Tecan Sunrise User manual

4 (1)

TECAN

Instructions For Use for

SUNRISE Absorbance Reader

Document Part No. I 137 311

2003-12

Document Revision No. 1.0

Tecan Affiliates and Service Centers

Austria

 

Austria

 

Asia

Tecan Austria GmbH

Tecan Sales International GmbH

Tecan Asia (Pte) Ltd.

Untersbergstrasse 1a

Untersbergstrasse 1a

80, Marine Parade #13-04

A-5082 Grödig / Salzburg

A-5082 Grödig / Salzburg

Singapore 449269

Austria

 

Austria

 

Singapore

Tel.: +43

62 46 89 33

Tel.: +43

62 46 89 33

Tel.: +65 44 41 886

Fax: +43

62 46 72 770

Fax: +43

62 46 72 770

Fax: +65 44 41 836

Belgium

France

Germany

Tecan Benelux B.V.B.A.

Tecan France S.A.

Tecan Deutschland GmbH

Vaartdijk 55

Parc d'Activités de Pissaloup

Theodor-Strom-Straße 17

B-2800 Mechelen

Batiment Hermes II

D-74564 Crailsheim

Belgium

Rue Edouard Branly

Germany

 

Tel.: +32 15 42 13 19

F-78190 Trappes

Tel.: +49

79 51 94 170

Fax. +32 15 42 16 12

France

Fax: +49

79 51 50 38

 

Tel.: +33 1 30 68 81 50

 

 

 

Fax: +33 1 30 68 98 13

 

 

Italy

 

Japan

Netherlands

Tecan Italia S.r.l.

Tecan Japan Co. Ltd

Tecan Benelux B.V.B.A.

Via F.lli Cervi

Meiji Seimei Fuchu Building 10F

Industrieweg 30,

Palazzo Bernini

1-40 Miyamachi

NL-4283 Giessen,

Centro Direzionale Milano2

Fuchu City, Tokyo

Netherlands

20090 Segrate (Mi)

Japan

Tel.: +31 018 34 48 17 4

Italy

 

Tel.: +81 42 334 88 55

Fax: +31 018 34 48 06 7

Tel.: +39

02 215 21 28

Fax: +81 42 334 04 01

 

Fax: +39

02 215 97 441

 

 

Spain

Sweden

Switzerland

Tecan Spain

Tecan Nordic AB

Tecan Sales Switzerland AG

Sabino de Arana, 32

Box 208, SE-431 23

Seestrasse 103

E-08028 Barcelona

Mölndal

CH-8708 Männedorf

Spain

Sweden

Switzerland

Tel.: +34 93 490 01 74

Tel: +46 31 75 44 000

Tel: +41 922 8 922

Fax: +34 94 411 24 07

Fax: +46 31 75 44 010

Fax: +41 922 8 923

 

 

United Kingdom

USA

Tecan UK

Tecan US

Theale Court

P.O. Box 13953

11-13 High Street

Research Triangle Park,

Theale

NC 27709

UK-Reading RG7 5AH

USA

United Kingdom

Tel.: +1 919 361 5200

Tel.: +44 11 89 300 300

Fax: +1 919 361 5201

Fax: +44 11 89 305 671

 

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Instructions For Use for SUNRISE Absorbance Reader No. I 137 311 Rev No. 1.0

2003-12

WARNING

CAREFULLY READ AND FOLLOW THE INSTRUCTIONS PROVIDED IN

THIS PUBLICATION BEFORE OPERATING THE INSTRUMENT.

Notice

Every effort has been made to avoid errors in text and diagrams; however, Tecan Austria GmbH assumes no responsibility for any errors, which may appear in this publication.

It is the policy of Tecan Austria GmbH to improve products as new techniques and components become available. Tecan Austria GmbH therefore reserves the right to change specifications at any time.

We would appreciate any comments on this publication.

Manufacturer

Tecan Austria GmbH

Untersbergstraße 1A A-5082 Grödig/Salzburg AUSTRIA / EUROPE

Telephone: 0043 (0)6246/8933 FAX: 0043 (0) 6246/72770 E-mail: office.austria@tecan.com

Copyright Information

The contents of this publication are the property of Tecan Austria GmbH and are not to be copied, reproduced or transferred to another person or persons without prior written permission.

Copyright Tecan Austria GmbH All rights reserved.

Printed in Austria.

Declaration for EU Certificate

See the back of this publication.

SUNRISE Absorbance Reader Intended Use

See chapter 2.2.1 Sunrise Intended Use.

About the Instructions For Use

This publication is intended as Instructions For Use for the SUNRISE Absorbance Reader, which is designed to measure the light absorbance (optical density) of samples in 96 well microplates. It is intended as reference and instruction for the user.

This document instructs how to:

Install the instrument

Clean and maintain the instrument

Warranty

3 Year Warranty

As an expert for microplate instrumentation Tecan proves its commitment to quality and offers a unique 3 year warranty for your SUNRISE Absorbance Reader as a standard. Warranty invalid if instrument is opened or modified.

2003-12

Instructions For Use for SUNRISE Absorbance Reader No. I 137 311 Rev No. 1.0

3

Warnings, Cautions and Notes

There are various types of notices used in this publication. These notices highlight important information or warn the user of a potentially dangerous situation. The notices used in this publication appear below:

Note:

Gives helpful information.

Caution

Indicates a possibility of instrument damage or data loss if instructions are not followed.

WARNING

INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY, LOSS

OF LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS ARE NOT

FOLLOWED.

WARNING

INDICATES THE POSSIBLE PRESENCE OF BIOLOGICALLY

HAZARDOUS MATERIAL. PROPER LABORATORY SAFETY

PRECAUTIONS MUST BE OBSERVED.

WARNING

INDICATES LASER. DO NOT STARE INTO THE BEAM!

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Instructions For Use for SUNRISE Absorbance Reader No. I 137 311 Rev No. 1.0

2003-12

Symbols

 

 

Manufactured by

 

 

 

 

 

Date of manufacture

 

 

 

 

 

Communauté Européenne

 

 

 

 

 

 

 

I V D

In vitro diagnostic medical device

 

 

 

 

 

Laser

Biohazardous

2003-12

Instructions For Use for SUNRISE Absorbance Reader No. I 137 311 Rev No. 1.0

5

Abbreviations

Abbreviation

A

Ampere

Abs

Absorbance

C

Celsius

CE

Communauté Européenne

cm

Centimeter

F

Fahrenheit

Hz

Hertz

IVD

In vitro diagnostics

IVD-D

In vitro diagnostics Directive

kg

Kilogram

l; L

Liter

MB

Megabyte

µL

Microliter

mL

Milliliter

nm

Nanometer

REF

Reference Number/ Order Number

SN

Serial Number

TYPE

Name and type of instrument

V

Volts

VA

Volt ampere

VOLTAGE

Voltage

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Instructions For Use for SUNRISE Absorbance Reader No. I 137 311 Rev No. 1.0

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Table of Contents

 

1.

Safety

 

 

 

 

1.1

Instrument Safety .................................................................

1-1

 

2.

General

 

 

 

2.1

Introduction...........................................................................

2-1

 

 

2.2

Area of Application...............................................................

2-3

 

 

2.2.1

Sunrise Intended Use .............................................................

2-3

 

 

2.2.2

Sunrise Variations ..................................................................

2-4

 

 

2.2.3

Available Options for SUNRISE..............................................

2-5

 

 

2.3

Specifications .......................................................................

2-6

 

 

2.3.1

General...................................................................................

2-6

 

 

2.3.2

SUNRISE Standard Instrument (Standard Filter) ...................

2-7

 

 

2.3.3

SUNRISE Touchscreen Instrument ........................................

2-8

 

 

2.3.4

SUNRISE Tuneable Wavelength (Gradient Filter)..................

2-9

 

 

2.3.5

SUNRISE 6 Filter..................................................................

2-10

 

 

2.3.6

Temperature Control ............................................................

2-10

 

 

2.3.7

Barcode Laser Scanner........................................................

2-10

 

 

2.3.8

Microplates ...........................................................................

2-11

 

 

2.4

Instrument Description ......................................................

2-12

 

 

2.4.1

Back Panel Connections.......................................................

2-13

 

 

2.4.2

Handling the Microplate........................................................

2-14

 

 

2.5

Filter Carriage Description.................................................

2-15

 

 

2.5.1

SUNRISE Standard Filter Carriage ......................................

2-15

 

 

2.5.2

SUNRISE Gradient Filter Carriage .......................................

2-15

 

 

2.5.3

SUNRISE 6 Filter Carriage...................................................

2-16

 

 

2.6

Instrument Features ...........................................................

2-16

 

 

2.6.1

Measurement Modes............................................................

2-16

 

 

2.6.2

Microplate Shaking ...............................................................

2-17

 

 

2.7

Instrument Accessories.....................................................

2-18

 

 

2.8

Software for the SUNRISE Absorbance Reader...............

2-18

 

3.

Installation Procedure

 

 

 

3.1

Introduction...........................................................................

3-1

 

 

3.2

Unpacking and Inspection ...................................................

3-1

 

 

3.3

Unpacking Procedure...........................................................

3-2

 

 

3.4

Power Requirements............................................................

3-2

 

 

3.5

Environmental Requirements..............................................

3-3

 

 

3.6

Instrument Installation Procedure ......................................

3-3

 

 

3.7

Installation of Instrument Control Software .......................

3-3

 

 

3.8

Defining the SUNRISE Instrument Settings .......................

3-4

 

 

3.8.1

Installation of SUNRISE Instrument Settings Software...........

3-4

 

 

 

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Instructions For Use for SUNRISE Absorbance Reader No. I 137 311 Rev No. 1.0

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3.8.2

Starting the SUNRISE Instrument Settings .............................

3-4

 

 

3.8.3

Define Instrument Mode .........................................................

3-5

 

 

3.8.4

Define Filter ............................................................................

3-5

 

 

3.8.5

Define Measurement Mode ....................................................

3-6

 

4.

SUNRISE Touchscreen

 

 

 

4.1

Introduction ...........................................................................

4-1

 

 

4.2

Area of Application for the Touchscreen Option ...............

4-2

 

 

4.3

Specifications .......................................................................

4-2

 

 

4.4

Instrument Description ........................................................

4-2

 

 

4.4.1

Data Handling .........................................................................

4-3

 

 

4.4.2

Printer Compatibility ...............................................................

4-4

 

 

4.5

Operating the Touchscreen .................................................

4-5

 

 

4.5.1

Introduction .............................................................................

4-5

 

 

4.5.2

Starting the Software ..............................................................

4-5

 

 

4.5.3

Special Features of MagellanCE ............................................

4-6

 

5. Error Messages and Trouble Shooting

 

 

 

5.1

Introduction ...........................................................................

5-1

 

 

5.1.1 Table of Error Messages and Trouble Shooting for SUNRISE

 

 

Mode

...................................................................................................

5-1

 

 

5.1.2 Table of Error Messages and Trouble Shooting for SPECTRA

 

 

Mode ...................................................................................................

 

5-4

 

6.

Maintenance & Cleaning

 

 

 

6.1 ...........................................................................

Introduction

6-1

 

 

6.2 ...............................................................

Lamp Replacement

6-1

 

 

6.3 ...............................................................

Filter Replacement

6-3

 

 

6.3.1 ........................................................

Standard Filter Carriages

6-3

 

 

6.3.2 ........................................................

Gradient Filter Carriages

6-4

 

 

6.3.3 ....................................................................

6 Filter Carriages

6-4

 

 

6.4 ................................................................

Fuse Replacement

6-5

 

 

6.5 .......................................................

Cleaning the Instrument

6-6

 

 

6.6 ........................

Preventive Maintenance Plan for SUNRISE

6-6

 

 

6.6.1 .......................................................................................

Daily

6-6

 

 

6.6.2 ....................................................................................

Weekly

6-6

 

 

6.6.3 ....................................................................

Every Six Months

6-6

 

 

6.6.4 .......................................

Yearly (Service Engineer Required)

6-6

 

 

6.6.5 ....................................................................

Every Four Years

6-6

 

 

6.7 ........................................................

Instrument Disinfection

6-7

 

 

6.7.1 ...........................................................

Disinfection Procedure

6-7

 

 

6.8 .......................................................

Disinfection Declaration

6-8

 

 

6.9 .........................................................

Disposal of Instrument

6-9

 

 

6.9.1 .............................................................................

Introduction

6-9

 

 

 

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Instructions For Use for SUNRISE Absorbance Reader No. I 137 311 Rev No. 1.0

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6.9.2 Disposal of Packing Material ..................................................

6-9

6.9.3

Disposal of Operating Material ...............................................

6-9

6.9.4

Disposal of the Sunrise Instrument.......................................

6-10

7. Performance Testing / Quality Control

7.1

Introduction...........................................................................

7-1

7.2

Optimizing for Maximum Performance...............................

7-1

7.2.1

Instrument Location ................................................................

7-1

7.2.2

Operating Procedure ..............................................................

7-2

7.2.3

Self Check Procedure.............................................................

7-2

7.3

Performance Tests ...............................................................

7-3

7.3.1

QC PAC 2...............................................................................

7-3

7.3.2

Microplate Test .......................................................................

7-3

7.3.3

High Meniscus Liquids............................................................

7-5

7.4

Quality Control Testing........................................................

7-8

7.4.1

Precision Testing ....................................................................

7-8

7.4.2

Instrument Accuracy...............................................................

7-9

7.4.3

Instrument Linearity ..............................................................

7-10

Index

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Instructions For Use for SUNRISE Absorbance Reader No. I 137 311 Rev No. 1.0

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1. Safety

1. Safety

1.1Instrument Safety

1.Always follow basic safety precautions when using this product to reduce the risk of injury, fire or electrical shock.

2.Read and understand all information in this document. Failure to read, understand and follow the instructions in this publication may result in damage to the product, injury to operating personnel or poor instrument performance.

3.Observe all WARNINGS and CAUTION statements in this publication.

4.Never open SUNRISE while the instrument is plugged into a power source.

5.Observe proper laboratory safety precautions, such as wearing protective clothing and using approved laboratory safety procedures.

WARNING

IF THE SUNRISE ABSORBANCE READER OR THE MAGELLAN

SOFTWARE ARE MODIFIED IN ANY WAY, THE PERFORMANCE OF THE

READER MAY BE NEGATIVELY AFFECTED,

THE WARRANTY WILL NO LONGER BE VALID AND THE INSTRUMENT

WILL NOT BE CE CONFORM.

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1-1

2. General

2. General

2.1 Introduction

Note:

SUNRISE Absorbance Reader is intended for use with only external software.

Caution

Before starting measurements, make sure that the microplate position A1 is inserted correctly.

The SUNRISE instruments are fully automatic, microprocessor controlled readers designed for professional use, enabling the user to measure the light absorbance (optical density) of samples in 96 well microplates according to the specifications described in this publication for in vitro diagnostics.

Note:

Results obtained using the SUNRISE are influenced by the proper use of the instrument and microplates, according to the instructions given in this document, as well as the liquid compounds used (reagents, chemistry). The instructions for use, storage and other manipulations in connection with samples or reagents must be strictly followed. Taking this fact into consideration, results must be interpreted carefully.

By reading twelve wells simultaneously, the instrument is able to measure a microplate in approximately six seconds.

The obtained transmission values are converted into OD values according to the following formula:

Transmission T = I Io

OD =Log T1

Io = emitted light I = detected light

With an innovative range of options, this versatile microplate reader gives diagnostic and research laboratories all the features for numerous purposes.

Based on a new design concept EPAC, the excellent optical performance and high quality of the SUNRISE will guarantee fast, reproducible and accurate measurements.

The SUNRISE is designed to be fitted into Tecan robotic systems.

The SUNRISE Absorbance Reader is an optical 96-well microplate reader for the measurement of absorbance and turbidimetry of samples from the biological or non-biological origin according to the specifications described in this document, intended for professional use only.

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2. General

The SUNRISE Absorbance Reader is available with options Touchscreen, tuneable wavelength, temperature control and barcode scanner.

The Tecan Sunrise Absorbance Reader when used with the appropriate Magellan software is declared suitable for in-vitro diagnosis (IVD) purposes (Directive 98/79/EC of the European Community).

Magellan is a reader control and data reduction software package, also used for concentration and titer determination.

Note:

It is important to note that the proper installation of the instrument and Magellan software alone will not ensure compliance with the IVD Directive. A range of policies and standard operating procedures according to local regulations must also be established.

Make sure that the instrument and the software are suitable for use with the reagents, chemicals and microplates used (see 7.2 Optimizing for Maximum Performance and 7.3 Performance Tests).

Caution

If the instructions given in this publication are not correctly followed, the instrument may become damaged or procedures may not be correctly performed and the safety of the instrument cannot be guaranteed.

Note:

For more information about the operating instructions, see Magellan or XFluor4 Instructions for Use.

WARNING

CAREFULLY READ AND UNDERSTAND ALL INFORMATION IN THIS DOCUMENT. FAILURE TO READ, UNDERSTAND AND FOLLOW THE INSTRUCTIONS IN THIS PUBLICATION MAY RESULT IN DAMAGE TO THE PRODUCT, INJURY TO OPERATING PERSONNEL OR POOR INSTRUMENT PERFORMANCE.

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2. General

2.2 Area of Application

2.2.1 Sunrise Intended Use

The Sunrise Absorbance Reader is an optical 96-well microplate reader for the measurement of absorbance including turbidimetry (optical density) of samples from biological or non-biological origin according to the specifications described in this manual intended for professional use in:

in-vitro diagnostic environments with tests like:

Enzyme Immuno Assays (EIA), e.g. for diagnostic of infectious diseases, autoimmune diagnostic, allergy screening,

Agglutination Assays, e.g. for blood typing assays,

Enzyme Assays, e.g. haemostaseology assays and

Enzyme Linked Probe Hybridization Assays (ELPHA), e.g. HLAtyping assays,

which have been validated according to the IVD directive or other relevant regulations or

pharmaceutical, biotechnological and life science industry, for research use, for food analysis and veterinary applications.

The Sunrise Absorbance Reader has been validated for use with representative applications from the above list. The validation shows the general ability of the Sunrise Absorbance Reader to use the above mentioned methods.

Note

If the Sunrise Absorbance Reader or Magellan software are modified in any way the instrument loses its guarantee and will no longer be IVD compliant.

Note:

The operating authority must use only CE-labeled test kits for clinical diagnostic applications. The operating authority must assure that the combination of a particular CE-labeled test kit used with the CE-labeled Sunrise Absorbance Reader as been validated according to the IVD directive or to other relevant national or local regulations.

If the Sunrise Absorbance Reader is used with any other software other than Magellan, additional validation must be carried out by the operating authority to ensure the safety of the results. The operating authority is fully responsible for any results obtained using software other than Magellan with the Sunrise Absorbance Reader.

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2-3

2. General

2.2.2 Sunrise Variations

All variations of the Sunrise Absorbance Reader and its Options are compliant with the IVD directive and part of the CE declaration of conformity.

Sunrise Hardware Variations

The following table shows the compatibility of the filter slides with each

Sunrise hardware variation:

Sunrise

4 Filter Slide

6 Filter Slide

Gradient Filter

Variations

Slide

 

 

Standard

X

 

 

 

 

 

 

6 Filter

X

X

 

 

 

 

 

Tuneable

X

 

X

Wavelength

 

 

 

 

Sunrise Options

The Sunrise options work with all hardware variations and accessories and can be used in any combination:

Sunrise Options

Temperature Control

Touch screen

Barcode

Sunrise Accessories

The Sunrise accessories work with all hardware variations and options and can be used in any combination:

Sunrise

IVD Compliant

Research Use

None/ Unrelated3

Accessories

Included in CE1

Separate CE2

Only

4 Filter Slide

X

 

 

 

6 Filter Slide

X

 

 

 

Gradient Filter

X

 

 

 

Sunrise Instrument

 

 

 

X

Settings

 

 

 

 

 

 

 

XFluor 4

 

 

X

 

Magellan

 

X

 

 

Magellan TS

 

X

 

 

QC Pac 1

 

 

 

X

QC Pac 2

 

 

 

X

1IVD compliant. Included in the CE declaration for the instrument.

2IVD compliant. CE declaration is separate from that of the instrument.

3Can be used in an IVD environment. CE declaration does not exist or it is unrelated to IVD compliance.

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Tecan Sunrise User manual

2. General

2.2.3 Available Options for SUNRISE

The SUNRISE is a modular system, so you can create your own tailor-made instrument that meets exactly your needs. Options such as a Touchscreen combined with a WindowsCE based on-board software, free wavelength selection, temperature control and a bar code scanner can be added onto the basic system which is dedicated to remote controlled computer operation.

Factory Installed Options

Touchscreen

B037302

SUNRISE Absorbance Reader

Tuneable wavelength from 400 – 700 nm

B037306

Temperature Control

B037303

Upgrade in Field Possible

Barcode scanner

B037304

Barcode Upgrade Kit

S 039 386

6 Filter

B03641002

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2-5

2. General

2.3 Specifications

The tables below lists the specifications for the instruments.

2.3.1 General

For all instrument options:

 

 

 

PARAMETERS

CHARACTERISTICS

 

Mains Power Input

100 - 120 & 220 - 240 V ± 10% 50/60 Hz

 

(Auto sensing)

Consumption

Stand by mode: approx. 50 VA

 

Operational mode: max. 110 VA

Fuse Rating

2 x F 2.0 A / 250 V (Fast Blow)

Outside Dimensions

Width:

33,5 cm

 

Depth:

32,0 cm

 

Height:

17,0 cm

Weight

6 kg

 

 

Ambient Temperature:

 

 

 

Operation

15°C to 35°C (59°F to 95°F)

Storage

-40°C to 60°C (-40°F to 140°F)

Relative Humidity:

 

 

 

Operation

20 % to 90 %

Storage

5 % to 95 %

Overvoltage Category

II

 

 

Pollution Degree

2

 

 

Method of disposal

Contaminated waste

Environment

See 3.5 Environmental Requirements for

 

more information.

Stability:

After 15 minutes warm up time

Normal measurements

max.

+/- 0.001 Abs

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2. General

2.3.2 SUNRISE Standard Instrument (Standard Filter)

PARAMETERS

CHARACTERISTICS

Measurement time:

 

 

dual wavelength

8 seconds

single wavelength

6 seconds

Wavelength Range

 

 

Standard

340 - 750 nm

Filter Wavelength Accuracy

Central wavelength +/- 2 nm

Filter Bandwidth

 

 

At 50% transmission:

10 +/- 2nm

Measurement Range:

 

 

340 – 399 nm

0 - 3.000 Abs

400 – 750 nm

0 - 4.000 Abs

Resolution:

0.001 Abs.

Accuracy:

 

 

(492 nm) 0.000 - 2.000 Abs

Better than +/- 1.0 % +/- 0.010 Abs.

(492 nm) 2.000 - 3.000 Abs

Better than +/- 1.5 % +/- 0.010 Abs.

Precision:

 

 

(492 nm) 0.000 - 2.000 Abs.

Better than +/- 0.5 % +/- 0.005 Abs

(492 nm) 2.000 - 3.000 Abs.

Better than +/- 1.0 % +/- 0.005 Abs

Linearity:

 

 

(340 - 399 nm) 0.000 - 2.000 Abs.

Better than +/- 2 %

(400 - 750 nm) 0.000 - 2.000 Abs.

Better than +/- 1 %

2.000 - 3.000 Abs.

Better than +/- 1.5 %

Wavelength selection:

 

 

Standard filter

Narrow band interference filters. Up to four

 

filters can be mounted in a filter carriage.

 

The instrument can use up to eight different

 

filter carriages.

Light source:

Halogen lamp 20 W

All connected devices must

 

 

be approved and listed as per

 

 

EN 60950, UL 1950 or

 

 

CSA C22.2 No. 950 for Data

 

 

Processing Devices.

 

 

Computer interface:

 

 

Serial RS 232 C

300 - 38,400 baud

Printer

Only functions with the Touchscreen

 

option.

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2. General

2.3.3 SUNRISE Touchscreen Instrument

PARAMETERS

CHARACTERISTICS

 

Screen Size

240 x 320 pixel

Operating System

WindowsCE

Operating Software:

MagellanCE

Storage

8 / 32 MB (SUNRISE Memo Card)

Computer interface:

 

 

Serial RS 232 C

300 - 38,400 baud

Printer

Only functions with the Touchscreen option.

Serial RS 232 C to the Touchscreen

Only for the XChange program.

MagellanCE software

See Magellan Manual

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2. General

2.3.4 SUNRISE Tuneable Wavelength (Gradient Filter)

PARAMETERS

CHARACTERISTICS

Measurement time:

 

 

dual wavelength

8 seconds

single wavelength

6 seconds

Wavelength Range

 

 

Gradient filter

400 - 700 nm

Standard filter

340 - 399 nm & 700 - 750 nm

Measurement Range:

 

 

400 – 750 nm

0 - 4.000 Abs.

340 – 399 nm

0 - 3.000 Abs

Resolution:

0.001 Abs.

Accuracy:

 

 

(492 nm) 0.000 - 2.000 Abs

Better than +/- 1.5 % +/- 0.010 Abs.

Precision:

 

 

(492 nm) 0.000 - 2.500 Abs

Better than +/- 1.0 % +/- 0.005 Abs

Linearity:

 

 

(492 nm) 0.000 - 2.500 Abs.

Better than +/- 2 %

Wavelength selection:

 

 

Gradient filter

Special gradient filter any wavelength

 

between 400 and 700 nm in 1 nm steps.

 

The instrument can use up to eight different

 

filter carriages

Filter Wavelength Accuracy

Central wavelength +/- 2 nm

Filter Bandwidth

 

 

At 50% transmission:

 

 

450 nm

10 – 16 nm

550 nm

10 – 14 nm

650 nm

10 – 18 nm

2003-12

Instructions For Use for SUNRISE Absorbance Reader No. I 137 311 Rev No. 1.0

2-9

2. General

2.3.5 SUNRISE 6 Filter

See 2.3.2 SUNRISE Standard Instrument (Standard Filter)

2.3.6

Temperature Control

 

 

 

 

Peltier-based temperature control.

 

 

 

 

PARAMETERS

CHARACTERISTICS

 

 

 

Temperature Range:

Room temperature up to 42°C increments

 

 

 

of 0.1°C

 

 

Accuracy

+/- 0.2°C

 

 

Preheating Time

20 min.

2.3.7

Barcode Laser Scanner

 

 

WARNING

LASER RADIATION – DO NOT STARE INTO THE BEAM!

CLASS II / 2 LASER PRODUCT.

The Class II / 2 Laser Scanner corresponds to the following norms:

DIN EN 60825-1 : 2001

CDRH 21 CFR 1040.10

PARAMETERS

CHARACTERISTICS

Classification

Class II / 2 Laser Product

Input Power

5V DC +/- 10%

Emission Duration

> 0.25s

Scan Rate

42 +/- 3 Scans / second (bi-directional)

Laser Power

< 1mW

Definition of depth

Max. 40cm

Resolution

0.15cm

Min. Print Contrast

25% at 675nm

Ambient Light

Sun Light: 40000 Lux

 

Halogen Light: 1500 Lux

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Instructions For Use for SUNRISE Absorbance Reader No. I 137 311 Rev No. 1.0

2003-12

2. General

2.3.8 Microplates

Only 96-well format microplates (v-shaped, flat and round including strip-wells) can be used with the Sunrise Absorbance Reader.

PARAMETERS

CHARACTERISTICS

Max. overall plate height

14.35 mm ± 0.76 mm

 

(0.5650 inches ± 0.0299 inches)

Diameter of wells

9.0 mm (0.3543 inches)

Pitch size (center to center)

9.0 mm (0.3543 inches)

Bottom shape

V-shaped, Flat and Round bottom

The barcode sticker has to be positioned on the right hand side of the plate, 7 mm from the front-right edge. The barcode may be up to 48 mm long.

6

A1

55

7

The SUNRISE barcode reader supports the following barcode types:

CODE 39

Interleaved 2 of 5

UPC A, UPC E0, UPC E1

EAN8, EAN 13

Codabar

CODE 128

2003-12

Instructions For Use for SUNRISE Absorbance Reader No. I 137 311 Rev No. 1.0

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