection fournie par l’instrument peut être affaiblie; cela augmentera votre risque de blessure.

AVIS: Les liquides qui sont analysés dans cet instrument peuvent être cancérigènes, hasards biologiques, inflammables, ou radioactifs. Si vous devez utiliser ces liquides hasardeux, il est très recommandé que vous le faites à l'intérieur d'un environnement isolé conçu pour tels liquides. Cet environnement isolé devrait être construit selon les règlements fédéraux, provinciaux, et locaux, aussi que le plan de votre organisation qui concerne l'évènement d'un accident avec les matières hasardeuses. En tout cas, utilisez toujours l'instrumentation d'Isco avec prudence et sens commun.

D Series Syringe Pumps Warnings, Cautions, and Notices

Commonly Ordered Replacement Parts for the

Description Part Number

Cylinder Seals General:

30D Seal Assembly 0-2068 bar 60-1249-128 30D Piston Assembly 0-2068 bar 60-1249-129

65D 0-1379 bar 202-9096-08 65DM 0-689.5 bar 202-9096-08 100DX/DM 0-689.5 bar 202-9090-75 260D 0-517.1 bar 202-9091-06 500D 0-258.6 bar 202-9091-56 500HPx 0-344.7 bar 202-9091-56 1000D 0-137.8 bar upper seal 202-9990-25 1000D 0-137.8 bar lower seal 202-9990-23

(For more information on seals, refer to Table 2-7.)

65D/30D

Inlet/Outlet fittings:

4" F250C Gland 209-0164-02

4" F250C Collar 209-0164-03

4" F250C Plug 209-0164-05

65DM

Inlet/Outlet fittings:

Valco -18" Nut 209-0169-27

8" Ferrule 209-0169-41

8" Plug 209-0166-80

8 –116" Tubing Reducer 209-0169-42

100DX/DM and 260D

Inlet/Outlet fittings: Valco -18" Nut 209-0169-27

16" Nut 209-0094-07

8" Plug 209-0166-80

8 –116" Tubing Reducer 209-0169-42

500D

Inlet/Outlet fittings:

8" Tubing Connector to18" NPT 209-0161-01

8" NPT Plug 209-0168-00

8 –116" Tubing Reducer 209-0162-00

500 HPx

Inlet/outlet fittings:

8" Tubing Connector to

8" NPT Plug 209-016-813

D Series Syringe Pumps

8" NPT 209-016-100

D Series Syringe Pumps

Warnings, Cautions, and Notices

Commonly Ordered Replacement Parts for the

D Series Syringe Pumps

Description Part Number

1000D

Inlet/Outlet fittings:

4" NPT Plug 209-0168-09

4" NPT Connector to14" tubing 209-1063-36

Stainless Steel Tubing:

16"OD0.020" ID 004-7300-21

8"OD0.069" ID 004-7302-22

D Series Syringe Pumps Warnings, Cautions, and Notices

-NOTICE-

If you have ordered one of the following custom pumps from Teledyne

Isco, technical specifications for tubing and fittings listed in this manual

do not apply to your system.

Contact the factory for details about your custom pump.

Models:

65HP 260HP 500HP 500HV

500SP

xii

D Series Syringe Pumps

Section 1 Introduction

1.1 About This Manual ..................................................1-1

1.2 Specifications .......................................................1-1

1.3 Unpacking ........................................................1-13

1.4 Controls and Indicators..............................................1-14

1.5 Electrical Connections...............................................1-18

1.5.1 Cabling .....................................................1-19

1.6 Preliminary Checkout...............................................1-21

Section 2 Fluid System Connections and Accessories

2.1 Introduction ........................................................2-1

2.2 Fluid System Connections ............................................2-1

2.2.1 Ports ........................................................ 2-1

2.2.2 Installation Tips ...............................................2-2

2.2.3 Tubing Requirements ..........................................2-2

2.2.4 Accessory Packages ............................................2-3

2.2.5 Installing the

2.2.6 Draining Overflow .............................................2-5

2.2.7 Cylinder Washing: Wash Gland ..................................2-5

2.2.8 Cylinder Washing: Nitrogen Purge ................................2-7

2.2.9 Flushing Pumps with External Transducers ........................2-8

2.3 Fluid Connection Accessories ..........................................2-8

2.3.1 Manual Refill Kit ..............................................2-8

2.3.2 Manual Outlet Valve Kit ........................................2-9

2.3.3 CO

2.3.4 In-Line Filter Package .........................................2-11

2.4 Temperature and Pressure Controls ...................................2-12

2.4.1 Cylinder Insulating Cover ......................................2-12

2.4.2 Temperature Control Jacket ....................................2-13

2.4.3 Back Pressure Regulation ......................................2-18

2.5 Seal Options.......................................................2-19

Cylinder Connection Package ...............................2-10

/16" Fittings (260D, 65DM, and 100DM/DX only) ........2-4

Section 3 Basic Programming and Operation

3.1 Introduction ........................................................3-1

3.2 General Controller Information ........................................3-1

3.2.1 Rates, Units, and Limits ........................................3-1

3.2.2 Programming Screens ..........................................3-1

3.2.3 Run Screens ..................................................3-2

3.2.4 Selecting Operating Parameters ..................................3-3

3.3 Main Menus........................................................ 3-3

3.4 Menu One..........................................................3-3

3.4.1 Flow and/or Pressure Units of Measure ............................3-3

3.4.2 Pump Selection

(if multiple pumps) ............................................3-4

3.4.3 Refill ........................................................3-4

3.4.4 Power Failure [STOP] ..........................................3-5

xiii

D Series Syringe Pumps Table of Contents

3.5 Menu Two .........................................................3-5

3.6 Menu Three ........................................................3-8

3.7 Menu Four .........................................................3-9

3.8 Front Panel Keys...................................................3-10

3.9 Control of Multiple Pumps ...........................................3-13

3.10 Operating Modes ..................................................3-15

3.11 External Control ..................................................3-18

3.12 External Control: Analog ...........................................3-18

3.13 Remote RUN/STOP................................................3-21

3.14 Analog Flow Rate and Volume Output Options .........................3-22

3.4.5 System Reset (Restore Default Settings) ...........................3-5

3.4.6 Display Contrast ..............................................3-5

3.5.1 Serial Option .................................................3-6

3.5.2 Pump Status ..................................................3-6

3.5.3 External Control ..............................................3-6

3.5.4 Multiple Pumps ...............................................3-6

3.5.5 Total Volume Reset ............................................3-6

3.5.6 Valve ........................................................3-7

3.6.1 Poor Fill Alarm ................................................3-8

3.6.2 Diagnostic Menu ..............................................3-8

3.6.3 Pressure Calibration ...........................................3-8

3.6.4 Diff. Modes ...................................................3-9

3.6.5 Press. Integral ................................................3-9

3.6.6 Pressure Deadband ............................................3-9

3.7.1 Modbus Options ...............................................3-9

3.8.1 CONST PRESS and CONST FLOW .............................. 3-10

3.8.2 PRGM GRAD ................................................3-10

3.8.3 HOLD ......................................................3-10

3.8.4 RECALL ....................................................3-10

3.8.5 STORE .....................................................3-10

3.8.6 REFILL .....................................................3-10

3.8.7 LIMITS .....................................................3-10

3.8.8 RAPID PRESS ...............................................3-12

3.8.9 DISP .......................................................3-13

3.8.10 ACC CTRL .................................................3-13

3.8.11 ZERO PRESS ...............................................3-13

3.9.1 Multi-Pump Operation ........................................ 3-14

3.9.2 Independent Control of up to Four Separate Pumps .................3-14

3.10.1 Constant Flow ..............................................3-16

3.10.2 Constant Pressure ...........................................3-16

3.10.3 Dispense Mode ..............................................3-17

3.12.1 Wire connections ............................................

3.12.2 Preparation ................................................3-19

3.12.3 External Control for Refill .....................................3-19

3.12.4 Calculation Examples ........................................3-20

3.12.5 Setup ......................................................3-20

3.13.1 Wire Connections ............................................3-21

3.14.1 Voltage Analog Output .......................................3-22

3.14.2 Current Loop Output .........................................3-23

3-18

xiv

Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes

4.1 Introduction ........................................................4-1

4.2 Connecting the System ...............................................4-2

4.2.1 Inlet Connections ..............................................4-4

4.2.2 Outlet Connections ............................................ 4-5

4.2.3 Dual Gradient System Connections ...............................4-6

D Series Syringe Pumps

Table of Contents

4.3 Single-Pump Gradient Programming ...................................4-8

4.4 Dual Pump Concentration Gradient Programming .......................4-10

4.5 Review, Revise, & Hold Options .......................................4-12

4.6 Program Conclusion ................................................4-12

Section 5 Continuous Flow Introduction, Installation, & Operation

5.1 Introduction ........................................................5-1

5.2 Continuous Flow Check Valves ........................................5-1

5.2.1 Check Valve Technical Specifications ..............................5-2

5.2.2 Dual Check Valve Installation ...................................5-2

5.2.3 Inlet Tubing ..................................................5-3

5.3 Continuous Flow Air Valves ...........................................5-7

5.3.1 Dual Air Valve Installation ......................................5-7

5.4 Continuous Flow Electric Valves ......................................5-13

5.4.1 Technical Specifications .......................................5-13

5.4.2 Dual Electric Valve Installation .................................5-14

5.5 User Supplied Valves ...............................................5-16

5.6 Continuous Flow Mode ..............................................5-17

5.6.1 Defining Operation ...........................................5-17

5.6.2 Constant Flow Mode ..........................................5-18

5.6.3 Constant Pressure Mode .......................................5-19

5.6.4 No Check Valve ..............................................5-20

5.6.5 To Run or Stop ...............................................5-21

5.6.6 Tips for Running Continuous Flow ...............................5-21

Section 6 Modifier Addition

6.1 Overview ..........................................................6-1

6.1.1 Installing the Kit ..............................................6-1

6.1.2 Modifier Mode Setup ...........................................6-4

6.1.3 Two Pump Operation ...........................................6-5

6.2 Programming .......................................................6-6

6.2.1 Mininum Modifier Pressure Setting ...............................6-7

6.2.2 Modifier Concentration .........................................6-7

6.3 Refilling ...........................................................6-8

Section 7 Modbus Configuration

7.1 Overview ..........................................................7-1

7.1.1 Modbus RTU .................................................7-1

7.1.2 Modbus TCP/IP ...............................................7-2

7.1.3 Modbus Configuration Options ...................................7-2

Section 8 Serial Interface

8.1 Overview ..........................................................8-1

8.2 Network Control and Communication ...................................8-2

8.3 USB Interface ......................................................8-3

8.4 Cabling for Serial Control .............................................8-3

8.4.1 One Controller ................................................8-3

8.4.2 Two Controllers (Compiler required) ..............................8-4

8.4.3 Three or More Controllers (Compiler required) ......................8-4

8.5 Serial Control Check List ............................................. 8-5

8.6 Controller Setup ....................................................8-5

8.6.1 Restore Defaults (if desired) .....................................8-5

8.6.2 Change Defaults ...............................................8-5

8.6.3 Verify Operation ..............................................8-6

D Series Syringe Pumps Table of Contents

8.7 User-written Software................................................8-6

8.8 Serial Commands for the D Series Pump ...............................8-20

Section 9 Pump Maintenance, Troubleshooting, and Servicing

9.1 Introduction ........................................................9-1

9.2 Maintenance Kits ...................................................9-3

9.3 General Cleaning....................................................9-4

9.4 Resetting the System ................................................9-4

9.5 Diagnostic Menu ....................................................9-6

9.6 Removing the Case Top...............................................9-9

9.7 Test Points........................................................9-11

9.8 Lubrication .......................................................9-12

9.9 Seal Cleaning and Replacement .......................................9-13

9.10 Wear Ring Cleaning and Replacement ................................9-17

9.11 Flushing the Cylinder ..............................................9-17

9.12 Overpressure Conditions ...........................................9-18

9.13 Motor Brushes ....................................................9-23

9.14 Calibrations ......................................................9-35

8.7.1 DASNET .....................................................8-6

8.7.2 Universal Driver from Teledyne Isco ..............................8-7

8.8.1 Get Status Command .........................................8-25

8.8.2 Gradient Download Command ..................................8-29

8.8.3 Gradient Upload Commands ....................................8-29

8.8.4 Range Command .............................................8-33

8.8.5 Error Messages .............................................. 8-34

9.1.1 Service Department ............................................9-2

9.1.2 How to Ship Returns ...........................................9-2

9.4.1 Basic Reset ...................................................9-4

9.4.2 Hard Reset ...................................................9-4

9.5.1 LCD TEST ................................................... 9-6

9.5.2 ANALOG INPUTS .............................................9-6

9.5.3 KEYPAD TEST ...............................................9-7

9.5.4 MOTOR CONTROL/LIMITS .....................................9-7

9.5.5 SERIAL TEST ................................................9-8

9.6.1 Controller Case Top Removal ....................................9-9

9.6.2 Pump Case Top Removal .......................................9-10

9.7.1 Power Supply (A1) ............................................9-11

9.7.2 Controller ...................................................9-11

9.8.1 Ball Nut ....................................................9-12

9.9.1 Piston Seal

(Excludes 30D) ...............................................9-14

9.9.2 Piston Seal Break-In (Aqueous seals only) .........................9-14

9.9.3 All other piston seals ..........................................9-15

9.9.4 Wiper Seal

(Excludes 30D) ...............................................9-15

9.11.1 Gas Solvent Changeover ......................................9-17

9.11.2 Liquid Solvent Changeover and Flushing ........................9-18

9.12.1 Shear Key ..................................................9-19

9.12.2 Replacement Using Installation Tool ............................ 9-20

9.12.3 Replacement Without Installation Tool ..........................9-21

9.12.4 Pump Bearings

(30D and 65DM Only) .........................................9-21

9.12.5 Completion of Shear Key Replacement ..........................9-22

9.13.1 Motor Brush Disassembly .....................................9-25

9.13.2 HPx Motor Brush Replacement ................................ 9-26

9.14.1 Pressure Transducer Calibration ...............................9-35

9.14.2 A/D Circuit Adjustment .......................................9-35

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Table of Contents

9.14.3 Limit Sensor Adjustment .....................................9-35

9.14.4 Limit Sensor Calibration

(Excludes 30D) ...............................................9-39

9.14.5 30D Limit Sensor Calibration ..................................9-40

9.14.6 Electric Valve Motor Calibration ...............................9-41

Appendix A Replacement Parts

A.1 Replacement Parts ..................................................A-1

A.1.1 D Series Controller ............................................A-2

A.1.2 1000D Pump .................................................A-5

A.1.3 500D/DX Pump ...............................................A-9

A.1.4 500HPx Pump ...............................................A-13

A.1.5 100DM/DX and 260D Pumps ...................................A-16

A.1.6 65D Pump ..................................................A-20

A.1.7 65DM ......................................................A-33

A.1.8 Power Circuit Board ..........................................A-47

A.1.9 Analog Output Circuit Board ...................................A-51

A.1.10 Interface Circuit Board .......................................A-53

A.1.11 Dual Electric Valve Package ...................................A-55

A.1.12 Single Electric Valve Package .................................A-57

A.1.13 SST Dual Air Valve Package for 100/260D/500D ..................A-59

A.1.14 SST Dual Air Valve Package for 1000D ..........................A-61

A.1.15 Single Air Valve Package .....................................A-63

A.1.16 Dual Air Valve Package for 500HPx ............................A-65

A.1.17 Dual Check Valve Package for 100/260/500D .....................A-67

A.1.18 Dual Check Valve Package for 1000D ...........................A-69

A.1.19 Single Check Valve Package ...................................A-71

Appendix B Continuous Flow Introduction, Installation, and Operation for 30D

B.1 Introduction .......................................................B-1

B.2 Continuous Flow Air Valves...........................................B-1

B.2.1 Dual Air Valve Installation .....................................B-2

B.3 Continuous Flow Electric Valves .......................................B-4

B.3.1 Technical Specifications ........................................B-4

B.3.2 Dual Electric Valve Installation ..................................B-5

B.4 User Supplied Valves ................................................B-7

B.5 Continuous Flow Mode ...............................................B-8

B.5.1 Defining Operation ............................................B-9

B.5.2 Constant Flow Mode ..........................................B-10

B.5.3 Constant Pressure Mode .......................................B-10

B.5.4 No Check Valve ..............................................B-11

B.5.5 To Run or Stop ...............................................B-12

B.5.6 Tips for Running Continuous Flow ..............................B-12

B.6 Replacement Parts List .............................................B-14

B.6.1 30D Pump ..................................................B-14

B.6.2 30D Air Valve ...............................................B-17

B.6.3 30D Electric Air Valve ........................................B-19

List of Figures

1-1 260D flow rate range ...............................................1-11

1-2 500D flow rate range ...............................................1-11

1-3 500 HPx flow rate range ............................................1-12

1-4 1000D flow rate range ..............................................1-12

xvii

D Series Syringe Pumps Table of Contents

1-5 Pump controller key functions ........................................1-14

1-6 Pump controller rear panel connectors .................................1-16

1-7 Pump rear panel connectors .........................................1-17

1-8 Pump front panel controls ...........................................1-18

1-9 Syringe pump cylinder cap: Attach to pressure transducer connector on back of

1-10 Connecting the pump and controller ..................................1-21

1-11 Status Screen ....................................................1-21

1-12 Menu 1 .........................................................1-22

1-13 Three pump stop menu ............................................1-23

1-14 Four pump stop menu .............................................1-23

2-1 Liquid system plumbing connections (30D on Left) ........................2-2

2-2

2-3 30D/65DFitting .....................................................2-4

2-4 Drain tube installation ...............................................2-5

2-5 Drain tube installation and wash gland connection ........................2-6

2-6 Purge connector installation ..........................................2-7

2-7 Refill kit installation ................................................2-9

2-8 Outlet valve package connection ......................................2-10

2-9 CO

2-10 In-line filter package ..............................................2-11

2-11 Cylinder insulation cover ...........................................2-12

2-12 System with temperature control jacket installed .......................2-13

2-13 Fluid fittings .....................................................2-14

2-14 Secure screws with the

2-15 Removing the cylinder cap with wrenches .............................2-16

2-16 Temperature control jacket installation ...............................2-17

2-17 Back pressure regulator ............................................2-18

2-18 Inside the pump cylinder: wetted materials ............................2-19

3-1 Menu 1 program selections ...........................................3-3

3-2 Units menu ........................................................3-3

3-3 Menu 2 program selections ...........................................3-5

3-4 Serial option menu ..................................................3-6

3-5 Total volume reset ..................................................3-6

3-6 Types of valves .....................................................3-7

3-7 Menu 3 program selections ...........................................3-8

3-8 Location of transducer midpoint value ..................................3-8

3-9 Limits menu ......................................................3-11

3-10 Limits setpoint (Max Press) menu ...................................3-11

3-11 Depiction of dispense mode sequence .................................3-17

3-12 External control for refill menu ......................................3-20

3-13 Button sequence for external control setup ............................3-21

3-14 Analog voltage output connection, rear panel ..........................3-22

3-15 Output range selection jumpers ..................................... 3-23

4-1 Pump inlet connections ..............................................4-4

4-2 Pump outlet connections .............................................4-5

4-3 Dual gradient system connections

4-4 Dual gradient connections: Detail ......................................4-7

5-1 Check valve package installation: 65DM/100D/265D/500D pumps ...........5-4

5-2 Check valve package installation: 1000D pump ...........................5-6

5-3 Air valve installation for 65DM, 100DM/DX, 260D, and 500D pumps ......... 5-9

5-4 Air valve installation for 500HPx pumps ...............................5-10

5-5 Air valve installation for 1000D pump .................................5-11

5-6 Air valve installation for 65D pump ...................................5-12

5-7 Electric valve installation ...........................................5-15

5-8 Keystrokes to specify valve type ......................................5-18

5-9 Keystrokes to reset volume totalizer ...................................5-18

5-10 Keystrokes to set up constant flow ...................................5-19

pump. ...........................................................1-20

/16" Tubing adapter for 30D/65D pumps ................................ 2-4

package installation ............................................2-11

/4” hex wrench ...............................2-15

(Inlet valves (A & C) are from refill valve kits) ...........................4-7

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D Series Syringe Pumps

Table of Contents

5-11 Keystrokes to set up constant pressure ...............................5-19

5-12 Keystrokes to set up No Check Valve .................................5-20

5-13 Keystrokes to Run or Stop the pumps .................................5-21

6-1 Modifier addition kit installation (Two-pump configuration) ................ 6-2

6-2 Modifier addition kit installation (continuous flow) ........................6-3

6-3 Minimum modifier pressure screen .....................................6-7

7-1 RS-485 Connection ..................................................7-1

8-1 Serial network connection example - Single connection .................... 8-4

8-2 Serial network connection example - Dual connection ......................8-4

8-3 Get Status String “G” Command ......................................8-26

8-4 Get Status String “GG” Command ....................................8-26

8-5 Get All Status String “G&” Command .................................8-27

8-6 Get all status strings from four pump operation “G&2” Command ..........8-28

8-7 Gradient Download Commands - Single pump flow gradient ...............8-29

8-8 Gradient Download Commands - Two pump flow gradient .................8-30

8-9 Gradient Upload Commands - Single pump flow gradient .................8-31

8-10 Gradient Upload Commands - Two pump flow gradient ..................8-32

8-11 Upload Commands - Single pump pressure programming ................8-33

8-12 Range Serial Commands ...........................................8-33

9-1 500HPx fan filter ...................................................9-4

9-2 Accessing the diagnostic menu ........................................9-6

9-3 Analog Input diagnostic screen ........................................9-6

9-4 Optical sensor, interrupted by flag (bottom sensor shown, rear tower cover plate re-

moved) ........................................................... 9-7

9-5 Serial connection to a computer .......................................9-8

9-6 Controller case top screws (2 of 4 shown) ...............................9-10

9-7 Pump case top screws (2 of 4 shown) ...................................9-10

9-8 Gear train lubrication and motor drive service ..........................9-12

9-9 Break-in procedure for aqueous seals (Drawing does not apply to the 30D) . . . 9-15

9-10 Accessing the shear key ............................................9-20

9-11 Shear key replacement .............................................9-20

9-12 30D and 65DM Bearing set ......................................... 9-22

9-13 30D and 65DM Shear key replacement ...............................9-22

9-14 D Series motor brush replacement ...................................9-24

9-15 Location of motor brushes ..........................................9-24

9-16 Length of a new motor brush (1.1 cm) .................................9-24

9-17 Inserting new motor brush .........................................9-25

9-18 For 30D a cable tie holds the wires together ...........................9-25

9-19 Remove screws and washers, then the plate ...........................9-26

9-20 Remove the five screws from the bottom of the pump .................... 9-26

9-21 two pin connector .................................................9-27

9-22 Encoder .........................................................9-27

9-23 Location of screws and washers holding the motor in place ...............9-28

9-24 Motor assembly ...................................................9-28

9-25 Support plate ....................................................9-29

9-26 Position of wires connected to the motors ..............................9-29

9-27 Wire connections ..................................................9-29

9-28 Set screws holding the two negative brushes ...........................9-30

9-29 Wires disconnected from brush contact ...............................9-30

9-30 Connector connected to the brush ....................................9-31

9-31 Removing the set screws ...........................................9-31

9-32 Loosen the connector ..............................................9-32

9-33 New brush reinstalled; set screw replaced .............................9-32

9-34 Location of locating pins ...........................................9-33

9-35 Motor plate and bearing plate .......................................9-34

9-36 Installing the deflector shield .......................................9-34

9-37 Capacitor and motor shaft ..........................................9-35

9-38 Limit sensor replacement ..........................................9-37

9-39 Installing the plastic mounts ........................................9-37

xix

D Series Syringe Pumps Table of Contents

9-40 Electric valve motor voltage ......................................... 9-42

9-41 Electric valve motor adjustment .....................................9-43

B-1 Air valve installation for 30D pump ....................................B-3

B-2 Electric valve installation ............................................B-6

B-3 Keystrokes to specify valve type .......................................B-9

B-4 Keystrokes to reset volume totalizer ...................................B-9

B-5 Keystrokes to set up constant flow ....................................B-10

B-6 Keystrokes to set up constant pressure ................................B-10

B-7 Keystrokes to set up No Check Valve ..................................B-11

B-8 Keystrokes to Run or Stop the pumps .................................B-12

List of Tables

1-1 30D Technical Specifications ..........................................1-2

1-2 65D Technical Specifications ..........................................1-3

1-3 65DM Technical Specifications ........................................1-4

1-4 100DM Technical Specifications .......................................1-5

1-5 100DX Technical Specifications ........................................1-6

1-6 260D Technical Specifications .........................................1-7

1-7 500D Technical Specifications .........................................1-8

1-8 500HPx Technical Specifications .......................................1-9

1-9 1000D Technical Specifications .......................................1-10

1-10 Pump Controller Front Panel Label ..................................1-14

1-11 Pump Controller Key Functions .....................................1-15

1-12 Pump Controller Rear Panel Connectors ..............................1-16

1-13 Pump Rear Panel Connectors .......................................1-17

1-14 Pump Front Panel ................................................1-18

2-1 D Series Syringe Pump Accessory Packages .............................2-3

2-2 Manual Refill Kits ..................................................2-9

2-3 Manual Outlet Valve Kits ...........................................2-10

2-4 CO

2-5 Optional in-line Filter Package 68-1247-011 ............................ 2-11

2-6 Packages and Parts ................................................2-14

2-7 Seal Selection Chart ................................................2-20

3-1 Key functions in the Multi-pump Mode ................................3-14

3-2 Analog Output Options (connections to female 25 pin Sub-D) ..............3-23

3-3 Analog Output Signal/Sub-D Pins for 4-20mA ...........................3-24

3-4 Analog Output Signal/Sub-D Pins for 0-10 VDC .........................3-25

4-1 Manual Refill Valve Kits .............................................4-1

4-2 Manual Outlet Valve Kits ............................................4-2

4-3 Swaging Detail .....................................................4-2

5-1 Continuous Flow Technical Specifications-Check Valves: ...................5-2

5-2 Continuous Flow Check Valve Package for

5-3 Continuous Flow Check Valve Package for 1000D, part #68-1247-128 ........5-6

5-4 Continuous Flow Technical Specifications-Air Valves: .....................5-7

5-5 Continuous Flow Technical Specifications-Electric Valves: ................5-13

5-6 Accessory Control Digital Outputs ....................................5-16

5-7 Key functions in the Multi-pump Mode ................................5-17

6-1 Modifier Addition Kit for Two Pumps (part #68-1247-079) ..................6-3

6-2 Modifier Addition Components for Continuous Flow System ................6-4

7-1 Modbus TCP/IP Configuration Options .................................7-2

7-2 Modbus RTU Configuration Options ....................................7-2

7-3 Supported Modbus Function Codes .....................................7-3

7-4 Exception Responses ................................................7-3

7-5 Coils ..............................................................7-4

Cylinder Connection Package 68-1247-043 ..........................2-10

100D/260D /500D, part #68-1247-059 ................................... 5-5

D Series Syringe Pumps

Table of Contents

7-6 Holding Registers ...................................................7-7

8-1 External control connector

serial pin connections ...............................................8-2

8-2 USB Interface Pin Connections ........................................8-3

8-3 Example of BASIC program to demonstrate

conversion of pump commands to DASNET frames .......................8-8

8-4 Example of C program to demonstrate

conversion of pump commands to DASNET frames .......................8-9

8-5 Example of a Visual C++ program to demonstrate conversion

of pump commands to DASNET frames ................................ 8-10

8-6 Serial Commands ..................................................8-20

8-7 Error Messages ....................................................8-34

9-1 Maintenance Kit Contents ............................................9-3

9-2 Maintenance Kit Part Numbers .......................................9-4

9-3 Piston Wrench Tools ................................................9-16

9-4 Replacement Shear Keys ............................................9-19

B-1 Continuous Flow Technical Specifications-Air Valves: .....................B-2

B-2 Continuous Flow Technical Specifications-Electric Valves: .................B-4

B-3 Accessory Control Digital Outputs .....................................B-7

B-4 Key functions in the Multi-pump Mode .................................B-8

xxi

D Series Syringe Pumps Table of Contents

xxii

D Series Syringe Pumps

Section 1 Introduction

1.1 About This Manual The 65DM, 100DM, 100DX, 260D, and 500D pumps have

standard Valco ports. Pipe thread fittings of14" diameter are used for the 1000D,38" diameter for the 500HPx, and18" diameter for the 500D. The 65D and 30D use AE F250C high pressure fittings. Because of these differences, the packages, tubing, and options for different models have different part numbers. Additionally, these pumps are typically used for different applications; therefore, optional kits and accessories may differ.

The installation procedures for the different D Series pump models have been combined where applicable, and separated where necessary, for your convenience.

Optional system configurations may require additional kits and/or software. These additional options are described in their respective sections.

This manual contains the following sections: Section 1 Introduction Section 2 Fluid System Connections and Accessories Section 3 Basic Programming and Operation Section 4 Gradient Pumping for Pressure, Flow, and Concen-

tration Modes Section 5 Continuous Flow Introduction, Installation, & Oper-

ation

Section 6 Modifier Addition Section 7 Modbus Configuration Section 8 Serial Interface Section 9 Pump Maintenance, Troubleshooting, and Servicing Appendix A Replacement Parts

8"

1.2 Specifications The technical specifications for the D Series Syringe Pumps are

detailed in Tables 1-2 through 1-9.

Note

Underwriters Laboratories (UL) has certified all Series D Pumps with the exception of the 100 VAC versions.

1-1

D Series Syringe Pumps Section 1 Introduction

Table 1-1 30D Technical Specifications

CYLINDER CAPACITY 30.42 ml

POWER REQUIREMENTS

(Mains voltage line cord is a “Disconnect Device”)

LINE FREQUENCY 50 or 60 Hz

LINE VOLTAGE NOISE TOLERANCE 1.7

DIMENSIONS PUMP CONTROLLER

WEIGHT PUMP CONTROLLER

FLOW RATE RANGE 0.01 µl/min to 22 ml/min (for any pressure up to 2068 bar)

FLOW RATE ACCURACY

FLOW RATE DISPLAY RESOLUTION 0.01 µl/min (1.0 µl/min in Constant Pressure Mode)

ANALOG OUTPUT ACCURACY

DISPLACEMENT RESOLUTION 1.825 nl /step

REFILL TIME 1.05 minutes

REFILL OR DEPRESSURIZATION RATE 0.01 µl/min to 29 ml/min at any pressure from 0 to 2068 bar

100 ± 10 VAC, 2.8 A maximum 117 ± 12 VAC, 2.4 A maximum 234 ± 23 VAC, 1.2 A maximum

Factory Set

}

 nominal rms line voltage, 10 µsecond pulses, any phase

angle, random or repetitive

Width: 27.18 cm 27.18 cm Depth: 46.74 cm 30.48 cm Height: 100 cm 13.59 cm

36.4 kg 2.96 kg

± 0.5% above 69 bar and above 0.25 ml/min (maximum 1.0 µl/min seal leakage)

± 0.2% 4-20 mA output, ± 0.5% voltage output using the 12 channel output option

PRESSURE RANGE 0.6895 to 2068.4 bar

PRESSURE ACCURACY ± 0.2% of full scale at constant temperature

PRESSURE REPEATABILITY

ZERO PRESSURE DRIFT ± 0.05% of full scale within 48 hours at constant temperature

PRESSURE DISPLAY RESOLUTION 6.895 kPa

AMBIENT TEMPERATURE RANGE 5 to 40°C

TEMPERATURE DRIFT ± 0.015% of full scale/°C

HUMIDITY 95% maximum

DEAD (HEADSPACE) VOLUME

POLLUTION DEGREE 2

INSTALLATION CATEGORY II

MAXIMUM ALTITUDE 2000 m

a. Using water at constant pressure and constant temperature above 69 bar and above 0.25 ml/min.

b. The analog output is an optional accessory.

c. Pressure repeatability specification is based on zeroing the pressure transducer before measurement. Refer to sub-section

ZERO PRESS in Section 3 of the manual for re-zeroing procedure.

d. Volume in and above the piston seal, head clearance at automatic shutoff, and inlet and outlet ports to the fittings.

± 0.05% of full scale within 48 hours at constant temperature

0.88 ± 0.02 ml

1-2

Table 1-2 65D Technical Specifications

CYLINDER CAPACITY 67.97 ml

POWER REQUIREMENTS

(Mains voltage line cord is a “Disconnect Device”)

LINE FREQUENCY 50 or 60 Hz

100 ± 10 VAC, 1.5 A maximum 117 ± 12 VAC, 1.5 A maximum 234 ± 23 VAC, 0.75 A maximum

Factory Set

}

D Series Syringe Pumps

Section 1 Introduction

LINE VOLTAGE NOISE TOLERANCE 1.7

DIMENSIONS PUMP CONTROLLER

WEIGHT PUMP CONTROLLER

FLOW RATE RANGE 0.01 µl/min to 25 ml/min (for any pressure up to 1379 bar)

FLOW RATE ACCURACY

FLOW RATE DISPLAY RESOLUTION 0.01 µl/min (1.0 µl/min in Constant Pressure Mode)

ANALOG OUTPUT ACCURACY

DISPLACEMENT RESOLUTION 2.55 nl /step

REFILL TIME 1.7 minutes

REFILL OR DEPRESSURIZATION RATE 0.01 µl/min to 40 ml/min at any pressure from 0 to 1379 bar

PRESSURE RANGE 0.6895 to 1379 bar

PRESSURE ACCURACY ± 0.1% of full scale at constant temperature

PRESSURE REPEATABILITY

ZERO PRESSURE DRIFT ± 0.25% of full scale within 48 hours at constant temperature

PRESSURE DISPLAY RESOLUTION 6.895 kPa

 nominal rms line voltage, 10 µsecond pulses, any phase

angle, random or repetitive

Width: 27.18 cm 27.18 cm Depth: 46.74 cm 30.48 cm Height: 101.09 cm 13.59 cm

32.8 kg 2.96 kg

± 0.3% (maximum 0.25 µl/min seal leakage)

± 1% of selected range

± 0.5% of full scale within 48 hours at constant temperature

AMBIENT TEMPERATURE RANGE 5 to 40°C

TEMPERATURE DRIFT ± 0.015% of full scale/°C

HUMIDITY 95% maximum

DEAD (HEADSPACE) VOLUME

POLLUTION DEGREE 2

INSTALLATION CATEGORY II

MAXIMUM ALTITUDE 2000 m

a. Underwriters Laboratories (UL) has certified all D Series Pumps with the exception of the 100 VAC versions.

b. Using water at 137.9 bar and a temperature controlled environment at 30°C.

c. The analog output is an optional accessory.

d. Pressure repeatability specification is based upon re-zeroing pressure transducer every 48 hours. Refer to sub-section

ZERO PRESS in Section 3 of the manual for re-zeroing procedure.

e. Volume in and above the piston seal, head clearance at automatic shutoff, and inlet and outlet ports to the fittings.

1.30 ± 0.020 ml

1-3

D Series Syringe Pumps Section 1 Introduction

Table 1-3 65DM Technical Specifications

CYLINDER CAPACITY 67.97 ml

POWER REQUIREMENTS

(Mains voltage line cord is a “Disconnect Device”)

LINE FREQUENCY 50 or 60 Hz

LINE VOLTAGE NOISE TOLERANCE 1.7

100 ± 10 VAC, 1.5 A maximum 117 ± 12 VAC, 1.5 A maximum 234 ± 23 VAC, 0.75 A maximum

 nominal rms line voltage, 10 µsecond pulses, any phase

angle, random or repetitive

Factory Set

}

DIMENSIONS PUMP CONTROLLER

WEIGHT PUMP CONTROLLER

FLOW RATE RANGE 0.01 µl/min to 30 ml/min (for any pressure up to 689 bar)

FLOW RATE ACCURACY

FLOW RATE DISPLAY RESOLUTION 0.01 µl/min (1.0 µl/min in Constant Pressure Mode)

ANALOG OUTPUT ACCURACY

DISPLACEMENT RESOLUTION 2.55 nl /step

REFILL TIME 1.7 minutes

REFILL OR DEPRESSURIZATION RATE 0.01 µl/min to 40 ml/min at any pressure from 0 to 689 bar

PRESSURE RANGE 0.6895 to 689 bar

PRESSURE ACCURACY ± 0.5% of full scale at constant temperature

PRESSURE REPEATABILITY

ZERO PRESSURE DRIFT ± 0.25% of full scale within 48 hours at constant temperature

PRESSURE DISPLAY RESOLUTION 6.895 kPa

AMBIENT TEMPERATURE RANGE 5 to 40°C

Width: 27.18 cm 27.18 cm Depth: 46.74 cm 30.48 cm Height: 103.0 cm 13.59 cm

33.2 kg 2.96 kg

± 0.3% of setpoint, (maximum 0.25 µl/min seal leakage)

± 1% of selected range

(± 0.1% FS optional)

± 0.5% of full scale within 48 hours at constant temperature

TEMPERATURE DRIFT ± 0.12% of full scale/°C

HUMIDITY 95% maximum

DEAD (HEADSPACE) VOLUME

POLLUTION DEGREE 2

INSTALLATION CATEGORY II

MAXIMUM ALTITUDE 2000 m

a. Underwriters Laboratories (UL) has certified all D Series Pumps with the exception of the 100 VAC versions.

b. Using water at 137.9 bar and a temperature controlled environment at 30°C.

c. The analog output is an optional accessory.

d. Pressure repeatability specification is based upon re-zeroing pressure transducer every 48 hours. Refer to sub-section

ZERO PRESS in Section 3 of the manual for re-zeroing procedure.

e. Volume in and above the piston seal, head clearance at automatic shutoff, and inlet and outlet ports to the fittings.

1.30 ± 0.020 ml

1-4

Table 1-4 100DM Technical Specifications

CYLINDER CAPACITY 102.93 ml

POWER REQUIREMENTS

(Mains voltage line cord is a “Disconnect Device”)

LINE FREQUENCY 50 or 60 Hz

100 ± 10 VAC, 1.5 A maximum 117 ± 12 VAC, 1.5 A maximum 234 ± 23 VAC, 0.75 A maximum

Factory Set

}

D Series Syringe Pumps

Section 1 Introduction

LINE VOLTAGE NOISE TOLERANCE 1.7

DIMENSIONS PUMP CONTROLLER

WEIGHT PUMP CONTROLLER

FLOW RATE RANGE 0.01 µl/min to 25 ml/min (for any pressure up to 689.5 bar)

FLOW RATE ACCURACY

FLOW RATE DISPLAY RESOLUTION 0.01 µl/min (1.0 µl/min in Constant Pressure Mode)

ANALOG OUTPUT ACCURACY

DISPLACEMENT RESOLUTION 4.825 nl /step

REFILL TIME 3.5 minutes

REFILL OR DEPRESSURIZATION RATE 0.01 µl/min to 30 ml/min at any pressure from 0 to 689.5 bar

PRESSURE RANGE 0.6895 to 689.5 bar

PRESSURE ACCURACY ± 0.5% of full scale at constant temperature

PRESSURE REPEATABILITY

ZERO PRESSURE DRIFT ± 0.25% of full scale within 48 hours at constant temperature

PRESSURE DISPLAY RESOLUTION 6.895 kPa

 nominal rms line voltage, 10 µsecond pulses, any phase

angle, random or repetitive

Width: 27.18 cm 27.18 cm Depth: 46.74 cm 30.48 cm Height: 101.09 cm 13.59 cm

32.8 kg 2.96 kg

± 0.3% (maximum 0.25 µl/min seal leakage)

± 1% of selected range

± 0.5% of full scale within 48 hours at constant temperature

AMBIENT TEMPERATURE RANGE 5 to 40°C

TEMPERATURE DRIFT ± 0.0.12% of full scale/°C

HUMIDITY 95% maximum

DEAD (HEADSPACE) VOLUME

POLLUTION DEGREE 2

INSTALLATION CATEGORY II

MAXIMUM ALTITUDE 2000 m

NOTES:

a. Underwriters Laboratories (UL) has certified all D Series Pumps with the exception of the 100 VAC versions.

b. Using water at 137.9 bar and a temperature controlled environment at 30°C.

c. The analog output is an optional accessory.

d. Pressure repeatability specification is based upon re-zeroing pressure transducer every 48 hours. Refer to sub-section

ZERO PRESS in Section 3 of the manual for re-zeroing procedure.

e. Volume in and above the piston seal, head clearance at automatic shutoff, and inlet and outlet ports to the fittings.

1.30 ± 0.020 ml

1-5

D Series Syringe Pumps Section 1 Introduction

Table 1-5 100DX Technical Specifications

CYLINDER CAPACITY 102.93 ml

POWER REQUIREMENTS

(Mains voltage line cord is a “Disconnect Device”)

LINE FREQUENCY 50 or 60 Hz

100 ± 10 VAC, 1.5 A maximum 117 ± 12 VAC, 1.5 A maximum 234 ± 23 VAC, 0.75 A maximum

Factory Set

}

LINE VOLTAGE NOISE TOLERANCE 1.7

DIMENSIONS PUMP CONTROLLER

WEIGHT PUMP CONTROLLER

FLOW RATE RANGE 0.01 µl/min to 50 ml/min (for any pressure up to 689.5 bar)

FLOW RATE ACCURACY

FLOW RATE DISPLAY RESOLUTION 0.01 µl/min (1.0 µl/min in Constant Pressure Mode)

ANALOG OUTPUT ACCURACY

DISPLACEMENT RESOLUTION 9.65 nl /step

REFILL TIME 1.72 minutes

REFILL OR DEPRESSURIZATION RATE 0.01 µl/min to 60 ml/min at any pressure from 0 to 689.5 bar

PRESSURE RANGE 0.6895 to 689.5 bar

PRESSURE ACCURACY ± 0.5% of full scale at constant temperature

PRESSURE REPEATABILITY

ZERO PRESSURE DRIFT ± 0.25% of full scale within 48 hours at constant temperature

PRESSURE DISPLAY RESOLUTION 6.895 kPa

 nominal rms line voltage, 10 µsecond pulses, any phase

angle, random or repetitive

Width: 27.18 cm 27.18 cm Depth: 46.74 cm 30.48 cm Height: 101.09 cm 13.59 cm

32.8 kg 2.96 kg

± 0.3% (maximum 0.25 µl/min seal leakage)

± 1% of selected range

± 0.5% of full scale within 48 hours at constant temperature

AMBIENT TEMPERATURE RANGE 5 to 40°C

TEMPERATURE DRIFT ± 0.0.12% of full scale/°C

HUMIDITY 95% maximum

DEAD (HEADSPACE) VOLUME

POLLUTION DEGREE 2

INSTALLATION CATEGORY II

MAXIMUM ALTITUDE 2000 m

a. Underwriters Laboratories (UL) has certified all D Series Pumps with the exception of the 100 VAC versions.

b. Using water at 137.9 bar and a temperature controlled environment at 30°C.

c. The analog output is an optional accessory.

d. Pressure repeatability specification is based upon re-zeroing pressure transducer every 48 hours. Refer to sub-section

ZERO PRESS in Section 3 of the manual for re-zeroing procedure.

e. Volume in and above the piston seal, head clearance at automatic shutoff, and inlet and outlet ports to the fittings.

1.30 ± 0.020 ml

1-6

Table 1-6 260D Technical Specifications

CYLINDER CAPACITY 266.05 ml

POWER REQUIREMENTS

(Mains voltage line cord is a “Disconnect Device”)

LINE FREQUENCY 50 or 60 Hz

100 ± 10 VAC, 1.5 A maximum 117 ± 12 VAC, 1.5 A maximum 234 ± 23 VAC, 0.75 A maximum

Factory Set

}

D Series Syringe Pumps

Section 1 Introduction

LINE VOLTAGE NOISE TOLERANCE 1.7

DIMENSIONS PUMP CONTROLLER

WEIGHT PUMP CONTROLLER

FLOW RATE RANGE See Figure 1-1.

FLOW RATE ACCURACY

FLOW RATE DISPLAY RESOLUTION 1.0 µl/min

ANALOG OUTPUT ACCURACY

DISPLACEMENT RESOLUTION 16.63 nl /step

REFILL TIME 2.5 minutes

REFILL OR DEPRESSURIZATION RATE 1.0 µl/min to 107 ml/min at any pressure from 0 to 517.1 bar

PRESSURE RANGE 0.6895 to 517.1 bar

PRESSURE ACCURACY ± 0.5% of full scale at constant temperature

PRESSURE REPEATABILITY

ZERO PRESSURE DRIFT ± 0.25% of full scale within 48 hours at constant temperature

PRESSURE DISPLAY RESOLUTION 6.895 kPa

 nominal rms line voltage, 10 µsecond pulses, any phase

angle, random or repetitive

Width: 27.18 cm 27.18 cm Depth: 46.74 cm 30.48 cm Height: 101.09 cm 13.59 cm

32.8 kg 2.96 kg

± 0.5% (maximum 0.50 µl/min seal leakage)

± 1% of selected range

± 0.5% of full scale within 48 hours at constant temperature

AMBIENT TEMPERATURE RANGE 5 to 40°C

TEMPERATURE DRIFT ± 0.15% of full scale/°C

HUMIDITY 95% maximum

DEAD (HEADSPACE) VOLUME

POLLUTION DEGREE 2

INSTALLATION CATEGORY II

MAXIMUM ALTITUDE 2000 m

a. Underwriters Laboratories (UL) has certified all D Series Pumps with the exception of the 100 VAC versions.

b. Using water at 137.9 bar and a temperature controlled environment at 30°C.

c. The analog output is an optional accessory.

d. Pressure repeatability specification is based upon re-zeroing pressure transducer every 48 hours. Refer to sub-section

ZERO PRESS in Section 3 of the manual for re-zeroing procedure.

e. Volume in and above the piston seal, head clearance at automatic shutoff, and inlet and outlet ports to the fittings.

2.10 ± 0.020 ml

1-7

D Series Syringe Pumps Section 1 Introduction

Table 1-7 500D Technical Specifications

CYLINDER CAPACITY 507.38 ml

POWER REQUIREMENTS

(Mains voltage line cord is a “Disconnect Device”)

LINE FREQUENCY 50 or 60 Hz

100 ± 10 VAC, 1.5 A maximum 117 ± 12 VAC, 1.5 A maximum 234 ± 23 VAC, 0.75 A maximum

Factory Set

}

LINE VOLTAGE NOISE TOLERANCE 1.7

DIMENSIONS PUMP CONTROLLER

WEIGHT PUMP CONTROLLER

FLOW RATE RANGE See Figure 1-2.

FLOW RATE ACCURACY

FLOW RATE DISPLAY RESOLUTION 1.0 µl/min

ANALOG OUTPUT ACCURACY

DISPLACEMENT RESOLUTION 31.71 nl /step

REFILL TIME 2.5 minutes

REFILL OR DEPRESSURIZATION RATE 1.0 µl/min to 204 ml/min at any pressure from 0 to 258.6 bar

PRESSURE RANGE 0.6895 to 258.6 bar

PRESSURE ACCURACY ± 0.5% of full scale at constant temperature

PRESSURE REPEATABILITY

ZERO PRESSURE DRIFT ± 0.25% of full scale within 48 hours at constant temperature

PRESSURE DISPLAY RESOLUTION 6.895 kPa

 nominal rms line voltage, 10 µsecond pulses, any phase

angle, random or repetitive

Width: 27.18 cm 27.18 cm Depth: 46.74 cm 30.48 cm Height: 102.36 cm 13.59 cm

33.25 kg 2.96 kg

± 0.5% (maximum 1.0 µl/min seal leakage)

± 1% of selected range

± 0.5% of full scale within 48 hours at constant temperature

AMBIENT TEMPERATURE RANGE 5 to 40°C

TEMPERATURE DRIFT ± 0.15% of full scale/°C

HUMIDITY 95% maximum

DEAD (HEADSPACE) VOLUME

POLLUTION DEGREE 2

INSTALLATION CATEGORY II

MAXIMUM ALTITUDE 2000 m

a. Underwriters Laboratories (UL) has certified all D Series Pumps with the exception of the 100 VAC versions.

b. Using water at 137.9 bar and a temperature controlled environment at 30°C.

c. The analog output is an optional accessory.

d. Pressure repeatability specification is based upon re-zeroing pressure transducer every 48 hours. Refer to sub-section

ZERO PRESS in Section 3 of the manual for re-zeroing procedure.

e. Volume in and above the piston seal, head clearance at automatic shutoff, and inlet and outlet ports to the fittings.

4.00 ± 0.020 ml

1-8

Table 1-8 500HPx Technical Specifications

CYLINDER CAPACITY 507.38 ml

POWER REQUIREMENTS

(Mains voltage line cord is a “Disconnect Device”)

LINE FREQUENCY 50 or 60 Hz

100 ± 10 VAC, 5A maximum 117 ± 12 VAC, 5A maximum 234 ± 23 VAC, 2.5A maximum

Factory Set

}

D Series Syringe Pumps

Section 1 Introduction

LINE VOLTAGE NOISE TOLERANCE 1.7

DIMENSIONS PUMP CONTROLLER

WEIGHT

FLOW RATE RANGE See Figure 1-3.

FLOW RATE ACCURACY

FLOW RATE DISPLAY RESOLUTION 1.0 µl/min

ANALOG OUTPUT ACCURACY

DISPLACEMENT RESOLUTION 25.36 nl /step

REFILL TIME 1.25 minutes

REFILL OR DEPRESSURIZATION RATE 1.0 µl/min to 408 ml/min at any pressure from 0 to 344.7 bar

PRESSURE RANGE 0.6895 to 344.7 bar

PRESSURE ACCURACY ± 0.5% of full scale at constant temperature

PRESSURE REPEATABILITY

ZERO PRESSURE DRIFT ± 0.25% of full scale within 48 hours at constant temperature

PRESSURE DISPLAY RESOLUTION 6.895 kPa

 nominal rms line voltage, 10 µsecond pulses, any phase

angle, random or repetitive

Width: 27.18 cm 27.18 cm Depth: 46.74 cm 30.48 cm Height: 102.36 cm 13.59 cm

PUMP CONTROLLER

42.64 kg 2.96 kg

± 0.5% (maximum 1.0 µl/min seal leakage)

± 1% of selected range

± 0.5% of full scale within 48 hours at constant temperature

AMBIENT TEMPERATURE RANGE 5 to 40°C

TEMPERATURE DRIFT ± 0.15% of full scale/°C

HUMIDITY 95% maximum

DEAD (HEADSPACE) VOLUME

POLLUTION DEGREE 2

INSTALLATION CATEGORY II

MAXIMUM ALTITUDE 2000 m

a. Underwriters Laboratories (UL) has certified all D Series Pumps with the exception of the 100 VAC versions.

b. A team lift is recommended when moving this instrument.

c. Using water at 137.9 bar and a temperature controlled environment at 30°C.

d. The analog output is an optional accessory.

e. Pressure repeatability specification is based upon re-zeroing pressure transducer every 48 hours. Refer to sub-section

ZERO PRESS in Section 3 of the manual for re-zeroing procedure.

f. Volume in and above the piston seal, head clearance at automatic shutoff, and inlet and outlet ports to the fittings.

10.45 ± 0.65 ml

1-9

D Series Syringe Pumps Section 1 Introduction

Table 1-9 1000D Technical Specifications

CYLINDER CAPACITY 1015.0 ml

POWER REQUIREMENTS

(Mains voltage line cord is a “Disconnect Device”)

LINE FREQUENCY 50 or 60 Hz

100 ± 10 VAC, 1.5 A maximum 117 ± 12 VAC, 1.5 A maximum 234 ± 23 VAC, 0.75 A maximum

Factory Set

}

LINE VOLTAGE NOISE TOLERANCE 1.7

DIMENSIONS PUMP CONTROLLER

WEIGHT

FLOW RATE RANGE See Figure 1-4.

FLOW RATE ACCURACY

FLOW RATE DISPLAY RESOLUTION 1.0 µl/min

ANALOG OUTPUT ACCURACY

DISPLACEMENT RESOLUTION 25.38 nl /step

REFILL TIME 2.5 minutes

REFILL OR DEPRESSURIZATION RATE 1.0 µl/min to 408 ml/min at any pressure from 0 to 137.9 bar

PRESSURE RANGE 0.6895 to 137.9 bar

PRESSURE ACCURACY ± 0.5% of full scale at constant temperature

PRESSURE REPEATABILITY

ZERO PRESSURE DRIFT ± 0.25% of full scale within 48 hours at constant temperature

PRESSURE DISPLAY RESOLUTION 6.895 kPa

 nominal rms line voltage, 10 µsecond pulses, any phase

angle, random or repetitive

Width: 27.18 cm 27.18 cm Depth: 46.74 cm 30.48 cm Height: 102.36 cm 13.59 cm

PUMP CONTROLLER

38.5 kg 2.96 kg

± 0.5% (maximum 1.5 µl/min seal leakage)

± 1% of selected range

± 0.5% of full scale within 48 hours at constant temperature

AMBIENT TEMPERATURE RANGE 5 to 40°C

TEMPERATURE DRIFT ± 0.12% of full scale/°C

HUMIDITY 95% maximum

DEAD (HEADSPACE) VOLUME

POLLUTION DEGREE 2

INSTALLATION CATEGORY II

MAXIMUM ALTITUDE 2000 m

a. Underwriters Laboratories (UL) has certified all D Series Pumps with the exception of the 100 VAC versions.

b. A team lift is recommended when moving this instrument.

c. Using water at 137.9 bar and a temperature controlled environment at 30°C.

d. The analog output is an optional accessory.

e. Pressure repeatability specification is based upon re-zeroing pressure transducer every 48 hours. Refer to sub-section

ZERO PRESS in Section 3 of the manual for re-zeroing procedure.

f. Volume in and above the piston seal, head clearance at automatic shutoff, and inlet and outlet ports to the fittings.

11.0 ± 0.7 ml

1-10

260D Flow Rate Range

110

107

100

Flow Rate (ml/min)

0 25 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 500

Pressure (bar)

Flow Rate (ml/min) Max. Pressure (bar)

0.001 to 90 517.1

90 to 107 (107.23 ml/min – flow rate)

D Series Syringe Pumps

Section 1 Introduction

525

517.1

30

Figure 1-1 260D flow rate range

204

200

180

170

160

140

120

100

Flow Rate (ml/min)

0 20 40 60 80 100 120 140 160 180 200 220 240

Flow Rate (ml/min) Max. Pressure (bar)

0.001 to 170 258.6

170 to 204 (204.9 ml/min – flow rate)

500D Flow Rate Range

Pressure (bar)

258.6

7.4

Figure 1-2 500D flow rate range

1-11

D Series Syringe Pumps Section 1 Introduction

425

408

400

375

350

340

325

300

275

250

225

200

175

150

125

Flow Rate (ml/min)

100

0 25 50 75 100 125 150 175 200 225 250 275 300 325 350 375

Flow Rate (ml/min) Max. Pressure (bar)

500 HPx Flow Rate Range

Pressure (bar)

344.7

0.001 to 340 344.7

340 to 408 (409.8 ml/min – flow rate)

Figure 1-3 500 HPx flow rate range

425

408

400

375

350

340

325

300

275

250

225

200

175

150

125

Flow Rate (ml/min)

100

0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150

1000D Flow Rate Range

Pressure (bar)

4.985

137.9

Flow Rate (ml/min) Max. Pressure (bar)

0.001 to 340 137.9

340 to 408 (409.8 ml/min – flow rate)

Figure 1-4 1000D flow rate range

1-12

1.9756

D Series Syringe Pumps

Section 1 Introduction

1.3 Unpacking After removing the pump, controller, and accessories from the

Teledyne Isco

Customer Service Dept. P.O. Box 82531 Lincoln, NE 68501 USA

Phone: (800) 228-4373

(402) 464-0231

FAX: (402) 465-3022

E-mail: IscoInfo@teledyne.com

shipping carton, examine them for signs of shipping damage. Be sure no internal parts have shaken loose in transit. If there is any shipping damage, file a claim with the delivering carrier immediately.

Compare the contents of the boxes with the enclosed packing slip. If there are shortages, contact Teledyne Isco immediately.

1-13

D Series Syringe Pumps Section 1 Introduction

1.4 Controls and Indicators

The pump controller regulates all pumping functions. It is designed to sit on top of the pump base, but may be located elsewhere, according to safety and convenience. Programming and setup are performed using the keypad on the front panel. The controller front panel is shown in Figure 1-5 and described in Table 1-10. Table 1-11 explains the key functions.

The rear panel of the pump controller contains several input and output connectors, explained in Table 1-12, and shown in Figure 1-6.

The only operational control on the pump itself is the power switch, shown in Figure 1-8. The rear panel has several connectors, described in Table 1-13, and shown in Figure 1-7.

Figure 1-5 Pump controller key functions

Table 1-10 Pump Controller Front Panel Label

Item No. on Figure 1-5

1 On/Standby switch Turns instrument off and on.

2 Programming keypad Used to program controller.

3 Softkeys Labeled A, B, C, and D; used to select menu items

4 Liquid crystal display 40 Characters

1-14

Connector Description

(Does not disconnect power.)

displayed directly above them.

 4 line.

Table 1-11 Pump Controller Key Functions

Key Description

D Series Syringe Pumps

Section 1 Introduction

ON/STANDBY Two-position toggle switch turns controller on and activates drive motor

A, B, C, D Softkeys; used to select displayed options.

PRGM GRAD Program gradient: Puts pump in gradient mode and accesses the soft-

CONST PRESS Constant pressure: Puts pump in constant pressure mode.

CONST FLOW Constant flow: Puts pump in constant flow rate mode.

STORE Stores the current program in nonvolatile memory and exits program-

LIMITS Displays and allows changes to the maximum and minimum pressure

RAPID PRESS Rapid pressure: Allows rapid pressurization to the stable pressure point

RECALL Replaces the current program with one recalled from nonvolatile

ACC CTRL Accessory control: Manually operates accessories such as valves.

ZERO PRESS Zero pressure: Sets pressure display to zero. Active only from -750 to

to maintain position. Standby disables the drive motor and halts the controlling processor.

key driven gradient programming.

ming mode.

and flow rate limits.

and then switches automatically to constant flow. (Available in constant flow mode only.) NOTE: This feature is automatic, i.e. RAPID PRESS is pressed only once and the user does not enter a pressure; although, entering a target pressure may speed equilibration.

memory.

+750 psi.

CLEAR ENTRY Clear the last digit entered from the numeric key.

MENU Accesses software to set operational modes, units, and other optional

DISP Activates dispense mode (refer to Section 3.10.3).

HOLD Freezes the program clock. The unit will continue at the current gradient

REFILL Turns on pump drive motor to move piston downward at a rate

RUN Turns on pump drive motor to move piston upward in a previously pro-

STOP Stops the drive motor.

ENTER Enters selected values to memory.

NUMBER KEYS These keys are used to make menu selections and enter values when

parameters.

previously programmed.

grammed mode, such as “CONSTANT FLOW” or “CONSTANT PRESSURE.”

setting parameters.

Note

A more in-depth explanation of the keypad is provided in Section 3 Basic Programming and Operation, under Section 3.8 Front Panel Keys.

1-15

D Series Syringe Pumps Section 1 Introduction

Figure 1-6 Pump controller rear panel connectors

Table 1-12 Pump Controller Rear Panel Connectors

Item No. on Figure 1-6

Connector Description

1 PUMP D This connector is only used during multiple pump operation. The control

2 PUMP C This connector is only used during multiple pump operation. The control

3 PUMP B This connector is only used during multiple pump operation. The control

4 PUMP A This plug connects the control cable from the pump rear panel. This connec-

5 ACCESSORY These terminals allow connection of input and output signals

6 RS-232/RS-485 This serial port connector may be used with an RS-232 or RS-485 cable to

7 USB/ETHERNET

cable from the rear panel of the fourth pump is attached to this connector.

cable from the rear panel of the third pump is attached to this connector.

cable from the rear panel of the second pump is attached to this connector.

tion should be secured with the thumbscrews. IMPORTANT: The pump A connector is the only input power connector on

the rear panel of the controller. During single pump operation, the pump must be attached to this connector to supply power to the controller.

WARNING

Do not connect or disconnect the control cable when the pump is connected to the mains voltage.

(such as analog controls and external RUN/STOP).

place the pump under remote control. Refer to Section 8 for RS-232 and Section 7 for RS-485 pin connections.

Optional circuit provides USB and Ethernet functionality. Refer to Section 8 for USB pin connections and Section 7 for Ethernet pin connections

1-16

D Series Syringe Pumps

Section 1 Introduction

8 4-20mA OUTPUT

Optional circuit provides 4-20mA current loop output and additional digital inputs and outputs.

ANALOG OUTPUT

ELECTRIC VALVES

12-CH OUTPUT

9 DIGITAL I/O

ANALOG OUTPUT

Optional circuit provides flow rate and volume outputs. See Section 3.9.

Optional circuit provides motor drive for valve operation.

Additional circuit provides flow rate, pressure, and volume outputs.

Optional circuit provides additional digital inputs and outputs.

Optional circuit provides flow rate and volume outputs. See Section 3.9.

10 SERIAL TAG This tag indicates the serial number of the instrument.

11 CHASSIS GROUND Ground point for high static or remote controller installations.

a. Connectors based on installed optional circuit board configuration.

60-1242-306

Figure 1-7 Pump rear panel connectors

Table 1-13 Pump Rear Panel Connectors

Item No. on Figure 1-7

1 Pressure outputs Two red binding post/banana jacks providing pressure output voltages.

2 Ground A black binding post/banana jack providing a connection to circuit

3 Pressure transducer The pressure transducer cable must be plugged in for the pump

4 Control cable This cable connects the pump to the controller.

5 Mains IEC power connector with EMI filter.

Connector Description

Pump Left Jack Right Jack

65D 1.0 V/2000 psi 0.1 V/2000 psi 30D 1.0 V/3000 psi 0.1 V/3000 psi All others 1.0 V/1000 psi 0.1 V/1000 psi

common.

to operate.

VDC/psi VDC/psi

1-17

D Series Syringe Pumps Section 1 Introduction

6 External Fuses * Limits pump current drawn from main power supply.

7 Chassis ground Ground point for high static installations.

Replace with same type: (“T” time delay fuses)

1 - 2.0 Amp for 100/117 volt operation 2 - 1.0 Amp for 234 volt operation

For 500HPx Pumps:

1 - 7.5 Amp for 100 volt operation 1 - 6.25 Amp for 117 volt operation 2 - 3.2 Amp for 234 volt operation

For 30D Pumps:

1 - 3.2 Amp for 100/117 volt operation 2 - 1.6 Amp for 234 volt operation

To remove, rotate cap counterclockwise.

* Internal fuses not

replaceable by the operator

F101 4.0 Amp “T” F102, F104 1.5 Amp “T” F103 0.75 Amp “T”

For 500HPx/30D F101 6.25 Amp “T”

Figure 1-8 Pump front panel controls

Table 1-14 Pump Front Panel

Item No. on Figure 1-8

1 Mains power switch Disconnects power from the pump circuits for setup changes, such as

Connector Description

connecting the controller.

“1” = mains power is applied to the pump circuitry. “0” = mains power is removed from the pump circuitry.

1.5 Electrical Connections The pump controller may be placed on top of the pump, or

wherever safety and convenience dictate. Power is supplied to the pump controller through the control cable.

1-18

D Series Syringe Pumps

Section 1 Introduction

CAUTION

All connections between the pump and controller should be made BEFORE the pump is connected to mains power.

1. Connect the pressure transducer cable (which originates from the top of the pump cylinder) to the nine pin sub-D PRESSURE TRANSDUCER connector on the pump rear panel (Figure 1-7). Be sure to tighten the thumbscrews.

2. Connect the control cable (which originates from the first or only pump rear panel) to the PUMP A connector on the rear panel of the controller (Figure 1-6), and tighten the thumbscrews. This cable must be plugged into the PUMP A connector.

Note

There are four PUMP connectors on the rear of the controller. Only the PUMP A connector is wired to supply power to the controller; therefore, one pump must be attached to this connector.

3. Check the serial number tag to make sure the voltage rating of the pump is correct.

4. Connect the line cord to the MAINS connector on the back of the pump.

1.5.1 Cabling For all systems, connect the control cable of the pump module (Pump A for multiple-pump systems) to the connector labeled PUMP A on the rear panel of the controller. Subsequent pumps in multiple-pump systems can be connected to controller connectors PUMP B, PUMP C, and PUMP D.

Note

The transducer cable (refer to Figures 1-9 and 1-10) must be plugged into the PRESSURE TRANSDUCER connector on the rear panel of the pump module for the pump to operate.

Cable part numbers and lengths are listed in the following chart:

USB and TCP/IP use cable package 60-1247-168. Pump controller connect cable (see Figure 1-10). 7 ft (2.1m) Pump module transducer cable (see Figure 1-9). 4 ft (1.2m) 30D/65D Pump module transducer cable

(attached to pump)

4.5 ft (1.4 m) 69-1244-413

1-19

D Series Syringe Pumps Section 1 Introduction

Extension cable for pump controller connect

RS232 Cable for computer serial control of a single controller. 9 pin to 25 pin.

RS232 Cable for computer serial control of two controllers. 9 pin to two 25 pin.

RS232 Extension cable for computer serial control of three or more controllers. Connects to above cable. 25 pin to two 25 pin.

Hazloc pumps:

10 ft (3m) 50 ft (15.24m)

D Series Pumps only:

10 ft (3m) 50 ft (15.2m)

6 ft (1.8m) 480-7996-00

6 ft (1.8m) 68-1020-198

68-1020-505 68-1020-506

68-1020-210 68-1020-214

Transducer Cap assembly for the 30D/65D

Figure 1-9 Syringe pump cylinder cap: Attach to pressure

transducer connector on back of pump.

6 ft (1.8m) 68-1020-180

Transducer cable, 6ft (1.8m)

1-20

CAUTION

The pressure transducer cable length is always 6 feet (1.8m). Never attempt to extend the length of this cable.

D Series Syringe Pumps

Section 1 Introduction

Controller

Pump

Pressure Transducer

connector

Figure 1-10 Connecting the pump and controller

CAUTION

Never make any cable connections while the pump is powered ON.

1.6 Preliminary Checkout After the electrical connections have been completed, follow this

brief test of the pump’s operation:

Note

Preliminary checkout of the pump is performed without fluid in the pump.

Figure 1-11 Status Screen

If adding a new pump to the controller, a hard and soft reset need to be completed. See Section 9.4.

CAUTION

Always maintain clearance behind the pump such that the MAINS power cord can be unplugged at any time.

1. Before connecting the line cord, make sure the voltage rating on the serial tag matches your outlet’s voltage. Plug in the line cord. Turn the pump power switch ON. Turn the controller ON/STANDBY switch ON.

2. The display will briefly show the software revision on the first line; the pump model(s) connected to the controller.

PUMP CONTROLLER ISCO, INC. REV ____

SERIAL CHANNEL X, UNIT ID 6, Baud = 9600

CD Jun 16, 2011

1-21

D Series Syringe Pumps Section 1 Introduction

3. Check the upper left corner of the controller screen. The current pump mode will appear in a two-letter abbreviation, e.g. CF for constant flow. This will be followed by a lowercase letter indicating the current pump, e.g. “a” indicates that pump A is the current pump. The current pump is the one for which parameters are being set.

a. If a pump other than pump A is currently selected:

In the lower right corner of the screen, directly over softkey D, are the words “SELECT PUMP.”

Press MENU >2>Ato select pump A. The display will

automatically switch to the run screen, showing “a” in the upper left corner.

4. Press MENU to display Menu 1.

Figure 1-12 Menu 1

1. UNITS

2. SELECT PUMP

3. REFILL

4. POWER FAILURE [STOP]

5. SYSTEM RESET

6. DISPLAY CONTRAST

RETURN

5. Press 1 for UNITS.

6. In the Units menu, select the desired units to display for pres-

sure. For example, press 3

to select PSI for the pressure

units.

7. In the Units menu, select the desired units to display for flow.

For example, press

5 to select ML/MIN for the flow units.

The first line of the display will show the selected units.

8. Press PREVIOUS (D) to return to Menu 1.

9. Press RETURN (D), to exit Menu 1.

10. Press CONST FLOW to set the pump mode to constant flow. CFa will be displayed in the upper left corner of the screen.

11. Press FLOW RATE (A). The words ENTER FLOW RATE should flash on the display. Use the numeric keys to enter a flow rate of 10 ml/min and press ENTER.

Note

If you make an error, press CLEAR ENTRY to delete it.

1-22

12. Press RUN. Observe the flow rate displayed on the first line. After a few moments, the setpoint and flow rate display should match.

13. Once the setpoint and flow rate match, press STOP.

a. If three or fewer pumps are connected, you will be

prompted to press A to stop pump A, B to stop pump B, C to stop pump C, or D to stop all pumps.

D Series Syringe Pumps

Section 1 Introduction

PRESS RUN KEY TO ESCAPE PRESS ‘ALL PUMP’ OR STOP KEY TO STOP ALL

Figure 1-13 Three pump stop menu

Figure 1-14 Four pump stop menu

PUMP A

PUMP B PUMP C

ALL PUMP

b. If four pumps are connected to the controller, you will

be prompted to press A to stop pump A, B to stop pump B, C to stop pump C, D to stop pump D, or STOP to stop all pumps.

PRESS RUN KEY TO ESCAPE PRESS STOP KEY TO STOP ALL

PUMP A

PUMP B PUMP C

PUMP D

If you encountered any problems during the preliminary checkout, please contact the Teledyne Isco Service Department. The number is (800) 775-2965 or (402) 464-0231.

Note

In the event of an emergency, turn off the controller switch. This will cause all pumps to immediately stop.

1-23

D Series Syringe Pumps Section 1 Introduction

1-24

D Series Syringe Pumps

Section 2 Fluid System Connections and Accessories

2.1 Introduction This section discusses general fluid system connections, and the

installation of fluid connection accessories, temperature and pressure control accessories, and optional kits and attachments.

DANGER

RISK OF INJURY. THIS EQUIPMENT PRODUCES HAZARDOUS PRESSURES. USE APPROPRIATE

TUBING AND CONNECTIONS AS INSTRUCTED.

Note

When operating at flow rates at or below 500 µl/min, it is strongly suggested that an insulating cover or temperature control jacket be installed. See Sections 2.4.1 and 2.4.2.

2.2 Fluid System Connections

2.2.1 Ports There are two ports in the pump standard cylinder cap. Either

Standard port information

• 1000D -14" NPT • 500D -18" NPT

• 65DM, 100DM/DX, 260D -18" Valco • 65D/30D-14" F250C

• 500HPx -38" NPT

All D Series pump models connect similarly, but with varying port sizes. Take care to follow the tips provided to ensure safety and proper operation.

port can serve as the inlet or outlet. As shown in Figure 2-1, you may plug one port and use a single port as both the inlet and outlet.

Note

Custom caps are available with different port configurations and sizing. Contact the factory for details.

2-1

D Series Syringe Pumps Section 2 Fluid System Connections and Accessories

Pressure

transducer cap

Nut &

Pressure Transducer

Nut

Nut & Collar

Plug

Tubing to valve

Ferrule

Port

Tubing to valve

Figure 2-1 Liquid system plumbing connections (30D on

Left)

2.2.2 Installation Tips • Be sure to keep the tubing as straight as possible at the end, as this will make it easier to install the ferrules.

• Be sure to cut the ends of the tubing squarely.

• Do not leave burrs on the ends of the tubing.

• When installing ferrules on the tubing, be sure the tubing extends beyond the ferrule to allow for proper crimping.

• If the connection leaks, retighten fittings.

• Push the tubing completely into the port before tightening the nut.

• When connections are made to the cylinder cap, the pressure reading may be affected. If the pressure no longer reads zero, release all pressure inside the pump, then press ZERO PRESSURE on the front panel of the controller to readjust.

Plug

Port

2.2.3 Tubing Requirements The tubing must be cut squarely to prevent possible problems. Square ends are easier to insert through the ferrule, and will decrease dead volume.

Purchased cut tubing Electrochemically machined steel tubing should be used

throughout the plumbing system. Electrochemically machined tubing has flat, burr-free ends, and is free of cutting residues. This tubing is available pre-cut through many chromatographic supply distributors in assorted lengths.

Cutting the tubing A less desirable alternative is to purchase a tubing cutter

designed to handle steel tubing. For quick fixes, the tubing may also be cut by hand.

2-2

D Series Syringe Pumps

Section 2 Fluid System Connections and Accessories

CAUTION

Wear goggles to perform the following procedure.

Tools required: Fine jewelers file, goggles, and two pairs of pliers

1. Using the jewelers file, score the tubing around its entire circumference.

2. Secure the tubing with pliers on either side of the score line with approximately 1.5mm between each pair of pliers and the score line. Do not squeeze the tubing too tightly, as this will flatten or deform its exterior.

3. Bend the tubing back and forth to crack it at the score line.

4. It may be necessary to deburr the outer tubing ends with the file. Make sure the tubing ends are clean and the inner bore is clear before installing the cut tube.

Note

It is often impossible to remove a burr that blocks the inner bore.

2.2.4 Accessory Packages The accessory packages for all pumps contain fittings that allow you to attach tubing to the pump (refer to Figure 2-1), as well as appropriately sized cylinder seals. Note that the tubing is not included.

Installation 1. Slide first the nut and then the ferrule over the tubing.

2. Push the tubing all the way into the port.

3. Hold the tubing in place and tighten the nut.

Table 2-1 D Series Syringe

Pump Accessory Packages

Pump Model Part Number

1000D 60-1249-103

500D/HPx 60-1249-016

65D 60-1249-024

30D 60-1249-131

260D 60-1249-012

100DM/DX 60-1249-015

65DM 60-1249-028

2-3

D Series Syringe Pumps Section 2 Fluid System Connections and Accessories

2.2.5 Installing the1/16"

Fittings (260D, 65DM, and 100DM/DX only)

Refer to Figure 2-1

1. Loosen the116" nut on the adapter until it is held by a few threads.

2. Insert the adapter into the port and finger-tighten the fittings.

3. Retighten the116" nut until it is also finger-tight.

4. Tighten the18" nut with a wrench to crimp the ferrule.

5. Insert the116" tubing through the116" nut until it stops.

6. Tighten the116" nut.

/8"Ferrule

Figure 2-21/16" Tubing adapter for 30D/65D pumps

Note

It is recommended that after tightening, the fittings be removed and examined.

/8"Nut

Nut

16"

Ferrule

16"

Tubing

16"

F250 Fitting/Connection Refer to Figure 2-3 for components and fittings for the 30D/65D.

Figure 2-3 30D/65DFitting

Gland nut

Collet grips tubing here for anti-vibration

support

Collar

Line contact sealing

Slotted collet

Valve or fitting body

2-4

D Series Syringe Pumps

Section 2 Fluid System Connections and Accessories

2.2.6 Draining Overflow The drip tray outlet on the pump cylinder provides a means of draining fluid from seal leakage. Use the14" ID flexible tubing included with the accessory package to divert the leakage away from the pump.

To install the drain tube, push one end of the tubing over the end of the drip tray outlet, as shown in Figure 2-4.

Note

The 1000D has two drain tube outlets, one for the drip tray, and one for the splash pan, located at the bottom of the pump (see Figure 2-5). The 1000D also includes a wash gland as a standard feature (see the following section for details). The wash gland tubes can also be used as lines for draining away fluid.

Figure 2-4 Drain tube installation

2.2.7 Cylinder Washing:

Wash Gland

Drip tray outlet

A thin film of liquid wets the inside of the cylinder each time the piston travels up the cylinder. The lowest flow rates are conducive to the most abrasive or corrosive pumping environments, as the deposited film remains on the inside of the cylinder wall for the longest time.

The Model 1000D syringe pump is equipped for cylinder washing, with two wash gland, as shown in Figure 2-5 on the following page.

A small pump can be used to deliver the wash fluid through one of the two tubes to rinse the cylinder and seals. The second tube drains the wash fluid to waste. If the system is configured to recirculate the wash fluid, ensure that you change the wash fluid at regular intervals.

Select a wash fluid that will best flush the cylinder of any residue left by the pumped fluid, yet will not damage the seals.

/8" tubes on the back to feed and drain the

2-5

D Series Syringe Pumps Section 2 Fluid System Connections and Accessories

The pressure in the wash gland and line should NEVER exceed the system pressure or the wash pressure limit as labeled on the pump. Units with a wash pressure limit label can be damaged if the pressure is exceeded.

Note

If the primary pump seal fails, the pressure of the delivery fluid will be exerted on the secondary (wash gland) seal. During operation, the wash gland outlet should always be uncapped and routed for either recirculation or drainage.

Detailed information about pumping salt solutions and brines is available in technical bulletin TB04 Pumping Salt Solutions and

Brines.

Drip tray outlet

CAUTION

Splash pan outlet

Wash gland

tubes

1/8” Wash Gland

Tubes

1/8” Tubing*

Pump*

Outlet Fluid*

Figure 2-5 Drain tube installation and wash gland connection

2-6

Inlet Fluid*

* User-supplied

D Series Syringe Pumps

Section 2 Fluid System Connections and Accessories

Note

Wash gland options are available for the 500D syringe pump, with1/16" wash tubes. Contact Teledyne Isco for more information.

2.2.8 Cylinder Washing:

Nitrogen Purge

To purge with nitrogen 1. Attach gas supply by slipping the plastic tube over the

All pumps that do not have a wash gland are equipped with a purge connector tube near the top of the pump body. The purge connector enables the pump cylinder beneath the piston to be purged with nitrogen, which can increase useful cylinder life by flushing residue from the system. Figure 2-6 shows a typical connection to the purge tube on the back of the pump.

purge connector, as shown below.

2. Regulate the nitrogen supply to slightly above atmospheric pressure.

Figure 2-6 Purge connector installation

Close-up view of nitrogen

purge tube

2-7

D Series Syringe Pumps Section 2 Fluid System Connections and Accessories

2.2.9 Flushing Pumps with External Transducers

When changing pumped liquids in the Model 65D, 30D or other models with external transducers, flush the pump to prevent cross-contamination or difficulties with incompatible fluids.

In comparison to other D-Series pumps, those with external transducers have a greater dead volume space due to transducer and tubing differences. This dead volume space increases the possibility of residual liquid being held in the pump. Also, the external transducer may retain residual liquid. Dead volumes are listed in the specification tables found in Section 1.

To flush the pump, remove the transducer and its tubing from the top of the pump.

CAUTION

Never immerse the transducer in solvent.

Using nitrogen, blow out any liquid that remains inside the cylinder, transducer port, and tubing.

WARNING

Liquids expelled by compressed gasses may cause injury. Wear eye protection.Certain liquids also may requireother personal protective equipment. Refer to the applicable Material Safety Data Sheet (MSDS) for more information.

2.3 Fluid Connection Accessories

2.3.1 Manual Refill Kit The optional manual refill kit provides a high pressure, two-way

Kit installation 1. To attach the two-way valve to the pump housing, use the

The optional accessories discussed in this section are used to make fluid connections from the pump(s) to another apparatus.

When making fluid connections that use ferrules, be sure to use the ferrules provided in the kit. Push the tubing completely into the connector and finger-tighten. Then tighten with a wrench to clamp the ferrules onto the tubing.

valve that connects to the pump inlet to a fluid reservoir. The kit contains all tubing and hardware necessary for valve installation. Kit components and connections are shown in Figure 2-7.

valve spacer block and screws provided.

a. For the 500D pump, screw the male adapter into the

inlet port of the pump.

2. Connect the pre-bent stainless steel tubing from the valve to the pump inlet. Use the nut and ferrule to connect the tubing at the inlet and the valve fittings to connect the tubing at the valve.

3. Connect the PTFE refill tubing (with the filter) to the port of the two-way valve, using the nuts and ferrules supplied.

2-8

nut, ferrule

D Series Syringe Pumps

Section 2 Fluid System Connections and Accessories

Note

When connecting to pressurized sources in supercritical fluid applications, use the stainless steel tubing without a filter. An in-line filter is contained in the CO2connection package (refer to Section 2.3.3).

Table 2-2 Manual Refill Kits

Pump Model Part Number

1000D 68-1247-117

500D 68-1247-083

pre-bent

SST tubing

system

spacer bloc

65DM, 100DM/DX, 260D 68-1247-077

30D 60-1247-180

65D 68-1247-127

DANGER

PTFE tubing

stainless steel tubing

valve

filter

Figure 2-7 Refill kit installation

2.3.2 Manual Outlet Valve Kit

The optional manual outlet valve kit provides manual control of the pump outlet port by connecting a shutoff valve between the pump and the rest of the system.

Kit installation 1. Attach the two-way valve using the spacer block and pan-

RISK OF INJURY. THIS EQUIPMENT PRODUCES HAZARDOUS PRESSURES. PLEASE UTILIZE APPROPRIATE TUBING AND CONNECTIONS AS NOTED INTHE MANUAL.

head screws.

a. For the 500D pump, screw the male adapter into the

pump outlet.

2. Connect the pre-bent length of stainless steel tubing to the outlet port on the pump using the nut and ferrule. Connect the other end to the top port on the valve using the valve fitting.

Note

For the 500D, this piece of tubing should be cut to the proper length for connection to your system. Due to the wide variety of applications for this model, fittings to connect the tubing to your system are not provided in the kit.

2-9

D Series Syringe Pumps Section 2 Fluid System Connections and Accessories

Note

When nuts are torqued to the cylinder cap ports, the pressure reading may be affected. If the pressure no longer reads zero, release the pressure in the cylinder and press ZERO PRESS.

3. Use the valve fittings to attach stainless steel tubing to the top port of the two-way valve.

a. For models 65DM, 100DM/DX, and 260D, connect the

reducing union to the other end of this tubing.

4. Connect the stainless steel tubing between the valve’s bottom port and your apparatus. Cut to the desired length.

Table 2-3 Manual Outlet Valve Kits

male adapter

500D only

nut, ferrule

Pump Model Part Number

1000D 68-1247-118

500D 68-1247-082

refill kit

stainless steel tubing

screws

assembly

valve

spacer

RISK OF INJURY. THIS EQUIPMENT

stainless steel

tubing

screws

stainless steel

tubing

(65DM, 100DM/DX, 260D)

system

valve

reducing union

Figure 2-8 Outlet valve package connection

2.3.3 CO2Cylinder Connection Package

PRODUCES HAZARDOUS PRESSURES. PLEASE UTILIZE APPROPRIATETUBING AND CONNECTIONS AS NOTED INTHE MANUAL.

For North American gas tank connections, the optional CO2connection package is available from Teledyne Isco.

Table 2-4 CO2Cylinder Connection Package 68-1247-043

Qty. Part Number Description

1 209-0161-63 Reducing connector

1 209-0161-16

1 209-0161-15 0.830-14 nut

65DM, 100DM/DX, 260D 68-1247-078

30D 60-1247-181

65D 68-1247-126

DANGER

8"to14"

4"  212" nipple

2-10

1 209-0161-17 PTFE washer

1 209-0161-64 In-line filter assembly

D Series Syringe Pumps

Section 2 Fluid System Connections and Accessories

Reducing connector

To Valve Inlet

To CO Supply

PTFE washer

Nipple

Nut

Inline filter frit assembly

Figure 2-9 CO2package installation

2.3.4 In-Line Filter Package The optional in-line filter package should be used when it is

important to filter flow exiting the pump. This package contains a 0.5 µm filter and116" tubing.

Note

This filter is not for use at pressures above 6,000 psi (414 bar).

Table 2-5 Optional in-line Filter Package 68-1247-011

Qty. Part Number Description

1 60-1243-231 1.5 m stainless steel tubing,

1 60-1243-232 0.3 m stainless steel tubing,

1 209-9012-17 In-line solvent filter with replacement frit, 0.5 micron filter

5 209-0094-07 Zero volume,

5 209-0094-08 Zero volume,

16" valve nut

16" valve ferrule

16"OD 0.020" ID

Installation 1. Attach the reducing adapter from the pump accessory

Figure 2-10 In-line filter package

package to the pump outlet, using the nut and ferrule provided. Or, connect to the reducing union of the outlet valve package.

2. Attach the116" - 1.5 m length of tubing to the reducing adapter. Cut to the desired length.

3. Then attach the in-line filter to the end of the tubing. The flow direction is indicated on the filter body.

4. Connect the remaining 0.3 m length of tubing between the in-line filter and the receiving device. Cut to the desired length.

In-line Filter

1.5 m Tubing Length

0.3 m Tubing Length

2-11

D Series Syringe Pumps Section 2 Fluid System Connections and Accessories

2.4 Temperature and Pressure Controls

2.4.1 Cylinder Insulating Cover

Some applications, such as those with very low flows, may require additional measures to maintain steady rates.

The optional insulating cover can reduce flow noise due to temperature fluctuations and improve pump performance at flow rates under 500 µl/min.

Note

The insulating cover is not available for Models 500HPx and 30D.

Part numbers:

• Insulating cover for 65D 60-5364-267

• Insulating cover for 65DM, 100DM/DX, and 260D 68-1247-081

• Insulating cover for 500D 68-1247-085

The cover consists of two pieces that fit over the cylinder, as shown in Figure 2-11.

Notches for

tubing

Side Latches

Notch for cable

Figure 2-11 Cylinder insulation cover

2-12

Section 2 Fluid System Connections and Accessories

Notches in the cover provide openings for the inlet and outlet tubing, and for the cable to the pressure transducer.

Installation 1. Install the back cover.

2. Route the cable around tubing through the appropriate tabs.

3. Install the front cover and secure the side latches.

D Series Syringe Pumps

2.4.2 Temperature Control Jacket

The optional cylinder temperature jacket assists in maintaining cylinder temperatures (–30 to 100°C) by circulating liquids, such as water or water/ethylene glycol solution, through the14" upper and lower hose connectors.

CO2 connection kit

Dip tube

T.C. Jacket

User-supplied

device

Circulating bath

(Extruder Reactor Rock Core Etc.)

Syringe Pump

Figure 2-12 System with temperature control jacket installed

The jacket is very useful for SFC applications where cylinder cooling facilitates filling with fluids such as liquid CO2.The jacket can also be used with a circulating temperature-controlled bath to keep the fluid inside the pump at a constant temperature. This may be necessary when operating at very low flow rates (below 500 µl/min), where temperature fluctuation can cause flow variations.

2-13

D Series Syringe Pumps Section 2 Fluid System Connections and Accessories

Table 2-6 Packages and Parts

Item Description Part Number

Temperature control jacket packages for:

Temperature control jacket assembly:

Key

32" socket

65D, 65DM,100DM, 100DX, 260D 30D 500D, 500HPx 500SP 1000D

65DM, 100DM, 100DX, 260D 30D 500D, 500HPx 1000D

68-1247-047 68-1267-001 68-1247-057 68-1247-115 60-5364-199

60-1248-053 60-1268-020 60-1248-099 60-1248-183

490-0031-54

Removing the pump cylinder for 30D

Inlet/outlet connector (2) 209-0161-00

Installation procedure 60-1242-183

O-ring (2):

100DM, 100DX, 260D 30D, 65D, 65HP, 500D,

202-2062-23

202-2062-11 500HPx 1000D

202-2062-35

Note

For the 30D pump remove the cylinder cap assembly ONLY. Do not remove the cylinder from the cylinder block.

1. Run the pump until the control display shows CYLINDER

EMPTY.

2. Remove the fluid fittings from the sides of the pump cap (Figure 2-13).

Transducer Connector

Figure 2-13 Fluid fittings

2-14

Fluid Fittings

D Series Syringe Pumps

Section 2 Fluid System Connections and Accessories

3. Run the pump until the display shows CYLINDER FULL.

Note

This will fill the pump with air to prevent spills later.

Figure 2-14 Secure screws with the

4. Toggle the S

TANDBY switch on the controller, turning off

the controller display.

5. Toggle the P

OWER switch on the pump to turn off the

pump.

6. Unplug the pump from the M

AINS power.

7. Disconnect the transducer connector from the top of the transducer (Figure 2-13) and position the cable out of the way.

8. Place the two parts of the C

YLINDER HOLDER (part of

Wrench Kit 60-1249-132) onto the pump cylinder just below the threads for the cylinder cap (approximately 1/4” (6 mm) below the cylinder cap) (Figure 2-14).

Cylinder holder

Cap screws

Hex wrench

/4” hex wrench

Note

If your pump has a cooling jacket installed, assemble the CYLINDER HOLDER below the cooling jacket.

9. Install the 5/16-18 X 1” Cap Screws (supplied with the

Wrench Kit) to hold the two parts of the CYLINDER HOLDER in place (Figure 2-14).

10.Tighten the cap screws referred to in step 9 above with

the1/4” hex wrench (supplied with the Wrench Kit) as shown in Figure 2-14, securing the CYLINDER HOLDER to

the pump cylinder.

11.Using the two wrenches (supplied with the Wrench Kit)

remove the cylinder cap assembly (Figure 2-15).

2-15

D Series Syringe Pumps Section 2 Fluid System Connections and Accessories

Figure 2-15 Removing the cylinder cap with wrenches

Cap assembly

Wrenches

Removing the pump cylinder

Refer to Figure 2-16 on the next page for the following steps.

1. Empty the cylinder.

Note

Be sure the piston is left fully extended.

2. Turn the pump’s mains power switch OFF or disconnect the power cord.

3. Remove the pump front cover by loosening the four cover screws (two on each side of the pump).

4. Using a18 Allen wrench, loosen the lock screw located on the front of the cylinder mounting block.

5. Disconnect the transducer cable from the pump and remove the inlet and outlet tubing.

6. Unscrew the cylinder from the cylinder housing.

Note

It may be necessary to use a tubing strap wrench or the Isco wrench package to unscrew the cylinder without marring its outer surface.

7. Lift the cylinder off the piston and push rod.

2-16

Installation 1. Install the O-rings in the grooves of the cooling jacket.

2. Lubricate the O-rings with soapy water or a light oil to ease assembly of the cooling jacket onto the cylinder.

3. Slide the cooling jacket onto the cylinder using a twisting motion.

Note

It is important that the cooling jacket be installed with the locking set screw away from the cylinder cap.

D Series Syringe Pumps

Section 2 Fluid System Connections and Accessories

CAUTION

Be careful not to damage the O-rings when pushing them over the threads of the cylinder.

Cylinder cap

O-ring

Outlet connector

Pressure transducer cable

Temperature control jacket assembly

Outlet tubing

(not supplied)

port

Cooling Jacket lock 10-32 set screw

Cylinder housing

Figure 2-16 Temperature control jacket installation

Reassembling the pump Refer to Figure 2-16 for the following steps.

1. Lower the cylinder/cooling jacket assembly over the piston and push rod assembly.

Cylinder lock 1/4-20 set screw

Cylinder

Inlet connector

port

Inlet tubing

(not supplied)

O-ring

Front cover

Cover screw

(4X)

2-17

D Series Syringe Pumps Section 2 Fluid System Connections and Accessories

Note

To avoid seal damage, ensure that the cylinder is aligned straight with the piston before lowering.

2. Screw the assembly into the cylinder housing until the cylinder is snug against the piston and no longer turns.

3. Unscrew the cylinder a minimum of

4. Line the inlet and outlet cylinder cap ports up as before. Turn the cooling jacket ports to the desired location and lock the cooling jacket to the cylinder by tightening the set screw.

5. Lock the cylinder by tightening the lock screw.

6. Replace the front cover and adjust both covers so they are flush with the cylinder housing.

7. Reinstall tubing.

8. Reconnect the pump pressure transducer cable.

2 a turn.

2.4.3 Back Pressure Regulation

The back pressure regulator option from Teledyne Isco reduces flow noise and improves pump performance at pressures less than 3.5 bar. Two back pressure regulators are available:

• 7 bar, part#209-9012-22

• 5 bar, part#209-9012-21

Fittings to connect the tubing to the regulators are supplied.

DANGER

RISK OF INJURY. THIS EQUIPMENT PRODUCES HAZARDOUS PRESSURES. PLEASE UTILIZE APPROPRIATE TUBING AND CONNECTIONS AS NOTED INTHE MANUAL.

Installation 1. Note the arrow on the regulator indicating the fluid

direction.

2. Connect your tubing between the pump outlet and the regulator using the supplied fittings.

Figure 2-17 Back pressure regulator

2-18

D Series Syringe Pumps

Section 2 Fluid System Connections and Accessories

2.5 Seal Options The pump contains four seals and a wear ring.

Transducer seal – The transducer seal is gold-plated to press into minute gaps between the surfaces of the transducer flange and the shelf of the cylinder cap.

Note

The 30D and 65D do not have transducer seals.

Cylinder cap seal – The cylinder cap seal maintains the pressure within the cylinder.

Piston seal – The piston seal maintains the pressure within the cylinder.

Wear ring – The wear ring supports the seal against movement within the cylinder and keeps the piston centered in the cylinder bore.

Wiper seal – The wiper seal cleans the inside of the cylinder. In the model 1000D pump, the wiper seal faces the same direction as the piston seal to prevent leakage of the wash gland fluid.

Figure 2-18 Inside the pump cylinder: wetted materials

The standard seals for each pump model satisfy the requirements of most applications, including those that use carbon dioxide, such as SFE. However, certain solvents and/or conditions require special cylinder seals to facilitate the application. Table 2-7 on the following page is provided to help you select the correct seal for your pump and application requirements.

Piston seal

retainer

Pressure

transducer

Transducer

seal

Cylinder cap

seal

Piston seal

Wear ring

Wiper seal

2-19

D Series Syringe Pumps Section 2 Fluid System Connections and Accessories

Table 2-7 Seal Selection Chart

Seal Type Max

GENERAL/SFE CARBON DIOXIDE

HIGH

TEMPERATURE

LOW PRESSURE

AQUEOUS 0-689.5

Pressure

0-689.5

bar

This is our standard seal. It is good for most applications, particularly those using organic solvents,

such as: LC, SFC, and SFE.

0-689.5

bar

This seal is best suited for applications which require temperatures between 50°C and 100°C. Its

chemical compatibility is similar to that of the general seal.

0-137.9

bar

This seal may provide better sealing at lower pressure. Its chemical compatibility is similar to that of

the general seal.

bar

This seal has better wetting properties, making it a good choice for aqueous solutions.

It is also the best choice for electrochemical detection.

Note: This seal requires a special break-in procedure before installation. See Section 9.9.2.

Seal

Description

Black, PTFE graphite filled

Black, PTFE graphite fiber reinforced high temperature compound

Black, PTFE graphite filled single point contact

White-translucent, ultra-high molecular weight polyethylene

65D/DM 100DX/DM 260D 500D/

202-9096-08 202-9090-75 202-9091-06 202-9091-56 upper seal

500HPX

1000D

202-9990-25 lower seal 202-9990-23

202-9090-76 202-9091-09 202-9093-56 upper seal

202-9990-28 lower seal 202-9990-27

202-9092-75 202-9092-06 202-9092-56 N/A

202-9090-77 202-9094-06 202-9094-56 N/A

AMMONIA (NH3) NITRIC ACID

0-275.8

bar

This is the only seal recommended for ammonia.

White, virgin PTFE 202-9090-78 202-9091-07 202-9091-57 upper seal

Note

Call the factory for seal availability for the 30D pump.

202-9990-26 lower seal 202-9990-24

2-20

D Series Syringe Pumps

Section 3 Basic Programming and Operation

3.1 Introduction This section will familiarize you with the D Series pump con-

troller and describe operating the pump under each of the various modes: constant flow, constant pressure, dispense, and refill.

Pump setup and operation is regulated by the D Series controller. Operating parameters are entered via the keypad on the front panel of the controller. Operating selections are displayed as menu items on the controller screen or are associated with a dedicated key on the controller keypad. Operating modes such as CONST FLOW, CONST PRESS, DISP, and REFILL all have such dedicated keys.

WARNING

UL (Underwriter Laboratories) has certified the D Series Controller and Pumps on the basis that explosive chemicals or chemicals thatcould becomeexplosive under pressure are NOT used. The instruments are not explosion proof. Use extreme caution when pumping hazardous fluids.

3.2 General Controller Information

3.2.1 Rates, Units, and Limits

3.2.2 Programming Screens The programming screens are divided into separate menus.

The following information is intended to familiarize you with controller operation. Once you have become familiar with the keypad and the main menu, you will find it easy to direct the pumping operations required for your applications.

If you make an incorrect entry, press CLEAR ENTRY to delete your last keystroke. If you have entered a programming mode but do not wish to make any changes, press ENTER to keep the current setting, or press softkey D to return to the previous screen.

To allow pump operation to be tailored to your application, both the pressure and flow rate units may be set by the user, refer to Section 3.4.1.

The pump also allows user programmed refill, as well as pumping rates, refer to Section 3.4.3.

The system protection limits may also be set by the user, refer to Section 3.8.7.

These menus are accessed when different features are being programmed. For complete information about programming menus, refer to Sections 3.3 through 3.7.

3-1

D Series Syringe Pumps Section 3 Basic Programming and Operation

3.2.3 Run Screens The run screen appears once a program has been loaded and the

pump is running. The run screen, which is determined by your program selections,

displays current information about pump operation. The following sections explain the display line by line.

Line One – No matter what mode you are in, the first line of the run screen is always the same.

Active Pump

PGa

Actual Pressure

.000 mL/MIN

Current Mode

Volume Remaining

PSI

Actual Flow Rate

XXX.XX ml

Line Two – The second line varies slightly depending on the operating mode. A file or step will only be displayed when operating in the gradient mode.

Elapsed Time

FILE/STEPSTOPPED

Status

Step of program running or loaded file number

000:00:00

Line Three – Line three varies depending on the mode; the rate and units are set by the user, so these will vary depending on your programming requirements. The ending time will always be displayed on this line.

3-2

Ending Time

Current Pressure

PS/MIN

FINAL:

Rate

PSI

Final Pressure

00:00:00

Line Four – Line four varies depending on the mode. The options presented on this line are softkey selectable, i.e. you use the softkeys (A - D) located under the screen to choose the option.

D Series Syringe Pumps

Section 3 Basic Programming and Operation

3.2.4 Selecting Operating Parameters

There are four ways to program the system from a menu screen. Menu Selection – The number keys are used to select from

listed menu items. Softkey Selection – The softkey programming options appear

on the fourth line of the screen. To either toggle or select an option, press the softkey, A-D, directly below that option.

Keypad Selection – The programming keys are located on the left side of the keypad. The pump mode, limits, and refill are all options that may be selected from the keypad.

Value Selection – The number keys are used to enter all numeric values required for pump operation. When a numeric value is required, a message will blink, prompting you to enter an appropriate value.

3.3 Main Menus There are four main menu screens. Access Menu 1 by pressing

MENU. To move forward and back between Menus 1, 2, 3 and 4, or

to return to the run screen, use softkeys A (MORE) and D (PRE-

VIOUS/RETURN).

Use the number keys to select a menu option. Selecting a menu option displays the programming parameters for that option in menu form.

Note

To exit a menu when multiple pumps are connected, press the

STORE,RECALL,CLEAR ENTRY OR MENU key.

3.4 Menu One Menu 1 provides programming options for units of measure,

pump selection, refill settings, power failure response, system reset, and display contrast adjustment.

To save all settings and return to the main menu, press RETURN

(D).

Figure 3-1 Menu 1 program selections

3.4.1 Flow and/or Pressure

Displayed units are user-selectable by selecting menu 1 item 1.

Units of Measure

Figure 3-2 Units menu

1. UNITS

2. SELECT PUMP

3. REFILL

PRESSURE UNITS=___________FLOW UNITS=__________

4. POWER FAILURE [STOP]

5. SYSTEM RESET

6. DISPLAY CONTRAST

AT M BAR

PSI

kPA

RETURN

mL/MIN

mL/HR

µL/MIN µL/HR

8. PREVIOUS

3-3

D Series Syringe Pumps Section 3 Basic Programming and Operation

Use numbers 1-4 to select the pressure units (ATM, BAR, PSI, kPa). The units selected will be displayed on the first line after PRESSURE UNITS=.

Use numbers 5-8 (mL/MIN, mL/HR, µL/MIN, µL/HR) to set the flow rate units. The selected units will be displayed on the first line after FLOW UNITS=.

3.4.2 Pump Selection (if multiple pumps)

If multiple pumps are connected to a single controller, the SELECT

PUMP option selects the pump (A, B, C, or D) whose current state

and settings appear on the display. The letter of the current pump will appear in lower-case in the

upper left corner of the run screen.

Note

When multiple pumps are connected, the UNITS selected for one pump will apply to all of the pumps.

3.4.3 Refill The refill option allows you to set the refill rate or set the pump

to automatically refill when a certain volume is reached. Press MENU > REFILL (3). The auto refill menu will appear.

Note

If more than one pump is connected to the controller, pump B, pump C, and pump D will be displayed on the fourth line. To select a pump, press the softkey under the pump designation.

To set auto refill volume Press 1 to set the volume for pump A.

The units to the right of the symbol will blink, indicating that you should enter a volume. Use the number keys to enter an appropriate value and then press ENTER.

To set refill rate 1. Press 3 to set the refill rate for the designated pump. The

refill rate can also be changed from the main screen while the pump is refilling.

2. A message will blink on the screen prompting you to enter the selected refill rate.

3. Enter the desired rate with the number keys; press ENTER.

4. To save and exit the refill menu, press D, PREVIOUS.

Disable/Enable auto refill The first line will display “OFF” or “ON”, indicating whether or

not this feature is enabled for pump A (or the currently selected pump). Press 4 to toggle this feature off or on for each pump.

3-4

Note

Auto refill can be set independently for each pump.

If AUTO REFILL is ON, the pump will automatically switch to refill mode when the volume reaches the auto refill mark. After refilling, pumping will resume in the programmed mode. The ACCESSORY outputs, which drive powered valves, will switch in sequence.

D Series Syringe Pumps

Section 3 Basic Programming and Operation

Unless otherwise specified, the pump will refill to full cylinder capacity. To specify a smaller refill volume, press 2 and enter the desired volume. Press 5 to toggle this feature off or on for each pump.

Note

The system can also be programmed to refill a pump based on an external analog input voltage, with a range of 0 to 11.5 volts. Information about this feature is provided in Section 3.12.3.

3.4.4 Power Failure [STOP] This feature allows you to set the activity of the pump in the event of a power failure. Press

4 to toggle this feature between

[STOP] (to remain stopped after power is restored) or [CONT] (to automatically resume after power is restored).

Note

In a multiple-pump system, this action is applied to all connected pumps.

3.4.5 System Reset (Restore

Default Settings)

To restore default program settings, press 5. To continue with the

reset, press

CONTINUE (A); to cancel the reset, press DO_NOT (D).

CAUTION

Resetting the system erases all programs and user settings.

This is a basic reset. For information about performing a “hard” reset, refer to Section 9.4.2.

3.4.6 Display Contrast From the menu, you can adjust the screen brightness for your light conditions and viewing angle.

Use softkeys B, DOWN, or C, UP to reduce or increase the brightness.

3.5 MenuTwo Menu 2 provides programming options for serial communication,

pump status, external control, multiple pump operation, volume reset, and valve control.

To save all settings and return to the main menu, press PRE-

VIOUS

/RETURN (D).

1. SERIAL OPTION

2. STATUS

3. EXTERNAL

4. MULTI PUMP

5. TOTAL VOL RESET

6. VALVE

Figure 3-3 Menu 2 program selections

3-5

D Series Syringe Pumps Section 3 Basic Programming and Operation

3.5.1 Serial Option The serial option menu allows you to set the baud rate and the unit identification number.

BAUD RATE = 1200 UNIT # ID = 6

NEXT BAUD

NEXT ID#

Figure 3-4 Serial option menu

Use softkey A, NEXT BAUD, to scroll through the available baud rates. These are: 300, 1200, 2400, 4800, 9600, 19.2K, 38.4K,

57.6K, and 115.2K. Use softkey B, NEXT ID#, to scroll through the available ID

numbers. These are 1-7. Six is the default, as this is the unit identifier for the Teledyne Isco LabView™ software.

For a complete discussion of serial control, refer to Section 8 in this manual.

3.5.2 Pump Status This option displays the controller software revision, and model of connected pump(s).

The first line displays the revision of the software. Lines 2, 3, and 4 display the type of pump connected to the A, B,

C, and D pump connectors, respectively. This screen is also momentarily displayed automatically each

time the pump controller is switched on.

3.5.3 External Control The pump pressure or flow rate operation can be controlled externally with an analog voltage. Complete instructions for this feature are provided in Sections 3.12 through 3.12.5.

3.5.4 Multiple Pumps One controller can control up to four pumps at once, either together or independent of each other.

Complete information about using the MULTI PUMP feature is provided in Section 3.9 and Section 5.6.

3.5.5 Total Volume Reset This option resets the total volume display of multi-pump pair AB or multi-pump pair CD to zero when operating in continuous flow or modifier mode.

1. AB PUMPS

2. CD PUMPS

3. AB AND CD PUMPS

Figure 3-5 Total volume reset

3-6

D Series Syringe Pumps

Section 3 Basic Programming and Operation

3.5.6 Valve This feature identifies the type of valves (passive, active (air), active ball (air), or electric) being used for the flow operation. Valves are set to “active” by default. If “active valve,” “active ball valve,” or “electric” is selected, the controller will match the pressure more closely before switching delivery pumps.

If “active ball” valve is selected, the controller will have a timed delay after valve open/closure before switching delivery pumps. This delay is to accommodate the slower opening/closing ball valves.

The number for the selected valve type will be blinking. Press 1, 2, 3, or 4 to select the correct valve type.

Note

The 30D pump has manual valve kits part number #60-1247-180 and #60-1247-181.

Electric Vale

Check Valve

Air Valve

Ball Valve

Figure 3-6 Types of valves

3-7

D Series Syringe Pumps Section 3 Basic Programming and Operation

3.6 MenuThree Menu 3 provides programming options for poor fills, diagnostics,

pressure calibration, alternative pressure inputs, pressure control setting, and pressure setpoint tolerance.

To save all settings and return to the main menu, press PRE-

VIOUS

/RETURN (D).

1. POOR FILL ALARM

2. DIAGNOSTIC MENU

3. PRESS. CALIBRATION

4. DIFF. MODES

5. PRESS. INTEGRAL

6. PRESS. DEADBAND

Figure 3-7 Menu 3 program selections

3.6.1 Poor Fill Alarm In constant pressure mode, this feature allows you to set a fill point as a percentage of pump volume. If this volume percentage is not reached after a refill and re-pressurization, the system issues an alarm and stops the pump.

3.6.2 Diagnostic Menu This selection displays an additional menu containing testing options for the system. Refer to Section 9.5 for a complete explanation of how to use the diagnostic menu.

3.6.3 Pressure Calibration This is a stored psi value to ensure optimal operation of the pressure transducer. On most pumps, this value, known as the “midpoint adjustment,” can be found on a tag on the transducer cable (shown below).

Note

The midpoint adjustment value is individual to each pump, and is stored within the controller software. Therefore, this value must be re-entered if the controller or pump is switched, controller is reset, or if a pump is plugged into a different port on the controller.This calibration feature is not applicable to the HPx or 30D pump models.

Standard calibration for the transducer is a two-point adjustment, at zero and maximum pressure. The midpoint adjustment number is the difference between the pressure at half maximum, as read by a pressure gauge with a precision of

0.25% accuracy, and the syringe pump transducer output.

Figure 3-8 Location of transducer midpoint value

3-8

D Series Syringe Pumps

Section 3 Basic Programming and Operation

Press CALA, CALB, CALC, or CALD to select the correct pump, enter the calibration number, and press ENTER.

3.6.4 Diff. Modes This feature allows the use of other analog inputs for pressure input to the pump. The standard pressure transducer MUST be connected to pump in order for this feature to work.

Press 1, OFF, to turn off this feature. Press 2, 0 to 50 ANLG1, to use a 5 volt 50 psi transducer on ANALOG INPUT 1 of the ACCESSORY connector. Press 3, Custom ANLG1, to use 5 volt custom pressure transducer on ANALOG INPUT 1 of the ACCESSORY connector. Press 4, 500 ANLG2, to use a 5 volt 500 psi pressure transducer on ANALOG INPUT 2 of the ACCESSORY connector. Press 5, 5000 ANLG3, to use a 5 volt 5000 psi transducer on ANALOG INPUT 3 of the ACCESSORY connector.

3.6.5 Press. Integral The pressure control algorithm can be adjusted by turning OFF the integral compensation. This may be desirable at very low flow rates or during static tests. Under these conditions pressure control may be more stable with integral compensation off. If the pressure integral compensation is off, there will be a pressure error proportional to the delivery flow rate. For this reason, the pressure integral is normally turned on for anything but very low flow rates.

This feature is ON by default. Press 1, 2, 3, or 4 for the associated pump A, B, C or D to toggle the feature OFF.

3.6.6 Pressure Deadband This feature prevents pump pressure “hunting” when delivering at low flow rates (<0.12 ml/min for the 30D pump or < 0.25 ml/min for other models). When this function is ON, the pump pressure may exceed the set point by up to 3 psi without the piston backing up to reduce the pressure. If the pump pressure exceeds the set point by more than 3 psi, the motor will reverse the piston until the 3 psi dead band maximum is reached. No dead band is allowed if the flow rate is above 0.25 ml/min (0.12ml/min for 30D pump) or when the pressure error is negative (pressure below the set point).

If the 3 psi positive pressure error is not acceptable at low flow rate, toggle the dead band feature OFF by pressing 1, 2, 3, or 4 for the associated pump A, B, C or D. This feature is ON by default.

3.7 Menu Four

3.7.1 Modbus Options Beginning with Rev. 1.05 firmware, the Modbus RTU communi-

cations protocol is supported. Rev. 2.0 or higher firmware supports Modbus TCP communications protocol. Complete information about using Modbus RTU and Modbus TCP communication is provided in Section 7 Modbus Configuration.

3-9

D Series Syringe Pumps Section 3 Basic Programming and Operation

3.8 Front Panel Keys In addition to the menu options, certain functions and modes are

selectable from the front panel keyboard.

3.8.1 CONST PRESS and

CONST FLOW

3.8.2 PRGM GRAD This key is used to place the pump system in gradient mode. For

3.8.3 HOLD The HOLD key is used while a gradient is running. When HOLD

3.8.4 RECALL The RECALL key can only be used when the pump(s) are stopped

These keys place the system in constant pressure mode or constant flow mode, respectively. Information for these operating modes can be found in Section 3.10.

complete information about gradient programming, refer to Section 4 Gradient Pumping for Pressure, Flow, and Concen- tration Modes.

is pressed, the program clock freezes and current gradient parameters are maintained.

To continue the gradient, press HOLD or RUN.

or when in HOLD mode. The RECALL key is used to recall a previously programmed gradient. When you select this option, the controller automatically loads the gradient and switches the pump to program gradient mode.

1. Press RECALL and use the number keys to enter the number name of the gradient you wish to recall. Press ENTER.

2. If you enter a number of a gradient that does not exist, the controller briefly displays the message “FILE NUMBER DOES NOT EXIST.” It then assumes you will be creating a new gradient under that number and displays the program gradient run screen.

3.8.5 STORE The STORE key is operational in the programmed gradient mode. It is used to save the program gradient parameters and exit the programming (parameter entry) mode.

3.8.6 REFILL The REFILL key manually places the pump in refill mode.

3.8.7 LIMITS The controller allows the user to set the minimum and maximum

flow rate limits, the minimum and maximum pressure limits, and the maximum rate the pump will run while controlling the pressure in constant pressure mode.

• When using a single controller to operate multiple pumps, you need to select the appropriate pump before setting any pump parameters. The available pumps will be displayed above the softkeys. These selections correspond with the connector that the pump control cable is plugged into on the rear panel of the pump controller. To select a pump, press the softkey under the pump designation. The top line of the screen will indicate the currently selected pump.

• The maximum and minium limits you set cannot exceed the pump specifications.

3-10

D Series Syringe Pumps

Section 3 Basic Programming and Operation

To set the limits 1. Press LIMITS. To display the Limits menu, enter the num-

ber of the limit you wish to program. One of five limit setpoint menus will appear: MAX PRESS, MIN PRESS, MAX FLOW, MIN FLOW, or FLOW LIMIT. The MAX PRESS limit setpoint menu is shown in Figure 3-10.

Figure 3-9 Limits menu

Figure 3-10 Limits setpoint (Max Press) menu

Note

These features are discussed later in this section under Limits Programming Options.

2. Press the number 1 key to set the value. A message will blink on the right side of the screen, prompting you to enter the selected limit.

3. Enter the desired limit setpoint, using the number keys.

4. Press ENTER to save the value.

LIMITS: CURRENT PUMP

MAX PRESS _____

MIN PRESS______ PUMP A

MAX PRESS_____PSI

SET PRESS VALUE

ON OFF

ALARM*DISPLAY*

MAX FLOW

MIN FLOW

FLOW LIMIT

SHUTDOWN*

____

_____

PREVIOUS*

Limits programming options

Note

The pump can be set to shut off or not at this limit condition by pressing softkey C under shutdown. This will toggle this option to ON.

5. To exit the limit menu, press the softkey D, PREVIOUS. If four pumps are connected press STORE, RECALL, or CLEAR ENTRY to exit the limits menu.

6. Once all the limits have been set, press softkey D, PREVIOUS, to return to the main menu.

In addition to setting the upper and lower limits, the pump also allows the user to specify whether they want:

• the alarm message displayed

• an alarm to sound when the limit is reached

• the pump to shut down when the limit is reached

3-11

D Series Syringe Pumps Section 3 Basic Programming and Operation

These features are set using the softkeys A - C, which toggle the feature on and off.

Note

The Max Press (maximum pressure) display on and alarm on options cannot be disabled.

Display

When a limit has been exceeded, this feature causes the display to automatically flash an OVER or UNDER LIMIT message. To turn off this function, press softkey A once to toggle to:

Alarm

When a limit has been exceeded, this feature causes the pump to automatically beep a warning. To turn off this function, press softkey B once to toggle to:

Shutdown

When a limit has been exceeded, this feature causes the pump to shut down. To turn off this function, press softkey C once to toggle to:

Flow rate limit for pressure control

When the pump is controlling pressure (CONSTANT PRESSURE MODE), the flow rate is not user-controlled, and may range up to the maximum flow of the pump. In some cases, it is desired to limit the rate of pumping during system pressurization. This can be done by selecting limit 5, FLOW LIMIT. The FLOW LIMIT value is used as the upper range of flow rate during pressure control. This limit is not the same as the MAX FLOW limit, which is a threshold above when the pump is stopped, or an alarm is activated as selected by the operator.

To save your changes and return to the main menu, press softkey D, PREVIOUS.

OFF

DISPLAY

OFF

ALARM

SHUTDOWN

3.8.8 RAPID PRESS This option is available when operating in the constant flow mode and in the two-pump concentration gradient mode. It allows rapid pressurization to a stable pressure point and then switches automatically to the constant flow setpoint. This is helpful when you are operating at a low flow rate but wish to rapidly pressurize a solvent.

1. Press CONST FLOW to put the pump in constant flow mode, or enter a two-pump concentration gradient.

2. Press RAPID PRESS.

3-12

D Series Syringe Pumps

Section 3 Basic Programming and Operation

3. The controller will display maximum flow rate and target pressure setting. If these values are correct, press D to continue rapid pressurization.

4. If you know approximately what the pressure will be when the system is stable, enter this value as a target pressure. Press A and enter the desired pressure value. This should shorten the time required to stabilize the system pressure.

5. If you would like to limit the maximum flow rate during the rapid pressurization phase, press B and enter the desired flow rate limit.

6. Press D to continue rapid pressurization.

3.8.9 DISP The DISP key activates Dispense Mode, for applications such as reactant feed and batch delivery, where a specified volume is pumped. Refer to Section 3.10.3 for programming steps.

3.8.10 ACC CTRL The ACC CONTROL key will allow you to manually operate accessories, such as valves, via the Digital Output terminals on the back of the controller.

1. Press ACC CTRL.

2. From the accessory control menu, use the number keys 1-8 (1-A INLET, 2-A OUTLET, 3-B INLET, 4-B OUTLET, 5-C INLET, 6-C OUTLET, 7- D INLET, 8- D OUTLET) to toggle the desired valve open or closed. (Numbers 1-8 represent digital output terminals 1-8, respectively).

3. To exit, press PREVIOUS (D).

3.8.11 ZERO PRESS The ZERO PRESSURE key will correct pressure sensor drift. Before pressing ZERO PRESS, the pump should have port fittings installed, and be depressurized.

1. Press ZERO PRESS. The display will show the current pressure and ask if you want to zero the pressure.

2. Press A, B, C, or D to zero the desired pump.

or,

If the pump is not depressurized, press DO_NOT (D) to exit the zero pressure operation. If four pumps are connected press the STORE,RECALL OR CLEAR ENTRY key.

3.9 Control of Multiple Pumps

When using multiple pumps, there are four multi-pump operating modes of delivery and one independent mode:

• Continuous flow in constant flow mode.

• Continuous flow in constant pressure mode.

• Modifier addition in constant pressure mode.

• Modifier addition in continuous flow, constant pressure mode.

• Independent mode.

3-13

D Series Syringe Pumps Section 3 Basic Programming and Operation

Note

The 30D pump cannot be used in multiple pump modes. Please contact the factory for details.

3.9.1 Multi-Pump Operation A D Series continuous flow pumping system in constant flow mode will consist of two syringe pumps and a valve package, all regulated by one controller. Installation and operating instructions for this system are provided in Section 5.

A pump modifier system includes two D Series syringe pumps, regulated by one controller and outlet check valves to prevent system back flow into the pump. To connect the system, you will need a modifier addition kit. Consult with the factory when configuring 65D pumps as a modifier system.Installation and operating instructions for this system are provided in Section 6.

In these modes, the softkeys toggle between the options described in Table 3-1.

Table 3-1 Key functions in the Multi-pump Mode

Key Display Option Description

A NORMAL Uses a finer (slower) pres-

FAST Uses a coarser (faster)

B NORMAL PRESS Uses pressure matching

LOW PRESS Uses no pressure matching

C DELIVER Sets the pump into the

RECEIVE Sets the pump into the

6 MIN/MAX POINTS Sets the fill and refill marks

sure match control when switching from one pump to the other.

pressure match control when switching from one pump to the other.

when switching from one pump to the other

when switching from one pump to the other.

delivery mode of operation.

receive mode of operation.

that are used with both continuous flow modes.

Sets the NCV feature On/Off. See Section 5.6.4.

3.9.2 Independent Control

of up to Four Separate Pumps

3-14

A D Series syringe pump controller can run four syringe pumps independently of each other in either constant pressure, constant flow mode, dispense mode, or any combination of the three. To set up this option, use the following procedure.

1. Press MENU.

D Series Syringe Pumps

Section 3 Basic Programming and Operation

2. Press softkey A, MORE.

3. Press number 4, MULTI PUMP. The multi-pump menu will appear.

4. Press number 1 for pumps A or B, press number 2 for pumps C or D.

5. Press number 4, INDEPENDENT. The controller will set the pumps to Independent mode. Number 4 will blink, indicating that INDEPENDENT mode is selected.

6. Select the HOLD PRESS or NORMAL mode of operation. Press softkey A to toggle between the two modes.

HOLD PRESS: In constant pressure mode, after the pump is empty, if the outlet pressure rises past the set point the pump will restart and run the system to the set point pressure.

NORMAL: This feature shuts the system down if a pump runs empty in constant pressure mode.

Once the pumps have been set to this mode, they will operate independently from one another. Each pump will operate at its defined limit and rate. Independent mode is the default setting for the pump.

When you select a command such as STOP or REFILL, the display will prompt you to designate which pump to stop or refill. Only the designated pump will stop; the other pumps will continue to run.

Note

In an emergency situation, toggling the On/Standby switch will cause all pumps to immediately stop.

7. Return to the run screen by pressing D three times. Then press D (SELECT PUMP). The display will show each pump’s information and allow you to select any pump for programming changes.

3.10 Operating Modes The pump has three delivery modes and one refill mode.

Note

When using a single controller to operate multiple pumps independently, you need to select the appropriate pump run screen before selecting a mode. To select the appropriate pump, press SELECT PUMP (D) and an intermediate screen will be shown. Press the softkey for the appropriate pump, the run screen for that pump will appear.

Constant Flow – Refer to Section 3.10.1 This mode is used when the flow rate must remain constant

during the pumping operation. Constant Pressure – Refer to Section 3.10.2

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D Series Syringe Pumps Section 3 Basic Programming and Operation

The constant pressure mode is used when the application of fixed pressure throughout the pumping operation is required. The pump will maintain the desired pressure by positive or negative displacement of the piston.

Programmed Gradient – Refer to Section 4 In the programmed gradient mode, the pump can provide the fol-

lowing types of gradient:

• Two-pump concentration gradient on pumps A and B

• Single-pump linear pressure gradients on pump A

• Single-pump flow programs on pump A

Dispense – For applications requiring delivery of a specific volume. Refer to Section 3.10.3.

Refill – Refer to Section 3.4.3. You can set the refill rate and change it when in refill mode.

3.10.1 Constant Flow To set constant flow operation, use the following procedure:

1. Press CONST FLOW. “CFa” will be displayed in the upper left corner of the screen. This denotes that you will be defining constant flow parameters for pump A. If you wish to define parameters for pump B, C, or D, press softkey D, select pump, and then press softkey A, B, C, or D to select pump A, B, C, or D respectively.

Note

If the main menu is displayed, you must press D under CONST FLOW.

2. Press A to change the flow rate. The words ENTER FLOW RATE will flash on the screen.

3. Use the number keys to enter the desired flow rate.

Note

If you make an error, press CLEAR ENTRY to delete one character at a time.

4. Press ENTER once the desired flow rate is displayed.

5. Press RUN to begin pump operation.

3.10.2 Constant Pressure Programming a constant pressure operation only requires a few keystrokes. Use the following procedure:

1. Press CONST PRESS; CPa will be displayed in the upper left corner of the screen. This denotes that you will be defining constant pressure parameters for pump A. If you

3-16

D Series Syringe Pumps

Section 3 Basic Programming and Operation

wish to define parameters for pump B, C, or D press softkey D, select pump, and then press softkey A, B, C, or D to select pump A, B, C, or D respectively.

Note

If the main menu is displayed, you must press softkey D under RETURN before pressing CONST PRESS.

2. Press the A softkey to indicate to the program that you wish to enter the pressure. The words “ENTER PRESSURE” will flash on the screen.

3. Use the number keys to enter the desired pressure.

Note

If you make an error, press CLEAR ENTRY to delete one character at a time.

4. Press ENTER once the desired pressure is displayed.

5. Press RUN to initiate pump operation.

3.10.3 Dispense Mode For applications such as reactant feed and batch delivery where a specified volume is pumped, Dispense Mode is available with Teledyne Isco syringe pumps.

Dispense mode flow rate begins at zero, ramps up to level out at the programmed flow rate, then ramps back down to zero, delivering a precise specified volume. The slope rate and run time are dependent upon the pump model being used.

Start

ramp up

Specified Flow

ramp down

Stop

Figure 3-11 Depiction of dispense mode sequence

Note

For time-controlled delivery, you must run the pump in standard gradient mode, as described in Section 4.

In order to access Dispense Mode control, the pump must be in Constant Flow mode. Press CONST FLOW to enter this mode. Toggle Dispense Mode off or on by pressing DISP. Note that pressing the DISP key while in Constant Pressure mode has no effect.

To adjust the flow rate, press FLOWRATE (A), enter the desired rate using the number keys, and press ENTER.

3-17

D Series Syringe Pumps Section 3 Basic Programming and Operation

To specify the volume of the batch delivered, press VOLUME (C), enter the desired volume using the number keys, and press

ENTER.

To dispense the batch, press RUN. The sequence runs once, then stops. To repeat the sequence, press RUN again.

Note

When the system is in Dispense Mode, all gradient modes, constant pressure mode, and auto-refill, as well as serial control, are unavailable until Dispense Mode is turned off.

3.11 External Control The pump can be externally controlled for pressure or flow rate

operation with an analog voltage or through the serial interface. The serial interface allows you to control the pump operation

from an IBM-PC or compatible computer that has an RS-232-C serial output. The serial interface accepts English command words from the computer, like constant pressure, refill, etc. For more information see Section 8 Serial Interface.

3.12 External Control: Analog

The syringe pump can be controlled externally by analog voltage in either constant flow or constant pressure mode. The input rangeis0–11.5volts (for all pumps), with a resolution of 5000 increments per volt.

Note

The input range for the 4-20ma option is set to 4-20ma

3.12.1 Wire connections Two wires are required for analog control. The analog common or ground wire should be connected to the GND terminal under ANALOG INPUT of the ACCESSORY connector on the controller rear panel. The analog control or input wire should be connected to terminal 1 under ANALOG INPUT.

If two pumps are used with the controller, the second analog control or input wire should be connected to terminal 2 under ANALOG INPUT. If three pumps are used with the controller, the third analog control or input wire should be connected to terminal 3 under ANALOG INPUT.

If four pumps are used with the controller, the fourth analog control or input wire should be connected to terminal 4 under ANALOG INPUT.

When using one of the multi-pump operation modes, only the ANALOG INPUT terminal 1 needs to be connected to control the A-B pump pair and ANALOG INPUT terminal 3 needs to be connected to control the C-D pair.

3-18

Note

For the 4-20ma input option the negative output wire connects to DIGITAL GROUND. The positive output wire connects to ANALOG INPUT 1-4.

D Series Syringe Pumps

Section 3 Basic Programming and Operation

3.12.2 Preparation Before programming the controller to accept the analog signal, you must know the maximum flow rate (constant flow) or the maximum pressure (constant pressure).

Note

The high and low limits you enter cannot exceed the pump specifications: Normal input = 10V.

Note

For the 4-20ma input option the negative output wire connects to DIGITAL GROUND. The positive output wire connects to ANALOG INPUT 1-4.

To determine the analog voltage range, use the formula explained below:



----

 Vmax=



where:

3.12.3 External Control for

Refill

FS = Maximum flow rate or Maximum pressure (Full Scale): This is the same value entered when setting LIMITS, as described in Section 3 of your user manual, for MAX PRESS or

MAX FLOW.

V = Volts per unit of flow or pressure U = Incremental unit of flow or pressure Vmax = Maximum input voltage

Note

For the 4-20ma input option The maximum flow or pressure sets the limit at 20ma. No other formula applies.

The refill option allows you to set the refill rate, or set the pump to automatically refill when a certain volume is reached. From Menu 2, the system can be programmed to automatically refill a pump based on an external analog signal, with a range of 0 to 11.5 volts.

To access this feature, press MENU > MORE (A) > EXTERNAL (3).

3-19

D Series Syringe Pumps Section 3 Basic Programming and Operation

FULL SCALE INPUT RANGE < XX.XXX VOLTS

OFFOFF

SET RANGE EXTERNAL REFILL

Figure 3-12 External control for refill menu

To set the full-scale value (maximum 11.5 volts), press SET RANGE (A), enter the desired value, and press ENTER.

To place the pump under external control, press EXTERNAL (B). The feature will turn from OFF to ON.

To SET the external control for refill, press REFILL (C). The feature will turn from OFF to ON.

3.12.4 Calculation Examples Flow Rate – If MAX FLOW is set at 25 ml/min in the LIMITS menu and the desired scale factor is 5.0 volts per 20 ml/min, do the following to determine the analog voltage range:



 6.25=

----- -



In this example, the analog voltage range would be 0.0–6.25 volts.

Pressure – If MAX PRESS is set at 510.2 ATM in the LIMITS menu and the desired scale factor is 2.0 volts per 100 ATM, do the following to determine the analog voltage range:

510.2

-------- -



100



 10.204=

In this example, the analog voltage range would be 0.0–10.204 volts.

Note

If the controller is equipped with 4-20ma input then the low signal is 4ma (stop) and high signal is 20ma (run).

3.12.5 Setup First, select the desired operating mode by pressing CONST PRESS or CONST FLOW.

3-20

CP or CF and selected

( pump are displayed)

D Series Syringe Pumps

Section 3 Basic Programming and Operation

(EXTERNAL)

(On/Off)

(MORE)

(

SET RANGE)

Enter Vmax value

Figure 3-13 Button sequence for external control setup

3.13 Remote RUN/STOP The D series syringe pump RUN/STOP function can be exter-

nally controlled by a switch contact closure or TTL input. The input voltage is 5 volts and is internally pulled high (RUN). The input is level sensitive (must remain high for RUN or low for STOP) and must be high for normal operation of serial (RS-232) control.

To enable the remote RUN/STOP feature, press MENU >MORE> (A) > EXTERNAL >(3)>DIGITAL (A) ON. Then press RUN or force the RUN/STOP pin low to enable the pump. Thereafter, the RUN/STOP pin will control operation.

first press RUN or force the RUN/STOP pin low to enable the pump. Thereafter, the RUN/STOP pin will control operation. Pressing STOP on the front panel will override the RUN/STOP pin.

3.13.1 Wire Connections Two wires are required for external RUN/STOP control. The digital common or ground wire should be connected to one of the four DIGITAL GROUND terminals of the ACCESSORY connector on the controller rear panel. The control wire should be connected to terminal 1, under DIGITAL INPUT. If an electrically isolated relay is used, one relay terminal should be connected to digital ground and the other to terminal 1, under DIGITAL INPUT.

3-21

D Series Syringe Pumps Section 3 Basic Programming and Operation

If two pumps are used with the controller, the second control wire should be connected to terminal 2, under DIGITAL INPUT.

If three pumps are used with the controller, the third control wire should be connected to terminal 3, under DIGITAL INPUT.

If four pumps are used with the controller, the fourth control wire should be connected to terminal 4, under DIGITAL INPUT.

Note

For the 4-20ma option, the negative current wire is connected to DIGITAL GROUND and the positive should be connected to DIGITAL INPUT

3.14 Analog Flow Rate and Volume Output

Options

3.14.1 Voltage Analog Output Output voltage for pressure is located on the back panel of the

This output can also be: 4-20 mA OUTPUT ANALOG OUTOUT ELECTRIC VALVES 12-CH OUTPUT

25-pin cable

The analog output option provides for analog monitoring of the syringe pump flow rate. If two or less pump modules are connected to the controller, pump volume delivered can also be monitored via analog output. These outputs are often used with analog based plant or process monitoring equipment.

pump module via standard banana jacks. The output connection is the female 25-pin Sub-D connector

located on the rear panel of the controller, under 4-20mA Output.

DIGITAL I/O

4-20mA OUTPUT

USB / ETHERNET

RS-232 / RS-485

Controller rear panel

Figure 3-14 Analog voltage output connection, rear panel

Voltage analog outputs are pre-installed at the factory. This option has a total of four outputs for monitoring flow rate with selectable ranges from 0 to 5V, -5 to +5V, and 0 to 10V. The default range at installation is 0 to 10V. Each output can be set to a different range.

The top of the selected range is the same maximum value entered for MAX FLOW when setting LIMITS, as described in Section 3.8.7 of this manual. For example: if the output range is

3-22

D Series Syringe Pumps

Section 3 Basic Programming and Operation

5V and the preferred output scale is 1 volt per 10 ml/min, MAX

FLOW is set at 50 ml/min. MAX FLOW cannot exceed the pump

specification. A controller running one to two pumps can also output cylinder

volume on the remaining outputs. The volume output range is not adjustable. Full scale is equal to one pump stroke.

Figure 3-15 Output range selection jumpers

Table 3-2 Analog Output Options (connections to

Pin No. Description One or Two

14 Output #1 Flow Rate A Flow Rate A

15 Output #2 Flow Rate B Flow Rate B

16 Output #3 Volume A Flow Rate C

17 Output #4 Volume B Volume B

For additional information about analog output options, download technical bulletin TB34Syringe Pump Analog Output Options.

female 25 pin Sub-D)

Pump Function

1 Chassis (earth) — —

2 Analog common — —

3 Analog common — —

4 Analog common — —

5 Analog common — —

Three Pump

Function

3.14.2 Current Loop Output The pump controller can be configured to provide a 4–20 mA current loop output. The 4-20mA analog output board is pre-installed at the factory, but can also be purchased in an upgrade kit. The board initially contains three output cards, for flow rate, pressure, and volume for Pump A. Additional cards can

3-23

D Series Syringe Pumps Section 3 Basic Programming and Operation

be purchased to output data for one or two more pumps. Refer to Table 3-3 for connections for the 4-20mA and 0-10v 12 channel outputs.

Note

Connections to the controller DB-25 are the same as shown in figure 3-14. Refer to Table 3-3 and Table 3-4 for cable connections to your equipment.

Table 3-3 Analog Output Signal/Sub-D

Pin # Channel Data Type/Pump

1 Pump A Flow Rate (Signal Return)

2 Pump A Pressure (Signal Return)

3 Pump A Volume Remaining (Signal Return)

4 Pump B Flow Rate (Signal Return)

5 Pump B Pressure (Signal Return)

6 Pump B Volume Remaining (Signal Return)

7 Pump C Flow Rate (Signal Return)

8 Pump C Pressure (Signal Return)

8 Pump C Volume Remaining (Signal Return) 23 10 Pump D Flow Rate (Signal Return) 24 11 Pump D Pressure (Signal Return) 25 12 Pump D Volume Remaining (Signal Return)

Pins for 4-20mA

Pump A Flow Rate

Pump A Pressure

Pump A Volume Remaining

Pump B Flow Rate

Pump B Pressure

Pump B Volume Remaining

Pump C Flow Rate

Pump C Pressure

Pump C Volume Remaining

Pump D Flow Rate

Pump D Pressure

Pump D Volume Remaining

3-24

D Series Syringe Pumps

Section 3 Basic Programming and Operation

Table 3-4 Analog Output Signal/Sub-D

Pins for 0-10 VDC

Pin # Channel Data Type/Pump

1 14 Pump A Flow Rate (Signal Return)

2 15 Pump A Pressure (Signal Return)

3 16 Pump A Volume Remaining (Signal Return)

4 17 Pump B Flow Rate (Signal Return)

5 18 Pump B Pressure (Signal Return)

6 19 Pump B Volume Remaining (Signal Return)

7 20 Pump C Flow Rate (Signal Return)

8 21 Pump C Pressure (Signal Return)

9 22 Pump C Volume Remaining (Signal Return) 10 23 Pump D Flow Rate (Signal Return) 11 24 Pump D Pressure (Signal Return) 12 25 Pump D Volume Remaining (Signal Return)

Pump A Flow Rate

Pump A Pressure

Pump A Volume Remaining

Pump B Flow Rate

Pump B Pressure

Pump B Volume Remaining

Pump C Flow Rate

Pump C Pressure

Pump C Volume Remaining

Pump D Flow Rate

Pump D Pressure

Pump D Volume Remaining

3-25

D Series Syringe Pumps Section 3 Basic Programming and Operation

3-26

D Series Syringe Pumps

Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes

4.1 Introduction Gradient pumping is used in applications requiring time-con-

trolled or rate-controlled delivery of a specific volume at a set flow rate or pressure, or a two-pump flow concentration.

All Teledyne Isco syringe pump models can be used in gradient mode. The model 65D requires special hardware for this. Call the factory for complete information.

You can program the controller to increase or decrease pressure or flow during different steps within a single program by entering a specific value at the beginning and end of each step.

DANGER

RISK OF INJURY. THE PRESSURE PRODUCED COULD BE 700 BAR. PLEASE UTILIZE APPROPRIATE TUBING AND CONNECTIONS NOTED IN THE MANUAL.

Tools and Parts for Single Pump System

Open-end wrenches:1/4",5/16",7/16",3/8" Manual Refill Valve Kit - see Table 4-1 Manual Outlet Valve Kit - see Table 4-2

Tools and Parts for Dual Pump System

Open-end wrenches:1/4",5/16",7/16",3/8" Manual Refill Valve Kit -

see Table 4-1 (two kits required) Gradient Mixer Package - part #68-1247-080

Table 4-1 Manual Refill Valve Kits

Pump Model Part Number

1000D 68-1247-117

500D 68-1247-083

65DM, 100DM/DX, 260D 68-1247-077

65D 68-1247-127

30D 60-1247-180

4-1

D Series Syringe Pumps Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes

Table 4-2 Manual Outlet Valve Kits

Pump Model Part Number

1000D 68-1247-118

500D 68-1247-082

65DM, 100DM/DX, 260D 68-1247-078

65D 68-1247-126

30D 60-1247-181

4.2 Connecting the System

The syringe pump has two ports at the top of the cylinder. One port is used as the inlet for filling the pump, and the other as the outlet (either port may be used as inlet or outlet). Inlet and outlet connections to each pump must be m ade identically. Standard plumbing connections vary between pump models. See Table 4-3 for standard port information.

When making fluid connections that use ferrules, be sure to use the ferrules supplied for that pump by Teledyne Isco. Push the tubing completely into the connector and finger-tighten. Then tighten with a wrench to clamp the ferrules onto the tubing.

Table 4-3 Swaging Detail

65DM, 100DM/D X, 260D

500D

1000D

/8" Valco

/8" NPT

/4" NPT

FERRULE

FRONT FERRULE

BACK FERRULE

FRONT FERRULE

BACK FERRULE

NUT 5-16 - 24

1/2 TURN PAST FINGER-TIGHT

NUT 5-16 - 24

3/4 TURN PAST FINGER-TIGHT

NUT 7-16 - 20

1-1/4 TURN PAST FINGER-TIGHT

4-2

65D/30D

/4" F250C

Note

Pump models 260D, 100DM/DX, and 65DM have a direct connection, as shown in Figures 4-1 and 4-2 on the following page. Valve kits for other models include male adapter fittings.

D Series Syringe Pumps

Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes

A gradient pumping system includes high-pressure, two-way valves that connect the pump inlets to fluid reservoirs, and the pump outlets to the gradient mixer (dual pump system) or other apparatus (single pump system). Each refill kit and outlet valve package contains one two-way valve; the gradient package contains two. The kits contain

Two-Way

Valve part

#209-0098-05

all tubing and hardware necessary for valve installation.

Following installation, the tubing connections must be tested for leaks before any program is run. If a leak is found, tighten the connection slightly. If the leak persists, swage the connection again with a new ferrule. Refer to Technical Bulletin TB05 Field

Verification Procedures at

http://www.isco.com/sp_applications/#techbulletins for leak test procedures.

4-3

D Series Syringe Pumps

Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes

4.2.1 Inlet Connections Kit components and connections are shown in Figure 4-1.

1. Mount the inlet valve on the pump housing with the spacer block and screws provided.

2. Connect the pre-bent SST tubing from one port of the valve to the pump inlet. Use the nut and ferrule to connect the tubing at the inlet and the valve fittings to connect the tubing at the valve.

3. Connect the PTFE refill tubing (with the filter) to the other port of the valve, using the nuts and ferrules supplied.

Note

Notes at http://www.isco.com/sp_applications/#techbulletins).

nut, ferrule

pre-bent

SST tubing

PTFE tubing

stainless steel tubing

filter

system

spacer bloc

valve

Figure 4-1 Pump inlet connections

4-4

Teledyne D series, 30D, 100DX, 65D, 260D Installation And Operation Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Foreword

Table of Contents

Section 1 Introduction

1.1 About This Manual The 65DM, 100DM, 100DX, 260D, and 500D pumps have

1.2 Specifications The technical specifications for the D Series Syringe Pumps are

1.3 Unpacking After removing the pump, controller, and accessories from the

1.4 Controls and Indicators

1.5 Electrical Connections The pump controller may be placed on top of the pump, or

1.5.1 Cabling For all systems, connect the control cable of the pump module (Pump A for multiple-pump systems) to the connector labeled PUMP A on the rear panel of the controller. Subsequent pumps in multiple-pump systems can be connected to controller connectors PUMP B, PUMP C, and PUMP D.

1.6 Preliminary Checkout After the electrical connections have been completed, follow this

Section 2 Fluid System Connections and Accessories

2.1 Introduction This section discusses general fluid system connections, and the

2.2 Fluid System Connections

2.2.1 Ports There are two ports in the pump standard cylinder cap. Either

2.2.2 Installation Tips • Be sure to keep the tubing as straight as possible at the end, as this will make it easier to install the ferrules.

2.2.3 Tubing Requirements The tubing must be cut squarely to prevent possible problems. Square ends are easier to insert through the ferrule, and will decrease dead volume.

2.2.4 Accessory Packages The accessory packages for all pumps contain fittings that allow you to attach tubing to the pump (refer to Figure 2-1), as well as appropriately sized cylinder seals. Note that the tubing is not included.

2.2.6 Draining Overflow The drip tray outlet on the pump cylinder provides a means of draining fluid from seal leakage. Use the14" ID flexible tubing included with the accessory package to divert the leakage away from the pump.

2.2.9 Flushing Pumps with External Transducers

2.3 Fluid Connection Accessories

2.3.1 Manual Refill Kit The optional manual refill kit provides a high pressure, two-way

2.3.2 Manual Outlet Valve Kit

2.3.3 CO2Cylinder Connection Package

2.3.4 In-Line Filter Package The optional in-line filter package should be used when it is

2.4 Temperature and Pressure Controls

2.4.1 Cylinder Insulating Cover

2.4.2 Temperature Control Jacket

2.4.3 Back Pressure Regulation

2.5 Seal Options The pump contains four seals and a wear ring.

Section 3 Basic Programming and Operation

3.1 Introduction This section will familiarize you with the D Series pump con-

3.2 General Controller Information

3.2.1 Rates, Units, and Limits

3.2.2 Programming Screens The programming screens are divided into separate menus.

3.2.3 Run Screens The run screen appears once a program has been loaded and the

3.2.4 Selecting Operating Parameters

3.3 Main Menus There are four main menu screens. Access Menu 1 by pressing

3.4 Menu One Menu 1 provides programming options for units of measure,

3.4.2 Pump Selection (if multiple pumps)

3.4.3 Refill The refill option allows you to set the refill rate or set the pump

3.4.4 Power Failure [STOP] This feature allows you to set the activity of the pump in the event of a power failure. Press

3.4.6 Display Contrast From the menu, you can adjust the screen brightness for your light conditions and viewing angle.

3.5 MenuTwo Menu 2 provides programming options for serial communication,

3.5.1 Serial Option The serial option menu allows you to set the baud rate and the unit identification number.

3.5.2 Pump Status This option displays the controller software revision, and model of connected pump(s).

3.5.3 External Control The pump pressure or flow rate operation can be controlled externally with an analog voltage. Complete instructions for this feature are provided in Sections 3.12 through 3.12.5.

3.5.4 Multiple Pumps One controller can control up to four pumps at once, either together or independent of each other.

3.5.5 Total Volume Reset This option resets the total volume display of multi-pump pair AB or multi-pump pair CD to zero when operating in continuous flow or modifier mode.

3.6 MenuThree Menu 3 provides programming options for poor fills, diagnostics,

3.6.1 Poor Fill Alarm In constant pressure mode, this feature allows you to set a fill point as a percentage of pump volume. If this volume percentage is not reached after a refill and re-pressurization, the system issues an alarm and stops the pump.

3.6.2 Diagnostic Menu This selection displays an additional menu containing testing options for the system. Refer to Section 9.5 for a complete expla­nation of how to use the diagnostic menu.

3.6.3 Pressure Calibration This is a stored psi value to ensure optimal operation of the pressure transducer. On most pumps, this value, known as the “midpoint adjustment,” can be found on a tag on the transducer cable (shown below).

3.6.4 Diff. Modes This feature allows the use of other analog inputs for pressure input to the pump. The standard pressure transducer MUST be connected to pump in order for this feature to work.

3.7 Menu Four

3.7.1 Modbus Options Beginning with Rev. 1.05 firmware, the Modbus RTU communi-

3.8 Front Panel Keys In addition to the menu options, certain functions and modes are

3.8.2 PRGM GRAD This key is used to place the pump system in gradient mode. For

3.8.3 HOLD The HOLD key is used while a gradient is running. When HOLD

3.8.4 RECALL The RECALL key can only be used when the pump(s) are stopped

3.8.5 STORE The STORE key is operational in the programmed gradient mode. It is used to save the program gradient parameters and exit the programming (parameter entry) mode.

3.8.6 REFILL The REFILL key manually places the pump in refill mode.

3.8.7 LIMITS The controller allows the user to set the minimum and maximum

3.8.9 DISP The DISP key activates Dispense Mode, for applications such as reactant feed and batch delivery, where a specified volume is pumped. Refer to Section 3.10.3 for programming steps.

3.8.10 ACC CTRL The ACC CONTROL key will allow you to manually operate accessories, such as valves, via the Digital Output terminals on the back of the controller.

3.8.11 ZERO PRESS The ZERO PRESSURE key will correct pressure sensor drift. Before pressing ZERO PRESS, the pump should have port fit­tings installed, and be depressurized.

3.9 Control of Multiple Pumps

3.9.1 Multi-Pump Operation A D Series continuous flow pumping system in constant flow mode will consist of two syringe pumps and a valve package, all regulated by one controller. Installation and operating instruc­tions for this system are provided in Section 5.

3.10 Operating Modes The pump has three delivery modes and one refill mode.

3.10.1 Constant Flow To set constant flow operation, use the following procedure:

3.10.2 Constant Pressure Programming a constant pressure operation only requires a few keystrokes. Use the following procedure:

3.10.3 Dispense Mode For applications such as reactant feed and batch delivery where a specified volume is pumped, Dispense Mode is available with Teledyne Isco syringe pumps.

3.11 External Control The pump can be externally controlled for pressure or flow rate

3.12 External Control: Analog

3.12.2 Preparation Before programming the controller to accept the analog signal, you must know the maximum flow rate (constant flow) or the maximum pressure (constant pressure).

3.12.4 Calculation Examples Flow Rate – If MAX FLOW is set at 25 ml/min in the LIMITS menu and the desired scale factor is 5.0 volts per 20 ml/min, do the fol­lowing to determine the analog voltage range:

3.12.5 Setup First, select the desired operating mode by pressing CONST PRESS or CONST FLOW.

3.6.2 Diagnostic Menu This selection displays an additional menu containing testing options for the system. Refer to Section 9.5 for a complete explanation of how to use the diagnostic menu.

3.8.11 ZERO PRESS The ZERO PRESSURE key will correct pressure sensor drift. Before pressing ZERO PRESS, the pump should have port fittings installed, and be depressurized.

3.9.1 Multi-Pump Operation A D Series continuous flow pumping system in constant flow mode will consist of two syringe pumps and a valve package, all regulated by one controller. Installation and operating instructions for this system are provided in Section 5.

3.12.4 Calculation Examples Flow Rate – If MAX FLOW is set at 25 ml/min in the LIMITS menu and the desired scale factor is 5.0 volts per 20 ml/min, do the following to determine the analog voltage range: